MD12G1208500.v1.1

Calmodulin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007733
Physical Location & Seq
Forward (+)
28791096 .. 28794761
3666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1208500.v1.1.491

Sequence Viewer

Length: 1755 bp
ATGGTCCCCAAAAGGCATTTTCGCGACGACGAGGGAAGCGAGGACTTCAAGTTTCCGGTTCCAGAATCCAAAAGGAGGCCTACTTTTAGGAATGTTGTTAGGGATGTGATGAGAGACGCCACGCTGAATGAACGCGAGTGGGAACGCTTCTTTCGGGGAATTGTGCGAGAGGAGGTGGAGCGCGGTATTCTTCCCTTCCTCATGTCATCTCCAAGCGTATCCAGGCCGCCGCTGGAAAGTGGGAGTACTTCTGGAGGATGTGGCTTGCAGTTGCGTTTTATCAACAAGCTGCTAAGTACCATATTCACAGGCAGTAAGGTGGGAGCAGAGAACGGTGCGCCTCTTCAAATTGAACTAGTCGATGCCAGTACTGGTACTATAGTCCGTTCCGGTCCCCTATCTTCGCTAAAGATTGAACTTCTTGTCGTGAATGGTGAGTTCGGGTCTGATGATCAAGAGGATTGGACCGAACGAGAATTCAACAACTACATTGTTCGTGCAAGGGATGGCAGGAGGCCATTGGTGACGGGTGAGATACAAGTTACACTAAGAGAGGGAGTTGGTCTTGTTGGTGATGTTGTGTTTACTGACAACTCGAGCTGGATAAGAAGCCGAACGTTCAGACTAGCAGCTCGAGTTGTGGCCAAATCTTCGAGTGAAGTACGAATTAGAGAAGCTAGAAGTGAACCGTTTGTGGTTAAAGATCACCGTGGAGAATTGTATAAGAAGCACTACCCTCCACACCTAACCGACGAAATATGGCGCCTGGAAAAGATAGCGAAAGACGGAGCCTTCCATAAGAGACTCTGCGAGAATGGAATCAGCACAGTGGAGGACTTCCTGCGGTTGTACATGAGAGATGCATCCTCGCTACGAAATGCTTTCGGTGGGATCTCAAACAAGATATGGGAAACAATCCTAGAGCATGCTATGACCTGTAAGTTGGATGACCATAAGTATTATGCCTTCCATAGAGCTGAGGAGGGTGTAAGTCTCATTTTCAATTCCATCCACAGGCTTTTAGGGGCAATAATCAATGGCCAGTTCTGTTCTCTGGATGAACTCGACGTGCATCAGAAGATGCTGGTGGAAACTCTGAAGCAGCATGCTTATCAAGATGTAAGAGATTTGGTCCCTGTCGATGCTTCGACTATATTTGGCCTTTCGAGGCCCTTGCCATGTCCACAACCTGAGCAAGTCACCAGTCCAAACCCTGATCTGCAACAAATTCAATTTCAGTTCACACATCAAGATGAACTCCCTATACAACTAGGTTTCAACCATGGATCAGCTTCAACCTCATCTGCCTACCAATCAGAAGGCAGCAGCAATCAGTTAATGGTTTCTCTAGCACAAAGCCAACCAATGCAATTCCGAAGCAACAGCTTTTCGGTGGAGGACTTTAACTCCGTCATCTACAATGGAGAAAGCAGTTGGCCGCCTCTCGGTGGTTTCCAAGCGCCAATTCTCCCAACCGGTCATTTAGGGACAGAAAACCTTTTCCAGGTCCAAGCATCAACTTGGTCTCCTGCAAACCCAATATGGGAACAAGCAGGCGGCTTCTACTTCACCTCAGGTAGCGAAGCAGAAGCCGCACATTTCCCTCTTCCTGCTTTTGTACATAACGCAGGGACTAGACAGAAACCCAAGGCATGCTGGTGCAAGCTTCGTGCCGCCATTAAATGGTGGGTTTCGGTTAGGAGGCATGGGATTGCTAGAAGAATGGCAAGGTCTCCTATGTATCTGAACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0002229 GO:0002237 GO:0002239 GO:0002376 GO:0002682 GO:0002831 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006725 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009696 GO:0009697 GO:0009787 GO:0009789 