MD05G1072200.v1.1

Extra-large guanine nucleotide-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005580
Physical Location & Seq
Forward (+)
15186880 .. 15193315
6436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1072200.v1.1.491

Sequence Viewer

Length: 2727 bp
ATGGCTGCTGTGATGAGAAAACTGAGTTCTGCTATGGCGGCATCTGGTGGCGGCGACGGGGATGGTGGGGATTTTAGCATGGAGTTGGAGTTTTCGTTTGCCGTGGAGTACAAGGGTCCTCCGGTGGGTTATGATATCCCAAATGCATTTCCTGTTGATATTCGCCACATCCCAACTGCTGCTCCGGTTTCTTCAGCCTCTCTCATGAACAATTTGTCCCTCCCTGTGATTCATCCGATTTCAAAATCCACCAAGAAACCAAAAGAAGAGTCGAAACTTGAAACTGAGGTAAAGTTTGGAAAGGAAATTGTTTCTTCCAATTCAGTAACCCCTGCTGAATTGGAAATTAGCAATGGCGAAAAGGGTGTCTCGACTTTATCAGATAAAGGTGAAATCTCTGGGAAATTGGGGTCTTCTGGCAGTCTGTCTGGAAGTTTGGAGGGTGTGCTTGAATTGCCTGATGATGGTCAAGAAGCTCAAGGCTTTCCGAATTATATGAGCCCCGGTAATTGGGGTTCATCAGAGTCTGGTTCAAGTTCGAGGAGTCTTTCCTCTGAGGTTTTCTCTGGTAGAGAGGAAGCTGATACGGATGCAATTCCTCACCATGTCAAAAGGCCATCAGCTGTGACATTCCGTGATCCTGACTCAAATGACATTGTTCAGGAAGAAGAGTTTGATCTTTCTGAAGGGGAAGAAGATCTGCGAGTGAGGCCAACGATTGAGAGAAGTGGGAAGAAAGGGTCATGCTATCGATGTGGGAAAGGAAACAGACTCATGGAGAAGGAGGTTTGCATTGTTTGTGGTGCTAAGTATTGCTATAATTGTGTGCTAAGAGCAATGGGGTCAATGCCGGAAGGAAGGAAATGTGTTACTTGCATTAGTTTTGCAATTGACGAGTCAAGGCGAAGGAAACTGGGCAAGTGTTCTAGGATGTTGAAGTGGCTTCTCACTAAATCGGAAGTTGAACTGATAATGAAAGCCGAGATATCTTGCCAAGCAAATCAACTGCCAGGCAATCTTATTTTTGTGAATGGCGAACCTTTATCTCAGGAAGAGCTGGTTCGATTGCAAAGTTGCCGAAACCCACCAAAAAAGATAAAACCAGGATGCTATTGGTACGATAATGTATCTGGTTTTTGGGGAAAGGAAGGGCATAGGCCTTGCCAAATCATTAGCCCCCAGTTGAGTGTGGGAGGCCACATAAGGCGAAATGCTAGTAATGGGGATACAAATATACTGATTAACAATCGGGAGATCACAAAGTTAGAGGCCTTCATGCTGAAGTTGGCTGGAGTGCCATGTGAAGGAAATCTTCACTACTGGGTGAGTGCAGATGGGGAGTATCAGGAAGAGGGAATGAATCAAGTAAAGGGAAAGATATGGGACAAGACTACAATAAAGATGGTTTGTAACATCTTGTCTTTGCCAATTCCTTCTGGTTCTGCAAATCTTTCCACTGACGAAGTGAACACCCGGAACACCTTTGAAGAGAAAAAGCTTAACAAAATTGTTCTAGTTGGACATCACAAATCTGGTACAAGCACTATATTTAAACAGGCCAAGCTCTTGTACAATGTTCCTTTCTCTGAAGATGAGCGCCAAAGTATTAAGTTCGTGATCCAGAGCAGATTGTACAGTTATCTTGGTATATTGCTTGAAGGGCGTGAGTGGTTTGAAGAAGAAAGTTTGATTGAGAAGAGAAAAGGAAAAGGACGGCTTTTGGATGAACCTGGCCCTTCAGGGAATACAAGCCAGCTTGACGATAAAACAAGATATTCCATTGGTCCAAGACTGAAAGCTTTTTCTGATTGGCTCCTCAAATCTATGGTATCTGGTAATCTGGAAGCCATATTTCCAGCAGCTACCCGTGAATATGCACCATTTGTTGAGGATTTGTGGAAGGATCCTGCTATTCAATCCACATACGACCGGAGGAATGAAATAGAAATGCTGCCCAGACCAGCTGCATATTTTCTTAATCGGGCTGTTGAGATTTCAAGGACAGATTATGAGCCCACAGATATGGACATCTTGTATGCCGAGGGAATCACCTCATCCAACAGCCTTACGAGCATGGAATTCTCAATTCCCCCATCAGCTGGAAATAGCAATCTGGACCCTCCTTATCAGCATGACCCTTCGTTAAGGTACCAACTAATTAGAGTGCATCAGAGCACCCTCGGAGGAAACTGCAAGTTGGTCGAGATGTTTGAAGATGTTGACATGGTCGCATTTTGTGTTTCATTGACAGAATACGATGAGTTTTCTACAGATGTTAACGGGGTTTCCATAAACAAGATGATGGCAAGCAAGCAGCTATTTGAAAATATTGTTACTCATCCAGCCTTTGACCAAAAAGGCTTCCTTCTGATACTCAACAAGTATGATCTGCTCGAAGAAAAGATTGACGAAGCCCCTCTCACTCGATGTGAATGGTTTCATGACTTCAATCCGGTAACCAGCCAAAATCCCAGTAGTAGCAATAACAACAGCAATAACCCCACGTTGGCTCACCGTGCCTTCCAATATATTGCAGTGAAGTTCAAAAGATTCTTTCATTCTCTAACAGATAGGAAGCTGTTTGTTTCACTGGTTACAGCGCTAGAGTCTGATACTGTAGATGAAGCTCTTAGATATGCTAGTGAGATTCTAAAATGGGAGGAAGAGGTACCCAGATTAATTAACGAGATCTCTTCCGCTAGCATCGAGGCAAGTTCTTCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009898 GO:0009987 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200
Pfam Domains
Protein Families
G-alpha

Protein Analysis

909

Amino Acids

101.0

Weight (kDa)

5.36

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 491 - 882 5.8e-65 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 2148, 2668
AccB1I GGYRCC 2 cut(s) 2148, 2668
AccB7I CCANNNNNTGG 1 cut(s) 2099
AciI CCGC 4 cut(s) 38, 51, 2697, 2721
AclWI GGATC 4 cut(s) 632, 1612, 1898, 1911
AcsI RAATTY 1 cut(s) 2078
AcuI CTGAAG 5 cut(s) 177, 705, 1301, 1608, 1722
AfaI GTAC 7 cut(s) 110, 1118, 1537, 1571, 1634, 2150, 2670
AfeI AGCGCT 1 cut(s) 2601
AfiI CCNNNNNNNGG 6 cut(s) 125, 464, 510, 1304, 2099, 2506
AjnI CCWGG 3 cut(s) 1009, 1102, 1729
AloI GAACNNNNNNTCC 2 cut(s) 200, 232
Alw21I GWGCWC 1 cut(s) 2177
Alw26I GTCTC 1 cut(s) 373
AlwI GGATC 4 cut(s) 632, 1612, 1898, 1911
AlwNI CAGNNNCTG 1 cut(s) 527
Aor51HI AGCGCT 1 cut(s) 2601
AoxI GGCC 7 cut(s) 614, 710, 1157, 1195, 1269, 1557, 1732
ApeKI GCWGC 6 cut(s) 5, 179, 1859, 1951, 1964, 2314
ApoI RAATTY 1 cut(s) 2078
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 351, 383
AseI ATTAAT 1 cut(s) 2678
Asp700I GAANNNNTTC 1 cut(s) 2436
Asp718I GGTACC 2 cut(s) 2148, 2668
AspLEI GCGC 2 cut(s) 1599, 2602
AspS9I GGNCC 4 cut(s) 116, 1733, 1784, 2116
AsuC2I CCSGG 2 cut(s) 504, 1474
AsuHPI GGTGA 5 cut(s) 401, 593, 1336, 2041, 2504
AsuNHI GCTAGC 1 cut(s) 2699
