MD04G1195800.v1.1

Calmodulin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007733
Physical Location & Seq
Forward (+)
28434208 .. 28438025
3818 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1195800.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGAACTTCAAGAGGCATCTTAGTGGCGAGGATGGCAAGGAGTTTGAATTTCCGGTTCCAGAACCGAAAAGGAGACTTACTTTTACCAATGTTCTTAGGGATGCGATGACGGATGCCAGACTGAATGGCGTGTTGGAGCGCTCCTTTCAAAGAATAGTGCGAGATGAGTTGGAGCGACGTATTCTTCCCTCCTTCATGGCATCTCCAAGGCCCTCAAACGAAAGTGGGAGTACATCTGGAGGAAGCGGCTTGCAGTTGCGTTTTATCAACAAGCTGCCAACTACCATATTCACAGGCAGTAGGGTGGAAGCAGAGAACCGCGAGCCTCTTCAAATTGAACTGGTTGATGCCAGTACTGGTGCTATAGTCCATTCCGGTCCCCTATCTTCACTAAAGGTTGAACTTTTTGTGCTGAATGGTGAGTTTGGGTCTGATGATCAAGAGGATTGGACCGAAAAAGAACTCAACACCTACATTGTGCGCGAAAGGGAAGGTAAAAGGCCATTAGTGACGGGTGACATACATGTTACACTGAGAGAGGGAGTTGGTTCTCTCGGTGATGTCATGTTTACCGACAACTCAAGCTGGGTAAGATGCAGAAAGTTCAGACTAGCAGCTCGAGTTGTGGCCAAAGCCCAGAGTGAAGTTCGAATTAGGGAAGCTACAAGTAAACCGTTTGTGGTTAAAGATCACCGCGGAGAACTGTATAAGAAACACCACCCTCCACACCCAAACGACGAAATATGGCGCCTGGAAAAGATATCGAAAGATGGTGCCTCTCATAAGAGACTGTACGAGAAGGGAATCAGCACCGTGGAGGACTTCCTGCAGTCGTACATGAAAGATGCATCCTCGCTACGAAATGTTTTGCGTGGGATCTCGAACAAGATATGGGATACAATCGTACAGCATGCAATGGCCTGTAAGTTGGATGATCGTAAGTTCTATGCCTACCACATAGCTGAGCAGGACGTAAGTCTCATGTTCAATTCCATCCACGGGCTTGAAGGGGCAATAATCAATGGCCAGTTCTGTTCTCTGTATGAACTCGACTCGAATCAGAAGATGCTGGTGGAAGCTTTGAAGCAGCAGGCTTATCGAAATGTAAGAGATCTGGTGCCCATTGACGCTTCGACTATGTTTGGCCTTTCCAAGCTTTTGCCAGTTCTACAAGCTGATTCATTCACTTGTCCAAACCCTGACCTGCGGCAAAGTGAATTTCAGTTAACACATCAAGATGGACTCCCAATACAACTAGGTTTCAACCATGCATCAAATTCAACCTCATGTCCCTACCAAGCAGAAGGTAGCAGCAATCAGCTAGTGGTTTCTGTGGCACAACCTAGCGAGCCAATACATCTCCGAAGCAGCAGCTTTTCGATGGAGGATCTTAGCTCCATTATCGACAACGGAGAAAGCAGTTGGCCGCCTGTCGGTGGTTTTCAAGCGCCAATTGTCCCAACTGGTCATTTAGGCACAGAATGTAAAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0002229 GO:0002237 GO:0002239 GO:0002376 GO:0002682 GO:0002831 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006725 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009696 GO:0009697 GO:0009787 GO:0009789 GO:0009814 GO:0009816 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010646 GO:0010647 GO:0010817 GO:0016053 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0031347 GO:0032101 GO:0032350 GO:0032787 GO:0033554 GO:0036293 GO:0036294 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0043900 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0046189 GO:0046394 GO:0046885 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070482 GO:0070887 GO:0071216 GO:0071219 GO:0071453 GO:0071456 GO:0071704 GO:0072330 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901421 GO:1901576 GO:1901615 GO:1901617 GO:1902584 GO:1903506 GO:1905957 GO:1905959 GO:2000070 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