GO:0009814 GO:0009816 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010646 GO:0010647 GO:0010817 GO:0016053 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0031347 GO:0032101 GO:0032350 GO:0032787 GO:0033554 GO:0036293 GO:0036294 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0043900 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0046189 GO:0046394 GO:0046885 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070482 GO:0070887 GO:0071216 GO:0071219 GO:0071453 GO:0071456 GO:0071704 GO:0072330 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901421 GO:1901576 GO:1901615 GO:1901617 GO:1902584 GO:1903506 GO:1905957 GO:1905959 GO:2000070 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

585

Amino Acids

65.96

Weight (kDa)

7.01

Isoelectric Point (pI)

51.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 89 - 236 2.2e-59 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 249 - 313 2.9e-29 Calmodulin binding protein central domain
Calmod_bind_C PF20452 319 - 379 1.1e-17 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 762
AccB7I CCANNNNNTGG 1 cut(s) 1683
AccII CGCG 3 cut(s) 24, 135, 183
AciI CCGC 8 cut(s) 183, 227, 230, 844, 1439, 1557, 1593, 1674
AclI AACGTT 1 cut(s) 617
AclWI GGATC 2 cut(s) 899, 1294
AcoI YGGCCR 3 cut(s) 642, 1039, 1436
AcsI RAATTY 2 cut(s) 476, 1227
AcuI CTGAAG 1 cut(s) 1118
AcyI GRCGYC 2 cut(s) 117, 763
AfaI GTAC 7 cut(s) 247, 298, 370, 376, 663, 851, 1620
AfiI CCNNNNNNNGG 7 cut(s) 75, 1014, 1445, 1448, 1504, 1543, 1683
AgeI ACCGGT 1 cut(s) 1475
AgsI TTSAA 9 cut(s) 49, 347, 353, 416, 481, 1003, 1232, 1279, 1296
AhlI ACTAGT 1 cut(s) 355
AjiI CACGTC 1 cut(s) 1069
AjnI CCWGG 3 cut(s) 221, 765, 1503
AjuI GAANNNNNNNTTGG 2 cut(s) 1449, 1481
AluBI AGCT 8 cut(s) 289, 600, 632, 677, 977, 1292, 1386, 1666
AluI AGCT 8 cut(s) 289, 600, 632, 677, 977, 1292, 1386, 1666
Alw26I GTCTC 5 cut(s) 108, 796, 998, 1530, 1737
AlwI GGATC 2 cut(s) 899, 1294
Ama87I CYCGRG 2 cut(s) 595, 633
AoxI GGCC 8 cut(s) 77, 224, 515, 642, 1039, 1159, 1169, 1436
ApeKI GCWGC 5 cut(s) 289, 629, 1102, 1323, 1326
ApoI RAATTY 2 cut(s) 476, 1227
AsiGI ACCGGT 1 cut(s) 1475
AspLEI GCGC 4 cut(s) 183, 340, 765, 1462
AspS9I GGNCC 6 cut(s) 4, 392, 465, 1132, 1170, 1507
AsuHPI GGTGA 7 cut(s) 446, 535, 542, 584, 698, 1192, 1561
