Rroxscaffold_7G00207420

Extra-large guanine nucleotide-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005580
Physical Location & Seq
Reverse (-)
57295694 .. 57302111
6418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00207420.1

Sequence Viewer

Length: 2634 bp
ATGGCTGCTGCGGTTATGAGGAAGCTGAGTGCTGCTTTGGCTTCAAAAGGTGCTGATTTTGATGTCGAGAGTTACGAGGATTTGAGCGCGGAGTTGGAATACTCGTTTGCAGTGGAGTATCAAGGTCCTCCGGTGGGTTATGAAATCCCACATGCGTTTCCGATTGACATCCACCGAATCCCGACTGCTGCTCCGGTTTCTTCACCGTCTCTGTTGCACAATCTGTCGCTGCCGATCATCCAGCCCATTGCGAAGTCCAGTCAGAGCAGGAAGGCCAGAGTAGAGACTAAACTTGGGGATGAAGTAAAGGCGGTTCTTGATTCAGGTGAAGAGAAGGGTGTGTGTAGTGAAGTTGGGAGCTCTGGTGAATTGGGTTGTGGAAAGTTGGAGGGTGAGGTGGAGTTGGGTGAGGATGGTGGAGAAGGTGATTGGGGATCAACAGAGTCTGGGTTGAGCTCAAGGAGTGTTTCATCTGAGGTTTTCTCTGGCAGAGAGGAAGCTGGTGTGGATGAGATTGTTCCTCACCATGTGAAAAGGCCATCGACTGTGACTTTTCGCGATCCGGATTCAAATGACATTGTTGTGCAGGAAGTGCAGTTCAATGCTTCTGATGGGGATGAGAGTTTGCAAGTGAGGCCGAGGGTGGAGAGGAATGGGAAGAAGGGGTCGTGCTATCGATGTGGGAAAGGGAACAAGTTCACTGAGAAGGAGGTGTGCATTGTTTGTGGTGCAAAGTATTGCTTTGATTGTGTGCTGAGGGCAATGGGGTCAATGCCGGAAGGAAGGAAATGTGTTACTTGCATTCGGTTTGGGATTGATGAGTCGAGGCGTGGGAGTATTGGCAAGTCTTCTAGGATGCTCAGGCGGCTTCTGACTAAGGCGCAAGTGGAAGTGGTCATGAAAGCTGAGATATCATGCCAAGCAAATCAACTGCCAGGCAATCTTGTCTTCGTGAACGACGAGCCTCTTACACAAGAAGAGCTCTCTAGATTGCAAGGTTGCAGAAACCCTCCAAAAAATCTAAAACCAGGATCTTATTGGTATGATAATGTATCTGGTTTTTGGGGAAAGGTTGGGCGTAGGCCTTGCCAAGTTATATGCCCCCAGCTGAATGTTGGGGGCCATTTAAGGGAAAATGCTAGTAATGGGGACACAAAAATAATGATAAACGGTCGAGTGATCACCAAGTTAGAGGTCTTCATGCTGCAGATGGCAGGAGTGCCATGTGAAGGAAAACTCAACTACTGGGTGAATGCAGATGGATCCTATCAGGAAGAGGGAATGAATAACGTCAAAGGAAAGATATGGGACATGAAAATAAAGCTGGTTTGCACTATCTTGTCTCTACCAATTCCTTCTGAACCTACAATTCCTCCTCCTGCGGAAGAAGACAGAAACTTTATTGAAGAGAAACTGCTCGACAAACTTGTTTTAGTTGGGTATCATAAATCTGGCTCAAGCACTATATTCAAACAGGCAAAGTTTTTGTATAATGTTCCTTTTTCTGAAGATGAGCGCCAAAATATTAAGTTCATGATCCAAAGCAGATTGTACAGTTATCTTGGTATCCTGCTTGAGGGGCGTGAATGGTTTGAAGAAGAAAGTTTGGTTGAAAACAGAAAAGGACAGCTTCGAGATGAACCTGGTCCTTCAGGAATTGCAAGCCAGCTGGACAGTAAAACAACGTATGCCATTGGCCCGAGACTGAAATCTTTTGCAGATTGGCTACTAAAGAATATGATATCAGGTACTTTGGAAGCCATATTTCCTGCGGCTACTCGTGAATATGCACCGTTTGTTGAGGAGTTGTGGAATGATCCAGCTATTCAAGCCACATACAACCGGAGGAATGAATTAGAAATGCTACCCAGACAGGCTACATATTTTCTTAATCGGGCTGTAGAAATTTCAAGGACCGATTATGAGCCCTCAGACATGGACATCTTGTACGCAGAGGGAATTACCTCGTCCAACAGCCTTAATACCATGGAATTTTCATTGACTAGATCATCTCAACGTAGCGATCTGGACTCTATCTATCAGCTTAATCCTTCGCAAAGGTATCAGCTCATTAGAGTGCATAGCCTTGGAGGAAGTTGCAAGTTGCTGGAGATGTTTGAAGATGTTGATATGGTCATATTTTGTGTTGCTTTGACAGACTACGATGAGTTTTGTGAAGATGCTAGTGGGGTATCCATAAACAAGATGATGGCAAGTAAGCAGCTGTTTGAAAGCATTGCAACTCATCCGGCTTTTGATCAGAAACACTTCCTTCTGATACTCAACAAGTTTGATCTGCTCGAAGAAAAGATTGACCAAGTCCCTCTCACTCGATGCGAATGGTTTAATGACTTCAATCCAGCAACCAGTCATAATCCCAACAGTAGTAATAATAACAGTAATAGTCCTACACTGGCTCATCGAGCCTTTCAGTATATTGCAATGAAGTTCAAAAGGCTATTTCATACCCAAACAGATAGAAAGTTGTTTGTTTCCCCAGTTACTGCATTAGAGCCTGATACTGTAGATGAAGCTCTCAGATATGCTAGTGAGATTCTAAGATGTGAGGAAGAGGTACCCAGATTAATTAATGAGTTCTCTTCGGCTAGCATTGATGCCAGTTCTACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009898 GO:0009987 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200
Pfam Domains
Protein Families
G-alpha

Protein Analysis

877

Amino Acids

97.97

Weight (kDa)

5.2

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 475 - 851 3.1e-64 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2575
AccB1I GGYRCC 1 cut(s) 2575
AccII CGCG 2 cut(s) 89, 558
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 6 cut(s) 11, 89, 311, 865, 1382, 1772
AclWI GGATC 7 cut(s) 442, 554, 1039, 1257, 1270, 1531, 1811
AcsI RAATTY 2 cut(s) 1905, 1991
AcuI CTGAAG 2 cut(s) 1527, 1635
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 4 cut(s) 1553, 1750, 1949, 2577
AfiI CCNNNNNNNGG 4 cut(s) 134, 1129, 1229, 1576
AjnI CCWGG 3 cut(s) 934, 1027, 1642
AloI GAACNNNNNNTCC 2 cut(s) 581, 613
Alw21I GWGCWC 3 cut(s) 362, 458, 984
Alw26I GTCTC 4 cut(s) 213, 278, 1347, 1696
AlwI GGATC 7 cut(s) 442, 554, 1039, 1257, 1270, 1531, 1811
AlwNI CAGNNNCTG 2 cut(s) 446, 2504
Ama87I CYCGRG 1 cut(s) 1699
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 6 cut(s) 273, 536, 635, 1082, 1120, 1696
ApeKI GCWGC 7 cut(s) 5, 8, 32, 188, 229, 1204, 2221
ApoI RAATTY 2 cut(s) 1905, 1991
AseI ATTAAT 2 cut(s) 2585, 2589
Asp700I GAANNNNTTC 2 cut(s) 695, 2267
Asp718I GGTACC 1 cut(s) 2575
AspLEI GCGC 3 cut(s) 89, 883, 1518
AspS9I GGNCC 5 cut(s) 125, 1120, 1646, 1697, 1914
AsuHPI GGTGA 9 cut(s) 195, 338, 377, 404, 419, 437, 515, 1174, 1261
AsuNHI GCTAGC 1 cut(s) 2606
AvaI CYCGRG 1 cut(s) 1699
AvaII GGWCC 3 cut(s) 125, 1646, 1914
BamHI GGATCC 1 cut(s) 1262
BanI GGYRCC 1 cut(s) 2575
BanII GRGCYC 4 cut(s) 362, 458, 984, 1929
BauI CACGAG 1 cut(s) 1779
BbsI GAAGAC 4 cut(s) 840, 940, 1189, 1395
Bbv12I GWGCWC 3 cut(s) 362, 458, 984
BbvCI CCTCAGC 1 cut(s) 755
