Rroxscaffold_7G00179750
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003080
Physical Location & Seq
Forward (+)
19244012 .. 19254334
10323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179750.1

Sequence Viewer

Length: 2310 bp
ATGACTGTTATTGGAATAAGCGAAGTAGAACAGGAAGCTATTTTCAGAGTTGTGGCAGCAATTCTTCATCTTGGAAATATTGATTTTGCCAATGGGGAAGATCATGATTCATCCGTCATAAAAGATGATCAATCCCTTTTTCATCTTCAAATGGCAGCAGAACTTCTCATGTGTAATCCTCGAGCACTGGAAGATGCACTCTGTAAGCGTGTAATGATTACCCCAGAAGAAATTATTAAACGAAGTCTTGATCCACTTGGTGCAACAGTTAGCAGAGATGGTTTAGCCAAGACAATTTATTCTCAATTGTTTAACTGGTTGGTGGATAAAATCAATGTCTCAATTGGGCAAGACCCTAGCTCAAAATGTTTGATTGGGGTCCTTGATATATATGGTTTTGAAAGCTTCAAAACTAATAGTTTTGAGCAGTTTTGTATTAATTACACGAATGAAAAGCTGCAACAACATTTCAACAAGCATGTCTTCAAGATGGAACAAGAGGAATATACCAAAGAGGAAATTGACTGGAGCTACATAGAATTTGTTGATAACAAAGATGTCCTAGATCTCATTGAACAGAAAAAAGGTGGGATCATTGCTCTGCTTGATGAAGCCTGTATGTTTCCGAAATCAACTCATGAAACATTTTCAACAAAGCTTTATCAGACATTTAAAAATAATAAACGCTTCATCAAGCCAAAGCTGACCCGGTCAGATTTCACCGTTGTTCATTACGCAGGAGAAGTACAATATCAATCCGATCAATTTTTAGACAAAAATAAAGATTATGTTGTTCCTGAACATCGAGATTTGTTGAGTGCTTCAAAATGCCCCTTTGTAGTGGGTCTTTTCCCTCCGGTTTCTGAGGAGGCAGCCAAATCTTCGAAGTTCTCATCTATCAGTTCGCGTTTTAGGCTACAACTGCAACAATTGATGGAAACTCTAAATTCTACAGAGCCCCACTACATAAGATGTATAAAGCCAAATAATCTCTTAAAACCTGCAGTATTTGAAAATGTCAGTGTCATCCAGCAACTACGTTCTGGTGGTGTTTTAGAGGCAGTCAGAATAAAATGTGCTGGATTCCCTACTTACAGGAATTTTTCAGAATTTTTAACTCGTTTTCGTATCCTGGCACCAGAGGTCTTGAAAATGGAGTGTTCTGAAAATGAAGCCTGTAAAAAGATTTTGGAGAAGATGGGGCTTAAGGATTATCAGATAGGGAAAACAAAGGTTTTTTTAAGAGTTGGTCAGATGGCTGTGCTTGATGCACAGAGAACACTCATGCATGGCAACTCAGCGAAGGTGATTCAAACACGGGGCAGAACTCTTGTTACTCGTAAAAAGTTTGTTAGTGCACAGATGGCTTCTATTTGCATACAATCAGTTTGTAGAGGAGAATTGGCTCGCAAGTTATATAAGCTGAAGAAACAGGAGTTTGCCGCTGTTAAAATTCAGAAGACTTCACGTAAACGCCTGGCGAGAAAAGATTATCTCAGAATGATCTTCTCTTCAGTTCTCTTACAAGCACATCTACGGGCAATGGTTGCTCGTGATGATTTTAAATCCAAACGTTCGGCAGCAGTTACTATTCAGTCAGGTTTACGTGCAATGGCTGCTCGTGATGATCTTAGATATAAAGTAGAGGCTGCAGTTGTTATTCAGACAGGTTTAAGGGCAATTGCTGCTCGTGATACCTATAGAAAAAGAGCAAAATCCAGTGCAGCAGTTATTACTCAGACTGACTTGGAAGAACCAAAGGAAAAAGAAGCGACAAAGTCAGAGGCCTCTAAGCAAGCTAAAACTGACTTGGAAGAAGCAAAAGGAGAAGAGGCAACAAAGTTGGAGTCCTCTGTGAAAGATAAGAATGGCCCCAAAGAAGCAAAAGGAGAAGAGTCAACAAAGTTGGAGTCCTCTGTGAAAGTTGATGAAAAAAGTGTGTTGGTTGAGAGTCGGGCTGCCGTGATAGAAATTGACAAAACATCCCCTATGATCCAAAAACTTCCTGGTCCAGATCAAGATGCTGACATAATTAAGATTCTTACTGCAGAAGTCGAAAATTTGAAGGTCATGTTGCAAGCAGAAAAACAGAGAGCTAATGAGTTTGAAAGGAGATATCTAGAAGCCCGGGTATCAAGCGAGCAAGGACATAAAAAATTAGAGGAAACTGAAAGAATAGTACATCAACTTCAGGACTCTTTGAACAGTGTGTTTCAGGATGATATATTGCATGTCGAACCAAGTTTCAGAGCTCAAAACAATATTGTGTACTGCATCAAAATCAAGTTCGGCTTCTGGGCCTCTTGTTAG

Protein Analysis

769

Amino Acids

87.38

Weight (kDa)