AvaI CYCGRG 2 cut(s) 595, 633
AvaII GGWCC 5 cut(s) 4, 392, 465, 1132, 1507
AxyI CCTNAGG 1 cut(s) 1573
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 2 cut(s) 644, 1041
BanI GGYRCC 1 cut(s) 762
BarI GAAGNNNNNNTAC 2 cut(s) 950, 982
BbvCI CCTCAGC 1 cut(s) 978
BbvI GCAGC 5 cut(s) 276, 641, 1114, 1335, 1338
BccI CCATC 2 cut(s) 500, 1016
BcgI CGANNNNNNTGC 2 cut(s) 1156, 1190
BciT130I CCWGG 3 cut(s) 223, 767, 1505
BciVI GTATCC 1 cut(s) 229
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 5 cut(s) 108, 796, 998, 1530, 1737
BcuI ACTAGT 1 cut(s) 355
BfaI CTAG 9 cut(s) 356, 626, 678, 920, 1271, 1349, 1635, 1716, 1753
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 2 cut(s) 766, 1463
BfuI GTATCC 1 cut(s) 229
BmcAI AGTACT 2 cut(s) 247, 370
Bme1390I CCNGG 3 cut(s) 223, 767, 1505
Bme18I GGWCC 5 cut(s) 4, 392, 465, 1132, 1507
BmeT110I CYCGRG 2 cut(s) 595, 633
BmgBI CACGTC 1 cut(s) 1069
BmgT120I GGNCC 6 cut(s) 4, 392, 465, 1132, 1170, 1507
BmiI GGNNCC 6 cut(s) 6, 60, 394, 764, 790, 1134
BmrFI CCNGG 3 cut(s) 223, 767, 1505
BmsI GCATC 7 cut(s) 352, 850, 872, 1071, 1081, 1132, 1523
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 2 cut(s) 978, 1191
BsaHI GRCGYC 2 cut(s) 117, 763
BsaI GGTCTC 2 cut(s) 1530, 1737
BsaJI CCNNGG 3 cut(s) 709, 1282, 1647
BsaWI WCCGGW 3 cut(s) 55, 389, 1475
BsaXI ACNNNNNCTCC 4 cut(s) 1391, 1421, 1510, 1540
Bsc4I CCNNNNNNNGG 7 cut(s) 75, 1014, 1445, 1448, 1504, 1543, 1683
Bse118I RCCGGY 1 cut(s) 1475
Bse1I ACTGG 4 cut(s) 366, 376, 1042, 1203
Bse21I CCTNAGG 1 cut(s) 1573
BseBI CCWGG 3 cut(s) 223, 767, 1505
BseDI CCNNGG 3 cut(s) 709, 1282, 1647
BseGI GGATG 7 cut(s) 109, 263, 511, 863, 952, 1008, 1063
BseLI CCNNNNNNNGG 7 cut(s) 75, 1014, 1445, 1448, 1504, 1543, 1683
BseMII CTCAG 3 cut(s) 969, 1182, 1587
BseNI ACTGG 4 cut(s) 366, 376, 1042, 1203
BseRI GAGGAG 2 cut(s) 185, 995
BseXI GCAGC 5 cut(s) 276, 641, 1114, 1335, 1338
Bsh1236I CGCG 3 cut(s) 24, 135, 183
BshFI GGCC 8 cut(s) 79, 226, 517, 644, 1041, 1161, 1171, 1438
BshNI GGYRCC 1 cut(s) 762
BshTI ACCGGT 1 cut(s) 1475
BsiHKCI CYCGRG 2 cut(s) 595, 633
BsiSI CCGG 3 cut(s) 56, 390, 1476
BslFI GGGAC 4 cut(s) 378, 1118, 1501, 1645
BslI CCNNNNNNNGG 7 cut(s) 75, 1014, 1445, 1448, 1504, 1543, 1683
BsmAI GTCTC 5 cut(s) 108, 796, 998, 1530, 1737
