Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0006384

Physical Location & Seq

Genomic Coordinates Reverse (-)

22045851 .. 22049303

Gene Structure 3453 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1107300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1731 bp

ATGGCGGTCGCGGACCATTCGATCCATACGTGTTTTGGTGTCAAAGCCAATAACCCAAGTGCCAATTTACAGAAAACTGTTTTTGGTGTGCCCGTTTTGCACCATGGGGTGTCCTGTATTGGGAAGGAATTGAAAAGGAAAATTGGTGGTGGTGGAGTGCAGGCGGTGCAAGTGGGTTTGGCGGCGGCAGAGAGTTTGAAAAGTTTTAAGAGTTTGGATCTTGATGTGGTGTCGGAGAGGGAGTTGAGGGAGAAGGGGTTTTTGGGGTTGAGGAAGACCAAACTTGTGTGCACAATTGGGCCGGCGTGTTCTTCTGTGGAGGATTTGGAGAAGTTGGTTTTGGGAGGGATGAATGTGGCAAGGCTGAATATGTGTCACAACACGCGGGAGTGGCACCGCGACGTCATTAGGAAGATCAAGAGGTTGAATGAGGAGAAGGGGTTTTCTGTTTCTGTTATGATCGATACCGAAGGTACTCAGATTCATGTTGTTGATCATGGAGCTCCATCGTCTCTTAAAGTTCAGGAGGATTCAATCTGGCTATTTACCACTGAAAAATTTGAGGGTTCGCTTCCATTCACAGTACAAGCAAATTATGAAGGTTTTTCTGATGGTATCAAAGAGGGTGATGAGCTTGTTATTGATGGAGGAATGGCATGCTTTGAAGTCATTGAAAAATTAGGAAGCGACCTGCGTTGTAAGTGCACAGACCCCGGTCTATTCCTGCCTCGAGCCAAATTCAGCTTTTGGAGAGATGGGAAGCTTGTGGAGAGGAACTATGACCTCCCAACTCTGTCTACTAAGGATTGGTCTGACATTGAATTCGGAATCTCTGAAGGCGTCAATTTTATTGCCATGTCATTTGTAAATGACGCTGAATCTGTGAAGCATTTGAAGAATTACCTCATCACCAAATCAGCCAAATCCACAAGAGTTTTGGCAAAGATTGAGAGTTTGGAAGCCCTTCAGAATTTGGAAGAAATTATAAAGGCTTCTGATGGAATCATGATTGCGCGAGTTGACCTTGGAGTTGAGATCCCACTTGAACAGATTCCAACAGTCCAAGAGGAAATAACCAATGTCTCCAGGCAGTTAAACAAGCCGGTAATTGTAGCTTCTCAGTTTCTTGAGTCAATGATTGAATACCCAACTCCAACGCGTGCTGAGGTTGCAGATGTTTCTGAAGCTGTTCGACAATATGCAGATGCATTGATGTTGGCCGGGGAATCAGCAATTGGATTATTTGGAGAGAAGGCTCTTTCTGTTCTTCAAACGACCAGTAGTAGAATGGAAACATGGAGTCGTGTGGAAAACCAGCAAGGTTCTCTCCTTCAGCGCCAACTAGGAGTCTCGTTGCCTGACCGCATTGCTGAGCAGATATGCAATTCAGCTGTTGAAATGGCTAACAGCCTTGCTGTGGATGCAATCTTTGTTTACACCAAGCATGGGCACATGGCATCGCTCTTGTCACGAAACAGACCAAACCCTCCCATATTTGCTTTCACAAACGATGAAAGCACCCGTATGGCTCTGAATTTGCAGTGGGGAGTGATCCCTCTTCTTGTTAATCTATCGGATGATATGGAGTCTAACATCTCAAGCACCATCCATCTCATAAAATCAAAGGGATTGGTGAAACAAGGAGATTGGGTCTTGGTGGTCTCGGATGTCACTCCTTCCCGCGCTACTCCGATGGCATTCCAATCAATCCAGGTGAAGACCATTGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230

Pfam Domains

Protein Families

PK PK_C

Protein Analysis

577

Amino Acids

63.35

Weight (kDa)

