MD10G1057600.v1.1

Phosphoglycerate mutase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009828
Physical Location & Seq
Reverse (-)
7817594 .. 7822999
5406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1057600.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGAGAATGGTTCAGGTCCAAGTTTGTTTCCGCTGCACCGATGCAAAACTCTTCACCTGGTGAGGCATGGACAAGGAATCCACAATGTAGAAGGAGCTAAGAACTACAAAGCATACATGAAACCTGAATATTTTGATGCACAACTTACTCCACTAGGCTGGCAGCAGGTTGATAATTTGCGTAAGCATGTTCATGAATGTGGGCTTTCCAAGAGGATTGAGTTGGTCATCACATCTCCTTTGCTAAGGACTCTGCAAACGGCTGTTGGAGTATTTGGAGGCGAGGGTTACAAGGATGGAATGGACGTGCTGCCTCTAATGGTGGAAAATGCAGGAAATAGTGAACGCTCTTCAATTTCAAGTCTCAACTGCCCGCCAATCATTGCAGTAGAGCTTTGTCGAGAACATTTGGGCGTTCATCCGTGTGATAGCAGGAGGAATATCAGCAACTATCAGTTTCTATTTCCTGCTCTTAATTTTTCTCTGATAGAAACTGATGAGGATGTATTGTGGGAGGCCGATGTGAGGGAGACGACAGAGGAAGTTGCAGCTAGGGGAATGAAATTCATGAACTGGTTGTGGACAAGGAAAGAGAAAGAAATAGCGATTGTTACTCATAACGGGTTCTTGTTTCACACGCTAAATGCACTAGGAAATGATTGTCATCCTTCGATGAAAAAAGAAATGTCCACACACTTTGCAAATTGCGAACTTCGCTCCATGATCATTGTTGACAGAAGTATGACAGGGTCCGATTTATCAACAACTGATTATCCAGGAAAGATTCCTCCGGGGCCGGATCTCCCTAGTGATGTTGCTGATGAAAAGAAGCGTGAGAAAGAAAAGGACCGAACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

286

Amino Acids

32.15

Weight (kDa)