498

Amino Acids

55.75

Weight (kDa)

6.23

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 84 - 231 3.1e-60 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 244 - 308 1e-26 Calmodulin binding protein central domain
Calmod_bind_C PF20452 314 - 374 8.3e-17 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1212
AccB1I GGYRCC 3 cut(s) 747, 773, 1117
AccII CGCG 3 cut(s) 321, 483, 696
AciI CCGC 6 cut(s) 246, 319, 694, 696, 1207, 1427
AclWI GGATC 2 cut(s) 884, 1395
AcoI YGGCCR 3 cut(s) 627, 1024, 1424
AcsI RAATTY 3 cut(s) 47, 1217, 1276
AcyI GRCGYC 1 cut(s) 748
AfaI GTAC 5 cut(s) 232, 355, 794, 836, 906
AfeI AGCGCT 1 cut(s) 140
AfiI CCNNNNNNNGG 3 cut(s) 999, 1433, 1436
AflIII ACRYGT 1 cut(s) 523
AjnI CCWGG 1 cut(s) 750
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
Alw26I GTCTC 3 cut(s) 67, 781, 983
AlwI GGATC 2 cut(s) 884, 1395
Ama87I CYCGRG 1 cut(s) 618
Aor51HI AGCGCT 1 cut(s) 140
AoxI GGCC 7 cut(s) 209, 500, 627, 918, 1024, 1144, 1424
ApeKI GCWGC 6 cut(s) 274, 614, 1087, 1311, 1368, 1371
ApoI RAATTY 3 cut(s) 47, 1217, 1276
AspLEI GCGC 4 cut(s) 141, 483, 750, 1450
AspS9I GGNCC 3 cut(s) 210, 377, 450
AsuHPI GGTGA 4 cut(s) 431, 527, 569, 683
AsuII TTCGAA 1 cut(s) 649
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 2 cut(s) 377, 450
BaeGI GKGCMC 1 cut(s) 1122
BaeI ACNNNNGTAYC 2 cut(s) 888, 921
BalI TGGCCA 2 cut(s) 629, 1026
BanI GGYRCC 3 cut(s) 747, 773, 1117
BbvI GCAGC 6 cut(s) 261, 626, 1099, 1323, 1380, 1383
BccI CCATC 5 cut(s) 26, 764, 1001, 1232, 1375
BcgI CGANNNNNNTGC 2 cut(s) 1079, 1113
BciT130I CCWGG 1 cut(s) 752
BciVI GTATCC 1 cut(s) 889
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 3 cut(s) 67, 781, 983
BfaI CTAG 4 cut(s) 611, 1256, 1322, 1344
BfmI CTRYAG 2 cut(s) 363, 827
BfoI RGCGCY 3 cut(s) 142, 751, 1451
BfuAI ACCTGC 1 cut(s) 1212
BfuI GTATCC 1 cut(s) 889
BglII AGATCT 1 cut(s) 1111
BisI GCNGC 9 cut(s) 247, 275, 615, 1088, 1208, 1312, 1369, 1372, 1427
BlpI GCTNAGC 1 cut(s) 963
BlsI GCNGC 9 cut(s) 248, 276, 616, 1089, 1209, 1313, 1370, 1373, 1428
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 2 cut(s) 377, 450
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 3 cut(s) 210, 377, 450
BmiI GGNNCC 5 cut(s) 57, 379, 749, 775, 1119