5.68

Isoelectric Point (pI)

37.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK	PF00224	91 - 419	5.6e-81	Pyruvate kinase, barrel domain
PK_C	PF02887	458 - 558	3.2e-24	Pyruvate kinase, alpha/beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006384)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g03100 FvH4_2g03100 FvH4_2g03100 FvH4_2g03100
malus_domestica	MD00G1031300.v1.1 MD05G1107300.v1.1
prunus_persica	Prupe.8G151100_v2.0.a1 Prupe.8G151100_v2.0.a1 Prupe.8G151100_v2.0.a1 Prupe.8G151100_v2.0.a1 Prupe.8G151100_v2.0.a1 Prupe.8G151100_v2.0.a1 Prupe.8G151100_v2.0.a1
pyrus_communis	pycom05g10390 pycom10g09680
rosa_chinensis	RchiOBHm_Chr6g0247971
rosa_laevigata	RLG00000015166
rosa_multiflora	Rmu_sc0010633.1_g000004 Rmu_sc0030265.1_g000001
rosa_roxburghii	Rroxscaffold_179G00449310 Rroxscaffold_7G00213540
rosa_rugosa	Rorug05G0529900
rosa_samantha	Rh6AG044700 Rh6BG039700 Rh6CG038300 Rh6DG035300
rosa_wichuraiana	Rw6G003890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	986
AatII	GACGTC	1 cut(s)	405
Acc36I	ACCTGC	1 cut(s)	699
AccB1I	GGYRCC	1 cut(s)	393
AccI	GTMKAC	1 cut(s)	797
AccII	CGCG	6 cut(s)	11, 385, 399, 1015, 1159, 1683
AciI	CCGC	9 cut(s)	5, 11, 164, 182, 185, 385, 397, 1363, 1681
AclWI	GGATC	4 cut(s)	16, 225, 1030, 1546
AcoI	YGGCCR	1 cut(s)	1218
AcsI	RAATTY	5 cut(s)	557, 737, 821, 970, 1534
AcuI	CTGAAG	4 cut(s)	855, 950, 1203, 1316
AcyI	GRCGYC	2 cut(s)	402, 840
AfaI	GTAC	2 cut(s)	475, 585
AfiI	CCNNNNNNNGG	3 cut(s)	120, 1417, 1446
AflIII	ACRYGT	2 cut(s)	29, 1157
AjnI	CCWGG	2 cut(s)	1085, 1710
AjuI	GAANNNNNNNTTGG	6 cut(s)	245, 277, 323, 355, 1218, 1250
AluBI	AGCT	7 cut(s)	503, 634, 744, 763, 1115, 1187, 1391
AluI	AGCT	7 cut(s)	503, 634, 744, 763, 1115, 1187, 1391
Alw21I	GWGCWC	3 cut(s)	293, 505, 707
Alw26I	GTCTC	4 cut(s)	516, 1087, 1354, 1666
Alw44I	GTGCAC	2 cut(s)	289, 703
AlwI	GGATC	4 cut(s)	16, 225, 1030, 1546
Ama87I	CYCGRG	1 cut(s)	729
AoxI	GGCC	2 cut(s)	299, 1218
ApaLI	GTGCAC	2 cut(s)	289, 703
ApoI	RAATTY	5 cut(s)	557, 737, 821, 970, 1534
ArsI	GACNNNNNNTTYG	2 cut(s)	806, 838
Asp700I	GAANNNNTTC	3 cut(s)	963, 1050, 1188
AspLEI	GCGC	3 cut(s)	1015, 1338, 1685
AspS9I	GGNCC	2 cut(s)	13, 299
AsuC2I	CCSGG	2 cut(s)	714, 1222
