Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010921

Physical Location & Seq

Genomic Coordinates Forward (+)

28542563 .. 28547043

Gene Structure 4481 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1197400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGCCATACGAAACTGAAAAAACGAAGAGTTCAGCGGCAGCGGCGGCGGCAGGGCATGGTATGATGGAGATAGAAGCGGGAAAGCCCTCAGGAAACGGGGTTGTGGCCGGAGGGCTGAGCCATCTGAGTGAGACTCTGTGGAGGGAGAAGACAAGTTTGGAGATTGTGGGAGATGTGTCTGCAAGGCTGACGTGGAAGGATCTGACGGTGATGGTGACTCTGAACAACGGCGAGACGCAGAAGGTGTTGGAGGGTTTGACTGGTTACGCTGAGCCTGGAACTTTCACGGCTCTCATGGGGCCTTCTGGTTCTGGGAAGTCTACTTTGCTTGATGCTCTGTCGAGTCGGTTGGCCGCCAATGCTTTTCTTTCTGGTACTATTCTGCTCAATGGCCGCAAACGGAAGCTCTCTTTTGGCACTGCTGCCTATGTGACCCAAGATGACAATTTGATTGGAACTTTAACGGTGCGAGAGACGATTTCTTATTCGGCAAGGCTAAGGCTGCCGGATAAGATGGCTTGGTCGGAAAAGCGGGCACTGGTTGAGAGTACAATCATAGAGATGGGTCTGCAGGATTGCGCCGACACAGTTATTGGAAATTGGCATTTGCGTGGGATCAGTGGGGGAGAGAAGAGAAGGGTCAGCATTGCTCTTGAAATCTTGACGAGGCCTAGATTGCTCTTCCTGGATGAGCCAACAAGCGGACTTGATAGTGCTTCGGCGTTTTTCGTGACTCAGACATTGCGTGCCCTGGCACGAGACGGAAGGACTGTCATAGCCTCAATTCACCAGCCCAGTAGTGAAGTCTTTGAACTGTTTGATCAACTGTACTTGCTTTCTAGTGGCAAAACAGTCTATTTTGGTCAGGCGGCAGACGCATATGGGTTCTTTGCACAAGCCGGCTTTCCGTGCCCTGCTTTGAGGAACCCATCTGATCATTTTCTTAGATGCATAAACTCCGACTTCGACAAAGTAAAGGCCACACTGAAAGGGTCCATGGAACTCAGGTTTGAGGCAAGTGATGACCCGTTGGAGAAGATTACCACTGCTGAAGCTATAAGAGTTCTTATTGACTCCTATCGTTCTTCTCAATACTCGTACGCAGCTAGAGAAAAAGTTGAGGAGATATCGAAAGTTAAAGGAACCGTGCTAGATTCTGGAGGAAGTCAGGCTAGTTTCTTTATGCAGGCCTTCCAATTGTCCAAGCGTTCGTTTATCAATATGTCGAGGGACTTTGGATACTACTGGCTCAGGCTCGTCATTTACATTGTCGTCACAGTTTGTATCGGAACCATTTATTTGAATGTGGGAACGGGTTACAGTTCGATTCTGGCACGGGGTTCGTGTGCATCATTCGTCTTTGGTTTTGTCACGTTCATGTCAATTGGCGGGTTTCCTTCCTTCGTAGAGGATATGAAGGTTTTCCAAAGAGAGAGGCTGAATGGCCACTACGGCGTTACTGCATTTGTCATCAGCAACACGCTCTCTGCAATGCCATTCCTGATACTGATTACCTTTCTCGCCGGAACTGTTTGTTACTTCATGGTCCGGCTCCACCCGGGCTTTGAGCATTATCTCTTCTTTGTGTTGTGCCTTTATGCAAGTGTCACCGTCGTTGAGAGCTTGATGATGGCGATAGCCAGCATCGTCCCCAACTTCCTCATGGGCATTATCATAGGGGCTGGAATTCAGGGTATCTTCATGCTAGTCTCCGGGTACTTCAGGCTCCCGAATGACATCCCAAAACCCGTCTGGCGTTACCCTATGTCATACATCAGCTTCCACTTCTGGGCTTTACAGGGACAATACCAGAATGACCTGAGCGGCTTATTGTTCGACAACCAGACACCGGACCTTCCTAAGATTCCCGGCGAGTACATCCTGGAAAATGTGTTCCAGATTAACACAGCCAGATCGAAATGGATAGACCTGAGTGTGATCTTGAGCATGATTGTCGTGTACCGGATCATCTTCTTCATCATGATCAAGTTCAGCGAGGATGTGACGCCGTGGATTCGAGGTTACATGGCGAGGCGAAGAATACAACAGAAGAATGGAAACCAGAACACAACAGTTGCTCCCGATGGCCTCACACAGTCACCTTCTTTGAGGAACCATGCAAGAAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0005215 