BmrFI CCNGG 1 cut(s) 752
BoxI GACNNNNGTC 1 cut(s) 975
BpmI CTGGAG 1 cut(s) 258
Bpu1102I GCTNAGC 1 cut(s) 963
Bpu14I TTCGAA 1 cut(s) 649
BpuEI CTTGAG 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 748
BsaJI CCNNGG 4 cut(s) 206, 694, 813, 997
BsaWI WCCGGW 2 cut(s) 52, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 999, 1433, 1436
Bse1I ACTGG 6 cut(s) 345, 351, 361, 1027, 1163, 1468
Bse3DI GCAATG 1 cut(s) 921
BseBI CCWGG 1 cut(s) 752
BseDI CCNNGG 4 cut(s) 206, 694, 813, 997
BseGI GGATG 6 cut(s) 37, 106, 118, 848, 937, 993
BseLI CCNNNNNNNGG 3 cut(s) 999, 1433, 1436
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 2 cut(s) 524, 954
BseNI ACTGG 6 cut(s) 345, 351, 361, 1027, 1163, 1468
BseSI GKGCMC 1 cut(s) 1122
BseXI GCAGC 6 cut(s) 261, 626, 1099, 1323, 1380, 1383
BseYI CCCAGC 1 cut(s) 585
Bsh1236I CGCG 3 cut(s) 321, 483, 696
BshFI GGCC 7 cut(s) 211, 502, 629, 920, 1026, 1146, 1426
BshNI GGYRCC 3 cut(s) 747, 773, 1117
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 2 cut(s) 53, 375
BslFI GGGAC 3 cut(s) 363, 1275, 1442
BslI CCNNNNNNNGG 3 cut(s) 999, 1433, 1436
BsmAI GTCTC 3 cut(s) 67, 781, 983
BsmFI GGGAC 3 cut(s) 363, 1275, 1442
BsnI GGCC 7 cut(s) 211, 502, 629, 920, 1026, 1146, 1426
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 649
Bsp1286I GDGCHC 1 cut(s) 1122
Bsp143I GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
Bsp1720I GCTNAGC 1 cut(s) 963
BspACI CCGC 6 cut(s) 246, 319, 694, 696, 1207, 1427
BspANI GGCC 7 cut(s) 211, 502, 629, 920, 1026, 1146, 1426
BspCNI CTCAG 2 cut(s) 525, 955
BspFNI CGCG 3 cut(s) 321, 483, 696
BspLI GGNNCC 5 cut(s) 57, 379, 749, 775, 1119
BspMAI CTGCAG 1 cut(s) 831
BspMI ACCTGC 1 cut(s) 1212
BspPI GGATC 2 cut(s) 884, 1395
BspT104I TTCGAA 1 cut(s) 649
BspT107I GGYRCC 3 cut(s) 747, 773, 1117
BsrDI GCAATG 1 cut(s) 921
BsrI ACTGG 6 cut(s) 345, 351, 361, 1027, 1163, 1468
BssECI CCNNGG 4 cut(s) 206, 694, 813, 997
BssMI GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
BssNI GRCGYC 1 cut(s) 748
BssT1I CCWWGG 1 cut(s) 206
Bst2UI CCWGG 1 cut(s) 752
Bst4CI ACNGT 4 cut(s) 675, 705, 792, 814
Bst6I CTCTTC 1 cut(s) 333
BstACI GRCGYC 1 cut(s) 748
BstBI TTCGAA 1 cut(s) 649
BstC8I GCNNGC 5 cut(s) 251, 323, 912, 1092, 1349
BstDEI CTNAG 6 cut(s) 20, 95, 533, 963, 1391, 1491
BstDSI CCRYGG 3 cut(s) 694, 813, 997
BstF5I GGATG 6 cut(s) 37, 106, 118, 848, 937, 993
BstFNI CGCG 3 cut(s) 321, 483, 696
BstH2I RGCGCY 3 cut(s) 142, 751, 1451
BstHHI GCGC 4 cut(s) 141, 483, 750, 1450
