Rh5BG295100

NAD(P)H-quinone oxidoreductase chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0020222
Physical Location & Seq
Forward (+)
37619968 .. 37621930
1963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG295100.1

Sequence Viewer

Length: 765 bp
ATGCTTTTAGCCGGAATCTTATTAAAAATGGGAGCGTATGGGTTGGTTCGGATCAATATGGAATTATTACCCCATGCCCATTCTATATTTTCTCCCTGGTTGATGATAGTGGGCACAATTCAAATTATTTATGCAGGTTTAACATCTCCTGGTCAGCGTAATTTAAAAAAAAGAATAGCCTATTCCTCTGTATCTCATATGGGTTTCATAATTATAGGAATTGGTTCTATAAGCGATACAGGGCTCAATGGGGCCATTTTACAAATAATTTCTCACGGATTTATTGGCGCTGCGCTTTTTTTCTTGGCCGGAACGAGTTATGATAGAATACGACTTATTTATCTCGACGAAATGGGCGGAATGGCTATCGCAATTCCAAAAATATTCACGACTTTCAGTATCTTATCAATGGCTTCGCTTGCATTACCGGGCATGAGCGGTTTTGTTGCCGAATTGATAATTTTTTTTGGAATACTTACTAGCCAAAAATATCTTTTAATGACAAAAGTATTAATTACTTTTGTAATGGCAATTGGAATGATATTAACTCCTATTTATTCATTATCTATGTTACGCCAGATGTTCTATGGATACAAGCTTTTTAATGCCCCAAACTCTTATTTTTTTGATTCTGGACCGCGAGAGTTAGTTATTTCTATTTCTATCCTTTTACCTGTAATAGGTATTGGTATTTATCCCGATTTCGTTTTCTCATTATCAGTTGACAAGGTCGAAGCTATTATATCAAATTTTTTTTATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

254

Amino Acids

27.94

Weight (kDa)

9.4

Isoelectric Point (pI)