AsuHPI	GGTGA	4 cut(s)	638, 901, 1645, 1726
AvaI	CYCGRG	1 cut(s)	729
AvaII	GGWCC	1 cut(s)	13
BaeGI	GKGCMC	4 cut(s)	93, 293, 707, 1452
BanI	GGYRCC	1 cut(s)	393
BanII	GRGCYC	1 cut(s)	505
BbsI	GAAGAC	2 cut(s)	281, 1724
Bbv12I	GWGCWC	3 cut(s)	293, 505, 707
BbvCI	CCTCAGC	1 cut(s)	1164
BccI	CCATC	8 cut(s)	514, 605, 638, 749, 992, 1613, 1617, 1687
BciT130I	CCWGG	2 cut(s)	1087, 1712
BclI	TGATCA	1 cut(s)	493
BcnI	CCSGG	2 cut(s)	714, 1222
BcoDI	GTCTC	4 cut(s)	516, 1087, 1354, 1666
BfaI	CTAG	1 cut(s)	1343
BfoI	RGCGCY	1 cut(s)	1339
BfuAI	ACCTGC	1 cut(s)	699
BisI	GCNGC	2 cut(s)	183, 186
BlpI	GCTNAGC	1 cut(s)	1371
BlsI	GCNGC	2 cut(s)	184, 187
Bme1390I	CCNGG	4 cut(s)	714, 1087, 1222, 1712
Bme18I	GGWCC	1 cut(s)	13
BmeT110I	CYCGRG	1 cut(s)	729
BmgT120I	GGNCC	2 cut(s)	13, 299
BmiI	GGNNCC	1 cut(s)	395
BmrFI	CCNGG	4 cut(s)	714, 1087, 1222, 1712
BmsI	GCATC	3 cut(s)	1195, 1411, 1466
BoxI	GACNNNNGTC	1 cut(s)	714
BpiI	GAAGAC	2 cut(s)	281, 1724
BpmI	CTGGAG	1 cut(s)	1069
Bpu10I	CCTNAGC	1 cut(s)	1164
Bpu1102I	GCTNAGC	1 cut(s)	1371
BpuEI	CTTGAG	2 cut(s)	1148, 1582
BpuMI	CCSGG	2 cut(s)	714, 1222
Bsa29I	ATCGAT	1 cut(s)	462
BsaAI	YACGTR	1 cut(s)	30
BsaHI	GRCGYC	2 cut(s)	402, 840
BsaI	GGTCTC	1 cut(s)	1666
BsaJI	CCNNGG	4 cut(s)	103, 712, 1024, 1221
BsaXI	ACNNNNNCTCC	8 cut(s)	227, 242, 257, 272, 425, 455, 1635, 1665
Bsc4I	CCNNNNNNNGG	3 cut(s)	120, 1417, 1446
Bse118I	RCCGGY	2 cut(s)	301, 1102
Bse1I	ACTGG	1 cut(s)	1278
Bse3DI	GCAATG	1 cut(s)	1365
BseBI	CCWGG	2 cut(s)	1087, 1712
BseCI	ATCGAT	1 cut(s)	462
BseDI	CCNNGG	4 cut(s)	103, 712, 1024, 1221
BseGI	GGATG	5 cut(s)	354, 1426, 1582, 1605, 1672
BseLI	CCNNNNNNNGG	3 cut(s)	120, 1417, 1446
BseMI	GCAATG	1 cut(s)	1365
BseMII	CTCAG	4 cut(s)	491, 1133, 1155, 1362
BseNI	ACTGG	1 cut(s)	1278
BseRI	GAGGAG	1 cut(s)	446
BseSI	GKGCMC	4 cut(s)	93, 293, 707, 1452
BsgI	GTGCAG	1 cut(s)	179
Bsh1236I	CGCG	6 cut(s)	11, 385, 399, 1015, 1159, 1683
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	2 cut(s)	301, 1220
BshNI	GGYRCC	1 cut(s)	393
BshVI	ATCGAT	1 cut(s)	462
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	3 cut(s)	293, 505, 707
BsiHKCI	CYCGRG	1 cut(s)	729
BsiSI	CCGG	4 cut(s)	302, 714, 1103, 1221
BslI	CCNNNNNNNGG	3 cut(s)	120, 1417, 1446
