Rh6AG044700

Belongs to the pyruvate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0006384
Physical Location & Seq
Forward (+)
6524025 .. 6529475
5451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG044700.1

Sequence Viewer

Length: 1446 bp
ATGGCTGTCTCATCTAGCCATTCGATTCGTACGTGTTTTCAGATCAAAGCCAAGAGCCCGGGTTTGAATCTGGAGAAGAGGGTCTTTGGATTACCAGTTTTGCAACATGGGGTTGCTTGTGTTGGGAAGAAACTCAAGGCTAAAGTTGGGACTAAAGTTGAGGCAGTACAGGTGGGTTTGGAGGGGACAGAGACTTTGACTGATCTCAAGGGTTTAGGGAGGGCTTTGGAGCTTGATGTGGTGTCAGAGAGGGAGCTGAGGGAGAAGGGTTTCTTGGGGCTGAGGAAGACCAAGCTTGTGTGCACGATCGGTCCGGCGTGTGATTCTTTGGAGGATTTGGAGAAATTGGTGTTGGGTGGGATGAATGTGGCAAGGCTCAATATGTGTCACAACACTAGGGAGTGGCACCGGGATGTGATTCGGAAGATTAAGAAGTTGAATGAGGAAAAGGGGTATTCTGTTTCGATTATGATTGATACGGAAGGGAGTCAGATTCATGTTGTTGATCATGGAGAGCCGACCTCTCTCAAAGTTCAGGAGGATTCAATCTGGCTATTTACCCCTGAAAAATTTGAGGGTTCTGTTCCATTCACAGTTCAAGCAAACTACGAAGGTTTTTCTGAAGGTATTAAAGTGGGTGATGAACTTGTTATTGATGGTGGAATGGCACGCTTTGAAGTCATTGAAAGGCTTGGAAGTGATCTACGTTGTAAGTGCATAGACTCCGGTTTGTTCCTGCCTTGTGCAAAGTTCAGCTTTTGGAGAGATGGGAAACTGGTGGAGAGGAACTATGAGCTTCCTACTCTGTCAACAAAGGATTGGTCTGACATCGAATTTGGAATTTCTGAAGGTATTGATTTCATTGCCATGTCCTTCGTAAATGATGCTGAATCTGTCAGGCATTTGAAAGATTACCTCACTATCAAATCACTCAAATCCGTTAGAGTTTTGGCAAAAATTGAGAGCTTGGAAGCTCTTCAGAAGCTGGAAGAAATCATTGAAGCTTCTGATGGAGTCATGATTGCTCGGGTTGATCTTGGAGTTGAAATCCCACTCGAGCAGATTCCAACAGTCCAAGAGGAAATAACTCAAGTGTGTAGGCAGCTAAACAAGCCAGTAATTGTAGCTTCTCAACTACTTGAATCCATGATTGAATACCCAACCCCAACACGCGCTGAGGTTGCAGATGTCTCTGAAGCTGTTCGACAGTATGCAGATGCCTTGATGCTAGCTGGGGAATCAGCCATTGGATTATATGGGCAGAAGGCTCTATCTGTCCTTCAAATGACCAGCAGTAGAATGGAGGCATGGAGTCGTGTAGAAAACCGCCAAGATCTTCTGCAGCTACGGCAACTAGGAGTCTCATTGCCTGATCGCATTGCTGAGCAGATATGCAATTCTGCTGTCGGAATGGGTACTTTTCTTCCATTCCTCTTTTATAAGTGA

Protein Analysis

481

Amino Acids

53.46

Weight (kDa)

