pycom04g17180

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016705
Physical Location & Seq
Reverse (-)
19673666 .. 19676510
2845 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g17180.3

Sequence Viewer

Length: 2370 bp
ATGATCTCAATGTTGTTTAAGATGACAAAAAGAATCGAACATCGTATACTGTTCGAATCACATGAAGCACTCAACAGATCCTTTTCGAGCATCGACTCCACCTCAAAGTTGCTTACTCCATTGAATCCATCAGCAGTTAACCTGGCAAGATCTCTAACTTCGTCACTTAATGGAACCGAGCTCAATTTCACTACCATCCAAACAACGGTTGCGCCAACGTTGTTTCTAGCTCTTTCGACGATTCCACATATCTCACAGGTCTCTTACATCGGATTGGATGGCCTTTTATTCTCACTTAACAACTACGCGGATCAAACTTATGCAGTCTTCTCCAATGCTTCATTTTCTTCAAATTGGTACACTCAACCCATAAATAGAGACACGGGGAAATTATATGGCGCCGCAGTTGCTTCAGATTCTAAGGTGATAGTAAATGCAAGTTGGTTCCAAGCCGCCTTAAACAGCACAGGCGGTTATTCTTGGCTTGGAAGGGGATGGGACAAAGCTCAAGGTAGCTTATTCATGAGTACTGTGGTTATGGATGGCAAAGGTGTGATTTCCCTTGGTATCCCAGCAAAAGTTGTAGTTGATCATTTCGCGGCTTTAGACTTCCATGGGGGTTATTTTCACTTGGCTACTGCAGATGGGCATGTAATTGTACAATCAAAACTTCCGAAAACCCAATTTCAGATAAGCAACAACACGGTTTCGGTGCAAACGATGAGGCCAAATGGTAATATTACAAGTAATCTTGGTCACTTCCCATGTAATACTCCCGATGATGACGATGATGACAACGACAATGATGGCGGCAACGGTGACAAGTGGGGATCTTCTTACTCATGGAAACTCAAGGGAACCAAGTATAGGGCGTATTGCTCCACCCTCGAGATCATTGGAGTTCAATCGGTATATGTATTGACTTGTCCTGCCAATGGACTTGCTAACCTTGACCACAAATCGGTTGTGTTGCTTGGCATTCTGCTATTTATAATTCTTGTTTCTGTTTGCATATTCATATTCTTAATAATAAGAGCAGCAAGAAGAGAGATGATTTTATGTGCGCGGATCATAAAACAAGAAGGCGTGACACGTCAGGCAGAGCGCAAAAGTATGAACAAAACTAAAGCCTTTAGTAGAGCAAGCCATGACGTGCGTGCTTCATTAGCAGCCATCACTGGTTTAATAGAGTTGTGTCATCAAGATGCCAATCCTGACTCCGAATTAGCAGCCAACTTGGCCCAGATGGATACTTGTACAAATGACCTCCTAGGCATATTGAATTCCGTTCTTGATATAAGCAAGATTGAAGCCGGAAAAGTGCAACTTGAAATTGAAGAATTCAATCTGGCTCAGCTTCTTGAGGATGTAGTTGACATGTTCTATCCTATTGCTATCAAGAAAGGTGTTGACATCGTGCTCGATCCTTGTGACGATTCAATTGTCAAAACATGTAATGTGAGAGGCGACAGAGGAAAGCTTAAACAGATAATGTGCAACTTACTCAGCAATGCCGTGAAGTTTACATCTGAAGGTCATATCACAGTTCGTGCCATGGTTCCAAAAACTAGTTATGAAAATGAAATCATCGCTTCCAATCATAATCGCGTGCTGAATTGTTTGTCATGGCTCTTTTACAAGAGTACAGAAGACTTCAATGATTTGGATGCTCTTCATACATCTCAGAAAGATCCAAACAACACAAAGTTTGTCATTGAGGTCGATGATACAGGTGAGGGAATTCCTAGAGATAAGCGAGAATTTGTTTTCGAAAACTTTGTGCAAGTTACAGACCGGGCTACTGGAAAAGAAGGATCTGGTTTGGGACTTGGTATTGTTGAATCTTTGGTGCGTCTAATGGGCGGAGAAATAAGGATTCTTGATAAAGAGAGTGGTGAGAGAGGCACTTGTTTCAGCTTTGATGTTTCTCTTCTAACATGTAAACCCAAGTCTGGTGACATCGAAGAGGAATCCCGTATAATGCATAGTGATCGCTCTGCGAATGCATTTCAACCTTTTGGTATACAACTTTTGGTGCCTTCACCAAAACAAGAGGGGTCTCATGTCGTACTCTTCATAGAAGGCGATGAACGAAGAAAGATTTTGGTGAAGTACATCAGCAGGTTGAACATAAAAGTATCATATGTAAAGTATGCGAAAAATCTTCTTCCACAACTTGAGAAGATAAAGCGTAAGTTGGATATTTCCTACTTTAATTATTCAGAAAAATCCCAACAAGGTTTTCATGACCAATTAAGCACATCTGCGTTCAAAAACTCGGATTCAAGAGCAAGTGATGGATCGATCAAAGCACCAAGGATGAAGATGAACATACTTGTAGGAAGATCAACTCTAGAAATTCACCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HATPase_c HisKA Response_reg

Protein Analysis

790

Amino Acids

87.31

Weight (kDa)

6.67

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016705)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47430
malus_domestica MD04G1194300.v1.1
prunus_persica Prupe.6G314200_v2.0.a1
pyrus_communis pycom04g17180
rosa_chinensis RchiOBHm_Chr3g0456951
rosa_laevigata RLG00000025260
rosa_multiflora Rmu_sc0016008.1_g000003
rosa_roxburghii Rroxscaffold_6G00422340
rosa_rugosa Rorug03G0017600
rosa_samantha Rh3AG077600 Rh3BG079500 Rh3CG079400 Rh3DG081200
rosa_wichuraiana Rw3G006650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 992
Acc36I ACCTGC 1 cut(s) 2112
AccB1I GGYRCC 2 cut(s) 398, 2035
AccI GTMKAC 2 cut(s) 46, 2023
AccII CGCG 4 cut(s) 308, 599, 1066, 1608
AciI CCGC 8 cut(s) 308, 402, 453, 471, 599, 810, 1066, 1863
AclI AACGTT 1 cut(s) 218
AclWI GGATC 8 cut(s) 72, 318, 838, 1076, 1418, 1685, 1822, 2308
AcsI RAATTY 5 cut(s) 1282, 1340, 1740, 1760, 2358
AcuI CTGAAG 2 cut(s) 396, 1551
AcyI GRCGYC 1 cut(s) 399
AfaI GTAC 7 cut(s) 359, 529, 660, 1258, 1645, 2070, 2114
AfiI CCNNNNNNNGG 5 cut(s) 205, 867, 935, 961, 1952
AflIII ACRYGT 4 cut(s) 1091, 1377, 1451, 1937
AhlI ACTAGT 1 cut(s) 1568
AjiI CACGTC 2 cut(s) 1094, 1153
AjnI CCWGG 1 cut(s) 141
AjuI GAANNNNNNNTTGG 2 cut(s) 2226, 2258
AluBI AGCT 7 cut(s) 181, 230, 506, 516, 1357, 1480, 1917
AluI AGCT 7 cut(s) 181, 230, 506, 516, 1357, 1480, 1917
Alw21I GWGCWC 2 cut(s) 183, 1422
Alw26I GTCTC 3 cut(s) 265, 372, 2064
AlwI GGATC 8 cut(s) 72, 318, 838, 1076, 1418, 1685, 1822, 2308
Ama87I CYCGRG 1 cut(s) 887
AoxI GGCC 3 cut(s) 280, 725, 1239
ApeKI GCWGC 3 cut(s) 1037, 1169, 1229
ApoI RAATTY 5 cut(s) 1282, 1340, 1740, 1760, 2358
AspA2I CCTAGG 1 cut(s) 1270
AspLEI GCGC 4 cut(s) 214, 401, 1066, 1107
AspS9I GGNCC 1 cut(s) 1240
AsuC2I CCSGG 1 cut(s) 1796
