pycom04g20450

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0010012
Physical Location & Seq
Forward (+)
21959410 .. 21961853
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20450.2

Sequence Viewer

Length: 2034 bp
ATGAAGAGTGAAAGCAGCAATAGCTTAGGAACTGAGAGAACAGTTAAATCCGTAGAATCAGTGGAAGATTTTTCCAAGTATTCCCACAGTCCAGCTCATTTGGCAGTTGCACGCCGTGACTATGCCACTCTCAAGCGCATTATCTCTACCCTCCCTCGACTTGCCAAGGCCAGTGAAGTAAGCACCGAAGATGAATCTCTTGAGGCTGAGCGCCGAGCTGATGCTGTATCTGCTGTCATTGATCGCAGAGACGTTCCTGGTCGTGAGACTCCTCTTCATCTAGCTGTGCGTCTCCGGGACCCAAGCTCAGCGGAGATTTTGATGGCAGCCGGTGCTGATTGGAGTCTTCAAAATGAGAATGGTTGGAGTGCTCTCCAAGAAGCTGTCTGCACTAGAGAGGAAGCAATTGCTATGATCATTGCACGGCACTACCAGCCCCTTGCTTGGGCTAAATGGTGTCGTAGACTTCCCCGTATTGTTGCCTCTACAGCCCGGATTCGTGATTTTTATATGGAGATAAGTTTTCATTTTGAGAGCTCAGTCATCCCGTTTATTGGTCGAATTGCTCCATCAGATACTTACCGAATTTGGAAGCGTGGTTCTAATCTTCGAGCTGACATGACTCTTGCTGGTTTTGATGGGTTTCGCATTCAAAGGTCTGATCAAACATTTCTGTTTCTTGGAGAGGGCTACTCTTCAGAGGACGGTAATGTGAATTTGGCACCTGGTTCTTTGATTGTTCTTGCTCATAAGGAGAAAGAAATCTCAAATGCTTTGGAAGGAGCTGGTGCCCAACCAACCGAAGCTGAAGTTGCCCATGAAGTGGCCTTGATGTCCCAGACGAATATGTTTAGGCCGGGCGTTGATGTTACTCAGGCCGAGCTTATCCCCCATTTAAATTGGAGGCGACAGGAGAGGACTGAGATGGTTGGAAATTGGAAGGCCAAAGTTTATGATATGCTTCACGTGATGGTGAGTGTGAAGTCAAGGCGGGTTCCAGGTGCTATGACTGATGAGGAACTCTTTGCAGTGGATGATGAAGAAAAGGTGGCAAATGGTGGTGATAATGATGAATACGATGATGTATTGACTGCTGAAGAAAAAAAGCAGTTGGATTCTGCACTTCGTGTGGGGAACTCAGATGGTGCTTGTGAGGACGAGGACAATGGGGTATCTGACTGCCATGAAAAAGGTTCAGGAGGCTCGTATGAGAACTGTGAATCGAACGGTGTTGTTAAGGAGAAGAAGGGTTGGTTTGGTTGGAACAAGAAAAATTCAAGGGGAAACGATGATTCTGAAGATCCAAAGCCTTTGAAGAAGTTCTCAAAGTTGGCCCCAGATGGTGGCAACCAGAAATCACTTGATCATCAAAAACCATCATCTGAATTTCCTAAAGACGACATTGCAGATGGGAAAAAGGGAAAAGATAAAAGCAGTAAGAAGAAAAAGAAGAAAGGGTCTACTGATTCTAAGCATGAGAGTGAGTACAAAAAGGGTTTGAGGCCTGTCTTGTGGTTGACGCCAGATTTCCCGTTGAAAACAGATGAGCTTCTTCCTTTACTAGACATCTTAGCAAACAAGGTCAAGGCTATAAGGAGACTCCGGGAGCTTTTGACCACTAAATTACCCCCTGGCACCTTTCCTGTTAAGGTTGCTATTCCTATTGTCCCAACAATACGAGTTCTTATAACATTTACCAAATTTGAGGAGCTTCAGCTGGTGGAGGAGTTTTCAACCCCTCTCTCCAGTCCTGCACATTTTCAGGATGCAAAGTCAAAGGACTCAGAAGGATCCTCGTCATGGATGTCATGGGTGAGAGGAAGTCGTGGTGGGCAATCAAGTGACAGCGAAAGCCACCGGTATAAGGATGAAGTTGACCCTTTCCTCATACCGTCAGACTATACCTGGGTTGATGCCAATGAGAAAAAACGCCGCATGAAGGCCAAGAAAGCCAAGAGCAAGAAACACAGGAGACATGCAACAGCCAAGCCTGCCGAAGCAGGACATCGGGCAAGCGAGGATGTCGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_5 GPCR_chapero_1

Protein Analysis

678

Amino Acids

75.7

Weight (kDa)

6.67

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010012)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04470