GO:0005319 GO:0005342 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006950 GO:0006970 GO:0007275 GO:0007389 GO:0008028 GO:0008150 GO:0008509 GO:0008514 GO:0009611 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009897 GO:0009965 GO:0009986 GO:0010016 GO:0010033 GO:0010051 GO:0010154 GO:0010222 GO:0010588 GO:0010876 GO:0015075 GO:0015245 GO:0015318 GO:0015399 GO:0015405 GO:0015711 GO:0015718 GO:0015849 GO:0015908 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022414 GO:0022804 GO:0022857 GO:0031224 GO:0032501 GO:0032502 GO:0033036 GO:0033993 GO:0034220 GO:0042221 GO:0042623 GO:0042626 GO:0042802 GO:0042803 GO:0043492 GO:0044425 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0046983 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0061458 GO:0071702 GO:0071944 GO:0080051 GO:0090698 GO:0097305 GO:0098552 GO:0098656 GO:0099402 GO:1901700 GO:1903825 GO:1905039 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

710

Amino Acids

78.59

Weight (kDa)

8.72

Isoelectric Point (pI)

35.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	84 - 234	5.1e-25	ABC transporter
AAA_29	PF13555	95 - 120	2.2e-06	P-loop containing region of AAA domain
ABC2_membrane	PF01061	396 - 605	2.1e-38	ABC-2 type transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010921)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17840
fragaria_vesca	FvH4_6g06950 FvH4_6g06950
malus_domestica	MD04G1197400.v1.1 MD12G1210200.v1.1
prunus_persica	Prupe.6G317800_v2.0.a1 Prupe.6G317800_v2.0.a1
pyrus_communis	pycom04g17460 pycom12g19760
rosa_chinensis	RchiOBHm_Chr3g0456491
rosa_laevigata	RLG00000025301
rosa_multiflora	Rmu_sc0002607.1_g000010 Rmu_ssc0000446.1_g000010
rosa_roxburghii	Rroxscaffold_6G00422710
rosa_rugosa	Rorug03G0013400
rosa_samantha	Rh3AG073800 Rh3CG075200 Rh3DG077000
rosa_wichuraiana	Rw0G012260 Rw3G006330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	1824
AccI	GTMKAC	1 cut(s)	320
AclWI	GGATC	3 cut(s)	207, 623, 1973
AcoI	YGGCCR	4 cut(s)	105, 351, 391, 1444
AcsI	RAATTY	1 cut(s)	1686
AcuI	CTGAAG	2 cut(s)	1071, 1705
AcyI	GRCGYC	1 cut(s)	2006
AfaI	GTAC	7 cut(s)	376, 550, 830, 1100, 1718, 1877, 1961
