MD12G1208300.v1.1

Calmodulin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007733
Physical Location & Seq
Forward (+)
28783096 .. 28788053
4958 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1208300.v1.1.491

Sequence Viewer

Length: 2796 bp
ATGGTGCTCAAGAGGCATTTTCGTGACGACGAGGGAAGCGAGGACGTCGAGTTTCCGGTTCCAGAATCCAAAAGGAGGCCTAATTTTAGGGATGTTGTTAGGGATGTGATGAGAGACGCCACGCTGAATGAACGCGAGTCGGAACGCTTTCGGAGAATTGTGCGAGAGGAGGTGGAGCGCGGTATTCTTCCCTTCCTCATGTCATCTCCAAGTGTATCCAGGCCGCCGCTGGAAAGTGGGAGTGCTTCTGGAGGATGTGGCTTGCAGTTGCGTTTTATCAACAAGCTGCCAAGTACCATATTCACAGGCAGTAAGGTGGAAGCAGAGAATGGCACGCCTCTTCAAATTGAACTAGTCGATGCCAGTACCGGTGCTATAGTCCGTTCCGGTCCCCTATCTTCGCTAAAGATTGAACTTCTTGTCGTGAATGGTGAGTTCGGGTCTGATGATCAAGAGGATTGGACCGAACAAGAATTCAACAACTACATTGTTCGCGCAAGGGATGGCAGGAGGCCATTAGTGACGGGCGAGACACATGTTACACTAAGAGAGGGAGTTGGTTTTGTTGGTGATGTTGTGTTTACTGACAACTCAAGCTGGACAAGAAGCCGAAAGTTCAGACTAGCAGCTCGAGTTGTGGCCAAATCTCCGAGTGAAGTACGAATTAGAGAAGCTAGAAGTGAACCGTTTGTGGTTAAAGATCACCGTGGAGAATTGTATAAGAAGCACTACCCTCCACACCTAAACGATGAAATATGGCGCCTGGAAATGATAGCAAAAGACGGAGCCTTCCATAAGAGTCTGTGCGAGAATGGAATCAGCACAGTGGAGGACTTCCTGCAGTTGTACATGAGAGATGCATCCTTGCTACGAAATGCTTTCGGTGTGATCTCGAACAAGATATGGGAAACAATCATAAAGCATGCTATGGCCTGTAAGTTGGATGACCATAAGCGCTATGCCTATCATCGAGCTGAGCACGATGTAAGTCTCATGTTCAATTCCGTTCACAGGCTTGTAGGGGCAATAATCAATGGCCAGTTCTGTTCTCTGGATGAACTCGACGCGCATCAGAAGATGCTGGTGGAAACTCTGAAACAGCATGCTTATCGAAATGTAAGAGATTTGGTCCCCATCGATGTTTCGACTATATTTGGCCTTTCGAGGCCCTTGCCATGTCCCCAAGCTGAACAATTCAAAAATCAATTAGCAATAAGAGGGAGAGACCCCACGCTGAATGAACGCGAGTCGGAACGCTTCTTTCGGAGAATTATGCGAGAGGAGGTGGAGCGCGGTATTCTTCCCTTCCTCATGTCATCTCCAAGCGTATCCAGGCCGCCGCTGGAAAGTGGGAGTGCTTCTGGAGGATGTGGCTTGCAGTTGCGTTTTATCAACAAGCTGCCAAGTACCATATTCACAGGCAGTAAGGTGGAAGCAGAGAATGGCACGCCTCTTCAAATTGAACTTGTCGATGCCAGTACTGGTGCTATAGTCCGTTCCGGTCCCCTATCTTCGCTAAAGATTGAACTTCTTGTCGTGAATGGTGAGTTCGGGTCTGATGATCAAGATGATTGGACCGAACGAGAATTCAGCACCTACATTGTTCGCGCAAGGGATGGCAACATGCCATCAGTGACGGGTGAGATACATGTTACACTAAGAGAGGGAGTTGGTTTTGTCGGTGATGTTGTGTTTACTGACAACTCGGGCTGGATAAGAAGCCAAACGTTCAGACTAGCAGCTCGAGTTGTGGCCAAATCTTCGAGTGAAGTACGAATTAGAGAAGCTAGAAGTGAACCGTTTGTGGTTGGAGAATTTTATAAGAAGCACTATCTACACCTAAACGATGAAATATGGTGCCTGCAAAAGATAGCAAAAGACGGAGCCTTCCATAAGAGACTCTGCGAGAATGGAATCAGCACAGTCGAGGACTTCCTGCAGTTGTACATGAGAGATGCATCCTTGCTACGAAATGCTTTCGGTGTGATCTCGAACAAGTTATGGGAAACAATCATAGAGCATGCTATGACCTGTAAGTTGGATGACCATAGGTTCTATGCCTTCCATGGAGCTGAGGGGGATGTAAGTCTCATTTTCAATTCCATCCACAGGCTTGTAGCGGCAATAATCAATGGCCAGTTCTGTTTTCTGCATGAACTCCACGTGCATCAGATGATGCTGGTGGAAACTGTGAAGCAGCATGCTTATCAAAATGTAAAAGATTTGATCCCTGTCGATGCTTCGACTATATTTGGCCTTTCGAGGCCCTTGCCATGTCCACAAACTGAGCAATTCACCAGTCCAAACCCTGATCTGCAACAAATTCAATTTCAGTTCACACATCAAGATGAACTCCCAATACAACTAGGTTTCAACCACGGATCAGCTTCAACCTCATCTGCCTACCAAGCAGCAGGAAGCAGCAATCAGTTAATGGTTTCTCTAGCACAAAGCCAACCAATGCAACTCCGAAGCAACAGCTTTTCGGTGGGGGACTCTAACTCCGTCATCTACAGTGGAGAAAGCAGTTCGCCGCCTTTCGGTGGTTTCCAAGCGCCAATTCTCCCAACCGGTCATTTAGGGACAGAAAACCTTTTCCAGGTCGAAGCATCAACTTTGTCTCCTGCAAACCCAATATGGGGACAAGCAGGCGACTTCTACTTCACTTCAGGTAGCGAAGCAGAAGCTAGACGGAAAGCCAAGGCATGCTGGTGCAAGCTTCGTGCCGCCGTTAAGTGGATGTCGGTTAGGAGGCATATGACTGCAAGGCCTTCTACGTATCTGAACTTTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0002229 GO:0002237 GO:0002239 GO:0002376 GO:0002682 GO:0002831 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006725 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009696 GO:0009697 GO:0009787 GO:0009789 GO:0009814 GO:0009816 GO:0009889 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010112 GO:0010224 GO:0010337 GO:0010468 GO:0010556 GO:0010565 GO:0010646 GO:0010647 GO:0010817 GO:0016053 GO:0016999 GO:0017000 GO:0017144 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0023051 GO:0023056 GO:0031323 GO:0031326 GO:0031347 GO:0032101 GO:0032350 GO:0032787 GO:0033554 GO:0036293 GO:0036294 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0043900 GO:0044237 GO:0044249 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0046189 GO:0046394 GO:0046885 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0062012 GO:0065007 GO:0065008 GO:0070482 GO:0070887 GO:0071216 GO:0071219 GO:0071453 GO:0071456 GO:0071704 GO:0072330 GO:0080090 GO:0080134 GO:0080142 GO:0097159 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901419 GO:1901421 GO:1901576 GO:1901615 GO:1901617 GO:1902584 GO:1903506 GO:1905957 GO:1905959 GO:2000070 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

932

Amino Acids

104.75

Weight (kDa)

6.45

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 88 - 235 3.8e-60 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 248 - 312 7e-26 Calmodulin binding protein central domain
Calmod_bind_C PF20452 318 - 378 1.7e-18 Calmodulin binding protein C-terminal domain
