Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008839

Physical Location & Seq

Genomic Coordinates Reverse (-)

32109198 .. 32113859

Gene Structure 4662 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1193300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1470 bp

ATGGCGGTGGTCGGCCTCTACCGCCGGGAGCTTCCCTCTCCTCCTGCAATCGAATTTGCTTCCCCAGACGGAAAGAAGTTATTTACTGAAGCTCTTGGAAATGGAACCATGGAGGGGTTTTTCAAGTTGATCTCTTACTACCAAACGCAGTCGGAGCCTGCTTATTGCGGGCTGGCCACCCTCGCCGTCGTCCTCAACGCCCTCGCCATTGATCCCGGAAGAAAATGGAAAGGCCCATGGAGATGGTTTGATGACTCAATGCTAGATTGCTGTGAGCCTTTGGCAAAGGTCAAAGCCGAAGGCATCACATTCGGGAAAGTTGCATGCTTGGCGCATTGCAACGGAGCTGAAGTGAAGGCATTACGAACCGAAGAAAGTTCGGTGGATGAATTTCGTAAGCATGTCATTTCATGTACTTCTTCAGAGGATTGTCATGTCATCACATCTTACCACAGAGCTGCATTTAGCCAGACTGGAAGTGGTCATTTTTCACCGATTGGTGGCTATCACGCCGGAACAGATATGGTTCTCATTTTGGATGTTGCTCGATTCAAATATCCTCCTCATTGGGTTCCCCTTTCGCTTCTCTGGGAGGGAATGACTACTATTGATGAAGCAACTGGACATTGTCGGGGGTACATGATCATTTCAAGGCTTAACAGAAGCCCGTCTATTCTCTACACTGTGAGTTGCAGACATGAAGGTTGGAGTAGTATTGCGAAGTACTTAACAGAAGACGTTCCTCTCTTGTTGAAGTCGGAAGATATAAAAGATGTTCAGAATGTAATCTCTGTTTTCTTCAAATCACCACCTGCTGACTTGAGAGAGTTCATTAAATGGGTTGCTGAAGTTCGGAGACAAGAGGATGGGAATCTGAGCTTAGACAAAGAGGAAAAAGGAAGGCTCGCTATCAAGGAAGAGGTATTAAAACAAGTAAGAGAAACGGAACTCTTCAGACACGTAAGCGAATGGTTGGCTTCTAAATCAATCTGTAAAGACATATCACAAGGTGGTAAAGATACATTGCCTGAAATTGCTGCCAATGTTTGTTGCCAAGGAGGACAAGTATTTTCCGGGAGTGTCAGTTCATTTGATCGAGCATGCTGTAGGCAAACTGACGTGAAACTCTTGAAGTGCAATGGTGAAAAGCCCGTTACCGTGGTGTCGGGGACTGTAACAACAAATGGCGGTGAACAAGCAGTTGAGATGCTGGTTCCCTCATGTCAAACCGAACCAAGCAGCTTGTGTTCTTATTTTCAAGGTAGCTGCATAGGGATGCATCCATCTACAGGTGATGTTCTAACAGTACTTCTATTGGCTCTGCCTCAACAAACATGGTCTGGTCTTAGAGAAGCGAAGCTACTGGCAGAAATCAATTGCCTCATATCAACCGATTGTCTACCAACTTTGCTTCAAGAGGAGGTTTTCTACTTGCGACGGCAAATCCACTTCCTCATGACTGATAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Phytochelatin Phytochelatin_C

Protein Analysis

490

Amino Acids

54.17

Weight (kDa)

5.9

Isoelectric Point (pI)

