Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0016700

Physical Location & Seq

Genomic Coordinates Forward (+)

1107417 .. 1108711

Gene Structure 1295 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1003300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 705 bp

ATGACAGAGAGAAATAGCAATTCTGGTAAAGAAATTGTGTTGAAAGTATTAATGCATTGTGAAGGATGCCAAAGTAAGGTTTCCAAGTGTTTGAGAGGTTTTGAAGGTGTTGAAGAGGTTGTGGTGGATTACCCAAATCATAAAGTGACCGTAAAGGGGAAGAAAGCCGACCCTCTAAGGGTTTTGGAGAGGGTACAAAACAAGTTTAGTAGAAACGCTGAACTCATATCTCCGATACCAAAACCAAAAAACAAACAGAAAAAGGAGCCTGAAAAGAAAGAAGCGGCTCCGCCTCAGGTGAAAGTTGTGGTTCTCAAAACGTTAATGCATTGCGAAGACTGTGCAATTGACGTTAAGAAATACTTGGAGAAAATAAAAGGTGTTGGAAGTGTAGAAGCAAATATGGAGAGCTCAAGGGTGAGAGTGAGGGGAATAGTTGAAGCAGCAAAGCTGGTGGAGTACATAAAGAAGGAATTAGGGAAGCATGCTGAGATAGTGAAGCAAGAACAAGGTGAGGAGAAAGGGCAAGGAAAGGACAAGGCAGATAACAAGGGGCAAGGGAAGGACAAGGCCAGTAATAACAAGCAGCGCCCGGAAGGAGGAGGAGAATGCGTATTCCAATATCCACCACAGTATTCAACCCAGCACATTTACCCTTGTCAAACATTTAGTGATGAAAACCCCCTTGCCTGTTCCACAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0008150 GO:0009987 GO:0019725 GO:0030001 GO:0030003 GO:0042592 GO:0043167 GO:0043169 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055076 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

235

Amino Acids

26.16

Weight (kDa)

9.11

Isoelectric Point (pI)

