MD05G1079300.v1.1

Zinc-binding alcohol dehydrogenase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007410
Physical Location & Seq
Reverse (-)
16674594 .. 16683483
8890 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1079300.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGGAGCTAAAAGCAGGCCTCTCAGCTCTAGTCACCGGCGGAGCCTCCGGAATCGGAAAAGCTCTGAGCTTAGCTCTGGCAGCGAAGGGAATATTTGTGACAGTCGTCGATTTCTCTGAAGAAAAGGGGAAGGAAGTTGCTTCCCTTGTTCAGAAGGAGAATGCCAAGTTCCACTCCAATTTAGGTTTCCCGTCTGCCGTGTTTGTAAAATGCGATGTTTCAAATACAGGAGATATAACTGCTGCGTTCGAGAAGCATTTAGCAACATTTGGAGGATTGGATATCTGTATAAACAATGCAGGCATTGGTACTTTCATACCATTTGATAAGGATCAAACCGATGGTACTCATACCTGGAGGAAAACAATTAATGTGAATCTGGTGGCAGTTATTGATTGCACTCGCCTTGCGATTAAAACCATGGAAGCTTTGAAAAAGCCTGGCGTAATTATCAATATGGGGTCTTCCTCAGGTCTTTACCCATTCTATACTGATCCAATCTACTCTGGCTCCAAAGGTGGGGTTGTTCAATTTACGAGATCTCTTGTTCCATACAAACTTAGAGGGATTCGCATCAATGTGCTTTGTCCTGAATTTGTTGAAACTGAGATGGGCTCAAAGGTGGGTTCTAAATTCATTAGTCTAATGGACGGCTATGTGTCCATGGAAATGGTGGTAAAAGGCGCTTTTGAGCTTATTACTGATGAGAGTAAAGCTGGCTCATGCCTATGGATTACAAATCGAAGAGGCATGGAATATTGGCCAACTCCCGCAGAAGAAGCAAAATACTTGGTTCGCCGTTCAAGTTTGAGGAAAAGAGCTGTGGTAGAAGCTCCTTTCAATTTTCAACTTCCAGAAAGTTTTGAGAAATTAGTTGTTCACACCTTGAGTCACAATTTTCGTAATGCTACCTCCATGGTGCGCGCACCATTGAGACTACCCATTGAACCGCACAGCGTTCTTGTGAAAATCATATATGCTGGTGTCAATGCTAGTGATGTAAACTACAGTTCAGGACGTTATTTCAGTGGCAAAACCAAAGATATTGAGTCCCGTCTTCCATTTGATTGCGGTTTTGAGGCTGTGGGGATAATTGCAGCAGTAGGGGACTCTGTTACTAACTTGCAAGTTGGTACTCCAGCTGCAATCATGACTTTTGGAAGTTATGCAGAATTCACGTTGGTACCTTCAAAACACATCCTTCCTGTGGAAAGACCAGATCCAGAAGTTGTTGCTATGCTTACTTCAGGACTGACTGCATCAATCGCTCTAGAAAAGGCGGGACAAATGGAATCTGGGAAAGTAGTTCTTGTCACTGCTGCTGCTGGAGGGACTGGGCAATTTGCAGTCCAGCTTGCAAAACTTGCCGGAAATACTGTGGTTGCAACTTGTGGAGGTGCAGAAAAGGCAAAGCATTTGAAAGAATTGGGGGTTGATCGAGTCATTGACTATAAAGCTGAAGATGTCAAAACTGTTCTAAGGAAGGAGTATCCCAAAGGTGTCGATATTATCTATGAGTCTGTTGGTGGTGACATGTTTGATTTGTGCTTGAATGCTCTTGCGGTCTATGGGCGACTCATTGTAATCGGAATGATTTCTCAGTATCAAGGAGAGCATGGGTGGAAGCCATCAAACTATCCCGGAATTTGCGAAAAGATTTTAGCGAAAAGCCAAACTGTGGCTGGTTTCTTCCTAGTGCAATACAGTCACCTTTGGCAAGAACATCTGGATAAACTAGTTTATCTTTTCTCCAAGGGAAAGCTGAAGGTTTCGTTAGACCCAAAACAGTTTCTGGGCGTCCGTTCGGCTGCAGATGCTGTGGAGTATCTCCATTCCGGTAAAAGCGTAGGGAAGGTTGTCGTTTGCATCGATCCATCCTTTAGGCAACAAACGTCACGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
ADH_N ADH_zinc_N adh_short

Protein Analysis

635

Amino Acids

68.65

Weight (kDa)

8.74

Isoelectric Point (pI)

27.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 9 - 209 2.7e-43 short chain dehydrogenase
adh_short_C2 PF13561 15 - 210 2.1e-31 Enoyl-(Acyl carrier protein) reductase
