MD04G1194300.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016705
Physical Location & Seq
Reverse (-)
28365322 .. 28369345
4024 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1194300.v1.1.491

Sequence Viewer

Length: 3267 bp
ATGAAACCGCGGCTGATAAAGTCTTGCTCAAGTGTTCTATGGCGGCCCATTTGTTTTCTCTTCGTTCTGGGCCTTTCGTGCTACATGATCTCAATGTTGTTTAAGATGACGAAAAGAATCGAACATCGTATACTGTTCGAATCACATGAAGCACTCAACAGATCCTTTTCGAGCATCGACTCCACCTCAAAGTTGCTGACTCCATTGAATCCATCAGCAGTTAACCTGGCAAGATCTCTAACTTCGTCACTTAATGGAACCGAGCTCAATTTCACTACCATCCAAACAACGGTTGTGCCAACGTTGTTTCTAGCTCTTTCGACGATTCCACATATCTCACAGGTCTCTTATATAGGATTAGATGGCCTTTTATTCTCACTTAACAACTACGCGGATCAAACTTATGCTGTCTTCTCCAATGCTTCATTTTCTTCAAATTGGTACACTAAACCCGTAAATAGAGACACCGGGAAATTATATGGCACCGCAGTTGCTTCAGATTCTAAGGTGATAGTAAATGCAAGTTGGTTCCAAGCCGCCTTAAACAGCACGAGCGGTTATTCTTGGCTTGGAAGGGGATGGGACAAAGCTCAAGGTAGCTTATTTATAAGTACTGTGGTTATGGATGGCAGAGGTGTGATTTCCCTTGGTATCCCAGCAAAAGTTGTAGTTGATCATTTTGCGGCTTTAGACTTCCATGGGGGTTATTTTCACTTGGCTACTGCAGATGGGGACGTGATTGTACAATCAAAACTTCCGAAAACCCAATTTCAGATCAGCAACAACACCGTTTCGGTGCAAACGGTGAAGCCGAATGGTAATGTTACTAGTAATCTTGGTCACTTCCCATGTAATACTCCTGATGATGACGACAAAGACAATGATGGCAGCAACGGTGGCAAATTGGGATCTTCTTACTCATGGAAACTCAAGGGAACCAAGTATAGGGCGTATTGCTCCACCCTCGAGATCATTGGAGTTCGATCGGTATATGTATTGACTTGTCCTGCCAATGGACTTGCAAACGTTGACCATAAATCGGTTGTGTTGCTTGGCATTCTGCTATTTATAATTCTTGTTTCTGTTTGCATATTCATATTCTTAATAATAAGAGCAGCAGGAAGAGAAATGATTTTATGTGCGCGGATCATAAAACAAGAAGGCGTGACACGTCAGGCAGAGCGCAAGAGTATGAATAAAACTAAAGCCTTCAGTAGAGCAAGCCATGACGTGCGTGCTTCGTTAGCAGCCATCACTGGTTTAATAGAGTTGTGTCCTCAAGATGCCAATCCTGACTCCGAATTAGCAGCCAACTTGGCCCAGATGGATACTTGTACAAATGACCTCCTAGGCATATTGAATTCCGTTCTTGATATAAGCAAGATTGAAGCCGGAAAAGTGCAACTTGAAATTGAAGAATTCAATCTGGCTCAGCTTCTTGAGGATGTCGTTGACATGTTCTATACTATTGCTATCAAGAAAGGAGTTGACATCGTGCTCGATCCTTGTGACGATTCAATTGTCAAAACATGTTATGTGAGAGGCGACAGAGGAAAGCTTAAACAGATAATGTGCAACTTACTCAGCAATGCCGTGAAGTTTACATCTGAAGGTCATATCACAGTTCGTGCCATGGTTCCGAAAACTAGTTATGAAAATGAAATCATCGCTTCCAATAATAATCGCGTGCTGAATTGTTTGTCATGGCTCTTTTACAAGAGTACAGAAGATTTCAACGATTTGGATGCTCTTCAGACATCTCAGAAAGATCCAAACAACACAAAGTTTGTCATTGAGGTCGATGATACAGGTGAGGGAATTCCTAAAGACAAGCAAGAATTTGTTTTCGAAAACTTTGTGCAAGTTACAGACCGGGCTACTGGAAAAGAAGGATCTGGTTTGAGACTTGGTATTGTTGAATCTTTGGTGCGTCTAATGGGCGGAGAAATAAGAATTCTTGATAAAGAGAATGGTGAGAGAGGCACTTGTTTCAGCTTTGATGTTTCTCTTCTAACATGTAAACCCAAGTCTGGTGACATTGAAGAGGAATCCCCTATAATGCATAGTGATCGCTCTGCGAATGCATTTCAACCTTTTGGTATACAACTTTTGGTGCCTTCACCAAAACAAGAGGGGTCTCATGTCGTACTCTTCATAGAAGGCGATGAACGAAGAAAGATTTTGGTGAAGTACATCAGCAGGTTGAACATAAAAGTATCATATGTAAAGTATGCGAACAATCTTCTTCCACAACTTGAGAAGATAAAGCGTAAGTTGGATATTTCCTACTTTAATTATTCAGAAAAATCCCAACAAGTTTTTCATGACCAATTAAGCACATCTGCATTCAAAAACTCGGATTCAAGAGCAAGTGATGGATCTCACAGCACCAAGGAAGAAGATGGACATACTTGTAGGAAGATCAACTCTAGAAATTCACCTGCGAGCAACGTACTCGTTGTGATTGACACCAACGGAGGTGCTTTCTCTGAGCTGTATACAACTATGGCTAGCTTCAGGAAAGACATTCATAACTCAAGATGCAAGGTTGTCTGGCTCGATAGTTCAATCACGCGCAACACGCATCCGCTGCAAATGGAAGGATGTAGCCTAGCTCCTCCAAATGATTACATCATATACAAACCATTTCACGGATCACGCTTACATAAAGTATTGGGACTATTACCTGAACTTAAGAGATGCAACTTGCCAGAACTAGAAACGAGAAAACAAGATCAAGAAGCGCAACAAATAGAGCTTGAGCTCGCAACAAATGAATCAGGAATGTCGTCGTCATTGCAACAAATAACCATTCATAAATGTGTAGAGAAAAAAAGTGACCAGGAGCTCTTGCACGGAAAGAAAGTTCTGGTTGTTGACGATGATCAAGTAACAAGAAAGGTAATTGCTAGTTCTCTTCAAGGACTCGGAGCAGTCGTTGAAGTGTTAGAGAACGGGAAAGAGGCCTTTGAACAAGTTTTCAAAGCTCTAAGCGATCATAGCAAGAAGGGTCATACGAATTCCACCCTCTACGACTACATTTTCATGGATTGTCAGATGCTGATAATGGATGGATTCGCAGCAACAAGACTGATACGGATGGAGGAAAAACAGTACGGCGTTCATCTTCGAATTATCGCGTTAACATGTCATGCGGTAACTGAGGAGGTTAGCAAGACTCTTGAAGCAGGGATGGATGTCCATTTAACCAAACCATTCAAGATGGACAAGTTCATGCGGGTCATCAAATCCTTTGAGGACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

1089

Amino Acids

120.97

Weight (kDa)

6.82

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 400 - 465 1.6e-08 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 594 - 669 1e-13 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Response_reg PF00072 956 - 1082 2e-18 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016705)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47430
malus_domestica MD04G1194300.v1.1
prunus_persica Prupe.6G314200_v2.0.a1
pyrus_communis pycom04g17180
rosa_chinensis RchiOBHm_Chr3g0456951
rosa_laevigata RLG00000025260
rosa_multiflora Rmu_sc0016008.1_g000003
rosa_roxburghii Rroxscaffold_6G00422340
rosa_rugosa Rorug03G0017600
rosa_samantha Rh3AG077600 Rh3BG079500 Rh3CG079400 Rh3DG081200
rosa_wichuraiana Rw3G006650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 608, 1070
AarI CACCTGC 1 cut(s) 2449
Acc36I ACCTGC 2 cut(s) 2190, 2449