AvaII GGWCC 3 cut(s) 116, 1784, 2116
BamHI GGATCC 1 cut(s) 1903
BanI GGYRCC 2 cut(s) 2148, 2668
BanII GRGCYC 2 cut(s) 503, 2016
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 2177
BbvI GCAGC 5 cut(s) 166, 1871, 1938, 1951, 2326
BccI CCATC 7 cut(s) 56, 458, 625, 1328, 1396, 2101, 2296
BceAI ACGGC 2 cut(s) 86, 1730
BcgI CGANNNNNNTGC 2 cut(s) 2182, 2216
BciT130I CCWGG 3 cut(s) 1011, 1104, 1731
BciVI GTATCC 1 cut(s) 1219
BcnI CCSGG 2 cut(s) 504, 1474
BcoDI GTCTC 1 cut(s) 373
BfaI CTAG 6 cut(s) 927, 1215, 1514, 2603, 2640, 2700
BfmI CTRYAG 2 cut(s) 2268, 2616
BfoI RGCGCY 2 cut(s) 1600, 2603
BfuI GTATCC 1 cut(s) 1219
BglII AGATCT 2 cut(s) 697, 2688
BisI GCNGC 8 cut(s) 6, 39, 52, 180, 1860, 1952, 1965, 2315
BlsI GCNGC 8 cut(s) 7, 40, 53, 181, 1861, 1953, 1966, 2316
Bme1390I CCNGG 5 cut(s) 504, 1011, 1104, 1474, 1731
Bme18I GGWCC 3 cut(s) 116, 1784, 2116
BmgT120I GGNCC 4 cut(s) 116, 1733, 1784, 2116
BmiI GGNNCC 6 cut(s) 117, 1814, 1905, 2118, 2150, 2670
BmrFI CCNGG 5 cut(s) 504, 1011, 1104, 1474, 1731
BmrI ACTGGG 4 cut(s) 923, 1174, 1330, 2466
BmsI GCATC 5 cut(s) 50, 580, 1097, 2176, 2712
BmtI GCTAGC 1 cut(s) 2703
BmuI ACTGGG 4 cut(s) 923, 1174, 1330, 2466
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpmI CTGGAG 1 cut(s) 1311
BpuEI CTTGAG 1 cut(s) 462
BpuMI CCSGG 2 cut(s) 504, 1474
Bsa29I ATCGAT 1 cut(s) 751
BsaJI CCNNGG 4 cut(s) 102, 502, 2040, 2179
BsaWI WCCGGW 4 cut(s) 121, 184, 1929, 2452
BsaXI ACNNNNNCTCC 6 cut(s) 74, 80, 104, 110, 166, 196
Bsc4I CCNNNNNNNGG 6 cut(s) 125, 464, 510, 1304, 2099, 2506
Bse1I ACTGG 5 cut(s) 918, 1180, 1325, 2472, 2595
Bse3DI GCAATG 2 cut(s) 358, 843
BseBI CCWGG 3 cut(s) 1011, 1104, 1731
BseCI ATCGAT 1 cut(s) 751
BseDI CCNNGG 4 cut(s) 102, 502, 2040, 2179
BseGI GGATG 9 cut(s) 67, 168, 232, 595, 936, 1112, 1729, 2054, 2338
BseLI CCNNNNNNNGG 6 cut(s) 125, 464, 510, 1304, 2099, 2506
BseMI GCAATG 2 cut(s) 358, 843
BseMII CTCAG 4 cut(s) 14, 276, 546, 1061
BseNI ACTGG 5 cut(s) 918, 1180, 1325, 2472, 2595
BseRI GAGGAG 2 cut(s) 556, 1805
BseXI GCAGC 5 cut(s) 166, 1871, 1938, 1951, 2326
BsgI GTGCAG 1 cut(s) 1350
Bsh1285I CGRYCG 1 cut(s) 1930
BshFI GGCC 7 cut(s) 616, 712, 1159, 1197, 1271, 1559, 1734
BshNI GGYRCC 2 cut(s) 2148, 2668
BshVI ATCGAT 1 cut(s) 751
