MD04G1230900.v1.1

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010012
Physical Location & Seq
Forward (+)
31069520 .. 31071383
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1230900.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGACTGATGAGGAACTCTTTGCAGTGGATGATGAAGAAAAGGTGGCAAATGGTGGTGATAATGATGAATATGATGATGTATTGACCGCTGAAGAAAAAAAGCAGTTGGATTCTGCACTTCGTGTGGGGAACTCAGATGGTGCTTGTGAGGATGAGGACAATGGGGTCTCTGACTGCCATGAAAAAGGTTCAGGAGGCTCGTATGAGAACTGTGAATCGAACGGTGTTGTTAAGGAGAAGAAGGGTTGGTTTGGTTGGAACAAGAAAAATTCAAAGGGAAACGATGATTCCGAAGATCCAAAGCCTTTGAAGAAGTTCTCAAAGTTGGCCCAAGAAGGTGGCAACCAGAAATCACTTGATCATCAAAAACCATCATCTGAATTCCCTAAAGACGACATTGCAGATGGGAAAAAGGGAAAAGATAAAAGCAGTAAGAAGAAAAAGAAGAAAGGGTCGACTGATTCTAAGCATGAGAGTGAGTATAAAAAGGGTTTGAGGCCTGTCTTGTGGTTGACGCCAGATTTCCCGTTGAAAGCAGATGAGCTTCTTCCTTTACTAGACATCTTAGCAAACAAGGTCAAGGCTATAAGGCGACTCCGGGAGCTTTTGACCACTAAATTACCCCCTGGCACCTTTCCTGTTAAGGTTGCTATTCCTATTGTCCCAACAATACGAGTTCTTGTAACATTTACCAAATTTGAGGAGCTTCAGCCGGTGGAGGAGTTTTCAACCCCTCTCTCCAGTCCTGCACATTTTCAGGATGCAAAGTCAAAGGACTCAGAAGGATCCTCGTCATGGATGTCATGGGTGAGAGGAAGTCGTGGTGGGCAATCAAGTGACAGCGAAAGCCACCGGTATAAGGATGAAGTTGACCCTTTCCTCATCCCGTCAGACTATACCTGGGTTGATGCCAATGAGAAAAAACGCCGCATGAAGGCCAAGAAAGCCAAGAGCAAGAAACACAGGAGACATGCAACAGCCAAGCCTGCCGAAGCAGGACATCAGGCAAGCGAGGATGTCGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_5 GPCR_chapero_1

Protein Analysis

343

Amino Acids

38.13

Weight (kDa)