Calmodulin_bind PF07887 459 - 603 7.8e-54 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 614 - 678 1.4e-25 Calmodulin binding protein central domain
Calmod_bind_C PF20452 686 - 744 1.2e-13 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1821, 2794
AatII GACGTC 1 cut(s) 48
AccB1I GGYRCC 2 cut(s) 759, 1857
AccII CGCG 7 cut(s) 135, 180, 495, 1067, 1245, 1293, 1608
AciI CCGC 9 cut(s) 180, 224, 227, 1293, 1337, 1340, 2120, 2534, 2727
AclI AACGTT 1 cut(s) 1727
AclWI GGATC 2 cut(s) 2221, 2389
AcoI YGGCCR 4 cut(s) 639, 1036, 1752, 2134
AcsI RAATTY 4 cut(s) 473, 1586, 1814, 2322
AcuI CTGAAG 1 cut(s) 2652
AcvI CACGTG 1 cut(s) 2164
AcyI GRCGYC 3 cut(s) 45, 117, 760
AfaI GTAC 8 cut(s) 295, 367, 660, 848, 1408, 1480, 1773, 1946
AfeI AGCGCT 1 cut(s) 956
AfiI CCNNNNNNNGG 9 cut(s) 75, 1011, 2109, 2540, 2543, 2599, 2638, 2639, 2736
AflIII ACRYGT 2 cut(s) 535, 1648
AgeI ACCGGT 2 cut(s) 368, 2570
AhlI ACTAGT 1 cut(s) 352
AjnI CCWGG 4 cut(s) 218, 762, 1331, 2598
AjuI GAANNNNNNNTTGG 2 cut(s) 2544, 2576
Alw21I GWGCWC 2 cut(s) 9, 981
Alw26I GTCTC 7 cut(s) 108, 524, 995, 1218, 1891, 2093, 2625
AlwI GGATC 2 cut(s) 2221, 2389
Ama87I CYCGRG 3 cut(s) 630, 1705, 1743
Aor51HI AGCGCT 1 cut(s) 956
ApeKI GCWGC 7 cut(s) 286, 626, 1399, 1739, 2197, 2411, 2421
ApoI RAATTY 4 cut(s) 473, 1586, 1814, 2322
AsiGI ACCGGT 2 cut(s) 368, 2570
Asp700I GAANNNNTTC 1 cut(s) 147
AspLEI GCGC 8 cut(s) 180, 497, 762, 957, 1069, 1293, 1610, 2557
AspS9I GGNCC 7 cut(s) 389, 462, 1129, 1167, 1502, 1575, 2265
AsuHPI GGTGA 7 cut(s) 443, 581, 695, 1556, 1652, 1694, 2287
AvaI CYCGRG 3 cut(s) 630, 1705, 1743
AvaII GGWCC 5 cut(s) 389, 462, 1129, 1502, 1575
BalI TGGCCA 4 cut(s) 641, 1038, 1754, 2136
BanI GGYRCC 2 cut(s) 759, 1857
BbrPI CACGTG 1 cut(s) 2164
Bbv12I GWGCWC 2 cut(s) 9, 981
BbvCI CCTCAGC 1 cut(s) 2073
BbvI GCAGC 7 cut(s) 273, 638, 1386, 1751, 2209, 2423, 2433
BccI CCATC 5 cut(s) 497, 1142, 1610, 1636, 2111
BceAI ACGGC 1 cut(s) 2714
BcgI CGANNNNNNTGC 6 cut(s) 1091, 1125, 1153, 1187, 2251, 2285
BciT130I CCWGG 4 cut(s) 220, 764, 1333, 2600
BciVI GTATCC 2 cut(s) 226, 1339
BclI TGATCA 2 cut(s) 448, 1561
BcoDI GTCTC 7 cut(s) 108, 524, 995, 1218, 1891, 2093, 2625
BcuI ACTAGT 1 cut(s) 352
BfaI CTAG 8 cut(s) 353, 623, 675, 1736, 1788, 2366, 2444, 2688
BfmI CTRYAG 5 cut(s) 375, 839, 1488, 1937, 2512
BfoI RGCGCY 3 cut(s) 763, 958, 2558
BfuI GTATCC 2 cut(s) 226, 1339