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 4 cut(s) 378, 1118, 1501, 1645
BsnI GGCC 8 cut(s) 79, 226, 517, 644, 1041, 1161, 1171, 1438
Bso31I GGTCTC 2 cut(s) 1530, 1737
BsoBI CYCGRG 2 cut(s) 595, 633
Bsp1407I TGTACA 2 cut(s) 849, 1618
Bsp143I GATC 5 cut(s) 451, 703, 891, 1216, 1286
Bsp19I CCATGG 1 cut(s) 1282
Bsp68I TCGCGA 1 cut(s) 24
BspACI CCGC 8 cut(s) 183, 227, 230, 844, 1439, 1557, 1593, 1674
BspANI GGCC 8 cut(s) 79, 226, 517, 644, 1041, 1161, 1171, 1438
BspCNI CTCAG 3 cut(s) 970, 1183, 1586
BspFNI CGCG 3 cut(s) 24, 135, 183
BspLI GGNNCC 6 cut(s) 6, 60, 394, 764, 790, 1134
BspPI GGATC 2 cut(s) 899, 1294
BspT107I GGYRCC 1 cut(s) 762
BspTNI GGTCTC 2 cut(s) 1530, 1737
BsrFI RCCGGY 1 cut(s) 1475
BsrGI TGTACA 2 cut(s) 849, 1618
BsrI ACTGG 4 cut(s) 366, 376, 1042, 1203
BssAI RCCGGY 1 cut(s) 1475
BssECI CCNNGG 3 cut(s) 709, 1282, 1647
BssMI GATC 5 cut(s) 451, 703, 891, 1216, 1286
BssNI GRCGYC 2 cut(s) 117, 763
BssT1I CCWWGG 2 cut(s) 1282, 1647
Bst2UI CCWGG 3 cut(s) 223, 767, 1505
Bst4CI ACNGT 4 cut(s) 335, 690, 710, 829
Bst6I CTCTTC 2 cut(s) 348, 1611
BstACI GRCGYC 2 cut(s) 117, 763
BstAUI TGTACA 2 cut(s) 849, 1618
BstC8I GCNNGC 6 cut(s) 266, 927, 1107, 1555, 1654, 1664
BstDEI CTNAG 5 cut(s) 293, 548, 978, 1191, 1573
BstDSI CCRYGG 2 cut(s) 709, 1282
BstENI CCTNNNNNAGG 1 cut(s) 1502
BstF5I GGATG 7 cut(s) 109, 263, 511, 863, 952, 1008, 1063
BstFNI CGCG 3 cut(s) 24, 135, 183
BstH2I RGCGCY 2 cut(s) 766, 1463
BstHHI GCGC 4 cut(s) 183, 340, 765, 1462
BstKTI GATC 5 cut(s) 454, 706, 894, 1219, 1289
BstMAI GTCTC 5 cut(s) 108, 796, 998, 1530, 1737
BstMBI GATC 5 cut(s) 451, 703, 891, 1216, 1286
BstMWI GCNNNNNNNGC 1 cut(s) 1592
BstNI CCWGG 3 cut(s) 223, 767, 1505
BstNSI RCATGY 3 cut(s) 929, 1109, 1656
BstSCI CCNGG 3 cut(s) 221, 765, 1503
BstSFI CTRYAG 1 cut(s) 378
BstUI CGCG 3 cut(s) 24, 135, 183
BstV1I GCAGC 5 cut(s) 276, 641, 1114, 1335, 1338
BstX2I RGATCY 1 cut(s) 891
BstYI RGATCY 1 cut(s) 891
Bsu36I CCTNAGG 1 cut(s) 1573
BsuI GTATCC 1 cut(s) 229
BsuRI GGCC 8 cut(s) 79, 226, 517, 644, 1041, 1161, 1171, 1438
BtgI CCRYGG 2 cut(s) 709, 1282
BtrI CACGTC 1 cut(s) 1069
BtsCI GGATG 7 cut(s) 109, 263, 511, 863, 952, 1008, 1063
BtsIMutI CAGTG 1 cut(s) 834
BtuMI TCGCGA 1 cut(s) 24
Cac8I GCNNGC 6 cut(s) 266, 927, 1107, 1555, 1654, 1664