BstKTI GATC 6 cut(s) 439, 691, 879, 937, 1114, 1390
BstMAI GTCTC 3 cut(s) 67, 781, 983
BstMBI GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 752
BstNSI RCATGY 2 cut(s) 527, 914
BstPAI GACNNNNGTC 1 cut(s) 975
BstSCI CCNGG 1 cut(s) 750
BstSFI CTRYAG 2 cut(s) 363, 827
BstSLI GKGCMC 1 cut(s) 1122
BstUI CGCG 3 cut(s) 321, 483, 696
BstV1I GCAGC 6 cut(s) 261, 626, 1099, 1323, 1380, 1383
BstX2I RGATCY 3 cut(s) 876, 1111, 1387
BstYI RGATCY 3 cut(s) 876, 1111, 1387
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 7 cut(s) 211, 502, 629, 920, 1026, 1146, 1426
BtgI CCRYGG 3 cut(s) 694, 813, 997
BtgZI GCGATG 1 cut(s) 119
BtsCI GGATG 6 cut(s) 37, 106, 118, 848, 937, 993
BtsIMutI CAGTG 1 cut(s) 530
BveI ACCTGC 1 cut(s) 1212
Cac8I GCNNGC 5 cut(s) 251, 323, 912, 1092, 1349
CfoI GCGC 4 cut(s) 141, 483, 750, 1450
Cfr13I GGNCC 3 cut(s) 210, 377, 450
Cfr42I CCGCGG 1 cut(s) 697
CseI GACGC 1 cut(s) 1136
Csp6I GTAC 5 cut(s) 231, 354, 793, 835, 905
CviAII CATG 8 cut(s) 196, 524, 565, 838, 911, 982, 1268, 1287
CviQI GTAC 5 cut(s) 231, 354, 793, 835, 905
DdeI CTNAG 6 cut(s) 20, 95, 533, 963, 1391, 1491
DinI GGCGCC 1 cut(s) 749
DpnI GATC 6 cut(s) 438, 690, 878, 936, 1113, 1389
DpnII GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
EaeI YGGCCR 3 cut(s) 627, 1024, 1424
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 206
Eco32I GATATC 1 cut(s) 762
Eco47I GGWCC 2 cut(s) 377, 450
Eco47III AGCGCT 1 cut(s) 140
Eco88I CYCGRG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 210
EcoRII CCWGG 1 cut(s) 750
EcoRV GATATC 1 cut(s) 762
EcoT14I CCWWGG 1 cut(s) 206
EcoT22I ATGCAT 2 cut(s) 850, 1273
EgeI GGCGCC 1 cut(s) 749
EheI GGCGCC 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 206
FaeI CATG 8 cut(s) 199, 527, 568, 841, 914, 985, 1271, 1290
FaqI GGGAC 3 cut(s) 363, 1275, 1442
FatI CATG 8 cut(s) 195, 523, 564, 837, 910, 981, 1267, 1286
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 9 cut(s) 247, 275, 615, 1088, 1208, 1312, 1369, 1372, 1427
FokI GGATG 6 cut(s) 44, 113, 125, 835, 944, 980
Fsp4HI GCNGC 9 cut(s) 247, 275, 615, 1088, 1208, 1312, 1369, 1372, 1427
FspBI CTAG 4 cut(s) 611, 1256, 1322, 1344
GlaI GCGC 4 cut(s) 140, 482, 749, 1449
GluI GCNGC 9 cut(s) 247, 275, 615, 1088, 1208, 1312, 1369, 1372, 1427
GsaI CCCAGC 1 cut(s) 589
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 3 cut(s) 142, 751, 1451
HaeIII GGCC 7 cut(s) 211, 502, 629, 920, 1026, 1146, 1426
HapII CCGG 2 cut(s) 53, 375
HgaI GACGC 1 cut(s) 1136
HhaI GCGC 4 cut(s) 141, 483, 750, 1450