BlpI GCTNAGC 1 cut(s) 975
BmcAI AGTACT 1 cut(s) 1480
Bme1390I CCNGG 4 cut(s) 220, 764, 1333, 2600
Bme18I GGWCC 5 cut(s) 389, 462, 1129, 1502, 1575
BmeT110I CYCGRG 3 cut(s) 630, 1705, 1743
BmgT120I GGNCC 7 cut(s) 389, 462, 1129, 1167, 1502, 1575, 2265
BmiI GGNNCC 8 cut(s) 60, 391, 761, 787, 1131, 1504, 1859, 1885
BmrFI CCNGG 4 cut(s) 220, 764, 1333, 2600
BpmI CTGGAG 2 cut(s) 270, 1383
Bpu10I CCTNAGC 1 cut(s) 2073
Bpu1102I GCTNAGC 1 cut(s) 975
BpuEI CTTGAG 1 cut(s) 577
Bsa29I ATCGAT 1 cut(s) 1137
BsaAI YACGTR 2 cut(s) 2164, 2778
BsaHI GRCGYC 3 cut(s) 45, 117, 760
BsaI GGTCTC 1 cut(s) 1218
BsaJI CCNNGG 4 cut(s) 706, 2065, 2377, 2700
BsaWI WCCGGW 5 cut(s) 55, 368, 386, 1499, 2570
BsaXI ACNNNNNCTCC 4 cut(s) 2486, 2516, 2605, 2635
Bsc4I CCNNNNNNNGG 9 cut(s) 75, 1011, 2109, 2540, 2543, 2599, 2638, 2639, 2736
Bse118I RCCGGY 2 cut(s) 368, 2570
Bse1I ACTGG 6 cut(s) 363, 1039, 1476, 1486, 2137, 2298
BseBI CCWGG 4 cut(s) 220, 764, 1333, 2600
BseCI ATCGAT 1 cut(s) 1137
BseDI CCNNGG 4 cut(s) 706, 2065, 2377, 2700
BseLI CCNNNNNNNGG 9 cut(s) 75, 1011, 2109, 2540, 2543, 2599, 2638, 2639, 2736
BseMII CTCAG 3 cut(s) 966, 2064, 2277
BseNI ACTGG 6 cut(s) 363, 1039, 1476, 1486, 2137, 2298
BseRI GAGGAG 2 cut(s) 182, 1295
BseXI GCAGC 7 cut(s) 273, 638, 1386, 1751, 2209, 2423, 2433
Bsh1236I CGCG 7 cut(s) 135, 180, 495, 1067, 1245, 1293, 1608
BshNI GGYRCC 2 cut(s) 759, 1857
BshTI ACCGGT 2 cut(s) 368, 2570
BshVI ATCGAT 1 cut(s) 1137
BsiHKAI GWGCWC 2 cut(s) 9, 981
BsiHKCI CYCGRG 3 cut(s) 630, 1705, 1743
BsiSI CCGG 5 cut(s) 56, 369, 387, 1500, 2571
BslFI GGGAC 7 cut(s) 375, 1115, 1164, 1488, 2507, 2596, 2655
BslI CCNNNNNNNGG 9 cut(s) 75, 1011, 2109, 2540, 2543, 2599, 2638, 2639, 2736
BsmAI GTCTC 7 cut(s) 108, 524, 995, 1218, 1891, 2093, 2625
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 7 cut(s) 375, 1115, 1164, 1488, 2507, 2596, 2655
Bso31I GGTCTC 1 cut(s) 1218
BsoBI CYCGRG 3 cut(s) 630, 1705, 1743
Bsp1286I GDGCHC 2 cut(s) 9, 981
Bsp1407I TGTACA 2 cut(s) 846, 1944
Bsp143I GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
Bsp1720I GCTNAGC 1 cut(s) 975
Bsp19I CCATGG 1 cut(s) 2065
BspACI CCGC 9 cut(s) 180, 224, 227, 1293, 1337, 1340, 2120, 2534, 2727
BspCNI CTCAG 3 cut(s) 967, 2065, 2278
BspDI ATCGAT 1 cut(s) 1137
BspFNI CGCG 7 cut(s) 135, 180, 495, 1067, 1245, 1293, 1608
