MD12G1249200.v1.1

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010012
Physical Location & Seq
Forward (+)
31872364 .. 31874850
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1249200.v1.1.491

Sequence Viewer

Length: 1650 bp
ATGGCGGCTGGTGCTGATTGGAGTCTTCAAAATGAGAATGGTTGGAGTGCTCTCCAAGAAGCTGTCTGCACTAGAGAGGAAGCAATTGCTATGATTATTGCACGGCACTACCAGCCCCTTGCTTGGGCCAAATGGTGTCGTAGACTTCCCCGTATTGTTGCCTCTACGTCCCGGATTCGTGATTTTTATATGGAGATAACTTTTCATTTTGAGAGCTCAGTCATCCCGTTTATTGGTCGAATTGCTCCATCAGATACTTACCGAATTTGGAAGTCTGATCAAACATTTCTGTTTCTTGGAGAGGGGTACTCTTCAGAGGATGGTAATGTGAATTTGGCACCCGGTTCTTTGATTGTTCTTGCACATAAGGAGAAAGAAATCACAAATGCTTTGGAAGGAGCTGGTGCCCAACCAACTGAAGCTGAAGTTGCCCATGAAGTGGCCTTAATGTCACAGACGAATATGTTTAGGCCCGGCATTGATGTTACTCAGGCTGAGCTTATCCCCCATTTGAATTGGAGGCGACAGGAGAGGACTGAGATGGTTGGAAATTGGAAAGCCAAAGTTTATGATATGCTTCACGTGATGGTGAGTGTGAAGTCAAGGAGGGTTCCAGGTGCTATGACTGACGAGGAACTCTTTGCAGTGGGCGACGAAGAAAAGGTGGCAAATGGTGGTGATAATGATGAATATGACGATGTATTGACTGCTGAAGAAAAAAGGCAGTTGGATTCTGCATTTCTTGTGGGGAACTCAGATGGCGCTTGTGAGGACGAGGACAATGGAGACTCTGACTGTCATGAAAAGGGTTCAGTAGGCTTGTATGAGAACTTTGAATCCAACGGTGTTGTTAAGGACAAGAAGGGTTGGTTTGGTTGGAACAAGAAAAATTCAAAGGGAAACGATGATTCCGAAGATCCAAAGCTTTTGAAGAAGTTCTCGAAGTTGGCCCCAGAAGGTGGGAACCAGAAGTCACTTGATCATCAAAAACCGTCATCTGAATTTCCTAAAGACGATATTGCAGATGCGAAAAAGGGAAAAGATAAAAGCAGTAAGAAGAAAAAGAAGAAAGGGTCGACTGATTCTAAGCATGAGAGTGAGTACAAAAAGGGTTTGAGACCTGTCTTGTGGTTGACGCCAGATTTCCCGTTAAAAACAGATGAGCTTCTTCCTTTACTAGACATCTTAGCAAACAAGGTCAAGGCTATAAGGAGACTCAGGGAGCTTTTGACTACTAAATTGCCCCCTGGCACTTTTCCTGTCAAGGTTGCTATTCCGATTGTCCCAACAATACGAGTTCTTGTAACATTTACCAAATTTGAGGAGCTTCAGCCAGCGGAGGAGTTCTCAACCCCTCTCTCCAGTCCTGCACATTTTCAGGATTCAAAGTCAAAGGACTCAGAAGGATCCTCGTCATGGATGTCGTGGGTGAGAGGAAGTCGTGGTGGGCAATCAAGTGACAGTGATAGCCACCGGTATAAGGATGAGATTGACCCTTTCCTCATACCATCGGACTATACCTGGGTTGATGCCAATGAGAAAAAACGCCGCATGAAAGCCAAGAAAGCCAAGAGCAAGAAACACAGGAGACATGCAACAGCCAAGCCCGGTGATGCAGGACATCAGGCAAGCGAGAATGTCGAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_5 GPCR_chapero_1

Protein Analysis

550

Amino Acids

61.58

Weight (kDa)

