Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0015213

Physical Location & Seq

Genomic Coordinates Reverse (-)

17123850 .. 17126756

Gene Structure 2907 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1081500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGTCACCATCCCTCTCTGTTCTATTCCTATCCCCTCCCCTGTCCTCAGCAGCTCCCTACTGCAGGGCAACAAGGCTGGCAGCTGCCACCACCCCGGTGGCGCCGGTTTCTGCCGAGGTGGAGGCGGCGAGGCTGGAACCTAGAGTGGAGGAGAGAGATGGGTACTGGGTTTTGAAGGAGAAGTTTAGAGGAGGGATTAATGTTCAGGAGAAGGTGAAGATTGAGAGGGAGCCTATGAAGCTTTTCATGGAAGGTGGGATTGAAGATCTTGCCAAGATCTCTTTTGAGGAACTTGACAAGGCTAAGGATACTAAGGATGATATCGATGTCAGGCTCAAGTGGCTTGGGCTTTTCCACAGGAGAAAGCAACATTATGGTAGATTTATGATGAGATTGAAGCTACCAAATGGTGTCACAACAAGTGCACAAACGAGATATCTTGCGAGTGTGATAAGAAAATATGGCAAGGATGGCTGTGCGGATGTCACAACAAGGCAAAATTGGCAAATCCGCGGAGTAGTACTGCCGGATGTGCTGGAAATTTTGAAAGGTCTTGCCGAAGTTGGATTGACGAGTCTGCAGAGTGGCATGGACAATGTGAGAAATCCAGTTGGAAATCCTCTTGCAGGCATTGACCCACTCGAGGTTGTCGATACAAGACCTTACACAAACTTGTTGTCTCAATATATCACTGGGAATTCACTTGGAAATCCAAATGTCACAAACTTGCCAAGAAAGTGGAATGTGGCTGTGGTGGGCACTCATGACCTTTTCGAGCATCCCCACATCAACGATCTTGCTTACATGCCTGCAACAAAGGATGGCAGATTCGGATTCAATTTGCTCGTTGGAGGATTCTTCAGTCCCAAGCGATGCGCAGAGGCAGTTCCCCTTGATGCCTGGGTCTCCGCGGATGATGTGATCCCAGTTTGCAAAGCAATACTAGAGGCCTATAGAGACCTTGGCTTCAGAGGGAACCGACAAAAAACAAGAATGATGTGGTTGATTGATGAACTAGGCATAGAAGGATTTAGGTCAGAGGTTGTGAAAAGAATGCCTTTCGAAGACCTGGTGAGAGCATCTTCCGAAGACCTGGTTAAGAAGCAATGGGAAAGGAGAGATTATATGGGTGTCCATCCGCAGAAACAAGAAGGTTTAAGCTATGTGGGACTTCACATTCCTGTAGGCCGGCTTCAAGCAGATGACATGGATGAGCTAGCTCGTTTAGCCGATGAGTATGGCACAGGGGAACTCCGGCTCACCGTGGAGCAAAACATAATCCTCCCCAATGTAGAGAATTCAAGAATTGAAGCATTACTCAAAGAGCCTCTTTTGCAAAATAAGTATTCCCCCAAACCTTCAATTCTCATGAAAGGATTGGTGGCCTGCACTGGCAACCAGTTCTGTGGGCAAGCAATCATTGAGACAAAAGCAAGGGCCTTGAAGGTGACCGAGGAGGTGGAAAGGCACGTGGCAGTGACTCGACCGGTGAGGATGCATTGGACAGGGTGTCCAAATACCTGTGCACAAGTGCAAGTGGCGGATATTGGTTTCATGGGGTGCATGGCAAGGGATGAGAATGGGAAGCCTTGTGAAGGGGCTGATGTTTTCTTGGGAGGAAGAATAGGGAGTGACTCACATCTGGGAGATGTTTATAAGAAGAGTGTCCCTTGCAAGGACTTGGTGCCCTTGGTTGTTGACCTCTTGATAAGCCACTTTGGAGCTGTCCGTAGGGAGAGGGAAGAAGGGGAAGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0010035 GO:0010167 GO:0016020 GO:0016491 GO:0016661 GO:0016662 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0050421 GO:0050896 GO:0055114 GO:0098809 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00910 ko01120 map00910 map01120