CfoI GCGC 4 cut(s) 183, 340, 765, 1462
Cfr10I RCCGGY 1 cut(s) 1475
Cfr13I GGNCC 6 cut(s) 4, 392, 465, 1132, 1170, 1507
CseI GACGC 1 cut(s) 125
Csp6I GTAC 7 cut(s) 246, 297, 369, 375, 662, 850, 1619
CspAI ACCGGT 1 cut(s) 1475
CviAII CATG 8 cut(s) 202, 853, 926, 1106, 1179, 1283, 1653, 1706
CviQI GTAC 7 cut(s) 246, 297, 369, 375, 662, 850, 1619
DdeI CTNAG 5 cut(s) 293, 548, 978, 1191, 1573
DinI GGCGCC 1 cut(s) 764
DpnI GATC 5 cut(s) 453, 705, 893, 1218, 1288
DpnII GATC 5 cut(s) 451, 703, 891, 1216, 1286
EaeI YGGCCR 3 cut(s) 642, 1039, 1436
Eam1104I CTCTTC 2 cut(s) 348, 1611
EarI CTCTTC 2 cut(s) 348, 1611
Eco130I CCWWGG 2 cut(s) 1282, 1647
Eco147I AGGCCT 1 cut(s) 79
Eco31I GGTCTC 2 cut(s) 1530, 1737
Eco47I GGWCC 5 cut(s) 4, 392, 465, 1132, 1507
Eco57I CTGAAG 1 cut(s) 1118
Eco81I CCTNAGG 1 cut(s) 1573
Eco88I CYCGRG 2 cut(s) 595, 633
EcoNI CCTNNNNNAGG 1 cut(s) 1502
EcoO109I RGGNCCY 1 cut(s) 1170
EcoRI GAATTC 1 cut(s) 476
EcoRII CCWGG 3 cut(s) 221, 765, 1503
EcoT14I CCWWGG 2 cut(s) 1282, 1647
EcoT22I ATGCAT 1 cut(s) 865
EgeI GGCGCC 1 cut(s) 764
EheI GGCGCC 1 cut(s) 764
ErhI CCWWGG 2 cut(s) 1282, 1647
Esp3I CGTCTC 1 cut(s) 108
FaeI CATG 8 cut(s) 205, 856, 929, 1109, 1182, 1286, 1656, 1709
FaqI GGGAC 4 cut(s) 378, 1118, 1501, 1645
FatI CATG 8 cut(s) 201, 852, 925, 1105, 1178, 1282, 1652, 1705
FbaI TGATCA 1 cut(s) 451
FokI GGATG 7 cut(s) 116, 270, 518, 850, 959, 995, 1070
FspBI CTAG 9 cut(s) 356, 626, 678, 920, 1271, 1349, 1635, 1716, 1753
GlaI GCGC 4 cut(s) 182, 339, 764, 1461
GsuI CTGGAG 1 cut(s) 273
HaeII RGCGCY 2 cut(s) 766, 1463
HaeIII GGCC 8 cut(s) 79, 226, 517, 644, 1041, 1161, 1171, 1438
HapII CCGG 3 cut(s) 56, 390, 1476
HgaI GACGC 1 cut(s) 125
HhaI GCGC 4 cut(s) 183, 340, 765, 1462
Hin1I GRCGYC 2 cut(s) 117, 763
Hin1II CATG 8 cut(s) 205, 856, 929, 1109, 1182, 1286, 1656, 1709
Hin6I GCGC 4 cut(s) 181, 338, 763, 1460
HinP1I GCGC 4 cut(s) 181, 338, 763, 1460
HindIII AAGCTT 1 cut(s) 1664
HinfI GANTC 3 cut(s) 65, 804, 819
HpaII CCGG 3 cut(s) 56, 390, 1476
HphI GGTGA 7 cut(s) 446, 535, 542, 584, 698, 1192, 1561
Hpy166II GTNNAC 4 cut(s) 585, 686, 1184, 1242
Hpy188I TCNGA 7 cut(s) 448, 623, 1077, 1098, 1318, 1376, 1746
Hpy188III TCNNGA 8 cut(s) 23, 62, 252, 427, 455, 1055, 1115, 1250
Hpy8I GTNNAC 4 cut(s) 585, 686, 1184, 1242
Hpy99I CGWCG 4 cut(s) 29, 32, 755, 1070
