Rmu_sc0014629.1_g000001

Extra-large guanine nucleotide-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014629.1
OG0005580
Physical Location & Seq
Forward (+)
1 .. 6238
6238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014629.1_g000001.1.cds

Sequence Viewer

Length: 2635 bp
tatggctgctgcggttatgaggaagctgagtgctgctttggcttcaaaaggtgctgatattgatgtcgagagttacgaagatttgagcgcggagttggaatactcgtttgcagtggagtaccaaggtcctccggtgggttatgaaatcccacatgcgtttccgattgacattcaccgaatcccgactgctgcaccggtttcttcaccgtctctgttgcacaatctgtcactgccgatcatccagcccattgcgaagtccggtcagagcaagaaggccagagtagagtctaaacttggggatgaagtaaaggcggttcttgattcaggtgaagagaagggtgtgtgtagtgaagttgggagctctggtgaattgggttgtggaaagttggagggtgaggtggagttgggtgaggatggtggagaaggtgattggggatcaacagagtctgggttgagctcaagaagtgtttcctctgaggttttctctggcagagaggaagctggtgtggatgagattgttcctcaccatgtgaaaaggccatcgactgtgacttttcgcgatccggattcgaatgacatagttgtgcaggaagaggagttcaatgcctctgatgtggatgagagtttgcaagtgaggccgagggtggagaggaatgggaagaaggggtcgtgctatcgatgtgggaaagggaacaagttcactgagaaggaggtgtgcattgtttgtggtgcaaagtattgctttgattgtgtgctgagggcaatgggatcaatgccggaaggaaggaaatgtgttacttgcattcgttttgggattgatgagtcgaggcgagggagtattggcaagtcttctagtatgctcaggcggcttcttactaaggcgcaggtggaagtggtcatgaaagctgagatatcatgccaagcaaatcaactgccaggcaatcgtgttttcgtgaacgacgagcctcttacacaagaagagctctctagattgcaaggttgcagaaaccctccaaaaaatctaaaaccgggatcttattggtatgataatgtatctggtttttggggaaagattgggcataggccttgccaagttataagcccccagctcaatgttgggggccatttaaaggaaaatgctagtaatggggacacaaaaataataataaacaatcgagtgatcaccaaattagaggtcttcatgctgcagatggcaggagtgccatgtgaaggaaaaatcaactactgggtgagtgcagatggatcctatcaggaagagggaatgaataacgtcaagggaaagatatgggacacgaaaataaagctggtttgtactatcttctctttaccaattccttctgaatctacaaatcctcctcctgcggaagaagacggaaactttattgaagagaaagtgctcgacaaacttgttttagttgggtatcataaatctggctcaagcactatatttaaacaggcaaagcttttgtatgatgttcctttttctgaagatgagcgccaaaatattaagttcatgatccaaagcagattgtacagttatcttggtatactgcttgaggggcgtgaatggtttgaagaagaaagtttggttgaaaacagaaaaggagagcttcaagatgaacctggtccttcagtaattacaagccagctggacagtaaaacaacgtataccattggcccaagactgaaatcttttgcagattggctactaaagaatatgatatcaggtactttggaagccatatttcctgcggctactcgtgaatatgcaccgtttgttgaggagttgtggaatgatccagctattcaagccacatacaaccggaggaatgaattagaaatgctacccagacaagctacatattttcttaatcgggctgtagaaatttcaaggattgattatgagccctcggacatggacatcttgtacgcagagggaattacctcgtccaacagccttaataccatggaattttcattgactagatcatctgaacgtagcaatctggacactttctatcagcataatcctttgcaaaggtatcaactcattagagtgcatagccttggaggaagttgcaagttgctggagatgtttgaagatgttgatatggttatattttgtgttgctttgacagactacgatgagttttgtgaagatgctagtggggtaaccataaacaagatgatggcaagcaagcagctgtttgaaagcattgcaactcatccggcttttgatcagaaacacttccttctgatactcaacaagtttgatctgcttgaagaaaagattgaccaagtccctctcactcgatgcgaatggtttaatgacttcaatccagcaaccagtcataatcccaacagtagtaataataacagtaataaccctacattggctcaccgagcctttcagtatattgcaatgaagttcaaaagattatttcatactcaaacagatagaaagttgtttgtatccctagttactgcattagagcctgatactgtggatgaagctctcagatatgctagtgagattctaagatgtgaggaagaggtacccagattaattaatgagttctcttcggctagcattgaagccagttctacagtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009898 GO:0009987 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200
Pfam Domains
Protein Families
G-alpha

Protein Analysis

877

Amino Acids

98.01

Weight (kDa)

5.08

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
AarI CACCTGC 1 cut(s) 875
Acc36I ACCTGC 1 cut(s) 875
Acc65I GGTACC 1 cut(s) 2576
AccB1I GGYRCC 1 cut(s) 2576
AccI GTMKAC 2 cut(s) 1568, 1689
AccII CGCG 2 cut(s) 90, 559
AccIII TCCGGA 1 cut(s) 563
AciI CCGC 6 cut(s) 12, 90, 312, 866, 1383, 1773
AclWI GGATC 8 cut(s) 443, 555, 776, 1040, 1258, 1271, 1532, 1812
AcsI RAATTY 2 cut(s) 1906, 1992
AcuI CTGAAG 2 cut(s) 1528, 1636
AdeI CACNNNGTG 1 cut(s) 530
AfaI GTAC 6 cut(s) 120, 1334, 1554, 1751, 1950, 2578
AfiI CCNNNNNNNGG 4 cut(s) 135, 1130, 1230, 2414
AgeI ACCGGT 1 cut(s) 194
AjnI CCWGG 2 cut(s) 935, 1643
AloI GAACNNNNNNTCC 2 cut(s) 582, 614
Alw21I GWGCWC 4 cut(s) 363, 459, 985, 1420
Alw26I GTCTC 1 cut(s) 214
AlwI GGATC 8 cut(s) 443, 555, 776, 1040, 1258, 1271, 1532, 1812
AlwNI CAGNNNCTG 1 cut(s) 447
Aor13HI TCCGGA 1 cut(s) 563
AoxI GGCC 6 cut(s) 274, 537, 636, 1083, 1121, 1697
ApeKI GCWGC 6 cut(s) 6, 9, 33, 189, 1205, 2222
ApoI RAATTY 2 cut(s) 1906, 1992
ArsI GACNNNNNNTTYG 2 cut(s) 1180, 1212
AseI ATTAAT 2 cut(s) 2586, 2590
AsiGI ACCGGT 1 cut(s) 194
Asp700I GAANNNNTTC 3 cut(s) 467, 696, 2268
Asp718I GGTACC 1 cut(s) 2576
AspLEI GCGC 3 cut(s) 90, 884, 1519
AspS9I GGNCC 4 cut(s) 126, 1121, 1647, 1698
AsuC2I CCSGG 1 cut(s) 1030
AsuII TTCGAA 1 cut(s) 570
AsuNHI GCTAGC 1 cut(s) 2607
AvaII GGWCC 2 cut(s) 126, 1647
BamHI GGATCC 1 cut(s) 1263
BanI GGYRCC 1 cut(s) 2576
BanII GRGCYC 4 cut(s) 363, 459, 985, 1930
BauI CACGAG 1 cut(s) 1780
BbsI GAAGAC 3 cut(s) 841, 1190, 1396
Bbv12I GWGCWC 4 cut(s) 363, 459, 985, 1420
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 4 cut(s) 20, 176, 1192, 2234
BccI CCATC 5 cut(s) 408, 548, 1205, 1254, 2204
BciT130I CCWGG 2 cut(s) 937, 1645
BciVI GTATCC 1 cut(s) 2504
BclI TGATCA 2 cut(s) 1180, 2258
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 1 cut(s) 214
