Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010218

Physical Location & Seq

Genomic Coordinates Forward (+)

28569472 .. 28574148

Gene Structure 4677 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1197900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGTCGTTGAGTACTGGCGGCTCCATGTCCGCGGCGAGCAGCCTCAGCGAGATATCGGATATGGAGACGCTGAAACTCGGATTGGATCTTGTATCCGCTGCCAGGCGAAATATCGGGTTCCTGAGAACGGTGGCCGAGTCTCAGTGGCTTCATCAGAAGGGAACGGCTGTTGAAGCCGTTAGAAGGTACAATGAGCTCTGGATGCCGTTGGTTTCTGATCTGACGGCGGAGTCGGCGGCGGTTCCTGTGATTCATCCTCCTATAGACATTGAGTGGGTTTGGTTCTGCCACACTCTGAATCCGGTTTCTTACAGGCACTACTGTGAACAAAAATTCTCAAACTTGATAGGAAAAGCAACCATTTTCAACGAAGAGAACAAAGAGTATGCACTGATGAAGTGCAGAGAAATATGGATTAGAAGGTACCCAAATGAGCCGTTTGAGAACGAAGCTGATTCGGATTCAAATTCAGAAGTGAGGTTTTCGGAGGTAGCCGATGAAGAAGCAGAGCTTTTGGAGGAAGTGAAAAAGAATAGGTTTTTGTATTCCAAGTTTTCGGAGCCATACAGGTCTGAAATTGTGTATTTGATAGCAGCAAGGCAGAGGTACAAGAGGTTTCTGTTCATGGTGCAGAGGTCCTCCATTGATTTGGATTCTCCTTTGGTCCCTGCCTCTGATATTATGCTCATGTGGCTCACCCACCAGAGTTACCCAACAGTGTATGCTGAGGATTTGAAAGGGATGGAGGGTGATTTGGGGAAGGTGGTGACCGTGTGGGCCGCTGTGAAGGAAAAAGAGGTGGAAGAGACCAGGAAGCTTTGGGAGAGGACGTTTGATCAACCATATGAGAAAGCTGGTGGAGAAATAGCTTTAAAATTGGACCGGGGTGTCTCATTCAAGCCGCCTGTTTACTGGGAGGCATCTGATACAGATGTCAATACCAAATACAAGCCCATGCATCCCAGGTTCTTGCTTGAGGTCTGTGTGCTTGTCAGGCTTAGGGATAAAACAAAGGTAATGCAAGAGGATACAAAGCGCAATATCCTTCGTCTTCGGATGGTGAGGTGCCACAGGGAGTTAAAGCTTGAAAAACCCATTTCTGACTTTCCGTATGCAACGTGGCAAAAAGCTTGGCACCTCTACTGTGAGTTTGGCACCAAAGGAGTCGTACTTGAGCTTCGCCGGCGAGGTGGCTCCTGTTTTAAAGGAACTAGCGTGCAAGAGACTGTTACATTCCATTGGAATGATTTACTTAGAGCACCGTCTCTAACTTTGGAGAAAGAATATCAACAAGTGGATATCTTTGCTTCAATAACTCCACCTGTTCAAGCACCATACCTTTTGAAATGTGTGCCAGACCGTGTCACAGATGATTCAGGGGCAATGATATCAGATGTGATTCTGAGAATGAACCAGTATCGTCCCCAAGAAGGCCGGTGGTTGTCTCGCACTGTTCTTGATCATGCAGGGAGAGAGTGTTTTGTGATCCGAATAAGGATGGGAGAAGGATTTTGGAGAAGAGGAGGAGAAAAACCGTCAGCTGTGAAATGGGAGGATAGGATTATAGAGATTCGAGAAGGTTCTTGGTCTTATGTTGCTGGTTCCATTGGTAGAGCCCCTGCGAAACTGGTGGGAACAGCTATACCCAAAGAACCGCTGGAACAATGGACAGCTGCATGGAATTTTTCAACAGGTGATGAATTGATGATACAGTGGGACTTGTCATCATCGATATCAGGTCTCAGTTTTGGTATACAGAGTCAAGATGCAGAATCAACGGTTAAGCTGTTGAAAGGACGGAAAATGCAGTATCGAGTATGGAAAAAAAGGCCAGTGACCGAAGATGAAGATCGTCAATATGAGGAAGAAGGTGAAGAGGAAGAAGACGAAGACGAAGAAGAGGGGTTTTTAACGCTAGTCCGGTACACAGAAGATAATCCAAATGGAAGAGCAACAGCTCTCTTAAATTGGAAGCTATTGGTAGCTGAATTGCTGCCTGAAGAAGATGCAGTATTGGTGCTTCTTCTATCCATTTCGATACTAAGAAGTGTTTCGGAGATGAGAAAAGAAGATCTCGGATGTTTGCTGATTAGAAGAAGACTGAAGGAAGTAAAACTTGGGACTCGAGATTGGGCTTCGGTAGTACTTCACCCTTCCTCAAGTTCTTCCATTTCGTCACCTTACCTTCAACCTTGGTACTGGAATGCAAAGGTAATTATGGCATCAGAGGGTGCAGATCATTTTACTAGGCAACCGGGTATTAGTTATTCGCCAGAGGAAGGCGGCGATAAGTTGTACAAACGAGGGATACTAGCTGAGAGACGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GRDP-like

Protein Analysis

776

Amino Acids

89.33

Weight (kDa)

5.75

Isoelectric Point (pI)

53.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GRDP-like	PF07173	22 - 105	5.2e-06	Glycine-rich domain-containing protein-like
GRDP-like	PF07173	103 - 244	2e-40	Glycine-rich domain-containing protein-like
GRDP_C	PF25335	485 - 663	2e-50	Glycine Rich Domain Protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56230 AT1G56230 AT1G56230 AT1G56230
fragaria_vesca	FvH4_6g06870
malus_domestica	MD04G1197900.v1.1
prunus_persica	Prupe.6G318400_v2.0.a1 Prupe.6G318400_v2.0.a1
pyrus_communis	pycom04g17510
rosa_chinensis	RchiOBHm_Chr3g0456361
rosa_laevigata	RLG00000025309
rosa_multiflora	Rmu_sc0002607.1_g000019 Rmu_sc0018054.1_g000001
rosa_roxburghii	Rroxscaffold_6G00422780
rosa_rugosa	Rorug03G0012700 Rorug03G0012700
rosa_samantha	Rh3AG072900 Rh3BG075000 Rh3CG074600 Rh3DG076400
rosa_wichuraiana	Rw3G006260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	229, 887
Acc65I	GGTACC	1 cut(s)	423
AccB1I	GGYRCC	4 cut(s)	423, 1065, 1134, 1154
AccI	GTMKAC	1 cut(s)	1753
AccII	CGCG	1 cut(s)	32
AclWI	GGATC	2 cut(s)	93, 1480
AcoI	YGGCCR	1 cut(s)	132
AcsI	RAATTY	3 cut(s)	332, 466, 1681
AcuI	CTGAAG	2 cut(s)	2019, 2123
AfaI	GTAC	9 cut(s)	13, 188, 425, 608, 1170, 1925, 2145, 2198, 2297
AfiI	CCNNNNNNNGG	5 cut(s)	102, 127, 183, 1430, 2279
AjnI	CCWGG	3 cut(s)	101, 809, 962
AjuI	GAANNNNNNNTTGG	4 cut(s)	65, 97, 2100, 2132
AleI	CACNNNNGTG	1 cut(s)	321
Alw21I	GWGCWC	2 cut(s)	198, 1261
Alw26I	GTCTC	9 cut(s)	59, 144, 800, 895, 1217, 1269, 1449, 1745, 2314
