MD05G1201700.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003080
Physical Location & Seq
Reverse (-)
32881150 .. 32891386
10237 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1201700.v1.1.491

Sequence Viewer

Length: 3486 bp
ATGGCGAAAATGTGGGAAGTGCGAAGACTGCCACTTCCACAAACCAAACGACACGAACAACGCAACCCCAAACCAACGAAGGACAAAGGGAAGAAAAGCAAAGAAAGGGCCAAGCCAGTGACCATTGTTGTTGGTTCTCATATTTGGGTTGAAAACCCAGTTTGGATTGACGGAGAAGTACTAAACATTAAGGGAGATGACGCTGAGATTCAAACTAGTGGTGGGAGTAAGGTTGTTGCAAAGCTGTCAAAAATATATCCAAAGGATACAGAAACTCCTCCTGGTGGAGTTGATGACATGACTAAGCTATCATACTTGCATGAACCCGGAGTGCTTCACAATTTGGCAACTAGATATGAGATAAATGAAATTTATACTTACACTGGAAACATTCTCATCGCCATCAATCCTTTCCAAAGTCTCTCACATCTGTATGATGCTGATATGATGGAACGGTATAATGGAGTACCATATGGAGAACTGAGGCCTCATGTTTTTGCAATAGCCGATGCTGCATACAGGGAAATGATTAAGGAGGGAAAAGGAAACTCGATTCTGGTTAGTGGGGAAAGCGGAGCTGGTAAGACCGAAACAACTAAAATGCTTATGCGCTACCTTGCCTATTTGGGTGGTAATGCTGCAGCTGAAGGGCGGACTGTTGAACAAAAAGTTTTAGAATCAAATCCAGTTCTTGAAGCATTTGGTAACGCTAAGACTGTTAGGAATAACAATTCCAGCCGCTTTGGGAAATTTGTCGAGATTCAATTTGATAAATGTGGTAGAATATCAGGGGCAGCCATTAGAACATATCTCTTAGAGAGATCTCGTGTTTGCCAGATTTCGGATTCAGAGCGTAACTATCACTGTTTTTACCTCCTCTGTGCTGCACCGCCTCAGGAAATAGAGAAATATAAGTTGGGAGAACCTAAGTTGTATCACTATCTTAATCAATCAAATTGCTATGAACTTGATGGCACAAGTGATCAGCACAATTATCTCTCCACTCGGAGAGCCATGGATGTTGTTGGAATAAATGCAGTAGAACAGGAGGCTATTTTCAGAGTTGTAGCTGCAATTCTTCATCTTGGGAATATTGATTTTGCCAATGGGGGAGATAATGACTCATCTGTTCTGAAAAATGACGAATCCCTTTTTCATCTTCAAATGACAGCAGAACTTCTCATGTGTAATCCTCGGGCTCTGGAAGATGCACTGTGTAAGCGTGTAATGGTTACTCCAGAAGAAATTATTAAAAAAAGTCTTGATCCCCATGGCGCAACAGTTAGCAGGGATGGATTGGCCAAGACTATATATTCTCGTTTGTTTGACTGGTTGGTGGATAAGATCAATTTCTCAATTGGACAGGACCCTAACTCCAAATGTCTGATTGGGGTTCTTGATATATATGGTTTTGAAAGCTTCAAAACTAACAGTTTTGAGCAGTTCTGTATTAATTACACTAATGAAAAGCTGCAACAACATTTCAACAAGCATGTATTCAAGACGCAACAAGAGGAATACACTAAAGAGGAAATCGATTGGAGCTACATAGAATTTGTTGATAATAAAGATGTCCTTGATCTCATTGAACAGAAGAAAGGGGGGATTATTGCTCTGCTTGATGAAGCTTGTATGTTTCCAAAATCAACTCATGAAACATTTTCAACAAAGCTGTATCAGACATTCAAAGATCACAAACGCTTCATCAAGCCAAAACTGACCCGCTCAGATTTCACCATTGTTCATTATGCAGGAGAAGTGGCCTTGGTTCCTGTACAATATCAGTCTGATCAATTTCTTGACAAAAATAAAGACTATATTGTTCCTGAACATCGAGATTTGTTGAGTGCATCGACATGTTCTTTTGTAGCAGGCCTTTTCCCTCCACTTACTGAGGAGGTGGCCAAATCTTCCAAGTTTTCATCCATCAGTTCTCGTTTTAAGCTACAACTCCAACAATTGATGGAAATACTAAATTCTACAGAACCCCATTACATAAGATGTATAAAGCCGAATAATGTTTTAAAGCCCGCTGTATTTGAGAATGATAATGTCATGCAACAGCTACGTTCTGGTGGTGTTTTAGAGGCTGTCCGAATAAAATGTGCTGGATACCCTACTTACAGGAGTTTTTCTGAATTTTTAAGTCGATTCCGTGTCCTGGCCCCAGAAGTTTTAAAAATGGATTGTCCTGAAAATGAAGCCTGTGAAAAGATTTTGGTGAAGATGGGCCTTAGAGGTTATCAGATAGGGAAAACAAAGGTTTTCCTAAGAGCTGGTCAGATGGCTGAGCTAGATGCAAAGAGAATACTCTTACTTGGCGACTCATCTAAGGTGATTCAAACGCGGGGCAGGACTCGTATAACTCGTAAAAAGTATGTTTCTACACGGGAGGCTTCTATTTGTGTACAATCATTTTGTAAAGGAGAACTGGTTCGCAAGTTATTCAAGCTCAAGAAGCGGGAGATTTCCGCAGTTAAAATTCAGAAGACTGCCCGTAAACGCCTGGCAAGAAAAGACTATCTCAGAATCAAGTTCGCTTCGACTATCTTACAGGCAGGTGTAAGGGCAGTGGCTGCCCGTGATGAATTTAGATATAGGGTTAATGCGGCAATTATTATTCAGACAGGTTTACGGGCAATGGCTGCTCGTGATGTTTTTAGATGTAGAATTAATGCGGCAGTTATTATTCAGACAGGTTTACGTGCAATGGCTGCTCGTGATGTTTTTAGATGTAGAGCCCAATCTAAGCCTAAAGTTATTGTTCAGACTGATTTGGAGACAAAAATAGCAGAAACACCGAAGTTGGAGCCCTCCGTGCAAGCTAATACTGACTTGGAAAAAGAAAAGGTTGAAGAGGCAACAAATGCAGAGTCCTCCCTGCTTACTAATGTAAGCAGAGGGTTTGAAATAGATGCATTGTCTAAAAGTCAGGTTGCACAGGTAGAAATTGAAGATGCATGCTCTATAACCGAAGATCTTTCAAGTCCTGATCAAAACGCTGAAAGAGTTAAAATTCTTACTGCAGAAGTTGAAAGTCTGAAGGTCATGTTGCAAGCTGAAAAACAAAGAGCCAATGAGTGTGAAAGGAGATACGTAGAAGCTCGGGTATCAAGTGAGGAAGGACGTAGAAAGTTAGAGGAAACTGAAAGAATAGTTCTTCAACTTCAGGAATCTTTGAACAGGATGATATATTGCATGTCAAACCAAGTTTCCGAGCTGAAAACAATATTATCTACAGCATCCAAGTCAAGTTCAACAACTTCAGGACCTTTGGTTAGACACGAAGGGTTTGATAGCATTTCCAGTAACTCTGATTATTCATCCACCGACTCAGATTTCACTTTTCCAGCTCCAGCTACAAGTTCAGTTAACTTTTCTTCTACCGGCCCCAGTTCTATCCAGCTTATAGTTCAGGACGTTTCAGGCGGAGAAGTTTCAGGATCTGAAAACGAGAAGGAGGGGGCTTTTGATGACTTCTTCTGA

Protein Analysis

1162

Amino Acids

130.82

Weight (kDa)

8.37

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_head PF00063 97 - 758 5.6e-247 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2549
Acc36I ACCTGC 1 cut(s) 2549
AccBSI CCGCTC 1 cut(s) 1725
AccII CGCG 1 cut(s) 2347
AclWI GGATC 2 cut(s) 1259, 3451
AcoI YGGCCR 2 cut(s) 1299, 1902
AcsI RAATTY 8 cut(s) 369, 749, 1553, 1975, 2138, 2481, 2588, 3015
AcuI CTGAAG 4 cut(s) 666, 3062, 3152, 3249
AfaI GTAC 4 cut(s) 180, 468, 1776, 2409
AfiI CCNNNNNNNGG 3 cut(s) 284, 841, 2161
AflIII ACRYGT 1 cut(s) 1856
AhlI ACTAGT 1 cut(s) 215
AjnI CCWGG 3 cut(s) 280, 2160, 2505
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AloI GAACNNNNNNTCC 2 cut(s) 2418, 2450