BfaI CTAG 8 cut(s) 853, 988, 1141, 2005, 2185, 2499, 2548, 2608
BfmI CTRYAG 3 cut(s) 1206, 1900, 2626
BfoI RGCGCY 1 cut(s) 1520
BfuAI ACCTGC 1 cut(s) 875
BfuI GTATCC 1 cut(s) 2504
BisI GCNGC 8 cut(s) 7, 10, 34, 190, 867, 1206, 1774, 2223
BlsI GCNGC 8 cut(s) 8, 11, 35, 191, 868, 1207, 1775, 2224
Bme1390I CCNGG 3 cut(s) 937, 1030, 1645
Bme18I GGWCC 2 cut(s) 126, 1647
BmgT120I GGNCC 4 cut(s) 126, 1121, 1647, 1698
BmiI GGNNCC 3 cut(s) 1122, 1265, 2578
BmrFI CCNGG 3 cut(s) 937, 1030, 1645
BmrI ACTGGG 1 cut(s) 1256
BmsI GCATC 2 cut(s) 2171, 2325
BmtI GCTAGC 1 cut(s) 2611
BmuI ACTGGG 1 cut(s) 1256
BpiI GAAGAC 3 cut(s) 841, 1190, 1396
BplI GAGNNNNNCTC 2 cut(s) 468, 500
BpmI CTGGAG 1 cut(s) 2130
Bpu10I CCTNAGC 2 cut(s) 756, 861
Bpu14I TTCGAA 1 cut(s) 570
BpuEI CTTGAG 3 cut(s) 443, 1442, 1596
BpuMI CCSGG 1 cut(s) 1030
Bsa29I ATCGAT 1 cut(s) 677
BsaJI CCNNGG 5 cut(s) 122, 639, 1930, 1987, 2087
BsaWI WCCGGW 5 cut(s) 131, 194, 258, 563, 1843
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 1358, 1388
Bsc4I CCNNNNNNNGG 4 cut(s) 135, 1130, 1230, 2414
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 3 cut(s) 1251, 2368, 2620
Bse3DI GCAATG 4 cut(s) 247, 769, 2236, 2449
BseAI TCCGGA 1 cut(s) 563
BseBI CCWGG 2 cut(s) 937, 1645
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 5 cut(s) 122, 639, 1930, 1987, 2087
BseGI GGATG 7 cut(s) 238, 305, 419, 515, 623, 2246, 2534
BseLI CCNNNNNNNGG 4 cut(s) 135, 1130, 1230, 2414
BseMI GCAATG 4 cut(s) 247, 769, 2236, 2449
BseMII CTCAG 7 cut(s) 18, 466, 694, 747, 875, 898, 2552
BseNI ACTGG 3 cut(s) 1251, 2368, 2620
BseRI GAGGAG 3 cut(s) 609, 1366, 1818
BseXI GCAGC 4 cut(s) 20, 176, 1192, 2234
BseYI CCCAGC 1 cut(s) 1105
BsgI GTGCAG 3 cut(s) 175, 606, 1276
Bsh1236I CGCG 2 cut(s) 90, 559
BshFI GGCC 6 cut(s) 276, 539, 638, 1085, 1123, 1699
BshNI GGYRCC 1 cut(s) 2576
BshTI ACCGGT 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 4 cut(s) 363, 459, 985, 1420
BsiSI CCGG 8 cut(s) 132, 195, 259, 564, 777, 1029, 1844, 2250
BslFI GGGAC 3 cut(s) 1164, 1323, 2307
BslI CCNNNNNNNGG 4 cut(s) 135, 1130, 1230, 2414
BsmAI GTCTC 1 cut(s) 214
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 3 cut(s) 1164, 1323, 2307
BsmI GAATGC 1 cut(s) 802
BsnI GGCC 6 cut(s) 276, 539, 638, 1085, 1123, 1699
Bsp119I TTCGAA 1 cut(s) 570
Bsp1286I GDGCHC 5 cut(s) 363, 459, 985, 1420, 1930
Bsp13I TCCGGA 1 cut(s) 563
Bsp1407I TGTACA 1 cut(s) 1552
Bsp19I CCATGG 1 cut(s) 1987
Bsp68I TCGCGA 1 cut(s) 559
BspACI CCGC 6 cut(s) 12, 90, 312, 866, 1383, 1773
