MD10G1086100.v1.1

Extra-large guanine nucleotide-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005580
Physical Location & Seq
Forward (+)
12647874 .. 12653783
5910 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1086100.v1.1.491

Sequence Viewer

Length: 2730 bp
ATGGCGTATGTGATGAGAAAATTGAGTTCTGCTATGGCCGCTTCTGGTGGGGGCGGCGGCGTCGATCCCGTAGATTTTAGCACGGAGTTGGAGTTTTCGTTTGCCGATGAATACAAGGGTCCTCCGGTAGGTTATGTTATCCCAAATGCAGTTCCGGTTGATATTCACCACATCCCAACTGCTGCTCCGGTTTCTTCAGGCTCTCTCTTGAACAATTCGTCCCTCCCTGTTATTCAACCGATTGCGAAATCTAACAAGAAACCGAAAAAAGAGTCGAAACTTGCAATTGAAGCAAAGTTTGGAAAGGAAATTGTTTCTTCCGATTCAGTAACTCCTGCTGAATTGGAAATTAGCAAAGGCGAAAAGGGTGTGTCGACTTTATCAGATAAAGTCGAAAGCTCTGGGGATTTGGCGTTTTCTGGCAGTCTGTCTGGAAGTTCAAAGGGTGTGCTTGAACTACCTGATGATGGTCAAGAAGCTCAAGGTTTTCAGAATGATATGAGCCCCGGTGATTGGGGTTCATCAGAGTCTGGTTCGAGTTCACAGAGTCTTTCCTCTGAGGTTTTTTCTGGTAGAGAGGAAGCTGATGCGGATGAAATTCCTCACCATGTCAAAAGACCATCAGCTGTGACATTCCGTGATCCTGACTCGAATGACATTGTTCAGGAAGAAGAGTATGATCTTTCTGATGCGGAAGAAGATGTGCAAGTGAGGCCTACAGTTGAGAGAAATGGGAAGAAAGGGTCGTGCTATCGATGTGGGAAAGGAAACAGACTCACGGAGAAGGAGGTTTGCATTGTTTGTGGTGCTAAGTATTGCTATAATTGTGTGCTGAGAGCAATGGGGTCAATGCCGGAAGGAAGAAAATGTATTACTTGCATTAGTTTTGAAATTGACGAGTCAAGGCGAAGGAAGCTGGGCAAGTGTTCTAGGATGCTCAAGCGGCTTCTCACTAAATCGGAAGTTGAACTGATAATGAAAGCCGAGATATCTTGCCAAGCAAATCAACTGCCAGGCAATCTTATTTTTGTGAACGACGAACATCTATCGCAGGAAGAGCTGGTTCGATTGCAAAGTTGCCGAAACCCGCCAAAAAAGTTAAAACCAGGGTGCTATTGGTATGATAAAGTATCTGGTTTTTGGGGAAAGGAAGGGCATAGGCCTTGCCAAATAATTAGCCCTCACTTGAATGTGCGAGGTCACATAAGGCAAAATGCTAGTAACGGGGATACAAATATATTGATAAACGGTCGGGAGATCACTAAGTTAGAGGCCTTCATGCTGAAGTTGGCTGGAGTGCCATGTGAAGGAAATCTTCACTACTGGGTGAGTGCAGATGGGTCGTATCAGGAAGAAGGAATGAAGCAAGTAAAAGGAAAGATATGGGACAAGACTACAATAAAGCTGGTTTGTAACATCTTGTCTTTGCCAATTCCTTCTGATTCTGCAAATCTTTCGACAGAAGAAGTGAACACCCAGAATACCTTTGAAGAGAAAATGCTTAACAAAATTGTTCTAGTTGGACATCATAAATCTGGTACAAGCACTATATTTAAACAGGCCAAGATTTTGTACAATGTTCCTTTCTCTGAAGATGAACGGCAAAGTATTAAGTTGATGATACAGAGCAGATTGTACAGTTATCTCGGTATACTGCTTGAAGGACGTGAGTGGTTTGAAGAAGAAAGTTTGATTGAGAAGAGAAAAGGAAAAGGACACCTTCTGGAGGAACCAGGCCCGTCAGGAAATACAAGCCAGCTTGACGATAAAACGAAATATTCCATTGGTCCAAGACTGAAAGCTTTTTCTGATTGGCTCCTCAAATCTATGGTATCTGGTAATCTGGAAGCCATATTTCCAGCAGCTACTCGTGAATATGCACCATTTGTTGAGGATTTGTGGAAGGATCCTGCTATTCAAGCCACATACGACCGGAGGAATGAAATACAAACGCTGCCCAGACCAGCTGCATATTTTCTTAATCGGGCCGTTGAGATTTCAAGGACAGATTATGAGCCCTCAGACATGGACATCTTGTATGCTGAGGGAATCACCTCATCCAATAGCCTTACGAGCATGGAATTTTCGATTCCCCCATCAGCAGGAAACAGCAATCTGGACCCTCCTTATCAGCATGACCCTTCGTTAAGGTACCAACTAATTAGAGTGCATCAGAGCAGCCTTGGAGGAAACTGCAAGTTGGTCGAGATGTTTGAAGATGTTAATATGGTCGCATTTTGTGTTTCATTGACAGACTACGATGAGTTTTATACAGATGTTAATGGGGTTTCCATAAACAAGATGATGGCAAGTAAGCAGCTATTTGAAAATATTGTTACTCATCGGGCCTTTGACCAAAAAAACTTCCTTCTGATACTCAACAAGTTTGATCTTCTGGAAGAAAAGATTGACGAAGTCCCTCTCACTCGATGCGAATGGTTTAATGATTTCAATCCAGTACCCAGCCAGAATCCCAGCAGTAGCCACAATAACAGCAATAACCCCACGTTGGCTCACCGAGCCTTTCAGTATATTGCAGTGAAGTTCAAAAGGCTCTTTCATTCTCTAACAGATAGGAAGTTGTTTGTTTCACTGGTTACAGCGCTAGAGTCTGATACTGTAGATGAAGCTCTTAGATATGCTAGTGAGATTCTAAAATTGGAAGAAGAGGTACCCAGATTAATTAACGAGTTCTCTTCTGCTAGCATCGAGGCCAGTTCTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0001664 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0003924 GO:0005102 GO:0005488 GO:0005515 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005834 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007186 GO:0007187 GO:0007188 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009898 GO:0009987 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0019897 GO:0019898 GO:0023052 GO:0031234 GO:0031683 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032991 GO:0035639 GO:0036094 GO:0042742 GO:0043167 GO:0043168 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:0097367 GO:0098542 GO:0098552 GO:0098562 GO:0098796 GO:0098797 GO:1901265 GO:1901363 GO:1902494 GO:1905360
KEGG Pathways
Metabolic & Signaling
ko04015 ko04022 ko04024 ko04062 ko04071 ko04261 ko04360 ko04371 ko04540 ko04611 ko04670 ko04713 ko04723 ko04724 ko04725 ko04726 ko04727 ko04728 ko04730 ko04914 ko04915 ko04916 ko04921 ko04923 ko04924 ko04926 ko04934 ko04971 ko05012 ko05030 ko05032 ko05034 ko05133 ko05142 ko05145 ko05200 map04015 map04022 map04024 map04062 map04071 map04261 map04360 map04371 map04540 map04611 map04670 map04713 map04723 map04724 map04725 map04726 map04727 map04728 map04730 map04914 map04915 map04916 map04921 map04923 map04924 map04926 map04934 map04971 map05012 map05030 map05032 map05034 map05133 map05142 map05145 map05200
Pfam Domains
Protein Families
G-alpha

Protein Analysis

910

Amino Acids

101.19

Weight (kDa)

5.41

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-alpha PF00503 490 - 883 3.2e-66 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 2151, 2671
AccB1I GGYRCC 2 cut(s) 2151, 2671
AccI GTMKAC 2 cut(s) 374, 1651
AciI CCGC 7 cut(s) 39, 54, 57, 590, 692, 943, 1088