BbvI GCAGC 5 cut(s) 19, 175, 216, 1191, 2233
BccI CCATC 6 cut(s) 407, 547, 605, 1204, 1253, 2203
BciT130I CCWGG 3 cut(s) 936, 1029, 1644
BciVI GTATCC 2 cut(s) 1577, 2203
BclI TGATCA 2 cut(s) 1179, 2257
BcoDI GTCTC 4 cut(s) 213, 278, 1347, 1696
BfaI CTAG 7 cut(s) 852, 987, 1140, 2004, 2184, 2547, 2607
BfmI CTRYAG 4 cut(s) 1205, 1899, 2523, 2625
BfoI RGCGCY 1 cut(s) 1519
BfuI GTATCC 2 cut(s) 1577, 2203
BisI GCNGC 9 cut(s) 6, 9, 33, 189, 230, 866, 1205, 1773, 2222
BlsI GCNGC 9 cut(s) 7, 10, 34, 190, 231, 867, 1206, 1774, 2223
Bme1390I CCNGG 3 cut(s) 936, 1029, 1644
Bme18I GGWCC 3 cut(s) 125, 1646, 1914
BmeT110I CYCGRG 1 cut(s) 1699
BmgT120I GGNCC 5 cut(s) 125, 1120, 1646, 1697, 1914
BmiI GGNNCC 3 cut(s) 1121, 1264, 2577
BmrFI CCNGG 3 cut(s) 936, 1029, 1644
BmrI ACTGGG 2 cut(s) 1255, 2492
BmsI GCATC 4 cut(s) 846, 2170, 2324, 2605
BmtI GCTAGC 1 cut(s) 2610
BmuI ACTGGG 2 cut(s) 1255, 2492
BpiI GAAGAC 4 cut(s) 840, 940, 1189, 1395
BplI GAGNNNNNCTC 2 cut(s) 467, 499
BpmI CTGGAG 1 cut(s) 2129
Bpu10I CCTNAGC 2 cut(s) 755, 860
BpuEI CTTGAG 3 cut(s) 442, 1441, 1595
Bsa29I ATCGAT 1 cut(s) 676
BsaBI GATNNNNATC 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 638, 1986, 2086
BsaWI WCCGGW 4 cut(s) 130, 193, 562, 1842
BsaXI ACNNNNNCTCC 6 cut(s) 175, 205, 349, 379, 1357, 1387
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 1129, 1229, 1576
Bse1I ACTGG 6 cut(s) 258, 1250, 2367, 2418, 2498, 2619
Bse3DI GCAATG 4 cut(s) 246, 768, 2235, 2448
Bse8I GATNNNNATC 1 cut(s) 167
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 3 cut(s) 936, 1029, 1644
BseCI ATCGAT 1 cut(s) 676
BseDI CCNNGG 3 cut(s) 638, 1986, 2086
BseGI GGATG 8 cut(s) 168, 237, 304, 418, 514, 622, 861, 2245
BseJI GATNNNNATC 1 cut(s) 167
BseLI CCNNNNNNNGG 4 cut(s) 134, 1129, 1229, 1576
BseMI GCAATG 4 cut(s) 246, 768, 2235, 2448
BseMII CTCAG 8 cut(s) 17, 465, 693, 746, 874, 897, 1944, 2551
BseNI ACTGG 6 cut(s) 258, 1250, 2367, 2418, 2498, 2619
BseRI GAGGAG 2 cut(s) 1365, 1817
BseXI GCAGC 5 cut(s) 19, 175, 216, 1191, 2233
BseYI CCCAGC 1 cut(s) 1104
BsgI GTGCAG 2 cut(s) 605, 614
Bsh1236I CGCG 2 cut(s) 89, 558
Bsh1285I CGRYCG 1 cut(s) 1174
BshFI GGCC 6 cut(s) 275, 538, 637, 1084, 1122, 1698
BshNI GGYRCC 1 cut(s) 2575
BshVI ATCGAT 1 cut(s) 676
BsiEI CGRYCG 1 cut(s) 1174
BsiHKAI GWGCWC 3 cut(s) 362, 458, 984
BsiHKCI CYCGRG 1 cut(s) 1699
BsiSI CCGG 6 cut(s) 131, 194, 563, 776, 1843, 2249
