Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0013522

Physical Location & Seq

Genomic Coordinates Reverse (-)

32724906 .. 32731239

Gene Structure 6334 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1199600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGATGTTCCGTAGGTTGAAGGGTGTGACATCTCTTGGCATTTTAAAGTGGAAAGGCGATTCATCTTTGACATCAGGGTTGCTTAGGGATGTGGCTCCTGAGGTTGAATTATCTGACTATGGTAGGGCACCGAGTCCTGGTAGTGAGAGCCCTTCAGGGCTTCTTAATGGTGAGAGTATAAATGTTGAACCAATTGCTGATTTGGACCTGTTCTTTGAAAGGCTCTACAGCTACTACTGTGAGAAAGGACTTTGGTGTATTATTATAAAGTGGATAGTTGAACTTTTGAGTTTGGGCTTCACTATATTGTTCTCAGGATTCTTCTTATTAAAAGTTGATTGGAATGGTCTTCGTAATGCAAAATGTGGGATGGATGCATTCGAATCTGGAATTAAGCCCTGTGACCTTGCTAAGGAAGCCCTTCATCAGCACCCATTAACCCCTCTAACTCTTTCCAAAGCCATCATTGTTGGATATTTGGGCATATTTTCCATTTATTTGGTCTTCTGTTTTTTGAGGTTTTTCTCTCAGTTAAGGGATACTTTGGCAGTCCGTCACTTCTATCACAACAGTCTCCATGTCACAGACAATGAAATTCAGACCATGCCATGGGCATCAATTCTTGAAAAGGTTGTTCAGCTACAAAGATCGCAGCAGCTCTGTGTGGTCAAAGATCTTTCTGCTCATGATGTGGTTATGCGATTGATGCGGAAGGAGAACTACTTGATTGGAATGCTTAACAAGGGGGTGCTTGCTTTCCCAATTTCACAGTGGGTTCCAGGTGCTGGTCCAATTGTAAAGTCTGGCTCGGATGGAAAGCAAGAACGATTAATACTGACAAAAACCCTCGAGTGGACCTTAAATTGGTGCATTCTGCAGAGCATGTTTGATCGAAATTTCTATGTTACAAGGGACTTTAAATCTAATCCAAGAACATTAAAGAAAAGGCTTATGGTAGTTGGATTCGTAATGCTTCTCCTTTCCCCATTTCTTGTAATATTCATGCTGGTATATCTCTTCCTGAGGCATGCAGAACAGTTTTATAACCATCCAAGCACAGCATCATCTCGAAGGTGGTCAAATTTGTCAAGGTGGATGTTTAGGGAATTTAATGAGGTGGACCATTTGTTTAAACACCGAGTCACGAGCAGTGTAGTGCATGCTTCTGATTATCTGAAGCAATTTCCTTGCCCTATTATATCCATAATAGCGAAATTCATCTCTTTTGTTTCTGGTGGCTTTGCTGCTATCCTAATCATCATCGCATTTCTGGAGGAATCTCTGCTAGAGGGCCATATATTTGGACGCAACTTGTTCTGGTATGCTGCTGTTTTTGGAACTATAACAGCTATTAGCAGGGCTGCCATTTCCGATGAACTGCTGGTCCTTGATCCAGAGGGGGCAATGTCTATGGTGGTACAATATACACATTATATGCCAAAGACATGGCGTGGCAAAGAGAATACTGAAAGGGTTCGGGTAGAGTTTGAAACCCTGTTTCAGTATACTGGAATGATGTTACTTGAGGAGATGGCCTCAATCTTCCTTACTCCATATTTACTTATATTTGTCGTCCCGAAGAGGGTGGATGACATTTTACAGTTTATTGCAGACTTCACAGTAAATGTTGAGGGTGTTGGTCATGTGTGTAGTTTTAGCACCTTCGACTTTGAAAGACATGGTAATAGCAATTACGGGTCACCTGACAATGTACCTCGCTCGCAGAGGAGTTCTCAGGGGAAAATGGAGAAATCGTTTTTGAGCTTCAAGAGTAACTATCCATCGTGGGACCCAAATACTCAAGGTGATCAGTTTCTTAAAAAGCTGAGAACTTTCAGGGAGCAAAAGTTGCAGGGACAAGGAACTCGACATGCATATTCTCCTCCGAGAGGATTTGGCGACCGCAACAATCTTTTATCATGGGAAAGGCCATATCATAGCCCTGGAACTGCCTATCACTTGGGGTCTCTGTGGTTAATTGATGCAGATCAAAAGAATCACCACCCATATCTCCTTGATTGGTATTACACCTCTCGGCCACACCGCATGGCAAGTTACGCTGGTGAAATTCCAGAAGAACCATTTGAAGCTACTGAGCAACATTCTACTGATTGGACCCCAACCAACTTTCCAGATAATCAACTGAGATTTGAAGATCTCTGGGCTCATCGTTATGATGACCGTTCACAGTCTAATCTGGGGGCTTCTACATCAGCGCCTTTCCACCGAGAGAGTGTACTTCAGCATCATGACGCTGGCACTTCCGCAAATCCAATGCGGAGTCATTGGTGGGCTAGAACCGGCCAACACGGAACACAGCCCCAGTCAAGTTTTCTCGAGCCTCCAGATTTCACTCAACACGGGAAACAACCCCAGTCAAGCTTTCTCGATCCTCCAAACTTCATGCGCCAGTCTTCAGATAATTTTTACGGAAACTTCTCAGAAAGAAGCTTCGAGGAACACGAACAAGAAGAAGAACCGGAACAAGAACAAGAACAACGGTTGGACTGGAGGAACTACCAGAGCTTGTCCCGAACGACGTACGTGGATGACCTGGACTTGGAGGCAGGAGAGTTTAATCTTCATTTCGGTGACGTGTACAGTAGTAGACCTCCTGAAACTCCGAAAATAAAGCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005776 GO:0006914 GO:0006950 GO:0006952 GO:0006996 GO:0007005 GO:0007033 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016043 GO:0016236 GO:0022411 GO:0022607 GO:0033036 GO:0034497 GO:0034613 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044237 GO:0044248 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0044805 GO:0050832 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0061726 GO:0061919 GO:0070727 GO:0070925 GO:0071840 GO:0098542 GO:1903008 GO:1905037