BsmAI	GTCTC	4 cut(s)	516, 1087, 1354, 1666
BsmBI	CGTCTC	1 cut(s)	516
BsmI	GAATGC	1 cut(s)	1697
BsnI	GGCC	2 cut(s)	301, 1220
Bso31I	GGTCTC	1 cut(s)	1666
BsoBI	CYCGRG	1 cut(s)	729
Bsp1286I	GDGCHC	5 cut(s)	93, 293, 505, 707, 1452
Bsp143I	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
Bsp1720I	GCTNAGC	1 cut(s)	1371
Bsp19I	CCATGG	1 cut(s)	103
BspACI	CCGC	9 cut(s)	5, 11, 164, 182, 185, 385, 397, 1363, 1681
BspANI	GGCC	2 cut(s)	301, 1220
BspCNI	CTCAG	4 cut(s)	490, 1132, 1156, 1363
BspDI	ATCGAT	1 cut(s)	462
BspFNI	CGCG	6 cut(s)	11, 385, 399, 1015, 1159, 1683
BspHI	TCATGA	1 cut(s)	1005
BspLI	GGNNCC	1 cut(s)	395
BspMI	ACCTGC	1 cut(s)	699
BspPI	GGATC	4 cut(s)	16, 225, 1030, 1546
BspT107I	GGYRCC	1 cut(s)	393
BspTNI	GGTCTC	1 cut(s)	1666
BsrDI	GCAATG	1 cut(s)	1365
BsrFI	RCCGGY	2 cut(s)	301, 1102
BsrI	ACTGG	1 cut(s)	1278
BssAI	RCCGGY	2 cut(s)	301, 1102
BssECI	CCNNGG	4 cut(s)	103, 712, 1024, 1221
BssMI	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
BssNI	GRCGYC	2 cut(s)	402, 840
BssT1I	CCWWGG	2 cut(s)	103, 1024
Bst2UI	CCWGG	2 cut(s)	1087, 1712
Bst4CI	ACNGT	3 cut(s)	79, 583, 1060
Bst6I	CTCTTC	1 cut(s)	1563
BstACI	GRCGYC	2 cut(s)	402, 840
BstAPI	GCANNNNNTGC	1 cut(s)	166
BstBAI	YACGTR	1 cut(s)	30
BstC8I	GCNNGC	4 cut(s)	162, 303, 658, 1161
BstDEI	CTNAG	5 cut(s)	477, 801, 1119, 1164, 1371
BstDSI	CCRYGG	1 cut(s)	103
BstF5I	GGATG	5 cut(s)	354, 1426, 1582, 1605, 1672
BstFNI	CGCG	6 cut(s)	11, 385, 399, 1015, 1159, 1683
BstH2I	RGCGCY	1 cut(s)	1339
BstHHI	GCGC	3 cut(s)	1015, 1338, 1685
BstKTI	GATC	7 cut(s)	24, 220, 417, 462, 496, 1038, 1554
BstMAI	GTCTC	4 cut(s)	516, 1087, 1354, 1666
BstMBI	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	5 cut(s)	97, 166, 391, 1169, 1421
BstNI	CCWGG	2 cut(s)	1087, 1712
BstNSI	RCATGY	1 cut(s)	660
BstPAI	GACNNNNGTC	1 cut(s)	714
BstSCI	CCNGG	4 cut(s)	712, 1085, 1220, 1710
BstSLI	GKGCMC	4 cut(s)	93, 293, 707, 1452
BstUI	CGCG	6 cut(s)	11, 385, 399, 1015, 1159, 1683
BstV2I	GAAGAC	2 cut(s)	281, 1724
BstX2I	RGATCY	2 cut(s)	217, 1035
BstYI	RGATCY	2 cut(s)	217, 1035
Bsu15I	ATCGAT	1 cut(s)	462
BsuRI	GGCC	2 cut(s)	301, 1220
BsuTUI	ATCGAT	1 cut(s)	462
BtgI	CCRYGG	1 cut(s)	103
BtgZI	GCGATG	1 cut(s)	1443
BtsCI	GGATG	5 cut(s)	354, 1426, 1582, 1605, 1672