27.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 1 - 175 9.5e-34 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020222)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01050
malus_domestica MD04G1189100.v1.1
rosa_chinensis RchiOBHm_Chr5g0017231 RchiOBHm_Chr5g0043051 RchiOBHm_CPg0502791
rosa_samantha Rh5BG295100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 125
AccBSI CCGCTC 1 cut(s) 438
AccII CGCG 1 cut(s) 640
AciI CCGC 3 cut(s) 357, 438, 638
AclWI GGATC 1 cut(s) 59
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 748
AfiI CCNNNNNNNGG 1 cut(s) 680
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 2 cut(s) 95, 148
AluBI AGCT 2 cut(s) 598, 737
AluI AGCT 2 cut(s) 598, 737
AlwI GGATC 1 cut(s) 59
AoxI GGCC 2 cut(s) 252, 306
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 1 cut(s) 748
AseI ATTAAT 1 cut(s) 512
Asp700I GAANNNNTTC 1 cut(s) 223
AspLEI GCGC 2 cut(s) 290, 295
AspS9I GGNCC 2 cut(s) 252, 635
AsuC2I CCSGG 1 cut(s) 429
AvaII GGWCC 1 cut(s) 635
BaeGI GKGCMC 1 cut(s) 116
BanII GRGCYC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 277
BciT130I CCWGG 2 cut(s) 97, 150
BciVI GTATCC 1 cut(s) 584
BcnI CCSGG 1 cut(s) 429
BfaI CTAG 1 cut(s) 480
BfoI RGCGCY 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 125
BfuI GTATCC 1 cut(s) 584
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 3 cut(s) 97, 150, 429
Bme18I GGWCC 1 cut(s) 635
BmgT120I GGNCC 2 cut(s) 252, 635
BmiI GGNNCC 1 cut(s) 253
BmrFI CCNGG 3 cut(s) 97, 150, 429
BpuMI CCSGG 1 cut(s) 429
BsaJI CCNNGG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 680
BseBI CCWGG 2 cut(s) 97, 150
BseDI CCNNGG 1 cut(s) 95
BseLI CCNNNNNNNGG 1 cut(s) 680
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 1 cut(s) 277
Bsh1236I CGCG 1 cut(s) 640
BshFI GGCC 2 cut(s) 254, 308
BsiSI CCGG 3 cut(s) 12, 309, 428
BslI CCNNNNNNNGG 1 cut(s) 680
BsnI GGCC 2 cut(s) 254, 308
Bsp1286I GDGCHC 2 cut(s) 116, 246
Bsp143I GATC 1 cut(s) 51
BspACI CCGC 3 cut(s) 357, 438, 638
BspANI GGCC 2 cut(s) 254, 308
BspFNI CGCG 1 cut(s) 640
BspLI GGNNCC 1 cut(s) 253
BspMI ACCTGC 1 cut(s) 125
BspPI GGATC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 97, 150
BstC8I GCNNGC 1 cut(s) 420
BstENI CCTNNNNNAGG 1 cut(s) 678
BstFNI CGCG 1 cut(s) 640
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 290, 295
BstKTI GATC 1 cut(s) 54
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 419
BstNI CCWGG 2 cut(s) 97, 150
BstSCI CCNGG 3 cut(s) 95, 148, 427
BstSLI GKGCMC 1 cut(s) 116
BstUI CGCG 1 cut(s) 640
BstV1I GCAGC 1 cut(s) 277
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 2 cut(s) 254, 308
BveI ACCTGC 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 2 cut(s) 290, 295
Cfr13I GGNCC 2 cut(s) 252, 635
CviAII CATG 2 cut(s) 74, 433
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
DraI TTTAAA 1 cut(s) 165
EaeI YGGCCR 1 cut(s) 306
EciI GGCGGA 1 cut(s) 372
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 635
EcoNI CCTNNNNNAGG 1 cut(s) 678
EcoRII CCWGG 2 cut(s) 95, 148
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 2 cut(s) 77, 436
FatI CATG 2 cut(s) 73, 432
FauNDI CATATG 1 cut(s) 198
Fnu4HI GCNGC 1 cut(s) 291
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 291
FspBI CTAG 1 cut(s) 480
GlaI GCGC 2 cut(s) 289, 294
GluI GCNGC 1 cut(s) 291
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 2 cut(s) 254, 308
HapII CCGG 3 cut(s) 12, 309, 428
HhaI GCGC 2 cut(s) 290, 295
Hin1II CATG 2 cut(s) 77, 436
Hin6I GCGC 2 cut(s) 288, 293
HinP1I GCGC 2 cut(s) 288, 293
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 2 cut(s) 15, 629
HpaII CCGG 3 cut(s) 12, 309, 428
Hpy166II GTNNAC 1 cut(s) 724
Hpy188I TCNGA 1 cut(s) 51
Hpy188III TCNNGA 4 cut(s) 344, 388, 633, 698
Hpy8I GTNNAC 1 cut(s) 724
Hpy99I CGWCG 1 cut(s) 350
HpyCH4V TGCA 2 cut(s) 134, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 419
Hsp92II CATG 2 cut(s) 77, 436
HspAI GCGC 2 cut(s) 288, 293
Kzo9I GATC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 32
Lsp1109I GCAGC 1 cut(s) 277
MaeI CTAG 1 cut(s) 480
MaeIII GTNAC 1 cut(s) 570
MalI GATC 1 cut(s) 53
MbiI CCGCTC 1 cut(s) 438
MboI GATC 1 cut(s) 51
MfeI CAATTG 1 cut(s) 531
MhlI GDGCHC 2 cut(s) 116, 246
MnlI CCTC 1 cut(s) 196
MroXI GAANNNNTTC 1 cut(s) 223
MseI TTAA 7 cut(s) 23, 140, 164, 497, 512, 545, 603
MspI CCGG 3 cut(s) 12, 309, 428
MspR9I CCNGG 3 cut(s) 97, 150, 429
MteI GCGCNGCGC 1 cut(s) 291
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 2 cut(s) 97, 150
MvnI CGCG 1 cut(s) 640
MwoI GCNNNNNNNGC 1 cut(s) 419
NciI CCSGG 1 cut(s) 429
NdeI CATATG 1 cut(s) 198
NdeII GATC 1 cut(s) 51
NlaIII CATG 2 cut(s) 77, 436
NlaIV GGNNCC 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 223
PfeI GAWTC 2 cut(s) 15, 629
PflFI GACNNNGTC 1 cut(s) 728
PkrI GCNGC 1 cut(s) 292
PshBI ATTAAT 1 cut(s) 512
Psp6I CCWGG 2 cut(s) 95, 148
PspGI CCWGG 2 cut(s) 95, 148
PspN4I GGNNCC 1 cut(s) 253
PspPI GGNCC 2 cut(s) 252, 635
PsyI GACNNNGTC 1 cut(s) 728
SaqAI TTAA 7 cut(s) 23, 140, 164, 497, 512, 545, 603
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 1 cut(s) 51
Sau96I GGNCC 2 cut(s) 252, 635
ScrFI CCNGG 3 cut(s) 97, 150, 429
SduI GDGCHC 2 cut(s) 116, 246
SetI ASST 6 cut(s) 139, 600, 676, 685, 732, 739
SinI GGWCC 1 cut(s) 635
SsiI CCGC 3 cut(s) 357, 438, 638
SspI AATATT 1 cut(s) 384
SspMI CTAG 1 cut(s) 480
StyD4I CCNGG 3 cut(s) 95, 148, 427
TaqI TCGA 2 cut(s) 345, 732
TfiI GAWTC 2 cut(s) 15, 629
Tru1I TTAA 7 cut(s) 23, 140, 164, 497, 512, 545, 603
Tru9I TTAA 7 cut(s) 23, 140, 164, 497, 512, 545, 603
TseI GCWGC 1 cut(s) 290
TspDTI ATGAA 2 cut(s) 196, 549
TspGWI ACGGA 1 cut(s) 291
Tth111I GACNNNGTC 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 635
VspI ATTAAT 1 cut(s) 512
XagI CCTNNNNNAGG 1 cut(s) 678
XapI RAATTY 1 cut(s) 748
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmnI GAANNNNTTC 1 cut(s) 223
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.