MD05G1096400.v1.1

Somatic embryogenesis receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015530
Physical Location & Seq
Forward (+)
20274792 .. 20277423
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1096400.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGCTCCTCTCTCCTTACTACCTTTCTCTCTCACCTTCCTCTTCTTTCTCTCTCCTGCGCTCTCCACAAACTCCGAAGGAAATGCTTTGCATGCCCTGAGAAGCAGATTCAACGATGTCAACAACGTTCTGCAGAGCTGGGATCCAACGCTGGTCAATCCCTGCACCTGGTTCCACGTTACCTGTGACTCCAACAACCATGTCATCCGATTGGATTTGGGTAACTCTAACATTTCTGGGAGTTTGGGGCCAGAACTTGGGCAGCTGAAGCACCTGCAGTACTTGGAACTTTATAGAAACGACATAAGTGGTAAAATCCCAGAGGAGTTGGGGAGCTTGAAAAGCCTTGTTAGCATGGATTTGTATGACAACAGGTTTGAAGGAGAAATCCCGAAATCTTTCGCCAATTTGAAGTCACTCAGATTTCTGCGGCTTAACAACAACAAACTAACGGGATCTATCCCGAGGAAACTCGTTGGCCTCTCTGACCTCAAAGTTTTTGATGTTTCTAACAACGATCTGTGCGGAACAATTCCAGTTGATGGTCCATTTGGGGCTTTCCCAACGGAAAGTTTTGAAAACAACAGACTTAACGGCCCCGAGCTGAAAGGGCTGGTATCCTACGACTTTGGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007030 GO:0007049 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009555 GO:0009556 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009838 GO:0009908 GO:0009987 GO:0010033 GO:0010152 GO:0010154 GO:0010227 GO:0010256 GO:0012505 GO:0014070 GO:0016020 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019953 GO:0021700 GO:0022402 GO:0022414 GO:0023052 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0033612 GO:0033993 GO:0034293 GO:0036211 GO:0038023 GO:0042175 GO:0042221 GO:0042802 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043401 GO:0043412 GO:0043934 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044703 GO:0046777 GO:0048229 GO:0048236 GO:0048316 GO:0048367 GO:0048437 GO:0048545 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051704 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071840 GO:0071944 GO:0090567 GO:0098827 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1903046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8

Protein Analysis

213

Amino Acids

23.51

Weight (kDa)

5.55

Isoelectric Point (pI)