Alw26I GTCTC 2 cut(s) 425, 2774
AlwI GGATC 2 cut(s) 1259, 3451
AlwNI CAGNNNCTG 2 cut(s) 2576, 3446
Ama87I CYCGRG 2 cut(s) 1194, 3105
AoxI GGCC 9 cut(s) 108, 485, 1299, 1761, 1873, 1902, 2163, 2230, 3388
ApoI RAATTY 8 cut(s) 369, 749, 1553, 1975, 2138, 2481, 2588, 3015
ArsI GACNNNNNNTTYG 2 cut(s) 2858, 2890
AseI ATTAAT 2 cut(s) 1452, 2673
Asp700I GAANNNNTTC 1 cut(s) 2433
AspLEI GCGC 2 cut(s) 612, 1277
AspS9I GGNCC 6 cut(s) 108, 1366, 2164, 2230, 3269, 3389
AsuC2I CCSGG 1 cut(s) 327
AsuHPI GGTGA 3 cut(s) 1726, 2233, 2347
AvaI CYCGRG 2 cut(s) 1194, 3105
AvaII GGWCC 2 cut(s) 1366, 3269
AxyI CCTNAGG 1 cut(s) 894
BalI TGGCCA 2 cut(s) 1301, 1904
BanII GRGCYC 3 cut(s) 1201, 2743, 2814
BauI CACGAG 3 cut(s) 825, 2649, 2718
BbsI GAAGAC 2 cut(s) 31, 2495
BccI CCATC 8 cut(s) 410, 442, 965, 1286, 1934, 1957, 2221, 2278
BciT130I CCWGG 3 cut(s) 282, 2162, 2507
BciVI GTATCC 2 cut(s) 259, 2105
BclI TGATCA 3 cut(s) 982, 1789, 2992
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 2 cut(s) 425, 2774
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 3 cut(s) 216, 351, 2294
BfmI CTRYAG 4 cut(s) 639, 1980, 3024, 3237
BfuAI ACCTGC 1 cut(s) 2549
BfuI GTATCC 2 cut(s) 259, 2105
BglII AGATCT 2 cut(s) 821, 2977
BlpI GCTNAGC 1 cut(s) 2289
BmcAI AGTACT 1 cut(s) 180
Bme1390I CCNGG 4 cut(s) 282, 327, 2162, 2507
Bme18I GGWCC 2 cut(s) 1366, 3269
BmeT110I CYCGRG 2 cut(s) 1194, 3105
BmgT120I GGNCC 6 cut(s) 108, 1366, 2164, 2230, 3269, 3389
BmiI GGNNCC 5 cut(s) 1368, 1770, 2166, 2811, 3391
BmrFI CCNGG 4 cut(s) 282, 327, 2162, 2507
BmrI ACTGGG 2 cut(s) 152, 3387
BmsI GCATC 8 cut(s) 427, 499, 1198, 1859, 2287, 2905, 2947, 3251
BmuI ACTGGG 2 cut(s) 152, 3387
BpiI GAAGAC 2 cut(s) 31, 2495
BpmI CTGGAG 2 cut(s) 1221, 3339
Bpu1102I GCTNAGC 1 cut(s) 2289
BpuEI CTTGAG 1 cut(s) 2438
BpuMI CCSGG 1 cut(s) 327
Bsa29I ATCGAT 1 cut(s) 1536
BsaAI YACGTR 2 cut(s) 2705, 3097
BsaJI CCNNGG 4 cut(s) 1014, 1193, 1270, 1764
BsaXI ACNNNNNCTCC 6 cut(s) 259, 289, 1358, 1388, 2418, 2448
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 841, 2161
Bse118I RCCGGY 1 cut(s) 3386
Bse1I ACTGG 8 cut(s) 116, 158, 388, 686, 1334, 2436, 3306, 3393
Bse21I CCTNAGG 1 cut(s) 894
Bse3DI GCAATG 2 cut(s) 2646, 2715
BseBI CCWGG 3 cut(s) 282, 2162, 2507
