Rw6G008140

Zinc-binding alcohol dehydrogenase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0007410
Physical Location & Seq
Forward (+)
13825050 .. 13849445
24396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008140.1

Sequence Viewer

Length: 1494 bp
ATGCAAGCTATGCAAAAGCCTGGTGTAATTATCAATATGGGGTCTGCCTCAGGTCTTTACCCAATGATCAATGATCCAATTTACTCTGGTTCAAAAGGTGGCGTTGTTCTTTTTACTCGATCCCTAGTTCCCTACAAACGTCAAGGGATTCGTATCAATGTGCTTTGTCCAGAATTTGTGGAAACTGAGATGGGCTCACTGATCGATTCCAAACTTATTAATTTAATGGGGGGCTTTGTCCCAATGGAAATGGTGGTGAAAGGTGCTTTTGAGCTTATAACTGATGAGAGTAAAGCTGGTTCATGTTTATGGATTACAAATCGGAGAGGCATGGAATATTGGCCGTCCCCCACAGAACAAAACAAATACATGCTTCGCTCTTCAAGCTCGAGGAAAAGAACTACGGCAAAATCTCCATTCCAAACTCCTTTGAATCTTCAGATTCCACAGAGCTATGAGAAATTAGTGGTTCACACCTTGACTCACAATTTCCGTAATGCTACCCGTATAGTTCGTGCACCTTTGAGGTTACCGATTGGACCACATCATGTTCTTGTGAAAATCATTTATGCTGGCGTAAATGCAAGTGATGTAAATTTCAGCTCAGGGCGTTATTTCACTGGAGCTGACAAAGGCCCCCAGCTTCCATTTGATGCTGGTTTTGAGGCTGTGGGTATAATTGCAGCAGTAGGGGAATCTGTCACTAACTTGGAAATTGGTACTCCTGCTGCAATCATGACTTTTGGAAGTTATGCTGAATTCACAATGGTGCCTTCAAAACACATCCTTCCTGTGGAAAGACCAGATCCAGAAGTTGTCGCTATGCTTACTTCAGGACTAACAGCATCAATTGCTCTAGAAAAGGCGGGTGGACAATTGGAATCTGGAAAAGTAGTTCTTGTTACTGCTGCTGCTGGAGGGACTGGGCAATTTGCAGTCCAGCTTGCAAAACTAGCTGGAAATAAAGTGGTTGCAACTTGTGGAGGTGCAGACAAGGCAAAGCTTTTAAAAGAATTGGGGGTTGATCGAGTCATAGATTATAAAGCTGAAGACATAAAAACCGTTCTGAAGAAAGAGTTTCCAAAAGGTCTTGACATTATCTACGAGTCAGTTGGTGGTCACATGTTCGATTTGTGCTTGAATGCTCTGGCAGTTTATGGGCGACTCATTGTAATTGGAATGATTTCTCAGTATCAAGGAGAGCATGGGTGGAAGCCATCAAACTACCCTGGAATTTGTGAGAAGCTTTTAGCTAAAAGCCAAACTGTGGCTGGGTTCTTCTTAGTACAATATAGTCAGTTCTGGCAAGAACACCTGGATAAACTTGTTCATCTTTTCTCTGAAGGAAAACTGAAGGTTTCCTTAGACCCGAAACAATTTCTTGGTGTCCATTCTGTTCCGGATGCTGTCGAGTATCTTCATTCCGGTAAAAGTGTAGGGAAGGTTGTTGTTTGCATCGATCCATCCTACACGCAACAAACATCGAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05202 map05202
Pfam Domains
Protein Families
ADH_N ADH_zinc_N adh_short

Protein Analysis

497

Amino Acids

54.06

Weight (kDa)

8.95

Isoelectric Point (pI)