BspANI GGCC 6 cut(s) 276, 539, 638, 1085, 1123, 1699
BspCNI CTCAG 7 cut(s) 19, 467, 695, 748, 874, 899, 2551
BspDI ATCGAT 1 cut(s) 677
BspEI TCCGGA 1 cut(s) 563
BspFNI CGCG 2 cut(s) 90, 559
BspHI TCATGA 2 cut(s) 898, 1534
BspLI GGNNCC 3 cut(s) 1122, 1265, 2578
BspMAI CTGCAG 1 cut(s) 1210
BspMI ACCTGC 1 cut(s) 875
BspOI GCTAGC 1 cut(s) 2611
BspPI GGATC 8 cut(s) 443, 555, 776, 1040, 1258, 1271, 1532, 1812
BspQI GCTCTTC 1 cut(s) 973
BspT104I TTCGAA 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 2576
BsrDI GCAATG 4 cut(s) 247, 769, 2236, 2449
BsrFI RCCGGY 1 cut(s) 194
BsrGI TGTACA 1 cut(s) 1552
BsrI ACTGG 3 cut(s) 1251, 2368, 2620
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 5 cut(s) 122, 639, 1930, 1987, 2087
BssNAI GTATAC 2 cut(s) 1569, 1690
BssSI CACGAG 1 cut(s) 1780
BssT1I CCWWGG 3 cut(s) 122, 1987, 2087
Bst1107I GTATAC 2 cut(s) 1569, 1690
Bst2BI CACGAG 1 cut(s) 1780
Bst2UI CCWGG 2 cut(s) 937, 1645
Bst4CI ACNGT 9 cut(s) 208, 548, 1557, 1677, 1795, 2385, 2400, 2525, 2630
Bst6I CTCTTC 7 cut(s) 325, 586, 973, 1270, 1402, 2566, 2606
BstAUI TGTACA 1 cut(s) 1552
BstBI TTCGAA 1 cut(s) 570
BstC8I GCNNGC 4 cut(s) 1668, 2216, 2220, 2609
BstDEI CTNAG 9 cut(s) 27, 475, 703, 756, 861, 877, 907, 2538, 2559
BstDSI CCRYGG 1 cut(s) 1987
BstEII GGTNACC 1 cut(s) 2192
BstF5I GGATG 7 cut(s) 238, 305, 419, 515, 623, 2246, 2534
BstFNI CGCG 2 cut(s) 90, 559
BstH2I RGCGCY 1 cut(s) 1520
BstHHI GCGC 3 cut(s) 90, 884, 1519
BstMAI GTCTC 1 cut(s) 214
BstMWI GCNNNNNNNGC 6 cut(s) 39, 635, 866, 1580, 1830, 2424
BstNI CCWGG 2 cut(s) 937, 1645
BstNSI RCATGY 1 cut(s) 156
BstPI GGTNACC 1 cut(s) 2192
BstSCI CCNGG 3 cut(s) 935, 1028, 1643
BstSFI CTRYAG 3 cut(s) 1206, 1900, 2626
BstUI CGCG 2 cut(s) 90, 559
BstV1I GCAGC 4 cut(s) 20, 176, 1192, 2234
BstV2I GAAGAC 3 cut(s) 841, 1190, 1396
BstX2I RGATCY 2 cut(s) 1032, 1263
BstYI RGATCY 2 cut(s) 1032, 1263
BstZ17I GTATAC 2 cut(s) 1569, 1690
Bsu15I ATCGAT 1 cut(s) 677
BsuI GTATCC 1 cut(s) 2504
BsuRI GGCC 6 cut(s) 276, 539, 638, 1085, 1123, 1699
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 1987
BtsCI GGATG 7 cut(s) 238, 305, 419, 515, 623, 2246, 2534
BtsI GCAGTG 2 cut(s) 118, 228
BtsIMutI CAGTG 3 cut(s) 118, 228, 700
BtuMI TCGCGA 1 cut(s) 559
BveI ACCTGC 1 cut(s) 875
Cac8I GCNNGC 4 cut(s) 1668, 2216, 2220, 2609
CaiI CAGNNNCTG 1 cut(s) 447
CciI TCATGA 2 cut(s) 898, 1534
CfoI GCGC 3 cut(s) 90, 884, 1519
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 4 cut(s) 126, 1121, 1647, 1698
ClaI ATCGAT 1 cut(s) 677
CsiI ACCWGGT 1 cut(s) 1643