fragaria_vesca FvH4_6g01790 FvH4_6g01790 FvH4_6g01790 FvH4_6g01790
malus_domestica MD04G1230900.v1.1 MD12G1249200.v1.1
prunus_persica Prupe.6G350600_v2.0.a1
pyrus_communis pycom04g20450 pycom12g22890
rosa_chinensis RchiOBHm_Chr3g0449511
rosa_laevigata RLG00000025819
rosa_multiflora Rmu_sc0000430.1_g000016
rosa_roxburghii Rroxscaffold_6G00427470
rosa_rugosa Rorug02G0622200 Rorug02G0622300
rosa_samantha Rh3AG020000 Rh3BG020100 Rh3CG018800 Rh3DG020000
rosa_wichuraiana Rw3G001590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1688
AccB1I GGYRCC 3 cut(s) 721, 788, 1634
AccB7I CCANNNNNTGG 2 cut(s) 823, 1343
AccI GTMKAC 2 cut(s) 463, 1460
AciI CCGC 3 cut(s) 311, 991, 1933
AclWI GGATC 3 cut(s) 1295, 1785, 1798
AcsI RAATTY 5 cut(s) 585, 715, 1273, 1385, 1700
AcuI CTGAAG 5 cut(s) 681, 828, 1116, 1317, 1697
AcvI CACGTG 1 cut(s) 967
AcyI GRCGYC 1 cut(s) 1520
AdeI CACNNNGTG 2 cut(s) 116, 1127
AfaI GTAC 1 cut(s) 1487
AfiI CCNNNNNNNGG 8 cut(s) 444, 445, 554, 823, 1343, 1800, 1864, 1939
AgeI ACCGGT 1 cut(s) 1857
AgsI TTSAA 6 cut(s) 350, 653, 1278, 1315, 1537, 1734
AjnI CCWGG 5 cut(s) 256, 724, 997, 1630, 1904
AloI GAACNNNNNNTCC 2 cut(s) 583, 615
Alw21I GWGCWC 2 cut(s) 373, 539
Alw26I GTCTC 5 cut(s) 243, 260, 296, 1591, 1966
AlwI GGATC 3 cut(s) 1295, 1785, 1798
AoxI GGCC 8 cut(s) 168, 825, 854, 876, 942, 1332, 1502, 1941
ApeKI GCWGC 2 cut(s) 15, 326
ApoI RAATTY 5 cut(s) 585, 715, 1273, 1385, 1700
AsiGI ACCGGT 1 cut(s) 1857
Asp700I GAANNNNTTC 1 cut(s) 1319
AspLEI GCGC 2 cut(s) 138, 213
AspS9I GGNCC 2 cut(s) 298, 1333
AsuC2I CCSGG 4 cut(s) 296, 493, 858, 1604
AsuHPI GGTGA 3 cut(s) 985, 1073, 1825
AvaII GGWCC 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 793
BamHI GGATCC 1 cut(s) 1790
BanI GGYRCC 3 cut(s) 721, 788, 1634
BanII GRGCYC 1 cut(s) 539
BbrPI CACGTG 1 cut(s) 967
BbsI GAAGAC 1 cut(s) 338
Bbv12I GWGCWC 2 cut(s) 373, 539
BbvI GCAGC 2 cut(s) 27, 338
BccI CCATC 9 cut(s) 316, 577, 632, 919, 964, 1136, 1334, 1384, 1403
BceAI ACGGC 2 cut(s) 99, 440
BciT130I CCWGG 5 cut(s) 258, 726, 999, 1632, 1906
BclI TGATCA 3 cut(s) 414, 661, 1363
BcnI CCSGG 4 cut(s) 296, 493, 858, 1604
BcoDI GTCTC 5 cut(s) 243, 260, 296, 1591, 1966
BfaI CTAG 3 cut(s) 281, 393, 1562
BfmI CTRYAG 1 cut(s) 486
BfoI RGCGCY 1 cut(s) 214
BisI GCNGC 3 cut(s) 16, 327, 1933
BlpI GCTNAGC 2 cut(s) 207, 307
BlsI GCNGC 3 cut(s) 17, 328, 1934
Bme1390I CCNGG 9 cut(s) 258, 296, 493, 726, 858, 999, 1604, 1632, 1906
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 2 cut(s) 298, 1333
BmiI GGNNCC 8 cut(s) 299, 300, 723, 790, 996, 1335, 1636, 1792