BsiEI CGRYCG 1 cut(s) 1930
BsiHKAI GWGCWC 1 cut(s) 2177
BsiSI CCGG 7 cut(s) 122, 185, 504, 851, 1474, 1930, 2453
BslFI GGGAC 2 cut(s) 202, 1397
BslI CCNNNNNNNGG 6 cut(s) 125, 464, 510, 1304, 2099, 2506
BsmAI GTCTC 1 cut(s) 373
BsmFI GGGAC 2 cut(s) 202, 1397
BsnI GGCC 7 cut(s) 616, 712, 1159, 1197, 1271, 1559, 1734
Bsp1286I GDGCHC 3 cut(s) 503, 2016, 2177
Bsp1407I TGTACA 2 cut(s) 1569, 1632
Bsp143I GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
BspACI CCGC 4 cut(s) 38, 51, 2697, 2721
BspANI GGCC 7 cut(s) 616, 712, 1159, 1197, 1271, 1559, 1734
BspCNI CTCAG 4 cut(s) 15, 277, 547, 1060
BspDI ATCGAT 1 cut(s) 751
BspHI TCATGA 2 cut(s) 204, 2440
BspLI GGNNCC 6 cut(s) 117, 1814, 1905, 2118, 2150, 2670
BspOI GCTAGC 1 cut(s) 2703
BspPI GGATC 4 cut(s) 632, 1612, 1898, 1911
BspQI GCTCTTC 1 cut(s) 1047
BspT107I GGYRCC 2 cut(s) 2148, 2668
BsrDI GCAATG 2 cut(s) 358, 843
BsrGI TGTACA 2 cut(s) 1569, 1632
BsrI ACTGG 5 cut(s) 918, 1180, 1325, 2472, 2595
BssECI CCNNGG 4 cut(s) 102, 502, 2040, 2179
BssMI GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
Bst2UI CCWGG 3 cut(s) 1011, 1104, 1731
Bst4CI ACNGT 3 cut(s) 1637, 2516, 2617
Bst6I CTCTTC 8 cut(s) 261, 663, 1047, 1344, 1482, 1691, 2658, 2698
BstAUI TGTACA 2 cut(s) 1569, 1632
BstC8I GCNNGC 4 cut(s) 1754, 2308, 2312, 2701
BstDEI CTNAG 7 cut(s) 23, 285, 555, 807, 830, 1047, 2630
BstDSI CCRYGG 1 cut(s) 102
BstEII GGTNACC 1 cut(s) 2455
BstF5I GGATG 9 cut(s) 67, 168, 232, 595, 936, 1112, 1729, 2054, 2338
BstH2I RGCGCY 2 cut(s) 1600, 2603
BstHHI GCGC 2 cut(s) 1599, 2602
BstKTI GATC 8 cut(s) 640, 679, 700, 1257, 1620, 1906, 2389, 2691
BstMAI GTCTC 1 cut(s) 373
BstMBI GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
BstMCI CGRYCG 1 cut(s) 1930
BstMWI GCNNNNNNNGC 6 cut(s) 38, 454, 709, 1660, 2070, 2516
BstNI CCWGG 3 cut(s) 1011, 1104, 1731
BstPI GGTNACC 1 cut(s) 2455
BstSCI CCNGG 5 cut(s) 502, 1009, 1102, 1472, 1729
BstSFI CTRYAG 2 cut(s) 2268, 2616
BstV1I GCAGC 5 cut(s) 166, 1871, 1938, 1951, 2326
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 3 cut(s) 697, 1903, 2688
BstXI CCANNNNNNTGG 1 cut(s) 2023
BstYI RGATCY 3 cut(s) 697, 1903, 2688
Bsu15I ATCGAT 1 cut(s) 751
BsuI GTATCC 1 cut(s) 1219
BsuRI GGCC 7 cut(s) 616, 712, 1159, 1197, 1271, 1559, 1734