HpyAV CCTTC 4 cut(s) 205, 802, 976, 1313
HpyCH4III ACNGT 4 cut(s) 335, 690, 710, 829
HpyCH4IV ACGT 2 cut(s) 617, 1068
HpyCH4V TGCA 8 cut(s) 268, 500, 863, 1072, 1222, 1369, 1532, 1662
HpyF10VI GCNNNNNNNGC 1 cut(s) 1592
HpyF3I CTNAG 5 cut(s) 293, 548, 978, 1191, 1573
HpySE526I ACGT 2 cut(s) 617, 1068
Hsp92I GRCGYC 2 cut(s) 117, 763
Hsp92II CATG 8 cut(s) 205, 856, 929, 1109, 1182, 1286, 1656, 1709
HspAI GCGC 4 cut(s) 181, 338, 763, 1460
KasI GGCGCC 1 cut(s) 762
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 5 cut(s) 451, 703, 891, 1216, 1286
LmnI GCTCC 3 cut(s) 178, 323, 788
Lsp1109I GCAGC 5 cut(s) 276, 641, 1114, 1335, 1338
LweI GCATC 7 cut(s) 352, 850, 872, 1071, 1081, 1132, 1523
MaeI CTAG 9 cut(s) 356, 626, 678, 920, 1271, 1349, 1635, 1716, 1753
MaeII ACGT 2 cut(s) 617, 1068
MaeIII GTNAC 3 cut(s) 523, 541, 1198
MalI GATC 5 cut(s) 453, 705, 893, 1218, 1288
MboI GATC 5 cut(s) 451, 703, 891, 1216, 1286
MboII GAAGA 7 cut(s) 182, 335, 393, 642, 1090, 1598, 1731
MflI RGATCY 1 cut(s) 891
MlsI TGGCCA 2 cut(s) 644, 1041
MluNI TGGCCA 2 cut(s) 644, 1041
Mly113I GGCGCC 1 cut(s) 763
MlyI GAGTC 1 cut(s) 798
MmeI TCCRAC 1 cut(s) 924
Mox20I TGGCCA 2 cut(s) 644, 1041
Mph1103I ATGCAT 1 cut(s) 865
MscI TGGCCA 2 cut(s) 644, 1041
MseI TTAA 4 cut(s) 699, 1337, 1404, 1680
MslI CAYNNNNRTG 2 cut(s) 1251, 1657
Msp20I TGGCCA 2 cut(s) 644, 1041
MspA1I CMGCKG 1 cut(s) 232
MspI CCGG 3 cut(s) 56, 390, 1476
MspR9I CCNGG 3 cut(s) 223, 767, 1505
MvaI CCWGG 3 cut(s) 223, 767, 1505
MvnI CGCG 3 cut(s) 24, 135, 183
MwoI GCNNNNNNNGC 1 cut(s) 1592
NarI GGCGCC 1 cut(s) 763
NcoI CCATGG 1 cut(s) 1282
NdeII GATC 5 cut(s) 451, 703, 891, 1216, 1286
NlaIII CATG 8 cut(s) 205, 856, 929, 1109, 1182, 1286, 1656, 1709
NlaIV GGNNCC 6 cut(s) 6, 60, 394, 764, 790, 1134
NmuCI GTSAC 2 cut(s) 523, 1198
NruI TCGCGA 1 cut(s) 24
NsiI ATGCAT 1 cut(s) 865
NspI RCATGY 3 cut(s) 929, 1109, 1656
PaeI GCATGC 3 cut(s) 929, 1109, 1656
PaeR7I CTCGAG 2 cut(s) 595, 633
PceI AGGCCT 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PfeI GAWTC 2 cut(s) 65, 819
PflMI CCANNNNNTGG 1 cut(s) 1683
PinAI ACCGGT 1 cut(s) 1475
PleI GAGTC 1 cut(s) 798
PluTI GGCGCC 1 cut(s) 766
PpsI GAGTC 1 cut(s) 798
Psp1406I AACGTT 1 cut(s) 617
Psp6I CCWGG 3 cut(s) 221, 765, 1503
PspGI CCWGG 3 cut(s) 221, 765, 1503