Csp6I GTAC 6 cut(s) 119, 1333, 1553, 1750, 1949, 2577
CspAI ACCGGT 1 cut(s) 194
CviAII CATG 9 cut(s) 153, 528, 899, 916, 1202, 1225, 1535, 1937, 1988
CviQI GTAC 6 cut(s) 119, 1333, 1553, 1750, 1949, 2577
DdeI CTNAG 9 cut(s) 27, 475, 703, 756, 861, 877, 907, 2538, 2559
DraI TTTAAA 2 cut(s) 1129, 1472
DraIII CACNNNGTG 1 cut(s) 530
Eam1104I CTCTTC 7 cut(s) 325, 586, 973, 1270, 1402, 2566, 2606
EarI CTCTTC 7 cut(s) 325, 586, 973, 1270, 1402, 2566, 2606
Ecl136II GAGCTC 3 cut(s) 361, 457, 983
Eco130I CCWWGG 3 cut(s) 122, 1987, 2087
Eco147I AGGCCT 1 cut(s) 1085
Eco24I GRGCYC 4 cut(s) 363, 459, 985, 1930
Eco32I GATATC 2 cut(s) 913, 1744
Eco47I GGWCC 2 cut(s) 126, 1647
Eco53kI GAGCTC 3 cut(s) 361, 457, 983
Eco57I CTGAAG 2 cut(s) 1528, 1636
Eco91I GGTNACC 1 cut(s) 2192
EcoICRI GAGCTC 3 cut(s) 361, 457, 983
EcoO109I RGGNCCY 1 cut(s) 126
EcoO65I GGTNACC 1 cut(s) 2192
EcoRII CCWGG 2 cut(s) 935, 1643
EcoRV GATATC 2 cut(s) 913, 1744
EcoT14I CCWWGG 3 cut(s) 122, 1987, 2087
EcoT38I GRGCYC 4 cut(s) 363, 459, 985, 1930
ErhI CCWWGG 3 cut(s) 122, 1987, 2087
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 9 cut(s) 156, 531, 902, 919, 1205, 1228, 1538, 1940, 1991
FalI AAGNNNNNCTT 4 cut(s) 726, 758, 1615, 1647
FaqI GGGAC 3 cut(s) 1164, 1323, 2307
FatI CATG 9 cut(s) 152, 527, 898, 915, 1201, 1224, 1534, 1936, 1987
FbaI TGATCA 2 cut(s) 1180, 2258
FblI GTMKAC 2 cut(s) 1568, 1689
Fnu4HI GCNGC 8 cut(s) 7, 10, 34, 190, 867, 1206, 1774, 2223
FokI GGATG 7 cut(s) 225, 312, 426, 522, 630, 2233, 2541
FriOI GRGCYC 4 cut(s) 363, 459, 985, 1930
Fsp4HI GCNGC 8 cut(s) 7, 10, 34, 190, 867, 1206, 1774, 2223
FspBI CTAG 8 cut(s) 853, 988, 1141, 2005, 2185, 2499, 2548, 2608
GlaI GCGC 3 cut(s) 89, 883, 1518
GluI GCNGC 8 cut(s) 7, 10, 34, 190, 867, 1206, 1774, 2223
GsaI CCCAGC 1 cut(s) 1109
GsuI CTGGAG 1 cut(s) 2130
HaeII RGCGCY 1 cut(s) 1520
HaeIII GGCC 6 cut(s) 276, 539, 638, 1085, 1123, 1699
HapII CCGG 8 cut(s) 132, 195, 259, 564, 777, 1029, 1844, 2250
HhaI GCGC 3 cut(s) 90, 884, 1519
Hin1II CATG 9 cut(s) 156, 531, 902, 919, 1205, 1228, 1538, 1940, 1991
Hin6I GCGC 3 cut(s) 88, 882, 1517
HinP1I GCGC 3 cut(s) 88, 882, 1517
HindIII AAGCTT 1 cut(s) 1482
HinfI GANTC 8 cut(s) 178, 285, 321, 444, 567, 822, 1362, 2555
HpaII CCGG 8 cut(s) 132, 195, 259, 564, 777, 1029, 1844, 2250
Hpy166II GTNNAC 4 cut(s) 700, 956, 1569, 1690
Hpy8I GTNNAC 4 cut(s) 700, 956, 1569, 1690
Hpy99I CGWCG 1 cut(s) 963
HpyCH4III ACNGT 9 cut(s) 208, 548, 1557, 1677, 1795, 2385, 2400, 2525, 2630
HpyCH4IV ACGT 3 cut(s) 1291, 1686, 2018