51.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phytochelatin	PF05023	7 - 213	8.8e-91	Phytochelatin synthase
Phytochelatin_C	PF09328	231 - 482	3.5e-114	Domain of unknown function (DUF1984)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008839)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g21020 FvH4_2g21020 FvH4_2g21020 FvH4_2g21050 FvH4_2g21050 FvH4_2g21050 FvH4_2g21050 FvH4_2g21050
malus_domestica	MD05G1193300.v1.1
prunus_persica	Prupe.8G219800_v2.0.a1
pyrus_communis	pycom05g18040
rosa_chinensis	RchiOBHm_Chr6g0287501
rosa_laevigata	RLG00000012489
rosa_multiflora	Rmu_sc0007029.1_g000013
rosa_roxburghii	Rroxscaffold_7G00181420
rosa_rugosa	Rorug06G0188400.1 Rorug06G0188500.1
rosa_samantha	Rh6AG299800 Rh6BG305200 Rh6CG304300 Rh6CG313600 Rh6DG297000
rosa_wichuraiana	Rw6G025860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	820
Acc36I	ACCTGC	1 cut(s)	820
AccI	GTMKAC	1 cut(s)	1399
AciI	CCGC	4 cut(s)	5, 22, 168, 1188
AclWI	GGATC	1 cut(s)	206
AcoI	YGGCCR	1 cut(s)	174
AcsI	RAATTY	2 cut(s)	53, 389
AcuI	CTGAAG	5 cut(s)	108, 369, 405, 867, 937
AdeI	CACNNNGTG	1 cut(s)	1010
AfaI	GTAC	4 cut(s)	415, 638, 725, 1308
AfiI	CCNNNNNNNGG	3 cut(s)	114, 500, 1289
AflIII	ACRYGT	1 cut(s)	958
AgsI	TTSAA	8 cut(s)	124, 553, 651, 754, 802, 1132, 1259, 1415
AjiI	CACGTC	1 cut(s)	1120
AloI	GAACNNNNNNTCC	2 cut(s)	1069, 1101
AluBI	AGCT	8 cut(s)	31, 92, 347, 458, 879, 1242, 1266, 1360
AluI	AGCT	8 cut(s)	31, 92, 347, 458, 879, 1242, 1266, 1360
Alw26I	GTCTC	1 cut(s)	850
AlwI	GGATC	1 cut(s)	206
AoxI	GGCC	3 cut(s)	13, 174, 232
ApeKI	GCWGC	4 cut(s)	458, 1037, 1239, 1266
ApoI	RAATTY	2 cut(s)	53, 389
Asp700I	GAANNNNTTC	1 cut(s)	738
AspLEI	GCGC	1 cut(s)	334
AspS9I	GGNCC	1 cut(s)	233
AsuC2I	CCSGG	3 cut(s)	26, 216, 1075
AsuHPI	GGTGA	5 cut(s)	483, 798, 1154, 1202, 1304
BalI	TGGCCA	1 cut(s)	176
BarI	GAAGNNNNNNTAC	2 cut(s)	1344, 1376
BbsI	GAAGAC	1 cut(s)	741
BbvI	GCAGC	4 cut(s)	445, 1024, 1251, 1253
BccI	CCATC	3 cut(s)	237, 860, 1291
BceAI	ACGGC	2 cut(s)	170, 1454
BcgI	CGANNNNNNTGC	2 cut(s)	292, 326
BclI	TGATCA	1 cut(s)	642
BcnI	CCSGG	3 cut(s)	26, 216, 1075
BcoDI	GTCTC	1 cut(s)	850
BfaI	CTAG	1 cut(s)	263
BfmI	CTRYAG	2 cut(s)	1105, 1287
BfuAI	ACCTGC	1 cut(s)	820
BisI	GCNGC	4 cut(s)	459, 1038, 1240, 1267
BlsI	GCNGC	4 cut(s)	460, 1039, 1241, 1268
BmcAI	AGTACT	2 cut(s)	725, 1308
Bme1390I	CCNGG	3 cut(s)	26, 216, 1075
BmgBI	CACGTC	1 cut(s)	1120
BmgT120I	GGNCC	1 cut(s)	233
BmiI	GGNNCC	4 cut(s)	106, 156, 573, 1215