AfiI	CCNNNNNNNGG	3 cut(s)	701, 1789, 1849
AgsI	TTSAA	3 cut(s)	656, 812, 1303
AjiI	CACGTC	1 cut(s)	192
AjnI	CCWGG	4 cut(s)	274, 684, 750, 1881
AjuI	GAANNNNNNNTTGG	2 cut(s)	140, 172
AloI	GAACNNNNNNTCC	2 cut(s)	1877, 1909
AluBI	AGCT	5 cut(s)	406, 1055, 1106, 1623, 1779
AluI	AGCT	5 cut(s)	406, 1055, 1106, 1623, 1779
Alw26I	GTCTC	5 cut(s)	125, 227, 467, 753, 1714
AlwI	GGATC	3 cut(s)	207, 623, 1973
Ama87I	CYCGRG	1 cut(s)	1558
AoxI	GGCC	9 cut(s)	105, 299, 351, 391, 668, 978, 1188, 1444, 2086
ApeKI	GCWGC	4 cut(s)	38, 422, 502, 1103
ApoI	RAATTY	1 cut(s)	1686
Asp700I	GAANNNNTTC	1 cut(s)	1421
AspLEI	GCGC	1 cut(s)	581
AspS9I	GGNCC	4 cut(s)	299, 993, 1546, 1852
AsuC2I	CCSGG	4 cut(s)	1559, 1560, 1714, 1869
AsuHPI	GGTGA	5 cut(s)	220, 226, 779, 1600, 2091
AvaI	CYCGRG	1 cut(s)	1558
AvaII	GGWCC	3 cut(s)	993, 1546, 1852
AxyI	CCTNAGG	1 cut(s)	88
BaeGI	GKGCMC	3 cut(s)	538, 751, 914
BalI	TGGCCA	1 cut(s)	1446
BauI	CACGAG	1 cut(s)	756
BbsI	GAAGAC	1 cut(s)	155
BbvI	GCAGC	4 cut(s)	50, 409, 489, 1115
BccI	CCATC	8 cut(s)	58, 129, 205, 508, 556, 937, 1624, 2078
BceAI	ACGGC	4 cut(s)	244, 303, 1468, 1993
BcgI	CGANNNNNNTGC	4 cut(s)	1323, 1357, 1936, 1970
BciT130I	CCWGG	4 cut(s)	276, 686, 752, 1883
BciVI	GTATCC	1 cut(s)	1232
BclI	TGATCA	3 cut(s)	820, 934, 1983
BcnI	CCSGG	4 cut(s)	1559, 1560, 1714, 1869
BcoDI	GTCTC	5 cut(s)	125, 227, 467, 753, 1714
BfaI	CTAG	6 cut(s)	672, 840, 1107, 1151, 1173, 1706
BfmI	CTRYAG	1 cut(s)	569
BfuI	GTATCC	1 cut(s)	1232
BglI	GCCNNNNNGGC	1 cut(s)	1452
BlpI	GCTNAGC	2 cut(s)	116, 270
Bme1390I	CCNGG	8 cut(s)	276, 686, 752, 1559, 1560, 1714, 1869, 1883
Bme18I	GGWCC	3 cut(s)	993, 1546, 1852
BmeT110I	CYCGRG	1 cut(s)	1558
BmgBI	CACGTC	1 cut(s)	192
BmgT120I	GGNCC	4 cut(s)	299, 993, 1546, 1852
BmiI	GGNNCC	8 cut(s)	300, 926, 994, 1144, 1291, 1553, 1727, 2114
BmrFI	CCNGG	8 cut(s)	276, 686, 752, 1559, 1560, 1714, 1869, 1883
BmrI	ACTGGG	1 cut(s)	789
BmsI	GCATC	4 cut(s)	322, 938, 1358, 1653
BmuI	ACTGGG	1 cut(s)	789
BpiI	GAAGAC	1 cut(s)	155
BplI	GAGNNNNNCTC	2 cut(s)	118, 150
BpmI	CTGGAG	1 cut(s)	1179
Bpu10I	CCTNAGC	3 cut(s)	497, 1250, 1820
Bpu1102I	GCTNAGC	2 cut(s)	116, 270
BpuEI	CTTGAG	1 cut(s)	1963
BpuMI	CCSGG	4 cut(s)	1559, 1560, 1714, 1869
BsaHI	GRCGYC	1 cut(s)	2006
BsaJI	CCNNGG	4 cut(s)	750, 996, 1558, 2009
BsaWI	WCCGGW	2 cut(s)	1849, 1962
BsaXI	ACNNNNNCTCC	2 cut(s)	2062, 2092
Bsc4I	CCNNNNNNNGG	3 cut(s)	701, 1789, 1849