KEGG Pathways

Metabolic & Signaling

ko04136 ko04137 ko04138 ko04140 map04136 map04137 map04138 map04140

Pfam Domains

Protein Families

APG9

Protein Analysis

880

Amino Acids

101.39

Weight (kDa)

6.29

Isoelectric Point (pI)

44.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG9	PF04109	62 - 556	3.2e-163	Autophagy protein ATG9

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013522)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31260
fragaria_vesca	FvH4_2g21710
malus_domestica	MD05G1199600.v1.1 MD10G1185900.v1.1
prunus_persica	Prupe.8G225400_v2.0.a1 Prupe.8G225400_v2.0.a1
pyrus_communis	pycom05g18600 pycom10g16080
rosa_chinensis	RchiOBHm_Chr6g0288391
rosa_laevigata	RLG00000012408
rosa_multiflora	Rmu_sc0001038.1_g000011
rosa_roxburghii	Rroxscaffold_7G00180100
rosa_rugosa	Rorug06G0196200
rosa_samantha	Rh6AG307100 Rh6BG313100 Rh6CG320900 Rh6DG306300
rosa_wichuraiana	Rw6G026570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	266, 1044
AccB1I	GGYRCC	1 cut(s)	127
AccB7I	CCANNNNNTGG	2 cut(s)	609, 785
AccI	GTMKAC	2 cut(s)	1505, 2608
AciI	CCGC	5 cut(s)	709, 1903, 2044, 2265, 2278
AclWI	GGATC	2 cut(s)	1385, 2384
AcoI	YGGCCR	2 cut(s)	2036, 2302
AcsI	RAATTY	6 cut(s)	594, 895, 1081, 1106, 1214, 2067
AcuI	CTGAAG	4 cut(s)	138, 1196, 2225, 2400
AfaI	GTAC	5 cut(s)	1419, 1713, 2238, 2543, 2600
AfiI	CCNNNNNNNGG	9 cut(s)	137, 412, 609, 785, 852, 864, 1582, 1889, 2560
AflIII	ACRYGT	1 cut(s)	2595
AjiI	CACGTC	1 cut(s)	2596
AjnI	CCWGG	4 cut(s)	136, 778, 1942, 2553
AloI	GAACNNNNNNTCC	2 cut(s)	1368, 1400
Alw26I	GTCTC	2 cut(s)	578, 1971
AlwI	GGATC	2 cut(s)	1385, 2384
AlwNI	CAGNNNCTG	1 cut(s)	785
Ama87I	CYCGRG	2 cut(s)	848, 2336
AoxI	GGCC	5 cut(s)	1291, 1533, 1928, 2036, 2302
ApeKI	GCWGC	5 cut(s)	652, 655, 1244, 1325, 1361
ApoI	RAATTY	6 cut(s)	594, 895, 1081, 1106, 1214, 2067
ArsI	GACNNNNNNTTYG	2 cut(s)	197, 229
AseI	ATTAAT	1 cut(s)	830
Asp700I	GAANNNNTTC	2 cut(s)	420, 1473
AspLEI	GCGC	2 cut(s)	2218, 2409
AspS9I	GGNCC	8 cut(s)	205, 788, 855, 1120, 1291, 1384, 1789, 2115
AsuHPI	GGTGA	6 cut(s)	182, 1692, 1817, 1991, 2075, 2603
AsuII	TTCGAA	1 cut(s)	381
AvaI	CYCGRG	2 cut(s)	848, 2336
AvaII	GGWCC	7 cut(s)	205, 788, 855, 1120, 1384, 1789, 2115
AxyI	CCTNAGG	2 cut(s)	99, 1022
BaeGI	GKGCMC	1 cut(s)	130
BanI	GGYRCC	1 cut(s)	127
BanII	GRGCYC	2 cut(s)	152, 2167
BarI	GAAGNNNNNNTAC	2 cut(s)	704, 736
BauI	CACGAG	1 cut(s)	1144