AclWI GGATC 4 cut(s) 59, 635, 1901, 1914
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 597, 2081
AcuI CTGAAG 3 cut(s) 180, 1304, 1611
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 6 cut(s) 1540, 1574, 1637, 2153, 2460, 2673
AfeI AGCGCT 1 cut(s) 2604
AfiI CCNNNNNNNGG 7 cut(s) 128, 467, 513, 1307, 1726, 2102, 2509
AjiI CACGTC 1 cut(s) 1667
AjnI CCWGG 3 cut(s) 1012, 1105, 1732
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AloI GAACNNNNNNTCC 2 cut(s) 203, 235
AlwI GGATC 4 cut(s) 59, 635, 1901, 1914
AlwNI CAGNNNCTG 1 cut(s) 530
Aor51HI AGCGCT 1 cut(s) 2604
AoxI GGCC 9 cut(s) 36, 713, 1160, 1272, 1560, 1735, 1986, 2346, 2712
ApeKI GCWGC 6 cut(s) 182, 1862, 1954, 1967, 2178, 2317
ApoI RAATTY 2 cut(s) 597, 2081
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AseI ATTAAT 1 cut(s) 2681
Asp718I GGTACC 2 cut(s) 2151, 2671
AspLEI GCGC 1 cut(s) 2605
AspS9I GGNCC 6 cut(s) 119, 1736, 1787, 1986, 2119, 2346
AsuC2I CCSGG 1 cut(s) 507
AsuHPI GGTGA 6 cut(s) 158, 521, 596, 1339, 2044, 2507
AsuNHI GCTAGC 1 cut(s) 2702
AvaII GGWCC 3 cut(s) 119, 1787, 2119
BamHI GGATCC 1 cut(s) 1906
BanI GGYRCC 2 cut(s) 2151, 2671
BanII GRGCYC 2 cut(s) 506, 2019
BarI GAAGNNNNNNTAC 2 cut(s) 2655, 2687
BauI CACGAG 1 cut(s) 1869
BbvCI CCTCAGC 1 cut(s) 2043
BbvI GCAGC 6 cut(s) 169, 1874, 1941, 1954, 2190, 2329
BccI CCATC 5 cut(s) 461, 628, 1331, 2104, 2299
BceAI ACGGC 2 cut(s) 1616, 1973
BcgI CGANNNNNNTGC 6 cut(s) 1323, 1357, 1437, 1471, 2185, 2219
BciT130I CCWGG 3 cut(s) 1014, 1107, 1734
BciVI GTATCC 1 cut(s) 1222
BcnI CCSGG 1 cut(s) 507
BfaI CTAG 6 cut(s) 930, 1218, 1517, 2606, 2643, 2703
BfmI CTRYAG 2 cut(s) 717, 2619
BfoI RGCGCY 1 cut(s) 2606
BfuI GTATCC 1 cut(s) 1222
Bme1390I CCNGG 4 cut(s) 507, 1014, 1107, 1734
Bme18I GGWCC 3 cut(s) 119, 1787, 2119
BmgBI CACGTC 1 cut(s) 1667
BmgT120I GGNCC 6 cut(s) 119, 1736, 1787, 1986, 2119, 2346
BmiI GGNNCC 7 cut(s) 120, 1731, 1817, 1908, 2121, 2153, 2673
BmrFI CCNGG 4 cut(s) 507, 1014, 1107, 1734
BmrI ACTGGG 1 cut(s) 1333
BmsI GCATC 6 cut(s) 577, 679, 924, 2179, 2420, 2715
BmtI GCTAGC 1 cut(s) 2706
BmuI ACTGGG 1 cut(s) 1333
BpmI CTGGAG 2 cut(s) 1314, 1745
Bpu10I CCTNAGC 1 cut(s) 2043
BpuEI CTTGAG 2 cut(s) 465, 923
BpuMI CCSGG 1 cut(s) 507
Bsa29I ATCGAT 1 cut(s) 754
BsaBI GATNNNNATC 1 cut(s) 2451
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 505, 1106, 2182
BsaWI WCCGGW 4 cut(s) 124, 154, 187, 1932
BsaXI ACNNNNNCTCC 6 cut(s) 77, 83, 107, 113, 169, 199