40.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	17 - 64	4.3e-11	Heavy-metal-associated domain
HMA	PF00403	109 - 162	9.4e-11	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0016700)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02960
fragaria_vesca	FvH4_2g00760
malus_domestica	MD05G1003300.v1.1 MD10G1004300.v1.1
prunus_persica	Prupe.1G222400_v2.0.a1 Prupe.8G006700_v2.0.a1
pyrus_communis	pycom05g00300
rosa_chinensis	RchiOBHm_Chr6g0251141
rosa_laevigata	RLG00000015480
rosa_roxburghii	Rroxscaffold_7G00217000
rosa_samantha	Rh6AG009600 Rh6BG002300 Rh6DG006700
rosa_wichuraiana	Rw6G000960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	284, 290
AclI	AACGTT	1 cut(s)	320
AfaI	GTAC	2 cut(s)	195, 461
AfiI	CCNNNNNNNGG	4 cut(s)	76, 156, 178, 599
AgsI	TTSAA	5 cut(s)	43, 104, 113, 440, 639
AluBI	AGCT	2 cut(s)	411, 451
AluI	AGCT	2 cut(s)	411, 451
Alw21I	GWGCWC	1 cut(s)	413
AoxI	GGCC	1 cut(s)	570
ApeKI	GCWGC	2 cut(s)	443, 586
AseI	ATTAAT	1 cut(s)	50
AspLEI	GCGC	1 cut(s)	591
AsuC2I	CCSGG	1 cut(s)	593
AsuHPI	GGTGA	3 cut(s)	310, 430, 524
AxyI	CCTNAGG	1 cut(s)	294
BanII	GRGCYC	1 cut(s)	413
BbsI	GAAGAC	1 cut(s)	342
Bbv12I	GWGCWC	1 cut(s)	413
BbvI	GCAGC	2 cut(s)	455, 598
BcgI	CGANNNNNNTGC	2 cut(s)	323, 357
BcnI	CCSGG	1 cut(s)	593
BfoI	RGCGCY	1 cut(s)	592
BisI	GCNGC	3 cut(s)	285, 444, 587
BlsI	GCNGC	3 cut(s)	286, 445, 588
Bme1390I	CCNGG	1 cut(s)	593
BmiI	GGNNCC	2 cut(s)	267, 288
BmrFI	CCNGG	1 cut(s)	593
BmsI	GCATC	1 cut(s)	56
BpiI	GAAGAC	1 cut(s)	342
BpuEI	CTTGAG	1 cut(s)	397
BpuMI	CCSGG	1 cut(s)	593
BsaXI	ACNNNNNCTCC	2 cut(s)	597, 627
Bsc4I	CCNNNNNNNGG	4 cut(s)	76, 156, 178, 599
Bse1I	ACTGG	1 cut(s)	573
Bse21I	CCTNAGG	1 cut(s)	294
Bse3DI	GCAATG	1 cut(s)	328
BseGI	GGATG	1 cut(s)	71
BseLI	CCNNNNNNNGG	4 cut(s)	76, 156, 178, 599
BseMI	GCAATG	1 cut(s)	328
BseMII	CTCAG	2 cut(s)	308, 480
BseNI	ACTGG	1 cut(s)	573
BseRI	GAGGAG	3 cut(s)	530, 615, 618
BseXI	GCAGC	2 cut(s)	455, 598
BseYI	CCCAGC	1 cut(s)	642
BshFI	GGCC	1 cut(s)	572
BsiHKAI	GWGCWC	1 cut(s)	413
BsiSI	CCGG	1 cut(s)	593
BslI	CCNNNNNNNGG	4 cut(s)	76, 156, 178, 599
BsmI	GAATGC	1 cut(s)	614
BsnI	GGCC	1 cut(s)	572
Bsp1286I	GDGCHC	1 cut(s)	413
BspACI	CCGC	2 cut(s)	284, 290
BspANI	GGCC	1 cut(s)	572
BspCNI	CTCAG	2 cut(s)	307, 481
BspLI	GGNNCC	2 cut(s)	267, 288
BsrDI	GCAATG	1 cut(s)	328
BsrI	ACTGG	1 cut(s)	573
Bst4CI	ACNGT	3 cut(s)	151, 341, 633
Bst6I	CTCTTC	1 cut(s)	108
BstC8I	GCNNGC	1 cut(s)	486
BstDEI	CTNAG	3 cut(s)	176, 294, 489
BstF5I	GGATG	1 cut(s)	71
BstH2I	RGCGCY	1 cut(s)	592
BstHHI	GCGC	1 cut(s)	591
BstNSI	RCATGY	1 cut(s)	488
BstSCI	CCNGG	1 cut(s)	591
BstV1I	GCAGC	2 cut(s)	455, 598
BstV2I	GAAGAC	1 cut(s)	342
Bsu36I	CCTNAGG	1 cut(s)	294
BsuRI	GGCC	1 cut(s)	572
BtsCI	GGATG	1 cut(s)	71
Cac8I	GCNNGC	1 cut(s)	486
CfoI	GCGC	1 cut(s)	591
Csp6I	GTAC	2 cut(s)	194, 460
CspCI	CAANNNNNGTGG	2 cut(s)	435, 470
CviAII	CATG	1 cut(s)	485
CviJI	RGCY	6 cut(s)	167, 268, 287, 411, 451, 572
CviKI_1	RGCY	6 cut(s)	167, 268, 287, 411, 451, 572
CviQI	GTAC	2 cut(s)	194, 460