BbsI	GAAGAC	3 cut(s)	341, 496, 2406
BbvI	GCAGC	5 cut(s)	664, 667, 1231, 1312, 1348
BccI	CCATC	6 cut(s)	364, 470, 806, 1056, 1525, 1789
BciT130I	CCWGG	4 cut(s)	138, 780, 1944, 2555
BciVI	GTATCC	1 cut(s)	532
BclI	TGATCA	1 cut(s)	1807
BcoDI	GTCTC	2 cut(s)	578, 1971
BfaI	CTAG	2 cut(s)	1286, 2295
BfmI	CTRYAG	2 cut(s)	226, 875
BfoI	RGCGCY	1 cut(s)	2219
BfuI	GTATCC	1 cut(s)	532
BglII	AGATCT	2 cut(s)	673, 2155
BisI	GCNGC	5 cut(s)	653, 656, 1245, 1326, 1362
BlsI	GCNGC	5 cut(s)	654, 657, 1246, 1327, 1363
Bme1390I	CCNGG	4 cut(s)	138, 780, 1944, 2555
Bme18I	GGWCC	7 cut(s)	205, 788, 855, 1120, 1384, 1789, 2115
BmeT110I	CYCGRG	2 cut(s)	848, 2336
BmgBI	CACGTC	1 cut(s)	2596
BmgT120I	GGNCC	8 cut(s)	205, 788, 855, 1120, 1291, 1384, 1789, 2115
BmiI	GGNNCC	6 cut(s)	96, 129, 777, 1790, 1791, 2117
BmrFI	CCNGG	4 cut(s)	138, 780, 1944, 2555
BmrI	ACTGGG	2 cut(s)	2317, 2368
BmsI	GCATC	6 cut(s)	364, 623, 696, 1070, 1972, 2254
BmuI	ACTGGG	2 cut(s)	2317, 2368
BpiI	GAAGAC	3 cut(s)	341, 496, 2406
BplI	GAGNNNNNCTC	4 cut(s)	1520, 1552, 1717, 1749
BpmI	CTGGAG	3 cut(s)	1292, 2328, 2530
Bpu10I	CCTNAGC	2 cut(s)	83, 411
Bpu14I	TTCGAA	1 cut(s)	381
BpuEI	CTTGAG	2 cut(s)	1544, 1785
BsaAI	YACGTR	1 cut(s)	2545
BsaBI	GATNNNNATC	1 cut(s)	1986
BsaI	GGTCTC	1 cut(s)	1971
BsaJI	CCNNGG	2 cut(s)	608, 1942
BsaWI	WCCGGW	1 cut(s)	2479
BsaXI	ACNNNNNCTCC	4 cut(s)	1739, 1769, 1832, 1862
Bsc4I	CCNNNNNNNGG	9 cut(s)	137, 412, 609, 785, 852, 864, 1582, 1889, 2560
Bse118I	RCCGGY	1 cut(s)	2300
Bse1I	ACTGG	5 cut(s)	1513, 2323, 2374, 2410, 2513
Bse21I	CCTNAGG	2 cut(s)	99, 1022
Bse3DI	GCAATG	1 cut(s)	1410
Bse8I	GATNNNNATC	1 cut(s)	1986
BseBI	CCWGG	4 cut(s)	138, 780, 1944, 2555
BseDI	CCNNGG	2 cut(s)	608, 1942
BseGI	GGATG	8 cut(s)	94, 375, 379, 817, 1048, 1101, 1594, 2554
BseJI	GATNNNNATC	1 cut(s)	1986
BseLI	CCNNNNNNNGG	9 cut(s)	137, 412, 609, 785, 852, 864, 1582, 1889, 2560
BseMI	GCAATG	1 cut(s)	1410
BseMII	CTCAG	9 cut(s)	90, 327, 542, 1013, 1748, 1817, 2085, 2135, 2454
BseNI	ACTGG	5 cut(s)	1513, 2323, 2374, 2410, 2513
BseRI	GAGGAG	3 cut(s)	1541, 1741, 1872
BseSI	GKGCMC	1 cut(s)	130
BseXI	GCAGC	5 cut(s)	664, 667, 1231, 1312, 1348
Bsh1285I	CGRYCG	1 cut(s)	1903
BshFI	GGCC	5 cut(s)	1293, 1535, 1930, 2038, 2304
BshNI	GGYRCC	1 cut(s)	127
BsiEI	CGRYCG	1 cut(s)	1903
BsiHKCI	CYCGRG	2 cut(s)	848, 2336
BsiSI	CCGG	2 cut(s)	2301, 2480
BsiWI	CGTACG	1 cut(s)	2541
BslFI	GGGAC	5 cut(s)	926, 1559, 1802, 1869, 2515