AsuHPI GGTGA 8 cut(s) 436, 830, 1745, 1907, 1967, 2034, 2119, 2354
AsuII TTCGAA 2 cut(s) 54, 1770
AvaI CYCGRG 1 cut(s) 887
AvrII CCTAGG 1 cut(s) 1270
BanI GGYRCC 2 cut(s) 398, 2035
BanII GRGCYC 1 cut(s) 183
BbsI GAAGAC 2 cut(s) 319, 1656
Bbv12I GWGCWC 2 cut(s) 183, 1422
BbvI GCAGC 3 cut(s) 1049, 1181, 1241
BceAI ACGGC 1 cut(s) 1499
BcgI CGANNNNNNTGC 2 cut(s) 1973, 2007
BciT130I CCWGG 1 cut(s) 143
BciVI GTATCC 2 cut(s) 578, 1243
BclI TGATCA 1 cut(s) 589
BcnI CCSGG 1 cut(s) 1796
BcoDI GTCTC 3 cut(s) 265, 372, 2064
BcuI ACTAGT 1 cut(s) 1568
BfaI CTAG 5 cut(s) 227, 1271, 1569, 1746, 2354
BfmI CTRYAG 1 cut(s) 639
BfoI RGCGCY 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 2112
BfuI GTATCC 2 cut(s) 578, 1243
BglI GCCNNNNNGGC 1 cut(s) 1238
BglII AGATCT 1 cut(s) 149
BisI GCNGC 7 cut(s) 402, 453, 600, 811, 1038, 1170, 1230
BlnI CCTAGG 1 cut(s) 1270
BlpI GCTNAGC 1 cut(s) 1353
BlsI GCNGC 7 cut(s) 403, 454, 601, 812, 1039, 1171, 1231
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 2 cut(s) 143, 1796
BmeT110I CYCGRG 1 cut(s) 887
BmgBI CACGTC 2 cut(s) 1094, 1153
BmgT120I GGNCC 1 cut(s) 1240
BmiI GGNNCC 6 cut(s) 175, 400, 446, 859, 1560, 2037
BmrFI CCNGG 2 cut(s) 143, 1796
BmsI GCATC 3 cut(s) 99, 1195, 1657
BpiI GAAGAC 2 cut(s) 319, 1656
Bpu1102I GCTNAGC 1 cut(s) 1353
Bpu14I TTCGAA 2 cut(s) 54, 1770
BpuEI CTTGAG 4 cut(s) 492, 836, 1382, 2198
BpuMI CCSGG 1 cut(s) 1796
Bsa29I ATCGAT 1 cut(s) 2303
BsaBI GATNNNNATC 1 cut(s) 1209
BsaHI GRCGYC 1 cut(s) 399
BsaI GGTCTC 2 cut(s) 265, 2064
BsaJI CCNNGG 5 cut(s) 562, 613, 1270, 1554, 2315
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 867, 935, 961, 1952
Bse1I ACTGG 2 cut(s) 1183, 1807
Bse3DI GCAATG 1 cut(s) 1516
Bse8I GATNNNNATC 1 cut(s) 1209
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 2303
BseDI CCNNGG 5 cut(s) 562, 613, 1270, 1554, 2315
BseGI GGATG 7 cut(s) 195, 283, 500, 547, 1372, 1672, 2325
BseJI GATNNNNATC 1 cut(s) 1209
BseLI CCNNNNNNNGG 5 cut(s) 205, 867, 935, 961, 1952
BseMI GCAATG 1 cut(s) 1516
BseMII CTCAG 3 cut(s) 1367, 1519, 1697
BseNI ACTGG 2 cut(s) 1183, 1807
BseXI GCAGC 3 cut(s) 1049, 1181, 1241
BseYI CCCAGC 1 cut(s) 571
Bsh1236I CGCG 4 cut(s) 308, 599, 1066, 1608
BshFI GGCC 3 cut(s) 282, 727, 1241
BshNI GGYRCC 2 cut(s) 398, 2035
BshVI ATCGAT 1 cut(s) 2303
BsiHKAI GWGCWC 2 cut(s) 183, 1422