5.41

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 95 - 423 1.7e-82 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1440
AccB1I GGYRCC 1 cut(s) 405
AccII CGCG 1 cut(s) 1173
AciI CCGC 1 cut(s) 1327
AcsI RAATTY 3 cut(s) 569, 833, 840
AcuI CTGAAG 4 cut(s) 642, 867, 962, 1215
AfaI GTAC 3 cut(s) 31, 168, 1417
AflIII ACRYGT 1 cut(s) 32
AjuI GAANNNNNNNTTGG 6 cut(s) 257, 289, 335, 367, 1230, 1262
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 4 cut(s) 13, 185, 1195, 1366
Alw44I GTGCAC 1 cut(s) 301
AlwNI CAGNNNCTG 1 cut(s) 985
Ama87I CYCGRG 3 cut(s) 58, 1026, 1055
ApaLI GTGCAC 1 cut(s) 301
ApeKI GCWGC 2 cut(s) 1102, 1342
ApoI RAATTY 3 cut(s) 569, 833, 840
ArsI GACNNNNNNTTYG 6 cut(s) 178, 210, 806, 818, 838, 850
Asp700I GAANNNNTTC 3 cut(s) 269, 975, 1200
AspLEI GCGC 1 cut(s) 1175
AspS9I GGNCC 1 cut(s) 311
AsuC2I CCSGG 3 cut(s) 59, 60, 410
AsuHPI GGTGA 1 cut(s) 650
AsuNHI GCTAGC 1 cut(s) 1228
AvaI CYCGRG 3 cut(s) 58, 1026, 1055
AvaII GGWCC 1 cut(s) 311
BaeGI GKGCMC 1 cut(s) 305
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 305
BbvCI CCTCAGC 3 cut(s) 257, 281, 1176
BbvI GCAGC 2 cut(s) 1114, 1354
BccI CCATC 3 cut(s) 650, 761, 1004
BceAI ACGGC 1 cut(s) 1364
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 3 cut(s) 59, 60, 410
BcoDI GTCTC 4 cut(s) 13, 185, 1195, 1366
BfaI CTAG 4 cut(s) 15, 396, 1229, 1355
BfmI CTRYAG 1 cut(s) 1340
BglII AGATCT 1 cut(s) 1333
BisI GCNGC 2 cut(s) 1103, 1343
BlpI GCTNAGC 1 cut(s) 1383
BlsI GCNGC 2 cut(s) 1104, 1344
Bme1390I CCNGG 3 cut(s) 59, 60, 410
Bme18I GGWCC 1 cut(s) 311
BmeT110I CYCGRG 3 cut(s) 58, 1026, 1055
BmgT120I GGNCC 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 407
BmrFI CCNGG 3 cut(s) 59, 60, 410
BmsI GCATC 3 cut(s) 874, 1207, 1215
BmtI GCTAGC 1 cut(s) 1232
BpiI GAAGAC 1 cut(s) 293
BplI GAGNNNNNCTC 2 cut(s) 506, 538
BpmI CTGGAG 1 cut(s) 92
Bpu10I CCTNAGC 3 cut(s) 257, 281, 1176
Bpu1102I GCTNAGC 1 cut(s) 1383
BpuEI CTTGAG 3 cut(s) 119, 191, 1074
BpuMI CCSGG 3 cut(s) 59, 60, 410
BsaAI YACGTR 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 58
BsaWI WCCGGW 1 cut(s) 725
Bse1I ACTGG 3 cut(s) 95, 780, 1115
Bse3DI GCAATG 3 cut(s) 861, 1364, 1377
BseDI CCNNGG 1 cut(s) 58
BseGI GGATG 2 cut(s) 366, 418
BseMI GCAATG 3 cut(s) 861, 1364, 1377
BseMII CTCAG 4 cut(s) 248, 272, 1167, 1374
BseNI ACTGG 3 cut(s) 95, 780, 1115
BseSI GKGCMC 1 cut(s) 305
BseXI GCAGC 2 cut(s) 1114, 1354