Pfam Domains

Protein Families

NIR_SIR NIR_SIR_ferr

Protein Analysis

586

Amino Acids

65.32

Weight (kDa)

6.64

Isoelectric Point (pI)

40.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIR_SIR_ferr	PF03460	119 - 188	8.5e-17	Nitrite/Sulfite reductase ferredoxin-like half domain
NIR_SIR	PF01077	197 - 353	2.4e-41	Nitrite and sulphite reductase 4Fe-4S domain
NIR_SIR_ferr	PF03460	379 - 443	4.8e-20	Nitrite/Sulfite reductase ferredoxin-like half domain
NIR_SIR	PF01077	460 - 567	3e-10	Nitrite and sulphite reductase 4Fe-4S domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015213)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15620
fragaria_vesca	FvH4_2g05960
malus_domestica	MD05G1081500.v1.1
prunus_persica	Prupe.8G126000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0257431
rosa_laevigata	RLG00000014695
rosa_multiflora	Rmu_sc0000820.1_g000048 Rmu_sc0003301.1_g000002
rosa_roxburghii	Rroxscaffold_7G00208660 Rroxscaffold_7G00208680
rosa_rugosa	Rorug05G0572600
rosa_samantha	Rh6AG090100 Rh6BG081600 Rh6CG078100 Rh6DG073900
rosa_wichuraiana	Rw6G007870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1656
Acc16I	TGCGCA	1 cut(s)	877
AccB1I	GGYRCC	2 cut(s)	100, 1684
AccII	CGCG	2 cut(s)	513, 911
AciI	CCGC	8 cut(s)	125, 479, 511, 513, 909, 911, 1139, 1541
AclWI	GGATC	1 cut(s)	916
AcsI	RAATTY	3 cut(s)	540, 697, 1297
AcuI	CTGAAG	2 cut(s)	844, 952
AcvI	CACGTG	1 cut(s)	1471
AcyI	GRCGYC	1 cut(s)	101
AfaI	GTAC	2 cut(s)	164, 522
AfiI	CCNNNNNNNGG	5 cut(s)	63, 626, 643, 1595, 1675
AgeI	ACCGGT	1 cut(s)	1486
AhdI	GACNNNNNGTC	1 cut(s)	1509
AjnI	CCWGG	3 cut(s)	899, 1068, 1092
AleI	CACNNNNGTG	1 cut(s)	95
AluBI	AGCT	8 cut(s)	53, 83, 241, 400, 1161, 1216, 1220, 1724
AluI	AGCT	8 cut(s)	53, 83, 241, 400, 1161, 1216, 1220, 1724
Alw21I	GWGCWC	2 cut(s)	427, 1528
Alw26I	GTCTC	4 cut(s)	684, 910, 951, 1418
Alw44I	GTGCAC	2 cut(s)	423, 1524
AlwI	GGATC	1 cut(s)	916
Ama87I	CYCGRG	1 cut(s)	641
AoxI	GGCC	4 cut(s)	948, 1186, 1383, 1437
ApaLI	GTGCAC	2 cut(s)	423, 1524
ApeKI	GCWGC	3 cut(s)	50, 80, 83
ApoI	RAATTY	3 cut(s)	540, 697, 1297
ArsI	GACNNNNNNTTYG	2 cut(s)	662, 694
AseI	ATTAAT	1 cut(s)	198