BspLI GGNNCC 8 cut(s) 60, 391, 761, 787, 1131, 1504, 1859, 1885
BspMAI CTGCAG 2 cut(s) 843, 1941
BspPI GGATC 2 cut(s) 2221, 2389
BspT107I GGYRCC 2 cut(s) 759, 1857
BspTNI GGTCTC 1 cut(s) 1218
BsrFI RCCGGY 2 cut(s) 368, 2570
BsrGI TGTACA 2 cut(s) 846, 1944
BsrI ACTGG 6 cut(s) 363, 1039, 1476, 1486, 2137, 2298
BssAI RCCGGY 2 cut(s) 368, 2570
BssECI CCNNGG 4 cut(s) 706, 2065, 2377, 2700
BssMI GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
BssNI GRCGYC 3 cut(s) 45, 117, 760
BssT1I CCWWGG 2 cut(s) 2065, 2700
Bst2UI CCWGG 4 cut(s) 220, 764, 1333, 2600
Bst4CI ACNGT 7 cut(s) 687, 707, 826, 1800, 1924, 2191, 2516
Bst6I CTCTTC 2 cut(s) 345, 1458
BstACI GRCGYC 3 cut(s) 45, 117, 760
BstAUI TGTACA 2 cut(s) 846, 1944
BstBAI YACGTR 2 cut(s) 2164, 2778
BstDEI CTNAG 5 cut(s) 545, 975, 1658, 2073, 2286
BstDSI CCRYGG 3 cut(s) 706, 2065, 2377
BstENI CCTNNNNNAGG 1 cut(s) 2597
BstFNI CGCG 7 cut(s) 135, 180, 495, 1067, 1245, 1293, 1608
BstH2I RGCGCY 3 cut(s) 763, 958, 2558
BstHHI GCGC 8 cut(s) 180, 497, 762, 957, 1069, 1293, 1610, 2557
BstKTI GATC 8 cut(s) 451, 703, 891, 1564, 1989, 2229, 2314, 2384
BstMAI GTCTC 7 cut(s) 108, 524, 995, 1218, 1891, 2093, 2625
BstMBI GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
BstMWI GCNNNNNNNGC 2 cut(s) 13, 2408
BstNI CCWGG 4 cut(s) 220, 764, 1333, 2600
BstNSI RCATGY 8 cut(s) 539, 926, 1106, 1627, 1652, 2024, 2204, 2709
BstSCI CCNGG 4 cut(s) 218, 762, 1331, 2598
BstSFI CTRYAG 5 cut(s) 375, 839, 1488, 1937, 2512
BstSNI TACGTA 1 cut(s) 2778
BstUI CGCG 7 cut(s) 135, 180, 495, 1067, 1245, 1293, 1608
BstV1I GCAGC 7 cut(s) 273, 638, 1386, 1751, 2209, 2423, 2433
Bsu15I ATCGAT 1 cut(s) 1137
BsuI GTATCC 2 cut(s) 226, 1339
BsuTUI ATCGAT 1 cut(s) 1137
BtgI CCRYGG 3 cut(s) 706, 2065, 2377
BtsIMutI CAGTG 3 cut(s) 831, 1638, 2521
CfoI GCGC 8 cut(s) 180, 497, 762, 957, 1069, 1293, 1610, 2557
Cfr10I RCCGGY 2 cut(s) 368, 2570
Cfr13I GGNCC 7 cut(s) 389, 462, 1129, 1167, 1502, 1575, 2265
ClaI ATCGAT 1 cut(s) 1137
CseI GACGC 2 cut(s) 125, 1073
Csp6I GTAC 8 cut(s) 294, 366, 659, 847, 1407, 1479, 1772, 1945
CspAI ACCGGT 2 cut(s) 368, 2570
CviQI GTAC 8 cut(s) 294, 366, 659, 847, 1407, 1479, 1772, 1945
DdeI CTNAG 5 cut(s) 545, 975, 1658, 2073, 2286
DinI GGCGCC 1 cut(s) 761
DpnI GATC 8 cut(s) 450, 702, 890, 1563, 1988, 2228, 2313, 2383
DpnII GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