BslFI GGGAC 3 cut(s) 1163, 1322, 2306
BslI CCNNNNNNNGG 4 cut(s) 134, 1129, 1229, 1576
BsmAI GTCTC 4 cut(s) 213, 278, 1347, 1696
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 3 cut(s) 1163, 1322, 2306
BsmI GAATGC 2 cut(s) 801, 1258
BsnI GGCC 6 cut(s) 275, 538, 637, 1084, 1122, 1698
BsoBI CYCGRG 1 cut(s) 1699
Bsp1286I GDGCHC 4 cut(s) 362, 458, 984, 1929
Bsp13I TCCGGA 1 cut(s) 562
Bsp1407I TGTACA 1 cut(s) 1551
Bsp19I CCATGG 1 cut(s) 1986
Bsp68I TCGCGA 1 cut(s) 558
BspACI CCGC 6 cut(s) 11, 89, 311, 865, 1382, 1772
BspANI GGCC 6 cut(s) 275, 538, 637, 1084, 1122, 1698
BspCNI CTCAG 8 cut(s) 18, 466, 694, 747, 873, 898, 1943, 2550
BspDI ATCGAT 1 cut(s) 676
BspEI TCCGGA 1 cut(s) 562
BspFNI CGCG 2 cut(s) 89, 558
BspHI TCATGA 2 cut(s) 897, 1533
BspLI GGNNCC 3 cut(s) 1121, 1264, 2577
BspMAI CTGCAG 1 cut(s) 1209
BspOI GCTAGC 1 cut(s) 2610
BspPI GGATC 7 cut(s) 442, 554, 1039, 1257, 1270, 1531, 1811
BspQI GCTCTTC 1 cut(s) 972
BspT107I GGYRCC 1 cut(s) 2575
BsrDI GCAATG 4 cut(s) 246, 768, 2235, 2448
BsrGI TGTACA 1 cut(s) 1551
BsrI ACTGG 6 cut(s) 258, 1250, 2367, 2418, 2498, 2619
BssECI CCNNGG 3 cut(s) 638, 1986, 2086
BssSI CACGAG 1 cut(s) 1779
BssT1I CCWWGG 2 cut(s) 1986, 2086
Bst2BI CACGAG 1 cut(s) 1779
Bst2UI CCWGG 3 cut(s) 936, 1029, 1644
Bst4CI ACNGT 9 cut(s) 207, 547, 1172, 1556, 1676, 1794, 2384, 2399, 2524
Bst6I CTCTTC 6 cut(s) 324, 972, 1269, 1401, 2565, 2605
BstAPI GCANNNNNTGC 1 cut(s) 592
BstAUI TGTACA 1 cut(s) 1551
BstC8I GCNNGC 3 cut(s) 1663, 1667, 2608
BstDSI CCRYGG 1 cut(s) 1986
BstENI CCTNNNNNAGG 1 cut(s) 1574
BstF5I GGATG 8 cut(s) 168, 237, 304, 418, 514, 622, 861, 2245
BstFNI CGCG 2 cut(s) 89, 558
BstH2I RGCGCY 1 cut(s) 1519
BstHHI GCGC 3 cut(s) 89, 883, 1518
BstMAI GTCTC 4 cut(s) 213, 278, 1347, 1696
BstMCI CGRYCG 1 cut(s) 1174
BstMWI GCNNNNNNNGC 7 cut(s) 38, 592, 634, 865, 1579, 1829, 2423
BstNI CCWGG 3 cut(s) 936, 1029, 1644
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 3 cut(s) 934, 1027, 1642
BstSFI CTRYAG 4 cut(s) 1205, 1899, 2523, 2625
BstUI CGCG 2 cut(s) 89, 558
BstV1I GCAGC 5 cut(s) 19, 175, 216, 1191, 2233
BstV2I GAAGAC 4 cut(s) 840, 940, 1189, 1395
BstX2I RGATCY 2 cut(s) 1031, 1262
BstYI RGATCY 2 cut(s) 1031, 1262
Bsu15I ATCGAT 1 cut(s) 676
BsuI GTATCC 2 cut(s) 1577, 2203
BsuRI GGCC 6 cut(s) 275, 538, 637, 1084, 1122, 1698
BsuTUI ATCGAT 1 cut(s) 676
BtgI CCRYGG 1 cut(s) 1986