ADH_N PF08240 317 - 404 1e-11 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 444 - 578 1.6e-25 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 476 - 621 7.4e-20 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1183
AccB1I GGYRCC 1 cut(s) 1183
AccB7I CCANNNNNTGG 1 cut(s) 1678
AccII CGCG 1 cut(s) 924
AccIII TCCGGA 1 cut(s) 47
AciI CCGC 6 cut(s) 39, 771, 950, 1071, 1280, 1562
AclWI GGATC 4 cut(s) 339, 488, 1214, 1865
AcoI YGGCCR 1 cut(s) 761
AcsI RAATTY 4 cut(s) 593, 632, 1172, 1644
AcuI CTGAAG 4 cut(s) 138, 1230, 1479, 1784
AcyI GRCGYC 1 cut(s) 1797
AfaI GTAC 4 cut(s) 310, 346, 1135, 1185
AfiI CCNNNNNNNGG 3 cut(s) 519, 1207, 1678
AflIII ACRYGT 1 cut(s) 1533
AhlI ACTAGT 1 cut(s) 1735
AjnI CCWGG 2 cut(s) 353, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
Alw26I GTCTC 1 cut(s) 928
AlwI GGATC 4 cut(s) 339, 488, 1214, 1865
AlwNI CAGNNNCTG 2 cut(s) 1792, 1817
Aor13HI TCCGGA 1 cut(s) 47
AoxI GGCC 2 cut(s) 16, 761
ApeKI GCWGC 7 cut(s) 80, 242, 1097, 1142, 1319, 1322, 1808
ApoI RAATTY 4 cut(s) 593, 632, 1172, 1644
AseI ATTAAT 1 cut(s) 369
Asp700I GAANNNNTTC 1 cut(s) 1594
Asp718I GGTACC 1 cut(s) 1183
AspLEI GCGC 3 cut(s) 686, 924, 926
AsuC2I CCSGG 1 cut(s) 1641
AsuHPI GGTGA 3 cut(s) 25, 1541, 1700
AxyI CCTNAGG 1 cut(s) 469
BaeI ACNNNNGTAYC 2 cut(s) 1167, 1200
BalI TGGCCA 1 cut(s) 763
BanI GGYRCC 1 cut(s) 1183
BanII GRGCYC 1 cut(s) 617
BbsI GAAGAC 2 cut(s) 456, 1049
BbvI GCAGC 7 cut(s) 92, 229, 1109, 1129, 1306, 1309, 1795
BccI CCATC 4 cut(s) 335, 604, 1636, 1882
BceAI ACGGC 3 cut(s) 182, 667, 783
BciT130I CCWGG 2 cut(s) 355, 441
BciVI GTATCC 1 cut(s) 1500
BcnI CCSGG 1 cut(s) 1641
BcoDI GTCTC 1 cut(s) 928
BcuI ACTAGT 1 cut(s) 1735
BfaI CTAG 5 cut(s) 29, 993, 1271, 1694, 1736
BfmI CTRYAG 2 cut(s) 1006, 1809
BfoI RGCGCY 1 cut(s) 687
BfuI GTATCC 1 cut(s) 1500
BglII AGATCT 1 cut(s) 539
BisI GCNGC 7 cut(s) 81, 243, 1098, 1143, 1320, 1323, 1809
BlpI GCTNAGC 1 cut(s) 70
BlsI GCNGC 7 cut(s) 82, 244, 1099, 1144, 1321, 1324, 1810
Bme1390I CCNGG 3 cut(s) 355, 441, 1641
BmiI GGNNCC 3 cut(s) 43, 511, 1185
BmrFI CCNGG 3 cut(s) 355, 441, 1641
BmrI ACTGGG 1 cut(s) 1344
BmsI GCATC 4 cut(s) 582, 1268, 1804, 1875
BmuI ACTGGG 1 cut(s) 1344
BoxI GACNNNNGTC 1 cut(s) 104
BpiI GAAGAC 2 cut(s) 456, 1049
BplI GAGNNNNNCTC 4 cut(s) 58, 90, 599, 631
BpmI CTGGAG 3 cut(s) 376, 1122, 1347
Bpu1102I GCTNAGC 1 cut(s) 70
BpuEI CTTGAG 1 cut(s) 907
BpuMI CCSGG 1 cut(s) 1641
Bsa29I ATCGAT 1 cut(s) 1869
BsaBI GATNNNNATC 2 cut(s) 330, 572
BsaHI GRCGYC 1 cut(s) 1797