EaeI YGGCCR 4 cut(s) 639, 1036, 1752, 2134
Eam1104I CTCTTC 2 cut(s) 345, 1458
EarI CTCTTC 2 cut(s) 345, 1458
Eco105I TACGTA 1 cut(s) 2778
Eco130I CCWWGG 2 cut(s) 2065, 2700
Eco147I AGGCCT 2 cut(s) 79, 2770
Eco31I GGTCTC 1 cut(s) 1218
Eco47I GGWCC 5 cut(s) 389, 462, 1129, 1502, 1575
Eco47III AGCGCT 1 cut(s) 956
Eco57I CTGAAG 1 cut(s) 2652
Eco72I CACGTG 1 cut(s) 2164
Eco88I CYCGRG 3 cut(s) 630, 1705, 1743
EcoNI CCTNNNNNAGG 1 cut(s) 2597
EcoO109I RGGNCCY 2 cut(s) 1167, 2265
EcoRI GAATTC 2 cut(s) 473, 1586
EcoRII CCWGG 4 cut(s) 218, 762, 1331, 2598
EcoT14I CCWWGG 2 cut(s) 2065, 2700
EcoT22I ATGCAT 2 cut(s) 862, 1960
EgeI GGCGCC 1 cut(s) 761
EheI GGCGCC 1 cut(s) 761
ErhI CCWWGG 2 cut(s) 2065, 2700
Esp3I CGTCTC 1 cut(s) 108
FaqI GGGAC 7 cut(s) 375, 1115, 1164, 1488, 2507, 2596, 2655
FauNDI CATATG 1 cut(s) 2757
FbaI TGATCA 2 cut(s) 448, 1561
FspBI CTAG 8 cut(s) 353, 623, 675, 1736, 1788, 2366, 2444, 2688
GlaI GCGC 8 cut(s) 179, 496, 761, 956, 1068, 1292, 1609, 2556
GsuI CTGGAG 2 cut(s) 270, 1383
HaeII RGCGCY 3 cut(s) 763, 958, 2558
HapII CCGG 5 cut(s) 56, 369, 387, 1500, 2571
HgaI GACGC 2 cut(s) 125, 1073
HhaI GCGC 8 cut(s) 180, 497, 762, 957, 1069, 1293, 1610, 2557
Hin1I GRCGYC 3 cut(s) 45, 117, 760
Hin6I GCGC 8 cut(s) 178, 495, 760, 955, 1067, 1291, 1608, 2555
HinP1I GCGC 8 cut(s) 178, 495, 760, 955, 1067, 1291, 1608, 2555
HindIII AAGCTT 1 cut(s) 2717
HinfI GANTC 8 cut(s) 65, 137, 799, 816, 1247, 1899, 1914, 2495
HpaII CCGG 5 cut(s) 56, 369, 387, 1500, 2571
HphI GGTGA 7 cut(s) 443, 581, 695, 1556, 1652, 1694, 2287
Hpy166II GTNNAC 7 cut(s) 582, 683, 1009, 1695, 1796, 2279, 2337
Hpy8I GTNNAC 7 cut(s) 582, 683, 1009, 1695, 1796, 2279, 2337
Hpy99I CGWCG 3 cut(s) 32, 50, 1067
HpyAV CCTTC 6 cut(s) 202, 799, 1315, 1897, 2071, 2781
HpyCH4III ACNGT 7 cut(s) 687, 707, 826, 1800, 1924, 2191, 2516
HpyCH4IV ACGT 4 cut(s) 45, 1727, 2163, 2777
HpyF10VI GCNNNNNNNGC 2 cut(s) 13, 2408
HpyF3I CTNAG 5 cut(s) 545, 975, 1658, 2073, 2286
HpySE526I ACGT 4 cut(s) 45, 1727, 2163, 2777
Hsp92I GRCGYC 3 cut(s) 45, 117, 760
HspAI GCGC 8 cut(s) 178, 495, 760, 955, 1067, 1291, 1608, 2555
KasI GGCGCC 1 cut(s) 759
Ksp22I TGATCA 2 cut(s) 448, 1561
Kzo9I GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
LmnI GCTCC 5 cut(s) 175, 785, 1288, 1883, 2069
Lsp1109I GCAGC 7 cut(s) 273, 638, 1386, 1751, 2209, 2423, 2433