BmrFI	CCNGG	3 cut(s)	26, 216, 1075
BmsI	GCATC	4 cut(s)	312, 1197, 1266, 1288
BpiI	GAAGAC	1 cut(s)	741
BplI	GAGNNNNNCTC	2 cut(s)	20, 52
BpuEI	CTTGAG	1 cut(s)	841
BpuMI	CCSGG	3 cut(s)	26, 216, 1075
BsaAI	YACGTR	1 cut(s)	961
BsaBI	GATNNNNATC	1 cut(s)	870
BsaJI	CCNNGG	4 cut(s)	108, 236, 1054, 1158
BsaXI	ACNNNNNCTCC	6 cut(s)	336, 366, 1050, 1069, 1080, 1099
Bsc4I	CCNNNNNNNGG	3 cut(s)	114, 500, 1289
Bse1I	ACTGG	3 cut(s)	478, 625, 1368
Bse3DI	GCAATG	3 cut(s)	334, 1022, 1144
Bse8I	GATNNNNATC	1 cut(s)	870
BseDI	CCNNGG	4 cut(s)	108, 236, 1054, 1158
BseGI	GGATG	5 cut(s)	391, 544, 871, 1279, 1281
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	3 cut(s)	114, 500, 1289
BseMI	GCAATG	3 cut(s)	334, 1022, 1144
BseMII	CTCAG	1 cut(s)	866
BseNI	ACTGG	3 cut(s)	478, 625, 1368
BseRI	GAGGAG	3 cut(s)	30, 552, 1433
BseXI	GCAGC	4 cut(s)	445, 1024, 1251, 1253
BshFI	GGCC	3 cut(s)	15, 176, 234
BsiSI	CCGG	4 cut(s)	25, 216, 513, 1074
BslFI	GGGAC	1 cut(s)	1183
BslI	CCNNNNNNNGG	3 cut(s)	114, 500, 1289
BsmAI	GTCTC	1 cut(s)	850
BsmFI	GGGAC	1 cut(s)	1183
BsnI	GGCC	3 cut(s)	15, 176, 234
Bsp143I	GATC	4 cut(s)	129, 211, 642, 1093
Bsp19I	CCATGG	2 cut(s)	108, 236
BspACI	CCGC	4 cut(s)	5, 22, 168, 1188
BspANI	GGCC	3 cut(s)	15, 176, 234
BspCNI	CTCAG	1 cut(s)	867
BspHI	TCATGA	1 cut(s)	1455
BspLI	GGNNCC	4 cut(s)	106, 156, 573, 1215
BspMI	ACCTGC	1 cut(s)	820
BspPI	GGATC	1 cut(s)	206
BsrDI	GCAATG	3 cut(s)	334, 1022, 1144
BsrI	ACTGG	3 cut(s)	478, 625, 1368
BssECI	CCNNGG	4 cut(s)	108, 236, 1054, 1158
BssMI	GATC	4 cut(s)	129, 211, 642, 1093
BssT1I	CCWWGG	3 cut(s)	108, 236, 1054
Bst4CI	ACNGT	4 cut(s)	685, 1159, 1174, 1306
Bst6I	CTCTTC	2 cut(s)	912, 956
BstBAI	YACGTR	1 cut(s)	961
BstC8I	GCNNGC	6 cut(s)	159, 170, 174, 325, 906, 1102
BstDEI	CTNAG	3 cut(s)	875, 880, 1346
BstDSI	CCRYGG	3 cut(s)	108, 236, 1158
BstF5I	GGATG	5 cut(s)	391, 544, 871, 1279, 1281
BstHHI	GCGC	1 cut(s)	334
BstKTI	GATC	4 cut(s)	132, 214, 645, 1096
BstMAI	GTCTC	1 cut(s)	850
BstMBI	GATC	4 cut(s)	129, 211, 642, 1093
BstMWI	GCNNNNNNNGC	4 cut(s)	21, 154, 182, 329
BstNSI	RCATGY	3 cut(s)	327, 404, 1104
BstSCI	CCNGG	3 cut(s)	24, 214, 1073
BstSFI	CTRYAG	2 cut(s)	1105, 1287
BstV1I	GCAGC	4 cut(s)	445, 1024, 1251, 1253
BstV2I	GAAGAC	1 cut(s)	741
BstXI	CCANNNNNNTGG	1 cut(s)	243
BsuRI	GGCC	3 cut(s)	15, 176, 234
BtgI	CCRYGG	3 cut(s)	108, 236, 1158
BtrI	CACGTC	1 cut(s)	1120
BtsCI	GGATG	5 cut(s)	391, 544, 871, 1279, 1281
BtsIMutI	CAGTG	1 cut(s)	681
BveI	ACCTGC	1 cut(s)	820