Hin1I GRCGYC 1 cut(s) 748
Hin1II CATG 8 cut(s) 199, 527, 568, 841, 914, 985, 1271, 1290
Hin6I GCGC 4 cut(s) 139, 481, 748, 1448
HinP1I GCGC 4 cut(s) 139, 481, 748, 1448
HincII GTYRAC 1 cut(s) 1227
HindII GTYRAC 1 cut(s) 1227
HindIII AAGCTT 2 cut(s) 1077, 1154
HinfI GANTC 5 cut(s) 804, 1052, 1057, 1178, 1242
HpaI GTTAAC 1 cut(s) 1227
HpaII CCGG 2 cut(s) 53, 375
HphI GGTGA 4 cut(s) 431, 527, 569, 683
Hpy166II GTNNAC 3 cut(s) 570, 671, 1227
Hpy188I TCNGA 4 cut(s) 433, 608, 1062, 1364
Hpy188III TCNNGA 6 cut(s) 10, 59, 237, 440, 880, 1235
Hpy8I GTNNAC 3 cut(s) 570, 671, 1227
Hpy99I CGWCG 2 cut(s) 180, 740
HpyAV CCTTC 5 cut(s) 202, 485, 793, 1001, 1298
HpyCH4III ACNGT 4 cut(s) 675, 705, 792, 814
HpyCH4IV ACGT 2 cut(s) 178, 972
HpyCH4V TGCA 6 cut(s) 253, 597, 829, 848, 914, 1271
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 6 cut(s) 20, 95, 533, 963, 1391, 1491
HpySE526I ACGT 2 cut(s) 178, 972
Hsp92I GRCGYC 1 cut(s) 748
Hsp92II CATG 8 cut(s) 199, 527, 568, 841, 914, 985, 1271, 1290
HspAI GCGC 4 cut(s) 139, 481, 748, 1448
KasI GGCGCC 1 cut(s) 747
Ksp22I TGATCA 1 cut(s) 436
KspAI GTTAAC 1 cut(s) 1227
KspI CCGCGG 1 cut(s) 697
Kzo9I GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
LmnI GCTCC 4 cut(s) 136, 146, 172, 1400
Lsp1109I GCAGC 6 cut(s) 261, 626, 1099, 1323, 1380, 1383
MaeI CTAG 4 cut(s) 611, 1256, 1322, 1344
MaeII ACGT 2 cut(s) 178, 972
MaeIII GTNAC 3 cut(s) 508, 515, 526
MalI GATC 6 cut(s) 438, 690, 878, 936, 1113, 1389
MboI GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
MboII GAAGA 4 cut(s) 176, 320, 378, 1075
MfeI CAATTG 1 cut(s) 1452
MflI RGATCY 3 cut(s) 876, 1111, 1387
MhlI GDGCHC 1 cut(s) 1122
MlsI TGGCCA 2 cut(s) 629, 1026
MluCI AATT 7 cut(s) 47, 333, 651, 988, 1217, 1276, 1452
MluNI TGGCCA 2 cut(s) 629, 1026
Mly113I GGCGCC 1 cut(s) 748
MlyI GAGTC 2 cut(s) 1046, 1236
MmeI TCCRAC 3 cut(s) 114, 150, 909
Mox20I TGGCCA 2 cut(s) 629, 1026
Mph1103I ATGCAT 2 cut(s) 850, 1273
MscI TGGCCA 2 cut(s) 629, 1026
MseI TTAA 2 cut(s) 684, 1226
MslI CAYNNNNRTG 2 cut(s) 21, 1236
Msp20I TGGCCA 2 cut(s) 629, 1026
MspA1I CMGCKG 1 cut(s) 696
MspI CCGG 2 cut(s) 53, 375
MspR9I CCNGG 1 cut(s) 752
MunI CAATTG 1 cut(s) 1452
MvaI CCWGG 1 cut(s) 752
MvnI CGCG 3 cut(s) 321, 483, 696
MwoI GCNNNNNNNGC 1 cut(s) 33
NarI GGCGCC 1 cut(s) 748
NdeII GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
NlaIII CATG 8 cut(s) 199, 527, 568, 841, 914, 985, 1271, 1290
NlaIV GGNNCC 5 cut(s) 57, 379, 749, 775, 1119
NmuCI GTSAC 2 cut(s) 508, 515