MaeI CTAG 8 cut(s) 353, 623, 675, 1736, 1788, 2366, 2444, 2688
MaeII ACGT 4 cut(s) 45, 1727, 2163, 2777
MaeIII GTNAC 5 cut(s) 23, 520, 538, 1633, 1651
MalI GATC 8 cut(s) 450, 702, 890, 1563, 1988, 2228, 2313, 2383
MboI GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
MboII GAAGA 8 cut(s) 179, 332, 390, 1087, 1292, 1445, 1503, 1752
MhlI GDGCHC 2 cut(s) 9, 981
MlsI TGGCCA 4 cut(s) 641, 1038, 1754, 2136
MluNI TGGCCA 4 cut(s) 641, 1038, 1754, 2136
Mly113I GGCGCC 1 cut(s) 760
MlyI GAGTC 5 cut(s) 146, 808, 1256, 1893, 2489
MmeI TCCRAC 5 cut(s) 120, 921, 1230, 1789, 2019
Mox20I TGGCCA 4 cut(s) 641, 1038, 1754, 2136
Mph1103I ATGCAT 2 cut(s) 862, 1960
MroXI GAANNNNTTC 1 cut(s) 147
MscI TGGCCA 4 cut(s) 641, 1038, 1754, 2136
MseI TTAA 3 cut(s) 696, 2432, 2733
MslI CAYNNNNRTG 3 cut(s) 21, 2346, 2710
Msp20I TGGCCA 4 cut(s) 641, 1038, 1754, 2136
MspA1I CMGCKG 2 cut(s) 229, 1342
MspI CCGG 5 cut(s) 56, 369, 387, 1500, 2571
MspR9I CCNGG 4 cut(s) 220, 764, 1333, 2600
MvaI CCWGG 4 cut(s) 220, 764, 1333, 2600
MvnI CGCG 7 cut(s) 135, 180, 495, 1067, 1245, 1293, 1608
MwoI GCNNNNNNNGC 2 cut(s) 13, 2408
NarI GGCGCC 1 cut(s) 760
NcoI CCATGG 1 cut(s) 2065
NdeI CATATG 1 cut(s) 2757
NdeII GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
NlaIV GGNNCC 8 cut(s) 60, 391, 761, 787, 1131, 1504, 1859, 1885
NmuCI GTSAC 3 cut(s) 23, 520, 1633
NsiI ATGCAT 2 cut(s) 862, 1960
NspI RCATGY 8 cut(s) 539, 926, 1106, 1627, 1652, 2024, 2204, 2709
PaeI GCATGC 5 cut(s) 926, 1106, 2024, 2204, 2709
PaeR7I CTCGAG 2 cut(s) 630, 1743
PceI AGGCCT 2 cut(s) 79, 2770
PciI ACATGT 2 cut(s) 535, 1648
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 3 cut(s) 65, 816, 1914
PinAI ACCGGT 2 cut(s) 368, 2570
PleI GAGTC 5 cut(s) 145, 807, 1255, 1893, 2489
PluTI GGCGCC 1 cut(s) 763
PmaCI CACGTG 1 cut(s) 2164
PmlI CACGTG 1 cut(s) 2164
PpsI GAGTC 5 cut(s) 145, 807, 1255, 1893, 2489
Ppu21I YACGTR 2 cut(s) 2164, 2778
PscI ACATGT 2 cut(s) 535, 1648
PsiI TTATAA 2 cut(s) 1821, 2794
Psp1406I AACGTT 1 cut(s) 1727
Psp6I CCWGG 4 cut(s) 218, 762, 1331, 2598
PspCI CACGTG 1 cut(s) 2164
PspGI CCWGG 4 cut(s) 218, 762, 1331, 2598
PspN4I GGNNCC 8 cut(s) 60, 391, 761, 787, 1131, 1504, 1859, 1885
PspPI GGNCC 7 cut(s) 389, 462, 1129, 1167, 1502, 1575, 2265
PspXI VCTCGAGB 2 cut(s) 630, 1743
PsrI GAACNNNNNNTAC 2 cut(s) 2343, 2375
PstI CTGCAG 2 cut(s) 843, 1941