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 2 cut(s) 1314, 1795
BslFI GGGAC 2 cut(s) 512, 1839
BslI CCNNNNNNNGG 5 cut(s) 205, 867, 935, 961, 1952
BsmAI GTCTC 3 cut(s) 265, 372, 2064
BsmFI GGGAC 2 cut(s) 512, 1839
BsmI GAATGC 2 cut(s) 978, 2008
BsnI GGCC 3 cut(s) 282, 727, 1241
Bso31I GGTCTC 2 cut(s) 265, 2064
BsoBI CYCGRG 1 cut(s) 887
Bsp119I TTCGAA 2 cut(s) 54, 1770
Bsp1286I GDGCHC 2 cut(s) 183, 1422
Bsp1407I TGTACA 2 cut(s) 658, 1256
Bsp1720I GCTNAGC 1 cut(s) 1353
Bsp19I CCATGG 2 cut(s) 613, 1554
BspACI CCGC 8 cut(s) 308, 402, 453, 471, 599, 810, 1066, 1863
BspANI GGCC 3 cut(s) 282, 727, 1241
BspCNI CTCAG 3 cut(s) 1366, 1518, 1696
BspDI ATCGAT 1 cut(s) 2303
BspFNI CGCG 4 cut(s) 308, 599, 1066, 1608
BspHI TCATGA 2 cut(s) 522, 2245
BspLI GGNNCC 6 cut(s) 175, 400, 446, 859, 1560, 2037
BspMAI CTGCAG 1 cut(s) 643
BspMI ACCTGC 1 cut(s) 2112
BspPI GGATC 8 cut(s) 72, 318, 838, 1076, 1418, 1685, 1822, 2308
BspQI GCTCTTC 1 cut(s) 1677
BspT104I TTCGAA 2 cut(s) 54, 1770
BspT107I GGYRCC 2 cut(s) 398, 2035
BspTNI GGTCTC 2 cut(s) 265, 2064
BsrDI GCAATG 1 cut(s) 1516
BsrGI TGTACA 2 cut(s) 658, 1256
BsrI ACTGG 2 cut(s) 1183, 1807
BssECI CCNNGG 5 cut(s) 562, 613, 1270, 1554, 2315
BssNAI GTATAC 2 cut(s) 47, 2024
BssNI GRCGYC 1 cut(s) 399
BssT1I CCWWGG 5 cut(s) 562, 613, 1270, 1554, 2315
Bst1107I GTATAC 2 cut(s) 47, 2024
Bst2UI CCWGG 1 cut(s) 143
Bst4CI ACNGT 6 cut(s) 51, 208, 532, 706, 818, 1546
Bst6I CTCTTC 5 cut(s) 1039, 1677, 1935, 1959, 2078
BstACI GRCGYC 1 cut(s) 399
BstAUI TGTACA 2 cut(s) 658, 1256
BstBI TTCGAA 2 cut(s) 54, 1770
BstC8I GCNNGC 3 cut(s) 1144, 1158, 1610
BstDEI CTNAG 4 cut(s) 420, 1353, 1505, 1683
BstDSI CCRYGG 2 cut(s) 613, 1554
BstF5I GGATG 7 cut(s) 195, 283, 500, 547, 1372, 1672, 2325
BstFNI CGCG 4 cut(s) 308, 599, 1066, 1608
BstH2I RGCGCY 1 cut(s) 402
BstHHI GCGC 4 cut(s) 214, 401, 1066, 1107
BstMAI GTCTC 3 cut(s) 265, 372, 2064
BstMWI GCNNNNNNNGC 3 cut(s) 407, 1166, 1238
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 4 cut(s) 653, 1381, 1455, 1941
BstSCI CCNGG 2 cut(s) 141, 1794
BstSFI CTRYAG 1 cut(s) 639
BstUI CGCG 4 cut(s) 308, 599, 1066, 1608
BstV1I GCAGC 3 cut(s) 1049, 1181, 1241
BstV2I GAAGAC 2 cut(s) 319, 1656
BstX2I RGATCY 5 cut(s) 77, 149, 830, 1690, 1814