AsiGI	ACCGGT	1 cut(s)	1486
Asp700I	GAANNNNTTC	1 cut(s)	242
AspLEI	GCGC	2 cut(s)	103, 878
AspS9I	GGNCC	1 cut(s)	1437
AsuC2I	CCSGG	1 cut(s)	95
AsuHPI	GGTGA	5 cut(s)	226, 1084, 1252, 1459, 1501
AsuII	TTCGAA	1 cut(s)	1062
AsuNHI	GCTAGC	1 cut(s)	1216
AvaI	CYCGRG	1 cut(s)	641
BaeGI	GKGCMC	4 cut(s)	427, 761, 1528, 1689
BanI	GGYRCC	2 cut(s)	100, 1684
BarI	GAAGNNNNNNTAC	2 cut(s)	1176, 1208
BbrPI	CACGTG	1 cut(s)	1471
BbsI	GAAGAC	2 cut(s)	1071, 1095
Bbv12I	GWGCWC	2 cut(s)	427, 1528
BbvCI	CCTCAGC	1 cut(s)	46
BbvI	GCAGC	3 cut(s)	62, 70, 92
BccI	CCATC	5 cut(s)	16, 152, 464, 815, 1143
BciT130I	CCWGG	3 cut(s)	901, 1070, 1094
BciVI	GTATCC	1 cut(s)	301
BcnI	CCSGG	1 cut(s)	95
BcoDI	GTCTC	4 cut(s)	684, 910, 951, 1418
BfaI	CTAG	4 cut(s)	141, 944, 1016, 1217
BfmI	CTRYAG	4 cut(s)	61, 578, 952, 1182
BfoI	RGCGCY	1 cut(s)	104
BfuI	GTATCC	1 cut(s)	301
BglII	AGATCT	2 cut(s)	265, 276
BisI	GCNGC	4 cut(s)	51, 81, 84, 126
BlsI	GCNGC	4 cut(s)	52, 82, 85, 127
BmcAI	AGTACT	1 cut(s)	522
Bme1390I	CCNGG	4 cut(s)	95, 901, 1070, 1094
BmeRI	GACNNNNNGTC	1 cut(s)	1509
BmeT110I	CYCGRG	1 cut(s)	641
BmgT120I	GGNCC	1 cut(s)	1437
BmiI	GGNNCC	5 cut(s)	102, 138, 231, 977, 1686
BmrFI	CCNGG	4 cut(s)	95, 901, 1070, 1094
BmrI	ACTGGG	3 cut(s)	175, 702, 920
BmsI	GCATC	5 cut(s)	787, 863, 886, 1088, 1485
BmtI	GCTAGC	1 cut(s)	1220
BmuI	ACTGGG	3 cut(s)	175, 702, 920
BpiI	GAAGAC	2 cut(s)	1071, 1095
Bpu10I	CCTNAGC	2 cut(s)	46, 303
Bpu14I	TTCGAA	1 cut(s)	1062
BpuEI	CTTGAG	1 cut(s)	320
BpuMI	CCSGG	1 cut(s)	95
Bsa29I	ATCGAT	1 cut(s)	324
BsaAI	YACGTR	1 cut(s)	1471
BsaHI	GRCGYC	1 cut(s)	101
BsaI	GGTCTC	2 cut(s)	910, 951
BsaJI	CCNNGG	9 cut(s)	93, 114, 511, 900, 909, 961, 1263, 1452, 1689
BsaWI	WCCGGW	1 cut(s)	1486
Bsc4I	CCNNNNNNNGG	5 cut(s)	63, 626, 643, 1595, 1675
Bse118I	RCCGGY	3 cut(s)	103, 1188, 1486
Bse1I	ACTGG	6 cut(s)	170, 608, 697, 926, 1396, 1399
Bse3DI	GCAATG	1 cut(s)	1112
BseBI	CCWGG	3 cut(s)	901, 1070, 1094
BseCI	ATCGAT	1 cut(s)	324
BseDI	CCNNGG	9 cut(s)	93, 114, 511, 900, 909, 961, 1263, 1452, 1689
BseLI	CCNNNNNNNGG	5 cut(s)	63, 626, 643, 1595, 1675
BseMI	GCAATG	1 cut(s)	1112
BseMII	CTCAG	1 cut(s)	60
