MD05G1050300.v1.1

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009828
Physical Location & Seq
Reverse (-)
8721451 .. 8726290
4840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1050300.v1.1.491

Sequence Viewer

Length: 1044 bp
ATGCGTAAAAGTCAAAGCAAAAAATTGGGAGGTCCAGACAGTCAGTCAGTCAGTCAGTCTCTCACTTGGAGTAGAGATATAGAGATATCACCATCACGGCTCCTCCTCTTTAACATTCCTCTCTTCCCCCACCGCCCAGATATCACCATCCTTTTGCAGTTAGCGTATATTCTGTCAGCTATGGACAATGGTTCTGGTCCAAGTTTGTTTCCGCTGCACCGATGCAAAACTCTTCACCTGGTGAGGCATGGACAAGGAATCCACAATGTAGAGGGAGCTAAGAACTACAAAGCATACATGAAACCTGAATATTTTGATGCACGCCTTACTCCACTAGGCTGGCAGCAGGTTGATAATTTGCGTAAGCATGTTCATGAATGTGGGCTTTCCAAGAGGATTGAGTTAGTCATTACATCTCCTTTGCTAAGGACTCTGCAAACGGCTGTCGGAGTATTTGGAGGTGAGGGTTACAAGGATAGAACGGACGTACCACCTCTAATGGTGGCAAATGCAGGAAATAGTGAACGCCCATCGATTTCAAATCTCAACTGCCCACCAATCATTGCGGTAGAGCTTTGTCGAGAACATTTGGGCGTTCATCCCTGTGATAACAGGAGGAACATCAGCGACTATCAGTTTCTATTTCCTGCGGTTGATTTTTCGCTGATAGAAACTGATGAGGATATATTGTGGAAGGCCGATGTGAGGGAGACGACAGAGGAAGTTGCAGCTAGGGGATTGAAATTCATGAACTGGTTGTGGACAAGGAAAGAGAAAGAAATAGCGATTGTTACTCATAACGGGTTCTTGTCTCACACGCTAAACGCACTAGGAAATGACTGTCATCCTTCGATGAAAAAAGAAATGTCCAAACACTTTTCAAATTGCGAGCTTCGCTCCATGGTCATTGTTGACAGAAGTATGGCAGGGTCCGATTCATCAACAACTGATTATCCAGGAAAGATTCCATTGGGGCCGGATCTCCCTAGTGATGTTGCTGATGAGAAGTACCGTGGGAAAGAAAAGGTCCGAACTCAAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

348

Amino Acids

39.06

Weight (kDa)