AlwI	GGATC	2 cut(s)	93, 1480
Ama87I	CYCGRG	1 cut(s)	2124
AoxI	GGCC	4 cut(s)	132, 777, 1432, 1829
ApeKI	GCWGC	5 cut(s)	39, 98, 593, 1673, 1993
ApoI	RAATTY	3 cut(s)	332, 466, 1681
Asp700I	GAANNNNTTC	1 cut(s)	2050
Asp718I	GGTACC	1 cut(s)	423
AspLEI	GCGC	1 cut(s)	1038
AspS9I	GGNCC	4 cut(s)	636, 664, 777, 880
AsuC2I	CCSGG	2 cut(s)	884, 2256
AsuHPI	GGTGA	8 cut(s)	688, 761, 778, 1072, 1706, 1883, 2141, 2169
AvaI	CYCGRG	1 cut(s)	2124
AvaII	GGWCC	3 cut(s)	636, 664, 880
BaeI	ACNNNNGTAYC	2 cut(s)	918, 951
BanI	GGYRCC	4 cut(s)	423, 1065, 1134, 1154
BanII	GRGCYC	2 cut(s)	198, 1618
BarI	GAAGNNNNNNTAC	4 cut(s)	1161, 1193, 2004, 2036
BbsI	GAAGAC	4 cut(s)	1043, 1890, 1896, 2104
Bbv12I	GWGCWC	2 cut(s)	198, 1261
BbvCI	CCTCAGC	2 cut(s)	44, 726
BbvI	GCAGC	5 cut(s)	51, 85, 605, 1660, 1980
BccI	CCATC	3 cut(s)	736, 1051, 1492
BceAI	ACGGC	5 cut(s)	161, 180, 190, 240, 421
BciT130I	CCWGG	3 cut(s)	103, 811, 964
BciVI	GTATCC	3 cut(s)	103, 1021, 2301
BclI	TGATCA	2 cut(s)	835, 1459
BcnI	CCSGG	2 cut(s)	884, 2256
BcoDI	GTCTC	9 cut(s)	59, 144, 800, 895, 1217, 1269, 1449, 1745, 2314
BfaI	CTAG	4 cut(s)	1212, 1916, 2247, 2312
BfmI	CTRYAG	1 cut(s)	261
BfuI	GTATCC	3 cut(s)	103, 1021, 2301
BglII	AGATCT	1 cut(s)	2071
BmcAI	AGTACT	2 cut(s)	13, 2145
Bme1390I	CCNGG	5 cut(s)	103, 811, 884, 964, 2256
Bme18I	GGWCC	3 cut(s)	636, 664, 880
BmeT110I	CYCGRG	1 cut(s)	2124
BmgT120I	GGNCC	4 cut(s)	636, 664, 777, 880
BmrFI	CCNGG	5 cut(s)	103, 811, 884, 964, 2256
BmrI	ACTGGG	1 cut(s)	922
BmsI	GCATC	6 cut(s)	192, 929, 967, 1756, 1996, 2231
BmuI	ACTGGG	1 cut(s)	922
BpiI	GAAGAC	4 cut(s)	1043, 1890, 1896, 2104
Bpu10I	CCTNAGC	3 cut(s)	44, 726, 998
BpuEI	CTTGAG	3 cut(s)	995, 1193, 2143
BpuMI	CCSGG	2 cut(s)	884, 2256
Bsa29I	ATCGAT	1 cut(s)	1730
BsaBI	GATNNNNATC	1 cut(s)	1848
BsaI	GGTCTC	2 cut(s)	800, 1745
BsaJI	CCNNGG	4 cut(s)	30, 883, 962, 2192
BsaWI	WCCGGW	2 cut(s)	301, 1920
BsaXI	ACNNNNNCTCC	4 cut(s)	815, 845, 1462, 1492
Bsc4I	CCNNNNNNNGG	5 cut(s)	102, 127, 183, 1430, 2279
Bse118I	RCCGGY	2 cut(s)	1182, 1434
Bse1I	ACTGG	6 cut(s)	19, 917, 1414, 1632, 1832, 2204
Bse3DI	GCAATG	1 cut(s)	1389