BstYI RGATCY 5 cut(s) 77, 149, 830, 1690, 1814
BstZ17I GTATAC 2 cut(s) 47, 2024
Bsu15I ATCGAT 1 cut(s) 2303
BsuI GTATCC 2 cut(s) 578, 1243
BsuRI GGCC 3 cut(s) 282, 727, 1241
BsuTUI ATCGAT 1 cut(s) 2303
BtgI CCRYGG 2 cut(s) 613, 1554
BtgZI GCGATG 2 cut(s) 1573, 2100
BtrI CACGTC 2 cut(s) 1094, 1153
BtsCI GGATG 7 cut(s) 195, 283, 500, 547, 1372, 1672, 2325
BtsIMutI CAGTG 1 cut(s) 1176
BveI ACCTGC 1 cut(s) 2112
Cac8I GCNNGC 3 cut(s) 1144, 1158, 1610
CciI TCATGA 2 cut(s) 522, 2245
CfoI GCGC 4 cut(s) 214, 401, 1066, 1107
Cfr13I GGNCC 1 cut(s) 1240
ClaI ATCGAT 1 cut(s) 2303
CseI GACGC 1 cut(s) 1841
Csp6I GTAC 7 cut(s) 358, 528, 659, 1257, 1644, 2069, 2113
CviQI GTAC 7 cut(s) 358, 528, 659, 1257, 1644, 2069, 2113
DdeI CTNAG 4 cut(s) 420, 1353, 1505, 1683
DinI GGCGCC 1 cut(s) 400
Eam1104I CTCTTC 5 cut(s) 1039, 1677, 1935, 1959, 2078
EarI CTCTTC 5 cut(s) 1039, 1677, 1935, 1959, 2078
EciI GGCGGA 1 cut(s) 1878
Ecl136II GAGCTC 1 cut(s) 181
Eco130I CCWWGG 5 cut(s) 562, 613, 1270, 1554, 2315
Eco24I GRGCYC 1 cut(s) 183
Eco31I GGTCTC 2 cut(s) 265, 2064
Eco53kI GAGCTC 1 cut(s) 181
Eco57I CTGAAG 2 cut(s) 396, 1551
Eco88I CYCGRG 1 cut(s) 887
EcoICRI GAGCTC 1 cut(s) 181
EcoRI GAATTC 3 cut(s) 1282, 1340, 1740
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 5 cut(s) 562, 613, 1270, 1554, 2315
EcoT22I ATGCAT 2 cut(s) 1986, 2008
EcoT38I GRGCYC 1 cut(s) 183
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
ErhI CCWWGG 5 cut(s) 562, 613, 1270, 1554, 2315
FalI AAGNNNNNCTT 2 cut(s) 1311, 1343
FaqI GGGAC 2 cut(s) 512, 1839
FauNDI CATATG 1 cut(s) 2143
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 2 cut(s) 46, 2023
Fnu4HI GCNGC 7 cut(s) 402, 453, 600, 811, 1038, 1170, 1230
FokI GGATG 7 cut(s) 182, 290, 507, 554, 1379, 1679, 2332
FriOI GRGCYC 1 cut(s) 183
Fsp4HI GCNGC 7 cut(s) 402, 453, 600, 811, 1038, 1170, 1230
FspBI CTAG 5 cut(s) 227, 1271, 1569, 1746, 2354
GlaI GCGC 4 cut(s) 213, 400, 1065, 1106
GluI GCNGC 7 cut(s) 402, 453, 600, 811, 1038, 1170, 1230
GsaI CCCAGC 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 402
HaeIII GGCC 3 cut(s) 282, 727, 1241
HapII CCGG 2 cut(s) 1314, 1795
HgaI GACGC 1 cut(s) 1841
HhaI GCGC 4 cut(s) 214, 401, 1066, 1107
Hin1I GRCGYC 1 cut(s) 399
Hin6I GCGC 4 cut(s) 212, 399, 1064, 1105