BseCI ATCGAT 1 cut(s) 1536
BseDI CCNNGG 4 cut(s) 1014, 1193, 1270, 1764
BseGI GGATG 6 cut(s) 1024, 1297, 1922, 3192, 3242, 3323
BseLI CCNNNNNNNGG 3 cut(s) 284, 841, 2161
BseMI GCAATG 2 cut(s) 2646, 2715
BseMII CTCAG 8 cut(s) 195, 473, 908, 1740, 1884, 2280, 2539, 3348
BseNI ACTGG 8 cut(s) 116, 158, 388, 686, 1334, 2436, 3306, 3393
BseRI GAGGAG 3 cut(s) 267, 866, 1910
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 2347
BshFI GGCC 9 cut(s) 110, 487, 1301, 1763, 1875, 1904, 2165, 2232, 3390
BshVI ATCGAT 1 cut(s) 1536
BsiHKCI CYCGRG 2 cut(s) 1194, 3105
BsiSI CCGG 2 cut(s) 327, 3387
BslI CCNNNNNNNGG 3 cut(s) 284, 841, 2161
BsmAI GTCTC 2 cut(s) 425, 2774
BsnI GGCC 9 cut(s) 110, 487, 1301, 1763, 1875, 1904, 2165, 2232, 3390
BsoBI CYCGRG 2 cut(s) 1194, 3105
Bsp1286I GDGCHC 3 cut(s) 1201, 2743, 2814
Bsp1407I TGTACA 2 cut(s) 1774, 2407
Bsp1720I GCTNAGC 1 cut(s) 2289
Bsp19I CCATGG 2 cut(s) 1014, 1270
BspANI GGCC 9 cut(s) 110, 487, 1301, 1763, 1875, 1904, 2165, 2232, 3390
BspCNI CTCAG 8 cut(s) 196, 474, 907, 1739, 1885, 2281, 2538, 3347
BspDI ATCGAT 1 cut(s) 1536
BspFNI CGCG 1 cut(s) 2347
BspHI TCATGA 1 cut(s) 1651
BspLI GGNNCC 5 cut(s) 1368, 1770, 2166, 2811, 3391
BspMAI CTGCAG 2 cut(s) 643, 3028
BspMI ACCTGC 1 cut(s) 2549
BspPI GGATC 2 cut(s) 1259, 3451
BsrBI CCGCTC 1 cut(s) 1725
BsrDI GCAATG 2 cut(s) 2646, 2715
BsrFI RCCGGY 1 cut(s) 3386
BsrGI TGTACA 2 cut(s) 1774, 2407
BsrI ACTGG 8 cut(s) 116, 158, 388, 686, 1334, 2436, 3306, 3393
BssAI RCCGGY 1 cut(s) 3386
BssECI CCNNGG 4 cut(s) 1014, 1193, 1270, 1764
BssSI CACGAG 3 cut(s) 825, 2649, 2718
BssT1I CCWWGG 3 cut(s) 1014, 1270, 1764
Bst2BI CACGAG 3 cut(s) 825, 2649, 2718
Bst2UI CCWGG 3 cut(s) 282, 2162, 2507
Bst4CI ACNGT 7 cut(s) 456, 658, 718, 866, 1215, 1282, 1433
Bst6I CTCTTC 1 cut(s) 2850
BstAPI GCANNNNNTGC 4 cut(s) 2576, 2645, 2714, 2867
BstAUI TGTACA 2 cut(s) 1774, 2407
BstBAI YACGTR 2 cut(s) 2705, 3097
BstC8I GCNNGC 5 cut(s) 1873, 2031, 2823, 2962, 3057
BstDSI CCRYGG 2 cut(s) 1014, 1270
BstF5I GGATG 6 cut(s) 1024, 1297, 1922, 3192, 3242, 3323
BstFNI CGCG 1 cut(s) 2347
BstHHI GCGC 2 cut(s) 612, 1277
BstMAI GTCTC 2 cut(s) 425, 2774
BstMWI GCNNNNNNNGC 8 cut(s) 28, 512, 2458, 2576, 2645, 2714, 2818, 2867
BstNI CCWGG 3 cut(s) 282, 2162, 2507