BtsCI GGATG 8 cut(s) 168, 237, 304, 418, 514, 622, 861, 2245
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 3 cut(s) 117, 699, 2411
BtuMI TCGCGA 1 cut(s) 558
Cac8I GCNNGC 3 cut(s) 1663, 1667, 2608
CaiI CAGNNNCTG 2 cut(s) 446, 2504
CciI TCATGA 2 cut(s) 897, 1533
CfoI GCGC 3 cut(s) 89, 883, 1518
Cfr13I GGNCC 5 cut(s) 125, 1120, 1646, 1697, 1914
ClaI ATCGAT 1 cut(s) 676
CsiI ACCWGGT 1 cut(s) 1642
Csp6I GTAC 4 cut(s) 1552, 1749, 1948, 2576
CviQI GTAC 4 cut(s) 1552, 1749, 1948, 2576
DraIII CACNNNGTG 1 cut(s) 529
Eam1104I CTCTTC 6 cut(s) 324, 972, 1269, 1401, 2565, 2605
EarI CTCTTC 6 cut(s) 324, 972, 1269, 1401, 2565, 2605
Ecl136II GAGCTC 3 cut(s) 360, 456, 982
Eco130I CCWWGG 2 cut(s) 1986, 2086
Eco147I AGGCCT 1 cut(s) 1084
Eco24I GRGCYC 4 cut(s) 362, 458, 984, 1929
Eco32I GATATC 2 cut(s) 912, 1743
Eco47I GGWCC 3 cut(s) 125, 1646, 1914
Eco53kI GAGCTC 3 cut(s) 360, 456, 982
Eco57I CTGAAG 2 cut(s) 1527, 1635
Eco88I CYCGRG 1 cut(s) 1699
EcoICRI GAGCTC 3 cut(s) 360, 456, 982
EcoNI CCTNNNNNAGG 1 cut(s) 1574
EcoO109I RGGNCCY 1 cut(s) 125
EcoRII CCWGG 3 cut(s) 934, 1027, 1642
EcoRV GATATC 2 cut(s) 912, 1743
EcoT14I CCWWGG 2 cut(s) 1986, 2086
EcoT38I GRGCYC 4 cut(s) 362, 458, 984, 1929
ErhI CCWWGG 2 cut(s) 1986, 2086
Esp3I CGTCTC 1 cut(s) 213
FalI AAGNNNNNCTT 4 cut(s) 725, 757, 1614, 1646
FaqI GGGAC 3 cut(s) 1163, 1322, 2306
FbaI TGATCA 2 cut(s) 1179, 2257
Fnu4HI GCNGC 9 cut(s) 6, 9, 33, 189, 230, 866, 1205, 1773, 2222
FokI GGATG 8 cut(s) 155, 224, 311, 425, 521, 629, 868, 2232
FriOI GRGCYC 4 cut(s) 362, 458, 984, 1929
Fsp4HI GCNGC 9 cut(s) 6, 9, 33, 189, 230, 866, 1205, 1773, 2222
FspBI CTAG 7 cut(s) 852, 987, 1140, 2004, 2184, 2547, 2607
GlaI GCGC 3 cut(s) 88, 882, 1517
GluI GCNGC 9 cut(s) 6, 9, 33, 189, 230, 866, 1205, 1773, 2222
GsaI CCCAGC 1 cut(s) 1108
GsuI CTGGAG 1 cut(s) 2129
HaeII RGCGCY 1 cut(s) 1519
HaeIII GGCC 6 cut(s) 275, 538, 637, 1084, 1122, 1698
HapII CCGG 6 cut(s) 131, 194, 563, 776, 1843, 2249
HhaI GCGC 3 cut(s) 89, 883, 1518
Hin6I GCGC 3 cut(s) 87, 881, 1516
HinP1I GCGC 3 cut(s) 87, 881, 1516
HinfI GANTC 7 cut(s) 177, 320, 443, 566, 821, 2030, 2554
HpaII CCGG 6 cut(s) 131, 194, 563, 776, 1843, 2249
HphI GGTGA 9 cut(s) 195, 338, 377, 404, 419, 437, 515, 1174, 1261
Hpy166II GTNNAC 2 cut(s) 699, 955
Hpy8I GTNNAC 2 cut(s) 699, 955
Hpy99I CGWCG 1 cut(s) 962
HpyCH4III ACNGT 9 cut(s) 207, 547, 1172, 1556, 1676, 1794, 2384, 2399, 2524
