MD05G1042100.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0019560
Physical Location & Seq
Reverse (-)
7064603 .. 7067981
3379 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1042100.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGAGATGGAATCTTGTGTTCCACCAGGCTTCAGATTTCACCCGACAGAAGAGGAACTTGTGGGTTACTACCTGAAAAGAAAGATCAACTCACTACAAATTGATCTAGATGTCATCGTTGACATTGACTTATACAAAATCGAACCATGGGACATACAAGCGAGATGCAAGCTGGGCTACAGTGAAAAGAATGAGTGGTACTTCTTCAGTCACAAAGACAAGAAGTACCCGACTGGAACTCGAACAAATAGAGCCACAGCTGCTGGATTCTGGAAGGCAACAGGAAGAGATAAGGCAGTGCTTTCAAAGAACAACATTATAGGAATGAGGAAGACCCTGGTGTTCTACAAGGGACGAGCACCTAACGGCAACAAAACCGACTGGATCATGCATGAATATCGACTCCAAACTTCTGAACATGCACCCCCTCAGGAAGAAGGATGGGTTGTATGTCGGGCATTCAAGAAGCCAAGTCCTAGTCACAGGCAAGGTTTTGAAGCTTGGAAGCATGCTTACTATGTCAGAGATCATCCTTCATCTTCAAGCTTCTCGAGTGAAATGCCTATGGCTATGCTGATCAACCCCAATCAAGGTTTCCATCAACCTTTTCGTTCAGATCAGCAAGATCATTTCGTTTCGAACAACATTTTCTTGGGTAACCAACTCATGGAACTTCCTCAACTAGAAAGCCCAACCATTTCAACAAGCTTTGTCACCCAAGAAAACATATTGAATAGCAATAATCTCAGCACTGAAGACTATGAGGACGACAGGAGCAACAACAATAACAGCAATCAACAAATAGATTGGAAAAATTTGGACAATTTACTCGAAGTGCAATTGACTGATAGGGCATCATTTTCTCATCCAAGTTTATCGTCGGGACCCCAATATTATGAGCAAGAAACTCGAAGTAACCATCTTCTTGGATGCTTCCAAGACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0005976 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009733 GO:0009735 GO:0009737 GO:0009741 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010087 GO:0010089 GO:0010383 GO:0010410 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0014070 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032870 GO:0033993 GO:0042221 GO:0042802 GO:0042803 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044036 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0045491 GO:0045893 GO:0045935 GO:0046982 GO:0046983 GO:0048518 GO:0048522 GO:0048759 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071554 GO:0071704 GO:0080090 GO:0090059 GO:0097159 GO:0097305 GO:0098542 GO:0140110 GO:1901348 GO:1901363 GO:1901700 GO:1902680 GO:1903338 GO:1903340 GO:1903506 GO:1903508 GO:1905177 GO:2000112 GO:2000652 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

316

Amino Acids

36.59

Weight (kDa)