AccB1I GGYRCC 2 cut(s) 482, 2113
AccBSI CCGCTC 1 cut(s) 555
AccI GTMKAC 3 cut(s) 130, 2101, 2498
AccII CGCG 6 cut(s) 10, 392, 1144, 1686, 2575, 3140
AclI AACGTT 2 cut(s) 302, 1026
AclWI GGATC 9 cut(s) 156, 402, 916, 1154, 1496, 1763, 1900, 2386, 2662
AcsI RAATTY 7 cut(s) 1360, 1418, 1818, 1838, 1953, 2434, 3019
AcuI CTGAAG 5 cut(s) 480, 1195, 1629, 1736, 2500
AfaI GTAC 9 cut(s) 443, 613, 744, 1336, 1723, 2148, 2192, 2454, 3116
AfiI CCNNNNNNNGG 6 cut(s) 289, 945, 1013, 1039, 2030, 2651
AflII CTTAAG 1 cut(s) 2693
AflIII ACRYGT 5 cut(s) 1169, 1455, 1529, 2015, 3146
AhlI ACTAGT 2 cut(s) 827, 1646
AjiI CACGTC 3 cut(s) 736, 1172, 1231
AjnI CCWGG 2 cut(s) 225, 2841
AjuI GAANNNNNNNTTGG 2 cut(s) 2304, 2336
AloI GAACNNNNNNTCC 2 cut(s) 2850, 2882
Alw21I GWGCWC 4 cut(s) 267, 1500, 2766, 2850
Alw26I GTCTC 4 cut(s) 349, 456, 1897, 2142
AlwI GGATC 9 cut(s) 156, 402, 916, 1154, 1496, 1763, 1900, 2386, 2662
AlwNI CAGNNNCTG 1 cut(s) 3061
Ama87I CYCGRG 1 cut(s) 965
AoxI GGCC 5 cut(s) 44, 70, 364, 1317, 2964
ApeKI GCWGC 6 cut(s) 888, 1115, 1247, 1307, 2590, 3080
ApoI RAATTY 7 cut(s) 1360, 1418, 1818, 1838, 1953, 2434, 3019
AspA2I CCTAGG 1 cut(s) 1348
AspLEI GCGC 4 cut(s) 1144, 1185, 2577, 2746
AspS9I GGNCC 3 cut(s) 45, 70, 1318
AsuC2I CCSGG 2 cut(s) 469, 1874
AsuHPI GGTGA 8 cut(s) 520, 817, 1823, 1985, 2045, 2112, 2197, 2430
AsuII TTCGAA 3 cut(s) 138, 1848, 3130
AsuNHI GCTAGC 1 cut(s) 2510
AvaI CYCGRG 1 cut(s) 965
AvrII CCTAGG 1 cut(s) 1348
BanI GGYRCC 2 cut(s) 482, 2113
BanII GRGCYC 3 cut(s) 267, 2766, 2850
BauI CACGAG 1 cut(s) 550
BbsI GAAGAC 1 cut(s) 403
Bbv12I GWGCWC 4 cut(s) 267, 1500, 2766, 2850
BbvI GCAGC 6 cut(s) 900, 1127, 1259, 1319, 2577, 3092
BceAI ACGGC 2 cut(s) 1577, 3133
BcgI CGANNNNNNTGC 6 cut(s) 1727, 1761, 2051, 2085, 2437, 2471
BciT130I CCWGG 2 cut(s) 227, 2843
BciVI GTATCC 2 cut(s) 662, 1321
BclI TGATCA 2 cut(s) 673, 2884
BcnI CCSGG 2 cut(s) 469, 1874
BcoDI GTCTC 4 cut(s) 349, 456, 1897, 2142
BcuI ACTAGT 2 cut(s) 827, 1646
BfaI CTAG 9 cut(s) 311, 828, 1349, 1647, 2430, 2511, 2612, 2717, 2910
BfmI CTRYAG 1 cut(s) 723
BfrI CTTAAG 1 cut(s) 2693
BfuAI ACCTGC 2 cut(s) 2190, 2449
BfuI GTATCC 2 cut(s) 662, 1321
BglI GCCNNNNNGGC 1 cut(s) 1316
BglII AGATCT 1 cut(s) 233
BlnI CCTAGG 1 cut(s) 1348