BsaJI CCNNGG 4 cut(s) 420, 663, 915, 1752
BsaWI WCCGGW 2 cut(s) 47, 1835
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 519, 1207, 1678
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 1339
Bse21I CCTNAGG 1 cut(s) 469
Bse8I GATNNNNATC 2 cut(s) 330, 572
BseAI TCCGGA 1 cut(s) 47
BseBI CCWGG 2 cut(s) 355, 441
BseCI ATCGAT 1 cut(s) 1869
BseDI CCNNGG 4 cut(s) 420, 663, 915, 1752
BseGI GGATG 2 cut(s) 1197, 1874
BseJI GATNNNNATC 2 cut(s) 330, 572
BseLI CCNNNNNNNGG 3 cut(s) 519, 1207, 1678
BseMII CTCAG 5 cut(s) 36, 56, 483, 597, 1613
BseNI ACTGG 1 cut(s) 1339
BsePI GCGCGC 1 cut(s) 922
BseXI GCAGC 7 cut(s) 92, 229, 1109, 1129, 1306, 1309, 1795
BsgI GTGCAG 1 cut(s) 1419
Bsh1236I CGCG 1 cut(s) 924
BshFI GGCC 2 cut(s) 18, 763
BshNI GGYRCC 1 cut(s) 1183
BshVI ATCGAT 1 cut(s) 1869
BsiSI CCGG 5 cut(s) 36, 48, 1368, 1641, 1836
BslFI GGGAC 4 cut(s) 1036, 1121, 1296, 1345
BslI CCNNNNNNNGG 3 cut(s) 519, 1207, 1678
BsmAI GTCTC 1 cut(s) 928
BsmFI GGGAC 4 cut(s) 1036, 1121, 1296, 1345
BsmI GAATGC 2 cut(s) 166, 1558
BsnI GGCC 2 cut(s) 18, 763
Bsp1286I GDGCHC 1 cut(s) 617
Bsp13I TCCGGA 1 cut(s) 47
Bsp143I GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
Bsp1720I GCTNAGC 1 cut(s) 70
Bsp19I CCATGG 3 cut(s) 420, 663, 915
BspACI CCGC 6 cut(s) 39, 771, 950, 1071, 1280, 1562
BspANI GGCC 2 cut(s) 18, 763
BspCNI CTCAG 5 cut(s) 35, 57, 482, 598, 1612
BspDI ATCGAT 1 cut(s) 1869
BspEI TCCGGA 1 cut(s) 47
BspFNI CGCG 1 cut(s) 924
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 3 cut(s) 43, 511, 1185
BspMAI CTGCAG 1 cut(s) 1813
BspPI GGATC 4 cut(s) 339, 488, 1214, 1865
BspT107I GGYRCC 1 cut(s) 1183
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 1339
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 4 cut(s) 420, 663, 915, 1752
BssHII GCGCGC 1 cut(s) 922
BssMI GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
BssNI GRCGYC 1 cut(s) 1797
BssT1I CCWWGG 4 cut(s) 420, 663, 915, 1752
Bst2UI CCWGG 2 cut(s) 355, 441
Bst4CI ACNGT 7 cut(s) 103, 1010, 1378, 1474, 1678, 1706, 1788
Bst6I CTCTTC 1 cut(s) 739
BstACI GRCGYC 1 cut(s) 1797
BstAPI GCANNNNNTGC 1 cut(s) 1364
BstC8I GCNNGC 5 cut(s) 16, 301, 718, 924, 1356
BstDEI CTNAG 8 cut(s) 22, 65, 70, 469, 560, 606, 1478, 1599
BstDSI CCRYGG 3 cut(s) 420, 663, 915
BstF5I GGATG 2 cut(s) 1197, 1874
BstFNI CGCG 1 cut(s) 924
BstH2I RGCGCY 1 cut(s) 687
BstHHI GCGC 3 cut(s) 686, 924, 926
BstKTI GATC 6 cut(s) 334, 496, 542, 1222, 1438, 1873
BstMAI GTCTC 1 cut(s) 928
BstMBI GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
