Rroxscaffold_7G00208680

Ferredoxin-nitrite reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015213
Physical Location & Seq
Reverse (-)
58815165 .. 58819618
4454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208680.1

Sequence Viewer

Length: 987 bp
ATGTCGTCATTTGGGTTTCTCTTTACTCCTCCTCTGTGCTCCTCGGCTCCCATGAGGCAGCTCGCCGCAACCACCACCCCGGCTGTGGCACCGGTTGTCATGACGGACGTGGCTGCGGAGAGGCTGGAGCCGAGGGTGGAGGAGAGAGAAGGGTATTGGGTGTTGAAGGAGAAGTTTAGAGAAGGGATTAATGTTCAGGAGAAGGTCAAGATTCAGAGGGAGCCTATGAAGCTTTTCATGGAAGGTGGGATTGAAGAGCTTGCAAAGTTGCCTTTTGAGGAGCTTGACAAAGTTAAGGATACTAAGGATGATATTGATGTTAGGCTCAAGTGGCTTGGTCTCTTCCACAGAAGAAAGCATCACTATGGTAGATTTATGATGAGGCTGAAGCTACCAAATGGGGTCACAACAAGTGCACAGACAAGATATCTAGCGAGCGTGATACGAAAATATGGCAAGGATGGTTGTGCAGATGTTACAACCAGGCAAAATTGGCAAATCCGCGGAGTTGTGCTGGCTGATGTACCCGACATTTTGAAGGGTCTTGCTGAAGTAGGCTTGACTAGTCTGCAGAGTGGGATGGACAATGTGAGAAACCCAGTTAGGAATCCTCTTGCAGGCATTGACCCACTTGAAATCGTTGACACAAGTCCTTATACGAACTTGTTGTCCCAATACATTACTGGCAATTCACTTGGCAATCCAACTGTAACAAACTTGCCAAGGAAGTGGAATGTGTGTGTAATAGGATCTCATGATCTTTATGAGCATCCCCACATCAACGATCTTGCATACATGCCTGCAACAAAGGATGGAAAATTTGGATTCAATTTACTCGTGGGAGGCTTCTTCAGTCCCAAAAGATGTGCAGAGGCAGTTCCTCTTGATGCATGGGTCTCGACAGAAGATATGATCCCAGTTTGCAAAGCTATACTAGAGGCTTATAGAGACCTTGGCTTCAGAGGAACAGACAAAAAACAAGAATGA

Protein Analysis

328

Amino Acids

36.87

Weight (kDa)

8.17

Isoelectric Point (pI)