BstNSI RCATGY 4 cut(s) 1496, 1860, 2964, 3202
BstSCI CCNGG 4 cut(s) 280, 325, 2160, 2505
BstSFI CTRYAG 4 cut(s) 639, 1980, 3024, 3237
BstSNI TACGTA 1 cut(s) 3097
BstUI CGCG 1 cut(s) 2347
BstV2I GAAGAC 2 cut(s) 31, 2495
BstX2I RGATCY 3 cut(s) 821, 2977, 3443
BstYI RGATCY 3 cut(s) 821, 2977, 3443
Bsu15I ATCGAT 1 cut(s) 1536
Bsu36I CCTNAGG 1 cut(s) 894
BsuI GTATCC 2 cut(s) 259, 2105
BsuRI GGCC 9 cut(s) 110, 487, 1301, 1763, 1875, 1904, 2165, 2232, 3390
BsuTUI ATCGAT 1 cut(s) 1536
BtgI CCRYGG 2 cut(s) 1014, 1270
BtgZI GCGATG 1 cut(s) 382
BtsCI GGATG 6 cut(s) 1024, 1297, 1922, 3192, 3242, 3323
BtsI GCAGTG 1 cut(s) 2577
BtsIMutI CAGTG 5 cut(s) 123, 381, 862, 1211, 2577
BveI ACCTGC 1 cut(s) 2549
Cac8I GCNNGC 5 cut(s) 1873, 2031, 2823, 2962, 3057
CaiI CAGNNNCTG 2 cut(s) 2576, 3446
CciI TCATGA 1 cut(s) 1651
CfoI GCGC 2 cut(s) 612, 1277
Cfr10I RCCGGY 1 cut(s) 3386
Cfr13I GGNCC 6 cut(s) 108, 1366, 2164, 2230, 3269, 3389
ClaI ATCGAT 1 cut(s) 1536
CseI GACGC 2 cut(s) 209, 1513
Csp6I GTAC 4 cut(s) 179, 467, 1775, 2408
CviQI GTAC 4 cut(s) 179, 467, 1775, 2408
DraI TTTAAA 2 cut(s) 2025, 2178
EaeI YGGCCR 2 cut(s) 1299, 1902
Eam1104I CTCTTC 1 cut(s) 2850
EarI CTCTTC 1 cut(s) 2850
EciI GGCGGA 2 cut(s) 667, 3444
Eco105I TACGTA 1 cut(s) 3097
Eco130I CCWWGG 3 cut(s) 1014, 1270, 1764
Eco147I AGGCCT 2 cut(s) 487, 1875
Eco24I GRGCYC 3 cut(s) 1201, 2743, 2814
Eco47I GGWCC 2 cut(s) 1366, 3269
Eco57I CTGAAG 4 cut(s) 666, 3062, 3152, 3249
Eco81I CCTNAGG 1 cut(s) 894
Eco88I CYCGRG 2 cut(s) 1194, 3105
EcoO109I RGGNCCY 2 cut(s) 1366, 3269
EcoRII CCWGG 3 cut(s) 280, 2160, 2505
EcoT14I CCWWGG 3 cut(s) 1014, 1270, 1764
EcoT22I ATGCAT 2 cut(s) 2920, 2962
EcoT38I GRGCYC 3 cut(s) 1201, 2743, 2814
ErhI CCWWGG 3 cut(s) 1014, 1270, 1764
FalI AAGNNNNNCTT 2 cut(s) 1560, 1592
FauI CCCGC 4 cut(s) 1730, 2038, 2340, 2454
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 3 cut(s) 982, 1789, 2992
FokI GGATG 6 cut(s) 1031, 1304, 1909, 3199, 3229, 3310
FriOI GRGCYC 3 cut(s) 1201, 2743, 2814
FspBI CTAG 3 cut(s) 216, 351, 2294
GlaI GCGC 2 cut(s) 611, 1276
GsuI CTGGAG 2 cut(s) 1221, 3339
HaeIII GGCC 9 cut(s) 110, 487, 1301, 1763, 1875, 1904, 2165, 2232, 3390
HapII CCGG 2 cut(s) 327, 3387
HgaI GACGC 2 cut(s) 209, 1513