BseYI CCCAGC 1 cut(s) 1232
Bsh1236I CGCG 1 cut(s) 1173
Bsh1285I CGRYCG 1 cut(s) 309
BshNI GGYRCC 1 cut(s) 405
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 305
BsiHKCI CYCGRG 3 cut(s) 58, 1026, 1055
BsiSI CCGG 4 cut(s) 59, 314, 409, 726
BsiWI CGTACG 1 cut(s) 29
BslFI GGGAC 2 cut(s) 163, 199
BsmAI GTCTC 4 cut(s) 13, 185, 1195, 1366
BsmFI GGGAC 2 cut(s) 163, 199
BsoBI CYCGRG 3 cut(s) 58, 1026, 1055
Bsp1286I GDGCHC 2 cut(s) 59, 305
Bsp143I GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
Bsp1720I GCTNAGC 1 cut(s) 1383
BspACI CCGC 1 cut(s) 1327
BspCNI CTCAG 4 cut(s) 249, 273, 1168, 1375
BspFNI CGCG 1 cut(s) 1173
BspHI TCATGA 1 cut(s) 1017
BspLI GGNNCC 1 cut(s) 407
BspMAI CTGCAG 1 cut(s) 1344
BspOI GCTAGC 1 cut(s) 1232
BspQI GCTCTTC 1 cut(s) 981
BspT107I GGYRCC 1 cut(s) 405
BsrDI GCAATG 3 cut(s) 861, 1364, 1377
BsrI ACTGG 3 cut(s) 95, 780, 1115
BssECI CCNNGG 1 cut(s) 58
BssMI GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
Bst4CI ACNGT 3 cut(s) 595, 1072, 1209
Bst6I CTCTTC 2 cut(s) 71, 981
BstBAI YACGTR 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 670, 1230
BstDEI CTNAG 4 cut(s) 257, 281, 1176, 1383
BstF5I GGATG 2 cut(s) 366, 418
BstFNI CGCG 1 cut(s) 1173
BstHHI GCGC 1 cut(s) 1175
BstKTI GATC 8 cut(s) 45, 205, 309, 508, 703, 1036, 1336, 1375
BstMAI GTCTC 4 cut(s) 13, 185, 1195, 1366
BstMBI GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 3 cut(s) 1111, 1181, 1348
BstSCI CCNGG 3 cut(s) 57, 58, 408
BstSFI CTRYAG 1 cut(s) 1340
BstSLI GKGCMC 1 cut(s) 305
BstUI CGCG 1 cut(s) 1173
BstV1I GCAGC 2 cut(s) 1114, 1354
BstV2I GAAGAC 1 cut(s) 293
BstX2I RGATCY 1 cut(s) 1333
BstYI RGATCY 1 cut(s) 1333
BtsCI GGATG 2 cut(s) 366, 418
Cac8I GCNNGC 2 cut(s) 670, 1230
CaiI CAGNNNCTG 1 cut(s) 985
CciI TCATGA 1 cut(s) 1017
CfoI GCGC 1 cut(s) 1175
Cfr13I GGNCC 1 cut(s) 311
Cfr9I CCCGGG 1 cut(s) 58
CpoI CGGWCCG 1 cut(s) 311
Csp6I GTAC 3 cut(s) 30, 167, 1416
CspI CGGWCCG 1 cut(s) 311
CviAII CATG 7 cut(s) 107, 497, 509, 868, 1018, 1147, 1308
CviQI GTAC 3 cut(s) 30, 167, 1416
DdeI CTNAG 4 cut(s) 257, 281, 1176, 1383
DpnI GATC 8 cut(s) 44, 204, 308, 507, 702, 1035, 1335, 1374
DpnII GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
Eam1104I CTCTTC 2 cut(s) 71, 981
EarI CTCTTC 2 cut(s) 71, 981
Eco24I GRGCYC 1 cut(s) 59
Eco47I GGWCC 1 cut(s) 311
Eco57I CTGAAG 4 cut(s) 642, 867, 962, 1215
Eco88I CYCGRG 3 cut(s) 58, 1026, 1055
EcoT38I GRGCYC 1 cut(s) 59