HpyF10VI GCNNNNNNNGC 6 cut(s) 39, 635, 866, 1580, 1830, 2424
HpyF3I CTNAG 9 cut(s) 27, 475, 703, 756, 861, 877, 907, 2538, 2559
HpySE526I ACGT 3 cut(s) 1291, 1686, 2018
Hsp92II CATG 9 cut(s) 156, 531, 902, 919, 1205, 1228, 1538, 1940, 1991
HspAI GCGC 3 cut(s) 88, 882, 1517
Kpn2I TCCGGA 1 cut(s) 563
KpnI GGTACC 1 cut(s) 2580
Ksp22I TGATCA 2 cut(s) 1180, 2258
LguI GCTCTTC 1 cut(s) 973
LmnI GCTCC 1 cut(s) 358
Lsp1109I GCAGC 4 cut(s) 20, 176, 1192, 2234
LweI GCATC 2 cut(s) 2171, 2325
MabI ACCWGGT 1 cut(s) 1643
MaeI CTAG 8 cut(s) 853, 988, 1141, 2005, 2185, 2499, 2548, 2608
MaeII ACGT 3 cut(s) 1291, 1686, 2018
MaeIII GTNAC 6 cut(s) 72, 226, 548, 794, 2192, 2501
MflI RGATCY 2 cut(s) 1032, 1263
MhlI GDGCHC 5 cut(s) 363, 459, 985, 1420, 1930
MluCI AATT 9 cut(s) 369, 1188, 1351, 1657, 1854, 1906, 1960, 1992, 2587
MlyI GAGTC 3 cut(s) 294, 453, 831
MmeI TCCRAC 3 cut(s) 76, 367, 1996
MroI TCCGGA 1 cut(s) 563
MroXI GAANNNNTTC 3 cut(s) 467, 696, 2268
MseI TTAA 9 cut(s) 1128, 1471, 1528, 1891, 1981, 2347, 2586, 2590, 2633
MslI CAYNNNNRTG 2 cut(s) 582, 2076
MspA1I CMGCKG 2 cut(s) 1670, 2225
MspI CCGG 8 cut(s) 132, 195, 259, 564, 777, 1029, 1844, 2250
MspR9I CCNGG 3 cut(s) 937, 1030, 1645
Mva1269I GAATGC 1 cut(s) 802
MvaI CCWGG 2 cut(s) 937, 1645
MvnI CGCG 2 cut(s) 90, 559
MwoI GCNNNNNNNGC 6 cut(s) 39, 635, 866, 1580, 1830, 2424
NciI CCSGG 1 cut(s) 1030
NcoI CCATGG 1 cut(s) 1987
NheI GCTAGC 1 cut(s) 2607
NlaIII CATG 9 cut(s) 156, 531, 902, 919, 1205, 1228, 1538, 1940, 1991
NlaIV GGNNCC 3 cut(s) 1122, 1265, 2578
NmeAIII GCCGAG 1 cut(s) 664
NmuCI GTSAC 2 cut(s) 226, 548
NruI TCGCGA 1 cut(s) 559
NspI RCATGY 1 cut(s) 156
NspV TTCGAA 1 cut(s) 570
PacI TTAATTAA 1 cut(s) 2590
PagI TCATGA 2 cut(s) 898, 1534
PaqCI CACCTGC 1 cut(s) 875
PceI AGGCCT 1 cut(s) 1085
PciSI GCTCTTC 1 cut(s) 973
PcsI WCGNNNNNNNCGW 2 cut(s) 73, 958
PctI GAATGC 1 cut(s) 802
PdmI GAANNNNTTC 3 cut(s) 467, 696, 2268
PfeI GAWTC 5 cut(s) 178, 321, 567, 1362, 2555
PflFI GACNNNGTC 1 cut(s) 2319
PinAI ACCGGT 1 cut(s) 194
PkrI GCNGC 8 cut(s) 8, 11, 35, 191, 868, 1207, 1775, 2224
PleI GAGTC 3 cut(s) 293, 452, 830
PpsI GAGTC 3 cut(s) 293, 452, 830
PpuMI RGGWCCY 1 cut(s) 126
PshBI ATTAAT 2 cut(s) 2586, 2590
PsiI TTATAA 1 cut(s) 1098
Psp124BI GAGCTC 3 cut(s) 363, 459, 985
Psp5II RGGWCCY 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 935, 1643
PspEI GGTNACC 1 cut(s) 2192
PspFI CCCAGC 1 cut(s) 1105
PspGI CCWGG 2 cut(s) 935, 1643
PspN4I GGNNCC 3 cut(s) 1122, 1265, 2578
PspPI GGNCC 4 cut(s) 126, 1121, 1647, 1698