Bsc4I CCNNNNNNNGG 7 cut(s) 128, 467, 513, 1307, 1726, 2102, 2509
Bse1I ACTGG 4 cut(s) 1328, 2456, 2598, 2715
Bse3DI GCAATG 1 cut(s) 846
Bse8I GATNNNNATC 1 cut(s) 2451
BseBI CCWGG 3 cut(s) 1014, 1107, 1734
BseCI ATCGAT 1 cut(s) 754
BseDI CCNNGG 3 cut(s) 505, 1106, 2182
BseGI GGATG 4 cut(s) 171, 598, 939, 2057
BseJI GATNNNNATC 1 cut(s) 2451
BseLI CCNNNNNNNGG 7 cut(s) 128, 467, 513, 1307, 1726, 2102, 2509
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 4 cut(s) 549, 824, 2034, 2034
BseNI ACTGG 4 cut(s) 1328, 2456, 2598, 2715
BseRI GAGGAG 1 cut(s) 1808
BseXI GCAGC 6 cut(s) 169, 1874, 1941, 1954, 2190, 2329
BseYI CCCAGC 3 cut(s) 916, 2462, 2474
BsgI GTGCAG 1 cut(s) 1353
Bsh1285I CGRYCG 2 cut(s) 1252, 1933
BshFI GGCC 9 cut(s) 38, 715, 1162, 1274, 1562, 1737, 1988, 2348, 2714
BshNI GGYRCC 2 cut(s) 2151, 2671
BshVI ATCGAT 1 cut(s) 754
BsiEI CGRYCG 2 cut(s) 1252, 1933
BsiSI CCGG 6 cut(s) 125, 155, 188, 507, 854, 1933
BslFI GGGAC 3 cut(s) 205, 1400, 2402
BslI CCNNNNNNNGG 7 cut(s) 128, 467, 513, 1307, 1726, 2102, 2509
BsmFI GGGAC 3 cut(s) 205, 1400, 2402
BsnI GGCC 9 cut(s) 38, 715, 1162, 1274, 1562, 1737, 1988, 2348, 2714
Bsp1286I GDGCHC 2 cut(s) 506, 2019
Bsp1407I TGTACA 2 cut(s) 1572, 1635
Bsp143I GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
BspACI CCGC 7 cut(s) 39, 54, 57, 590, 692, 943, 1088
BspANI GGCC 9 cut(s) 38, 715, 1162, 1274, 1562, 1737, 1988, 2348, 2714
BspCNI CTCAG 4 cut(s) 550, 825, 2033, 2035
BspDI ATCGAT 1 cut(s) 754
BspLI GGNNCC 7 cut(s) 120, 1731, 1817, 1908, 2121, 2153, 2673
BspOI GCTAGC 1 cut(s) 2706
BspPI GGATC 4 cut(s) 59, 635, 1901, 1914
BspQI GCTCTTC 1 cut(s) 1050
BspT107I GGYRCC 2 cut(s) 2151, 2671
BsrDI GCAATG 1 cut(s) 846
BsrGI TGTACA 2 cut(s) 1572, 1635
BsrI ACTGG 4 cut(s) 1328, 2456, 2598, 2715
BssECI CCNNGG 3 cut(s) 505, 1106, 2182
BssMI GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
BssNAI GTATAC 1 cut(s) 1652
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 1869
BssT1I CCWWGG 1 cut(s) 2182
Bst1107I GTATAC 1 cut(s) 1652
Bst2BI CACGAG 1 cut(s) 1869
Bst2UI CCWGG 3 cut(s) 1014, 1107, 1734
Bst4CI ACNGT 4 cut(s) 721, 1250, 1640, 2620
Bst6I CTCTTC 6 cut(s) 666, 1050, 1485, 1694, 2661, 2701
BstACI GRCGYC 1 cut(s) 60
BstAUI TGTACA 2 cut(s) 1572, 1635
BstC8I GCNNGC 2 cut(s) 1757, 2704
BstDEI CTNAG 7 cut(s) 558, 810, 833, 1263, 2020, 2043, 2633
BstENI CCTNNNNNAGG 2 cut(s) 126, 1724