PspN4I GGNNCC 6 cut(s) 6, 60, 394, 764, 790, 1134
PspPI GGNCC 6 cut(s) 4, 392, 465, 1132, 1170, 1507
PspXI VCTCGAGB 2 cut(s) 595, 633
PsrI GAACNNNNNNTAC 2 cut(s) 1248, 1280
PsuI RGATCY 1 cut(s) 891
RruI TCGCGA 1 cut(s) 24
RsaI GTAC 7 cut(s) 247, 298, 370, 376, 663, 851, 1620
RsaNI GTAC 7 cut(s) 246, 297, 369, 375, 662, 850, 1619
RseI CAYNNNNRTG 2 cut(s) 1251, 1657
SaqAI TTAA 4 cut(s) 699, 1337, 1404, 1680
Sau3AI GATC 5 cut(s) 451, 703, 891, 1216, 1286
Sau96I GGNCC 6 cut(s) 4, 392, 465, 1132, 1170, 1507
ScaI AGTACT 2 cut(s) 247, 370
SchI GAGTC 1 cut(s) 798
ScrFI CCNGG 3 cut(s) 223, 767, 1505
SfaNI GCATC 7 cut(s) 352, 850, 872, 1071, 1081, 1132, 1523
SfcI CTRYAG 1 cut(s) 378
SfoI GGCGCC 1 cut(s) 764
Sfr274I CTCGAG 2 cut(s) 595, 633
SinI GGWCC 5 cut(s) 4, 392, 465, 1132, 1507
SlaI CTCGAG 2 cut(s) 595, 633
SmiMI CAYNNNNRTG 2 cut(s) 1251, 1657
SmlI CTYRAG 2 cut(s) 595, 633
SmoI CTYRAG 2 cut(s) 595, 633
SpeI ACTAGT 1 cut(s) 355
SphI GCATGC 3 cut(s) 929, 1109, 1656
SseBI AGGCCT 1 cut(s) 79
SsiI CCGC 8 cut(s) 183, 227, 230, 844, 1439, 1557, 1593, 1674
SspDI GGCGCC 1 cut(s) 762
SspMI CTAG 9 cut(s) 356, 626, 678, 920, 1271, 1349, 1635, 1716, 1753
StuI AGGCCT 1 cut(s) 79
StyD4I CCNGG 3 cut(s) 221, 765, 1503
StyI CCWWGG 2 cut(s) 1282, 1647
TaaI ACNGT 4 cut(s) 335, 690, 710, 829
TaiI ACGT 2 cut(s) 620, 1071
TaqI TCGA 8 cut(s) 360, 596, 634, 653, 1065, 1140, 1148, 1166
TaqII GACCGA 1 cut(s) 482
TatI WGTACW 4 cut(s) 245, 368, 849, 1618
TauI GCSGC 6 cut(s) 229, 232, 1441, 1560, 1595, 1676
TfiI GAWTC 2 cut(s) 65, 819
Tru1I TTAA 4 cut(s) 699, 1337, 1404, 1680
Tru9I TTAA 4 cut(s) 699, 1337, 1404, 1680
TscAI CASTG 1 cut(s) 834
TseFI GTSAC 2 cut(s) 523, 1198
TseI GCWGC 5 cut(s) 289, 629, 1102, 1323, 1326
Tsp45I GTSAC 2 cut(s) 523, 1198
TspDTI ATGAA 3 cut(s) 144, 1074, 1269
TspGWI ACGGA 3 cut(s) 374, 801, 1399
TspRI CASTG 1 cut(s) 834
Van91I CCANNNNNTGG 1 cut(s) 1683
VpaK11BI GGWCC 5 cut(s) 4, 392, 465, 1132, 1507
XagI CCTNNNNNAGG 1 cut(s) 1502
XapI RAATTY 2 cut(s) 476, 1227
XceI RCATGY 3 cut(s) 929, 1109, 1656
XcmI CCANNNNNNNNNTGG 1 cut(s) 229
XhoI CTCGAG 2 cut(s) 595, 633
XspI CTAG 9 cut(s) 356, 626, 678, 920, 1271, 1349, 1635, 1716, 1753
ZrmI AGTACT 2 cut(s) 247, 370
Zsp2I ATGCAT 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.