6.31

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 19 - 300 3.6e-62 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010012)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04470
fragaria_vesca FvH4_6g01790 FvH4_6g01790 FvH4_6g01790 FvH4_6g01790
malus_domestica MD04G1230900.v1.1 MD12G1249200.v1.1
prunus_persica Prupe.6G350600_v2.0.a1
pyrus_communis pycom04g20450 pycom12g22890
rosa_chinensis RchiOBHm_Chr3g0449511
rosa_laevigata RLG00000025819
rosa_multiflora Rmu_sc0000430.1_g000016
rosa_roxburghii Rroxscaffold_6G00427470
rosa_rugosa Rorug02G0622200 Rorug02G0622300
rosa_samantha Rh3AG020000 Rh3BG020100 Rh3CG018800 Rh3DG020000
rosa_wichuraiana Rw3G001590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 164
AccB1I GGYRCC 1 cut(s) 629
AccI GTMKAC 1 cut(s) 455
AciI CCGC 2 cut(s) 87, 928
AclWI GGATC 3 cut(s) 290, 780, 793
AcsI RAATTY 3 cut(s) 268, 380, 695
AcuI CTGAAG 2 cut(s) 111, 692
AcyI GRCGYC 1 cut(s) 515
AdeI CACNNNGTG 1 cut(s) 122
AfiI CCNNNNNNNGG 3 cut(s) 795, 859, 934
AgeI ACCGGT 1 cut(s) 852
AgsI TTSAA 4 cut(s) 273, 310, 532, 729
AjnI CCWGG 2 cut(s) 625, 899
AluBI AGCT 3 cut(s) 544, 604, 706
AluI AGCT 3 cut(s) 544, 604, 706
Alw26I GTCTC 2 cut(s) 172, 961
AlwI GGATC 3 cut(s) 290, 780, 793
AoxI GGCC 3 cut(s) 327, 497, 936
ApoI RAATTY 3 cut(s) 268, 380, 695
AsiGI ACCGGT 1 cut(s) 852
Asp700I GAANNNNTTC 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 328
AsuC2I CCSGG 1 cut(s) 599
AsuHPI GGTGA 2 cut(s) 68, 820
BamHI GGATCC 1 cut(s) 785
BanI GGYRCC 1 cut(s) 629
BccI CCATC 3 cut(s) 131, 379, 398
BciT130I CCWGG 2 cut(s) 627, 901
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 1 cut(s) 599
BcoDI GTCTC 2 cut(s) 172, 961
BfaI CTAG 1 cut(s) 557
BisI GCNGC 1 cut(s) 928
BlsI GCNGC 1 cut(s) 929
Bme1390I CCNGG 3 cut(s) 599, 627, 901
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 2 cut(s) 631, 787
BmrFI CCNGG 3 cut(s) 599, 627, 901
BmsI GCATC 2 cut(s) 751, 898
BpmI CTGGAG 1 cut(s) 724
BpuMI CCSGG 1 cut(s) 599
BsaHI GRCGYC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 2 cut(s) 625, 900
BsaWI WCCGGW 1 cut(s) 852
Bsc4I CCNNNNNNNGG 3 cut(s) 795, 859, 934
Bse118I RCCGGY 2 cut(s) 712, 852
Bse1I ACTGG 1 cut(s) 741
Bse3DI GCAATG 1 cut(s) 396
BseBI CCWGG 2 cut(s) 627, 901
BseDI CCNNGG 2 cut(s) 625, 900
BseGI GGATG 7 cut(s) 34, 157, 766, 804, 868, 882, 1021
BseLI CCNNNNNNNGG 3 cut(s) 795, 859, 934
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 147, 792
BseNI ACTGG 1 cut(s) 741
BseRI GAGGAG 2 cut(s) 716, 734
BsgI GTGCAG 2 cut(s) 99, 732
BshFI GGCC 3 cut(s) 329, 499, 938
BshNI GGYRCC 1 cut(s) 629
BshTI ACCGGT 1 cut(s) 852
BsiSI CCGG 3 cut(s) 598, 713, 853
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 3 cut(s) 795, 859, 934
BsmAI GTCTC 2 cut(s) 172, 961
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 3 cut(s) 329, 499, 938
Bso31I GGTCTC 1 cut(s) 172
Bsp143I GATC 3 cut(s) 295, 358, 785
BspACI CCGC 2 cut(s) 87, 928
BspANI GGCC 3 cut(s) 329, 499, 938
BspCNI CTCAG 2 cut(s) 146, 791
BspLI GGNNCC 2 cut(s) 631, 787
BspPI GGATC 3 cut(s) 290, 780, 793
BspT107I GGYRCC 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 396
BsrFI RCCGGY 2 cut(s) 712, 852
BsrI ACTGG 1 cut(s) 741
BssAI RCCGGY 2 cut(s) 712, 852
BssECI CCNNGG 2 cut(s) 625, 900
BssMI GATC 3 cut(s) 295, 358, 785
BssNI GRCGYC 1 cut(s) 515
Bst2UI CCWGG 2 cut(s) 627, 901
Bst4CI ACNGT 2 cut(s) 212, 224
BstACI GRCGYC 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 987, 1009
BstDEI CTNAG 4 cut(s) 133, 465, 565, 778
BstF5I GGATG 7 cut(s) 34, 157, 766, 804, 868, 882, 1021
BstKTI GATC 3 cut(s) 298, 361, 788
BstMAI GTCTC 2 cut(s) 172, 961
BstMBI GATC 3 cut(s) 295, 358, 785
BstMWI GCNNNNNNNGC 2 cut(s) 944, 986
BstNI CCWGG 2 cut(s) 627, 901
BstNSI RCATGY 1 cut(s) 974
BstSCI CCNGG 3 cut(s) 597, 625, 899
BstX2I RGATCY 2 cut(s) 295, 785
BstXI CCANNNNNNTGG 1 cut(s) 338
BstYI RGATCY 2 cut(s) 295, 785
BsuRI GGCC 3 cut(s) 329, 499, 938
BtsCI GGATG 7 cut(s) 34, 157, 766, 804, 868, 882, 1021
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 30