Bse8I	GATNNNNATC	1 cut(s)	1848
BseBI	CCWGG	3 cut(s)	103, 811, 964
BseCI	ATCGAT	1 cut(s)	1730
BseDI	CCNNGG	4 cut(s)	30, 883, 962, 2192
BseGI	GGATG	7 cut(s)	207, 253, 747, 958, 1062, 1503, 2084
BseJI	GATNNNNATC	1 cut(s)	1848
BseLI	CCNNNNNNNGG	5 cut(s)	102, 127, 183, 1430, 2279
BseMI	GCAATG	1 cut(s)	1389
BseMII	CTCAG	7 cut(s)	58, 113, 155, 717, 1394, 1756, 2307
BseNI	ACTGG	6 cut(s)	19, 917, 1414, 1632, 1832, 2204
BseRI	GAGGAG	2 cut(s)	1536, 1539
BseXI	GCAGC	5 cut(s)	51, 85, 605, 1660, 1980
BsgI	GTGCAG	3 cut(s)	421, 650, 2253
Bsh1236I	CGCG	1 cut(s)	32
BshFI	GGCC	4 cut(s)	134, 779, 1434, 1831
BshNI	GGYRCC	4 cut(s)	423, 1065, 1134, 1154
BshVI	ATCGAT	1 cut(s)	1730
BsiHKAI	GWGCWC	2 cut(s)	198, 1261
BsiHKCI	CYCGRG	1 cut(s)	2124
BsiSI	CCGG	6 cut(s)	302, 883, 1183, 1435, 1921, 2255
BslFI	GGGAC	4 cut(s)	650, 1407, 1730, 2134
BslI	CCNNNNNNNGG	5 cut(s)	102, 127, 183, 1430, 2279
BsmAI	GTCTC	9 cut(s)	59, 144, 800, 895, 1217, 1269, 1449, 1745, 2314
BsmBI	CGTCTC	3 cut(s)	59, 1269, 2314
BsmFI	GGGAC	4 cut(s)	650, 1407, 1730, 2134
BsmI	GAATGC	1 cut(s)	2209
BsnI	GGCC	4 cut(s)	134, 779, 1434, 1831
Bso31I	GGTCTC	2 cut(s)	800, 1745
BsoBI	CYCGRG	1 cut(s)	2124
Bsp1286I	GDGCHC	3 cut(s)	198, 1261, 1618
Bsp1407I	TGTACA	1 cut(s)	2295
Bsp143I	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
BspANI	GGCC	4 cut(s)	134, 779, 1434, 1831
BspCNI	CTCAG	7 cut(s)	57, 114, 154, 718, 1395, 1755, 2308
BspDI	ATCGAT	1 cut(s)	1730
BspFNI	CGCG	1 cut(s)	32
BspPI	GGATC	2 cut(s)	93, 1480
BspQI	GCTCTTC	1 cut(s)	1942
BspT107I	GGYRCC	4 cut(s)	423, 1065, 1134, 1154
BspTNI	GGTCTC	2 cut(s)	800, 1745
BsrDI	GCAATG	1 cut(s)	1389
BsrFI	RCCGGY	2 cut(s)	1182, 1434
BsrGI	TGTACA	1 cut(s)	2295
BsrI	ACTGG	6 cut(s)	19, 917, 1414, 1632, 1832, 2204
BssAI	RCCGGY	2 cut(s)	1182, 1434
BssECI	CCNNGG	4 cut(s)	30, 883, 962, 2192
BssMI	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
BssNAI	GTATAC	1 cut(s)	1754
BssT1I	CCWWGG	1 cut(s)	2192
Bst1107I	GTATAC	1 cut(s)	1754
Bst2UI	CCWGG	3 cut(s)	103, 811, 964
Bst6I	CTCTTC	6 cut(s)	366, 798, 1513, 1869, 1893, 1942
BstAUI	TGTACA	1 cut(s)	2295