30.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIR_SIR_ferr PF03460 118 - 185 2e-18 Nitrite/Sulfite reductase ferredoxin-like half domain
NIR_SIR PF01077 194 - 326 5e-28 Nitrite and sulphite reductase 4Fe-4S domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015213)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15620
fragaria_vesca FvH4_2g05960
malus_domestica MD05G1081500.v1.1
prunus_persica Prupe.8G126000_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0257431
rosa_laevigata RLG00000014695
rosa_multiflora Rmu_sc0000820.1_g000048 Rmu_sc0003301.1_g000002
rosa_roxburghii Rroxscaffold_7G00208660 Rroxscaffold_7G00208680
rosa_rugosa Rorug05G0572600
rosa_samantha Rh6AG090100 Rh6BG081600 Rh6CG078100 Rh6DG073900
rosa_wichuraiana Rw6G007870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 88
AccII CGCG 1 cut(s) 504
AciI CCGC 4 cut(s) 66, 116, 502, 504
AclWI GGATC 2 cut(s) 757, 907
AcsI RAATTY 1 cut(s) 818
AcuI CTGAAG 4 cut(s) 407, 570, 835, 943
AfaI GTAC 1 cut(s) 525
AfiI CCNNNNNNNGG 2 cut(s) 85, 617
AgeI ACCGGT 1 cut(s) 91
AgsI TTSAA 5 cut(s) 166, 254, 538, 635, 829
AhlI ACTAGT 1 cut(s) 563
AjiI CACGTC 1 cut(s) 109
AjnI CCWGG 1 cut(s) 482
AloI GAACNNNNNNTCC 2 cut(s) 653, 685
AluBI AGCT 6 cut(s) 61, 232, 259, 283, 391, 929
AluI AGCT 6 cut(s) 61, 232, 259, 283, 391, 929
Alw21I GWGCWC 2 cut(s) 41, 418
Alw26I GTCTC 3 cut(s) 344, 901, 942
Alw44I GTGCAC 1 cut(s) 414
AlwI GGATC 2 cut(s) 757, 907
ApaLI GTGCAC 1 cut(s) 414
ApeKI GCWGC 2 cut(s) 58, 113
ApoI RAATTY 1 cut(s) 818
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AseI ATTAAT 1 cut(s) 189
AsiGI ACCGGT 1 cut(s) 91
Asp700I GAANNNNTTC 1 cut(s) 233
AsuC2I CCSGG 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 88
BauI CACGAG 1 cut(s) 836
Bbv12I GWGCWC 2 cut(s) 41, 418
BbvI GCAGC 2 cut(s) 70, 100
BccI CCATC 3 cut(s) 455, 574, 806
BcgI CGANNNNNNTGC 2 cut(s) 879, 913
BciT130I CCWGG 1 cut(s) 484
BciVI GTATCC 1 cut(s) 292
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 3 cut(s) 344, 901, 942
BcuI ACTAGT 1 cut(s) 563
BfaI CTAG 3 cut(s) 431, 564, 935
BfmI CTRYAG 1 cut(s) 569
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 3 cut(s) 59, 66, 114
BlsI GCNGC 3 cut(s) 60, 67, 115
Bme1390I CCNGG 2 cut(s) 80, 484
BmgBI CACGTC 1 cut(s) 109
BmiI GGNNCC 4 cut(s) 48, 90, 129, 222
BmrFI CCNGG 2 cut(s) 80, 484
BmrI ACTGGG 2 cut(s) 593, 911
BmsI GCATC 3 cut(s) 367, 778, 877
BmuI ACTGGG 2 cut(s) 593, 911
BoxI GACNNNNGTC 1 cut(s) 648
BpmI CTGGAG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 80
BsaI GGTCTC 3 cut(s) 344, 901, 942
BsaJI CCNNGG 6 cut(s) 42, 78, 131, 502, 722, 952
BsaWI WCCGGW 1 cut(s) 91
Bsc4I CCNNNNNNNGG 2 cut(s) 85, 617
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 3 cut(s) 599, 688, 917
BseBI CCWGG 1 cut(s) 484
BseDI CCNNGG 6 cut(s) 42, 78, 131, 502, 722, 952
BseGI GGATG 5 cut(s) 313, 466, 585, 769, 817
BseLI CCNNNNNNNGG 2 cut(s) 85, 617
BseNI ACTGG 3 cut(s) 599, 688, 917
BseRI GAGGAG 5 cut(s) 18, 21, 31, 155, 293
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 2 cut(s) 70, 100
BsgI GTGCAG 2 cut(s) 489, 888
Bsh1236I CGCG 1 cut(s) 504
BshNI GGYRCC 1 cut(s) 88
BshTI ACCGGT 1 cut(s) 91
BsiHKAI GWGCWC 2 cut(s) 41, 418
BsiSI CCGG 2 cut(s) 80, 92