BlpI GCTNAGC 1 cut(s) 1431
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 4 cut(s) 227, 469, 1874, 2843
BmeT110I CYCGRG 1 cut(s) 965
BmgBI CACGTC 3 cut(s) 736, 1172, 1231
BmgT120I GGNCC 3 cut(s) 45, 70, 1318
BmiI GGNNCC 6 cut(s) 259, 484, 530, 937, 1638, 2115
BmrFI CCNGG 4 cut(s) 227, 469, 1874, 2843
BmsI GCATC 7 cut(s) 183, 1273, 1735, 2531, 2593, 2690, 3048
BmtI GCTAGC 1 cut(s) 2514
BpiI GAAGAC 1 cut(s) 403
Bpu1102I GCTNAGC 1 cut(s) 1431
Bpu14I TTCGAA 3 cut(s) 138, 1848, 3130
BpuEI CTTGAG 8 cut(s) 13, 576, 914, 1263, 1460, 2276, 2521, 2780
BpuMI CCSGG 2 cut(s) 469, 1874
BsaBI GATNNNNATC 1 cut(s) 1287
BsaI GGTCTC 2 cut(s) 349, 2142
BsaJI CCNNGG 6 cut(s) 8, 646, 697, 1348, 1632, 2391
BsaXI ACNNNNNCTCC 2 cut(s) 2922, 2952
Bsc4I CCNNNNNNNGG 6 cut(s) 289, 945, 1013, 1039, 2030, 2651
Bse1I ACTGG 2 cut(s) 1261, 1885
Bse3DI GCAATG 2 cut(s) 1594, 2795
Bse8I GATNNNNATC 1 cut(s) 1287
BseBI CCWGG 2 cut(s) 227, 2843
BseDI CCNNGG 6 cut(s) 8, 646, 697, 1348, 1632, 2391
BseJI GATNNNNATC 1 cut(s) 1287
BseLI CCNNNNNNNGG 6 cut(s) 289, 945, 1013, 1039, 2030, 2651
BseMI GCAATG 2 cut(s) 1594, 2795
BseMII CTCAG 5 cut(s) 1445, 1597, 1775, 2481, 3153
BseNI ACTGG 2 cut(s) 1261, 1885
BseRI GAGGAG 2 cut(s) 2607, 3179
BseXI GCAGC 6 cut(s) 900, 1127, 1259, 1319, 2577, 3092
BseYI CCCAGC 1 cut(s) 655
Bsh1236I CGCG 6 cut(s) 10, 392, 1144, 1686, 2575, 3140
Bsh1285I CGRYCG 1 cut(s) 986
BshFI GGCC 5 cut(s) 46, 72, 366, 1319, 2966
BshNI GGYRCC 2 cut(s) 482, 2113
BsiEI CGRYCG 1 cut(s) 986
BsiHKAI GWGCWC 4 cut(s) 267, 1500, 2766, 2850
BsiHKCI CYCGRG 1 cut(s) 965
BsiSI CCGG 3 cut(s) 468, 1392, 1873
BslFI GGGAC 3 cut(s) 596, 746, 2691
BslI CCNNNNNNNGG 6 cut(s) 289, 945, 1013, 1039, 2030, 2651
BsmAI GTCTC 4 cut(s) 349, 456, 1897, 2142
BsmFI GGGAC 3 cut(s) 596, 746, 2691
BsmI GAATGC 3 cut(s) 1056, 2086, 2345
BsnI GGCC 5 cut(s) 46, 72, 366, 1319, 2966
Bso31I GGTCTC 2 cut(s) 349, 2142
BsoBI CYCGRG 1 cut(s) 965
Bsp119I TTCGAA 3 cut(s) 138, 1848, 3130
Bsp1286I GDGCHC 4 cut(s) 267, 1500, 2766, 2850
Bsp1407I TGTACA 2 cut(s) 742, 1334
Bsp1720I GCTNAGC 1 cut(s) 1431
Bsp19I CCATGG 2 cut(s) 697, 1632
BspANI GGCC 5 cut(s) 46, 72, 366, 1319, 2966
BspCNI CTCAG 5 cut(s) 1444, 1596, 1774, 2482, 3154