HpyCH4IV ACGT 3 cut(s) 1290, 1685, 2017
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 592, 634, 865, 1579, 1829, 2423
HpySE526I ACGT 3 cut(s) 1290, 1685, 2017
HspAI GCGC 3 cut(s) 87, 881, 1516
Kpn2I TCCGGA 1 cut(s) 562
KpnI GGTACC 1 cut(s) 2579
Ksp22I TGATCA 2 cut(s) 1179, 2257
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 2 cut(s) 196, 357
Lsp1109I GCAGC 5 cut(s) 19, 175, 216, 1191, 2233
LweI GCATC 4 cut(s) 846, 2170, 2324, 2605
MabI ACCWGGT 1 cut(s) 1642
MaeI CTAG 7 cut(s) 852, 987, 1140, 2004, 2184, 2547, 2607
MaeII ACGT 3 cut(s) 1290, 1685, 2017
MaeIII GTNAC 4 cut(s) 71, 547, 793, 2500
MflI RGATCY 2 cut(s) 1031, 1262
MhlI GDGCHC 4 cut(s) 362, 458, 984, 1929
MluCI AATT 9 cut(s) 368, 1350, 1368, 1656, 1853, 1905, 1959, 1991, 2586
MlyI GAGTC 3 cut(s) 452, 830, 2024
MmeI TCCRAC 3 cut(s) 75, 366, 1995
MroI TCCGGA 1 cut(s) 562
MroXI GAANNNNTTC 2 cut(s) 695, 2267
MseI TTAA 9 cut(s) 1127, 1527, 1890, 1980, 2046, 2346, 2585, 2589, 2632
MslI CAYNNNNRTG 2 cut(s) 581, 2075
MspA1I CMGCKG 3 cut(s) 1108, 1669, 2224
MspI CCGG 6 cut(s) 131, 194, 563, 776, 1843, 2249
MspR9I CCNGG 3 cut(s) 936, 1029, 1644
Mva1269I GAATGC 2 cut(s) 801, 1258
MvaI CCWGG 3 cut(s) 936, 1029, 1644
MvnI CGCG 2 cut(s) 89, 558
MwoI GCNNNNNNNGC 7 cut(s) 38, 592, 634, 865, 1579, 1829, 2423
NcoI CCATGG 1 cut(s) 1986
NheI GCTAGC 1 cut(s) 2606
NlaIV GGNNCC 3 cut(s) 1121, 1264, 2577
NmeAIII GCCGAG 1 cut(s) 663
NmuCI GTSAC 1 cut(s) 547
NruI TCGCGA 1 cut(s) 558
NspI RCATGY 1 cut(s) 155
PacI TTAATTAA 1 cut(s) 2589
PagI TCATGA 2 cut(s) 897, 1533
PceI AGGCCT 1 cut(s) 1084
PciSI GCTCTTC 1 cut(s) 972
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 957
PctI GAATGC 2 cut(s) 801, 1258
PdmI GAANNNNTTC 2 cut(s) 695, 2267
PfeI GAWTC 4 cut(s) 177, 320, 566, 2554
PflFI GACNNNGTC 1 cut(s) 2318
PkrI GCNGC 9 cut(s) 7, 10, 34, 190, 231, 867, 1206, 1774, 2223
PleI GAGTC 3 cut(s) 451, 829, 2024
PpsI GAGTC 3 cut(s) 451, 829, 2024
PpuMI RGGWCCY 1 cut(s) 125
PshBI ATTAAT 2 cut(s) 2585, 2589
Psp124BI GAGCTC 3 cut(s) 362, 458, 984
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 3 cut(s) 934, 1027, 1642
PspFI CCCAGC 1 cut(s) 1104
PspGI CCWGG 3 cut(s) 934, 1027, 1642
PspN4I GGNNCC 3 cut(s) 1121, 1264, 2577
PspPI GGNCC 5 cut(s) 125, 1120, 1646, 1697, 1914
PspPPI RGGWCCY 1 cut(s) 125
PsrI GAACNNNNNNTAC 2 cut(s) 297, 329