BslFI GGGAC 2 cut(s) 655, 840
BslI CCNNNNNNNGG 2 cut(s) 85, 617
BsmAI GTCTC 3 cut(s) 344, 901, 942
BsmFI GGGAC 2 cut(s) 655, 840
Bso31I GGTCTC 3 cut(s) 344, 901, 942
Bsp1286I GDGCHC 2 cut(s) 41, 418
Bsp143I GATC 4 cut(s) 749, 757, 784, 912
BspACI CCGC 4 cut(s) 66, 116, 502, 504
BspFNI CGCG 1 cut(s) 504
BspHI TCATGA 2 cut(s) 99, 754
BspLI GGNNCC 4 cut(s) 48, 90, 129, 222
BspMAI CTGCAG 1 cut(s) 573
BspPI GGATC 2 cut(s) 757, 907
BspQI GCTCTTC 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 88
BspTNI GGTCTC 3 cut(s) 344, 901, 942
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 3 cut(s) 599, 688, 917
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 6 cut(s) 42, 78, 131, 502, 722, 952
BssMI GATC 4 cut(s) 749, 757, 784, 912
BssSI CACGAG 1 cut(s) 836
BssT1I CCWWGG 2 cut(s) 722, 952
Bst2BI CACGAG 1 cut(s) 836
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 709
Bst6I CTCTTC 2 cut(s) 249, 347
BstC8I GCNNGC 6 cut(s) 63, 261, 436, 516, 619, 801
BstDEI CTNAG 1 cut(s) 303
BstDSI CCRYGG 1 cut(s) 502
BstENI CCTNNNNNAGG 1 cut(s) 615
BstF5I GGATG 5 cut(s) 313, 466, 585, 769, 817
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 4 cut(s) 752, 760, 787, 915
BstMAI GTCTC 3 cut(s) 344, 901, 942
BstMBI GATC 4 cut(s) 749, 757, 784, 912
BstMWI GCNNNNNNNGC 3 cut(s) 229, 331, 493
BstNI CCWGG 1 cut(s) 484
BstNSI RCATGY 1 cut(s) 799
BstPAI GACNNNNGTC 1 cut(s) 648
BstSCI CCNGG 2 cut(s) 78, 482
BstSFI CTRYAG 1 cut(s) 569
BstSLI GKGCMC 1 cut(s) 418
BstUI CGCG 1 cut(s) 504
BstV1I GCAGC 2 cut(s) 70, 100
BstX2I RGATCY 1 cut(s) 749
BstXI CCANNNNNNTGG 1 cut(s) 729
BstYI RGATCY 1 cut(s) 749
BsuI GTATCC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 502
BtrI CACGTC 1 cut(s) 109
BtsCI GGATG 5 cut(s) 313, 466, 585, 769, 817
Cac8I GCNNGC 6 cut(s) 63, 261, 436, 516, 619, 801
CciI TCATGA 2 cut(s) 99, 754
Cfr10I RCCGGY 1 cut(s) 91
Cfr42I CCGCGG 1 cut(s) 505
Csp6I GTAC 1 cut(s) 524
CspAI ACCGGT 1 cut(s) 91
CviAII CATG 6 cut(s) 52, 100, 238, 755, 796, 891
CviQI GTAC 1 cut(s) 524
DdeI CTNAG 1 cut(s) 303
DpnI GATC 4 cut(s) 751, 759, 786, 914
DpnII GATC 4 cut(s) 749, 757, 784, 912
Eam1104I CTCTTC 2 cut(s) 249, 347
EarI CTCTTC 2 cut(s) 249, 347
Eco130I CCWWGG 2 cut(s) 722, 952
Eco31I GGTCTC 3 cut(s) 344, 901, 942
Eco32I GATATC 1 cut(s) 428
Eco57I CTGAAG 4 cut(s) 407, 570, 835, 943
EcoNI CCTNNNNNAGG 1 cut(s) 615
EcoRII CCWGG 1 cut(s) 482
EcoRV GATATC 1 cut(s) 428
EcoT14I CCWWGG 2 cut(s) 722, 952
EcoT22I ATGCAT 1 cut(s) 892
ErhI CCWWGG 2 cut(s) 722, 952
FaeI CATG 6 cut(s) 55, 103, 241, 758, 799, 894
FaqI GGGAC 2 cut(s) 655, 840
FatI CATG 6 cut(s) 51, 99, 237, 754, 795, 890
Fnu4HI GCNGC 3 cut(s) 59, 66, 114
FokI GGATG 5 cut(s) 320, 473, 592, 756, 824
Fsp4HI GCNGC 3 cut(s) 59, 66, 114
FspBI CTAG 3 cut(s) 431, 564, 935
GluI GCNGC 3 cut(s) 59, 66, 114
GsuI CTGGAG 1 cut(s) 146
HapII CCGG 2 cut(s) 80, 92
Hin1II CATG 6 cut(s) 55, 103, 241, 758, 799, 894
HincII GTYRAC 1 cut(s) 643
HindII GTYRAC 1 cut(s) 643
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 3 cut(s) 211, 607, 825
HpaII CCGG 2 cut(s) 80, 92
Hpy166II GTNNAC 2 cut(s) 416, 643
Hpy188I TCNGA 2 cut(s) 216, 962
Hpy188III TCNNGA 6 cut(s) 100, 197, 208, 755, 884, 898
Hpy8I GTNNAC 2 cut(s) 416, 643