34.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 4.3e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 70 - 126 5.4e-06 Leucine rich repeat
LRR_14 PF23598 71 - 171 6.7e-13 Leucine-rich repeat region
LRR_8 PF13855 131 - 174 2.4e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015530)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03940
malus_domestica MD05G1096400.v1.1 MD10G1102700.v1.1
prunus_persica Prupe.8G140900_v2.0.a1
pyrus_communis pycom05g09520
rosa_chinensis RchiOBHm_Chr6g0252991
rosa_laevigata RLG00000015046
rosa_multiflora Rmu_co8514715.1_g000001 Rmu_sc0002560.1_g000007
rosa_roxburghii Rroxscaffold_7G00212240
rosa_rugosa Rorug05G0542400
rosa_samantha Rh6AG056500 Rh6BG050900 Rh6CG048800 Rh6DG044500
rosa_wichuraiana Rw6G005160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 282
Acc36I ACCTGC 1 cut(s) 282
AccB7I CCANNNNNTGG 2 cut(s) 257, 542
AciI CCGC 2 cut(s) 430, 525
AclI AACGTT 1 cut(s) 126
AclWI GGATC 3 cut(s) 137, 150, 463
AcuI CTGAAG 1 cut(s) 287
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 168, 257, 542
AgsI TTSAA 5 cut(s) 112, 340, 380, 412, 578
AjnI CCWGG 1 cut(s) 167
AluBI AGCT 4 cut(s) 138, 265, 336, 604
AluI AGCT 4 cut(s) 138, 265, 336, 604
AlwI GGATC 3 cut(s) 137, 150, 463
Ama87I CYCGRG 2 cut(s) 463, 599
AoxI GGCC 3 cut(s) 248, 478, 595
ApeKI GCWGC 1 cut(s) 262
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
Asp700I GAANNNNTTC 1 cut(s) 398
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 3 cut(s) 248, 545, 596
AsuHPI GGTGA 1 cut(s) 25
AvaI CYCGRG 2 cut(s) 463, 599
AvaII GGWCC 1 cut(s) 545
BamHI GGATCC 1 cut(s) 142
BbvI GCAGC 1 cut(s) 274
BccI CCATC 1 cut(s) 536
BceAI ACGGC 1 cut(s) 610
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 1 cut(s) 169
BciVI GTATCC 1 cut(s) 628
BfmI CTRYAG 2 cut(s) 131, 275
BfuAI ACCTGC 1 cut(s) 282
BfuI GTATCC 1 cut(s) 628
BisI GCNGC 2 cut(s) 263, 431
BlsI GCNGC 2 cut(s) 264, 432
BmcAI AGTACT 1 cut(s) 281
Bme1390I CCNGG 1 cut(s) 169
Bme18I GGWCC 1 cut(s) 545
BmeT110I CYCGRG 2 cut(s) 463, 599
BmgT120I GGNCC 3 cut(s) 248, 545, 596
BmiI GGNNCC 5 cut(s) 6, 144, 173, 249, 598
BmrFI CCNGG 1 cut(s) 169
BsaJI CCNNGG 1 cut(s) 464
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 257, 542
Bse1I ACTGG 1 cut(s) 536
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 1 cut(s) 464
BseGI GGATG 1 cut(s) 204
BseLI CCNNNNNNNGG 3 cut(s) 168, 257, 542
BseMII CTCAG 2 cut(s) 89, 433
BseNI ACTGG 1 cut(s) 536
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 274
BseYI CCCAGC 1 cut(s) 138
BsgI GTGCAG 1 cut(s) 148
BshFI GGCC 3 cut(s) 250, 480, 597
BsiHKCI CYCGRG 2 cut(s) 463, 599
BslI CCNNNNNNNGG 3 cut(s) 168, 257, 542
BsnI GGCC 3 cut(s) 250, 480, 597
BsoBI CYCGRG 2 cut(s) 463, 599
Bsp143I GATC 3 cut(s) 142, 455, 517
BspACI CCGC 2 cut(s) 430, 525
BspANI GGCC 3 cut(s) 250, 480, 597
BspCNI CTCAG 2 cut(s) 90, 432
BspLI GGNNCC 5 cut(s) 6, 144, 173, 249, 598
BspMAI CTGCAG 2 cut(s) 135, 279
BspMI ACCTGC 1 cut(s) 282
BspPI GGATC 3 cut(s) 137, 150, 463
BsrI ACTGG 1 cut(s) 536
BssECI CCNNGG 1 cut(s) 464
BssMI GATC 3 cut(s) 142, 455, 517
Bst2UI CCWGG 1 cut(s) 169
Bst6I CTCTTC 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 98, 419
BstF5I GGATG 1 cut(s) 204
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 3 cut(s) 145, 458, 520
BstMBI GATC 3 cut(s) 142, 455, 517
BstMWI GCNNNNNNNGC 5 cut(s) 92, 268, 342, 351, 610
BstNI CCWGG 1 cut(s) 169
BstNSI RCATGY 1 cut(s) 95
BstSCI CCNGG 1 cut(s) 167
BstSFI CTRYAG 2 cut(s) 131, 275
BstV1I GCAGC 1 cut(s) 274
BstX2I RGATCY 2 cut(s) 142, 455
BstYI RGATCY 2 cut(s) 142, 455
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 3 cut(s) 250, 480, 597
BtsCI GGATG 1 cut(s) 204