BslI	CCNNNNNNNGG	9 cut(s)	137, 412, 609, 785, 852, 864, 1582, 1889, 2560
BsmAI	GTCTC	2 cut(s)	578, 1971
BsmFI	GGGAC	5 cut(s)	926, 1559, 1802, 1869, 2515
BsmI	GAATGC	3 cut(s)	377, 738, 870
BsnI	GGCC	5 cut(s)	1293, 1535, 1930, 2038, 2304
Bso31I	GGTCTC	1 cut(s)	1971
BsoBI	CYCGRG	2 cut(s)	848, 2336
Bsp119I	TTCGAA	1 cut(s)	381
Bsp1286I	GDGCHC	3 cut(s)	130, 152, 2167
Bsp1407I	TGTACA	1 cut(s)	2598
Bsp143I	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
Bsp19I	CCATGG	1 cut(s)	608
BspACI	CCGC	5 cut(s)	709, 1903, 2044, 2265, 2278
BspANI	GGCC	5 cut(s)	1293, 1535, 1930, 2038, 2304
BspCNI	CTCAG	9 cut(s)	91, 326, 541, 1014, 1747, 1818, 2086, 2136, 2453
BspHI	TCATGA	2 cut(s)	685, 2248
BspLI	GGNNCC	6 cut(s)	96, 129, 777, 1790, 1791, 2117
BspMAI	CTGCAG	1 cut(s)	879
BspPI	GGATC	2 cut(s)	1385, 2384
BspT104I	TTCGAA	1 cut(s)	381
BspT107I	GGYRCC	1 cut(s)	127
BspTNI	GGTCTC	1 cut(s)	1971
BsrDI	GCAATG	1 cut(s)	1410
BsrFI	RCCGGY	1 cut(s)	2300
BsrGI	TGTACA	1 cut(s)	2598
BsrI	ACTGG	5 cut(s)	1513, 2323, 2374, 2410, 2513
BssAI	RCCGGY	1 cut(s)	2300
BssECI	CCNNGG	2 cut(s)	608, 1942
BssMI	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
BssNAI	GTATAC	1 cut(s)	1506
BssSI	CACGAG	1 cut(s)	1144
BssT1I	CCWWGG	1 cut(s)	608
Bst1107I	GTATAC	1 cut(s)	1506
Bst2BI	CACGAG	1 cut(s)	1144
Bst2UI	CCWGG	4 cut(s)	138, 780, 1944, 2555
Bst6I	CTCTTC	2 cut(s)	1022, 1574
BstAPI	GCANNNNNTGC	1 cut(s)	1849
BstAUI	TGTACA	1 cut(s)	2598
BstBAI	YACGTR	1 cut(s)	2545
BstBI	TTCGAA	1 cut(s)	381
BstC8I	GCNNGC	5 cut(s)	753, 1029, 1161, 1721, 2257
BstDSI	CCRYGG	1 cut(s)	608
BstEII	GGTNACC	1 cut(s)	1698
BstENI	CCTNNNNNAGG	2 cut(s)	410, 1887
BstF5I	GGATG	8 cut(s)	94, 375, 379, 817, 1048, 1101, 1594, 2554
BstH2I	RGCGCY	1 cut(s)	2219
BstHHI	GCGC	2 cut(s)	2218, 2409
BstKTI	GATC	8 cut(s)	650, 676, 892, 1393, 1810, 1990, 2158, 2392
BstMAI	GTCTC	2 cut(s)	578, 1971
BstMBI	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
BstMCI	CGRYCG	1 cut(s)	1903
BstMWI	GCNNNNNNNGC	4 cut(s)	416, 706, 1849, 2057
BstNI	CCWGG	4 cut(s)	138, 780, 1944, 2555
BstNSI	RCATGY	4 cut(s)	886, 1031, 1163, 1874
BstPI	GGTNACC	1 cut(s)	1698
BstSCI	CCNGG	4 cut(s)	136, 778, 1942, 2553
BstSFI	CTRYAG	2 cut(s)	226, 875
BstSLI	GKGCMC	1 cut(s)	130
BstV1I	GCAGC	5 cut(s)	664, 667, 1231, 1312, 1348
BstV2I	GAAGAC	3 cut(s)	341, 496, 2406
BstX2I	RGATCY	2 cut(s)	673, 2155
BstXI	CCANNNNNNTGG	3 cut(s)	499, 1301, 1446
BstYI	RGATCY	2 cut(s)	673, 2155