BseNI	ACTGG	6 cut(s)	170, 608, 697, 926, 1396, 1399
BseRI	GAGGAG	3 cut(s)	164, 204, 1469
BseSI	GKGCMC	4 cut(s)	427, 761, 1528, 1689
BseXI	GCAGC	3 cut(s)	62, 70, 92
BsgI	GTGCAG	1 cut(s)	1372
Bsh1236I	CGCG	2 cut(s)	513, 911
Bsh1285I	CGRYCG	1 cut(s)	1487
BshFI	GGCC	4 cut(s)	950, 1188, 1385, 1439
BshNI	GGYRCC	2 cut(s)	100, 1684
BshTI	ACCGGT	1 cut(s)	1486
BshVI	ATCGAT	1 cut(s)	324
BsiEI	CGRYCG	1 cut(s)	1487
BsiHKAI	GWGCWC	2 cut(s)	427, 1528
BsiHKCI	CYCGRG	1 cut(s)	641
BsiSI	CCGG	6 cut(s)	95, 104, 527, 1189, 1255, 1487
BslFI	GGGAC	3 cut(s)	849, 1182, 1652
BslI	CCNNNNNNNGG	5 cut(s)	63, 626, 643, 1595, 1675
BsmAI	GTCTC	4 cut(s)	684, 910, 951, 1418
BsmFI	GGGAC	3 cut(s)	849, 1182, 1652
BsmI	GAATGC	1 cut(s)	1059
BsnI	GGCC	4 cut(s)	950, 1188, 1385, 1439
Bso31I	GGTCTC	2 cut(s)	910, 951
BsoBI	CYCGRG	1 cut(s)	641
Bsp119I	TTCGAA	1 cut(s)	1062
Bsp1286I	GDGCHC	4 cut(s)	427, 761, 1528, 1689
Bsp143I	GATC	4 cut(s)	265, 276, 793, 921
BspACI	CCGC	8 cut(s)	125, 479, 511, 513, 909, 911, 1139, 1541
BspANI	GGCC	4 cut(s)	950, 1188, 1385, 1439
BspCNI	CTCAG	1 cut(s)	59
BspDI	ATCGAT	1 cut(s)	324
BspFNI	CGCG	2 cut(s)	513, 911
BspHI	TCATGA	2 cut(s)	763, 1368
BspLI	GGNNCC	5 cut(s)	102, 138, 231, 977, 1686
BspMAI	CTGCAG	2 cut(s)	65, 582
BspOI	GCTAGC	1 cut(s)	1220
BspPI	GGATC	1 cut(s)	916
BspT104I	TTCGAA	1 cut(s)	1062
BspT107I	GGYRCC	2 cut(s)	100, 1684
BspTNI	GGTCTC	2 cut(s)	910, 951
BsrDI	GCAATG	1 cut(s)	1112
BsrFI	RCCGGY	3 cut(s)	103, 1188, 1486
BsrI	ACTGG	6 cut(s)	170, 608, 697, 926, 1396, 1399
BssAI	RCCGGY	3 cut(s)	103, 1188, 1486
BssECI	CCNNGG	9 cut(s)	93, 114, 511, 900, 909, 961, 1263, 1452, 1689
BssMI	GATC	4 cut(s)	265, 276, 793, 921
BssNI	GRCGYC	1 cut(s)	101
BssT1I	CCWWGG	2 cut(s)	961, 1689
Bst2UI	CCWGG	3 cut(s)	901, 1070, 1094
Bst4CI	ACNGT	1 cut(s)	1264
Bst6I	CTCTTC	1 cut(s)	1655
BstACI	GRCGYC	1 cut(s)	101
BstBAI	YACGTR	1 cut(s)	1471
BstBI	TTCGAA	1 cut(s)	1062
BstC8I	GCNNGC	7 cut(s)	78, 628, 810, 1190, 1218, 1387, 1413
BstDEI	CTNAG	3 cut(s)	46, 303, 312
BstDSI	CCRYGG	3 cut(s)	511, 909, 1263
BstEII	GGTNACC	1 cut(s)	1447
BstENI	CCTNNNNNAGG	3 cut(s)	61, 624, 1593
BstFNI	CGCG	2 cut(s)	513, 911
BstH2I	RGCGCY	1 cut(s)	104