Bse118I	RCCGGY	1 cut(s)	899
Bse1I	ACTGG	4 cut(s)	265, 543, 795, 1250
Bse21I	CCTNAGG	1 cut(s)	88
Bse3DI	GCAATG	3 cut(s)	645, 740, 1497
BseBI	CCWGG	4 cut(s)	276, 686, 752, 1883
BseDI	CCNNGG	4 cut(s)	750, 996, 1558, 2009
BseGI	GGATG	4 cut(s)	694, 1737, 1878, 2005
BseLI	CCNNNNNNNGG	3 cut(s)	701, 1789, 1849
BseMI	GCAATG	3 cut(s)	645, 740, 1497
BseMII	CTCAG	9 cut(s)	102, 107, 116, 261, 749, 1018, 1264, 1811, 1922
BseNI	ACTGG	4 cut(s)	265, 543, 795, 1250
BseRI	GAGGAG	1 cut(s)	1136
BseSI	GKGCMC	3 cut(s)	538, 751, 914
BseXI	GCAGC	4 cut(s)	50, 409, 489, 1115
BshFI	GGCC	9 cut(s)	107, 301, 353, 393, 670, 980, 1190, 1446, 2088
BsiHKCI	CYCGRG	1 cut(s)	1558
BsiWI	CGTACG	1 cut(s)	1098
BslFI	GGGAC	3 cut(s)	1244, 1634, 1815
BslI	CCNNNNNNNGG	3 cut(s)	701, 1789, 1849
BsmAI	GTCTC	5 cut(s)	125, 227, 467, 753, 1714
BsmBI	CGTCTC	3 cut(s)	227, 467, 753
BsmFI	GGGAC	3 cut(s)	1244, 1634, 1815
BsnI	GGCC	9 cut(s)	107, 301, 353, 393, 670, 980, 1190, 1446, 2088
BsoBI	CYCGRG	1 cut(s)	1558
Bsp1286I	GDGCHC	3 cut(s)	538, 751, 914
Bsp143I	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
Bsp1720I	GCTNAGC	2 cut(s)	116, 270
Bsp19I	CCATGG	1 cut(s)	996
BspANI	GGCC	9 cut(s)	107, 301, 353, 393, 670, 980, 1190, 1446, 2088
BspCNI	CTCAG	9 cut(s)	101, 108, 117, 262, 748, 1017, 1263, 1812, 1923
BspHI	TCATGA	1 cut(s)	1980
BspLI	GGNNCC	8 cut(s)	300, 926, 994, 1144, 1291, 1553, 1727, 2114
BspMAI	CTGCAG	1 cut(s)	573
BspPI	GGATC	3 cut(s)	207, 623, 1973
BspQI	GCTCTTC	1 cut(s)	686
BsrBI	CCGCTC	1 cut(s)	1824
BsrDI	GCAATG	3 cut(s)	645, 740, 1497
BsrFI	RCCGGY	1 cut(s)	899
BsrI	ACTGG	4 cut(s)	265, 543, 795, 1250
BssAI	RCCGGY	1 cut(s)	899
BssECI	CCNNGG	4 cut(s)	750, 996, 1558, 2009
BssMI	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
BssNI	GRCGYC	1 cut(s)	2006
BssSI	CACGAG	1 cut(s)	756
BssT1I	CCWWGG	1 cut(s)	996
Bst2BI	CACGAG	1 cut(s)	756
Bst2UI	CCWGG	4 cut(s)	276, 686, 752, 1883
Bst6I	CTCTTC	4 cut(s)	20, 626, 686, 1583
BstACI	GRCGYC	1 cut(s)	2006
BstC8I	GCNNGC	5 cut(s)	534, 747, 901, 1188, 1642
BstDSI	CCRYGG	2 cut(s)	996, 2009
BstF5I	GGATG	4 cut(s)	694, 1737, 1878, 2005
BstHHI	GCGC	1 cut(s)	581
BstKTI	GATC	8 cut(s)	202, 618, 823, 937, 1916, 1941, 1968, 1986
BstMAI	GTCTC	5 cut(s)	125, 227, 467, 753, 1714
BstMBI	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
BstMWI	GCNNNNNNNGC	9 cut(s)	41, 44, 47, 359, 502, 676, 875, 909, 1452
