pycom12g22890

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010012
Physical Location & Seq
Forward (+)
23663891 .. 23665276
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g22890.1

Sequence Viewer

Length: 1386 bp
ATGAAGAGTGAAAGCAGCAATAGCTTAGGAAATGAGAGAACAGTTAAATCTGTAGAATCAGTGGAAGATATTTCCAAATATTCCCACAGTCCAGCTCATTTGGCGGTTGCATGCCGTGACTACGCCACTCTCAAGCGTATTATTTCTACCCTCCCTCGGCTTGCCAAGGCCAGTGAAGTAAGCACCGAAGATGAATCTCTTGAGGCTGAGCTCCGAGCTGATGCTGTATCTGCTGTCATTGATCGCCGAGATGTTCCTGGTCGTGAGACCCCGCTTCATCTAGCTGTACGTCTCCGGGACCCAAGCTCTGCAGAGATTTTGATGGCGGCTGGTGCTGATTGGAGTCTTCAAAATGAGAATGGTTGGAGTGCTCTCCAAGAAGCTGTCTGCACTAGAGAGGAAGCAATTGCTATGATTATTGCACGGCACTACCAGCCCCTTGCGTGGGCCAAATGGTGTCGTAGACTTCCCCGTATTGTTGCCTCTACATCCCGGATTCGTGATTTTTATATGGAGATGACTTTTCATTTTGAGAGCTCAGTCATCCCGTTTATTGGTCGAATTGCTCCATCAGATACTTACCGAATTTGGAAGCGTGGTTCTAATCTCCGAGCTGACATGACCCTTGCTGGTTTTGATGGGTTTCGCATTCAAAGGTCTGATCAAACATTTCTGTTTCTTGGAGAGGGCTACTCTTCAGAGGATGGTAATGTGAATTTGGCACCTGGTTCTTTGATTGTTCTTGCACATAAGGAGAAAGAAATCACAAATGCTTTGGAAGGAGCTGGTGCTCAACCAACCGAAGCTGAAGTTGCCCATGAAGTGGCCTTAATGTCACAAACGAATATGTTTAGGCCAGGCATTGATGTTACTCAGGCTGAGCTTATCCCCCATTTGAATTGGAGGCGACAGGAGAGGACTGAGACGGTTGGAAATTGGAAAGCCAAAGTTTATGATATGCTTCACGTGATGGTGAGTGTGAAGTCAAGGCGGGTTCCAGGTGCTATGACTGACGAGGAACTCTTTGCAGTGGGCGATGAAGAAAAGGTGGCAAATGGTGGTGATAATGATGAATATGACGATGTATTGACTGCTGAAGAAAAAAGGCAGTTGGATTCTGCATTTCGTGTGGGGAACTCAGATGGTGCTTGTGAGGAAGACGACAATGGAGACTCTGACTGTCATGAAAAAGGTTCAGTAGGCTCGTATGAGAACTTTGAATCCAACGGTGTTGTTAAGGAGAAGAAGGGTTGGTTTGGTTGGAACAAGAAAAATTCGAAGGGAAACGATGATTCCGAAGATCCAAAGCTTTTGAAAAAGTTCTCAAAGTTGGCCCCAGAAGGTGGGAACCAGAAATCACTTGATCGATCAAAAACCATCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_5 GPCR_chapero_1

Protein Analysis

462

Amino Acids

51.31

Weight (kDa)