6.33

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 85 - 507 7.2e-109 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010012)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04470
fragaria_vesca FvH4_6g01790 FvH4_6g01790 FvH4_6g01790 FvH4_6g01790
malus_domestica MD04G1230900.v1.1 MD12G1249200.v1.1
prunus_persica Prupe.6G350600_v2.0.a1
pyrus_communis pycom04g20450 pycom12g22890
rosa_chinensis RchiOBHm_Chr3g0449511
rosa_laevigata RLG00000025819
rosa_multiflora Rmu_sc0000430.1_g000016
rosa_roxburghii Rroxscaffold_6G00427470
rosa_rugosa Rorug02G0622200 Rorug02G0622300
rosa_samantha Rh3AG020000 Rh3BG020100 Rh3CG018800 Rh3DG020000
rosa_wichuraiana Rw3G001590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 337, 404
AccB7I CCANNNNNTGG 2 cut(s) 439, 959
AccI GTMKAC 2 cut(s) 142, 1076
AciI CCGC 3 cut(s) 5, 1337, 1549
AclWI GGATC 3 cut(s) 911, 1401, 1414
AcsI RAATTY 5 cut(s) 264, 331, 889, 1001, 1316
AcuI CTGAAG 5 cut(s) 297, 438, 444, 732, 1313
AcvI CACGTG 1 cut(s) 583
AcyI GRCGYC 1 cut(s) 1136
AfaI GTAC 2 cut(s) 308, 1103
AfiI CCNNNNNNNGG 7 cut(s) 123, 124, 233, 439, 959, 1416, 1480
AgeI ACCGGT 1 cut(s) 1473
AgsI TTSAA 6 cut(s) 29, 514, 836, 894, 931, 1386
AjnI CCWGG 3 cut(s) 613, 1246, 1520
AloI GAACNNNNNNTCC 2 cut(s) 821, 853
AluBI AGCT 9 cut(s) 62, 216, 401, 422, 499, 925, 1165, 1225, 1327
AluI AGCT 9 cut(s) 62, 216, 401, 422, 499, 925, 1165, 1225, 1327
Alw21I GWGCWC 2 cut(s) 52, 218
Alw26I GTCTC 4 cut(s) 780, 1111, 1207, 1582
AlwI GGATC 3 cut(s) 911, 1401, 1414
AoxI GGCC 4 cut(s) 126, 441, 470, 948
ApoI RAATTY 5 cut(s) 264, 331, 889, 1001, 1316
AsiGI ACCGGT 1 cut(s) 1473
Asp700I GAANNNNTTC 1 cut(s) 935
AspLEI GCGC 1 cut(s) 764
AspS9I GGNCC 3 cut(s) 126, 471, 949
AsuC2I CCSGG 4 cut(s) 172, 342, 474, 1608
AsuHPI GGTGA 4 cut(s) 601, 689, 1441, 1622
BaeGI GKGCMC 1 cut(s) 409
BamHI GGATCC 1 cut(s) 1406
BanI GGYRCC 2 cut(s) 337, 404
BanII GRGCYC 1 cut(s) 218
BbrPI CACGTG 1 cut(s) 583
BbsI GAAGAC 1 cut(s) 17
Bbv12I GWGCWC 2 cut(s) 52, 218
BccI CCATC 6 cut(s) 256, 314, 535, 580, 752, 1516
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 3 cut(s) 615, 1248, 1522
BclI TGATCA 2 cut(s) 277, 979
BcnI CCSGG 4 cut(s) 172, 342, 474, 1608
BcoDI GTCTC 4 cut(s) 780, 1111, 1207, 1582
BfaI CTAG 2 cut(s) 72, 1178
BfoI RGCGCY 1 cut(s) 765
BisI GCNGC 2 cut(s) 6, 1549
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 2 cut(s) 7, 1550
Bme1390I CCNGG 7 cut(s) 172, 342, 474, 615, 1248, 1522, 1608
BmgT120I GGNCC 3 cut(s) 126, 471, 949
BmiI GGNNCC 6 cut(s) 339, 406, 612, 951, 965, 1408
BmrFI CCNGG 7 cut(s) 172, 342, 474, 615, 1248, 1522, 1608
BmsI GCATC 3 cut(s) 1015, 1519, 1603
BpiI GAAGAC 1 cut(s) 17
BplI GAGNNNNNCTC 4 cut(s) 293, 325, 1331, 1363
BpmI CTGGAG 1 cut(s) 1345