BsuTUI ATCGAT 1 cut(s) 751
BtgI CCRYGG 1 cut(s) 102
BtsCI GGATG 9 cut(s) 67, 168, 232, 595, 936, 1112, 1729, 2054, 2338
BtsI GCAGTG 1 cut(s) 2541
BtsIMutI CAGTG 3 cut(s) 1455, 2541, 2588
Cac8I GCNNGC 4 cut(s) 1754, 2308, 2312, 2701
CaiI CAGNNNCTG 1 cut(s) 527
CciI TCATGA 2 cut(s) 204, 2440
CfoI GCGC 2 cut(s) 1599, 2602
Cfr13I GGNCC 4 cut(s) 116, 1733, 1784, 2116
ClaI ATCGAT 1 cut(s) 751
Csp6I GTAC 7 cut(s) 109, 1117, 1536, 1570, 1633, 2149, 2669
CviQI GTAC 7 cut(s) 109, 1117, 1536, 1570, 1633, 2149, 2669
DdeI CTNAG 7 cut(s) 23, 285, 555, 807, 830, 1047, 2630
DpnI GATC 8 cut(s) 639, 678, 699, 1256, 1619, 1905, 2388, 2690
DpnII GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
DraI TTTAAA 1 cut(s) 1552
Eam1104I CTCTTC 8 cut(s) 261, 663, 1047, 1344, 1482, 1691, 2658, 2698
EarI CTCTTC 8 cut(s) 261, 663, 1047, 1344, 1482, 1691, 2658, 2698
Eco147I AGGCCT 2 cut(s) 1159, 1271
Eco24I GRGCYC 2 cut(s) 503, 2016
Eco32I GATATC 2 cut(s) 136, 987
Eco47I GGWCC 3 cut(s) 116, 1784, 2116
Eco47III AGCGCT 1 cut(s) 2601
Eco57I CTGAAG 5 cut(s) 177, 705, 1301, 1608, 1722
Eco91I GGTNACC 1 cut(s) 2455
EcoO109I RGGNCCY 1 cut(s) 116
EcoO65I GGTNACC 1 cut(s) 2455
EcoRI GAATTC 1 cut(s) 2078
EcoRII CCWGG 3 cut(s) 1009, 1102, 1729
EcoRV GATATC 2 cut(s) 136, 987
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 503, 2016
FalI AAGNNNNNCTT 6 cut(s) 1296, 1328, 1701, 1733, 2349, 2381
FaqI GGGAC 2 cut(s) 202, 1397
Fnu4HI GCNGC 8 cut(s) 6, 39, 52, 180, 1860, 1952, 1965, 2315
FokI GGATG 9 cut(s) 74, 155, 219, 602, 943, 1119, 1736, 2041, 2325
FriOI GRGCYC 2 cut(s) 503, 2016
Fsp4HI GCNGC 8 cut(s) 6, 39, 52, 180, 1860, 1952, 1965, 2315
FspBI CTAG 6 cut(s) 927, 1215, 1514, 2603, 2640, 2700
GlaI GCGC 2 cut(s) 1598, 2601
GluI GCNGC 8 cut(s) 6, 39, 52, 180, 1860, 1952, 1965, 2315
GsuI CTGGAG 1 cut(s) 1311
HaeII RGCGCY 2 cut(s) 1600, 2603
HaeIII GGCC 7 cut(s) 616, 712, 1159, 1197, 1271, 1559, 1734
HapII CCGG 7 cut(s) 122, 185, 504, 851, 1474, 1930, 2453
HhaI GCGC 2 cut(s) 1599, 2602
Hin6I GCGC 2 cut(s) 1597, 2600
HinP1I GCGC 2 cut(s) 1597, 2600
HincII GTYRAC 2 cut(s) 2221, 2278
HindII GTYRAC 2 cut(s) 2221, 2278
HindIII AAGCTT 2 cut(s) 1496, 1797
HpaI GTTAAC 1 cut(s) 2278