HinP1I GCGC 4 cut(s) 212, 399, 1064, 1105
HincII GTYRAC 3 cut(s) 139, 1375, 1411
HindII GTYRAC 3 cut(s) 139, 1375, 1411
HindIII AAGCTT 1 cut(s) 1478
HpaI GTTAAC 1 cut(s) 139
HpaII CCGG 2 cut(s) 1314, 1795
HphI GGTGA 8 cut(s) 436, 830, 1745, 1907, 1967, 2034, 2119, 2354
Hpy166II GTNNAC 8 cut(s) 47, 139, 360, 1375, 1411, 1524, 1943, 2024
Hpy188I TCNGA 9 cut(s) 272, 415, 675, 690, 1222, 1531, 1686, 2224, 2281
Hpy8I GTNNAC 8 cut(s) 47, 139, 360, 1375, 1411, 1524, 1943, 2024
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 6 cut(s) 483, 1076, 1526, 1805, 2049, 2075
HpyCH4III ACNGT 6 cut(s) 51, 208, 532, 706, 818, 1546
HpyCH4IV ACGT 3 cut(s) 218, 1093, 1152
HpyF10VI GCNNNNNNNGC 3 cut(s) 407, 1166, 1238
HpyF3I CTNAG 4 cut(s) 420, 1353, 1505, 1683
HpySE526I ACGT 3 cut(s) 218, 1093, 1152
Hsp92I GRCGYC 1 cut(s) 399
HspAI GCGC 4 cut(s) 212, 399, 1064, 1105
KasI GGCGCC 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 589
KspAI GTTAAC 1 cut(s) 139
LguI GCTCTTC 1 cut(s) 1677
LmnI GCTCC 1 cut(s) 884
Lsp1109I GCAGC 3 cut(s) 1049, 1181, 1241
LweI GCATC 3 cut(s) 99, 1195, 1657
MaeI CTAG 5 cut(s) 227, 1271, 1569, 1746, 2354
MaeII ACGT 3 cut(s) 218, 1093, 1152
MaeIII GTNAC 7 cut(s) 162, 755, 818, 1087, 1430, 1786, 1955
MfeI CAATTG 1 cut(s) 1440
MflI RGATCY 5 cut(s) 77, 149, 830, 1690, 1814
MhlI GDGCHC 2 cut(s) 183, 1422
Mly113I GGCGCC 1 cut(s) 399
MlyI GAGTC 2 cut(s) 89, 1211
MmeI TCCRAC 1 cut(s) 2178
Mph1103I ATGCAT 2 cut(s) 1986, 2008
MslI CAYNNNNRTG 1 cut(s) 1203
MspI CCGG 2 cut(s) 1314, 1795
MspR9I CCNGG 2 cut(s) 143, 1796
MunI CAATTG 1 cut(s) 1440
Mva1269I GAATGC 2 cut(s) 978, 2008
MvaI CCWGG 1 cut(s) 143
MvnI CGCG 4 cut(s) 308, 599, 1066, 1608
MwoI GCNNNNNNNGC 3 cut(s) 407, 1166, 1238
NarI GGCGCC 1 cut(s) 399
NciI CCSGG 1 cut(s) 1796
NcoI CCATGG 2 cut(s) 613, 1554
NdeI CATATG 1 cut(s) 2143
NlaIV GGNNCC 6 cut(s) 175, 400, 446, 859, 1560, 2037
NmuCI GTSAC 6 cut(s) 162, 755, 818, 1087, 1430, 1955
NsiI ATGCAT 2 cut(s) 1986, 2008
NspI RCATGY 4 cut(s) 653, 1381, 1455, 1941
NspV TTCGAA 2 cut(s) 54, 1770
PaeR7I CTCGAG 1 cut(s) 887
PagI TCATGA 2 cut(s) 522, 2245
PciI ACATGT 3 cut(s) 1377, 1451, 1937
PciSI GCTCTTC 1 cut(s) 1677
PcsI WCGNNNNNNNCGW 1 cut(s) 716
PctI GAATGC 2 cut(s) 978, 2008
PkrI GCNGC 7 cut(s) 403, 454, 601, 812, 1039, 1171, 1231