8.11

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 78 - 151 3.4e-06 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 337
AciI CCGC 4 cut(s) 133, 212, 566, 650
AclWI GGATC 1 cut(s) 987
AcsI RAATTY 1 cut(s) 743
AdeI CACNNNGTG 1 cut(s) 241
AfaI GTAC 2 cut(s) 489, 1010
AfiI CCNNNNNNNGG 1 cut(s) 705
AgsI TTSAA 3 cut(s) 540, 742, 882
AhdI GACNNNNNGTC 1 cut(s) 43
AjnI CCWGG 2 cut(s) 237, 955
AluBI AGCT 5 cut(s) 179, 278, 574, 731, 892
AluI AGCT 5 cut(s) 179, 278, 574, 731, 892
Alw26I GTCTC 3 cut(s) 63, 704, 816
AlwI GGATC 1 cut(s) 987
AoxI GGCC 2 cut(s) 696, 974
ApeKI GCWGC 3 cut(s) 214, 343, 728
ApoI RAATTY 1 cut(s) 743
AspS9I GGNCC 5 cut(s) 32, 197, 930, 974, 1027
AsuHPI GGTGA 5 cut(s) 81, 136, 227, 253, 473
AvaII GGWCC 4 cut(s) 32, 197, 930, 1027
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BbvI GCAGC 3 cut(s) 201, 355, 740
BccI CCATC 3 cut(s) 100, 155, 538
BceAI ACGGC 2 cut(s) 113, 456
BciT130I CCWGG 2 cut(s) 239, 957
BcoDI GTCTC 3 cut(s) 63, 704, 816
BfaI CTAG 4 cut(s) 335, 732, 830, 987
BfuAI ACCTGC 1 cut(s) 337
BisI GCNGC 3 cut(s) 215, 344, 729
BlsI GCNGC 3 cut(s) 216, 345, 730
Bme1390I CCNGG 2 cut(s) 239, 957
Bme18I GGWCC 4 cut(s) 32, 197, 930, 1027
BmeRI GACNNNNNGTC 1 cut(s) 43
BmgT120I GGNCC 5 cut(s) 32, 197, 930, 974, 1027
BmiI GGNNCC 3 cut(s) 101, 931, 975
BmrFI CCNGG 2 cut(s) 239, 957
BmsI GCATC 2 cut(s) 212, 307
BplI GAGNNNNNCTC 2 cut(s) 881, 913
Bpu10I CCTNAGC 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 1020
Bsa29I ATCGAT 1 cut(s) 533
BsaJI CCNNGG 2 cut(s) 900, 1012
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bsc4I CCNNNNNNNGG 1 cut(s) 705
Bse1I ACTGG 1 cut(s) 758
Bse3DI GCAATG 1 cut(s) 561
BseBI CCWGG 2 cut(s) 239, 957
BseCI ATCGAT 1 cut(s) 533
BseDI CCNNGG 2 cut(s) 900, 1012
BseGI GGATG 3 cut(s) 147, 598, 844
BseLI CCNNNNNNNGG 1 cut(s) 705
BseMI GCAATG 1 cut(s) 561
BseNI ACTGG 1 cut(s) 758
BseRI GAGGAG 2 cut(s) 92, 95
BseXI GCAGC 3 cut(s) 201, 355, 740
BsgI GTGCAG 1 cut(s) 200
BshFI GGCC 2 cut(s) 698, 976
BshVI ATCGAT 1 cut(s) 533
BsiSI CCGG 1 cut(s) 977
BslI CCNNNNNNNGG 1 cut(s) 705
BsmAI GTCTC 3 cut(s) 63, 704, 816
BsmBI CGTCTC 1 cut(s) 704
BsnI GGCC 2 cut(s) 698, 976
Bsp143I GATC 1 cut(s) 979
Bsp19I CCATGG 1 cut(s) 900
BspACI CCGC 4 cut(s) 133, 212, 566, 650
BspANI GGCC 2 cut(s) 698, 976
BspDI ATCGAT 1 cut(s) 533
BspHI TCATGA 2 cut(s) 373, 747
BspLI GGNNCC 3 cut(s) 101, 931, 975
BspMI ACCTGC 1 cut(s) 337
BspPI GGATC 1 cut(s) 987
BsrDI GCAATG 1 cut(s) 561
BsrI ACTGG 1 cut(s) 758
BssECI CCNNGG 2 cut(s) 900, 1012
BssMI GATC 1 cut(s) 979
BssT1I CCWWGG 1 cut(s) 900
Bst2UI CCWGG 2 cut(s) 239, 957
Bst4CI ACNGT 3 cut(s) 41, 842, 1013
Bst6I CTCTTC 2 cut(s) 128, 237
BstC8I GCNNGC 3 cut(s) 322, 341, 890
BstDEI CTNAG 2 cut(s) 279, 425
BstDSI CCRYGG 2 cut(s) 900, 1012
BstF5I GGATG 3 cut(s) 147, 598, 844
BstKTI GATC 1 cut(s) 982
BstMAI GTCTC 3 cut(s) 63, 704, 816
BstMBI GATC 1 cut(s) 979
BstMWI GCNNNNNNNGC 1 cut(s) 894
BstNI CCWGG 2 cut(s) 239, 957
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 2 cut(s) 237, 955
BstV1I GCAGC 3 cut(s) 201, 355, 740
BstX2I RGATCY 1 cut(s) 979
BstXI CCANNNNNNTGG 1 cut(s) 339
BstYI RGATCY 1 cut(s) 979
Bsu15I ATCGAT 1 cut(s) 533
BsuRI GGCC 2 cut(s) 698, 976
BsuTUI ATCGAT 1 cut(s) 533
BtgI CCRYGG 2 cut(s) 900, 1012
BtsCI GGATG 3 cut(s) 147, 598, 844
BveI ACCTGC 1 cut(s) 337
Cac8I GCNNGC 3 cut(s) 322, 341, 890
CciI TCATGA 2 cut(s) 373, 747
Cfr13I GGNCC 5 cut(s) 32, 197, 930, 974, 1027
ClaI ATCGAT 1 cut(s) 533
CsiI ACCWGGT 1 cut(s) 237
Csp6I GTAC 2 cut(s) 488, 1009
CviAII CATG 6 cut(s) 248, 298, 368, 374, 748, 901
CviQI GTAC 2 cut(s) 488, 1009
DdeI CTNAG 2 cut(s) 279, 425
DpnI GATC 1 cut(s) 981
DpnII GATC 1 cut(s) 979
DraIII CACNNNGTG 1 cut(s) 241
DriI GACNNNNNGTC 1 cut(s) 43
Eam1104I CTCTTC 2 cut(s) 128, 237