RsaI GTAC 8 cut(s) 295, 367, 660, 848, 1408, 1480, 1773, 1946
RsaNI GTAC 8 cut(s) 294, 366, 659, 847, 1407, 1479, 1772, 1945
RseI CAYNNNNRTG 3 cut(s) 21, 2346, 2710
SaqAI TTAA 3 cut(s) 696, 2432, 2733
Sau3AI GATC 8 cut(s) 448, 700, 888, 1561, 1986, 2226, 2311, 2381
Sau96I GGNCC 7 cut(s) 389, 462, 1129, 1167, 1502, 1575, 2265
ScaI AGTACT 1 cut(s) 1480
SchI GAGTC 5 cut(s) 146, 808, 1256, 1893, 2489
ScrFI CCNGG 4 cut(s) 220, 764, 1333, 2600
SduI GDGCHC 2 cut(s) 9, 981
SfcI CTRYAG 5 cut(s) 375, 839, 1488, 1937, 2512
SfoI GGCGCC 1 cut(s) 761
Sfr274I CTCGAG 2 cut(s) 630, 1743
SinI GGWCC 5 cut(s) 389, 462, 1129, 1502, 1575
SlaI CTCGAG 2 cut(s) 630, 1743
SmiMI CAYNNNNRTG 3 cut(s) 21, 2346, 2710
SmlI CTYRAG 4 cut(s) 8, 592, 630, 1743
SmoI CTYRAG 4 cut(s) 8, 592, 630, 1743
SnaBI TACGTA 1 cut(s) 2778
SpeI ACTAGT 1 cut(s) 352
SphI GCATGC 5 cut(s) 926, 1106, 2024, 2204, 2709
SseBI AGGCCT 2 cut(s) 79, 2770
SsiI CCGC 9 cut(s) 180, 224, 227, 1293, 1337, 1340, 2120, 2534, 2727
SspDI GGCGCC 1 cut(s) 759
SspMI CTAG 8 cut(s) 353, 623, 675, 1736, 1788, 2366, 2444, 2688
StuI AGGCCT 2 cut(s) 79, 2770
StyD4I CCNGG 4 cut(s) 218, 762, 1331, 2598
StyI CCWWGG 2 cut(s) 2065, 2700
TaaI ACNGT 7 cut(s) 687, 707, 826, 1800, 1924, 2191, 2516
TaiI ACGT 4 cut(s) 48, 1730, 2166, 2780
TaqII GACCGA 2 cut(s) 479, 1592
TatI WGTACW 3 cut(s) 846, 1478, 1944
TauI GCSGC 7 cut(s) 226, 229, 1339, 1342, 2123, 2536, 2729
TfiI GAWTC 3 cut(s) 65, 816, 1914
Tru1I TTAA 3 cut(s) 696, 2432, 2733
Tru9I TTAA 3 cut(s) 696, 2432, 2733
TscAI CASTG 3 cut(s) 831, 1638, 2521
TseFI GTSAC 3 cut(s) 23, 520, 1633
TseI GCWGC 7 cut(s) 286, 626, 1399, 1739, 2197, 2411, 2421
Tsp45I GTSAC 3 cut(s) 23, 520, 1633
TspDTI ATGAA 7 cut(s) 144, 765, 1071, 1254, 1863, 2169, 2364
TspGWI ACGGA 8 cut(s) 371, 798, 994, 1484, 1896, 2394, 2494, 2707
TspRI CASTG 3 cut(s) 831, 1638, 2521
VpaK11BI GGWCC 5 cut(s) 389, 462, 1129, 1502, 1575
XagI CCTNNNNNAGG 1 cut(s) 2597
XapI RAATTY 4 cut(s) 473, 1586, 1814, 2322
XceI RCATGY 8 cut(s) 539, 926, 1106, 1627, 1652, 2024, 2204, 2709
XcmI CCANNNNNNNNNTGG 2 cut(s) 226, 1339
XhoI CTCGAG 2 cut(s) 630, 1743
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 8 cut(s) 353, 623, 675, 1736, 1788, 2366, 2444, 2688
ZraI GACGTC 1 cut(s) 46
ZrmI AGTACT 1 cut(s) 1480
Zsp2I ATGCAT 2 cut(s) 862, 1960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.