BstF5I GGATG 4 cut(s) 171, 598, 939, 2057
BstH2I RGCGCY 1 cut(s) 2606
BstHHI GCGC 1 cut(s) 2605
BstKTI GATC 6 cut(s) 67, 643, 682, 1260, 1909, 2392
BstMBI GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
BstMCI CGRYCG 2 cut(s) 1252, 1933
BstMWI GCNNNNNNNGC 9 cut(s) 38, 290, 712, 913, 943, 1057, 1919, 2073, 2519
BstNI CCWGG 3 cut(s) 1014, 1107, 1734
BstSCI CCNGG 4 cut(s) 505, 1012, 1105, 1732
BstSFI CTRYAG 2 cut(s) 717, 2619
BstV1I GCAGC 6 cut(s) 169, 1874, 1941, 1954, 2190, 2329
BstX2I RGATCY 1 cut(s) 1906
BstYI RGATCY 1 cut(s) 1906
BstZ17I GTATAC 1 cut(s) 1652
Bsu15I ATCGAT 1 cut(s) 754
BsuI GTATCC 1 cut(s) 1222
BsuRI GGCC 9 cut(s) 38, 715, 1162, 1274, 1562, 1737, 1988, 2348, 2714
BsuTUI ATCGAT 1 cut(s) 754
BtrI CACGTC 1 cut(s) 1667
BtsCI GGATG 4 cut(s) 171, 598, 939, 2057
BtsI GCAGTG 1 cut(s) 2544
BtsIMutI CAGTG 2 cut(s) 2544, 2591
Cac8I GCNNGC 2 cut(s) 1757, 2704
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 1 cut(s) 2605
Cfr13I GGNCC 6 cut(s) 119, 1736, 1787, 1986, 2119, 2346
ClaI ATCGAT 1 cut(s) 754
CseI GACGC 1 cut(s) 49
Csp6I GTAC 6 cut(s) 1539, 1573, 1636, 2152, 2459, 2672
CviAII CATG 6 cut(s) 608, 1279, 1302, 2026, 2077, 2135
CviQI GTAC 6 cut(s) 1539, 1573, 1636, 2152, 2459, 2672
DdeI CTNAG 7 cut(s) 558, 810, 833, 1263, 2020, 2043, 2633
DpnI GATC 6 cut(s) 66, 642, 681, 1259, 1908, 2391
DpnII GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
DraI TTTAAA 1 cut(s) 1555
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 6 cut(s) 666, 1050, 1485, 1694, 2661, 2701
EarI CTCTTC 6 cut(s) 666, 1050, 1485, 1694, 2661, 2701
Eco130I CCWWGG 1 cut(s) 2182
Eco147I AGGCCT 3 cut(s) 715, 1162, 1274
Eco24I GRGCYC 2 cut(s) 506, 2019
Eco32I GATATC 1 cut(s) 990
Eco47I GGWCC 3 cut(s) 119, 1787, 2119
Eco47III AGCGCT 1 cut(s) 2604
Eco57I CTGAAG 3 cut(s) 180, 1304, 1611
EcoNI CCTNNNNNAGG 2 cut(s) 126, 1724
EcoO109I RGGNCCY 1 cut(s) 119
EcoRII CCWGG 3 cut(s) 1012, 1105, 1732
EcoRV GATATC 1 cut(s) 990
EcoT14I CCWWGG 1 cut(s) 2182
EcoT38I GRGCYC 2 cut(s) 506, 2019
ErhI CCWWGG 1 cut(s) 2182
FaeI CATG 6 cut(s) 611, 1282, 1305, 2029, 2080, 2138
FalI AAGNNNNNCTT 4 cut(s) 1299, 1331, 1704, 1736
FaqI GGGAC 3 cut(s) 205, 1400, 2402
FatI CATG 6 cut(s) 607, 1278, 1301, 2025, 2076, 2134
FauI CCCGC 1 cut(s) 1095
FblI GTMKAC 2 cut(s) 374, 1651
FokI GGATG 4 cut(s) 158, 605, 946, 2044
FriOI GRGCYC 2 cut(s) 506, 2019
FspBI CTAG 6 cut(s) 930, 1218, 1517, 2606, 2643, 2703