Eam1105I GACNNNNNGTC 1 cut(s) 43
EarI CTCTTC 2 cut(s) 128, 237
Eco130I CCWWGG 1 cut(s) 900
Eco32I GATATC 2 cut(s) 87, 142
Eco47I GGWCC 4 cut(s) 32, 197, 930, 1027
EcoRII CCWGG 2 cut(s) 237, 955
EcoRV GATATC 2 cut(s) 87, 142
EcoT14I CCWWGG 1 cut(s) 900
ErhI CCWWGG 1 cut(s) 900
Esp3I CGTCTC 1 cut(s) 704
FaeI CATG 6 cut(s) 251, 301, 371, 377, 751, 904
FatI CATG 6 cut(s) 247, 297, 367, 373, 747, 900
Fnu4HI GCNGC 3 cut(s) 215, 344, 729
FokI GGATG 3 cut(s) 134, 585, 831
Fsp4HI GCNGC 3 cut(s) 215, 344, 729
FspBI CTAG 4 cut(s) 335, 732, 830, 987
GluI GCNGC 3 cut(s) 215, 344, 729
HaeIII GGCC 2 cut(s) 698, 976
HapII CCGG 1 cut(s) 977
Hin1II CATG 6 cut(s) 251, 301, 371, 377, 751, 904
HincII GTYRAC 1 cut(s) 913
HindII GTYRAC 1 cut(s) 913
HinfI GANTC 4 cut(s) 258, 430, 935, 964
HpaII CCGG 1 cut(s) 977
HphI GGTGA 5 cut(s) 81, 136, 227, 253, 473
Hpy166II GTNNAC 3 cut(s) 524, 762, 913
Hpy188I TCNGA 3 cut(s) 449, 934, 1031
Hpy188III TCNNGA 4 cut(s) 35, 374, 581, 748
Hpy8I GTNNAC 3 cut(s) 524, 762, 913
HpyAV CCTTC 2 cut(s) 688, 858
HpyCH4III ACNGT 3 cut(s) 41, 842, 1013
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 7 cut(s) 157, 217, 225, 320, 436, 512, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 894
HpyF3I CTNAG 2 cut(s) 279, 425
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 6 cut(s) 251, 301, 371, 377, 751, 904
Kzo9I GATC 1 cut(s) 979
LmnI GCTCC 3 cut(s) 105, 275, 902
Lsp1109I GCAGC 3 cut(s) 201, 355, 740
LweI GCATC 2 cut(s) 212, 307
MabI ACCWGGT 1 cut(s) 237
MaeI CTAG 4 cut(s) 335, 732, 830, 987
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 467, 790
MalI GATC 1 cut(s) 981
MboI GATC 1 cut(s) 979
MboII GAAGA 2 cut(s) 115, 224
MflI RGATCY 1 cut(s) 979
MluCI AATT 4 cut(s) 23, 355, 743, 883
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 427
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 3 cut(s) 372, 378, 603
MspA1I CMGCKG 1 cut(s) 214
MspI CCGG 1 cut(s) 977
MspR9I CCNGG 2 cut(s) 239, 957
MvaI CCWGG 2 cut(s) 239, 957
MwoI GCNNNNNNNGC 1 cut(s) 894
NcoI CCATGG 1 cut(s) 900
NdeII GATC 1 cut(s) 979
NlaIII CATG 6 cut(s) 251, 301, 371, 377, 751, 904
NlaIV GGNNCC 3 cut(s) 101, 931, 975
NspI RCATGY 1 cut(s) 371
PagI TCATGA 2 cut(s) 373, 747
PfeI GAWTC 3 cut(s) 258, 935, 964
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 3 cut(s) 216, 345, 730
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 2 cut(s) 237, 955
PspGI CCWGG 2 cut(s) 237, 955
PspN4I GGNNCC 3 cut(s) 101, 931, 975
PspPI GGNCC 5 cut(s) 32, 197, 930, 974, 1027
PsuI RGATCY 1 cut(s) 979
RsaI GTAC 2 cut(s) 489, 1010
RsaNI GTAC 2 cut(s) 488, 1009
RseI CAYNNNNRTG 3 cut(s) 372, 378, 603
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 3 cut(s) 215, 344, 729
Sau3AI GATC 1 cut(s) 979
Sau96I GGNCC 5 cut(s) 32, 197, 930, 974, 1027
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 239, 957
SexAI ACCWGGT 1 cut(s) 237
SfaNI GCATC 2 cut(s) 212, 307
SinI GGWCC 4 cut(s) 32, 197, 930, 1027
SmiMI CAYNNNNRTG 3 cut(s) 372, 378, 603
SmlI CTYRAG 1 cut(s) 1035
SmoI CTYRAG 1 cut(s) 1035
Sse9I AATT 4 cut(s) 23, 355, 743, 883
SsiI CCGC 4 cut(s) 133, 212, 566, 650
SspI AATATT 1 cut(s) 311
SspMI CTAG 4 cut(s) 335, 732, 830, 987
StyD4I CCNGG 2 cut(s) 237, 955
StyI CCWWGG 1 cut(s) 900
TaaI ACNGT 3 cut(s) 41, 842, 1013
TaiI ACGT 1 cut(s) 489
TaqI TCGA 3 cut(s) 533, 580, 851
TasI AATT 4 cut(s) 23, 355, 743, 883
TfiI GAWTC 3 cut(s) 258, 935, 964
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TseI GCWGC 3 cut(s) 214, 343, 728
TspDTI ATGAA 8 cut(s) 314, 362, 390, 587, 736, 764, 869, 927
TspGWI ACGGA 1 cut(s) 497
VpaK11BI GGWCC 4 cut(s) 32, 197, 930, 1027
XapI RAATTY 1 cut(s) 743
XceI RCATGY 1 cut(s) 371
XspI CTAG 4 cut(s) 335, 732, 830, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.