HhaI GCGC 2 cut(s) 612, 1277
Hin6I GCGC 2 cut(s) 610, 1275
HinP1I GCGC 2 cut(s) 610, 1275
HincII GTYRAC 1 cut(s) 3373
HindII GTYRAC 1 cut(s) 3373
HindIII AAGCTT 2 cut(s) 1417, 1626
HpaI GTTAAC 1 cut(s) 3373
HpaII CCGG 2 cut(s) 327, 3387
HphI GGTGA 3 cut(s) 1726, 2233, 2347
Hpy166II GTNNAC 5 cut(s) 2408, 2501, 2633, 2702, 3373
Hpy8I GTNNAC 5 cut(s) 2408, 2501, 2633, 2702, 3373
HpyAV CCTTC 6 cut(s) 73, 641, 3037, 3116, 3281, 3451
HpyCH4III ACNGT 7 cut(s) 456, 658, 718, 866, 1215, 1282, 1433
HpyCH4IV ACGT 5 cut(s) 2068, 2704, 3096, 3127, 3420
HpyF10VI GCNNNNNNNGC 8 cut(s) 28, 512, 2458, 2576, 2645, 2714, 2818, 2867
HpySE526I ACGT 5 cut(s) 2068, 2704, 3096, 3127, 3420
HspAI GCGC 2 cut(s) 610, 1275
Ksp22I TGATCA 3 cut(s) 982, 1789, 2992
KspAI GTTAAC 1 cut(s) 3373
LmnI GCTCC 4 cut(s) 575, 1542, 2809, 3358
LweI GCATC 8 cut(s) 427, 499, 1198, 1859, 2287, 2905, 2947, 3251
MaeI CTAG 3 cut(s) 216, 351, 2294
MaeII ACGT 5 cut(s) 2068, 2704, 3096, 3127, 3420
MaeIII GTNAC 5 cut(s) 118, 704, 854, 1231, 3308
MbiI CCGCTC 1 cut(s) 1725
MfeI CAATTG 2 cut(s) 1356, 1958
MflI RGATCY 3 cut(s) 821, 2977, 3443
MhlI GDGCHC 3 cut(s) 1201, 2743, 2814
MlsI TGGCCA 2 cut(s) 1301, 1904
MluNI TGGCCA 2 cut(s) 1301, 1904
MlyI GAGTC 5 cut(s) 1115, 2318, 2350, 2882, 3326
MmeI TCCRAC 3 cut(s) 1006, 1978, 2787
Mox20I TGGCCA 2 cut(s) 1301, 1904
Mph1103I ATGCAT 2 cut(s) 2920, 2962
MroXI GAANNNNTTC 1 cut(s) 2433
MscI TGGCCA 2 cut(s) 1301, 1904
MslI CAYNNNNRTG 2 cut(s) 432, 1855
Msp20I TGGCCA 2 cut(s) 1301, 1904
MspA1I CMGCKG 2 cut(s) 644, 2033
MspI CCGG 2 cut(s) 327, 3387
MspR9I CCNGG 4 cut(s) 282, 327, 2162, 2507
MunI CAATTG 2 cut(s) 1356, 1958
MvaI CCWGG 3 cut(s) 282, 2162, 2507
MvnI CGCG 1 cut(s) 2347
MwoI GCNNNNNNNGC 8 cut(s) 28, 512, 2458, 2576, 2645, 2714, 2818, 2867
NciI CCSGG 1 cut(s) 327
NcoI CCATGG 2 cut(s) 1014, 1270
NdeI CATATG 1 cut(s) 472
NlaIV GGNNCC 5 cut(s) 1368, 1770, 2166, 2811, 3391
NmuCI GTSAC 1 cut(s) 118
NsiI ATGCAT 2 cut(s) 2920, 2962
NspI RCATGY 4 cut(s) 1496, 1860, 2964, 3202
PaeI GCATGC 1 cut(s) 2964
PagI TCATGA 1 cut(s) 1651
PaqCI CACCTGC 1 cut(s) 2549
PceI AGGCCT 2 cut(s) 487, 1875
PciI ACATGT 1 cut(s) 1856
PcsI WCGNNNNNNNCGW 1 cut(s) 2365
PdmI GAANNNNTTC 1 cut(s) 2433
PleI GAGTC 5 cut(s) 1115, 2318, 2350, 2881, 3326