BspFNI CGCG 6 cut(s) 10, 392, 1144, 1686, 2575, 3140
BspHI TCATGA 1 cut(s) 2323
BspLI GGNNCC 6 cut(s) 259, 484, 530, 937, 1638, 2115
BspMAI CTGCAG 1 cut(s) 727
BspMI ACCTGC 2 cut(s) 2190, 2449
BspOI GCTAGC 1 cut(s) 2514
BspPI GGATC 9 cut(s) 156, 402, 916, 1154, 1496, 1763, 1900, 2386, 2662
BspQI GCTCTTC 1 cut(s) 1755
BspT104I TTCGAA 3 cut(s) 138, 1848, 3130
BspT107I GGYRCC 2 cut(s) 482, 2113
BspTI CTTAAG 1 cut(s) 2693
BspTNI GGTCTC 2 cut(s) 349, 2142
BsrBI CCGCTC 1 cut(s) 555
BsrDI GCAATG 2 cut(s) 1594, 2795
BsrGI TGTACA 2 cut(s) 742, 1334
BsrI ACTGG 2 cut(s) 1261, 1885
BssECI CCNNGG 6 cut(s) 8, 646, 697, 1348, 1632, 2391
BssNAI GTATAC 3 cut(s) 131, 2102, 2499
BssSI CACGAG 1 cut(s) 550
BssT1I CCWWGG 5 cut(s) 646, 697, 1348, 1632, 2391
Bst1107I GTATAC 3 cut(s) 131, 2102, 2499
Bst2BI CACGAG 1 cut(s) 550
Bst2UI CCWGG 2 cut(s) 227, 2843
Bst4CI ACNGT 8 cut(s) 135, 292, 616, 790, 805, 896, 1624, 3114
Bst6I CTCTTC 7 cut(s) 65, 1117, 1755, 2013, 2037, 2156, 2922
BstAFI CTTAAG 1 cut(s) 2693
BstAPI GCANNNNNTGC 1 cut(s) 2590
BstAUI TGTACA 2 cut(s) 742, 1334
BstBI TTCGAA 3 cut(s) 138, 1848, 3130
BstC8I GCNNGC 6 cut(s) 1222, 1236, 1688, 2446, 2512, 2766
BstDEI CTNAG 7 cut(s) 504, 1431, 1583, 1761, 2490, 2990, 3162
BstDSI CCRYGG 3 cut(s) 8, 697, 1632
BstFNI CGCG 6 cut(s) 10, 392, 1144, 1686, 2575, 3140
BstHHI GCGC 4 cut(s) 1144, 1185, 2577, 2746
BstMAI GTCTC 4 cut(s) 349, 456, 1897, 2142
BstMCI CGRYCG 1 cut(s) 986
BstMWI GCNNNNNNNGC 7 cut(s) 78, 897, 1244, 1316, 2581, 2590, 3000
BstNI CCWGG 2 cut(s) 227, 2843
BstNSI RCATGY 4 cut(s) 1459, 1533, 2019, 3150
BstSCI CCNGG 4 cut(s) 225, 467, 1872, 2841
BstSFI CTRYAG 1 cut(s) 723
BstUI CGCG 6 cut(s) 10, 392, 1144, 1686, 2575, 3140
BstV1I GCAGC 6 cut(s) 900, 1127, 1259, 1319, 2577, 3092
BstV2I GAAGAC 1 cut(s) 403
BstX2I RGATCY 6 cut(s) 161, 233, 908, 1768, 1892, 2378
BstYI RGATCY 6 cut(s) 161, 233, 908, 1768, 1892, 2378
BstZ17I GTATAC 3 cut(s) 131, 2102, 2499
BsuI GTATCC 2 cut(s) 662, 1321
BsuRI GGCC 5 cut(s) 46, 72, 366, 1319, 2966
BtgI CCRYGG 3 cut(s) 8, 697, 1632
BtgZI GCGATG 2 cut(s) 1651, 2178
BtrI CACGTC 3 cut(s) 736, 1172, 1231
BtsIMutI CAGTG 1 cut(s) 1254
BveI ACCTGC 2 cut(s) 2190, 2449
Cac8I GCNNGC 6 cut(s) 1222, 1236, 1688, 2446, 2512, 2766
CaiI CAGNNNCTG 1 cut(s) 3061