BstMWI GCNNNNNNNGC 6 cut(s) 80, 779, 1265, 1364, 1406, 1814
BstNI CCWGG 2 cut(s) 355, 441
BstNSI RCATGY 1 cut(s) 1537
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 3 cut(s) 353, 439, 1639
BstSFI CTRYAG 2 cut(s) 1006, 1809
BstUI CGCG 1 cut(s) 924
BstV1I GCAGC 7 cut(s) 92, 229, 1109, 1129, 1306, 1309, 1795
BstV2I GAAGAC 2 cut(s) 456, 1049
BstX2I RGATCY 2 cut(s) 539, 1219
BstXI CCANNNNNNTGG 1 cut(s) 670
BstYI RGATCY 2 cut(s) 539, 1219
Bsu15I ATCGAT 1 cut(s) 1869
Bsu36I CCTNAGG 1 cut(s) 469
BsuI GTATCC 1 cut(s) 1500
BsuRI GGCC 2 cut(s) 18, 763
BsuTUI ATCGAT 1 cut(s) 1869
BtgI CCRYGG 3 cut(s) 420, 663, 915
BtgZI GCGATG 1 cut(s) 228
BtsCI GGATG 2 cut(s) 1197, 1874
BtsI GCAGTG 1 cut(s) 1314
BtsIMutI CAGTG 2 cut(s) 1033, 1314
Cac8I GCNNGC 5 cut(s) 16, 301, 718, 924, 1356
CaiI CAGNNNCTG 2 cut(s) 1792, 1817
CciI TCATGA 1 cut(s) 1149
CfoI GCGC 3 cut(s) 686, 924, 926
Cfr10I RCCGGY 1 cut(s) 35
ClaI ATCGAT 1 cut(s) 1869
CseI GACGC 1 cut(s) 1786
Csp6I GTAC 4 cut(s) 309, 345, 1134, 1184
CviAII CATG 8 cut(s) 421, 664, 723, 751, 916, 1150, 1534, 1616
CviQI GTAC 4 cut(s) 309, 345, 1134, 1184
DdeI CTNAG 8 cut(s) 22, 65, 70, 469, 560, 606, 1478, 1599
DpnI GATC 6 cut(s) 333, 495, 541, 1221, 1437, 1872
DpnII GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
EaeI YGGCCR 1 cut(s) 761
Eam1104I CTCTTC 1 cut(s) 739
EarI CTCTTC 1 cut(s) 739
EciI GGCGGA 1 cut(s) 54
Eco130I CCWWGG 4 cut(s) 420, 663, 915, 1752
Eco147I AGGCCT 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 617
Eco32I GATATC 1 cut(s) 283
Eco57I CTGAAG 4 cut(s) 138, 1230, 1479, 1784
Eco81I CCTNAGG 1 cut(s) 469
EcoRI GAATTC 1 cut(s) 1172
EcoRII CCWGG 2 cut(s) 353, 439
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 4 cut(s) 420, 663, 915, 1752
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 4 cut(s) 420, 663, 915, 1752
FaeI CATG 8 cut(s) 424, 667, 726, 754, 919, 1153, 1537, 1619
FalI AAGNNNNNCTT 2 cut(s) 1293, 1325
FaqI GGGAC 4 cut(s) 1036, 1121, 1296, 1345
FatI CATG 8 cut(s) 420, 663, 722, 750, 915, 1149, 1533, 1615
FauI CCCGC 2 cut(s) 778, 1273
Fnu4HI GCNGC 7 cut(s) 81, 243, 1098, 1143, 1320, 1323, 1809
FokI GGATG 2 cut(s) 1184, 1861
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 7 cut(s) 81, 243, 1098, 1143, 1320, 1323, 1809
FspBI CTAG 5 cut(s) 29, 993, 1271, 1694, 1736
GlaI GCGC 3 cut(s) 685, 923, 925
GluI GCNGC 7 cut(s) 81, 243, 1098, 1143, 1320, 1323, 1809
GsuI CTGGAG 3 cut(s) 376, 1122, 1347
HaeII RGCGCY 1 cut(s) 687
HaeIII GGCC 2 cut(s) 18, 763
HapII CCGG 5 cut(s) 36, 48, 1368, 1641, 1836