BstC8I	GCNNGC	3 cut(s)	37, 1184, 1217
BstDSI	CCRYGG	1 cut(s)	30
BstEII	GGTNACC	1 cut(s)	766
BstF5I	GGATG	7 cut(s)	207, 253, 747, 958, 1062, 1503, 2084
BstFNI	CGCG	1 cut(s)	32
BstHHI	GCGC	1 cut(s)	1038
BstKTI	GATC	8 cut(s)	88, 220, 838, 1462, 1488, 1852, 2074, 2239
BstMAI	GTCTC	9 cut(s)	59, 144, 800, 895, 1217, 1269, 1449, 1745, 2314
BstMBI	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
BstMWI	GCNNNNNNNGC	7 cut(s)	45, 173, 202, 233, 691, 994, 1183
BstNI	CCWGG	3 cut(s)	103, 811, 964
BstPI	GGTNACC	1 cut(s)	766
BstSCI	CCNGG	5 cut(s)	101, 809, 882, 962, 2254
BstSFI	CTRYAG	1 cut(s)	261
BstUI	CGCG	1 cut(s)	32
BstV1I	GCAGC	5 cut(s)	51, 85, 605, 1660, 1980
BstV2I	GAAGAC	4 cut(s)	1043, 1890, 1896, 2104
BstX2I	RGATCY	2 cut(s)	85, 2071
BstXI	CCANNNNNNTGG	1 cut(s)	649
BstYI	RGATCY	2 cut(s)	85, 2071
BstZ17I	GTATAC	1 cut(s)	1754
Bsu15I	ATCGAT	1 cut(s)	1730
BsuI	GTATCC	3 cut(s)	103, 1021, 2301
BsuRI	GGCC	4 cut(s)	134, 779, 1434, 1831
BsuTUI	ATCGAT	1 cut(s)	1730
BtgI	CCRYGG	1 cut(s)	30
BtsCI	GGATG	7 cut(s)	207, 253, 747, 958, 1062, 1503, 2084
BtsIMutI	CAGTG	6 cut(s)	149, 389, 723, 1449, 1718, 1839
Cac8I	GCNNGC	3 cut(s)	37, 1184, 1217
CfoI	GCGC	1 cut(s)	1038
Cfr10I	RCCGGY	2 cut(s)	1182, 1434
Cfr13I	GGNCC	4 cut(s)	636, 664, 777, 880
Cfr42I	CCGCGG	1 cut(s)	33
ClaI	ATCGAT	1 cut(s)	1730
CseI	GACGC	1 cut(s)	76
Csp6I	GTAC	9 cut(s)	12, 187, 424, 607, 1169, 1924, 2144, 2197, 2296
CviAII	CATG	6 cut(s)	25, 625, 688, 955, 1463, 1677
CviQI	GTAC	9 cut(s)	12, 187, 424, 607, 1169, 1924, 2144, 2197, 2296
DpnI	GATC	8 cut(s)	87, 219, 837, 1461, 1487, 1851, 2073, 2238
DpnII	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
DraI	TTTAAA	2 cut(s)	873, 1204
DrdI	GACNNNNNNGTC	2 cut(s)	229, 887
DseDI	GACNNNNNNGTC	2 cut(s)	229, 887
EaeI	YGGCCR	1 cut(s)	132
Eam1104I	CTCTTC	6 cut(s)	366, 798, 1513, 1869, 1893, 1942
EarI	CTCTTC	6 cut(s)	366, 798, 1513, 1869, 1893, 1942
EciI	GGCGGA	1 cut(s)	242
Ecl136II	GAGCTC	1 cut(s)	196
Eco130I	CCWWGG	1 cut(s)	2192
Eco24I	GRGCYC	2 cut(s)	198, 1618
Eco31I	GGTCTC	2 cut(s)	800, 1745
Eco32I	GATATC	4 cut(s)	54, 1300, 1389, 1734
Eco47I	GGWCC	3 cut(s)	636, 664, 880