Cac8I	GCNNGC	6 cut(s)	159, 170, 174, 325, 906, 1102
CciI	TCATGA	1 cut(s)	1455
CfoI	GCGC	1 cut(s)	334
Cfr13I	GGNCC	1 cut(s)	233
Csp6I	GTAC	4 cut(s)	414, 637, 724, 1307
CviQI	GTAC	4 cut(s)	414, 637, 724, 1307
DdeI	CTNAG	3 cut(s)	875, 880, 1346
DpnI	GATC	4 cut(s)	131, 213, 644, 1095
DpnII	GATC	4 cut(s)	129, 211, 642, 1093
DraIII	CACNNNGTG	1 cut(s)	1010
EaeI	YGGCCR	1 cut(s)	174
Eam1104I	CTCTTC	2 cut(s)	912, 956
EarI	CTCTTC	2 cut(s)	912, 956
Eco130I	CCWWGG	3 cut(s)	108, 236, 1054
Eco57I	CTGAAG	5 cut(s)	108, 369, 405, 867, 937
EcoT14I	CCWWGG	3 cut(s)	108, 236, 1054
EcoT22I	ATGCAT	1 cut(s)	1281
ErhI	CCWWGG	3 cut(s)	108, 236, 1054
FaqI	GGGAC	1 cut(s)	1183
FauI	CCCGC	1 cut(s)	161
FbaI	TGATCA	1 cut(s)	642
FblI	GTMKAC	1 cut(s)	1399
Fnu4HI	GCNGC	4 cut(s)	459, 1038, 1240, 1267
FokI	GGATG	5 cut(s)	398, 551, 878, 1266, 1288
Fsp4HI	GCNGC	4 cut(s)	459, 1038, 1240, 1267
FspBI	CTAG	1 cut(s)	263
GlaI	GCGC	1 cut(s)	333
GluI	GCNGC	4 cut(s)	459, 1038, 1240, 1267
HaeIII	GGCC	3 cut(s)	15, 176, 234
HapII	CCGG	4 cut(s)	25, 216, 513, 1074
HhaI	GCGC	1 cut(s)	334
Hin6I	GCGC	1 cut(s)	332
HinP1I	GCGC	1 cut(s)	332
HinfI	GANTC	3 cut(s)	254, 549, 871
HpaII	CCGG	4 cut(s)	25, 216, 513, 1074
HphI	GGTGA	5 cut(s)	483, 798, 1154, 1202, 1304
Hpy166II	GTNNAC	2 cut(s)	1193, 1400
Hpy188I	TCNGA	7 cut(s)	154, 424, 760, 780, 855, 876, 956
Hpy188III	TCNNGA	4 cut(s)	313, 1129, 1415, 1456
Hpy8I	GTNNAC	2 cut(s)	1193, 1400
Hpy99I	CGWCG	2 cut(s)	191, 1440
HpyAV	CCTTC	4 cut(s)	293, 349, 695, 894
HpyCH4III	ACNGT	4 cut(s)	685, 1159, 1174, 1306
HpyCH4IV	ACGT	3 cut(s)	738, 960, 1119
HpyCH4V	TGCA	8 cut(s)	47, 323, 339, 461, 693, 1137, 1269, 1279
HpyF10VI	GCNNNNNNNGC	4 cut(s)	21, 154, 182, 329
HpyF3I	CTNAG	3 cut(s)	875, 880, 1346
HpySE526I	ACGT	3 cut(s)	738, 960, 1119
HspAI	GCGC	1 cut(s)	332
Ksp22I	TGATCA	1 cut(s)	642
Kzo9I	GATC	4 cut(s)	129, 211, 642, 1093
LmnI	GCTCC	3 cut(s)	28, 154, 344
Lsp1109I	GCAGC	4 cut(s)	445, 1024, 1251, 1253
LweI	GCATC	4 cut(s)	312, 1197, 1266, 1288
MaeI	CTAG	1 cut(s)	263
MaeII	ACGT	3 cut(s)	738, 960, 1119
MaeIII	GTNAC	2 cut(s)	1153, 1174
MalI	GATC	4 cut(s)	131, 213, 644, 1095
MboI	GATC	4 cut(s)	129, 211, 642, 1093
MboII	GAAGA	8 cut(s)	231, 383, 411, 746, 773, 790, 929, 943
MfeI	CAATTG	1 cut(s)	1375
MlsI	TGGCCA	1 cut(s)	176
MluCI	AATT	4 cut(s)	53, 389, 1032, 1375
MluNI	TGGCCA	1 cut(s)	176
MlyI	GAGTC	1 cut(s)	248
MmeI	TCCRAC	3 cut(s)	132, 686, 738
Mox20I	TGGCCA	1 cut(s)	176
Mph1103I	ATGCAT	1 cut(s)	1281