NsiI ATGCAT 2 cut(s) 850, 1273
NspI RCATGY 2 cut(s) 527, 914
NspV TTCGAA 1 cut(s) 649
PaeI GCATGC 1 cut(s) 914
PaeR7I CTCGAG 1 cut(s) 618
PciI ACATGT 1 cut(s) 523
PfeI GAWTC 3 cut(s) 804, 1057, 1178
PkrI GCNGC 9 cut(s) 248, 276, 616, 1089, 1209, 1313, 1370, 1373, 1428
PleI GAGTC 2 cut(s) 1046, 1236
PluTI GGCGCC 1 cut(s) 751
PpsI GAGTC 2 cut(s) 1046, 1236
PscI ACATGT 1 cut(s) 523
PshAI GACNNNNGTC 1 cut(s) 975
Psp6I CCWGG 1 cut(s) 750
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 5 cut(s) 57, 379, 749, 775, 1119
PspPI GGNCC 3 cut(s) 210, 377, 450
PspXI VCTCGAGB 1 cut(s) 618
PstI CTGCAG 1 cut(s) 831
PsuI RGATCY 3 cut(s) 876, 1111, 1387
RsaI GTAC 5 cut(s) 232, 355, 794, 836, 906
RsaNI GTAC 5 cut(s) 231, 354, 793, 835, 905
RseI CAYNNNNRTG 2 cut(s) 21, 1236
SacII CCGCGG 1 cut(s) 697
SaqAI TTAA 2 cut(s) 684, 1226
SatI GCNGC 9 cut(s) 247, 275, 615, 1088, 1208, 1312, 1369, 1372, 1427
Sau3AI GATC 6 cut(s) 436, 688, 876, 934, 1111, 1387
Sau96I GGNCC 3 cut(s) 210, 377, 450
ScaI AGTACT 1 cut(s) 355
SchI GAGTC 2 cut(s) 1046, 1236
ScrFI CCNGG 1 cut(s) 752
SduI GDGCHC 1 cut(s) 1122
SfcI CTRYAG 2 cut(s) 363, 827
SfoI GGCGCC 1 cut(s) 749
Sfr274I CTCGAG 1 cut(s) 618
Sfr303I CCGCGG 1 cut(s) 697
SfuI TTCGAA 1 cut(s) 649
SgrBI CCGCGG 1 cut(s) 697
SinI GGWCC 2 cut(s) 377, 450
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 2 cut(s) 21, 1236
SmlI CTYRAG 2 cut(s) 580, 618
SmoI CTYRAG 2 cut(s) 580, 618
SphI GCATGC 1 cut(s) 914
Sse9I AATT 7 cut(s) 47, 333, 651, 988, 1217, 1276, 1452
SsiI CCGC 6 cut(s) 246, 319, 694, 696, 1207, 1427
SspDI GGCGCC 1 cut(s) 747
SspMI CTAG 4 cut(s) 611, 1256, 1322, 1344
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 4 cut(s) 675, 705, 792, 814
TaiI ACGT 2 cut(s) 181, 975
TaqII GACCGA 1 cut(s) 467
TasI AATT 7 cut(s) 47, 333, 651, 988, 1217, 1276, 1452
TatI WGTACW 2 cut(s) 230, 353
TauI GCSGC 3 cut(s) 249, 1210, 1429
TfiI GAWTC 3 cut(s) 804, 1057, 1178
Tru1I TTAA 2 cut(s) 684, 1226
Tru9I TTAA 2 cut(s) 684, 1226
TscAI CASTG 1 cut(s) 537
TseFI GTSAC 2 cut(s) 508, 515
TseI GCWGC 6 cut(s) 274, 614, 1087, 1311, 1368, 1371
Tsp45I GTSAC 2 cut(s) 508, 515
TspDTI ATGAA 5 cut(s) 17, 184, 854, 1059, 1170
TspGWI ACGGA 2 cut(s) 125, 1425
TspRI CASTG 1 cut(s) 537
VpaK11BI GGWCC 2 cut(s) 377, 450
XapI RAATTY 3 cut(s) 47, 1217, 1276
XceI RCATGY 2 cut(s) 527, 914
XhoI CTCGAG 1 cut(s) 618
XspI CTAG 4 cut(s) 611, 1256, 1322, 1344
ZrmI AGTACT 1 cut(s) 355
Zsp2I ATGCAT 2 cut(s) 850, 1273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.