BmrFI CCNGG 9 cut(s) 258, 296, 493, 726, 858, 999, 1604, 1632, 1906
BmsI GCATC 3 cut(s) 211, 1756, 1903
BpiI GAAGAC 1 cut(s) 338
BplI GAGNNNNNCTC 2 cut(s) 677, 709
BpmI CTGGAG 1 cut(s) 1729
Bpu10I CCTNAGC 1 cut(s) 25
Bpu1102I GCTNAGC 2 cut(s) 207, 307
BpuEI CTTGAG 2 cut(s) 116, 221
BpuMI CCSGG 4 cut(s) 296, 493, 858, 1604
BsaAI YACGTR 1 cut(s) 967
BsaHI GRCGYC 1 cut(s) 1520
BsaJI CCNNGG 3 cut(s) 165, 1630, 1905
BsaWI WCCGGW 1 cut(s) 1857
Bsc4I CCNNNNNNNGG 8 cut(s) 444, 445, 554, 823, 1343, 1800, 1864, 1939
Bse118I RCCGGY 2 cut(s) 329, 1857
Bse1I ACTGG 2 cut(s) 171, 1746
Bse3DI GCAATG 2 cut(s) 417, 1401
BseBI CCWGG 5 cut(s) 258, 726, 999, 1632, 1906
BseDI CCNNGG 3 cut(s) 165, 1630, 1905
BseGI GGATG 6 cut(s) 543, 1039, 1771, 1809, 1873, 2026
BseLI CCNNNNNNNGG 8 cut(s) 444, 445, 554, 823, 1343, 1800, 1864, 1939
BseMI GCAATG 2 cut(s) 417, 1401
BseMII CTCAG 8 cut(s) 24, 198, 321, 552, 887, 912, 1152, 1797
BseNI ACTGG 2 cut(s) 171, 1746
BseRI GAGGAG 3 cut(s) 261, 1721, 1739
BseSI GKGCMC 1 cut(s) 793
BseXI GCAGC 2 cut(s) 27, 338
BsgI GTGCAG 3 cut(s) 373, 1104, 1737
BshFI GGCC 8 cut(s) 170, 827, 856, 878, 944, 1334, 1504, 1943
BshNI GGYRCC 3 cut(s) 721, 788, 1634
BshTI ACCGGT 1 cut(s) 1857
BsiHKAI GWGCWC 2 cut(s) 373, 539
BsiSI CCGG 6 cut(s) 295, 330, 493, 857, 1603, 1858
BslFI GGGAC 3 cut(s) 311, 820, 1652
BslI CCNNNNNNNGG 8 cut(s) 444, 445, 554, 823, 1343, 1800, 1864, 1939
BsmAI GTCTC 5 cut(s) 243, 260, 296, 1591, 1966
BsmBI CGTCTC 2 cut(s) 243, 296
BsmFI GGGAC 3 cut(s) 311, 820, 1652
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 8 cut(s) 170, 827, 856, 878, 944, 1334, 1504, 1943
Bsp1286I GDGCHC 3 cut(s) 373, 539, 793
Bsp143I GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
Bsp1720I GCTNAGC 2 cut(s) 207, 307
BspACI CCGC 3 cut(s) 311, 991, 1933
BspANI GGCC 8 cut(s) 170, 827, 856, 878, 944, 1334, 1504, 1943
BspCNI CTCAG 8 cut(s) 25, 199, 320, 551, 886, 913, 1151, 1796
BspLI GGNNCC 8 cut(s) 299, 300, 723, 790, 996, 1335, 1636, 1792
BspPI GGATC 3 cut(s) 1295, 1785, 1798
BspT107I GGYRCC 3 cut(s) 721, 788, 1634
BsrDI GCAATG 2 cut(s) 417, 1401
BsrFI RCCGGY 2 cut(s) 329, 1857
BsrI ACTGG 2 cut(s) 171, 1746
BssAI RCCGGY 2 cut(s) 329, 1857
BssECI CCNNGG 3 cut(s) 165, 1630, 1905
BssMI GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
BssNI GRCGYC 1 cut(s) 1520
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 5 cut(s) 258, 726, 999, 1632, 1906
Bst4CI ACNGT 6 cut(s) 43, 89, 707, 1217, 1229, 1893
Bst6I CTCTTC 2 cut(s) 279, 700
BstACI GRCGYC 1 cut(s) 1520
BstAPI GCANNNNNTGC 1 cut(s) 332