31.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 3 - 82 9.7e-09 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 5 - 67 9.1e-13 short chain dehydrogenase
ADH_N PF08240 180 - 266 1.4e-12 Alcohol dehydrogenase GroES-like domain
ADH_zinc_N PF00107 307 - 440 1.7e-25 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 339 - 484 1.9e-19 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 278, 1041
AccB1I GGYRCC 1 cut(s) 769
AccB7I CCANNNNNTGG 1 cut(s) 1267
AccIII TCCGGA 1 cut(s) 1399
AciI CCGC 1 cut(s) 866
AclWI GGATC 4 cut(s) 68, 114, 800, 1454
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 4 cut(s) 173, 595, 758, 1233
AcuI CTGAAG 6 cut(s) 422, 816, 1068, 1088, 1362, 1373
AfaI GTAC 2 cut(s) 721, 1287
AfiI CCNNNNNNNGG 2 cut(s) 793, 1267
AflIII ACRYGT 1 cut(s) 1122
AgsI TTSAA 5 cut(s) 93, 384, 433, 777, 1141
AjnI CCWGG 3 cut(s) 19, 1228, 1314
AleI CACNNNNGTG 1 cut(s) 767
AloI GAACNNNNNNTCC 2 cut(s) 879, 911
Alw21I GWGCWC 1 cut(s) 520
Alw44I GTGCAC 1 cut(s) 516
AlwI GGATC 4 cut(s) 68, 114, 800, 1454
Ama87I CYCGRG 1 cut(s) 388
Aor13HI TCCGGA 1 cut(s) 1399
AoxI GGCC 2 cut(s) 341, 634
ApaLI GTGCAC 1 cut(s) 516
ApeKI GCWGC 4 cut(s) 683, 728, 908, 911
ApoI RAATTY 4 cut(s) 173, 595, 758, 1233
AseI ATTAAT 1 cut(s) 219
Asp700I GAANNNNTTC 1 cut(s) 1183
AspS9I GGNCC 2 cut(s) 539, 635
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 388
AvaII GGWCC 1 cut(s) 539
AxyI CCTNAGG 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 520
BanI GGYRCC 1 cut(s) 769
BanII GRGCYC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 1056
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 4 cut(s) 695, 715, 895, 898
BccI CCATC 3 cut(s) 184, 1225, 1471
BceAI ACGGC 2 cut(s) 328, 420
BciT130I CCWGG 3 cut(s) 21, 1230, 1316
BclI TGATCA 1 cut(s) 66
BfaI CTAG 3 cut(s) 125, 857, 953
BisI GCNGC 4 cut(s) 684, 729, 909, 912
BlsI GCNGC 4 cut(s) 685, 730, 910, 913
Bme1390I CCNGG 3 cut(s) 21, 1230, 1316
Bme18I GGWCC 1 cut(s) 539
BmeT110I CYCGRG 1 cut(s) 388
BmgT120I GGNCC 2 cut(s) 539, 635
BmiI GGNNCC 2 cut(s) 637, 771
BmrFI CCNGG 3 cut(s) 21, 1230, 1316
BmrI ACTGGG 1 cut(s) 933
BmsI GCATC 4 cut(s) 643, 854, 1393, 1464
BmuI ACTGGG 1 cut(s) 933
BpiI GAAGAC 1 cut(s) 1056
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BpmI CTGGAG 2 cut(s) 642, 936
Bpu10I CCTNAGC 1 cut(s) 604
Bsa29I ATCGAT 2 cut(s) 204, 1458
BsaBI GATNNNNATC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 1228
BsaWI WCCGGW 2 cut(s) 1399, 1424
Bsc4I CCNNNNNNNGG 2 cut(s) 793, 1267
Bse1I ACTGG 2 cut(s) 625, 928
Bse21I CCTNAGG 1 cut(s) 49
Bse8I GATNNNNATC 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 1399
BseBI CCWGG 3 cut(s) 21, 1230, 1316