BstHHI	GCGC	2 cut(s)	103, 878
BstKTI	GATC	4 cut(s)	268, 279, 796, 924
BstMAI	GTCTC	4 cut(s)	684, 910, 951, 1418
BstMBI	GATC	4 cut(s)	265, 276, 793, 921
BstMCI	CGRYCG	1 cut(s)	1487
BstMWI	GCNNNNNNNGC	7 cut(s)	238, 340, 471, 502, 532, 1226, 1333
BstNI	CCWGG	3 cut(s)	901, 1070, 1094
BstNSI	RCATGY	1 cut(s)	808
BstPI	GGTNACC	1 cut(s)	1447
BstSCI	CCNGG	4 cut(s)	93, 899, 1068, 1092
BstSFI	CTRYAG	4 cut(s)	61, 578, 952, 1182
BstSLI	GKGCMC	4 cut(s)	427, 761, 1528, 1689
BstUI	CGCG	2 cut(s)	513, 911
BstV1I	GCAGC	3 cut(s)	62, 70, 92
BstV2I	GAAGAC	2 cut(s)	1071, 1095
BstX2I	RGATCY	2 cut(s)	265, 276
BstXI	CCANNNNNNTGG	3 cut(s)	97, 738, 1406
BstYI	RGATCY	2 cut(s)	265, 276
Bsu15I	ATCGAT	1 cut(s)	324
BsuI	GTATCC	1 cut(s)	301
BsuRI	GGCC	4 cut(s)	950, 1188, 1385, 1439
BsuTUI	ATCGAT	1 cut(s)	324
BtgI	CCRYGG	3 cut(s)	511, 909, 1263
BtgZI	GCGATG	1 cut(s)	886
BtsI	GCAGTG	1 cut(s)	1482
BtsIMutI	CAGTG	3 cut(s)	690, 1389, 1482
Cac8I	GCNNGC	7 cut(s)	78, 628, 810, 1190, 1218, 1387, 1413
CciI	TCATGA	2 cut(s)	763, 1368
CfoI	GCGC	2 cut(s)	103, 878
Cfr10I	RCCGGY	3 cut(s)	103, 1188, 1486
Cfr13I	GGNCC	1 cut(s)	1437
Cfr42I	CCGCGG	2 cut(s)	514, 912
ClaI	ATCGAT	1 cut(s)	324
CsiI	ACCWGGT	2 cut(s)	1068, 1092
Csp6I	GTAC	2 cut(s)	163, 521
CspAI	ACCGGT	1 cut(s)	1486
CviAII	CATG	8 cut(s)	247, 589, 764, 805, 1207, 1369, 1555, 1564
CviQI	GTAC	2 cut(s)	163, 521
DdeI	CTNAG	3 cut(s)	46, 303, 312
DinI	GGCGCC	1 cut(s)	102
DpnI	GATC	4 cut(s)	267, 278, 795, 923
DpnII	GATC	4 cut(s)	265, 276, 793, 921
DriI	GACNNNNNGTC	1 cut(s)	1509
Eam1104I	CTCTTC	1 cut(s)	1655
Eam1105I	GACNNNNNGTC	1 cut(s)	1509
EarI	CTCTTC	1 cut(s)	1655
EciI	GGCGGA	1 cut(s)	1556
Eco130I	CCWWGG	2 cut(s)	961, 1689
Eco147I	AGGCCT	1 cut(s)	950
Eco31I	GGTCTC	2 cut(s)	910, 951
Eco32I	GATATC	2 cut(s)	322, 437
Eco57I	CTGAAG	2 cut(s)	844, 952
Eco72I	CACGTG	1 cut(s)	1471
Eco88I	CYCGRG	1 cut(s)	641
Eco91I	GGTNACC	1 cut(s)	1447
EcoNI	CCTNNNNNAGG	3 cut(s)	61, 624, 1593
EcoO109I	RGGNCCY	1 cut(s)	1437
EcoO65I	GGTNACC	1 cut(s)	1447
EcoRI	GAATTC	2 cut(s)	697, 1297
EcoRII	CCWGG	3 cut(s)	899, 1068, 1092
EcoRV	GATATC	2 cut(s)	322, 437
EcoT14I	CCWWGG	2 cut(s)	961, 1689