HpyAV CCTTC 6 cut(s) 143, 160, 176, 196, 236, 532
HpyCH4III ACNGT 1 cut(s) 709
HpyCH4IV ACGT 1 cut(s) 108
HpyF10VI GCNNNNNNNGC 3 cut(s) 229, 331, 493
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 6 cut(s) 55, 103, 241, 758, 799, 894
KspI CCGCGG 1 cut(s) 505
Kzo9I GATC 4 cut(s) 749, 757, 784, 912
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 5 cut(s) 44, 52, 127, 220, 280
Lsp1109I GCAGC 2 cut(s) 70, 100
LweI GCATC 3 cut(s) 367, 778, 877
MaeI CTAG 3 cut(s) 431, 564, 935
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 3 cut(s) 403, 475, 709
MalI GATC 4 cut(s) 751, 759, 786, 914
MboI GATC 4 cut(s) 749, 757, 784, 912
MboII GAAGA 5 cut(s) 266, 334, 363, 841, 917
MflI RGATCY 1 cut(s) 749
MhlI GDGCHC 2 cut(s) 41, 418
MluCI AATT 4 cut(s) 490, 688, 818, 829
MmeI TCCRAC 1 cut(s) 728
Mph1103I ATGCAT 1 cut(s) 892
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 2 cut(s) 189, 294
MslI CAYNNNNRTG 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 504
MspI CCGG 2 cut(s) 80, 92
MspR9I CCNGG 2 cut(s) 80, 484
MvaI CCWGG 1 cut(s) 484
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 3 cut(s) 229, 331, 493
NciI CCSGG 1 cut(s) 80
NdeII GATC 4 cut(s) 749, 757, 784, 912
NlaIII CATG 6 cut(s) 55, 103, 241, 758, 799, 894
NlaIV GGNNCC 4 cut(s) 48, 90, 129, 222
NmeAIII GCCGAG 2 cut(s) 23, 156
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 892
NspI RCATGY 1 cut(s) 799
PagI TCATGA 2 cut(s) 99, 754
PciSI GCTCTTC 1 cut(s) 249
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 3 cut(s) 211, 607, 825
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 3 cut(s) 60, 67, 115
PshAI GACNNNNGTC 1 cut(s) 648
PshBI ATTAAT 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 482
PspGI CCWGG 1 cut(s) 482
PspN4I GGNNCC 4 cut(s) 48, 90, 129, 222
PstI CTGCAG 1 cut(s) 573
PsuI RGATCY 1 cut(s) 749
RsaI GTAC 1 cut(s) 525
RsaNI GTAC 1 cut(s) 524
RseI CAYNNNNRTG 1 cut(s) 363
SacII CCGCGG 1 cut(s) 505
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 2 cut(s) 189, 294
SatI GCNGC 3 cut(s) 59, 66, 114
Sau3AI GATC 4 cut(s) 749, 757, 784, 912
ScrFI CCNGG 2 cut(s) 80, 484
SduI GDGCHC 2 cut(s) 41, 418
SfaNI GCATC 3 cut(s) 367, 778, 877
SfcI CTRYAG 1 cut(s) 569
Sfr303I CCGCGG 1 cut(s) 505
SgrBI CCGCGG 1 cut(s) 505
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SpeI ACTAGT 1 cut(s) 563
Sse9I AATT 4 cut(s) 490, 688, 818, 829
SsiI CCGC 4 cut(s) 66, 116, 502, 504
SspMI CTAG 3 cut(s) 431, 564, 935
StyD4I CCNGG 2 cut(s) 78, 482
StyI CCWWGG 2 cut(s) 722, 952
TaaI ACNGT 1 cut(s) 709
TaiI ACGT 1 cut(s) 111
TaqI TCGA 1 cut(s) 899
TasI AATT 4 cut(s) 490, 688, 818, 829
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 3 cut(s) 211, 607, 825
Tru1I TTAA 2 cut(s) 189, 294
Tru9I TTAA 2 cut(s) 189, 294
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 2 cut(s) 58, 113
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 226, 242
TspGWI ACGGA 1 cut(s) 119
VneI GTGCAC 1 cut(s) 414
VspI ATTAAT 1 cut(s) 189
XagI CCTNNNNNAGG 1 cut(s) 615
XapI RAATTY 1 cut(s) 818
XceI RCATGY 1 cut(s) 799
XcmI CCANNNNNNNNNTGG 2 cut(s) 82, 680
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 3 cut(s) 431, 564, 935
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.