BstNI	CCWGG	4 cut(s)	276, 686, 752, 1883
BstSCI	CCNGG	8 cut(s)	274, 684, 750, 1557, 1558, 1712, 1867, 1881
BstSFI	CTRYAG	1 cut(s)	569
BstSLI	GKGCMC	3 cut(s)	538, 751, 914
BstV1I	GCAGC	4 cut(s)	50, 409, 489, 1115
BstV2I	GAAGAC	1 cut(s)	155
BstX2I	RGATCY	1 cut(s)	199
BstYI	RGATCY	1 cut(s)	199
Bsu36I	CCTNAGG	1 cut(s)	88
BsuI	GTATCC	1 cut(s)	1232
BsuRI	GGCC	9 cut(s)	107, 301, 353, 393, 670, 980, 1190, 1446, 2088
BtgI	CCRYGG	2 cut(s)	996, 2009
BtrI	CACGTC	1 cut(s)	192
BtsCI	GGATG	4 cut(s)	694, 1737, 1878, 2005
BtsI	GCAGTG	2 cut(s)	417, 1044
BtsIMutI	CAGTG	5 cut(s)	417, 536, 625, 983, 1044
Cac8I	GCNNGC	5 cut(s)	534, 747, 901, 1188, 1642
CciI	TCATGA	1 cut(s)	1980
CfoI	GCGC	1 cut(s)	581
Cfr10I	RCCGGY	1 cut(s)	899
Cfr13I	GGNCC	4 cut(s)	299, 993, 1546, 1852
Cfr9I	CCCGGG	1 cut(s)	1558
CseI	GACGC	3 cut(s)	244, 884, 2014
Csp6I	GTAC	7 cut(s)	375, 549, 829, 1099, 1717, 1876, 1960
CviQI	GTAC	7 cut(s)	375, 549, 829, 1099, 1717, 1876, 1960
DpnI	GATC	8 cut(s)	201, 617, 822, 936, 1915, 1940, 1967, 1985
DpnII	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
EaeI	YGGCCR	4 cut(s)	105, 351, 391, 1444
Eam1104I	CTCTTC	4 cut(s)	20, 626, 686, 1583
EarI	CTCTTC	4 cut(s)	20, 626, 686, 1583
Eco130I	CCWWGG	1 cut(s)	996
Eco147I	AGGCCT	2 cut(s)	670, 1190
Eco32I	GATATC	1 cut(s)	1128
Eco47I	GGWCC	3 cut(s)	993, 1546, 1852
Eco57I	CTGAAG	2 cut(s)	1071, 1705
Eco81I	CCTNAGG	1 cut(s)	88
Eco88I	CYCGRG	1 cut(s)	1558
EcoO109I	RGGNCCY	1 cut(s)	299
EcoRI	GAATTC	1 cut(s)	1686
EcoRII	CCWGG	4 cut(s)	274, 684, 750, 1881
EcoRV	GATATC	1 cut(s)	1128
EcoT14I	CCWWGG	1 cut(s)	996
EcoT22I	ATGCAT	1 cut(s)	953
ErhI	CCWWGG	1 cut(s)	996
Esp3I	CGTCTC	3 cut(s)	227, 467, 753
FaqI	GGGAC	3 cut(s)	1244, 1634, 1815
FauI	CCCGC	3 cut(s)	70, 525, 1382
FauNDI	CATATG	1 cut(s)	880
FbaI	TGATCA	3 cut(s)	820, 934, 1983
FblI	GTMKAC	1 cut(s)	320
FokI	GGATG	4 cut(s)	701, 1724, 1865, 2012
FspBI	CTAG	6 cut(s)	672, 840, 1107, 1151, 1173, 1706
GlaI	GCGC	1 cut(s)	580
GsuI	CTGGAG	1 cut(s)	1179
HaeIII	GGCC	9 cut(s)	107, 301, 353, 393, 670, 980, 1190, 1446, 2088
HgaI	GACGC	3 cut(s)	244, 884, 2014
HhaI	GCGC	1 cut(s)	581
Hin1I	GRCGYC	1 cut(s)	2006
Hin6I	GCGC	1 cut(s)	579
HinP1I	GCGC	1 cut(s)	579
HinfI	GANTC	9 cut(s)	133, 217, 343, 733, 1073, 1154, 1327, 1864, 2014
HphI	GGTGA	5 cut(s)	220, 226, 779, 1600, 2091