EcoT22I	ATGCAT	1 cut(s)	1500
EgeI	GGCGCC	1 cut(s)	102
EheI	GGCGCC	1 cut(s)	102
ErhI	CCWWGG	2 cut(s)	961, 1689
FaeI	CATG	8 cut(s)	250, 592, 767, 808, 1210, 1372, 1558, 1567
FalI	AAGNNNNNCTT	4 cut(s)	1042, 1074, 1314, 1346
FaqI	GGGAC	3 cut(s)	849, 1182, 1652
FatI	CATG	8 cut(s)	246, 588, 763, 804, 1206, 1368, 1554, 1563
Fnu4HI	GCNGC	4 cut(s)	51, 81, 84, 126
Fsp4HI	GCNGC	4 cut(s)	51, 81, 84, 126
FspBI	CTAG	4 cut(s)	141, 944, 1016, 1217
FspI	TGCGCA	1 cut(s)	877
GlaI	GCGC	2 cut(s)	102, 877
GluI	GCNGC	4 cut(s)	51, 81, 84, 126
HaeII	RGCGCY	1 cut(s)	104
HaeIII	GGCC	4 cut(s)	950, 1188, 1385, 1439
HapII	CCGG	6 cut(s)	95, 104, 527, 1189, 1255, 1487
HhaI	GCGC	2 cut(s)	103, 878
Hin1I	GRCGYC	1 cut(s)	101
Hin1II	CATG	8 cut(s)	250, 592, 767, 808, 1210, 1372, 1558, 1567
Hin6I	GCGC	2 cut(s)	101, 876
HinP1I	GCGC	2 cut(s)	101, 876
HincII	GTYRAC	1 cut(s)	1699
HindII	GTYRAC	1 cut(s)	1699
HindIII	AAGCTT	1 cut(s)	239
HinfI	GANTC	6 cut(s)	574, 828, 834, 855, 1480, 1634
HpaII	CCGG	6 cut(s)	95, 104, 527, 1189, 1255, 1487
HphI	GGTGA	5 cut(s)	226, 1084, 1252, 1459, 1501
Hpy166II	GTNNAC	3 cut(s)	425, 1526, 1699
Hpy188I	TCNGA	4 cut(s)	833, 971, 1039, 1087
Hpy188III	TCNNGA	5 cut(s)	206, 764, 1302, 1369, 1705
Hpy8I	GTNNAC	3 cut(s)	425, 1526, 1699
HpyAV	CCTTC	9 cut(s)	169, 205, 245, 1019, 1145, 1368, 1438, 1589, 1739
HpyCH4III	ACNGT	1 cut(s)	1264
HpyCH4IV	ACGT	1 cut(s)	1470
HpyF10VI	GCNNNNNNNGC	7 cut(s)	238, 340, 471, 502, 532, 1226, 1333
HpyF3I	CTNAG	3 cut(s)	46, 303, 312
HpySE526I	ACGT	1 cut(s)	1470
Hsp92I	GRCGYC	1 cut(s)	101
Hsp92II	CATG	8 cut(s)	250, 592, 767, 808, 1210, 1372, 1558, 1567
HspAI	GCGC	2 cut(s)	101, 876
KasI	GGCGCC	1 cut(s)	100
KroI	GCCGGC	1 cut(s)	1188
KroNI	GCCGGC	1 cut(s)	1190
KspI	CCGCGG	2 cut(s)	514, 912
Kzo9I	GATC	4 cut(s)	265, 276, 793, 921
LmnI	GCTCC	4 cut(s)	58, 229, 1267, 1721
Lsp1109I	GCAGC	3 cut(s)	62, 70, 92
LweI	GCATC	5 cut(s)	787, 863, 886, 1088, 1485
MabI	ACCWGGT	2 cut(s)	1068, 1092
MaeI	CTAG	4 cut(s)	141, 944, 1016, 1217
MaeII	ACGT	1 cut(s)	1470
MaeIII	GTNAC	7 cut(s)	3, 412, 484, 718, 1447, 1477, 1631
MalI	GATC	4 cut(s)	267, 278, 795, 923
MboI	GATC	4 cut(s)	265, 276, 793, 921