5.26

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 204 - 434 1.9e-62 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010012)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04470
fragaria_vesca FvH4_6g01790 FvH4_6g01790 FvH4_6g01790 FvH4_6g01790
malus_domestica MD04G1230900.v1.1 MD12G1249200.v1.1
prunus_persica Prupe.6G350600_v2.0.a1
pyrus_communis pycom04g20450 pycom12g22890
rosa_chinensis RchiOBHm_Chr3g0449511
rosa_laevigata RLG00000025819
rosa_multiflora Rmu_sc0000430.1_g000016
rosa_roxburghii Rroxscaffold_6G00427470
rosa_rugosa Rorug02G0622200 Rorug02G0622300
rosa_samantha Rh3AG020000 Rh3BG020100 Rh3CG018800 Rh3DG020000
rosa_wichuraiana Rw3G001590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 721
AccB7I CCANNNNNTGG 2 cut(s) 823, 1343
AccI GTMKAC 1 cut(s) 463
AciI CCGC 4 cut(s) 104, 272, 326, 991
AclWI GGATC 1 cut(s) 1295
AcsI RAATTY 3 cut(s) 585, 715, 1273
AcuI CTGAAG 3 cut(s) 681, 828, 1116
AcvI CACGTG 1 cut(s) 967
AfaI GTAC 1 cut(s) 288
AfiI CCNNNNNNNGG 6 cut(s) 156, 444, 445, 554, 823, 1343
AgsI TTSAA 5 cut(s) 350, 653, 898, 1220, 1315
AjnI CCWGG 4 cut(s) 256, 724, 856, 997
AloI GAACNNNNNNTCC 4 cut(s) 583, 615, 1205, 1237
Alw21I GWGCWC 4 cut(s) 213, 373, 539, 793
Alw26I GTCTC 4 cut(s) 260, 296, 917, 1164
AlwI GGATC 1 cut(s) 1295
AoxI GGCC 5 cut(s) 168, 447, 825, 854, 1332
ApeKI GCWGC 1 cut(s) 15
ApoI RAATTY 3 cut(s) 585, 715, 1273
Asp700I GAANNNNTTC 1 cut(s) 1319
AspS9I GGNCC 3 cut(s) 298, 447, 1333
AsuC2I CCSGG 2 cut(s) 296, 493
AsuHPI GGTGA 2 cut(s) 985, 1073
AsuII TTCGAA 1 cut(s) 1277
AvaII GGWCC 1 cut(s) 298
BanI GGYRCC 1 cut(s) 721
BanII GRGCYC 2 cut(s) 213, 539
BbrPI CACGTG 1 cut(s) 967
BbsI GAAGAC 2 cut(s) 338, 1164
Bbv12I GWGCWC 4 cut(s) 213, 373, 539, 793
BbvI GCAGC 1 cut(s) 27
BccI CCATC 7 cut(s) 316, 577, 632, 698, 964, 1136, 1385
BceAI ACGGC 2 cut(s) 99, 440
BciT130I CCWGG 4 cut(s) 258, 726, 858, 999
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 2 cut(s) 296, 493
BcoDI GTCTC 4 cut(s) 260, 296, 917, 1164
BfaI CTAG 2 cut(s) 281, 393
BfmI CTRYAG 2 cut(s) 51, 309
BisI GCNGC 2 cut(s) 16, 327
BlpI GCTNAGC 2 cut(s) 207, 879
BlsI GCNGC 2 cut(s) 17, 328
Bme1390I CCNGG 6 cut(s) 258, 296, 493, 726, 858, 999
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 3 cut(s) 298, 447, 1333
BmiI GGNNCC 6 cut(s) 299, 300, 723, 996, 1335, 1349
BmrFI CCNGG 6 cut(s) 258, 296, 493, 726, 858, 999
BmsI GCATC 1 cut(s) 211
BpiI GAAGAC 2 cut(s) 338, 1164
BplI GAGNNNNNCTC 2 cut(s) 677, 709
Bpu10I CCTNAGC 1 cut(s) 25
Bpu1102I GCTNAGC 2 cut(s) 207, 879
Bpu14I TTCGAA 1 cut(s) 1277
BpuEI CTTGAG 2 cut(s) 116, 221
BpuMI CCSGG 2 cut(s) 296, 493
Bsa29I ATCGAT 1 cut(s) 1366
BsaAI YACGTR 1 cut(s) 967
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 2 cut(s) 155, 165
Bsc4I CCNNNNNNNGG 6 cut(s) 156, 444, 445, 554, 823, 1343