MroXI	GAANNNNTTC	1 cut(s)	738
MscI	TGGCCA	1 cut(s)	176
MseI	TTAA	4 cut(s)	657, 728, 834, 926
MslI	CAYNNNNRTG	2 cut(s)	241, 1274
Msp20I	TGGCCA	1 cut(s)	176
MspI	CCGG	4 cut(s)	25, 216, 513, 1074
MspR9I	CCNGG	3 cut(s)	26, 216, 1075
MunI	CAATTG	1 cut(s)	1375
MwoI	GCNNNNNNNGC	4 cut(s)	21, 154, 182, 329
NciI	CCSGG	3 cut(s)	26, 216, 1075
NcoI	CCATGG	2 cut(s)	108, 236
NdeII	GATC	4 cut(s)	129, 211, 642, 1093
NlaIV	GGNNCC	4 cut(s)	106, 156, 573, 1215
NsiI	ATGCAT	1 cut(s)	1281
NspI	RCATGY	3 cut(s)	327, 404, 1104
PaeI	GCATGC	2 cut(s)	327, 1104
PagI	TCATGA	1 cut(s)	1455
PaqCI	CACCTGC	1 cut(s)	820
PdmI	GAANNNNTTC	1 cut(s)	738
PfeI	GAWTC	2 cut(s)	549, 871
PflFI	GACNNNGTC	1 cut(s)	627
PfoI	TCCNGGA	2 cut(s)	214, 1073
PkrI	GCNGC	4 cut(s)	460, 1039, 1241, 1268
PleI	GAGTC	1 cut(s)	248
PpsI	GAGTC	1 cut(s)	248
Ppu21I	YACGTR	1 cut(s)	961
PspN4I	GGNNCC	4 cut(s)	106, 156, 573, 1215
PspPI	GGNCC	1 cut(s)	233
PsyI	GACNNNGTC	1 cut(s)	627
RsaI	GTAC	4 cut(s)	415, 638, 725, 1308
RsaNI	GTAC	4 cut(s)	414, 637, 724, 1307
RseI	CAYNNNNRTG	2 cut(s)	241, 1274
SaqAI	TTAA	4 cut(s)	657, 728, 834, 926
SatI	GCNGC	4 cut(s)	459, 1038, 1240, 1267
Sau3AI	GATC	4 cut(s)	129, 211, 642, 1093
Sau96I	GGNCC	1 cut(s)	233
ScaI	AGTACT	2 cut(s)	725, 1308
SchI	GAGTC	1 cut(s)	248
ScrFI	CCNGG	3 cut(s)	26, 216, 1075
SfaNI	GCATC	4 cut(s)	312, 1197, 1266, 1288
SfcI	CTRYAG	2 cut(s)	1105, 1287
SmiMI	CAYNNNNRTG	2 cut(s)	241, 1274
SmlI	CTYRAG	1 cut(s)	820
SmoI	CTYRAG	1 cut(s)	820
SphI	GCATGC	2 cut(s)	327, 1104
Sse9I	AATT	4 cut(s)	53, 389, 1032, 1375
SsiI	CCGC	4 cut(s)	5, 22, 168, 1188
SspMI	CTAG	1 cut(s)	263
StyD4I	CCNGG	3 cut(s)	24, 214, 1073
StyI	CCWWGG	3 cut(s)	108, 236, 1054
TaaI	ACNGT	4 cut(s)	685, 1159, 1174, 1306
TaiI	ACGT	3 cut(s)	741, 963, 1122
TaqI	TCGA	3 cut(s)	51, 547, 1096
TasI	AATT	4 cut(s)	53, 389, 1032, 1375
TatI	WGTACW	3 cut(s)	413, 723, 1306
TfiI	GAWTC	2 cut(s)	549, 871
Tru1I	TTAA	4 cut(s)	657, 728, 834, 926
Tru9I	TTAA	4 cut(s)	657, 728, 834, 926
TscAI	CASTG	1 cut(s)	688
TseI	GCWGC	4 cut(s)	458, 1037, 1239, 1266
TspDTI	ATGAA	6 cut(s)	399, 402, 627, 714, 820, 1077
TspGWI	ACGGA	3 cut(s)	84, 357, 959
TspRI	CASTG	1 cut(s)	688
Tth111I	GACNNNGTC	1 cut(s)	627
XapI	RAATTY	2 cut(s)	53, 389
XceI	RCATGY	3 cut(s)	327, 404, 1104
XcmI	CCANNNNNNNNNTGG	1 cut(s)	476
XmiI	GTMKAC	1 cut(s)	1399
XmnI	GAANNNNTTC	1 cut(s)	738
XspI	CTAG	1 cut(s)	263
ZrmI	AGTACT	2 cut(s)	725, 1308
Zsp2I	ATGCAT	1 cut(s)	1281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.