Eco53kI	GAGCTC	1 cut(s)	196
Eco57I	CTGAAG	2 cut(s)	2019, 2123
Eco88I	CYCGRG	1 cut(s)	2124
Eco91I	GGTNACC	1 cut(s)	766
EcoICRI	GAGCTC	1 cut(s)	196
EcoO109I	RGGNCCY	1 cut(s)	636
EcoO65I	GGTNACC	1 cut(s)	766
EcoRII	CCWGG	3 cut(s)	101, 809, 962
EcoRV	GATATC	4 cut(s)	54, 1300, 1389, 1734
EcoT14I	CCWWGG	1 cut(s)	2192
EcoT22I	ATGCAT	1 cut(s)	960
EcoT38I	GRGCYC	2 cut(s)	198, 1618
ErhI	CCWWGG	1 cut(s)	2192
Esp3I	CGTCTC	3 cut(s)	59, 1269, 2314
FaeI	CATG	6 cut(s)	28, 628, 691, 958, 1466, 1680
FalI	AAGNNNNNCTT	4 cut(s)	495, 527, 2100, 2132
FaqI	GGGAC	4 cut(s)	650, 1407, 1730, 2134
FatI	CATG	6 cut(s)	24, 624, 687, 954, 1462, 1676
FauNDI	CATATG	1 cut(s)	844
FbaI	TGATCA	2 cut(s)	835, 1459
FblI	GTMKAC	1 cut(s)	1753
FokI	GGATG	7 cut(s)	214, 240, 754, 945, 1069, 1510, 2091
FriOI	GRGCYC	2 cut(s)	198, 1618
FspBI	CTAG	4 cut(s)	1212, 1916, 2247, 2312
GlaI	GCGC	1 cut(s)	1037
HaeIII	GGCC	4 cut(s)	134, 779, 1434, 1831
HapII	CCGG	6 cut(s)	302, 883, 1183, 1435, 1921, 2255
HgaI	GACGC	1 cut(s)	76
HhaI	GCGC	1 cut(s)	1038
Hin1II	CATG	6 cut(s)	28, 628, 691, 958, 1466, 1680
Hin6I	GCGC	1 cut(s)	1036
HinP1I	GCGC	1 cut(s)	1036
HindIII	AAGCTT	3 cut(s)	815, 1082, 1128
HpaII	CCGG	6 cut(s)	302, 883, 1183, 1435, 1921, 2255
HphI	GGTGA	8 cut(s)	688, 761, 778, 1072, 1706, 1883, 2141, 2169
Hpy166II	GTNNAC	4 cut(s)	326, 910, 1754, 1926
Hpy188III	TCNNGA	6 cut(s)	121, 199, 1457, 1574, 1763, 2126
Hpy8I	GTNNAC	4 cut(s)	326, 910, 1754, 1926
HpyCH4IV	ACGT	2 cut(s)	830, 1118
HpyF10VI	GCNNNNNNNGC	7 cut(s)	45, 173, 202, 233, 691, 994, 1183
HpySE526I	ACGT	2 cut(s)	830, 1118
Hsp92II	CATG	6 cut(s)	28, 628, 691, 958, 1466, 1680
HspAI	GCGC	1 cut(s)	1036
KpnI	GGTACC	1 cut(s)	427
KroI	GCCGGC	1 cut(s)	1182
KroNI	GCCGGC	1 cut(s)	1184
Ksp22I	TGATCA	2 cut(s)	835, 1459
KspI	CCGCGG	1 cut(s)	33
Kzo9I	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
LguI	GCTCTTC	1 cut(s)	1942
LmnI	GCTCC	3 cut(s)	26, 559, 1199
Lsp1109I	GCAGC	5 cut(s)	51, 85, 605, 1660, 1980
LweI	GCATC	6 cut(s)	192, 929, 967, 1756, 1996, 2231
MaeI	CTAG	4 cut(s)	1212, 1916, 2247, 2312
MaeII	ACGT	2 cut(s)	830, 1118
MaeIII	GTNAC	6 cut(s)	707, 766, 1228, 1363, 1834, 2175