Bse1I ACTGG 1 cut(s) 171
BseBI CCWGG 4 cut(s) 258, 726, 858, 999
BseCI ATCGAT 1 cut(s) 1366
BseDI CCNNGG 2 cut(s) 155, 165
BseGI GGATG 3 cut(s) 488, 543, 709
BseLI CCNNNNNNNGG 6 cut(s) 156, 444, 445, 554, 823, 1343
BseMII CTCAG 6 cut(s) 198, 552, 870, 887, 912, 1152
BseNI ACTGG 1 cut(s) 171
BseXI GCAGC 1 cut(s) 27
BsgI GTGCAG 1 cut(s) 373
BshFI GGCC 5 cut(s) 170, 449, 827, 856, 1334
BshNI GGYRCC 1 cut(s) 721
BshVI ATCGAT 1 cut(s) 1366
BsiHKAI GWGCWC 4 cut(s) 213, 373, 539, 793
BsiSI CCGG 2 cut(s) 295, 493
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 6 cut(s) 156, 444, 445, 554, 823, 1343
BsmAI GTCTC 4 cut(s) 260, 296, 917, 1164
BsmBI CGTCTC 2 cut(s) 296, 917
BsmFI GGGAC 1 cut(s) 311
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 5 cut(s) 170, 449, 827, 856, 1334
Bso31I GGTCTC 1 cut(s) 260
Bsp119I TTCGAA 1 cut(s) 1277
Bsp1286I GDGCHC 4 cut(s) 213, 373, 539, 793
Bsp143I GATC 5 cut(s) 241, 661, 1300, 1363, 1367
Bsp1720I GCTNAGC 2 cut(s) 207, 879
BspACI CCGC 4 cut(s) 104, 272, 326, 991
BspANI GGCC 5 cut(s) 170, 449, 827, 856, 1334
BspCNI CTCAG 6 cut(s) 199, 551, 871, 886, 913, 1151
BspDI ATCGAT 1 cut(s) 1366
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 6 cut(s) 299, 300, 723, 996, 1335, 1349
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 1 cut(s) 1295
BspT104I TTCGAA 1 cut(s) 1277
BspT107I GGYRCC 1 cut(s) 721
BspTNI GGTCTC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 171
BssECI CCNNGG 2 cut(s) 155, 165
BssMI GATC 5 cut(s) 241, 661, 1300, 1363, 1367
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 4 cut(s) 258, 726, 858, 999
Bst4CI ACNGT 5 cut(s) 43, 89, 928, 1181, 1229
Bst6I CTCTTC 1 cut(s) 700
BstBAI YACGTR 1 cut(s) 967
BstBI TTCGAA 1 cut(s) 1277
BstC8I GCNNGC 2 cut(s) 112, 162
BstDEI CTNAG 7 cut(s) 25, 207, 538, 873, 879, 921, 1138
BstF5I GGATG 3 cut(s) 488, 543, 709
BstKTI GATC 5 cut(s) 244, 664, 1303, 1366, 1370
BstMAI GTCTC 4 cut(s) 260, 296, 917, 1164
BstMBI GATC 5 cut(s) 241, 661, 1300, 1363, 1367
BstMWI GCNNNNNNNGC 6 cut(s) 21, 101, 230, 332, 433, 812
BstNI CCWGG 4 cut(s) 258, 726, 858, 999
BstNSI RCATGY 1 cut(s) 114
BstSCI CCNGG 6 cut(s) 256, 294, 491, 724, 856, 997
BstSFI CTRYAG 2 cut(s) 51, 309
BstV1I GCAGC 1 cut(s) 27
BstV2I GAAGAC 2 cut(s) 338, 1164
BstX2I RGATCY 1 cut(s) 1300
BstYI RGATCY 1 cut(s) 1300
Bsu15I ATCGAT 1 cut(s) 1366
BsuRI GGCC 5 cut(s) 170, 449, 827, 856, 1334
BsuTUI ATCGAT 1 cut(s) 1366
BtgZI GCGATG 1 cut(s) 1050
BtsCI GGATG 3 cut(s) 488, 543, 709
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 3 cut(s) 66, 178, 1035
Cac8I GCNNGC 2 cut(s) 112, 162
CciI TCATGA 1 cut(s) 1183
Cfr13I GGNCC 3 cut(s) 298, 447, 1333
ClaI ATCGAT 1 cut(s) 1366
CsiI ACCWGGT 1 cut(s) 724
Csp6I GTAC 1 cut(s) 287
CviAII CATG 4 cut(s) 111, 619, 818, 1184
CviQI GTAC 1 cut(s) 287