BstBAI YACGTR 1 cut(s) 967
BstC8I GCNNGC 3 cut(s) 112, 1992, 2014
BstF5I GGATG 6 cut(s) 543, 1039, 1771, 1809, 1873, 2026
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 2 cut(s) 138, 213
BstKTI GATC 6 cut(s) 244, 417, 664, 1303, 1366, 1793
BstMAI GTCTC 5 cut(s) 243, 260, 296, 1591, 1966
BstMBI GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
BstMWI GCNNNNNNNGC 9 cut(s) 21, 101, 230, 332, 433, 488, 812, 1949, 1991
BstNI CCWGG 5 cut(s) 258, 726, 999, 1632, 1906
BstNSI RCATGY 1 cut(s) 1979
BstSCI CCNGG 9 cut(s) 256, 294, 491, 724, 856, 997, 1602, 1630, 1904
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 793
BstV1I GCAGC 2 cut(s) 27, 338
BstV2I GAAGAC 1 cut(s) 338
BstX2I RGATCY 2 cut(s) 1300, 1790
BstYI RGATCY 2 cut(s) 1300, 1790
BsuRI GGCC 8 cut(s) 170, 827, 856, 878, 944, 1334, 1504, 1943
BtsCI GGATG 6 cut(s) 543, 1039, 1771, 1809, 1873, 2026
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 3 cut(s) 66, 178, 1035
Cac8I GCNNGC 3 cut(s) 112, 1992, 2014
CfoI GCGC 2 cut(s) 138, 213
Cfr10I RCCGGY 2 cut(s) 329, 1857
Cfr13I GGNCC 2 cut(s) 298, 1333
CseI GACGC 2 cut(s) 278, 1528
CsiI ACCWGGT 1 cut(s) 724
Csp6I GTAC 1 cut(s) 1486
CspAI ACCGGT 1 cut(s) 1857
CviAII CATG 8 cut(s) 619, 818, 1184, 1475, 1800, 1809, 1936, 1976
CviQI GTAC 1 cut(s) 1486
DpnI GATC 6 cut(s) 243, 416, 663, 1302, 1365, 1792
DpnII GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
DraI TTTAAA 1 cut(s) 897
DraIII CACNNNGTG 2 cut(s) 116, 1127
Eam1104I CTCTTC 2 cut(s) 279, 700
EarI CTCTTC 2 cut(s) 279, 700
Ecl136II GAGCTC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 1504
Eco24I GRGCYC 1 cut(s) 539
Eco47I GGWCC 1 cut(s) 298
Eco53kI GAGCTC 1 cut(s) 537
Eco57I CTGAAG 5 cut(s) 681, 828, 1116, 1317, 1697
Eco72I CACGTG 1 cut(s) 967
EcoICRI GAGCTC 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 5 cut(s) 256, 724, 997, 1630, 1904
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 2 cut(s) 243, 296
FaeI CATG 8 cut(s) 622, 821, 1187, 1478, 1803, 1812, 1939, 1979
FaqI GGGAC 3 cut(s) 311, 820, 1652
FatI CATG 8 cut(s) 618, 817, 1183, 1474, 1799, 1808, 1935, 1975
FauI CCCGC 1 cut(s) 984
FbaI TGATCA 3 cut(s) 414, 661, 1363
FblI GTMKAC 2 cut(s) 463, 1460
Fnu4HI GCNGC 3 cut(s) 16, 327, 1933
FokI GGATG 5 cut(s) 530, 1046, 1778, 1816, 1880
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 3 cut(s) 16, 327, 1933
FspBI CTAG 3 cut(s) 281, 393, 1562
GlaI GCGC 2 cut(s) 137, 212
GluI GCNGC 3 cut(s) 16, 327, 1933
GsuI CTGGAG 1 cut(s) 1729
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 8 cut(s) 170, 827, 856, 878, 944, 1334, 1504, 1943