BseCI ATCGAT 2 cut(s) 204, 1458
BseDI CCNNGG 1 cut(s) 1228
BseGI GGATG 3 cut(s) 783, 1408, 1463
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 2 cut(s) 793, 1267
BseMII CTCAG 4 cut(s) 63, 177, 618, 1202
BseNI ACTGG 2 cut(s) 625, 928
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 4 cut(s) 695, 715, 895, 898
BseYI CCCAGC 2 cut(s) 639, 1271
BsgI GTGCAG 1 cut(s) 1008
BshFI GGCC 2 cut(s) 343, 636
BshNI GGYRCC 1 cut(s) 769
BshVI ATCGAT 2 cut(s) 204, 1458
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 2 cut(s) 1400, 1425
BslFI GGGAC 3 cut(s) 224, 331, 934
BslI CCNNNNNNNGG 2 cut(s) 793, 1267
BsmFI GGGAC 3 cut(s) 224, 331, 934
BsmI GAATGC 1 cut(s) 1147
BsnI GGCC 2 cut(s) 343, 636
BsoBI CYCGRG 1 cut(s) 388
Bsp1286I GDGCHC 2 cut(s) 197, 520
Bsp13I TCCGGA 1 cut(s) 1399
Bsp143I GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
BspACI CCGC 1 cut(s) 866
BspANI GGCC 2 cut(s) 343, 636
BspCNI CTCAG 4 cut(s) 62, 178, 617, 1201
BspDI ATCGAT 2 cut(s) 204, 1458
BspEI TCCGGA 1 cut(s) 1399
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 637, 771
BspPI GGATC 4 cut(s) 68, 114, 800, 1454
BspQI GCTCTTC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 769
BsrI ACTGG 2 cut(s) 625, 928
BssECI CCNNGG 1 cut(s) 1228
BssMI GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
Bst2UI CCWGG 3 cut(s) 21, 1230, 1316
Bst4CI ACNGT 2 cut(s) 1063, 1267
Bst6I CTCTTC 1 cut(s) 385
BstAPI GCANNNNNTGC 2 cut(s) 10, 851
BstC8I GCNNGC 3 cut(s) 6, 574, 945
BstDEI CTNAG 6 cut(s) 49, 186, 604, 1188, 1282, 1363
BstEII GGTNACC 1 cut(s) 528
BstF5I GGATG 3 cut(s) 783, 1408, 1463
BstKTI GATC 7 cut(s) 69, 76, 122, 204, 808, 1027, 1462
BstMBI GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
BstMWI GCNNNNNNNGC 5 cut(s) 10, 384, 851, 953, 995
BstNI CCWGG 3 cut(s) 21, 1230, 1316
BstNSI RCATGY 2 cut(s) 373, 1126
BstPI GGTNACC 1 cut(s) 528
BstSCI CCNGG 3 cut(s) 19, 1228, 1314
BstSLI GKGCMC 1 cut(s) 520
BstV1I GCAGC 4 cut(s) 695, 715, 895, 898
BstV2I GAAGAC 1 cut(s) 1056
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
Bsu15I ATCGAT 2 cut(s) 204, 1458
Bsu36I CCTNAGG 1 cut(s) 49
BsuRI GGCC 2 cut(s) 343, 636
BsuTUI ATCGAT 2 cut(s) 204, 1458
BtsCI GGATG 3 cut(s) 783, 1408, 1463
BtsIMutI CAGTG 2 cut(s) 197, 618
Cac8I GCNNGC 3 cut(s) 6, 574, 945
CciI TCATGA 1 cut(s) 735
Cfr13I GGNCC 2 cut(s) 539, 635
ClaI ATCGAT 2 cut(s) 204, 1458
Csp6I GTAC 2 cut(s) 720, 1286
CviAII CATG 7 cut(s) 303, 331, 370, 548, 736, 1123, 1205
CviQI GTAC 2 cut(s) 720, 1286
DdeI CTNAG 6 cut(s) 49, 186, 604, 1188, 1282, 1363
DpnI GATC 7 cut(s) 68, 75, 121, 203, 807, 1026, 1461
DpnII GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