PstI CTGCAG 1 cut(s) 1209
PstNI CAGNNNCTG 2 cut(s) 446, 2504
PsuI RGATCY 2 cut(s) 1031, 1262
PsyI GACNNNGTC 1 cut(s) 2318
PvuII CAGCTG 3 cut(s) 1108, 1669, 2224
RruI TCGCGA 1 cut(s) 558
RsaI GTAC 4 cut(s) 1553, 1750, 1949, 2577
RsaNI GTAC 4 cut(s) 1552, 1749, 1948, 2576
RseI CAYNNNNRTG 2 cut(s) 581, 2075
SacI GAGCTC 3 cut(s) 362, 458, 984
SapI GCTCTTC 1 cut(s) 972
SaqAI TTAA 9 cut(s) 1127, 1527, 1890, 1980, 2046, 2346, 2585, 2589, 2632
SatI GCNGC 9 cut(s) 6, 9, 33, 189, 230, 866, 1205, 1773, 2222
Sau96I GGNCC 5 cut(s) 125, 1120, 1646, 1697, 1914
SchI GAGTC 3 cut(s) 452, 830, 2024
ScrFI CCNGG 3 cut(s) 936, 1029, 1644
SduI GDGCHC 4 cut(s) 362, 458, 984, 1929
SexAI ACCWGGT 1 cut(s) 1642
SfaNI GCATC 4 cut(s) 846, 2170, 2324, 2605
SfcI CTRYAG 4 cut(s) 1205, 1899, 2523, 2625
SinI GGWCC 3 cut(s) 125, 1646, 1914
SmiMI CAYNNNNRTG 2 cut(s) 581, 2075
SmlI CTYRAG 3 cut(s) 457, 1456, 1574
SmoI CTYRAG 3 cut(s) 457, 1456, 1574
Sse9I AATT 9 cut(s) 368, 1350, 1368, 1656, 1853, 1905, 1959, 1991, 2586
SseBI AGGCCT 1 cut(s) 1084
SsiI CCGC 6 cut(s) 11, 89, 311, 865, 1382, 1772
SspI AATATT 1 cut(s) 1525
SspMI CTAG 7 cut(s) 852, 987, 1140, 2004, 2184, 2547, 2607
SstI GAGCTC 3 cut(s) 362, 458, 984
StuI AGGCCT 1 cut(s) 1084
StyD4I CCNGG 3 cut(s) 934, 1027, 1642
StyI CCWWGG 2 cut(s) 1986, 2086
TaaI ACNGT 9 cut(s) 207, 547, 1172, 1556, 1676, 1794, 2384, 2399, 2524
TaiI ACGT 3 cut(s) 1293, 1688, 2020
TaqII GACCGA 1 cut(s) 1931
TasI AATT 9 cut(s) 368, 1350, 1368, 1656, 1853, 1905, 1959, 1991, 2586
TatI WGTACW 1 cut(s) 1551
TauI GCSGC 2 cut(s) 868, 1775
TfiI GAWTC 4 cut(s) 177, 320, 566, 2554
Tru1I TTAA 9 cut(s) 1127, 1527, 1890, 1980, 2046, 2346, 2585, 2589, 2632
Tru9I TTAA 9 cut(s) 1127, 1527, 1890, 1980, 2046, 2346, 2585, 2589, 2632
TscAI CASTG 3 cut(s) 117, 706, 2418
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 7 cut(s) 5, 8, 32, 188, 229, 1204, 2221
Tsp45I GTSAC 1 cut(s) 547
TspRI CASTG 3 cut(s) 117, 706, 2418
Tth111I GACNNNGTC 1 cut(s) 2318
VpaK11BI GGWCC 3 cut(s) 125, 1646, 1914
VspI ATTAAT 2 cut(s) 2585, 2589
XagI CCTNNNNNAGG 1 cut(s) 1574
XapI RAATTY 2 cut(s) 1905, 1991
XbaI TCTAGA 1 cut(s) 986
XceI RCATGY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 2 cut(s) 1035, 1112
XmnI GAANNNNTTC 2 cut(s) 695, 2267
XspI CTAG 7 cut(s) 852, 987, 1140, 2004, 2184, 2547, 2607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.