BstZ17I	GTATAC	1 cut(s)	1506
Bsu36I	CCTNAGG	2 cut(s)	99, 1022
BsuI	GTATCC	1 cut(s)	532
BsuRI	GGCC	5 cut(s)	1293, 1535, 1930, 2038, 2304
BtgI	CCRYGG	1 cut(s)	608
BtgZI	GCGATG	1 cut(s)	1246
BtrI	CACGTC	1 cut(s)	2596
BtsCI	GGATG	8 cut(s)	94, 375, 379, 817, 1048, 1101, 1594, 2554
BtsI	GCAGTG	1 cut(s)	1156
BtsIMutI	CAGTG	2 cut(s)	776, 1156
Cac8I	GCNNGC	5 cut(s)	753, 1029, 1161, 1721, 2257
CaiI	CAGNNNCTG	1 cut(s)	785
CciI	TCATGA	2 cut(s)	685, 2248
CfoI	GCGC	2 cut(s)	2218, 2409
Cfr10I	RCCGGY	1 cut(s)	2300
Cfr13I	GGNCC	8 cut(s)	205, 788, 855, 1120, 1291, 1384, 1789, 2115
CseI	GACGC	2 cut(s)	1314, 2261
Csp6I	GTAC	5 cut(s)	1418, 1712, 2237, 2542, 2599
CviQI	GTAC	5 cut(s)	1418, 1712, 2237, 2542, 2599
DpnI	GATC	8 cut(s)	649, 675, 891, 1392, 1809, 1989, 2157, 2391
DpnII	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
DraI	TTTAAA	3 cut(s)	45, 919, 1132
EaeI	YGGCCR	2 cut(s)	2036, 2302
Eam1104I	CTCTTC	2 cut(s)	1022, 1574
EarI	CTCTTC	2 cut(s)	1022, 1574
Eco130I	CCWWGG	1 cut(s)	608
Eco24I	GRGCYC	2 cut(s)	152, 2167
Eco31I	GGTCTC	1 cut(s)	1971
Eco47I	GGWCC	7 cut(s)	205, 788, 855, 1120, 1384, 1789, 2115
Eco57I	CTGAAG	4 cut(s)	138, 1196, 2225, 2400
Eco81I	CCTNAGG	2 cut(s)	99, 1022
Eco88I	CYCGRG	2 cut(s)	848, 2336
Eco91I	GGTNACC	1 cut(s)	1698
EcoNI	CCTNNNNNAGG	2 cut(s)	410, 1887
EcoO109I	RGGNCCY	1 cut(s)	1789
EcoO65I	GGTNACC	1 cut(s)	1698
EcoRII	CCWGG	4 cut(s)	136, 778, 1942, 2553
EcoT14I	CCWWGG	1 cut(s)	608
EcoT22I	ATGCAT	2 cut(s)	379, 1876
EcoT38I	GRGCYC	2 cut(s)	152, 2167
ErhI	CCWWGG	1 cut(s)	608
FalI	AAGNNNNNCTT	2 cut(s)	526, 558
FaqI	GGGAC	5 cut(s)	926, 1559, 1802, 1869, 2515
FbaI	TGATCA	1 cut(s)	1807
FblI	GTMKAC	2 cut(s)	1505, 2608
Fnu4HI	GCNGC	5 cut(s)	653, 656, 1245, 1326, 1362
FokI	GGATG	8 cut(s)	101, 382, 386, 824, 1035, 1108, 1601, 2561
FriOI	GRGCYC	2 cut(s)	152, 2167
Fsp4HI	GCNGC	5 cut(s)	653, 656, 1245, 1326, 1362
FspBI	CTAG	2 cut(s)	1286, 2295
GlaI	GCGC	2 cut(s)	2217, 2408
GluI	GCNGC	5 cut(s)	653, 656, 1245, 1326, 1362
GsuI	CTGGAG	3 cut(s)	1292, 2328, 2530
HaeII	RGCGCY	1 cut(s)	2219
HaeIII	GGCC	5 cut(s)	1293, 1535, 1930, 2038, 2304
HapII	CCGG	2 cut(s)	2301, 2480
HgaI	GACGC	2 cut(s)	1314, 2261
HhaI	GCGC	2 cut(s)	2218, 2409
Hin6I	GCGC	2 cut(s)	2216, 2407
HinP1I	GCGC	2 cut(s)	2216, 2407
HindIII	AAGCTT	2 cut(s)	2380, 2449
HinfI	GANTC	9 cut(s)	59, 133, 318, 383, 963, 1140, 1277, 1996, 2281
HpaII	CCGG	2 cut(s)	2301, 2480