PpsI GAGTC 5 cut(s) 1115, 2318, 2350, 2881, 3326
Ppu21I YACGTR 2 cut(s) 2705, 3097
PpuMI RGGWCCY 2 cut(s) 1366, 3269
PscI ACATGT 1 cut(s) 1856
PshBI ATTAAT 2 cut(s) 1452, 2673
Psp5II RGGWCCY 2 cut(s) 1366, 3269
Psp6I CCWGG 3 cut(s) 280, 2160, 2505
PspGI CCWGG 3 cut(s) 280, 2160, 2505
PspN4I GGNNCC 5 cut(s) 1368, 1770, 2166, 2811, 3391
PspPI GGNCC 6 cut(s) 108, 1366, 2164, 2230, 3269, 3389
PspPPI RGGWCCY 2 cut(s) 1366, 3269
PstI CTGCAG 2 cut(s) 643, 3028
PstNI CAGNNNCTG 2 cut(s) 2576, 3446
PsuI RGATCY 3 cut(s) 821, 2977, 3443
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 4 cut(s) 180, 468, 1776, 2409
RsaNI GTAC 4 cut(s) 179, 467, 1775, 2408
RseI CAYNNNNRTG 2 cut(s) 432, 1855
Sau96I GGNCC 6 cut(s) 108, 1366, 2164, 2230, 3269, 3389
ScaI AGTACT 1 cut(s) 180
SchI GAGTC 5 cut(s) 1115, 2318, 2350, 2882, 3326
ScrFI CCNGG 4 cut(s) 282, 327, 2162, 2507
SduI GDGCHC 3 cut(s) 1201, 2743, 2814
SfaNI GCATC 8 cut(s) 427, 499, 1198, 1859, 2287, 2905, 2947, 3251
SfcI CTRYAG 4 cut(s) 639, 1980, 3024, 3237
SinI GGWCC 2 cut(s) 1366, 3269
SmiMI CAYNNNNRTG 2 cut(s) 432, 1855
SmlI CTYRAG 1 cut(s) 2453
SmoI CTYRAG 1 cut(s) 2453
SnaBI TACGTA 1 cut(s) 3097
SpeI ACTAGT 1 cut(s) 215
SphI GCATGC 1 cut(s) 2964
SseBI AGGCCT 2 cut(s) 487, 1875
SspI AATATT 2 cut(s) 1093, 3231
SspMI CTAG 3 cut(s) 216, 351, 2294
StuI AGGCCT 2 cut(s) 487, 1875
StyD4I CCNGG 4 cut(s) 280, 325, 2160, 2505
StyI CCWWGG 3 cut(s) 1014, 1270, 1764
TaaI ACNGT 7 cut(s) 456, 658, 718, 866, 1215, 1282, 1433
TaiI ACGT 5 cut(s) 2071, 2707, 3099, 3130, 3423
TaqI TCGA 7 cut(s) 551, 756, 1536, 1834, 1853, 2149, 2543
TaqII GACCGA 1 cut(s) 602
TatI WGTACW 3 cut(s) 178, 1774, 2407
TauI GCSGC 3 cut(s) 741, 2612, 2681
TscAI CASTG 5 cut(s) 123, 388, 869, 1218, 2577
TseFI GTSAC 1 cut(s) 118
Tsp45I GTSAC 1 cut(s) 118
TspGWI ACGGA 3 cut(s) 186, 2144, 2806
TspRI CASTG 5 cut(s) 123, 388, 869, 1218, 2577
VpaK11BI GGWCC 2 cut(s) 1366, 3269
VspI ATTAAT 2 cut(s) 1452, 2673
XapI RAATTY 8 cut(s) 369, 749, 1553, 1975, 2138, 2481, 2588, 3015
XceI RCATGY 4 cut(s) 1496, 1860, 2964, 3202
XmnI GAANNNNTTC 1 cut(s) 2433
XspI CTAG 3 cut(s) 216, 351, 2294
ZrmI AGTACT 1 cut(s) 180
Zsp2I ATGCAT 2 cut(s) 2920, 2962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.