HpaII CCGG 7 cut(s) 122, 185, 504, 851, 1474, 1930, 2453
HphI GGTGA 5 cut(s) 401, 593, 1336, 2041, 2504
Hpy166II GTNNAC 3 cut(s) 1468, 2221, 2278
Hpy8I GTNNAC 3 cut(s) 1468, 2221, 2278
Hpy99I CGWCG 1 cut(s) 59
HpyCH4III ACNGT 3 cut(s) 1637, 2516, 2617
HpyCH4IV ACGT 1 cut(s) 2504
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 454, 709, 1660, 2070, 2516
HpyF3I CTNAG 7 cut(s) 23, 285, 555, 807, 830, 1047, 2630
HpySE526I ACGT 1 cut(s) 2504
HspAI GCGC 2 cut(s) 1597, 2600
KpnI GGTACC 2 cut(s) 2152, 2672
KspAI GTTAAC 1 cut(s) 2278
Kzo9I GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
LguI GCTCTTC 1 cut(s) 1047
LmnI GCTCC 2 cut(s) 187, 1818
Lsp1109I GCAGC 5 cut(s) 166, 1871, 1938, 1951, 2326
LweI GCATC 5 cut(s) 50, 580, 1097, 2176, 2712
MaeI CTAG 6 cut(s) 927, 1215, 1514, 2603, 2640, 2700
MaeII ACGT 1 cut(s) 2504
MaeIII GTNAC 7 cut(s) 325, 625, 868, 1409, 2332, 2455, 2593
MalI GATC 8 cut(s) 639, 678, 699, 1256, 1619, 1905, 2388, 2690
MboI GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 3 cut(s) 697, 1903, 2688
MhlI GDGCHC 3 cut(s) 503, 2016, 2177
MlyI GAGTC 7 cut(s) 278, 533, 553, 638, 765, 905, 2615
MmeI TCCRAC 3 cut(s) 66, 1498, 2082
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 2436
MslI CAYNNNNRTG 1 cut(s) 2021
MspA1I CMGCKG 3 cut(s) 623, 1964, 2099
MspI CCGG 7 cut(s) 122, 185, 504, 851, 1474, 1930, 2453
MspR9I CCNGG 5 cut(s) 504, 1011, 1104, 1474, 1731
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 3 cut(s) 1011, 1104, 1731
MwoI GCNNNNNNNGC 6 cut(s) 38, 454, 709, 1660, 2070, 2516
NciI CCSGG 2 cut(s) 504, 1474
NdeII GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
NheI GCTAGC 1 cut(s) 2699
NlaIV GGNNCC 6 cut(s) 117, 1814, 1905, 2118, 2150, 2670
NmeAIII GCCGAG 2 cut(s) 1006, 2065
NmuCI GTSAC 1 cut(s) 625
NsiI ATGCAT 1 cut(s) 148
PacI TTAATTAA 1 cut(s) 2682
PagI TCATGA 2 cut(s) 204, 2440
PceI AGGCCT 2 cut(s) 1159, 1271
PciSI GCTCTTC 1 cut(s) 1047
PdmI GAANNNNTTC 1 cut(s) 2436
PfeI GAWTC 5 cut(s) 229, 1360, 2046, 2550, 2647
PflFI GACNNNGTC 1 cut(s) 2225
PflMI CCANNNNNTGG 1 cut(s) 2099
PkrI GCNGC 8 cut(s) 7, 40, 53, 181, 1861, 1953, 1966, 2316
PleI GAGTC 7 cut(s) 277, 532, 552, 638, 765, 904, 2614
PpsI GAGTC 7 cut(s) 277, 532, 552, 638, 765, 904, 2614