6.01

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 8 - 135 1.7e-47 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019560)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71930 AT1G71930
malus_domestica MD05G1042100.v1.1 MD10G1048500.v1.1 MD10G1048600.v1.1
prunus_persica Prupe.8G058100_v2.0.a1
pyrus_communis pycom05g03470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 667
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 1 cut(s) 814
AcuI CTGAAG 3 cut(s) 16, 190, 774
AfaI GTAC 2 cut(s) 200, 227
AfiI CCNNNNNNNGG 2 cut(s) 590, 667
AgsI TTSAA 6 cut(s) 306, 463, 497, 543, 702, 733
AjnI CCWGG 2 cut(s) 25, 336
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AluBI AGCT 5 cut(s) 172, 260, 500, 546, 708
AluI AGCT 5 cut(s) 172, 260, 500, 546, 708
Alw21I GWGCWC 1 cut(s) 361
AlwI GGATC 1 cut(s) 392
AlwNI CAGNNNCTG 1 cut(s) 263
Ama87I CYCGRG 1 cut(s) 550
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 814
AspS9I GGNCC 1 cut(s) 884
AsuHPI GGTGA 2 cut(s) 32, 706
AsuII TTCGAA 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 1 cut(s) 884
AxyI CCTNAGG 1 cut(s) 429
BarI GAAGNNNNNNTAC 2 cut(s) 497, 529
BbsI GAAGAC 2 cut(s) 338, 762
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 247
BccI CCATC 3 cut(s) 435, 606, 927
BceAI ACGGC 1 cut(s) 382
BcgI CGANNNNNNTGC 6 cut(s) 380, 414, 541, 575, 890, 924
BciT130I CCWGG 2 cut(s) 27, 338
BclI TGATCA 1 cut(s) 576
BfaI CTAG 3 cut(s) 107, 477, 683
BfmI CTRYAG 1 cut(s) 178
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 27, 338
Bme18I GGWCC 1 cut(s) 884
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 1 cut(s) 884
BmiI GGNNCC 2 cut(s) 885, 886
BmrFI CCNGG 2 cut(s) 27, 338
BmsI GCATC 3 cut(s) 155, 863, 920
BpiI GAAGAC 2 cut(s) 338, 762
Bpu14I TTCGAA 1 cut(s) 638
BsaJI CCNNGG 2 cut(s) 146, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 590, 667
Bse1I ACTGG 2 cut(s) 238, 386
Bse21I CCTNAGG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 27, 338
BseDI CCNNGG 2 cut(s) 146, 336
BseGI GGATG 4 cut(s) 446, 529, 865, 935
BseLI CCNNNNNNNGG 2 cut(s) 590, 667
BseMII CTCAG 2 cut(s) 443, 760
BseNI ACTGG 2 cut(s) 238, 386
BseXI GCAGC 1 cut(s) 247
BseYI CCCAGC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 550
BslFI GGGAC 3 cut(s) 164, 366, 897
BslI CCNNNNNNNGG 2 cut(s) 590, 667
BsmFI GGGAC 3 cut(s) 164, 366, 897
BsmI GAATGC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 550
Bsp119I TTCGAA 1 cut(s) 638
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
Bsp19I CCATGG 1 cut(s) 146
BspCNI CTCAG 2 cut(s) 442, 759
BspLI GGNNCC 2 cut(s) 885, 886
BspPI GGATC 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 638
BsrI ACTGG 2 cut(s) 238, 386
BssECI CCNNGG 2 cut(s) 146, 336
BssMI GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 2 cut(s) 27, 338
Bst4CI ACNGT 1 cut(s) 182
Bst6I CTCTTC 2 cut(s) 45, 280
BstBI TTCGAA 1 cut(s) 638
BstC8I GCNNGC 2 cut(s) 170, 510
BstDEI CTNAG 2 cut(s) 429, 746
BstDSI CCRYGG 1 cut(s) 146
BstEII GGTNACC 1 cut(s) 656
BstF5I GGATG 4 cut(s) 446, 529, 865, 935
BstKTI GATC 7 cut(s) 87, 106, 387, 529, 579, 619, 628
BstMBI GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
BstMWI GCNNNNNNNGC 2 cut(s) 174, 260
BstNI CCWGG 2 cut(s) 27, 338
BstNSI RCATGY 2 cut(s) 422, 512
BstPI GGTNACC 1 cut(s) 656
BstSCI CCNGG 2 cut(s) 25, 336
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 247
BstV2I GAAGAC 2 cut(s) 338, 762
BstXI CCANNNNNNTGG 1 cut(s) 926
Bsu36I CCTNAGG 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 146
BtsCI GGATG 4 cut(s) 446, 529, 865, 935
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 3 cut(s) 187, 303, 750
Cac8I GCNNGC 2 cut(s) 170, 510
CaiI CAGNNNCTG 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 884
Csp6I GTAC 2 cut(s) 199, 226
CviAII CATG 6 cut(s) 147, 388, 392, 419, 509, 667
CviQI GTAC 2 cut(s) 199, 226
DdeI CTNAG 2 cut(s) 429, 746
DpnI GATC 7 cut(s) 86, 105, 386, 528, 578, 618, 627
DpnII GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
Eam1104I CTCTTC 2 cut(s) 45, 280
EarI CTCTTC 2 cut(s) 45, 280
Eco130I CCWWGG 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 884
Eco57I CTGAAG 3 cut(s) 16, 190, 774