CciI TCATGA 1 cut(s) 2323
CfoI GCGC 4 cut(s) 1144, 1185, 2577, 2746
Cfr13I GGNCC 3 cut(s) 45, 70, 1318
Cfr42I CCGCGG 1 cut(s) 11
CseI GACGC 1 cut(s) 1919
Csp6I GTAC 9 cut(s) 442, 612, 743, 1335, 1722, 2147, 2191, 2453, 3115
CviQI GTAC 9 cut(s) 442, 612, 743, 1335, 1722, 2147, 2191, 2453, 3115
DdeI CTNAG 7 cut(s) 504, 1431, 1583, 1761, 2490, 2990, 3162
Eam1104I CTCTTC 7 cut(s) 65, 1117, 1755, 2013, 2037, 2156, 2922
EarI CTCTTC 7 cut(s) 65, 1117, 1755, 2013, 2037, 2156, 2922
EciI GGCGGA 1 cut(s) 1956
Ecl136II GAGCTC 3 cut(s) 265, 2764, 2848
Eco130I CCWWGG 5 cut(s) 646, 697, 1348, 1632, 2391
Eco147I AGGCCT 1 cut(s) 2966
Eco24I GRGCYC 3 cut(s) 267, 2766, 2850
Eco31I GGTCTC 2 cut(s) 349, 2142
Eco53kI GAGCTC 3 cut(s) 265, 2764, 2848
Eco57I CTGAAG 5 cut(s) 480, 1195, 1629, 1736, 2500
Eco88I CYCGRG 1 cut(s) 965
EcoICRI GAGCTC 3 cut(s) 265, 2764, 2848
EcoRI GAATTC 5 cut(s) 1360, 1418, 1818, 1953, 3019
EcoRII CCWGG 2 cut(s) 225, 2841
EcoT14I CCWWGG 5 cut(s) 646, 697, 1348, 1632, 2391
EcoT22I ATGCAT 2 cut(s) 2064, 2086
EcoT38I GRGCYC 3 cut(s) 267, 2766, 2850
ErhI CCWWGG 5 cut(s) 646, 697, 1348, 1632, 2391
FalI AAGNNNNNCTT 2 cut(s) 1389, 1421
FaqI GGGAC 3 cut(s) 596, 746, 2691
FauI CCCGC 1 cut(s) 3231
FauNDI CATATG 1 cut(s) 2221
FbaI TGATCA 2 cut(s) 673, 2884
FblI GTMKAC 3 cut(s) 130, 2101, 2498
FriOI GRGCYC 3 cut(s) 267, 2766, 2850
FspBI CTAG 9 cut(s) 311, 828, 1349, 1647, 2430, 2511, 2612, 2717, 2910
GlaI GCGC 4 cut(s) 1143, 1184, 2576, 2745
GsaI CCCAGC 1 cut(s) 659
HaeIII GGCC 5 cut(s) 46, 72, 366, 1319, 2966
HapII CCGG 3 cut(s) 468, 1392, 1873
HgaI GACGC 1 cut(s) 1919
HhaI GCGC 4 cut(s) 1144, 1185, 2577, 2746
Hin6I GCGC 4 cut(s) 1142, 1183, 2575, 2744
HinP1I GCGC 4 cut(s) 1142, 1183, 2575, 2744
HincII GTYRAC 6 cut(s) 223, 1030, 1453, 1489, 2878, 3144
HindII GTYRAC 6 cut(s) 223, 1030, 1453, 1489, 2878, 3144
HindIII AAGCTT 1 cut(s) 1556
HpaI GTTAAC 2 cut(s) 223, 3144
HpaII CCGG 3 cut(s) 468, 1392, 1873
HphI GGTGA 8 cut(s) 520, 817, 1823, 1985, 2045, 2112, 2197, 2430
Hpy99I CGWCG 2 cut(s) 325, 2794
HpyAV CCTTC 9 cut(s) 567, 1154, 1219, 1604, 1883, 2127, 2153, 2594, 3001
HpyCH4III ACNGT 8 cut(s) 135, 292, 616, 790, 805, 896, 1624, 3114
HpyCH4IV ACGT 6 cut(s) 302, 735, 1026, 1171, 1230, 2451