DraI TTTAAA 1 cut(s) 1008
EaeI YGGCCR 1 cut(s) 341
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 197
Eco47I GGWCC 1 cut(s) 539
Eco57I CTGAAG 6 cut(s) 422, 816, 1068, 1088, 1362, 1373
Eco81I CCTNAGG 1 cut(s) 49
Eco88I CYCGRG 1 cut(s) 388
Eco91I GGTNACC 1 cut(s) 528
EcoO109I RGGNCCY 1 cut(s) 635
EcoO65I GGTNACC 1 cut(s) 528
EcoRI GAATTC 1 cut(s) 758
EcoRII CCWGG 3 cut(s) 19, 1228, 1314
EcoT38I GRGCYC 1 cut(s) 197
FaeI CATG 7 cut(s) 306, 334, 373, 551, 739, 1126, 1208
FalI AAGNNNNNCTT 4 cut(s) 882, 914, 1346, 1378
FaqI GGGAC 3 cut(s) 224, 331, 934
FatI CATG 7 cut(s) 302, 330, 369, 547, 735, 1122, 1204
FauI CCCGC 1 cut(s) 859
FbaI TGATCA 1 cut(s) 66
Fnu4HI GCNGC 4 cut(s) 684, 729, 909, 912
FokI GGATG 3 cut(s) 770, 1415, 1450
FriOI GRGCYC 1 cut(s) 197
Fsp4HI GCNGC 4 cut(s) 684, 729, 909, 912
FspBI CTAG 3 cut(s) 125, 857, 953
GluI GCNGC 4 cut(s) 684, 729, 909, 912
GsaI CCCAGC 2 cut(s) 643, 1275
GsuI CTGGAG 2 cut(s) 642, 936
HaeIII GGCC 2 cut(s) 343, 636
HapII CCGG 2 cut(s) 1400, 1425
Hin1II CATG 7 cut(s) 306, 334, 373, 551, 739, 1126, 1208
HindIII AAGCTT 2 cut(s) 1001, 1244
HpaII CCGG 2 cut(s) 1400, 1425
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 3 cut(s) 472, 518, 872
Hpy188I TCNGA 4 cut(s) 324, 441, 1068, 1342
Hpy188III TCNNGA 9 cut(s) 170, 736, 809, 834, 857, 885, 1091, 1400, 1485
Hpy8I GTNNAC 3 cut(s) 472, 518, 872
HpyAV CCTTC 5 cut(s) 783, 797, 1337, 1348, 1435
HpyCH4III ACNGT 2 cut(s) 1063, 1267
HpyCH4IV ACGT 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 384, 851, 953, 995
HpyF3I CTNAG 6 cut(s) 49, 186, 604, 1188, 1282, 1363
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 7 cut(s) 306, 334, 373, 551, 739, 1126, 1208
Kpn2I TCCGGA 1 cut(s) 1399
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
LguI GCTCTTC 1 cut(s) 385
LmnI GCTCC 1 cut(s) 623
Lsp1109I GCAGC 4 cut(s) 695, 715, 895, 898
LweI GCATC 4 cut(s) 643, 854, 1393, 1464
MaeI CTAG 3 cut(s) 125, 857, 953
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 4 cut(s) 528, 700, 901, 1118
MalI GATC 7 cut(s) 68, 75, 121, 203, 807, 1026, 1461
MboI GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
MboII GAAGA 6 cut(s) 372, 428, 1061, 1081, 1270, 1409
MfeI CAATTG 2 cut(s) 849, 875
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 2 cut(s) 197, 520
MlyI GAGTC 4 cut(s) 475, 1038, 1115, 1158
MnlI CCTC 7 cut(s) 58, 320, 384, 519, 658, 911, 977
MroI TCCGGA 1 cut(s) 1399
MroXI GAANNNNTTC 1 cut(s) 1183
MseI TTAA 3 cut(s) 219, 224, 1007
MslI CAYNNNNRTG 2 cut(s) 307, 767
MspI CCGG 2 cut(s) 1400, 1425
MspR9I CCNGG 3 cut(s) 21, 1230, 1316
MunI CAATTG 2 cut(s) 849, 875
Mva1269I GAATGC 1 cut(s) 1147