MalI	GATC	8 cut(s)	87, 219, 837, 1461, 1487, 1851, 2073, 2238
MboI	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
MflI	RGATCY	2 cut(s)	85, 2071
MhlI	GDGCHC	3 cut(s)	198, 1261, 1618
MluCI	AATT	9 cut(s)	332, 466, 576, 875, 1681, 1700, 1966, 1988, 2214
MlyI	GAGTC	5 cut(s)	146, 239, 1173, 1768, 2116
Mph1103I	ATGCAT	1 cut(s)	960
MreI	CGCCGGCG	1 cut(s)	1182
MroNI	GCCGGC	1 cut(s)	1182
MroXI	GAANNNNTTC	1 cut(s)	2050
MseI	TTAA	6 cut(s)	872, 1079, 1203, 1782, 1910, 1964
MslI	CAYNNNNRTG	2 cut(s)	321, 1242
MspA1I	CMGCKG	6 cut(s)	32, 98, 782, 1541, 1657, 1673
MspI	CCGG	6 cut(s)	302, 883, 1183, 1435, 1921, 2255
MspR9I	CCNGG	5 cut(s)	103, 811, 884, 964, 2256
Mva1269I	GAATGC	1 cut(s)	2209
MvaI	CCWGG	3 cut(s)	103, 811, 964
MvnI	CGCG	1 cut(s)	32
MwoI	GCNNNNNNNGC	7 cut(s)	45, 173, 202, 233, 691, 994, 1183
NaeI	GCCGGC	1 cut(s)	1184
NciI	CCSGG	2 cut(s)	884, 2256
NdeI	CATATG	1 cut(s)	844
NdeII	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
NgoMIV	GCCGGC	1 cut(s)	1182
NlaIII	CATG	6 cut(s)	28, 628, 691, 958, 1466, 1680
NmeAIII	GCCGAG	1 cut(s)	160
NmuCI	GTSAC	4 cut(s)	766, 1363, 1834, 2175
NsiI	ATGCAT	1 cut(s)	960
OliI	CACNNNNGTG	1 cut(s)	321
PaeR7I	CTCGAG	1 cut(s)	2124
PciSI	GCTCTTC	1 cut(s)	1942
PctI	GAATGC	1 cut(s)	2209
PdiI	GCCGGC	1 cut(s)	1184
PdmI	GAANNNNTTC	1 cut(s)	2050
PfeI	GAWTC	9 cut(s)	250, 298, 455, 461, 653, 1373, 1399, 1570, 1772
PflFI	GACNNNGTC	1 cut(s)	1361
PleI	GAGTC	5 cut(s)	145, 238, 1172, 1767, 2116
PpsI	GAGTC	5 cut(s)	145, 238, 1172, 1767, 2116
PpuMI	RGGWCCY	1 cut(s)	636
Psp124BI	GAGCTC	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	636
Psp6I	CCWGG	3 cut(s)	101, 809, 962
PspEI	GGTNACC	1 cut(s)	766
PspGI	CCWGG	3 cut(s)	101, 809, 962
PspPI	GGNCC	4 cut(s)	636, 664, 777, 880
PspPPI	RGGWCCY	1 cut(s)	636
PsuI	RGATCY	2 cut(s)	85, 2071
PsyI	GACNNNGTC	1 cut(s)	1361
PvuII	CAGCTG	2 cut(s)	1541, 1673
RsaI	GTAC	9 cut(s)	13, 188, 425, 608, 1170, 1925, 2145, 2198, 2297
RsaNI	GTAC	9 cut(s)	12, 187, 424, 607, 1169, 1924, 2144, 2197, 2296
RseI	CAYNNNNRTG	2 cut(s)	321, 1242
SacI	GAGCTC	1 cut(s)	198
SacII	CCGCGG	1 cut(s)	33