MboII	GAAGA	9 cut(s)	229, 275, 850, 1074, 1076, 1100, 1632, 1672, 1754
MflI	RGATCY	2 cut(s)	265, 276
MhlI	GDGCHC	4 cut(s)	427, 761, 1528, 1689
MluCI	AATT	7 cut(s)	499, 540, 697, 838, 1297, 1305, 1362
Mly113I	GGCGCC	1 cut(s)	101
MlyI	GAGTC	3 cut(s)	583, 1474, 1628
MmeI	TCCRAC	3 cut(s)	544, 592, 829
Mph1103I	ATGCAT	1 cut(s)	1500
MroNI	GCCGGC	1 cut(s)	1188
MroXI	GAANNNNTTC	1 cut(s)	242
MseI	TTAA	4 cut(s)	198, 1098, 1157, 1756
MslI	CAYNNNNRTG	1 cut(s)	95
MspA1I	CMGCKG	3 cut(s)	83, 513, 911
MspI	CCGG	6 cut(s)	95, 104, 527, 1189, 1255, 1487
MspR9I	CCNGG	4 cut(s)	95, 901, 1070, 1094
Mva1269I	GAATGC	1 cut(s)	1059
MvaI	CCWGG	3 cut(s)	901, 1070, 1094
MvnI	CGCG	2 cut(s)	513, 911
MwoI	GCNNNNNNNGC	7 cut(s)	238, 340, 471, 502, 532, 1226, 1333
NaeI	GCCGGC	1 cut(s)	1190
NarI	GGCGCC	1 cut(s)	101
NciI	CCSGG	1 cut(s)	95
NdeII	GATC	4 cut(s)	265, 276, 793, 921
NgoMIV	GCCGGC	1 cut(s)	1188
NheI	GCTAGC	1 cut(s)	1216
NlaIII	CATG	8 cut(s)	250, 592, 767, 808, 1210, 1372, 1558, 1567
NlaIV	GGNNCC	5 cut(s)	102, 138, 231, 977, 1686
NmeAIII	GCCGAG	1 cut(s)	139
NmuCI	GTSAC	7 cut(s)	3, 412, 484, 718, 1447, 1477, 1631
NsbI	TGCGCA	1 cut(s)	877
NsiI	ATGCAT	1 cut(s)	1500
NspI	RCATGY	1 cut(s)	808
NspV	TTCGAA	1 cut(s)	1062
OliI	CACNNNNGTG	1 cut(s)	95
PaeR7I	CTCGAG	1 cut(s)	641
PagI	TCATGA	2 cut(s)	763, 1368
PceI	AGGCCT	1 cut(s)	950
PcsI	WCGNNNNNNNCGW	1 cut(s)	648
PctI	GAATGC	1 cut(s)	1059
PdiI	GCCGGC	1 cut(s)	1190
PdmI	GAANNNNTTC	1 cut(s)	242
PfeI	GAWTC	3 cut(s)	828, 834, 855
PinAI	ACCGGT	1 cut(s)	1486
PkrI	GCNGC	4 cut(s)	52, 82, 85, 127
PleI	GAGTC	3 cut(s)	582, 1474, 1628
PluTI	GGCGCC	1 cut(s)	104
PmaCI	CACGTG	1 cut(s)	1471
PmlI	CACGTG	1 cut(s)	1471
PpsI	GAGTC	3 cut(s)	582, 1474, 1628
Ppu21I	YACGTR	1 cut(s)	1471
PshBI	ATTAAT	1 cut(s)	198
PsiI	TTATAA	1 cut(s)	1656
Psp6I	CCWGG	3 cut(s)	899, 1068, 1092
PspCI	CACGTG	1 cut(s)	1471
PspEI	GGTNACC	1 cut(s)	1447
PspGI	CCWGG	3 cut(s)	899, 1068, 1092
PspN4I	GGNNCC	5 cut(s)	102, 138, 231, 977, 1686
PspPI	GGNCC	1 cut(s)	1437
PspXI	VCTCGAGB	1 cut(s)	641
PstI	CTGCAG	2 cut(s)	65, 582
PsuI	RGATCY	2 cut(s)	265, 276
PvuII	CAGCTG	1 cut(s)	83
RsaI	GTAC	2 cut(s)	164, 522