Hpy166II	GTNNAC	2 cut(s)	321, 1960
Hpy188I	TCNGA	8 cut(s)	126, 204, 222, 526, 738, 934, 961, 1289
Hpy8I	GTNNAC	2 cut(s)	321, 1960
Hpy99I	CGWCG	1 cut(s)	1616
HpyCH4IV	ACGT	2 cut(s)	191, 1373
HpyF10VI	GCNNNNNNNGC	9 cut(s)	41, 44, 47, 359, 502, 676, 875, 909, 1452
HpySE526I	ACGT	2 cut(s)	191, 1373
Hsp92I	GRCGYC	1 cut(s)	2006
HspAI	GCGC	1 cut(s)	579
KroI	GCCGGC	1 cut(s)	899
KroNI	GCCGGC	1 cut(s)	901
Ksp22I	TGATCA	3 cut(s)	820, 934, 1983
Kzo9I	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
LguI	GCTCTTC	1 cut(s)	686
LmnI	GCTCC	3 cut(s)	1557, 1731, 2083
Lsp1109I	GCAGC	4 cut(s)	50, 409, 489, 1115
LweI	GCATC	4 cut(s)	322, 938, 1358, 1653
MaeI	CTAG	6 cut(s)	672, 840, 1107, 1151, 1173, 1706
MaeII	ACGT	2 cut(s)	191, 1373
MalI	GATC	8 cut(s)	201, 617, 822, 936, 1915, 1940, 1967, 1985
MbiI	CCGCTC	1 cut(s)	1824
MboI	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
MfeI	CAATTG	2 cut(s)	1196, 1383
MflI	RGATCY	1 cut(s)	199
MhlI	GDGCHC	3 cut(s)	538, 751, 914
MlsI	TGGCCA	1 cut(s)	1446
MluCI	AATT	6 cut(s)	445, 598, 783, 1196, 1383, 1686
MluNI	TGGCCA	1 cut(s)	1446
MlyI	GAGTC	5 cut(s)	127, 211, 352, 727, 1067
MmeI	TCCRAC	4 cut(s)	228, 504, 984, 1011
Mox20I	TGGCCA	1 cut(s)	1446
Mph1103I	ATGCAT	1 cut(s)	953
MroNI	GCCGGC	1 cut(s)	899
MroXI	GAANNNNTTC	1 cut(s)	1421
MscI	TGGCCA	1 cut(s)	1446
MseI	TTAA	3 cut(s)	461, 1137, 1902
MslI	CAYNNNNRTG	4 cut(s)	126, 560, 609, 1376
Msp20I	TGGCCA	1 cut(s)	1446
MspA1I	CMGCKG	2 cut(s)	35, 41
MspR9I	CCNGG	8 cut(s)	276, 686, 752, 1559, 1560, 1714, 1869, 1883
MunI	CAATTG	2 cut(s)	1196, 1383
MvaI	CCWGG	4 cut(s)	276, 686, 752, 1883
MwoI	GCNNNNNNNGC	9 cut(s)	41, 44, 47, 359, 502, 676, 875, 909, 1452
NaeI	GCCGGC	1 cut(s)	901
NciI	CCSGG	4 cut(s)	1559, 1560, 1714, 1869
NcoI	CCATGG	1 cut(s)	996
NdeI	CATATG	1 cut(s)	880
NdeII	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
NgoMIV	GCCGGC	1 cut(s)	899
NlaIV	GGNNCC	8 cut(s)	300, 926, 994, 1144, 1291, 1553, 1727, 2114
NmuCI	GTSAC	8 cut(s)	214, 430, 730, 1273, 1369, 1606, 2002, 2097
NsiI	ATGCAT	1 cut(s)	953
PagI	TCATGA	1 cut(s)	1980
PceI	AGGCCT	2 cut(s)	670, 1190
PciSI	GCTCTTC	1 cut(s)	686
PdiI	GCCGGC	1 cut(s)	901
PdmI	GAANNNNTTC	1 cut(s)	1421
PfeI	GAWTC	4 cut(s)	1154, 1327, 1864, 2014
Pfl23II	CGTACG	1 cut(s)	1098
PfoI	TCCNGGA	2 cut(s)	684, 1881
PleI	GAGTC	5 cut(s)	127, 211, 351, 727, 1067
PpsI	GAGTC	5 cut(s)	127, 211, 351, 727, 1067