MvaI CCWGG 3 cut(s) 21, 1230, 1316
MwoI GCNNNNNNNGC 5 cut(s) 10, 384, 851, 953, 995
NdeII GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
NlaIII CATG 7 cut(s) 306, 334, 373, 551, 739, 1126, 1208
NlaIV GGNNCC 2 cut(s) 637, 771
NmuCI GTSAC 2 cut(s) 700, 1118
NspI RCATGY 2 cut(s) 373, 1126
OliI CACNNNNGTG 1 cut(s) 767
PaeR7I CTCGAG 1 cut(s) 388
PagI TCATGA 1 cut(s) 735
PciI ACATGT 1 cut(s) 1122
PciSI GCTCTTC 1 cut(s) 385
PctI GAATGC 1 cut(s) 1147
PdmI GAANNNNTTC 1 cut(s) 1183
PfeI GAWTC 6 cut(s) 148, 206, 433, 442, 695, 881
PflMI CCANNNNNTGG 1 cut(s) 1267
PkrI GCNGC 4 cut(s) 685, 730, 910, 913
PleI GAGTC 4 cut(s) 475, 1037, 1114, 1158
PpsI GAGTC 4 cut(s) 475, 1037, 1114, 1158
PscI ACATGT 1 cut(s) 1122
PshBI ATTAAT 1 cut(s) 219
PsiI TTATAA 2 cut(s) 278, 1041
Psp6I CCWGG 3 cut(s) 19, 1228, 1314
PspEI GGTNACC 1 cut(s) 528
PspFI CCCAGC 2 cut(s) 639, 1271
PspGI CCWGG 3 cut(s) 19, 1228, 1314
PspN4I GGNNCC 2 cut(s) 637, 771
PspPI GGNCC 2 cut(s) 539, 635
PspXI VCTCGAGB 1 cut(s) 388
PsrI GAACNNNNNNTAC 2 cut(s) 283, 315
PsuI RGATCY 1 cut(s) 805
RsaI GTAC 2 cut(s) 721, 1287
RsaNI GTAC 2 cut(s) 720, 1286
RseI CAYNNNNRTG 2 cut(s) 307, 767
SapI GCTCTTC 1 cut(s) 385
SaqAI TTAA 3 cut(s) 219, 224, 1007
SatI GCNGC 4 cut(s) 684, 729, 909, 912
Sau3AI GATC 7 cut(s) 66, 73, 119, 201, 805, 1024, 1459
Sau96I GGNCC 2 cut(s) 539, 635
SchI GAGTC 4 cut(s) 475, 1038, 1115, 1158
ScrFI CCNGG 3 cut(s) 21, 1230, 1316
SduI GDGCHC 2 cut(s) 197, 520
SfaNI GCATC 4 cut(s) 643, 854, 1393, 1464
Sfr274I CTCGAG 1 cut(s) 388
SinI GGWCC 1 cut(s) 539
SlaI CTCGAG 1 cut(s) 388
SmiMI CAYNNNNRTG 2 cut(s) 307, 767
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
SsiI CCGC 1 cut(s) 866
SspI AATATT 1 cut(s) 338
SspMI CTAG 3 cut(s) 125, 857, 953
StyD4I CCNGG 3 cut(s) 19, 1228, 1314
TaaI ACNGT 2 cut(s) 1063, 1267
TaiI ACGT 1 cut(s) 142
TaqI TCGA 8 cut(s) 118, 204, 389, 1027, 1128, 1410, 1458, 1484
TatI WGTACW 1 cut(s) 1285
TfiI GAWTC 6 cut(s) 148, 206, 433, 442, 695, 881
Tru1I TTAA 3 cut(s) 219, 224, 1007
Tru9I TTAA 3 cut(s) 219, 224, 1007
TscAI CASTG 2 cut(s) 204, 625
TseFI GTSAC 2 cut(s) 700, 1118
TseI GCWGC 4 cut(s) 683, 728, 908, 911
Tsp45I GTSAC 2 cut(s) 700, 1118
TspDTI ATGAA 3 cut(s) 291, 1319, 1409
TspGWI ACGGA 1 cut(s) 482
TspRI CASTG 2 cut(s) 204, 625
Van91I CCANNNNNTGG 1 cut(s) 1267
VneI GTGCAC 1 cut(s) 516
VpaK11BI GGWCC 1 cut(s) 539
VspI ATTAAT 1 cut(s) 219
XapI RAATTY 4 cut(s) 173, 595, 758, 1233
XbaI TCTAGA 1 cut(s) 856
XceI RCATGY 2 cut(s) 373, 1126
XcmI CCANNNNNNNNNTGG 1 cut(s) 1268
XhoI CTCGAG 1 cut(s) 388
XmnI GAANNNNTTC 1 cut(s) 1183
XspI CTAG 3 cut(s) 125, 857, 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.