6.07

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 18 - 91 2e-06 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 157
AciI CCGC 2 cut(s) 32, 374
AclWI GGATC 1 cut(s) 807
AcsI RAATTY 1 cut(s) 563
AdeI CACNNNGTG 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 525
AgsI TTSAA 2 cut(s) 354, 360
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 2 cut(s) 57, 775
AluBI AGCT 3 cut(s) 98, 394, 551
AluI AGCT 3 cut(s) 98, 394, 551
Alw26I GTCTC 2 cut(s) 368, 524
AlwI GGATC 1 cut(s) 807
AoxI GGCC 2 cut(s) 516, 794
ApeKI GCWGC 4 cut(s) 34, 163, 310, 548
ApoI RAATTY 1 cut(s) 563
AspS9I GGNCC 4 cut(s) 17, 750, 794, 847
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 2 cut(s) 47, 73
AvaII GGWCC 3 cut(s) 17, 750, 847
BbvI GCAGC 4 cut(s) 21, 175, 297, 560
BccI CCATC 1 cut(s) 290
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 2 cut(s) 59, 777
BclI TGATCA 1 cut(s) 723
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 2 cut(s) 368, 524
BfaI CTAG 4 cut(s) 155, 552, 650, 807
BfuAI ACCTGC 1 cut(s) 157
BisI GCNGC 4 cut(s) 35, 164, 311, 549
BlsI GCNGC 4 cut(s) 36, 165, 312, 550
Bme1390I CCNGG 3 cut(s) 59, 777, 792
Bme18I GGWCC 3 cut(s) 17, 750, 847
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 4 cut(s) 17, 750, 794, 847
BmiI GGNNCC 2 cut(s) 751, 795
BmrFI CCNGG 3 cut(s) 59, 777, 792
BmsI GCATC 2 cut(s) 32, 127
Bpu10I CCTNAGC 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 792
BsaBI GATNNNNATC 1 cut(s) 663
BsaJI CCNNGG 1 cut(s) 791
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse1I ACTGG 1 cut(s) 578
Bse3DI GCAATG 1 cut(s) 381
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 2 cut(s) 59, 777
BseDI CCNNGG 1 cut(s) 791
BseGI GGATG 4 cut(s) 301, 418, 508, 664
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMI GCAATG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 578
BseXI GCAGC 4 cut(s) 21, 175, 297, 560
BsgI GTGCAG 1 cut(s) 20
BshFI GGCC 2 cut(s) 518, 796
BsiSI CCGG 2 cut(s) 791, 797
BslI CCNNNNNNNGG 1 cut(s) 525
BsmAI GTCTC 2 cut(s) 368, 524
BsmBI CGTCTC 1 cut(s) 524
BsnI GGCC 2 cut(s) 518, 796
Bsp143I GATC 2 cut(s) 723, 799
BspACI CCGC 2 cut(s) 32, 374
BspANI GGCC 2 cut(s) 518, 796
BspHI TCATGA 2 cut(s) 193, 567
BspLI GGNNCC 2 cut(s) 751, 795
BspMI ACCTGC 1 cut(s) 157
BspPI GGATC 1 cut(s) 807
BspQI GCTCTTC 1 cut(s) 355
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 1 cut(s) 578
BssECI CCNNGG 1 cut(s) 791
BssMI GATC 2 cut(s) 723, 799
Bst2UI CCWGG 2 cut(s) 59, 777
Bst6I CTCTTC 2 cut(s) 57, 355
BstC8I GCNNGC 2 cut(s) 161, 374
BstDEI CTNAG 3 cut(s) 99, 245, 855
BstF5I GGATG 4 cut(s) 301, 418, 508, 664
BstKTI GATC 2 cut(s) 726, 802
BstMAI GTCTC 2 cut(s) 368, 524
BstMBI GATC 2 cut(s) 723, 799
BstMWI GCNNNNNNNGC 1 cut(s) 714
BstNI CCWGG 2 cut(s) 59, 777
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 3 cut(s) 57, 775, 790
BstV1I GCAGC 4 cut(s) 21, 175, 297, 560
BstX2I RGATCY 1 cut(s) 799
BstXI CCANNNNNNTGG 1 cut(s) 159
BstYI RGATCY 1 cut(s) 799
BsuRI GGCC 2 cut(s) 518, 796
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 4 cut(s) 301, 418, 508, 664
BveI ACCTGC 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 161, 374
CciI TCATGA 2 cut(s) 193, 567
Cfr13I GGNCC 4 cut(s) 17, 750, 794, 847
CsiI ACCWGGT 1 cut(s) 57
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CviAII CATG 6 cut(s) 68, 118, 188, 194, 568, 721
CviJI RGCY 8 cut(s) 98, 159, 205, 263, 394, 518, 551, 796
CviKI_1 RGCY 8 cut(s) 98, 159, 205, 263, 394, 518, 551, 796
DdeI CTNAG 3 cut(s) 99, 245, 855
DpnI GATC 2 cut(s) 725, 801
DpnII GATC 2 cut(s) 723, 799
DraIII CACNNNGTG 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 57, 355