HpyF10VI GCNNNNNNNGC 7 cut(s) 78, 897, 1244, 1316, 2581, 2590, 3000
HpyF3I CTNAG 7 cut(s) 504, 1431, 1583, 1761, 2490, 2990, 3162
HpySE526I ACGT 6 cut(s) 302, 735, 1026, 1171, 1230, 2451
HspAI GCGC 4 cut(s) 1142, 1183, 2575, 2744
Ksp22I TGATCA 2 cut(s) 673, 2884
KspAI GTTAAC 2 cut(s) 223, 3144
KspI CCGCGG 1 cut(s) 11
LguI GCTCTTC 1 cut(s) 1755
LmnI GCTCC 4 cut(s) 962, 2620, 2845, 2930
Lsp1109I GCAGC 6 cut(s) 900, 1127, 1259, 1319, 2577, 3092
LweI GCATC 7 cut(s) 183, 1273, 1735, 2531, 2593, 2690, 3048
MaeI CTAG 9 cut(s) 311, 828, 1349, 1647, 2430, 2511, 2612, 2717, 2910
MaeII ACGT 6 cut(s) 302, 735, 1026, 1171, 1230, 2451
MbiI CCGCTC 1 cut(s) 555
MfeI CAATTG 1 cut(s) 1518
MflI RGATCY 6 cut(s) 161, 233, 908, 1768, 1892, 2378
MhlI GDGCHC 4 cut(s) 267, 1500, 2766, 2850
MlyI GAGTC 5 cut(s) 173, 193, 1289, 2919, 3172
MmeI TCCRAC 1 cut(s) 2256
Mph1103I ATGCAT 2 cut(s) 2064, 2086
MslI CAYNNNNRTG 2 cut(s) 2820, 3044
MspA1I CMGCKG 2 cut(s) 10, 2590
MspCI CTTAAG 1 cut(s) 2693
MspI CCGG 3 cut(s) 468, 1392, 1873
MspR9I CCNGG 4 cut(s) 227, 469, 1874, 2843
MunI CAATTG 1 cut(s) 1518
Mva1269I GAATGC 3 cut(s) 1056, 2086, 2345
MvaI CCWGG 2 cut(s) 227, 2843
MvnI CGCG 6 cut(s) 10, 392, 1144, 1686, 2575, 3140
MwoI GCNNNNNNNGC 7 cut(s) 78, 897, 1244, 1316, 2581, 2590, 3000
NciI CCSGG 2 cut(s) 469, 1874
NcoI CCATGG 2 cut(s) 697, 1632
NdeI CATATG 1 cut(s) 2221
NheI GCTAGC 1 cut(s) 2510
NlaIV GGNNCC 6 cut(s) 259, 484, 530, 937, 1638, 2115
NmuCI GTSAC 6 cut(s) 246, 839, 1165, 1508, 2033, 2837
NsiI ATGCAT 2 cut(s) 2064, 2086
NspI RCATGY 4 cut(s) 1459, 1533, 2019, 3150
NspV TTCGAA 3 cut(s) 138, 1848, 3130
PaeR7I CTCGAG 1 cut(s) 965
PagI TCATGA 1 cut(s) 2323
PaqCI CACCTGC 1 cut(s) 2449
PceI AGGCCT 1 cut(s) 2966
PciI ACATGT 4 cut(s) 1455, 1529, 2015, 3146
PciSI GCTCTTC 1 cut(s) 1755
PcsI WCGNNNNNNNCGW 2 cut(s) 809, 2934
PctI GAATGC 3 cut(s) 1056, 2086, 2345
Ple19I CGATCG 1 cut(s) 986
PleI GAGTC 5 cut(s) 173, 193, 1289, 2919, 3172
PpsI GAGTC 5 cut(s) 173, 193, 1289, 2919, 3172
PscI ACATGT 4 cut(s) 1455, 1529, 2015, 3146
PsiI TTATAA 2 cut(s) 608, 1070
Psp124BI GAGCTC 3 cut(s) 267, 2766, 2850
Psp1406I AACGTT 2 cut(s) 302, 1026
Psp6I CCWGG 2 cut(s) 225, 2841
PspFI CCCAGC 1 cut(s) 655
PspGI CCWGG 2 cut(s) 225, 2841