PleI GAGTC 2 cut(s) 89, 1211
PluTI GGCGCC 1 cut(s) 402
PpsI GAGTC 2 cut(s) 89, 1211
PscI ACATGT 3 cut(s) 1377, 1451, 1937
PsiI TTATAA 1 cut(s) 992
Psp124BI GAGCTC 1 cut(s) 183
Psp1406I AACGTT 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 141
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 6 cut(s) 175, 400, 446, 859, 1560, 2037
PspPI GGNCC 1 cut(s) 1240
PsrI GAACNNNNNNTAC 2 cut(s) 30, 62
PstI CTGCAG 1 cut(s) 643
PsuI RGATCY 5 cut(s) 77, 149, 830, 1690, 1814
RsaI GTAC 7 cut(s) 359, 529, 660, 1258, 1645, 2070, 2114
RsaNI GTAC 7 cut(s) 358, 528, 659, 1257, 1644, 2069, 2113
RseI CAYNNNNRTG 1 cut(s) 1203
SacI GAGCTC 1 cut(s) 183
SapI GCTCTTC 1 cut(s) 1677
SatI GCNGC 7 cut(s) 402, 453, 600, 811, 1038, 1170, 1230
Sau96I GGNCC 1 cut(s) 1240
ScaI AGTACT 1 cut(s) 529
SchI GAGTC 2 cut(s) 89, 1211
ScrFI CCNGG 2 cut(s) 143, 1796
SduI GDGCHC 2 cut(s) 183, 1422
SfaNI GCATC 3 cut(s) 99, 1195, 1657
SfcI CTRYAG 1 cut(s) 639
SfoI GGCGCC 1 cut(s) 400
Sfr274I CTCGAG 1 cut(s) 887
SfuI TTCGAA 2 cut(s) 54, 1770
SlaI CTCGAG 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 1203
SmlI CTYRAG 5 cut(s) 507, 851, 887, 1361, 2177
SmoI CTYRAG 5 cut(s) 507, 851, 887, 1361, 2177
SpeI ACTAGT 1 cut(s) 1568
SsiI CCGC 8 cut(s) 308, 402, 453, 471, 599, 810, 1066, 1863
SspDI GGCGCC 1 cut(s) 398
SspI AATATT 1 cut(s) 739
SspMI CTAG 5 cut(s) 227, 1271, 1569, 1746, 2354
SstI GAGCTC 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 141, 1794
StyI CCWWGG 5 cut(s) 562, 613, 1270, 1554, 2315
TaaI ACNGT 6 cut(s) 51, 208, 532, 706, 818, 1546
TaiI ACGT 3 cut(s) 221, 1096, 1155
TatI WGTACW 5 cut(s) 527, 658, 1256, 1643, 2112
TauI GCSGC 4 cut(s) 404, 455, 602, 813
TscAI CASTG 1 cut(s) 1183
TseFI GTSAC 6 cut(s) 162, 755, 818, 1087, 1430, 1955
TseI GCWGC 3 cut(s) 1037, 1169, 1229
Tsp45I GTSAC 6 cut(s) 162, 755, 818, 1087, 1430, 1955
TspGWI ACGGA 1 cut(s) 1276
TspRI CASTG 1 cut(s) 1183
XapI RAATTY 5 cut(s) 1282, 1340, 1740, 1760, 2358
XbaI TCTAGA 1 cut(s) 2353
XceI RCATGY 4 cut(s) 653, 1381, 1455, 1941
XhoI CTCGAG 1 cut(s) 887
XmaJI CCTAGG 1 cut(s) 1270
XmiI GTMKAC 2 cut(s) 46, 2023
XspI CTAG 5 cut(s) 227, 1271, 1569, 1746, 2354
ZrmI AGTACT 1 cut(s) 529
Zsp2I ATGCAT 2 cut(s) 1986, 2008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.