FaeI CATG 7 cut(s) 110, 500, 512, 871, 1021, 1150, 1311
FalI AAGNNNNNCTT 6 cut(s) 68, 100, 257, 289, 740, 772
FaqI GGGAC 2 cut(s) 163, 199
FatI CATG 7 cut(s) 106, 496, 508, 867, 1017, 1146, 1307
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 2 cut(s) 1103, 1343
FokI GGATG 2 cut(s) 373, 425
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 2 cut(s) 1103, 1343
FspBI CTAG 4 cut(s) 15, 396, 1229, 1355
GlaI GCGC 1 cut(s) 1174
GluI GCNGC 2 cut(s) 1103, 1343
GsaI CCCAGC 1 cut(s) 1236
GsuI CTGGAG 1 cut(s) 92
HapII CCGG 4 cut(s) 59, 314, 409, 726
HhaI GCGC 1 cut(s) 1175
Hin1II CATG 7 cut(s) 110, 500, 512, 871, 1021, 1150, 1311
Hin6I GCGC 1 cut(s) 1173
HinP1I GCGC 1 cut(s) 1173
HincII GTYRAC 1 cut(s) 810
HindII GTYRAC 1 cut(s) 810
HindIII AAGCTT 2 cut(s) 293, 1002
HpaII CCGG 4 cut(s) 59, 314, 409, 726
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 2 cut(s) 303, 810
Hpy188III TCNNGA 3 cut(s) 71, 536, 1018
Hpy8I GTNNAC 2 cut(s) 303, 810
HpyAV CCTTC 8 cut(s) 259, 476, 605, 617, 842, 883, 1258, 1289
HpyCH4III ACNGT 3 cut(s) 595, 1072, 1209
HpyCH4IV ACGT 2 cut(s) 32, 706
HpyCH4V TGCA 8 cut(s) 103, 303, 717, 746, 1184, 1214, 1342, 1395
HpyF10VI GCNNNNNNNGC 3 cut(s) 1111, 1181, 1348
HpyF3I CTNAG 4 cut(s) 257, 281, 1176, 1383
HpySE526I ACGT 2 cut(s) 32, 706
Hsp92II CATG 7 cut(s) 110, 500, 512, 871, 1021, 1150, 1311
HspAI GCGC 1 cut(s) 1173
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
LguI GCTCTTC 1 cut(s) 981
LmnI GCTCC 2 cut(s) 229, 253
Lsp1109I GCAGC 2 cut(s) 1114, 1354
LweI GCATC 3 cut(s) 874, 1207, 1215
MaeI CTAG 4 cut(s) 15, 396, 1229, 1355
MaeII ACGT 2 cut(s) 32, 706
MaeIII GTNAC 1 cut(s) 386
MalI GATC 8 cut(s) 44, 204, 308, 507, 702, 1035, 1335, 1374
MboI GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
MboII GAAGA 8 cut(s) 88, 139, 298, 436, 968, 1001, 1328, 1415
MflI RGATCY 1 cut(s) 1333
MhlI GDGCHC 2 cut(s) 59, 305
MluCI AATT 7 cut(s) 344, 569, 833, 840, 957, 1119, 1396
MlyI GAGTC 5 cut(s) 496, 716, 1023, 1321, 1368
MmeI TCCRAC 2 cut(s) 1091, 1387
MroXI GAANNNNTTC 3 cut(s) 269, 975, 1200
MseI TTAA 2 cut(s) 429, 630
MslI CAYNNNNRTG 2 cut(s) 411, 866
MspI CCGG 4 cut(s) 59, 314, 409, 726
MspR9I CCNGG 3 cut(s) 59, 60, 410
MvnI CGCG 1 cut(s) 1173
MwoI GCNNNNNNNGC 3 cut(s) 1111, 1181, 1348
NciI CCSGG 3 cut(s) 59, 60, 410
NdeII GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
NheI GCTAGC 1 cut(s) 1228
NlaIII CATG 7 cut(s) 110, 500, 512, 871, 1021, 1150, 1311