RsaNI	GTAC	2 cut(s)	163, 521
RseI	CAYNNNNRTG	1 cut(s)	95
SacII	CCGCGG	2 cut(s)	514, 912
SaqAI	TTAA	4 cut(s)	198, 1098, 1157, 1756
SatI	GCNGC	4 cut(s)	51, 81, 84, 126
Sau3AI	GATC	4 cut(s)	265, 276, 793, 921
Sau96I	GGNCC	1 cut(s)	1437
ScaI	AGTACT	1 cut(s)	522
SchI	GAGTC	3 cut(s)	583, 1474, 1628
ScrFI	CCNGG	4 cut(s)	95, 901, 1070, 1094
SduI	GDGCHC	4 cut(s)	427, 761, 1528, 1689
SexAI	ACCWGGT	2 cut(s)	1068, 1092
SfaNI	GCATC	5 cut(s)	787, 863, 886, 1088, 1485
SfcI	CTRYAG	4 cut(s)	61, 578, 952, 1182
SfoI	GGCGCC	1 cut(s)	102
Sfr274I	CTCGAG	1 cut(s)	641
Sfr303I	CCGCGG	2 cut(s)	514, 912
SfuI	TTCGAA	1 cut(s)	1062
SgrBI	CCGCGG	2 cut(s)	514, 912
SlaI	CTCGAG	1 cut(s)	641
SmiMI	CAYNNNNRTG	1 cut(s)	95
SmlI	CTYRAG	2 cut(s)	335, 641
SmoI	CTYRAG	2 cut(s)	335, 641
Sse9I	AATT	7 cut(s)	499, 540, 697, 838, 1297, 1305, 1362
SseBI	AGGCCT	1 cut(s)	950
SsiI	CCGC	8 cut(s)	125, 479, 511, 513, 909, 911, 1139, 1541
SspDI	GGCGCC	1 cut(s)	100
SspMI	CTAG	4 cut(s)	141, 944, 1016, 1217
StuI	AGGCCT	1 cut(s)	950
StyD4I	CCNGG	4 cut(s)	93, 899, 1068, 1092
StyI	CCWWGG	2 cut(s)	961, 1689
TaaI	ACNGT	1 cut(s)	1264
TaiI	ACGT	1 cut(s)	1473
TaqI	TCGA	6 cut(s)	324, 642, 651, 774, 1062, 1483
TaqII	GACCGA	1 cut(s)	1466
TasI	AATT	7 cut(s)	499, 540, 697, 838, 1297, 1305, 1362
TatI	WGTACW	1 cut(s)	520
TauI	GCSGC	1 cut(s)	128
TfiI	GAWTC	3 cut(s)	828, 834, 855
Tru1I	TTAA	4 cut(s)	198, 1098, 1157, 1756
Tru9I	TTAA	4 cut(s)	198, 1098, 1157, 1756
TscAI	CASTG	3 cut(s)	697, 1396, 1482
TseFI	GTSAC	7 cut(s)	3, 412, 484, 718, 1447, 1477, 1631
TseI	GCWGC	3 cut(s)	50, 80, 83
Tsp45I	GTSAC	7 cut(s)	3, 412, 484, 718, 1447, 1477, 1631
TspDTI	ATGAA	5 cut(s)	235, 251, 1026, 1385, 1543
TspGWI	ACGGA	1 cut(s)	1718
TspRI	CASTG	3 cut(s)	697, 1396, 1482
VneI	GTGCAC	2 cut(s)	423, 1524
VspI	ATTAAT	1 cut(s)	198
XagI	CCTNNNNNAGG	3 cut(s)	61, 624, 1593
XapI	RAATTY	3 cut(s)	540, 697, 1297
XceI	RCATGY	1 cut(s)	808
XcmI	CCANNNNNNNNNTGG	1 cut(s)	94
XhoI	CTCGAG	1 cut(s)	641
XmnI	GAANNNNTTC	1 cut(s)	242
XspI	CTAG	4 cut(s)	141, 944, 1016, 1217
ZrmI	AGTACT	1 cut(s)	522
Zsp2I	ATGCAT	1 cut(s)	1500

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.