Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0016750

Physical Location & Seq

Genomic Coordinates Reverse (-)

15161290 .. 15162036

Gene Structure 747 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1072100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 747 bp

ATGGATATTGGACGTGATGATGTTTGTGGTTTTGAATGCAACATCTGCTTGGCTTTAGCGCAAGACCCTGTTATAACACCCTGTGGCCACCTCTACTGCTGGAGTTGTATGATGAAATGGCTTTTCAAGGCACATTCCAACAATGAGTGCCCAATTTGTAGAACTGGCATAGAATTCAGCCAACTCATCTTTTCTTGCGGAAAATCGAAAATACAATCGAATCCAAAATCTCCGTTTGAATGCAAAATTTGCTCCGAATTAGCCCGAGACCCAATTGTCACACTTTGTGGCCACCTTTATTGTTGGTGTTGTTTGAACAAATGGCTTAACAAATGGAATTCAAATTCCAACTGTTGGGTTTGCAAGGCGGTTATAGAGGATGAAAAATTAGTCCCTCTCTATGGAAAAGGGACCAAATCACATGGTGTCTCACGTCCGGGATCATGGTCTGCAACCGTTGGCACTGCCGGCACTGCCTTAAGTCTCGAAGTGAAATGTTTTGTTCAACGTGTATTTGCTACGATTGGAGCTCTATTTCGGTTTGGTCGACGTACCATGGAAGTTTTTATGACAAGAGTGTTACCAATTATTGCAATTCTTGTGATGTTGCATCCCTATTTGCCAAGGGAGAGAAAGAATGATTTGGTGGAGAAGGTTTTGGTTTGGAGCTTGTGGTCTTGGTTGATTGTAGTCATTTGGGATGTCCTCAATGGGAACATTAAACGACATAAAAAACTGGTACATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0031984 GO:0032446 GO:0032527 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0051788 GO:0070647 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071702 GO:0071704 GO:0071705 GO:0071712 GO:0098796 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

zf-C3HC4 zf-C3HC4_3

Protein Analysis

249

Amino Acids

28.23

Weight (kDa)

8.93

Isoelectric Point (pI)