GlaI GCGC 1 cut(s) 2604
GsaI CCCAGC 3 cut(s) 920, 2466, 2478
GsuI CTGGAG 2 cut(s) 1314, 1745
HaeII RGCGCY 1 cut(s) 2606
HaeIII GGCC 9 cut(s) 38, 715, 1162, 1274, 1562, 1737, 1988, 2348, 2714
HapII CCGG 6 cut(s) 125, 155, 188, 507, 854, 1933
HgaI GACGC 1 cut(s) 49
HhaI GCGC 1 cut(s) 2605
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 6 cut(s) 611, 1282, 1305, 2029, 2080, 2138
Hin6I GCGC 1 cut(s) 2603
HinP1I GCGC 1 cut(s) 2603
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 1800
HpaII CCGG 6 cut(s) 125, 155, 188, 507, 854, 1933
HphI GGTGA 6 cut(s) 158, 521, 596, 1339, 2044, 2507
Hpy166II GTNNAC 5 cut(s) 375, 542, 1033, 1471, 1652
Hpy8I GTNNAC 5 cut(s) 375, 542, 1033, 1471, 1652
Hpy99I CGWCG 2 cut(s) 65, 1040
HpyCH4III ACNGT 4 cut(s) 721, 1250, 1640, 2620
HpyCH4IV ACGT 2 cut(s) 1666, 2507
HpyF10VI GCNNNNNNNGC 9 cut(s) 38, 290, 712, 913, 943, 1057, 1919, 2073, 2519
HpyF3I CTNAG 7 cut(s) 558, 810, 833, 1263, 2020, 2043, 2633
HpySE526I ACGT 2 cut(s) 1666, 2507
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 6 cut(s) 611, 1282, 1305, 2029, 2080, 2138
HspAI GCGC 1 cut(s) 2603
KpnI GGTACC 2 cut(s) 2155, 2675
Kzo9I GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
LguI GCTCTTC 1 cut(s) 1050
LmnI GCTCC 2 cut(s) 190, 1821
Lsp1109I GCAGC 6 cut(s) 169, 1874, 1941, 1954, 2190, 2329
LweI GCATC 6 cut(s) 577, 679, 924, 2179, 2420, 2715
MaeI CTAG 6 cut(s) 930, 1218, 1517, 2606, 2643, 2703
MaeII ACGT 2 cut(s) 1666, 2507
MaeIII GTNAC 7 cut(s) 328, 628, 1199, 1220, 1412, 2335, 2596
MalI GATC 6 cut(s) 66, 642, 681, 1259, 1908, 2391
MboI GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 1 cut(s) 1906
MhlI GDGCHC 2 cut(s) 506, 2019
MlyI GAGTC 7 cut(s) 281, 536, 556, 641, 768, 908, 2618
MmeI TCCRAC 2 cut(s) 69, 1501
MslI CAYNNNNRTG 1 cut(s) 1188
MspA1I CMGCKG 2 cut(s) 626, 1967
MspI CCGG 6 cut(s) 125, 155, 188, 507, 854, 1933
MspR9I CCNGG 4 cut(s) 507, 1014, 1107, 1734
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 3 cut(s) 1014, 1107, 1734
MwoI GCNNNNNNNGC 9 cut(s) 38, 290, 712, 913, 943, 1057, 1919, 2073, 2519
NciI CCSGG 1 cut(s) 507
NdeII GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
NheI GCTAGC 1 cut(s) 2702
NlaIII CATG 6 cut(s) 611, 1282, 1305, 2029, 2080, 2138
NlaIV GGNNCC 7 cut(s) 120, 1731, 1817, 1908, 2121, 2153, 2673
NmeAIII GCCGAG 1 cut(s) 1009
NmuCI GTSAC 2 cut(s) 628, 1199
PacI TTAATTAA 1 cut(s) 2685