SapI	GCTCTTC	1 cut(s)	1942
SaqAI	TTAA	6 cut(s)	872, 1079, 1203, 1782, 1910, 1964
Sau3AI	GATC	8 cut(s)	85, 217, 835, 1459, 1485, 1849, 2071, 2236
Sau96I	GGNCC	4 cut(s)	636, 664, 777, 880
ScaI	AGTACT	2 cut(s)	13, 2145
SchI	GAGTC	5 cut(s)	146, 239, 1173, 1768, 2116
ScrFI	CCNGG	5 cut(s)	103, 811, 884, 964, 2256
SduI	GDGCHC	3 cut(s)	198, 1261, 1618
SfaNI	GCATC	6 cut(s)	192, 929, 967, 1756, 1996, 2231
SfcI	CTRYAG	1 cut(s)	261
Sfr274I	CTCGAG	1 cut(s)	2124
Sfr303I	CCGCGG	1 cut(s)	33
SgrAI	CRCCGGYG	1 cut(s)	1182
SgrBI	CCGCGG	1 cut(s)	33
SinI	GGWCC	3 cut(s)	636, 664, 880
SlaI	CTCGAG	1 cut(s)	2124
SmiMI	CAYNNNNRTG	2 cut(s)	321, 1242
SmlI	CTYRAG	4 cut(s)	974, 1172, 2124, 2158
SmoI	CTYRAG	4 cut(s)	974, 1172, 2124, 2158
Sse9I	AATT	9 cut(s)	332, 466, 576, 875, 1681, 1700, 1966, 1988, 2214
SspMI	CTAG	4 cut(s)	1212, 1916, 2247, 2312
SstI	GAGCTC	1 cut(s)	198
StyD4I	CCNGG	5 cut(s)	101, 809, 882, 962, 2254
StyI	CCWWGG	1 cut(s)	2192
TaiI	ACGT	2 cut(s)	833, 1121
TaqI	TCGA	5 cut(s)	1573, 1730, 1813, 2036, 2125
TaqII	GACCGA	1 cut(s)	1853
TasI	AATT	9 cut(s)	332, 466, 576, 875, 1681, 1700, 1966, 1988, 2214
TatI	WGTACW	3 cut(s)	11, 2143, 2295
TauI	GCSGC	6 cut(s)	21, 35, 239, 782, 904, 2286
TfiI	GAWTC	9 cut(s)	250, 298, 455, 461, 653, 1373, 1399, 1570, 1772
Tru1I	TTAA	6 cut(s)	872, 1079, 1203, 1782, 1910, 1964
Tru9I	TTAA	6 cut(s)	872, 1079, 1203, 1782, 1910, 1964
TscAI	CASTG	6 cut(s)	149, 396, 723, 1456, 1718, 1839
TseFI	GTSAC	4 cut(s)	766, 1363, 1834, 2175
TseI	GCWGC	5 cut(s)	39, 98, 593, 1673, 1993
Tsp45I	GTSAC	4 cut(s)	766, 1363, 1834, 2175
TspDTI	ATGAA	8 cut(s)	140, 242, 410, 513, 613, 1424, 1713, 1860
TspGWI	ACGGA	2 cut(s)	1098, 1813
TspRI	CASTG	6 cut(s)	149, 396, 723, 1456, 1718, 1839
Tth111I	GACNNNGTC	1 cut(s)	1361
VpaK11BI	GGWCC	3 cut(s)	636, 664, 880
XapI	RAATTY	3 cut(s)	332, 466, 1681
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1654
XhoI	CTCGAG	1 cut(s)	2124
XmiI	GTMKAC	1 cut(s)	1753
XmnI	GAANNNNTTC	1 cut(s)	2050
XspI	CTAG	4 cut(s)	1212, 1916, 2247, 2312
ZrmI	AGTACT	2 cut(s)	13, 2145
Zsp2I	ATGCAT	1 cut(s)	960

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.