Bpu1102I GCTNAGC 1 cut(s) 495
BpuMI CCSGG 4 cut(s) 172, 342, 474, 1608
BsaAI YACGTR 1 cut(s) 583
BsaHI GRCGYC 1 cut(s) 1136
BsaI GGTCTC 1 cut(s) 1111
BsaJI CCNNGG 2 cut(s) 1246, 1521
BsaWI WCCGGW 1 cut(s) 1473
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 124, 233, 439, 959, 1416, 1480
Bse118I RCCGGY 1 cut(s) 1473
Bse1I ACTGG 1 cut(s) 1362
BseBI CCWGG 3 cut(s) 615, 1248, 1522
BseDI CCNNGG 2 cut(s) 1246, 1521
BseGI GGATG 4 cut(s) 222, 325, 1425, 1489
BseLI CCNNNNNNNGG 7 cut(s) 123, 124, 233, 439, 959, 1416, 1480
BseMII CTCAG 7 cut(s) 231, 486, 503, 528, 768, 1231, 1413
BseNI ACTGG 1 cut(s) 1362
BseRI GAGGAG 2 cut(s) 1337, 1355
BseSI GKGCMC 1 cut(s) 409
BsgI GTGCAG 2 cut(s) 52, 1353
BshFI GGCC 4 cut(s) 128, 443, 472, 950
BshNI GGYRCC 2 cut(s) 337, 404
BshTI ACCGGT 1 cut(s) 1473
BsiHKAI GWGCWC 2 cut(s) 52, 218
BsiSI CCGG 5 cut(s) 172, 342, 474, 1474, 1608
BslFI GGGAC 2 cut(s) 154, 1268
BslI CCNNNNNNNGG 7 cut(s) 123, 124, 233, 439, 959, 1416, 1480
BsmAI GTCTC 4 cut(s) 780, 1111, 1207, 1582
BsmFI GGGAC 2 cut(s) 154, 1268
BsnI GGCC 4 cut(s) 128, 443, 472, 950
Bso31I GGTCTC 1 cut(s) 1111
Bsp1286I GDGCHC 3 cut(s) 52, 218, 409
Bsp143I GATC 4 cut(s) 277, 916, 979, 1406
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 3 cut(s) 5, 1337, 1549
BspANI GGCC 4 cut(s) 128, 443, 472, 950
BspCNI CTCAG 7 cut(s) 230, 487, 502, 529, 767, 1230, 1412
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 6 cut(s) 339, 406, 612, 951, 965, 1408
BspPI GGATC 3 cut(s) 911, 1401, 1414
BspT107I GGYRCC 2 cut(s) 337, 404
BspTNI GGTCTC 1 cut(s) 1111
BsrFI RCCGGY 1 cut(s) 1473
BsrI ACTGG 1 cut(s) 1362
BssAI RCCGGY 1 cut(s) 1473
BssECI CCNNGG 2 cut(s) 1246, 1521
BssMI GATC 4 cut(s) 277, 916, 979, 1406
BssNI GRCGYC 1 cut(s) 1136
Bst2UI CCWGG 3 cut(s) 615, 1248, 1522
Bst4CI ACNGT 4 cut(s) 797, 845, 993, 1463
Bst6I CTCTTC 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 1136
BstBAI YACGTR 1 cut(s) 583
BstC8I GCNNGC 2 cut(s) 1335, 1630
BstDEI CTNAG 9 cut(s) 217, 489, 495, 537, 754, 1086, 1186, 1217, 1399
BstF5I GGATG 4 cut(s) 222, 325, 1425, 1489
BstH2I RGCGCY 1 cut(s) 765
BstHHI GCGC 1 cut(s) 764
BstKTI GATC 4 cut(s) 280, 919, 982, 1409
BstMAI GTCTC 4 cut(s) 780, 1111, 1207, 1582
BstMBI GATC 4 cut(s) 277, 916, 979, 1406
BstMWI GCNNNNNNNGC 4 cut(s) 11, 112, 428, 1565
BstNI CCWGG 3 cut(s) 615, 1248, 1522
BstNSI RCATGY 1 cut(s) 1595
BstSCI CCNGG 7 cut(s) 170, 340, 472, 613, 1246, 1520, 1606
BstSLI GKGCMC 1 cut(s) 409
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 916, 1406
BstYI RGATCY 2 cut(s) 916, 1406
BsuRI GGCC 4 cut(s) 128, 443, 472, 950
BtsCI GGATG 4 cut(s) 222, 325, 1425, 1489
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 2 cut(s) 651, 1468
Cac8I GCNNGC 2 cut(s) 1335, 1630
CciI TCATGA 1 cut(s) 799