DdeI	CTNAG	3 cut(s)	176, 294, 489
Eam1104I	CTCTTC	1 cut(s)	108
EarI	CTCTTC	1 cut(s)	108
EciI	GGCGGA	1 cut(s)	279
Ecl136II	GAGCTC	1 cut(s)	411
Eco24I	GRGCYC	1 cut(s)	413
Eco53kI	GAGCTC	1 cut(s)	411
Eco81I	CCTNAGG	1 cut(s)	294
EcoICRI	GAGCTC	1 cut(s)	411
EcoT22I	ATGCAT	2 cut(s)	57, 330
EcoT38I	GRGCYC	1 cut(s)	413
FaeI	CATG	1 cut(s)	488
FaiI	YATR	5 cut(s)	141, 227, 404, 464, 486
FalI	AAGNNNNNCTT	2 cut(s)	347, 379
FatI	CATG	1 cut(s)	484
Fnu4HI	GCNGC	3 cut(s)	285, 444, 587
FokI	GGATG	1 cut(s)	78
FriOI	GRGCYC	1 cut(s)	413
Fsp4HI	GCNGC	3 cut(s)	285, 444, 587
GlaI	GCGC	1 cut(s)	590
GluI	GCNGC	3 cut(s)	285, 444, 587
GsaI	CCCAGC	1 cut(s)	646
HaeII	RGCGCY	1 cut(s)	592
HaeIII	GGCC	1 cut(s)	572
HapII	CCGG	1 cut(s)	593
HhaI	GCGC	1 cut(s)	591
Hin1II	CATG	1 cut(s)	488
Hin6I	GCGC	1 cut(s)	589
HinP1I	GCGC	1 cut(s)	589
HpaII	CCGG	1 cut(s)	593
HphI	GGTGA	3 cut(s)	310, 430, 524
Hpy188I	TCNGA	1 cut(s)	234
HpyAV	CCTTC	5 cut(s)	56, 98, 463, 556, 590
HpyCH4III	ACNGT	3 cut(s)	151, 341, 633
HpyCH4IV	ACGT	2 cut(s)	320, 351
HpyCH4V	TGCA	3 cut(s)	55, 328, 344
HpyF3I	CTNAG	3 cut(s)	176, 294, 489
HpySE526I	ACGT	2 cut(s)	320, 351
Hsp92II	CATG	1 cut(s)	488
HspAI	GCGC	1 cut(s)	589
LmnI	GCTCC	2 cut(s)	265, 292
LpnPI	CCDG	7 cut(s)	9, 281, 282, 437, 586, 606, 656
Lsp1109I	GCAGC	2 cut(s)	455, 598
LweI	GCATC	1 cut(s)	56
MaeII	ACGT	2 cut(s)	320, 351
MaeIII	GTNAC	1 cut(s)	145
MboII	GAAGA	3 cut(s)	125, 172, 347
MfeI	CAATTG	1 cut(s)	345
MhlI	GDGCHC	1 cut(s)	413
MluCI	AATT	4 cut(s)	19, 33, 345, 473
MmeI	TCCRAC	1 cut(s)	364
MnlI	CCTC	9 cut(s)	89, 109, 183, 183, 303, 420, 508, 593, 596
Mph1103I	ATGCAT	2 cut(s)	57, 330
MseI	TTAA	3 cut(s)	50, 323, 354
MspI	CCGG	1 cut(s)	593
MspR9I	CCNGG	1 cut(s)	593
MunI	CAATTG	1 cut(s)	345
Mva1269I	GAATGC	1 cut(s)	614
NciI	CCSGG	1 cut(s)	593
NlaIII	CATG	1 cut(s)	488
NlaIV	GGNNCC	2 cut(s)	267, 288
NmuCI	GTSAC	1 cut(s)	145
NsiI	ATGCAT	2 cut(s)	57, 330
NspI	RCATGY	1 cut(s)	488
PaeI	GCATGC	1 cut(s)	488
PctI	GAATGC	1 cut(s)	614
PkrI	GCNGC	3 cut(s)	286, 445, 588
PshBI	ATTAAT	1 cut(s)	50
Psp124BI	GAGCTC	1 cut(s)	413
Psp1406I	AACGTT	1 cut(s)	320
PspFI	CCCAGC	1 cut(s)	642
PspN4I	GGNNCC	2 cut(s)	267, 288
RsaI	GTAC	2 cut(s)	195, 461
RsaNI	GTAC	2 cut(s)	194, 460
SacI	GAGCTC	1 cut(s)	413
SaqAI	TTAA	3 cut(s)	50, 323, 354
SatI	GCNGC	3 cut(s)	285, 444, 587
ScrFI	CCNGG	1 cut(s)	593
SduI	GDGCHC	1 cut(s)	413
SfaNI	GCATC	1 cut(s)	56
SmlI	CTYRAG	1 cut(s)	412
SmoI	CTYRAG	1 cut(s)	412
SphI	GCATGC	1 cut(s)	488
Sse9I	AATT	4 cut(s)	19, 33, 345, 473
SsiI	CCGC	2 cut(s)	284, 290
SstI	GAGCTC	1 cut(s)	413
StyD4I	CCNGG	1 cut(s)	591
TaaI	ACNGT	3 cut(s)	151, 341, 633
TaiI	ACGT	2 cut(s)	323, 354
TasI	AATT	4 cut(s)	19, 33, 345, 473
TatI	WGTACW	1 cut(s)	459
TauI	GCSGC	1 cut(s)	287
Tru1I	TTAA	3 cut(s)	50, 323, 354
Tru9I	TTAA	3 cut(s)	50, 323, 354
TseFI	GTSAC	1 cut(s)	145
TseI	GCWGC	2 cut(s)	443, 586
Tsp45I	GTSAC	1 cut(s)	145
TspDTI	ATGAA	1 cut(s)	690
VspI	ATTAAT	1 cut(s)	50
XceI	RCATGY	1 cut(s)	488
Zsp2I	ATGCAT	2 cut(s)	57, 330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.