43.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	12 - 58	3.3e-11	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	12 - 54	1.5e-09	Ring finger domain
zf-C3HC4_2	PF13923	12 - 53	7.5e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	12 - 55	1.4e-07	zinc-RING finger domain
zf-C3HC4	PF00097	13 - 53	1.7e-09	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	13 - 51	3.1e-09	RING-type zinc-finger
zf-C3HC4_4	PF15227	13 - 53	2.5e-07	zinc finger of C3HC4-type, RING
Prok-RING_4	PF14447	13 - 58	1.2e-06	Prokaryotic RING finger family 4
zf-C3HC4_3	PF13920	80 - 126	1.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	81 - 121	1e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	81 - 115	1.2e-06	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016750)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g07980
malus_domestica	MD05G1072100.v1.1 MD10G1082500.v1.1
rosa_chinensis	RchiOBHm_Chr6g0262431
rosa_laevigata	RLG00000014429
rosa_multiflora	Rmu_co8450695.1_g000001 Rmu_sc0010965.1_g000002 Rmu_sc0018995.1_g000001
rosa_roxburghii	Rroxscaffold_177G00434460 Rroxscaffold_7G00205270
rosa_rugosa	Rorug05G0597200
rosa_samantha	Rh6BG112000 Rh6CG110100 Rh6DG099700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	74
AasI	GACNNNNNNGTC	1 cut(s)	275
AccB7I	CCANNNNNTGG	1 cut(s)	354
AccI	GTMKAC	1 cut(s)	547
AciI	CCGC	2 cut(s)	198, 368
AclWI	GGATC	1 cut(s)	448
AcoI	YGGCCR	2 cut(s)	85, 289
AcsI	RAATTY	4 cut(s)	173, 246, 337, 343
AdeI	CACNNNGTG	3 cut(s)	83, 287, 425
AfaI	GTAC	2 cut(s)	553, 741
AfiI	CCNNNNNNNGG	2 cut(s)	354, 401
AflII	CTTAAG	1 cut(s)	478
AflIII	ACRYGT	1 cut(s)	508
AgsI	TTSAA	6 cut(s)	35, 127, 239, 316, 342, 506
AjiI	CACGTC	2 cut(s)	14, 434
AluBI	AGCT	2 cut(s)	530, 669
AluI	AGCT	2 cut(s)	530, 669
Alw21I	GWGCWC	1 cut(s)	532
Alw26I	GTCTC	3 cut(s)	261, 433, 488
AlwI	GGATC	1 cut(s)	448
Ama87I	CYCGRG	1 cut(s)	264
AoxI	GGCC	2 cut(s)	85, 289
ApoI	RAATTY	4 cut(s)	173, 246, 337, 343
AspLEI	GCGC	1 cut(s)	61
AspS9I	GGNCC	1 cut(s)	411
AsuC2I	CCSGG	1 cut(s)	438
AvaI	CYCGRG	1 cut(s)	264
AvaII	GGWCC	1 cut(s)	411
BaeGI	GKGCMC	1 cut(s)	152
BalI	TGGCCA	2 cut(s)	87, 291
BanII	GRGCYC	1 cut(s)	532
Bbv12I	GWGCWC	1 cut(s)	532
BcnI	CCSGG	1 cut(s)	438
BcoDI	GTCTC	3 cut(s)	261, 433, 488
BfrI	CTTAAG	1 cut(s)	478
Bme1390I	CCNGG	1 cut(s)	438
Bme18I	GGWCC	1 cut(s)	411
BmeT110I	CYCGRG	1 cut(s)	264
BmgBI	CACGTC	2 cut(s)	14, 434
BmgT120I	GGNCC	1 cut(s)	411
BmiI	GGNNCC	1 cut(s)	412
BmrFI	CCNGG	1 cut(s)	438
BmsI	GCATC	1 cut(s)	619
BpmI	CTGGAG	1 cut(s)	121
BpuMI	CCSGG	1 cut(s)	438
BsaI	GGTCTC	1 cut(s)	261
BsaJI	CCNNGG	2 cut(s)	555, 623
Bsc4I	CCNNNNNNNGG	2 cut(s)	354, 401
Bse118I	RCCGGY	1 cut(s)	467
Bse1I	ACTGG	2 cut(s)	169, 741
BseDI	CCNNGG	2 cut(s)	555, 623
BseGI	GGATG	3 cut(s)	385, 610, 706
BseLI	CCNNNNNNNGG	2 cut(s)	354, 401
BseNI	ACTGG	2 cut(s)	169, 741