BtsI	GCAGTG	1 cut(s)	1547
BtsIMutI	CAGTG	2 cut(s)	549, 1547
BveI	ACCTGC	1 cut(s)	699
Cac8I	GCNNGC	4 cut(s)	162, 303, 658, 1161
CciI	TCATGA	1 cut(s)	1005
CfoI	GCGC	3 cut(s)	1015, 1338, 1685
Cfr10I	RCCGGY	2 cut(s)	301, 1102
Cfr13I	GGNCC	2 cut(s)	13, 299
ClaI	ATCGAT	1 cut(s)	462
CseI	GACGC	2 cut(s)	829, 881
Csp6I	GTAC	2 cut(s)	474, 584
CviAII	CATG	9 cut(s)	104, 485, 497, 657, 856, 1006, 1296, 1445, 1453
CviQI	GTAC	2 cut(s)	474, 584
DdeI	CTNAG	5 cut(s)	477, 801, 1119, 1164, 1371
DpnI	GATC	7 cut(s)	23, 219, 416, 461, 495, 1037, 1553
DpnII	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
EaeI	YGGCCR	1 cut(s)	1218
Eam1104I	CTCTTC	1 cut(s)	1563
EarI	CTCTTC	1 cut(s)	1563
Ecl136II	GAGCTC	1 cut(s)	503
Eco130I	CCWWGG	2 cut(s)	103, 1024
Eco24I	GRGCYC	1 cut(s)	505
Eco31I	GGTCTC	1 cut(s)	1666
Eco47I	GGWCC	1 cut(s)	13
Eco53kI	GAGCTC	1 cut(s)	503
Eco57I	CTGAAG	4 cut(s)	855, 950, 1203, 1316
Eco88I	CYCGRG	1 cut(s)	729
EcoICRI	GAGCTC	1 cut(s)	503
EcoRI	GAATTC	1 cut(s)	821
EcoRII	CCWGG	2 cut(s)	1085, 1710
EcoT14I	CCWWGG	2 cut(s)	103, 1024
EcoT22I	ATGCAT	1 cut(s)	1210
EcoT38I	GRGCYC	1 cut(s)	505
ErhI	CCWWGG	2 cut(s)	103, 1024
Esp3I	CGTCTC	1 cut(s)	516
FaeI	CATG	9 cut(s)	107, 488, 500, 660, 859, 1009, 1299, 1448, 1456
FatI	CATG	9 cut(s)	103, 484, 496, 656, 855, 1005, 1295, 1444, 1452
FauI	CCCGC	2 cut(s)	378, 1688
FbaI	TGATCA	1 cut(s)	493
FblI	GTMKAC	1 cut(s)	797
Fnu4HI	GCNGC	2 cut(s)	183, 186
FokI	GGATG	5 cut(s)	361, 1433, 1589, 1592, 1679
FriOI	GRGCYC	1 cut(s)	505
Fsp4HI	GCNGC	2 cut(s)	183, 186
FspBI	CTAG	1 cut(s)	1343
GlaI	GCGC	3 cut(s)	1014, 1337, 1684
GluI	GCNGC	2 cut(s)	183, 186
GsuI	CTGGAG	1 cut(s)	1069
HaeII	RGCGCY	1 cut(s)	1339
HaeIII	GGCC	2 cut(s)	301, 1220
HapII	CCGG	4 cut(s)	302, 714, 1103, 1221
HgaI	GACGC	2 cut(s)	829, 881
HhaI	GCGC	3 cut(s)	1015, 1338, 1685
Hin1I	GRCGYC	2 cut(s)	402, 840
Hin1II	CATG	9 cut(s)	107, 488, 500, 660, 859, 1009, 1299, 1448, 1456
Hin6I	GCGC	3 cut(s)	1013, 1336, 1683
HinP1I	GCGC	3 cut(s)	1013, 1336, 1683
HincII	GTYRAC	1 cut(s)	1021
HindII	GTYRAC	1 cut(s)	1021
HindIII	AAGCTT	1 cut(s)	761
HpaII	CCGG	4 cut(s)	302, 714, 1103, 1221
HphI	GGTGA	4 cut(s)	638, 901, 1645, 1726
Hpy166II	GTNNAC	5 cut(s)	291, 705, 798, 1021, 1435