PspPPI RGGWCCY 1 cut(s) 126
PsrI GAACNNNNNNTAC 2 cut(s) 298, 330
PstI CTGCAG 1 cut(s) 1210
PstNI CAGNNNCTG 1 cut(s) 447
PsuI RGATCY 2 cut(s) 1032, 1263
PsyI GACNNNGTC 1 cut(s) 2319
PvuII CAGCTG 2 cut(s) 1670, 2225
RruI TCGCGA 1 cut(s) 559
RsaI GTAC 6 cut(s) 120, 1334, 1554, 1751, 1950, 2578
RsaNI GTAC 6 cut(s) 119, 1333, 1553, 1750, 1949, 2577
RseI CAYNNNNRTG 2 cut(s) 582, 2076
SacI GAGCTC 3 cut(s) 363, 459, 985
SapI GCTCTTC 1 cut(s) 973
SaqAI TTAA 9 cut(s) 1128, 1471, 1528, 1891, 1981, 2347, 2586, 2590, 2633
SatI GCNGC 8 cut(s) 7, 10, 34, 190, 867, 1206, 1774, 2223
Sau96I GGNCC 4 cut(s) 126, 1121, 1647, 1698
SchI GAGTC 3 cut(s) 294, 453, 831
ScrFI CCNGG 3 cut(s) 937, 1030, 1645
SduI GDGCHC 5 cut(s) 363, 459, 985, 1420, 1930
SexAI ACCWGGT 1 cut(s) 1643
SfaNI GCATC 2 cut(s) 2171, 2325
SfcI CTRYAG 3 cut(s) 1206, 1900, 2626
SfuI TTCGAA 1 cut(s) 570
SinI GGWCC 2 cut(s) 126, 1647
SmiMI CAYNNNNRTG 2 cut(s) 582, 2076
SmlI CTYRAG 3 cut(s) 458, 1457, 1575
SmoI CTYRAG 3 cut(s) 458, 1457, 1575
Sse9I AATT 9 cut(s) 369, 1188, 1351, 1657, 1854, 1906, 1960, 1992, 2587
SseBI AGGCCT 1 cut(s) 1085
SsiI CCGC 6 cut(s) 12, 90, 312, 866, 1383, 1773
SspI AATATT 1 cut(s) 1526
SspMI CTAG 8 cut(s) 853, 988, 1141, 2005, 2185, 2499, 2548, 2608
SstI GAGCTC 3 cut(s) 363, 459, 985
StuI AGGCCT 1 cut(s) 1085
StyD4I CCNGG 3 cut(s) 935, 1028, 1643
StyI CCWWGG 3 cut(s) 122, 1987, 2087
TaaI ACNGT 9 cut(s) 208, 548, 1557, 1677, 1795, 2385, 2400, 2525, 2630
TaiI ACGT 3 cut(s) 1294, 1689, 2021
TaqI TCGA 8 cut(s) 67, 543, 570, 677, 825, 1175, 1420, 2333
TasI AATT 9 cut(s) 369, 1188, 1351, 1657, 1854, 1906, 1960, 1992, 2587
TatI WGTACW 2 cut(s) 1332, 1552
TauI GCSGC 2 cut(s) 869, 1776
TfiI GAWTC 5 cut(s) 178, 321, 567, 1362, 2555
Tru1I TTAA 9 cut(s) 1128, 1471, 1528, 1891, 1981, 2347, 2586, 2590, 2633
Tru9I TTAA 9 cut(s) 1128, 1471, 1528, 1891, 1981, 2347, 2586, 2590, 2633
TscAI CASTG 3 cut(s) 118, 235, 707
TseFI GTSAC 2 cut(s) 226, 548
TseI GCWGC 6 cut(s) 6, 9, 33, 189, 1205, 2222
Tsp45I GTSAC 2 cut(s) 226, 548
TspGWI ACGGA 1 cut(s) 1408
TspRI CASTG 3 cut(s) 118, 235, 707
Tth111I GACNNNGTC 1 cut(s) 2319
VpaK11BI GGWCC 2 cut(s) 126, 1647
VspI ATTAAT 2 cut(s) 2586, 2590
XapI RAATTY 2 cut(s) 1906, 1992
XbaI TCTAGA 1 cut(s) 987
XceI RCATGY 1 cut(s) 156
XcmI CCANNNNNNNNNTGG 1 cut(s) 1113
XmiI GTMKAC 2 cut(s) 1568, 1689
XmnI GAANNNNTTC 3 cut(s) 467, 696, 2268
XspI CTAG 8 cut(s) 853, 988, 1141, 2005, 2185, 2499, 2548, 2608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.