HapII CCGG 6 cut(s) 295, 330, 493, 857, 1603, 1858
HgaI GACGC 2 cut(s) 278, 1528
HhaI GCGC 2 cut(s) 138, 213
Hin1I GRCGYC 1 cut(s) 1520
Hin1II CATG 8 cut(s) 622, 821, 1187, 1478, 1803, 1812, 1939, 1979
Hin6I GCGC 2 cut(s) 136, 211
HinP1I GCGC 2 cut(s) 136, 211
HincII GTYRAC 2 cut(s) 1518, 1876
HindII GTYRAC 2 cut(s) 1518, 1876
HpaII CCGG 6 cut(s) 295, 330, 493, 857, 1603, 1858
HphI GGTGA 3 cut(s) 985, 1073, 1825
Hpy166II GTNNAC 4 cut(s) 464, 1461, 1518, 1876
Hpy188I TCNGA 9 cut(s) 574, 661, 700, 1141, 1177, 1297, 1384, 1786, 1897
Hpy188III TCNNGA 5 cut(s) 200, 263, 500, 1197, 1763
Hpy8I GTNNAC 4 cut(s) 464, 1461, 1518, 1876
HpyAV CCTTC 5 cut(s) 773, 934, 1240, 1781, 1933
HpyCH4III ACNGT 6 cut(s) 43, 89, 707, 1217, 1229, 1893
HpyCH4IV ACGT 2 cut(s) 252, 966
HpyCH4V TGCA 9 cut(s) 110, 390, 422, 1028, 1121, 1406, 1754, 1769, 1979
HpyF10VI GCNNNNNNNGC 9 cut(s) 21, 101, 230, 332, 433, 488, 812, 1949, 1991
HpySE526I ACGT 2 cut(s) 252, 966
Hsp92I GRCGYC 1 cut(s) 1520
Hsp92II CATG 8 cut(s) 622, 821, 1187, 1478, 1803, 1812, 1939, 1979
HspAI GCGC 2 cut(s) 136, 211
KflI GGGWCCC 1 cut(s) 298
Ksp22I TGATCA 3 cut(s) 414, 661, 1363
Kzo9I GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
LmnI GCTCC 4 cut(s) 571, 782, 1606, 1708
Lsp1109I GCAGC 2 cut(s) 27, 338
LweI GCATC 3 cut(s) 211, 1756, 1903
MabI ACCWGGT 1 cut(s) 724
MaeI CTAG 3 cut(s) 281, 393, 1562
MaeII ACGT 2 cut(s) 252, 966
MaeIII GTNAC 3 cut(s) 116, 868, 1841
MalI GATC 6 cut(s) 243, 416, 663, 1302, 1365, 1792
MboI GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 2 cut(s) 1300, 1790
MhlI GDGCHC 3 cut(s) 373, 539, 793
MlyI GAGTC 5 cut(s) 262, 352, 616, 1593, 1775
MmeI TCCRAC 4 cut(s) 344, 910, 1092, 1241
MroXI GAANNNNTTC 1 cut(s) 1319
MseI TTAA 4 cut(s) 45, 896, 1236, 1647
MslI CAYNNNNRTG 1 cut(s) 1479
MspA1I CMGCKG 2 cut(s) 311, 1717
MspI CCGG 6 cut(s) 295, 330, 493, 857, 1603, 1858
MspR9I CCNGG 9 cut(s) 258, 296, 493, 726, 858, 999, 1604, 1632, 1906
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 1 cut(s) 648
MvaI CCWGG 5 cut(s) 258, 726, 999, 1632, 1906
MwoI GCNNNNNNNGC 9 cut(s) 21, 101, 230, 332, 433, 488, 812, 1949, 1991
NciI CCSGG 4 cut(s) 296, 493, 858, 1604
NdeII GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
NlaIII CATG 8 cut(s) 622, 821, 1187, 1478, 1803, 1812, 1939, 1979
NlaIV GGNNCC 8 cut(s) 299, 300, 723, 790, 996, 1335, 1636, 1792
NmeAIII GCCGAG 2 cut(s) 239, 904
NmuCI GTSAC 2 cut(s) 116, 1841
NspI RCATGY 1 cut(s) 1979
PceI AGGCCT 1 cut(s) 1504
PctI GAATGC 1 cut(s) 648