PpuMI RGGWCCY 1 cut(s) 116
PshBI ATTAAT 1 cut(s) 2678
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 3 cut(s) 1009, 1102, 1729
PspEI GGTNACC 1 cut(s) 2455
PspGI CCWGG 3 cut(s) 1009, 1102, 1729
PspN4I GGNNCC 6 cut(s) 117, 1814, 1905, 2118, 2150, 2670
PspPI GGNCC 4 cut(s) 116, 1733, 1784, 2116
PspPPI RGGWCCY 1 cut(s) 116
PsrI GAACNNNNNNTAC 2 cut(s) 499, 531
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 3 cut(s) 697, 1903, 2688
PsyI GACNNNGTC 1 cut(s) 2225
PvuII CAGCTG 3 cut(s) 623, 1964, 2099
RsaI GTAC 7 cut(s) 110, 1118, 1537, 1571, 1634, 2150, 2670
RsaNI GTAC 7 cut(s) 109, 1117, 1536, 1570, 1633, 2149, 2669
RseI CAYNNNNRTG 1 cut(s) 2021
SapI GCTCTTC 1 cut(s) 1047
SatI GCNGC 8 cut(s) 6, 39, 52, 180, 1860, 1952, 1965, 2315
Sau3AI GATC 8 cut(s) 637, 676, 697, 1254, 1617, 1903, 2386, 2688
Sau96I GGNCC 4 cut(s) 116, 1733, 1784, 2116
SchI GAGTC 7 cut(s) 278, 533, 553, 638, 765, 905, 2615
ScrFI CCNGG 5 cut(s) 504, 1011, 1104, 1474, 1731
SduI GDGCHC 3 cut(s) 503, 2016, 2177
SfaNI GCATC 5 cut(s) 50, 580, 1097, 2176, 2712
SfcI CTRYAG 2 cut(s) 2268, 2616
SinI GGWCC 3 cut(s) 116, 1784, 2116
SmiMI CAYNNNNRTG 1 cut(s) 2021
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
SseBI AGGCCT 2 cut(s) 1159, 1271
SsiI CCGC 4 cut(s) 38, 51, 2697, 2721
SspI AATATT 1 cut(s) 2329
SspMI CTAG 6 cut(s) 927, 1215, 1514, 2603, 2640, 2700
StuI AGGCCT 2 cut(s) 1159, 1271
StyD4I CCNGG 5 cut(s) 502, 1009, 1102, 1472, 1729
TaaI ACNGT 3 cut(s) 1637, 2516, 2617
TaiI ACGT 1 cut(s) 2507
TaqI TCGA 9 cut(s) 272, 371, 539, 751, 1063, 2202, 2394, 2425, 2706
TatI WGTACW 3 cut(s) 108, 1569, 1632
TauI GCSGC 2 cut(s) 41, 54
TfiI GAWTC 5 cut(s) 229, 1360, 2046, 2550, 2647
TscAI CASTG 3 cut(s) 1462, 2541, 2595
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 6 cut(s) 5, 179, 1859, 1951, 1964, 2314
Tsp45I GTSAC 1 cut(s) 625
TspGWI ACGGA 2 cut(s) 602, 623
TspRI CASTG 3 cut(s) 1462, 2541, 2595
Tth111I GACNNNGTC 1 cut(s) 2225
Van91I CCANNNNNTGG 1 cut(s) 2099
VpaK11BI GGWCC 3 cut(s) 116, 1784, 2116
VspI ATTAAT 1 cut(s) 2678
XapI RAATTY 1 cut(s) 2078
XcmI CCANNNNNNNNNTGG 1 cut(s) 1110
XmnI GAANNNNTTC 1 cut(s) 2436
XspI CTAG 6 cut(s) 927, 1215, 1514, 2603, 2640, 2700
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.