Eco81I CCTNAGG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 550
Eco91I GGTNACC 1 cut(s) 656
EcoO109I RGGNCCY 1 cut(s) 884
EcoO65I GGTNACC 1 cut(s) 656
EcoRII CCWGG 2 cut(s) 25, 336
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 6 cut(s) 150, 391, 395, 422, 512, 670
FalI AAGNNNNNCTT 2 cut(s) 42, 74
FaqI GGGAC 3 cut(s) 164, 366, 897
FatI CATG 6 cut(s) 146, 387, 391, 418, 508, 666
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 4 cut(s) 453, 516, 852, 942
Fsp4HI GCNGC 1 cut(s) 261
FspBI CTAG 3 cut(s) 107, 477, 683
GluI GCNGC 1 cut(s) 261
GsaI CCCAGC 1 cut(s) 176
Hin1II CATG 6 cut(s) 150, 391, 395, 422, 512, 670
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 3 cut(s) 498, 544, 706
HinfI GANTC 3 cut(s) 11, 267, 402
HphI GGTGA 2 cut(s) 32, 706
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 4 cut(s) 35, 415, 524, 616
Hpy188III TCNNGA 6 cut(s) 107, 271, 431, 463, 550, 882
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 3 cut(s) 268, 431, 543
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4V TGCA 4 cut(s) 168, 391, 422, 838
HpyF10VI GCNNNNNNNGC 2 cut(s) 174, 260
HpyF3I CTNAG 2 cut(s) 429, 746
Hsp92II CATG 6 cut(s) 150, 391, 395, 422, 512, 670
KflI GGGWCCC 1 cut(s) 884
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
LmnI GCTCC 1 cut(s) 774
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 3 cut(s) 155, 863, 920
MaeI CTAG 3 cut(s) 107, 477, 683
MaeIII GTNAC 6 cut(s) 65, 209, 479, 656, 712, 914
MalI GATC 7 cut(s) 86, 105, 386, 528, 578, 618, 627
MboI GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
MboII GAAGA 8 cut(s) 62, 196, 297, 343, 446, 531, 767, 914
MfeI CAATTG 1 cut(s) 839
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 4 cut(s) 99, 814, 823, 839
MlyI GAGTC 1 cut(s) 396
MnlI CCTC 5 cut(s) 46, 321, 438, 687, 757
Mph1103I ATGCAT 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 260
MspR9I CCNGG 2 cut(s) 27, 338
MunI CAATTG 1 cut(s) 839
Mva1269I GAATGC 1 cut(s) 458
MvaI CCWGG 2 cut(s) 27, 338
MwoI GCNNNNNNNGC 2 cut(s) 174, 260
NcoI CCATGG 1 cut(s) 146
NdeII GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
NlaIII CATG 6 cut(s) 150, 391, 395, 422, 512, 670
NlaIV GGNNCC 2 cut(s) 885, 886
NmuCI GTSAC 3 cut(s) 209, 479, 712
NsiI ATGCAT 1 cut(s) 393
NspI RCATGY 2 cut(s) 422, 512
NspV TTCGAA 1 cut(s) 638
PaeI GCATGC 1 cut(s) 512
PaeR7I CTCGAG 1 cut(s) 550
PctI GAATGC 1 cut(s) 458
PfeI GAWTC 2 cut(s) 11, 267
PflMI CCANNNNNTGG 1 cut(s) 667
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
PpuMI RGGWCCY 1 cut(s) 884
Psp5II RGGWCCY 1 cut(s) 884
Psp6I CCWGG 2 cut(s) 25, 336
PspEI GGTNACC 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 2 cut(s) 25, 336
PspN4I GGNNCC 2 cut(s) 885, 886
PspPI GGNCC 1 cut(s) 884
PspPPI RGGWCCY 1 cut(s) 884
PstNI CAGNNNCTG 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 2 cut(s) 200, 227
RsaNI GTAC 2 cut(s) 199, 226
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 7 cut(s) 84, 103, 384, 526, 576, 616, 625
Sau96I GGNCC 1 cut(s) 884
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 2 cut(s) 27, 338
SduI GDGCHC 1 cut(s) 361
SfaNI GCATC 3 cut(s) 155, 863, 920
SfcI CTRYAG 1 cut(s) 178
Sfr274I CTCGAG 1 cut(s) 550
SfuI TTCGAA 1 cut(s) 638
SinI GGWCC 1 cut(s) 884
SlaI CTCGAG 1 cut(s) 550
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
SphI GCATGC 1 cut(s) 512
Sse9I AATT 4 cut(s) 99, 814, 823, 839
SspI AATATT 1 cut(s) 893
SspMI CTAG 3 cut(s) 107, 477, 683
StyD4I CCNGG 2 cut(s) 25, 336
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 182
TaqI TCGA 7 cut(s) 141, 241, 400, 551, 638, 831, 910
TasI AATT 4 cut(s) 99, 814, 823, 839
TfiI GAWTC 2 cut(s) 11, 267
TscAI CASTG 3 cut(s) 187, 303, 757
TseFI GTSAC 3 cut(s) 209, 479, 712
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 3 cut(s) 209, 479, 712
TspDTI ATGAA 2 cut(s) 408, 525
TspRI CASTG 3 cut(s) 187, 303, 757
Van91I CCANNNNNTGG 1 cut(s) 667
VpaK11BI GGWCC 1 cut(s) 884
XapI RAATTY 1 cut(s) 814
XbaI TCTAGA 1 cut(s) 106
XceI RCATGY 2 cut(s) 422, 512
XhoI CTCGAG 1 cut(s) 550
XspI CTAG 3 cut(s) 107, 477, 683
Zsp2I ATGCAT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.