EarI CTCTTC 2 cut(s) 57, 355
Eco47I GGWCC 3 cut(s) 17, 750, 847
EcoRII CCWGG 2 cut(s) 57, 775
Esp3I CGTCTC 1 cut(s) 524
FaeI CATG 6 cut(s) 71, 121, 191, 197, 571, 724
FaiI YATR 9 cut(s) 69, 115, 119, 189, 195, 569, 618, 722, 743
FatI CATG 6 cut(s) 67, 117, 187, 193, 567, 720
FauI CCCGC 1 cut(s) 381
FbaI TGATCA 1 cut(s) 723
Fnu4HI GCNGC 4 cut(s) 35, 164, 311, 549
FokI GGATG 4 cut(s) 308, 405, 515, 651
Fsp4HI GCNGC 4 cut(s) 35, 164, 311, 549
FspBI CTAG 4 cut(s) 155, 552, 650, 807
GluI GCNGC 4 cut(s) 35, 164, 311, 549
HaeIII GGCC 2 cut(s) 518, 796
HapII CCGG 2 cut(s) 791, 797
Hin1II CATG 6 cut(s) 71, 121, 191, 197, 571, 724
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 3 cut(s) 78, 250, 784
HpaII CCGG 2 cut(s) 791, 797
HphI GGTGA 2 cut(s) 47, 73
Hpy166II GTNNAC 4 cut(s) 344, 582, 690, 733
Hpy188I TCNGA 2 cut(s) 486, 754
Hpy188III TCNNGA 3 cut(s) 194, 401, 568
Hpy8I GTNNAC 4 cut(s) 344, 582, 690, 733
HpyAV CCTTC 2 cut(s) 86, 678
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 9 cut(s) 37, 45, 140, 256, 332, 386, 548, 647, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 714
HpyF3I CTNAG 3 cut(s) 99, 245, 855
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 6 cut(s) 71, 121, 191, 197, 571, 724
Ksp22I TGATCA 1 cut(s) 723
Kzo9I GATC 2 cut(s) 723, 799
LguI GCTCTTC 1 cut(s) 355
LmnI GCTCC 2 cut(s) 95, 722
Lsp1109I GCAGC 4 cut(s) 21, 175, 297, 560
LweI GCATC 2 cut(s) 32, 127
MabI ACCWGGT 1 cut(s) 57
MaeI CTAG 4 cut(s) 155, 552, 650, 807
MaeII ACGT 1 cut(s) 306
MaeIII GTNAC 2 cut(s) 287, 610
MalI GATC 2 cut(s) 725, 801
MboI GATC 2 cut(s) 723, 799
MboII GAAGA 2 cut(s) 44, 342
MflI RGATCY 1 cut(s) 799
MluCI AATT 5 cut(s) 175, 354, 475, 563, 703
MlyI GAGTC 1 cut(s) 244
MmeI TCCRAC 1 cut(s) 247
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 3 cut(s) 192, 198, 423
MspA1I CMGCKG 1 cut(s) 34
MspI CCGG 2 cut(s) 791, 797
MspR9I CCNGG 3 cut(s) 59, 777, 792
MvaI CCWGG 2 cut(s) 59, 777
MwoI GCNNNNNNNGC 1 cut(s) 714
NciI CCSGG 1 cut(s) 792
NdeII GATC 2 cut(s) 723, 799
NlaIII CATG 6 cut(s) 71, 121, 191, 197, 571, 724
NlaIV GGNNCC 2 cut(s) 751, 795
NspI RCATGY 1 cut(s) 191
PagI TCATGA 2 cut(s) 193, 567
PciSI GCTCTTC 1 cut(s) 355
PfeI GAWTC 2 cut(s) 78, 784
PflFI GACNNNGTC 1 cut(s) 748
PfoI TCCNGGA 1 cut(s) 775
PkrI GCNGC 4 cut(s) 36, 165, 312, 550
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 57, 775
PspGI CCWGG 2 cut(s) 57, 775
PspN4I GGNNCC 2 cut(s) 751, 795
PspPI GGNCC 4 cut(s) 17, 750, 794, 847
PsuI RGATCY 1 cut(s) 799
PsyI GACNNNGTC 1 cut(s) 748
RseI CAYNNNNRTG 3 cut(s) 192, 198, 423
SapI GCTCTTC 1 cut(s) 355
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 4 cut(s) 35, 164, 311, 549
Sau3AI GATC 2 cut(s) 723, 799
Sau96I GGNCC 4 cut(s) 17, 750, 794, 847
SchI GAGTC 1 cut(s) 244
ScrFI CCNGG 3 cut(s) 59, 777, 792
SetI ASST 8 cut(s) 19, 60, 100, 127, 171, 309, 396, 553
SexAI ACCWGGT 1 cut(s) 57
SfaNI GCATC 2 cut(s) 32, 127
SinI GGWCC 3 cut(s) 17, 750, 847
SmiMI CAYNNNNRTG 3 cut(s) 192, 198, 423
Sse9I AATT 5 cut(s) 175, 354, 475, 563, 703
SsiI CCGC 2 cut(s) 32, 374
SspI AATATT 1 cut(s) 131
SspMI CTAG 4 cut(s) 155, 552, 650, 807
StyD4I CCNGG 3 cut(s) 57, 775, 790
TaiI ACGT 1 cut(s) 309
TaqI TCGA 2 cut(s) 400, 671
TasI AATT 5 cut(s) 175, 354, 475, 563, 703
TfiI GAWTC 2 cut(s) 78, 784
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TseI GCWGC 4 cut(s) 34, 163, 310, 548
TspDTI ATGAA 9 cut(s) 134, 182, 210, 407, 556, 575, 584, 689, 837
TspGWI ACGGA 1 cut(s) 411
Tth111I GACNNNGTC 1 cut(s) 748
VpaK11BI GGWCC 3 cut(s) 17, 750, 847
XapI RAATTY 1 cut(s) 563
XceI RCATGY 1 cut(s) 191
XspI CTAG 4 cut(s) 155, 552, 650, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.