HphI	GGTGA	6 cut(s)	182, 1692, 1817, 1991, 2075, 2603
Hpy166II	GTNNAC	7 cut(s)	855, 1120, 1506, 2186, 2237, 2599, 2609
Hpy8I	GTNNAC	7 cut(s)	855, 1120, 1506, 2186, 2237, 2599, 2609
Hpy99I	CGWCG	1 cut(s)	2542
HpyAV	CCTTC	6 cut(s)	13, 162, 431, 706, 1065, 1672
HpyCH4IV	ACGT	3 cut(s)	2540, 2544, 2595
HpyF10VI	GCNNNNNNNGC	4 cut(s)	416, 706, 1849, 2057
HpySE526I	ACGT	3 cut(s)	2540, 2544, 2595
HspAI	GCGC	2 cut(s)	2216, 2407
KflI	GGGWCCC	1 cut(s)	1789
Ksp22I	TGATCA	1 cut(s)	1807
Kzo9I	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
LmnI	GCTCC	2 cut(s)	100, 1840
Lsp1109I	GCAGC	5 cut(s)	664, 667, 1231, 1312, 1348
LweI	GCATC	6 cut(s)	364, 623, 696, 1070, 1972, 2254
MaeI	CTAG	2 cut(s)	1286, 2295
MaeII	ACGT	3 cut(s)	2540, 2544, 2595
MalI	GATC	8 cut(s)	649, 675, 891, 1392, 1809, 1989, 2157, 2391
MboI	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
MfeI	CAATTG	2 cut(s)	192, 792
MflI	RGATCY	2 cut(s)	673, 2155
MhlI	GDGCHC	3 cut(s)	130, 152, 2167
MlyI	GAGTC	3 cut(s)	142, 1149, 2290
MmeI	TCCRAC	3 cut(s)	451, 940, 2484
Mph1103I	ATGCAT	2 cut(s)	379, 1876
MroXI	GAANNNNTTC	2 cut(s)	420, 1473
MslI	CAYNNNNRTG	2 cut(s)	2172, 2589
MspI	CCGG	2 cut(s)	2301, 2480
MspR9I	CCNGG	4 cut(s)	138, 780, 1944, 2555
MssI	GTTTAAAC	1 cut(s)	1132
MunI	CAATTG	2 cut(s)	192, 792
Mva1269I	GAATGC	3 cut(s)	377, 738, 870
MvaI	CCWGG	4 cut(s)	138, 780, 1944, 2555
MwoI	GCNNNNNNNGC	4 cut(s)	416, 706, 1849, 2057
NcoI	CCATGG	1 cut(s)	608
NdeII	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
NlaIV	GGNNCC	6 cut(s)	96, 129, 777, 1790, 1791, 2117
NmeAIII	GCCGAG	1 cut(s)	2014
NmuCI	GTSAC	7 cut(s)	25, 401, 554, 580, 1141, 1698, 2591
NsiI	ATGCAT	2 cut(s)	379, 1876
NspI	RCATGY	4 cut(s)	886, 1031, 1163, 1874
NspV	TTCGAA	1 cut(s)	381
PaeI	GCATGC	2 cut(s)	1031, 1163
PaeR7I	CTCGAG	2 cut(s)	848, 2336
PagI	TCATGA	2 cut(s)	685, 2248
PcsI	WCGNNNNNNNCGW	1 cut(s)	2460
PctI	GAATGC	3 cut(s)	377, 738, 870
PdmI	GAANNNNTTC	2 cut(s)	420, 1473
PfeI	GAWTC	6 cut(s)	59, 318, 383, 963, 1277, 1996
Pfl23II	CGTACG	1 cut(s)	2541
PflMI	CCANNNNNTGG	2 cut(s)	609, 785
PkrI	GCNGC	5 cut(s)	654, 657, 1246, 1327, 1363
PleI	GAGTC	3 cut(s)	141, 1148, 2289
PmeI	GTTTAAAC	1 cut(s)	1132
PpsI	GAGTC	3 cut(s)	141, 1148, 2289
Ppu21I	YACGTR	1 cut(s)	2545
PpuMI	RGGWCCY	1 cut(s)	1789
PshBI	ATTAAT	1 cut(s)	830
PsiI	TTATAA	2 cut(s)	266, 1044
Psp5II	RGGWCCY	1 cut(s)	1789
Psp6I	CCWGG	4 cut(s)	136, 778, 1942, 2553
PspEI	GGTNACC	1 cut(s)	1698