BseSI	GKGCMC	1 cut(s)	152
BshFI	GGCC	2 cut(s)	87, 291
BsiHKAI	GWGCWC	1 cut(s)	532
BsiHKCI	CYCGRG	1 cut(s)	264
BsiSI	CCGG	2 cut(s)	437, 468
BslFI	GGGAC	2 cut(s)	377, 424
BslI	CCNNNNNNNGG	2 cut(s)	354, 401
BsmAI	GTCTC	3 cut(s)	261, 433, 488
BsmFI	GGGAC	2 cut(s)	377, 424
BsmI	GAATGC	2 cut(s)	41, 245
BsnI	GGCC	2 cut(s)	87, 291
Bso31I	GGTCTC	1 cut(s)	261
BsoBI	CYCGRG	1 cut(s)	264
Bsp1286I	GDGCHC	2 cut(s)	152, 532
Bsp143I	GATC	1 cut(s)	440
Bsp19I	CCATGG	1 cut(s)	555
BspACI	CCGC	2 cut(s)	198, 368
BspANI	GGCC	2 cut(s)	87, 291
BspLI	GGNNCC	1 cut(s)	412
BspPI	GGATC	1 cut(s)	448
BspTI	CTTAAG	1 cut(s)	478
BspTNI	GGTCTC	1 cut(s)	261
BsrFI	RCCGGY	1 cut(s)	467
BsrI	ACTGG	2 cut(s)	169, 741
BssAI	RCCGGY	1 cut(s)	467
BssECI	CCNNGG	2 cut(s)	555, 623
BssMI	GATC	1 cut(s)	440
BssT1I	CCWWGG	2 cut(s)	555, 623
Bst4CI	ACNGT	2 cut(s)	353, 457
BstAFI	CTTAAG	1 cut(s)	478
BstAPI	GCANNNNNTGC	2 cut(s)	45, 249
BstC8I	GCNNGC	1 cut(s)	469
BstDSI	CCRYGG	1 cut(s)	555
BstF5I	GGATG	3 cut(s)	385, 610, 706
BstHHI	GCGC	1 cut(s)	61
BstKTI	GATC	1 cut(s)	443
BstMAI	GTCTC	3 cut(s)	261, 433, 488
BstMBI	GATC	1 cut(s)	440
BstMWI	GCNNNNNNNGC	4 cut(s)	45, 249, 468, 473
BstSCI	CCNGG	1 cut(s)	436
BstSLI	GKGCMC	1 cut(s)	152
BsuRI	GGCC	2 cut(s)	87, 291
BtgI	CCRYGG	1 cut(s)	555
BtrI	CACGTC	2 cut(s)	14, 434
BtsCI	GGATG	3 cut(s)	385, 610, 706
BtsI	GCAGTG	2 cut(s)	462, 471
BtsIMutI	CAGTG	2 cut(s)	462, 471
Cac8I	GCNNGC	1 cut(s)	469
CfoI	GCGC	1 cut(s)	61
Cfr10I	RCCGGY	1 cut(s)	467
Cfr13I	GGNCC	1 cut(s)	411
Csp6I	GTAC	2 cut(s)	552, 740
CspCI	CAANNNNNGTGG	2 cut(s)	281, 316
CviAII	CATG	3 cut(s)	422, 444, 556
CviJI	RGCY	9 cut(s)	53, 87, 121, 180, 263, 291, 325, 530, 669
CviKI_1	RGCY	9 cut(s)	53, 87, 121, 180, 263, 291, 325, 530, 669
CviQI	GTAC	2 cut(s)	552, 740
DpnI	GATC	1 cut(s)	442
DpnII	GATC	1 cut(s)	440
DraIII	CACNNNGTG	3 cut(s)	83, 287, 425
DrdI	GACNNNNNNGTC	1 cut(s)	275
DseDI	GACNNNNNNGTC	1 cut(s)	275
EaeI	YGGCCR	2 cut(s)	85, 289
Ecl136II	GAGCTC	1 cut(s)	530
Eco130I	CCWWGG	2 cut(s)	555, 623
Eco24I	GRGCYC	1 cut(s)	532
Eco31I	GGTCTC	1 cut(s)	261
Eco47I	GGWCC	1 cut(s)	411
Eco53kI	GAGCTC	1 cut(s)	530
Eco88I	CYCGRG	1 cut(s)	264
EcoICRI	GAGCTC	1 cut(s)	530
EcoRI	GAATTC	2 cut(s)	173, 337
EcoT14I	CCWWGG	2 cut(s)	555, 623
EcoT38I	GRGCYC	1 cut(s)	532
ErhI	CCWWGG	2 cut(s)	555, 623
FaeI	CATG	3 cut(s)	425, 447, 559
FaqI	GGGAC	2 cut(s)	377, 424
FatI	CATG	3 cut(s)	421, 443, 555
FblI	GTMKAC	1 cut(s)	547
FokI	GGATG	3 cut(s)	392, 597, 713
FriOI	GRGCYC	1 cut(s)	532
GlaI	GCGC	1 cut(s)	60
GsuI	CTGGAG	1 cut(s)	121
HaeIII	GGCC	2 cut(s)	87, 291
HapII	CCGG	2 cut(s)	437, 468
HhaI	GCGC	1 cut(s)	61
Hin1II	CATG	3 cut(s)	425, 447, 559
Hin6I	GCGC	1 cut(s)	59
HinP1I	GCGC	1 cut(s)	59
HincII	GTYRAC	1 cut(s)	548
HindII	GTYRAC	1 cut(s)	548
HinfI	GANTC	1 cut(s)	220
HpaII	CCGG	2 cut(s)	437, 468
Hpy166II	GTNNAC	1 cut(s)	548
Hpy188I	TCNGA	1 cut(s)	256