DdeI CTNAG 7 cut(s) 25, 207, 538, 873, 879, 921, 1138
DpnI GATC 5 cut(s) 243, 663, 1302, 1365, 1369
DpnII GATC 5 cut(s) 241, 661, 1300, 1363, 1367
Eam1104I CTCTTC 1 cut(s) 700
EarI CTCTTC 1 cut(s) 700
Ecl136II GAGCTC 2 cut(s) 211, 537
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 213, 539
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 1 cut(s) 298
Eco53kI GAGCTC 2 cut(s) 211, 537
Eco57I CTGAAG 3 cut(s) 681, 828, 1116
Eco72I CACGTG 1 cut(s) 967
EcoICRI GAGCTC 2 cut(s) 211, 537
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 4 cut(s) 256, 724, 856, 997
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 2 cut(s) 213, 539
ErhI CCWWGG 1 cut(s) 165
Esp3I CGTCTC 2 cut(s) 296, 917
FaeI CATG 4 cut(s) 114, 622, 821, 1187
FaqI GGGAC 1 cut(s) 311
FatI CATG 4 cut(s) 110, 618, 817, 1183
FauI CCCGC 2 cut(s) 279, 984
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 2 cut(s) 16, 327
FokI GGATG 3 cut(s) 475, 530, 716
FriOI GRGCYC 2 cut(s) 213, 539
Fsp4HI GCNGC 2 cut(s) 16, 327
FspBI CTAG 2 cut(s) 281, 393
GluI GCNGC 2 cut(s) 16, 327
HaeIII GGCC 5 cut(s) 170, 449, 827, 856, 1334
HapII CCGG 2 cut(s) 295, 493
Hin1II CATG 4 cut(s) 114, 622, 821, 1187
HindIII AAGCTT 1 cut(s) 1307
HinfI GANTC 8 cut(s) 56, 194, 343, 496, 1115, 1172, 1220, 1292
HpaII CCGG 2 cut(s) 295, 493
HphI GGTGA 2 cut(s) 985, 1073
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 9 cut(s) 215, 574, 611, 661, 700, 1141, 1177, 1297, 1385
Hpy188III TCNNGA 4 cut(s) 200, 263, 500, 1184
Hpy8I GTNNAC 1 cut(s) 464
HpyAV CCTTC 4 cut(s) 773, 1240, 1273, 1334
HpyCH4III ACNGT 5 cut(s) 43, 89, 928, 1181, 1229
HpyCH4IV ACGT 2 cut(s) 289, 966
HpyCH4V TGCA 7 cut(s) 110, 311, 390, 422, 746, 1028, 1121
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 101, 230, 332, 433, 812
HpyF3I CTNAG 7 cut(s) 25, 207, 538, 873, 879, 921, 1138
HpySE526I ACGT 2 cut(s) 289, 966
Hsp92II CATG 4 cut(s) 114, 622, 821, 1187
KflI GGGWCCC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 5 cut(s) 241, 661, 1300, 1363, 1367
LmnI GCTCC 3 cut(s) 216, 571, 782
Lsp1109I GCAGC 1 cut(s) 27
LweI GCATC 1 cut(s) 211
MabI ACCWGGT 1 cut(s) 724
MaeI CTAG 2 cut(s) 281, 393
MaeII ACGT 2 cut(s) 289, 966
MaeIII GTNAC 3 cut(s) 116, 834, 868
MalI GATC 5 cut(s) 243, 663, 1302, 1365, 1369
MboI GATC 5 cut(s) 241, 661, 1300, 1363, 1367
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 1 cut(s) 1300
MhlI GDGCHC 4 cut(s) 213, 373, 539, 793
MluCI AATT 7 cut(s) 405, 561, 585, 715, 898, 934, 1273
MlyI GAGTC 2 cut(s) 352, 1166
MmeI TCCRAC 5 cut(s) 344, 910, 1092, 1241, 1248
MroXI GAANNNNTTC 1 cut(s) 1319
MseI TTAA 3 cut(s) 45, 830, 1236
MspI CCGG 2 cut(s) 295, 493
MspR9I CCNGG 6 cut(s) 258, 296, 493, 726, 858, 999
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 1 cut(s) 648
MvaI CCWGG 4 cut(s) 258, 726, 858, 999