Psp6I	CCWGG	4 cut(s)	274, 684, 750, 1881
PspGI	CCWGG	4 cut(s)	274, 684, 750, 1881
PspLI	CGTACG	1 cut(s)	1098
PspN4I	GGNNCC	8 cut(s)	300, 926, 994, 1144, 1291, 1553, 1727, 2114
PspPI	GGNCC	4 cut(s)	299, 993, 1546, 1852
PstI	CTGCAG	1 cut(s)	573
PsuI	RGATCY	1 cut(s)	199
RsaI	GTAC	7 cut(s)	376, 550, 830, 1100, 1718, 1877, 1961
RsaNI	GTAC	7 cut(s)	375, 549, 829, 1099, 1717, 1876, 1960
RseI	CAYNNNNRTG	4 cut(s)	126, 560, 609, 1376
SapI	GCTCTTC	1 cut(s)	686
SaqAI	TTAA	3 cut(s)	461, 1137, 1902
Sau3AI	GATC	8 cut(s)	199, 615, 820, 934, 1913, 1938, 1965, 1983
Sau96I	GGNCC	4 cut(s)	299, 993, 1546, 1852
SchI	GAGTC	5 cut(s)	127, 211, 352, 727, 1067
ScrFI	CCNGG	8 cut(s)	276, 686, 752, 1559, 1560, 1714, 1869, 1883
SduI	GDGCHC	3 cut(s)	538, 751, 914
SfaNI	GCATC	4 cut(s)	322, 938, 1358, 1653
SfcI	CTRYAG	1 cut(s)	569
SinI	GGWCC	3 cut(s)	993, 1546, 1852
SmaI	CCCGGG	1 cut(s)	1560
SmiMI	CAYNNNNRTG	4 cut(s)	126, 560, 609, 1376
SmlI	CTYRAG	1 cut(s)	1942
SmoI	CTYRAG	1 cut(s)	1942
Sse9I	AATT	6 cut(s)	445, 598, 783, 1196, 1383, 1686
SseBI	AGGCCT	2 cut(s)	670, 1190
SspMI	CTAG	6 cut(s)	672, 840, 1107, 1151, 1173, 1706
StuI	AGGCCT	2 cut(s)	670, 1190
StyD4I	CCNGG	8 cut(s)	274, 684, 750, 1557, 1558, 1712, 1867, 1881
StyI	CCWWGG	1 cut(s)	996
TaiI	ACGT	2 cut(s)	194, 1376
TaqI	TCGA	8 cut(s)	341, 966, 1130, 1226, 1325, 1836, 1916, 2017
TasI	AATT	6 cut(s)	445, 598, 783, 1196, 1383, 1686
TatI	WGTACW	3 cut(s)	548, 828, 1875
TauI	GCSGC	8 cut(s)	38, 44, 47, 50, 356, 396, 872, 1827
TfiI	GAWTC	4 cut(s)	1154, 1327, 1864, 2014
Tru1I	TTAA	3 cut(s)	461, 1137, 1902
Tru9I	TTAA	3 cut(s)	461, 1137, 1902
TscAI	CASTG	5 cut(s)	424, 543, 625, 990, 1051
TseFI	GTSAC	8 cut(s)	214, 430, 730, 1273, 1369, 1606, 2002, 2097
TseI	GCWGC	4 cut(s)	38, 422, 502, 1103
Tsp45I	GTSAC	8 cut(s)	214, 430, 730, 1273, 1369, 1606, 2002, 2097
TspDTI	ATGAA	5 cut(s)	1366, 1430, 1531, 1690, 1966
TspGWI	ACGGA	3 cut(s)	415, 777, 897
TspMI	CCCGGG	1 cut(s)	1558
TspRI	CASTG	5 cut(s)	424, 543, 625, 990, 1051
VpaK11BI	GGWCC	3 cut(s)	993, 1546, 1852
XapI	RAATTY	1 cut(s)	1686
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1660, 1749
XmaI	CCCGGG	1 cut(s)	1558
XmiI	GTMKAC	1 cut(s)	320
XmnI	GAANNNNTTC	1 cut(s)	1421
XspI	CTAG	6 cut(s)	672, 840, 1107, 1151, 1173, 1706
Zsp2I	ATGCAT	1 cut(s)	953

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.