Hpy188III	TCNNGA	1 cut(s)	485
Hpy8I	GTNNAC	1 cut(s)	548
Hpy99I	CGWCG	1 cut(s)	552
HpyAV	CCTTC	1 cut(s)	646
HpyCH4III	ACNGT	2 cut(s)	353, 457
HpyCH4IV	ACGT	4 cut(s)	13, 433, 508, 550
HpyCH4V	TGCA	6 cut(s)	39, 243, 363, 452, 593, 610
HpyF10VI	GCNNNNNNNGC	4 cut(s)	45, 249, 468, 473
HpySE526I	ACGT	4 cut(s)	13, 433, 508, 550
Hsp92II	CATG	3 cut(s)	425, 447, 559
HspAI	GCGC	1 cut(s)	59
KroI	GCCGGC	1 cut(s)	467
KroNI	GCCGGC	1 cut(s)	469
Kzo9I	GATC	1 cut(s)	440
LmnI	GCTCC	3 cut(s)	257, 527, 666
LpnPI	CCDG	7 cut(s)	81, 85, 94, 150, 450, 481, 722
LweI	GCATC	1 cut(s)	619
MaeII	ACGT	4 cut(s)	13, 433, 508, 550
MaeIII	GTNAC	2 cut(s)	277, 579
MalI	GATC	1 cut(s)	442
MboI	GATC	1 cut(s)	440
MfeI	CAATTG	1 cut(s)	273
MhlI	GDGCHC	2 cut(s)	152, 532
MlsI	TGGCCA	2 cut(s)	87, 291
MluNI	TGGCCA	2 cut(s)	87, 291
MmeI	TCCRAC	2 cut(s)	162, 372
MnlI	CCTC	4 cut(s)	101, 370, 405, 716
Mox20I	TGGCCA	2 cut(s)	87, 291
MroNI	GCCGGC	1 cut(s)	467
MscI	TGGCCA	2 cut(s)	87, 291
MseI	TTAA	4 cut(s)	327, 479, 720, 745
Msp20I	TGGCCA	2 cut(s)	87, 291
MspCI	CTTAAG	1 cut(s)	478
MspI	CCGG	2 cut(s)	437, 468
MspR9I	CCNGG	1 cut(s)	438
MunI	CAATTG	1 cut(s)	273
Mva1269I	GAATGC	2 cut(s)	41, 245
MwoI	GCNNNNNNNGC	4 cut(s)	45, 249, 468, 473
NaeI	GCCGGC	1 cut(s)	469
NciI	CCSGG	1 cut(s)	438
NcoI	CCATGG	1 cut(s)	555
NdeII	GATC	1 cut(s)	440
NgoMIV	GCCGGC	1 cut(s)	467
NlaIII	CATG	3 cut(s)	425, 447, 559
NlaIV	GGNNCC	1 cut(s)	412
NmuCI	GTSAC	1 cut(s)	277
PcsI	WCGNNNNNNNCGW	1 cut(s)	544
PctI	GAATGC	2 cut(s)	41, 245
PdiI	GCCGGC	1 cut(s)	469
PfeI	GAWTC	1 cut(s)	220
PflMI	CCANNNNNTGG	1 cut(s)	354
PfoI	TCCNGGA	1 cut(s)	436
PsiI	TTATAA	1 cut(s)	74
Psp124BI	GAGCTC	1 cut(s)	532
PspN4I	GGNNCC	1 cut(s)	412
PspPI	GGNCC	1 cut(s)	411
RsaI	GTAC	2 cut(s)	553, 741
RsaNI	GTAC	2 cut(s)	552, 740
SacI	GAGCTC	1 cut(s)	532
SalI	GTCGAC	1 cut(s)	546
SaqAI	TTAA	4 cut(s)	327, 479, 720, 745
Sau3AI	GATC	1 cut(s)	440
Sau96I	GGNCC	1 cut(s)	411
ScrFI	CCNGG	1 cut(s)	438
SduI	GDGCHC	2 cut(s)	152, 532
SetI	ASST	9 cut(s)	16, 93, 297, 436, 511, 532, 553, 657, 671
SfaNI	GCATC	1 cut(s)	619
SinI	GGWCC	1 cut(s)	411
SmlI	CTYRAG	1 cut(s)	478
SmoI	CTYRAG	1 cut(s)	478
SsiI	CCGC	2 cut(s)	198, 368
SstI	GAGCTC	1 cut(s)	532
StyD4I	CCNGG	1 cut(s)	436
StyI	CCWWGG	2 cut(s)	555, 623
TaaI	ACNGT	2 cut(s)	353, 457
TaiI	ACGT	4 cut(s)	16, 436, 511, 553
TaqI	TCGA	4 cut(s)	206, 218, 486, 547
TfiI	GAWTC	1 cut(s)	220
Tru1I	TTAA	4 cut(s)	327, 479, 720, 745
Tru9I	TTAA	4 cut(s)	327, 479, 720, 745
TscAI	CASTG	2 cut(s)	469, 478
TseFI	GTSAC	1 cut(s)	277
Tsp45I	GTSAC	1 cut(s)	277
TspDTI	ATGAA	2 cut(s)	128, 396
TspGWI	ACGGA	1 cut(s)	222
TspRI	CASTG	2 cut(s)	469, 478
Van91I	CCANNNNNTGG	1 cut(s)	354
Vha464I	CTTAAG	1 cut(s)	478
VpaK11BI	GGWCC	1 cut(s)	411
XapI	RAATTY	4 cut(s)	173, 246, 337, 343
XmiI	GTMKAC	1 cut(s)	547

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.