BveI ACCTGC 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 3 cut(s) 248, 545, 596
CsiI ACCWGGT 1 cut(s) 167
Csp6I GTAC 1 cut(s) 280
CviAII CATG 3 cut(s) 92, 200, 355
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 2 cut(s) 98, 419
DpnI GATC 3 cut(s) 144, 457, 519
DpnII GATC 3 cut(s) 142, 455, 517
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 545
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 2 cut(s) 463, 599
EcoRII CCWGG 1 cut(s) 167
FaeI CATG 3 cut(s) 95, 203, 358
FaiI YATR 6 cut(s) 93, 201, 294, 305, 356, 366
FatI CATG 3 cut(s) 91, 199, 354
Fnu4HI GCNGC 2 cut(s) 263, 431
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 263, 431
GlaI GCGC 1 cut(s) 60
GluI GCNGC 2 cut(s) 263, 431
GsaI CCCAGC 1 cut(s) 142
HaeIII GGCC 3 cut(s) 250, 480, 597
HhaI GCGC 1 cut(s) 61
Hin1II CATG 3 cut(s) 95, 203, 358
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 2 cut(s) 108, 188
HphI GGTGA 1 cut(s) 25
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 4 cut(s) 76, 209, 422, 487
Hpy188III TCNNGA 2 cut(s) 391, 463
Hpy8I GTNNAC 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 46, 71, 374
HpyCH4IV ACGT 2 cut(s) 126, 177
HpyCH4V TGCA 4 cut(s) 91, 133, 165, 277
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 268, 342, 351, 610
HpyF3I CTNAG 2 cut(s) 98, 419
HpySE526I ACGT 2 cut(s) 126, 177
Hsp92II CATG 3 cut(s) 95, 203, 358
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 3 cut(s) 142, 455, 517
LmnI GCTCC 2 cut(s) 10, 333
Lsp1109I GCAGC 1 cut(s) 274
MabI ACCWGGT 1 cut(s) 167
MaeII ACGT 2 cut(s) 126, 177
MaeIII GTNAC 4 cut(s) 178, 185, 221, 414
MalI GATC 3 cut(s) 144, 457, 519
MboI GATC 3 cut(s) 142, 455, 517
MboII GAAGA 1 cut(s) 34
MflI RGATCY 2 cut(s) 142, 455
MluCI AATT 2 cut(s) 406, 531
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 2 cut(s) 170, 216
MnlI CCTC 6 cut(s) 18, 50, 316, 459, 491, 500
MroXI GAANNNNTTC 1 cut(s) 398
MseI TTAA 2 cut(s) 435, 591
MspA1I CMGCKG 1 cut(s) 265
MspR9I CCNGG 1 cut(s) 169
MvaI CCWGG 1 cut(s) 169
MwoI GCNNNNNNNGC 5 cut(s) 92, 268, 342, 351, 610
NdeII GATC 3 cut(s) 142, 455, 517
NlaIII CATG 3 cut(s) 95, 203, 358
NlaIV GGNNCC 5 cut(s) 6, 144, 173, 249, 598
NmuCI GTSAC 2 cut(s) 185, 414
NspI RCATGY 1 cut(s) 95
PaeI GCATGC 1 cut(s) 95
PaqCI CACCTGC 1 cut(s) 282
PdmI GAANNNNTTC 1 cut(s) 398
PfeI GAWTC 1 cut(s) 108
PflMI CCANNNNNTGG 2 cut(s) 257, 542
PkrI GCNGC 2 cut(s) 264, 432
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp1406I AACGTT 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 167
PspN4I GGNNCC 5 cut(s) 6, 144, 173, 249, 598
PspPI GGNCC 3 cut(s) 248, 545, 596
PstI CTGCAG 2 cut(s) 135, 279
PsuI RGATCY 2 cut(s) 142, 455
PvuII CAGCTG 1 cut(s) 265
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SaqAI TTAA 2 cut(s) 435, 591
SatI GCNGC 2 cut(s) 263, 431
Sau3AI GATC 3 cut(s) 142, 455, 517
Sau96I GGNCC 3 cut(s) 248, 545, 596
ScaI AGTACT 1 cut(s) 281
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 1 cut(s) 169
SexAI ACCWGGT 1 cut(s) 167
SfcI CTRYAG 2 cut(s) 131, 275
SinI GGWCC 1 cut(s) 545
SphI GCATGC 1 cut(s) 95
Sse9I AATT 2 cut(s) 406, 531
SsiI CCGC 2 cut(s) 430, 525
StyD4I CCNGG 1 cut(s) 167
TaiI ACGT 2 cut(s) 129, 180
TasI AATT 2 cut(s) 406, 531
TatI WGTACW 1 cut(s) 279
TauI GCSGC 1 cut(s) 433
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 2 cut(s) 435, 591
Tru9I TTAA 2 cut(s) 435, 591
TseFI GTSAC 2 cut(s) 185, 414
TseI GCWGC 1 cut(s) 262
Tsp45I GTSAC 2 cut(s) 185, 414
TspGWI ACGGA 1 cut(s) 581
Van91I CCANNNNNTGG 2 cut(s) 257, 542
VpaK11BI GGWCC 1 cut(s) 545
XceI RCATGY 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 398
ZrmI AGTACT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.