PceI AGGCCT 3 cut(s) 715, 1162, 1274
PciSI GCTCTTC 1 cut(s) 1050
PfeI GAWTC 6 cut(s) 323, 1442, 2049, 2089, 2470, 2650
PflFI GACNNNGTC 1 cut(s) 2414
PleI GAGTC 7 cut(s) 280, 535, 555, 641, 768, 907, 2617
PpsI GAGTC 7 cut(s) 280, 535, 555, 641, 768, 907, 2617
PpuMI RGGWCCY 1 cut(s) 119
PshBI ATTAAT 1 cut(s) 2681
Psp5II RGGWCCY 1 cut(s) 119
Psp6I CCWGG 3 cut(s) 1012, 1105, 1732
PspFI CCCAGC 3 cut(s) 916, 2462, 2474
PspGI CCWGG 3 cut(s) 1012, 1105, 1732
PspN4I GGNNCC 7 cut(s) 120, 1731, 1817, 1908, 2121, 2153, 2673
PspPI GGNCC 6 cut(s) 119, 1736, 1787, 1986, 2119, 2346
PspPPI RGGWCCY 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 1 cut(s) 1906
PsyI GACNNNGTC 1 cut(s) 2414
PvuII CAGCTG 2 cut(s) 626, 1967
RsaI GTAC 6 cut(s) 1540, 1574, 1637, 2153, 2460, 2673
RsaNI GTAC 6 cut(s) 1539, 1573, 1636, 2152, 2459, 2672
RseI CAYNNNNRTG 1 cut(s) 1188
SalI GTCGAC 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 1050
Sau3AI GATC 6 cut(s) 64, 640, 679, 1257, 1906, 2389
Sau96I GGNCC 6 cut(s) 119, 1736, 1787, 1986, 2119, 2346
SchI GAGTC 7 cut(s) 281, 536, 556, 641, 768, 908, 2618
ScrFI CCNGG 4 cut(s) 507, 1014, 1107, 1734
SduI GDGCHC 2 cut(s) 506, 2019
SfaNI GCATC 6 cut(s) 577, 679, 924, 2179, 2420, 2715
SfcI CTRYAG 2 cut(s) 717, 2619
SinI GGWCC 3 cut(s) 119, 1787, 2119
SmiMI CAYNNNNRTG 1 cut(s) 1188
SmlI CTYRAG 2 cut(s) 480, 938
SmoI CTYRAG 2 cut(s) 480, 938
SseBI AGGCCT 3 cut(s) 715, 1162, 1274
SsiI CCGC 7 cut(s) 39, 54, 57, 590, 692, 943, 1088
SspI AATATT 2 cut(s) 1778, 2332
SspMI CTAG 6 cut(s) 930, 1218, 1517, 2606, 2643, 2703
StuI AGGCCT 3 cut(s) 715, 1162, 1274
StyD4I CCNGG 4 cut(s) 505, 1012, 1105, 1732
StyI CCWWGG 1 cut(s) 2182
TaaI ACNGT 4 cut(s) 721, 1250, 1640, 2620
TaiI ACGT 2 cut(s) 1669, 2510
TatI WGTACW 2 cut(s) 1572, 1635
TauI GCSGC 4 cut(s) 41, 57, 60, 946
TfiI GAWTC 6 cut(s) 323, 1442, 2049, 2089, 2470, 2650
TscAI CASTG 2 cut(s) 2544, 2598
TseFI GTSAC 2 cut(s) 628, 1199
TseI GCWGC 6 cut(s) 182, 1862, 1954, 1967, 2178, 2317
Tsp45I GTSAC 2 cut(s) 628, 1199
TspGWI ACGGA 3 cut(s) 98, 626, 794
TspRI CASTG 2 cut(s) 2544, 2598
Tth111I GACNNNGTC 1 cut(s) 2414
VpaK11BI GGWCC 3 cut(s) 119, 1787, 2119
VspI ATTAAT 1 cut(s) 2681
XagI CCTNNNNNAGG 2 cut(s) 126, 1724
XapI RAATTY 2 cut(s) 597, 2081
XcmI CCANNNNNNNNNTGG 1 cut(s) 1113
XmiI GTMKAC 2 cut(s) 374, 1651
XspI CTAG 6 cut(s) 930, 1218, 1517, 2606, 2643, 2703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.