NlaIV GGNNCC 1 cut(s) 407
NmuCI GTSAC 1 cut(s) 386
PaeR7I CTCGAG 1 cut(s) 1055
PagI TCATGA 1 cut(s) 1017
PciSI GCTCTTC 1 cut(s) 981
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PdmI GAANNNNTTC 3 cut(s) 269, 975, 1200
Pfl23II CGTACG 1 cut(s) 29
PkrI GCNGC 2 cut(s) 1104, 1344
Ple19I CGATCG 1 cut(s) 309
PleI GAGTC 5 cut(s) 495, 716, 1022, 1320, 1367
PpsI GAGTC 5 cut(s) 495, 716, 1022, 1320, 1367
Ppu21I YACGTR 1 cut(s) 33
PsiI TTATAA 1 cut(s) 1440
PspFI CCCAGC 1 cut(s) 1232
PspLI CGTACG 1 cut(s) 29
PspN4I GGNNCC 1 cut(s) 407
PspPI GGNCC 1 cut(s) 311
PspXI VCTCGAGB 1 cut(s) 1055
PstI CTGCAG 1 cut(s) 1344
PstNI CAGNNNCTG 1 cut(s) 985
PsuI RGATCY 1 cut(s) 1333
PvuI CGATCG 1 cut(s) 309
RsaI GTAC 3 cut(s) 31, 168, 1417
RsaNI GTAC 3 cut(s) 30, 167, 1416
RseI CAYNNNNRTG 2 cut(s) 411, 866
Rsr2I CGGWCCG 1 cut(s) 311
RsrII CGGWCCG 1 cut(s) 311
SapI GCTCTTC 1 cut(s) 981
SaqAI TTAA 2 cut(s) 429, 630
SatI GCNGC 2 cut(s) 1103, 1343
Sau3AI GATC 8 cut(s) 42, 202, 306, 505, 700, 1033, 1333, 1372
Sau96I GGNCC 1 cut(s) 311
SchI GAGTC 5 cut(s) 496, 716, 1023, 1321, 1368
ScrFI CCNGG 3 cut(s) 59, 60, 410
SduI GDGCHC 2 cut(s) 59, 305
SfaNI GCATC 3 cut(s) 874, 1207, 1215
SfcI CTRYAG 1 cut(s) 1340
Sfr274I CTCGAG 1 cut(s) 1055
SinI GGWCC 1 cut(s) 311
SlaI CTCGAG 1 cut(s) 1055
SmaI CCCGGG 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 411, 866
SmlI CTYRAG 4 cut(s) 134, 206, 1055, 1089
SmoI CTYRAG 4 cut(s) 134, 206, 1055, 1089
Sse9I AATT 7 cut(s) 344, 569, 833, 840, 957, 1119, 1396
SsiI CCGC 1 cut(s) 1327
SspMI CTAG 4 cut(s) 15, 396, 1229, 1355
StyD4I CCNGG 3 cut(s) 57, 58, 408
TaaI ACNGT 3 cut(s) 595, 1072, 1209
TaiI ACGT 2 cut(s) 35, 709
TaqI TCGA 5 cut(s) 23, 464, 831, 1056, 1204
TaqII GACCGA 1 cut(s) 299
TasI AATT 7 cut(s) 344, 569, 833, 840, 957, 1119, 1396
TatI WGTACW 1 cut(s) 166
Tru1I TTAA 2 cut(s) 429, 630
Tru9I TTAA 2 cut(s) 429, 630
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 2 cut(s) 1102, 1342
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 4 cut(s) 377, 485, 657, 850
TspGWI ACGGA 2 cut(s) 494, 928
TspMI CCCGGG 1 cut(s) 58
VneI GTGCAC 1 cut(s) 301
VpaK11BI GGWCC 1 cut(s) 311
XapI RAATTY 3 cut(s) 569, 833, 840
XcmI CCANNNNNNNNNTGG 1 cut(s) 1297
XhoI CTCGAG 1 cut(s) 1055
XmaI CCCGGG 1 cut(s) 58
XmnI GAANNNNTTC 3 cut(s) 269, 975, 1200
XspI CTAG 4 cut(s) 15, 396, 1229, 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.