Hpy188III	TCNNGA	6 cut(s)	221, 418, 524, 1006, 1127, 1470
Hpy8I	GTNNAC	5 cut(s)	291, 705, 798, 1021, 1435
Hpy99I	CGWCG	1 cut(s)	404
HpyCH4III	ACNGT	3 cut(s)	79, 583, 1060
HpyCH4IV	ACGT	2 cut(s)	29, 402
HpyF10VI	GCNNNNNNNGC	5 cut(s)	97, 166, 391, 1169, 1421
HpyF3I	CTNAG	5 cut(s)	477, 801, 1119, 1164, 1371
HpySE526I	ACGT	2 cut(s)	29, 402
Hsp92I	GRCGYC	2 cut(s)	402, 840
Hsp92II	CATG	9 cut(s)	107, 488, 500, 660, 859, 1009, 1299, 1448, 1456
HspAI	GCGC	3 cut(s)	1013, 1336, 1683
KroI	GCCGGC	1 cut(s)	301
KroNI	GCCGGC	1 cut(s)	303
Ksp22I	TGATCA	1 cut(s)	493
Kzo9I	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
LmnI	GCTCC	2 cut(s)	500, 508
LweI	GCATC	3 cut(s)	1195, 1411, 1466
MaeI	CTAG	1 cut(s)	1343
MaeII	ACGT	2 cut(s)	29, 402
MaeIII	GTNAC	3 cut(s)	374, 1467, 1669
MalI	GATC	7 cut(s)	23, 219, 416, 461, 495, 1037, 1553
MboI	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
MboII	GAAGA	8 cut(s)	286, 303, 424, 907, 989, 1259, 1550, 1729
MfeI	CAATTG	2 cut(s)	294, 1233
MflI	RGATCY	2 cut(s)	217, 1035
MhlI	GDGCHC	5 cut(s)	93, 293, 505, 707, 1452
MluI	ACGCGT	1 cut(s)	1157
MlyI	GAGTC	4 cut(s)	1139, 1309, 1356, 1595
MmeI	TCCRAC	3 cut(s)	213, 1079, 1178
Mph1103I	ATGCAT	1 cut(s)	1210
MroNI	GCCGGC	1 cut(s)	301
MroXI	GAANNNNTTC	3 cut(s)	963, 1050, 1188
MseI	TTAA	4 cut(s)	207, 516, 1094, 1566
MslI	CAYNNNNRTG	1 cut(s)	1523
MspA1I	CMGCKG	1 cut(s)	1391
MspI	CCGG	4 cut(s)	302, 714, 1103, 1221
MspR9I	CCNGG	4 cut(s)	714, 1087, 1222, 1712
MunI	CAATTG	2 cut(s)	294, 1233
Mva1269I	GAATGC	1 cut(s)	1697
MvaI	CCWGG	2 cut(s)	1087, 1712
MvnI	CGCG	6 cut(s)	11, 385, 399, 1015, 1159, 1683
MwoI	GCNNNNNNNGC	5 cut(s)	97, 166, 391, 1169, 1421
NaeI	GCCGGC	1 cut(s)	303
NciI	CCSGG	2 cut(s)	714, 1222
NcoI	CCATGG	1 cut(s)	103
NdeII	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
NgoMIV	GCCGGC	1 cut(s)	301
NlaIII	CATG	9 cut(s)	107, 488, 500, 660, 859, 1009, 1299, 1448, 1456
NlaIV	GGNNCC	1 cut(s)	395
NmuCI	GTSAC	3 cut(s)	374, 1467, 1669
NsiI	ATGCAT	1 cut(s)	1210
NspI	RCATGY	1 cut(s)	660
PaeI	GCATGC	1 cut(s)	660
PaeR7I	CTCGAG	1 cut(s)	729
PagI	TCATGA	1 cut(s)	1005
PcsI	WCGNNNNNNNCGW	1 cut(s)	26
PctI	GAATGC	1 cut(s)	1697
PdiI	GCCGGC	1 cut(s)	303
PdmI	GAANNNNTTC	3 cut(s)	963, 1050, 1188