MwoI GCNNNNNNNGC 6 cut(s) 21, 101, 230, 332, 433, 812
NciI CCSGG 2 cut(s) 296, 493
NdeII GATC 5 cut(s) 241, 661, 1300, 1363, 1367
NlaIII CATG 4 cut(s) 114, 622, 821, 1187
NlaIV GGNNCC 6 cut(s) 299, 300, 723, 996, 1335, 1349
NmeAIII GCCGAG 2 cut(s) 136, 272
NmuCI GTSAC 2 cut(s) 116, 834
NspI RCATGY 1 cut(s) 114
NspV TTCGAA 1 cut(s) 1277
PaeI GCATGC 1 cut(s) 114
PagI TCATGA 1 cut(s) 1183
PcsI WCGNNNNNNNCGW 1 cut(s) 1293
PctI GAATGC 1 cut(s) 648
PdmI GAANNNNTTC 1 cut(s) 1319
PfeI GAWTC 6 cut(s) 56, 194, 496, 1115, 1220, 1292
PflMI CCANNNNNTGG 2 cut(s) 823, 1343
PfoI TCCNGGA 2 cut(s) 294, 491
PkrI GCNGC 2 cut(s) 17, 328
PleI GAGTC 2 cut(s) 351, 1166
PmaCI CACGTG 1 cut(s) 967
PmlI CACGTG 1 cut(s) 967
PpsI GAGTC 2 cut(s) 351, 1166
Ppu21I YACGTR 1 cut(s) 967
PpuMI RGGWCCY 1 cut(s) 298
Psp124BI GAGCTC 2 cut(s) 213, 539
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 4 cut(s) 256, 724, 856, 997
PspCI CACGTG 1 cut(s) 967
PspGI CCWGG 4 cut(s) 256, 724, 856, 997
PspN4I GGNNCC 6 cut(s) 299, 300, 723, 996, 1335, 1349
PspPI GGNCC 3 cut(s) 298, 447, 1333
PspPPI RGGWCCY 1 cut(s) 298
PstI CTGCAG 1 cut(s) 313
PsuI RGATCY 1 cut(s) 1300
RsaI GTAC 1 cut(s) 288
RsaNI GTAC 1 cut(s) 287
SacI GAGCTC 2 cut(s) 213, 539
SaqAI TTAA 3 cut(s) 45, 830, 1236
SatI GCNGC 2 cut(s) 16, 327
Sau3AI GATC 5 cut(s) 241, 661, 1300, 1363, 1367
Sau96I GGNCC 3 cut(s) 298, 447, 1333
SchI GAGTC 2 cut(s) 352, 1166
ScrFI CCNGG 6 cut(s) 258, 296, 493, 726, 858, 999
SduI GDGCHC 4 cut(s) 213, 373, 539, 793
SexAI ACCWGGT 1 cut(s) 724
SfaNI GCATC 1 cut(s) 211
SfcI CTRYAG 2 cut(s) 51, 309
SfuI TTCGAA 1 cut(s) 1277
SinI GGWCC 1 cut(s) 298
SmlI CTYRAG 2 cut(s) 131, 200
SmoI CTYRAG 2 cut(s) 131, 200
SphI GCATGC 1 cut(s) 114
Sse9I AATT 7 cut(s) 405, 561, 585, 715, 898, 934, 1273
SsiI CCGC 4 cut(s) 104, 272, 326, 991
SspI AATATT 1 cut(s) 80
SspMI CTAG 2 cut(s) 281, 393
SstI GAGCTC 2 cut(s) 213, 539
StyD4I CCNGG 6 cut(s) 256, 294, 491, 724, 856, 997
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 5 cut(s) 43, 89, 928, 1181, 1229
TaiI ACGT 2 cut(s) 292, 969
TaqI TCGA 3 cut(s) 559, 1277, 1366
TasI AATT 7 cut(s) 405, 561, 585, 715, 898, 934, 1273
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 6 cut(s) 56, 194, 496, 1115, 1220, 1292
Tru1I TTAA 3 cut(s) 45, 830, 1236
Tru9I TTAA 3 cut(s) 45, 830, 1236
TscAI CASTG 3 cut(s) 66, 178, 1035
TseFI GTSAC 2 cut(s) 116, 834
TseI GCWGC 1 cut(s) 15
Tsp45I GTSAC 2 cut(s) 116, 834
TspDTI ATGAA 8 cut(s) 17, 207, 266, 515, 834, 1053, 1086, 1200
TspRI CASTG 3 cut(s) 66, 178, 1035
Van91I CCANNNNNTGG 2 cut(s) 823, 1343
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 3 cut(s) 585, 715, 1273
XceI RCATGY 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 463
XmnI GAANNNNTTC 1 cut(s) 1319
XspI CTAG 2 cut(s) 281, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.