HgaI GACGC 1 cut(s) 1786
HhaI GCGC 3 cut(s) 686, 924, 926
Hin1I GRCGYC 1 cut(s) 1797
Hin1II CATG 8 cut(s) 424, 667, 726, 754, 919, 1153, 1537, 1619
Hin6I GCGC 3 cut(s) 684, 922, 924
HinP1I GCGC 3 cut(s) 684, 922, 924
HindIII AAGCTT 1 cut(s) 426
HpaII CCGG 5 cut(s) 36, 48, 1368, 1641, 1836
HphI GGTGA 3 cut(s) 25, 1541, 1700
Hpy166II GTNNAC 2 cut(s) 880, 1003
Hpy188I TCNGA 5 cut(s) 56, 66, 118, 153, 1589
Hpy8I GTNNAC 2 cut(s) 880, 1003
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 8 cut(s) 79, 124, 148, 1197, 1211, 1477, 1759, 1846
HpyCH4III ACNGT 7 cut(s) 103, 1010, 1378, 1474, 1678, 1706, 1788
HpyCH4IV ACGT 3 cut(s) 1018, 1178, 1892
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 779, 1265, 1364, 1406, 1814
HpyF3I CTNAG 8 cut(s) 22, 65, 70, 469, 560, 606, 1478, 1599
HpySE526I ACGT 3 cut(s) 1018, 1178, 1892
Hsp92I GRCGYC 1 cut(s) 1797
Hsp92II CATG 8 cut(s) 424, 667, 726, 754, 919, 1153, 1537, 1619
HspAI GCGC 3 cut(s) 684, 922, 924
Kpn2I TCCGGA 1 cut(s) 47
KpnI GGTACC 1 cut(s) 1187
Kzo9I GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
LmnI GCTCC 4 cut(s) 4, 41, 515, 838
Lsp1109I GCAGC 7 cut(s) 92, 229, 1109, 1129, 1306, 1309, 1795
LweI GCATC 4 cut(s) 582, 1268, 1804, 1875
MaeI CTAG 5 cut(s) 29, 993, 1271, 1694, 1736
MaeII ACGT 3 cut(s) 1018, 1178, 1892
MaeIII GTNAC 8 cut(s) 31, 97, 890, 1114, 1312, 1529, 1706, 1893
MalI GATC 6 cut(s) 333, 495, 541, 1221, 1437, 1872
MboI GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
MboII GAAGA 7 cut(s) 131, 456, 756, 788, 1049, 1472, 1681
MflI RGATCY 2 cut(s) 539, 1219
MhlI GDGCHC 1 cut(s) 617
MlsI TGGCCA 1 cut(s) 763
MluNI TGGCCA 1 cut(s) 763
MlyI GAGTC 6 cut(s) 898, 1058, 1103, 1449, 1526, 1569
Mox20I TGGCCA 1 cut(s) 763
MroI TCCGGA 1 cut(s) 47
MroXI GAANNNNTTC 1 cut(s) 1594
MscI TGGCCA 1 cut(s) 763
MseI TTAA 2 cut(s) 369, 414
MslI CAYNNNNRTG 4 cut(s) 578, 668, 727, 1900
Msp20I TGGCCA 1 cut(s) 763
MspA1I CMGCKG 1 cut(s) 1142
MspI CCGG 5 cut(s) 36, 48, 1368, 1641, 1836
MspR9I CCNGG 3 cut(s) 355, 441, 1641
Mva1269I GAATGC 2 cut(s) 166, 1558
MvaI CCWGG 2 cut(s) 355, 441
MvnI CGCG 1 cut(s) 924
MwoI GCNNNNNNNGC 6 cut(s) 80, 779, 1265, 1364, 1406, 1814
NciI CCSGG 1 cut(s) 1641
NcoI CCATGG 3 cut(s) 420, 663, 915
NdeII GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
NlaIII CATG 8 cut(s) 424, 667, 726, 754, 919, 1153, 1537, 1619
NlaIV GGNNCC 3 cut(s) 43, 511, 1185
NmuCI GTSAC 7 cut(s) 31, 97, 890, 1312, 1529, 1706, 1893
NspI RCATGY 1 cut(s) 1537
PagI TCATGA 1 cut(s) 1149
PauI GCGCGC 1 cut(s) 922