PspGI	CCWGG	4 cut(s)	136, 778, 1942, 2553
PspLI	CGTACG	1 cut(s)	2541
PspN4I	GGNNCC	6 cut(s)	96, 129, 777, 1790, 1791, 2117
PspPI	GGNCC	8 cut(s)	205, 788, 855, 1120, 1291, 1384, 1789, 2115
PspPPI	RGGWCCY	1 cut(s)	1789
PspXI	VCTCGAGB	1 cut(s)	848
PsrI	GAACNNNNNNTAC	2 cut(s)	816, 848
PstI	CTGCAG	1 cut(s)	879
PstNI	CAGNNNCTG	1 cut(s)	785
PsuI	RGATCY	2 cut(s)	673, 2155
RsaI	GTAC	5 cut(s)	1419, 1713, 2238, 2543, 2600
RsaNI	GTAC	5 cut(s)	1418, 1712, 2237, 2542, 2599
RseI	CAYNNNNRTG	2 cut(s)	2172, 2589
SatI	GCNGC	5 cut(s)	653, 656, 1245, 1326, 1362
Sau3AI	GATC	8 cut(s)	647, 673, 889, 1390, 1807, 1987, 2155, 2389
Sau96I	GGNCC	8 cut(s)	205, 788, 855, 1120, 1291, 1384, 1789, 2115
SchI	GAGTC	3 cut(s)	142, 1149, 2290
ScrFI	CCNGG	4 cut(s)	138, 780, 1944, 2555
SduI	GDGCHC	3 cut(s)	130, 152, 2167
SfaNI	GCATC	6 cut(s)	364, 623, 696, 1070, 1972, 2254
SfcI	CTRYAG	2 cut(s)	226, 875
Sfr274I	CTCGAG	2 cut(s)	848, 2336
SfuI	TTCGAA	1 cut(s)	381
SinI	GGWCC	7 cut(s)	205, 788, 855, 1120, 1384, 1789, 2115
SlaI	CTCGAG	2 cut(s)	848, 2336
SmiMI	CAYNNNNRTG	2 cut(s)	2172, 2589
SmlI	CTYRAG	4 cut(s)	848, 1523, 1800, 2336
SmoI	CTYRAG	4 cut(s)	848, 1523, 1800, 2336
SphI	GCATGC	2 cut(s)	1031, 1163
SsiI	CCGC	5 cut(s)	709, 1903, 2044, 2265, 2278
SspI	AATATT	1 cut(s)	999
SspMI	CTAG	2 cut(s)	1286, 2295
StyD4I	CCNGG	4 cut(s)	136, 778, 1942, 2553
StyI	CCWWGG	1 cut(s)	608
TaiI	ACGT	3 cut(s)	2543, 2547, 2598
TaqI	TCGA	9 cut(s)	381, 849, 892, 1069, 1665, 1867, 2337, 2388, 2454
TatI	WGTACW	2 cut(s)	2236, 2598
TfiI	GAWTC	6 cut(s)	59, 318, 383, 963, 1277, 1996
TscAI	CASTG	2 cut(s)	776, 1156
TseFI	GTSAC	7 cut(s)	25, 401, 554, 580, 1141, 1698, 2591
TseI	GCWGC	5 cut(s)	652, 655, 1244, 1325, 1361
Tsp45I	GTSAC	7 cut(s)	25, 401, 554, 580, 1141, 1698, 2591
TspDTI	ATGAA	8 cut(s)	51, 413, 606, 991, 1207, 1389, 2392, 2573
TspGWI	ACGGA	3 cut(s)	542, 2325, 2445
TspRI	CASTG	2 cut(s)	776, 1156
Van91I	CCANNNNNTGG	2 cut(s)	609, 785
VpaK11BI	GGWCC	7 cut(s)	205, 788, 855, 1120, 1384, 1789, 2115
VspI	ATTAAT	1 cut(s)	830
XagI	CCTNNNNNAGG	2 cut(s)	410, 1887
XapI	RAATTY	6 cut(s)	594, 895, 1081, 1106, 1214, 2067
XceI	RCATGY	4 cut(s)	886, 1031, 1163, 1874
XcmI	CCANNNNNNNNNTGG	1 cut(s)	768
XhoI	CTCGAG	2 cut(s)	848, 2336
XmiI	GTMKAC	2 cut(s)	1505, 2608
XmnI	GAANNNNTTC	2 cut(s)	420, 1473
XspI	CTAG	2 cut(s)	1286, 2295
Zsp2I	ATGCAT	2 cut(s)	379, 1876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.