PspN4I GGNNCC 6 cut(s) 259, 484, 530, 937, 1638, 2115
PspPI GGNCC 3 cut(s) 45, 70, 1318
PsrI GAACNNNNNNTAC 2 cut(s) 114, 146
PstI CTGCAG 1 cut(s) 727
PstNI CAGNNNCTG 1 cut(s) 3061
PsuI RGATCY 6 cut(s) 161, 233, 908, 1768, 1892, 2378
PvuI CGATCG 1 cut(s) 986
RsaI GTAC 9 cut(s) 443, 613, 744, 1336, 1723, 2148, 2192, 2454, 3116
RsaNI GTAC 9 cut(s) 442, 612, 743, 1335, 1722, 2147, 2191, 2453, 3115
RseI CAYNNNNRTG 2 cut(s) 2820, 3044
SacI GAGCTC 3 cut(s) 267, 2766, 2850
SacII CCGCGG 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 1755
Sau96I GGNCC 3 cut(s) 45, 70, 1318
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 5 cut(s) 173, 193, 1289, 2919, 3172
ScrFI CCNGG 4 cut(s) 227, 469, 1874, 2843
SduI GDGCHC 4 cut(s) 267, 1500, 2766, 2850
SfaNI GCATC 7 cut(s) 183, 1273, 1735, 2531, 2593, 2690, 3048
SfcI CTRYAG 1 cut(s) 723
Sfr274I CTCGAG 1 cut(s) 965
Sfr303I CCGCGG 1 cut(s) 11
SfuI TTCGAA 3 cut(s) 138, 1848, 3130
SgrBI CCGCGG 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 965
SmiMI CAYNNNNRTG 2 cut(s) 2820, 3044
SpeI ACTAGT 2 cut(s) 827, 1646
SseBI AGGCCT 1 cut(s) 2966
SspMI CTAG 9 cut(s) 311, 828, 1349, 1647, 2430, 2511, 2612, 2717, 2910
SstI GAGCTC 3 cut(s) 267, 2766, 2850
StuI AGGCCT 1 cut(s) 2966
StyD4I CCNGG 4 cut(s) 225, 467, 1872, 2841
StyI CCWWGG 5 cut(s) 646, 697, 1348, 1632, 2391
TaaI ACNGT 8 cut(s) 135, 292, 616, 790, 805, 896, 1624, 3114
TaiI ACGT 6 cut(s) 305, 738, 1029, 1174, 1233, 2454
TatI WGTACW 5 cut(s) 611, 742, 1334, 1721, 2190
TauI GCSGC 4 cut(s) 13, 46, 539, 686
TscAI CASTG 1 cut(s) 1261
TseFI GTSAC 6 cut(s) 246, 839, 1165, 1508, 2033, 2837
TseI GCWGC 6 cut(s) 888, 1115, 1247, 1307, 2590, 3080
Tsp45I GTSAC 6 cut(s) 246, 839, 1165, 1508, 2033, 2837
TspGWI ACGGA 5 cut(s) 1354, 2490, 2667, 2871, 3112
TspRI CASTG 1 cut(s) 1261
Vha464I CTTAAG 1 cut(s) 2693
XapI RAATTY 7 cut(s) 1360, 1418, 1818, 1838, 1953, 2434, 3019
XbaI TCTAGA 1 cut(s) 2429
XceI RCATGY 4 cut(s) 1459, 1533, 2019, 3150
XhoI CTCGAG 1 cut(s) 965
XmaJI CCTAGG 1 cut(s) 1348
XmiI GTMKAC 3 cut(s) 130, 2101, 2498
XspI CTAG 9 cut(s) 311, 828, 1349, 1647, 2430, 2511, 2612, 2717, 2910
ZrmI AGTACT 1 cut(s) 613
Zsp2I ATGCAT 2 cut(s) 2064, 2086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.