PceI AGGCCT 1 cut(s) 18
PciI ACATGT 1 cut(s) 1533
PcsI WCGNNNNNNNCGW 1 cut(s) 1866
PctI GAATGC 2 cut(s) 166, 1558
PdmI GAANNNNTTC 1 cut(s) 1594
PfeI GAWTC 4 cut(s) 51, 376, 568, 1292
PflMI CCANNNNNTGG 1 cut(s) 1678
PfoI TCCNGGA 1 cut(s) 1639
PkrI GCNGC 7 cut(s) 82, 244, 1099, 1144, 1321, 1324, 1810
PleI GAGTC 6 cut(s) 897, 1057, 1103, 1448, 1525, 1569
PpsI GAGTC 6 cut(s) 897, 1057, 1103, 1448, 1525, 1569
PscI ACATGT 1 cut(s) 1533
PshAI GACNNNNGTC 1 cut(s) 104
PshBI ATTAAT 1 cut(s) 369
Psp6I CCWGG 2 cut(s) 353, 439
PspGI CCWGG 2 cut(s) 353, 439
PspN4I GGNNCC 3 cut(s) 43, 511, 1185
PstI CTGCAG 1 cut(s) 1813
PstNI CAGNNNCTG 2 cut(s) 1792, 1817
PsuI RGATCY 2 cut(s) 539, 1219
PteI GCGCGC 1 cut(s) 922
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 4 cut(s) 310, 346, 1135, 1185
RsaNI GTAC 4 cut(s) 309, 345, 1134, 1184
RseI CAYNNNNRTG 4 cut(s) 578, 668, 727, 1900
SaqAI TTAA 2 cut(s) 369, 414
SatI GCNGC 7 cut(s) 81, 243, 1098, 1143, 1320, 1323, 1809
Sau3AI GATC 6 cut(s) 331, 493, 539, 1219, 1435, 1870
SchI GAGTC 6 cut(s) 898, 1058, 1103, 1449, 1526, 1569
ScrFI CCNGG 3 cut(s) 355, 441, 1641
SduI GDGCHC 1 cut(s) 617
SfaNI GCATC 4 cut(s) 582, 1268, 1804, 1875
SfcI CTRYAG 2 cut(s) 1006, 1809
SgrAI CRCCGGYG 1 cut(s) 35
SmiMI CAYNNNNRTG 4 cut(s) 578, 668, 727, 1900
SmlI CTYRAG 1 cut(s) 886
SmoI CTYRAG 1 cut(s) 886
SpeI ACTAGT 1 cut(s) 1735
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 6 cut(s) 39, 771, 950, 1071, 1280, 1562
SspI AATATT 2 cut(s) 93, 758
SspMI CTAG 5 cut(s) 29, 993, 1271, 1694, 1736
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 3 cut(s) 353, 439, 1639
StyI CCWWGG 4 cut(s) 420, 663, 915, 1752
TaaI ACNGT 7 cut(s) 103, 1010, 1378, 1474, 1678, 1706, 1788
TaiI ACGT 3 cut(s) 1021, 1181, 1895
TaqI TCGA 6 cut(s) 108, 249, 742, 1438, 1503, 1869
TfiI GAWTC 4 cut(s) 51, 376, 568, 1292
Tru1I TTAA 2 cut(s) 369, 414
Tru9I TTAA 2 cut(s) 369, 414
TscAI CASTG 2 cut(s) 1033, 1321
TseFI GTSAC 7 cut(s) 31, 97, 890, 1312, 1529, 1706, 1893
TseI GCWGC 7 cut(s) 80, 242, 1097, 1142, 1319, 1322, 1808
Tsp45I GTSAC 7 cut(s) 31, 97, 890, 1312, 1529, 1706, 1893
TspDTI ATGAA 2 cut(s) 304, 625
TspGWI ACGGA 1 cut(s) 1790
TspRI CASTG 2 cut(s) 1033, 1321
Van91I CCANNNNNTGG 1 cut(s) 1678
VspI ATTAAT 1 cut(s) 369
XapI RAATTY 4 cut(s) 593, 632, 1172, 1644
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 1 cut(s) 1537
XcmI CCANNNNNNNNNTGG 2 cut(s) 670, 1679
XmnI GAANNNNTTC 1 cut(s) 1594
XspI CTAG 5 cut(s) 29, 993, 1271, 1694, 1736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.