CfoI GCGC 1 cut(s) 764
Cfr10I RCCGGY 1 cut(s) 1473
Cfr13I GGNCC 3 cut(s) 126, 471, 949
CseI GACGC 1 cut(s) 1144
Csp6I GTAC 2 cut(s) 307, 1102
CspAI ACCGGT 1 cut(s) 1473
CviAII CATG 6 cut(s) 434, 800, 1091, 1416, 1552, 1592
CviQI GTAC 2 cut(s) 307, 1102
DdeI CTNAG 9 cut(s) 217, 489, 495, 537, 754, 1086, 1186, 1217, 1399
DpnI GATC 4 cut(s) 279, 918, 981, 1408
DpnII GATC 4 cut(s) 277, 916, 979, 1406
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Ecl136II GAGCTC 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 218
Eco31I GGTCTC 1 cut(s) 1111
Eco53kI GAGCTC 1 cut(s) 216
Eco57I CTGAAG 5 cut(s) 297, 438, 444, 732, 1313
Eco72I CACGTG 1 cut(s) 583
EcoICRI GAGCTC 1 cut(s) 216
EcoRII CCWGG 3 cut(s) 613, 1246, 1520
EcoT38I GRGCYC 1 cut(s) 218
FaeI CATG 6 cut(s) 437, 803, 1094, 1419, 1555, 1595
FaqI GGGAC 2 cut(s) 154, 1268
FatI CATG 6 cut(s) 433, 799, 1090, 1415, 1551, 1591
FbaI TGATCA 2 cut(s) 277, 979
FblI GTMKAC 2 cut(s) 142, 1076
Fnu4HI GCNGC 2 cut(s) 6, 1549
FokI GGATG 4 cut(s) 209, 332, 1432, 1496
FriOI GRGCYC 1 cut(s) 218
Fsp4HI GCNGC 2 cut(s) 6, 1549
FspBI CTAG 2 cut(s) 72, 1178
GlaI GCGC 1 cut(s) 763
GluI GCNGC 2 cut(s) 6, 1549
GsuI CTGGAG 1 cut(s) 1345
HaeII RGCGCY 1 cut(s) 765
HaeIII GGCC 4 cut(s) 128, 443, 472, 950
HapII CCGG 5 cut(s) 172, 342, 474, 1474, 1608
HgaI GACGC 1 cut(s) 1144
HhaI GCGC 1 cut(s) 764
Hin1I GRCGYC 1 cut(s) 1136
Hin1II CATG 6 cut(s) 437, 803, 1094, 1419, 1555, 1595
Hin6I GCGC 1 cut(s) 762
HinP1I GCGC 1 cut(s) 762
HincII GTYRAC 2 cut(s) 1077, 1134
HindII GTYRAC 2 cut(s) 1077, 1134
HindIII AAGCTT 1 cut(s) 923
HpaII CCGG 5 cut(s) 172, 342, 474, 1474, 1608
HphI GGTGA 4 cut(s) 601, 689, 1441, 1622
Hpy166II GTNNAC 3 cut(s) 143, 1077, 1134
Hpy188III TCNNGA 4 cut(s) 179, 800, 940, 1379
Hpy8I GTNNAC 3 cut(s) 143, 1077, 1134
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 4 cut(s) 389, 856, 950, 1397
HpyCH4III ACNGT 4 cut(s) 797, 845, 993, 1463
HpyCH4IV ACGT 2 cut(s) 167, 582
HpyCH4V TGCA 9 cut(s) 69, 101, 362, 644, 737, 1022, 1370, 1595, 1616
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 112, 428, 1565
HpyF3I CTNAG 9 cut(s) 217, 489, 495, 537, 754, 1086, 1186, 1217, 1399
HpySE526I ACGT 2 cut(s) 167, 582
Hsp92I GRCGYC 1 cut(s) 1136
Hsp92II CATG 6 cut(s) 437, 803, 1094, 1419, 1555, 1595
HspAI GCGC 1 cut(s) 762
Ksp22I TGATCA 2 cut(s) 277, 979
Kzo9I GATC 4 cut(s) 277, 916, 979, 1406
LmnI GCTCC 4 cut(s) 250, 398, 1222, 1324
LweI GCATC 3 cut(s) 1015, 1519, 1603
MaeI CTAG 2 cut(s) 72, 1178
MaeII ACGT 2 cut(s) 167, 582
MaeIII GTNAC 5 cut(s) 450, 484, 972, 1303, 1457
MalI GATC 4 cut(s) 279, 918, 981, 1408
MboI GATC 4 cut(s) 277, 916, 979, 1406
MboII GAAGA 9 cut(s) 17, 303, 668, 725, 926, 943, 1069, 1078, 1160
MfeI CAATTG 1 cut(s) 84
MflI RGATCY 2 cut(s) 916, 1406