PdmI GAANNNNTTC 1 cut(s) 1319
PfeI GAWTC 7 cut(s) 56, 194, 496, 1115, 1220, 1292, 1466
PflMI CCANNNNNTGG 2 cut(s) 823, 1343
PfoI TCCNGGA 2 cut(s) 294, 1602
PinAI ACCGGT 1 cut(s) 1857
PkrI GCNGC 3 cut(s) 17, 328, 1934
PleI GAGTC 5 cut(s) 262, 351, 616, 1593, 1775
PmaCI CACGTG 1 cut(s) 967
PmlI CACGTG 1 cut(s) 967
PpsI GAGTC 5 cut(s) 262, 351, 616, 1593, 1775
Ppu21I YACGTR 1 cut(s) 967
PpuMI RGGWCCY 1 cut(s) 298
PsiI TTATAA 1 cut(s) 1688
Psp124BI GAGCTC 1 cut(s) 539
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 5 cut(s) 256, 724, 997, 1630, 1904
PspCI CACGTG 1 cut(s) 967
PspGI CCWGG 5 cut(s) 256, 724, 997, 1630, 1904
PspN4I GGNNCC 8 cut(s) 299, 300, 723, 790, 996, 1335, 1636, 1792
PspPI GGNCC 2 cut(s) 298, 1333
PspPPI RGGWCCY 1 cut(s) 298
PsuI RGATCY 2 cut(s) 1300, 1790
PvuII CAGCTG 1 cut(s) 1717
RsaI GTAC 1 cut(s) 1487
RsaNI GTAC 1 cut(s) 1486
RseI CAYNNNNRTG 1 cut(s) 1479
SacI GAGCTC 1 cut(s) 539
SaqAI TTAA 4 cut(s) 45, 896, 1236, 1647
SatI GCNGC 3 cut(s) 16, 327, 1933
Sau3AI GATC 6 cut(s) 241, 414, 661, 1300, 1363, 1790
Sau96I GGNCC 2 cut(s) 298, 1333
SchI GAGTC 5 cut(s) 262, 352, 616, 1593, 1775
ScrFI CCNGG 9 cut(s) 258, 296, 493, 726, 858, 999, 1604, 1632, 1906
SduI GDGCHC 3 cut(s) 373, 539, 793
SexAI ACCWGGT 1 cut(s) 724
SfaNI GCATC 3 cut(s) 211, 1756, 1903
SfcI CTRYAG 1 cut(s) 486
SinI GGWCC 1 cut(s) 298
SmiI ATTTAAAT 1 cut(s) 897
SmiMI CAYNNNNRTG 1 cut(s) 1479
SmlI CTYRAG 2 cut(s) 131, 200
SmoI CTYRAG 2 cut(s) 131, 200
SseBI AGGCCT 1 cut(s) 1504
SsiI CCGC 3 cut(s) 311, 991, 1933
SspMI CTAG 3 cut(s) 281, 393, 1562
SstI GAGCTC 1 cut(s) 539
StuI AGGCCT 1 cut(s) 1504
StyD4I CCNGG 9 cut(s) 256, 294, 491, 724, 856, 997, 1602, 1630, 1904
StyI CCWWGG 1 cut(s) 165
SwaI ATTTAAAT 1 cut(s) 897
TaaI ACNGT 6 cut(s) 43, 89, 707, 1217, 1229, 1893
TaiI ACGT 2 cut(s) 255, 969
TaqI TCGA 5 cut(s) 157, 559, 610, 1223, 2025
TatI WGTACW 1 cut(s) 1485
TauI GCSGC 1 cut(s) 1935
TfiI GAWTC 7 cut(s) 56, 194, 496, 1115, 1220, 1292, 1466
Tru1I TTAA 4 cut(s) 45, 896, 1236, 1647
Tru9I TTAA 4 cut(s) 45, 896, 1236, 1647
TscAI CASTG 3 cut(s) 66, 178, 1035
TseFI GTSAC 2 cut(s) 116, 1841
TseI GCWGC 2 cut(s) 15, 326
Tsp45I GTSAC 2 cut(s) 116, 1841
TspGWI ACGGA 1 cut(s) 40
TspRI CASTG 3 cut(s) 66, 178, 1035
Van91I CCANNNNNTGG 2 cut(s) 823, 1343
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 5 cut(s) 585, 715, 1273, 1385, 1700
XceI RCATGY 1 cut(s) 1979
XmiI GTMKAC 2 cut(s) 463, 1460
XmnI GAANNNNTTC 1 cut(s) 1319
XspI CTAG 3 cut(s) 281, 393, 1562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.