PfeI	GAWTC	7 cut(s)	481, 530, 828, 878, 1002, 1051, 1226
PkrI	GCNGC	2 cut(s)	184, 187
PleI	GAGTC	4 cut(s)	1138, 1308, 1355, 1594
PpsI	GAGTC	4 cut(s)	1138, 1308, 1355, 1594
Ppu21I	YACGTR	1 cut(s)	30
PshAI	GACNNNNGTC	1 cut(s)	714
PsiI	TTATAA	1 cut(s)	986
Psp124BI	GAGCTC	1 cut(s)	505
Psp6I	CCWGG	2 cut(s)	1085, 1710
PspGI	CCWGG	2 cut(s)	1085, 1710
PspN4I	GGNNCC	1 cut(s)	395
PspPI	GGNCC	2 cut(s)	13, 299
PspXI	VCTCGAGB	1 cut(s)	729
PsuI	RGATCY	2 cut(s)	217, 1035
PvuII	CAGCTG	1 cut(s)	1391
RsaI	GTAC	2 cut(s)	475, 585
RsaNI	GTAC	2 cut(s)	474, 584
RseI	CAYNNNNRTG	1 cut(s)	1523
SacI	GAGCTC	1 cut(s)	505
SaqAI	TTAA	4 cut(s)	207, 516, 1094, 1566
SatI	GCNGC	2 cut(s)	183, 186
Sau3AI	GATC	7 cut(s)	21, 217, 414, 459, 493, 1035, 1551
Sau96I	GGNCC	2 cut(s)	13, 299
SchI	GAGTC	4 cut(s)	1139, 1309, 1356, 1595
ScrFI	CCNGG	4 cut(s)	714, 1087, 1222, 1712
SduI	GDGCHC	5 cut(s)	93, 293, 505, 707, 1452
SfaNI	GCATC	3 cut(s)	1195, 1411, 1466
Sfr274I	CTCGAG	1 cut(s)	729
SinI	GGWCC	1 cut(s)	13
SlaI	CTCGAG	1 cut(s)	729
SmiMI	CAYNNNNRTG	1 cut(s)	1523
SmlI	CTYRAG	3 cut(s)	729, 1127, 1597
SmoI	CTYRAG	3 cut(s)	729, 1127, 1597
SphI	GCATGC	1 cut(s)	660
SsiI	CCGC	9 cut(s)	5, 11, 164, 182, 185, 385, 397, 1363, 1681
SspMI	CTAG	1 cut(s)	1343
SstI	GAGCTC	1 cut(s)	505
StyD4I	CCNGG	4 cut(s)	712, 1085, 1220, 1710
StyI	CCWWGG	2 cut(s)	103, 1024
TaaI	ACNGT	3 cut(s)	79, 583, 1060
TaiI	ACGT	2 cut(s)	32, 405
TaqI	TCGA	4 cut(s)	20, 462, 730, 1192
TatI	WGTACW	1 cut(s)	583
TauI	GCSGC	2 cut(s)	185, 188
TfiI	GAWTC	7 cut(s)	481, 530, 828, 878, 1002, 1051, 1226
Tru1I	TTAA	4 cut(s)	207, 516, 1094, 1566
Tru9I	TTAA	4 cut(s)	207, 516, 1094, 1566
TscAI	CASTG	2 cut(s)	556, 1547
TseFI	GTSAC	3 cut(s)	374, 1467, 1669
Tsp45I	GTSAC	3 cut(s)	374, 1467, 1669
TspDTI	ATGAA	4 cut(s)	365, 473, 612, 1527
TspRI	CASTG	2 cut(s)	556, 1547
VneI	GTGCAC	2 cut(s)	289, 703
VpaK11BI	GGWCC	1 cut(s)	13
XapI	RAATTY	5 cut(s)	557, 737, 821, 970, 1534
XceI	RCATGY	1 cut(s)	660
XcmI	CCANNNNNNNNNTGG	3 cut(s)	32, 934, 1285
XhoI	CTCGAG	1 cut(s)	729
XmiI	GTMKAC	1 cut(s)	797
XmnI	GAANNNNTTC	3 cut(s)	963, 1050, 1188
XspI	CTAG	1 cut(s)	1343
ZraI	GACGTC	1 cut(s)	403
Zsp2I	ATGCAT	1 cut(s)	1210

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.