Cac8I GCNNGC 2 cut(s) 987, 1009
Cfr10I RCCGGY 2 cut(s) 712, 852
Cfr13I GGNCC 1 cut(s) 328
CseI GACGC 1 cut(s) 523
CspAI ACCGGT 1 cut(s) 852
CviAII CATG 6 cut(s) 179, 470, 795, 804, 931, 971
DdeI CTNAG 4 cut(s) 133, 465, 565, 778
DpnI GATC 3 cut(s) 297, 360, 787
DpnII GATC 3 cut(s) 295, 358, 785
DraIII CACNNNGTG 1 cut(s) 122
DrdI GACNNNNNNGTC 1 cut(s) 164
DseDI GACNNNNNNGTC 1 cut(s) 164
Eco147I AGGCCT 1 cut(s) 499
Eco31I GGTCTC 1 cut(s) 172
Eco57I CTGAAG 2 cut(s) 111, 692
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 2 cut(s) 625, 899
FaeI CATG 6 cut(s) 182, 473, 798, 807, 934, 974
FaqI GGGAC 1 cut(s) 647
FatI CATG 6 cut(s) 178, 469, 794, 803, 930, 970
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 1 cut(s) 928
FokI GGATG 6 cut(s) 41, 164, 773, 811, 869, 875
Fsp4HI GCNGC 1 cut(s) 928
FspBI CTAG 1 cut(s) 557
GluI GCNGC 1 cut(s) 928
GsuI CTGGAG 1 cut(s) 724
HaeIII GGCC 3 cut(s) 329, 499, 938
HapII CCGG 3 cut(s) 598, 713, 853
HgaI GACGC 1 cut(s) 523
Hin1I GRCGYC 1 cut(s) 515
Hin1II CATG 6 cut(s) 182, 473, 798, 807, 934, 974
HincII GTYRAC 3 cut(s) 456, 513, 871
HindII GTYRAC 3 cut(s) 456, 513, 871
HinfI GANTC 6 cut(s) 110, 215, 287, 461, 594, 776
HpaII CCGG 3 cut(s) 598, 713, 853
HphI GGTGA 2 cut(s) 68, 820
Hpy166II GTNNAC 3 cut(s) 456, 513, 871
Hpy188I TCNGA 6 cut(s) 136, 172, 292, 379, 781, 892
Hpy188III TCNNGA 2 cut(s) 192, 758
Hpy8I GTNNAC 3 cut(s) 456, 513, 871
HpyAV CCTTC 4 cut(s) 235, 329, 776, 928
HpyCH4III ACNGT 2 cut(s) 212, 224
HpyCH4V TGCA 6 cut(s) 23, 116, 401, 749, 764, 974
HpyF10VI GCNNNNNNNGC 2 cut(s) 944, 986
HpyF3I CTNAG 4 cut(s) 133, 465, 565, 778
Hsp92I GRCGYC 1 cut(s) 515
Hsp92II CATG 6 cut(s) 182, 473, 798, 807, 934, 974
Ksp22I TGATCA 1 cut(s) 358
Kzo9I GATC 3 cut(s) 295, 358, 785
LmnI GCTCC 2 cut(s) 601, 703
LweI GCATC 2 cut(s) 751, 898
MaeI CTAG 1 cut(s) 557
MaeIII GTNAC 2 cut(s) 682, 836
MalI GATC 3 cut(s) 297, 360, 787
MboI GATC 3 cut(s) 295, 358, 785
MboII GAAGA 8 cut(s) 47, 104, 250, 305, 322, 448, 457, 539
MflI RGATCY 2 cut(s) 295, 785
MluCI AATT 4 cut(s) 268, 380, 617, 695
MlyI GAGTC 2 cut(s) 588, 770
MmeI TCCRAC 2 cut(s) 87, 236
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 2 cut(s) 231, 642
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 3 cut(s) 598, 713, 853
MspR9I CCNGG 3 cut(s) 599, 627, 901
MvaI CCWGG 2 cut(s) 627, 901
MwoI GCNNNNNNNGC 2 cut(s) 944, 986
NciI CCSGG 1 cut(s) 599
NdeII GATC 3 cut(s) 295, 358, 785
NlaIII CATG 6 cut(s) 182, 473, 798, 807, 934, 974
NlaIV GGNNCC 2 cut(s) 631, 787
NmuCI GTSAC 1 cut(s) 836
NspI RCATGY 1 cut(s) 974
PceI AGGCCT 1 cut(s) 499
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 4 cut(s) 110, 215, 287, 461
PfoI TCCNGGA 1 cut(s) 597
PinAI ACCGGT 1 cut(s) 852
PkrI GCNGC 1 cut(s) 929
PleI GAGTC 2 cut(s) 588, 770
PpsI GAGTC 2 cut(s) 588, 770
Psp6I CCWGG 2 cut(s) 625, 899
PspGI CCWGG 2 cut(s) 625, 899
PspN4I GGNNCC 2 cut(s) 631, 787
PspPI GGNCC 1 cut(s) 328
PsuI RGATCY 2 cut(s) 295, 785
RseI CAYNNNNRTG 1 cut(s) 474
SalI GTCGAC 1 cut(s) 454
SaqAI TTAA 2 cut(s) 231, 642
SatI GCNGC 1 cut(s) 928
Sau3AI GATC 3 cut(s) 295, 358, 785
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 2 cut(s) 588, 770
ScrFI CCNGG 3 cut(s) 599, 627, 901
SfaNI GCATC 2 cut(s) 751, 898
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 4 cut(s) 268, 380, 617, 695
SseBI AGGCCT 1 cut(s) 499
SsiI CCGC 2 cut(s) 87, 928
SspMI CTAG 1 cut(s) 557
StuI AGGCCT 1 cut(s) 499
StyD4I CCNGG 3 cut(s) 597, 625, 899
TaaI ACNGT 2 cut(s) 212, 224
TaqI TCGA 3 cut(s) 218, 455, 1020
TasI AATT 4 cut(s) 268, 380, 617, 695
TauI GCSGC 1 cut(s) 930
TfiI GAWTC 4 cut(s) 110, 215, 287, 461
Tru1I TTAA 2 cut(s) 231, 642
Tru9I TTAA 2 cut(s) 231, 642
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 836
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 5 cut(s) 48, 81, 195, 879, 947
TspRI CASTG 1 cut(s) 30
XapI RAATTY 3 cut(s) 268, 380, 695
XceI RCATGY 1 cut(s) 974
XmiI GTMKAC 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.