MhlI GDGCHC 3 cut(s) 52, 218, 409
MlyI GAGTC 4 cut(s) 31, 782, 1209, 1391
MmeI TCCRAC 5 cut(s) 23, 526, 708, 857, 864
MroXI GAANNNNTTC 1 cut(s) 935
MseI TTAA 3 cut(s) 446, 852, 1151
MslI CAYNNNNRTG 1 cut(s) 1095
MspA1I CMGCKG 1 cut(s) 1337
MspI CCGG 5 cut(s) 172, 342, 474, 1474, 1608
MspR9I CCNGG 7 cut(s) 172, 342, 474, 615, 1248, 1522, 1608
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 3 cut(s) 615, 1248, 1522
MwoI GCNNNNNNNGC 4 cut(s) 11, 112, 428, 1565
NciI CCSGG 4 cut(s) 172, 342, 474, 1608
NdeII GATC 4 cut(s) 277, 916, 979, 1406
NlaIII CATG 6 cut(s) 437, 803, 1094, 1419, 1555, 1595
NlaIV GGNNCC 6 cut(s) 339, 406, 612, 951, 965, 1408
NmuCI GTSAC 3 cut(s) 450, 972, 1457
NspI RCATGY 1 cut(s) 1595
PagI TCATGA 1 cut(s) 799
PcsI WCGNNNNNNNCGW 1 cut(s) 909
PdmI GAANNNNTTC 1 cut(s) 935
PfeI GAWTC 6 cut(s) 175, 731, 836, 908, 1082, 1382
PflMI CCANNNNNTGG 2 cut(s) 439, 959
PfoI TCCNGGA 1 cut(s) 170
PinAI ACCGGT 1 cut(s) 1473
PkrI GCNGC 2 cut(s) 7, 1550
PleI GAGTC 4 cut(s) 30, 782, 1209, 1391
PmaCI CACGTG 1 cut(s) 583
PmlI CACGTG 1 cut(s) 583
PpsI GAGTC 4 cut(s) 30, 782, 1209, 1391
Ppu21I YACGTR 1 cut(s) 583
Psp124BI GAGCTC 1 cut(s) 218
Psp6I CCWGG 3 cut(s) 613, 1246, 1520
PspCI CACGTG 1 cut(s) 583
PspGI CCWGG 3 cut(s) 613, 1246, 1520
PspN4I GGNNCC 6 cut(s) 339, 406, 612, 951, 965, 1408
PspPI GGNCC 3 cut(s) 126, 471, 949
PsuI RGATCY 2 cut(s) 916, 1406
RsaI GTAC 2 cut(s) 308, 1103
RsaNI GTAC 2 cut(s) 307, 1102
RseI CAYNNNNRTG 1 cut(s) 1095
SacI GAGCTC 1 cut(s) 218
SalI GTCGAC 1 cut(s) 1075
SaqAI TTAA 3 cut(s) 446, 852, 1151
SatI GCNGC 2 cut(s) 6, 1549
Sau3AI GATC 4 cut(s) 277, 916, 979, 1406
Sau96I GGNCC 3 cut(s) 126, 471, 949
SchI GAGTC 4 cut(s) 31, 782, 1209, 1391
ScrFI CCNGG 7 cut(s) 172, 342, 474, 615, 1248, 1522, 1608
SduI GDGCHC 3 cut(s) 52, 218, 409
SfaNI GCATC 3 cut(s) 1015, 1519, 1603
SmiMI CAYNNNNRTG 1 cut(s) 1095
SsiI CCGC 3 cut(s) 5, 1337, 1549
SspMI CTAG 2 cut(s) 72, 1178
SstI GAGCTC 1 cut(s) 218
StyD4I CCNGG 7 cut(s) 170, 340, 472, 613, 1246, 1520, 1606
TaaI ACNGT 4 cut(s) 797, 845, 993, 1463
TaiI ACGT 2 cut(s) 170, 585
TaqI TCGA 4 cut(s) 238, 941, 1076, 1641
TatI WGTACW 1 cut(s) 1101
TauI GCSGC 2 cut(s) 8, 1551
TfiI GAWTC 6 cut(s) 175, 731, 836, 908, 1082, 1382
Tru1I TTAA 3 cut(s) 446, 852, 1151
Tru9I TTAA 3 cut(s) 446, 852, 1151
TscAI CASTG 2 cut(s) 651, 1468
TseFI GTSAC 3 cut(s) 450, 972, 1457
Tsp45I GTSAC 3 cut(s) 450, 972, 1457
TspDTI ATGAA 5 cut(s) 194, 450, 702, 816, 1568
TspRI CASTG 2 cut(s) 651, 1468
Van91I CCANNNNNTGG 2 cut(s) 439, 959
XapI RAATTY 5 cut(s) 264, 331, 889, 1001, 1316
XceI RCATGY 1 cut(s) 1595
XmiI GTMKAC 2 cut(s) 142, 1076
XmnI GAANNNNTTC 1 cut(s) 935
XspI CTAG 2 cut(s) 72, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.