8.86

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_head PF00063 1 - 415 1.5e-141 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1009
AccB1I GGYRCC 1 cut(s) 1135
AccII CGCG 1 cut(s) 907
AciI CCGC 1 cut(s) 1443
AclI AACGTT 1 cut(s) 1573
AclWI GGATC 3 cut(s) 245, 599, 1987
AcsI RAATTY 6 cut(s) 539, 946, 1099, 1109, 1452, 2059
AcuI CTGAAG 3 cut(s) 1445, 1497, 2174
AdeI CACNNNGTG 1 cut(s) 260
AfaI GTAC 3 cut(s) 747, 2181, 2270
AflII CTTAAG 1 cut(s) 1205
AjnI CCWGG 3 cut(s) 1131, 1476, 2005
Alw21I GWGCWC 3 cut(s) 187, 1360, 2254
Alw26I GTCTC 1 cut(s) 343
Alw44I GTGCAC 1 cut(s) 1356
AlwI GGATC 3 cut(s) 245, 599, 1987
Ama87I CYCGRG 2 cut(s) 180, 2127
AoxI GGCC 3 cut(s) 1785, 1870, 2298
ApaLI GTGCAC 1 cut(s) 1356
ApoI RAATTY 6 cut(s) 539, 946, 1099, 1109, 1452, 2059
ArsI GACNNNNNNTTYG 4 cut(s) 1832, 1864, 1895, 1927
AseI ATTAAT 1 cut(s) 438
AspS9I GGNCC 4 cut(s) 379, 1871, 2009, 2298
AsuC2I CCSGG 3 cut(s) 709, 2128, 2129
AsuHPI GGTGA 2 cut(s) 712, 1318
AsuII TTCGAA 1 cut(s) 884
AvaI CYCGRG 2 cut(s) 180, 2127
AvaII GGWCC 2 cut(s) 379, 2009
BaeGI GKGCMC 1 cut(s) 1360
BanI GGYRCC 1 cut(s) 1135
BanII GRGCYC 2 cut(s) 960, 2254
BarI GAAGNNNNNNTAC 2 cut(s) 2172, 2204
BauI CACGAG 3 cut(s) 1551, 1620, 1689
BbsI GAAGAC 2 cut(s) 475, 1466
Bbv12I GWGCWC 3 cut(s) 187, 1360, 2254
BccI CCATC 6 cut(s) 272, 484, 928, 1192, 1249, 1357
BceAI ACGGC 1 cut(s) 1946
BciT130I CCWGG 3 cut(s) 1133, 1478, 2007
BciVI GTATCC 1 cut(s) 1139
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 3 cut(s) 709, 2128, 2129
BcoDI GTCTC 1 cut(s) 343
BfaI CTAG 3 cut(s) 357, 563, 2120
BfmI CTRYAG 5 cut(s) 951, 1002, 1650, 1699, 2046
BfrI CTTAAG 1 cut(s) 1205
BfuAI ACCTGC 1 cut(s) 1009
BfuI GTATCC 1 cut(s) 1139
BglII AGATCT 1 cut(s) 565
Bme1390I CCNGG 6 cut(s) 709, 1133, 1478, 2007, 2128, 2129
Bme18I GGWCC 2 cut(s) 379, 2009
BmeT110I CYCGRG 2 cut(s) 180, 2127
BmgT120I GGNCC 4 cut(s) 379, 1871, 2009, 2298
BmiI GGNNCC 3 cut(s) 380, 1137, 1873
BmrFI CCNGG 6 cut(s) 709, 1133, 1478, 2007, 2128, 2129
BmsI GCATC 4 cut(s) 184, 1258, 2011, 2283
BpiI GAAGAC 2 cut(s) 475, 1466
BpmI CTGGAG 1 cut(s) 547
Bpu14I TTCGAA 1 cut(s) 884
BpuMI CCSGG 3 cut(s) 709, 2128, 2129
BsaAI YACGTR 2 cut(s) 1469, 1607
BsaJI CCNNGG 1 cut(s) 2127
BsaWI WCCGGW 1 cut(s) 856
Bse1I ACTGG 4 cut(s) 192, 320, 530, 1719
Bse3DI GCAATG 3 cut(s) 594, 1548, 1617
BseBI CCWGG 3 cut(s) 1133, 1478, 2007
BseDI CCNNGG 1 cut(s) 2127
BseGI GGATG 4 cut(s) 110, 1026, 1982, 2224
BseMI GCAATG 3 cut(s) 594, 1548, 1617
BseMII CTCAG 4 cut(s) 855, 1311, 1510, 1751
BseNI ACTGG 4 cut(s) 192, 320, 530, 1719
BseRI GAGGAG 2 cut(s) 881, 1410
BseSI GKGCMC 1 cut(s) 1360
BsgI GTGCAG 1 cut(s) 1743
Bsh1236I CGCG 1 cut(s) 907
BshFI GGCC 3 cut(s) 1787, 1872, 2300
BshNI GGYRCC 1 cut(s) 1135
BsiHKAI GWGCWC 3 cut(s) 187, 1360, 2254
BsiHKCI CYCGRG 2 cut(s) 180, 2127
BsiSI CCGG 3 cut(s) 709, 857, 2128
BsmAI GTCTC 1 cut(s) 343
BsnI GGCC 3 cut(s) 1787, 1872, 2300
BsoBI CYCGRG 2 cut(s) 180, 2127
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 4 cut(s) 187, 960, 1360, 2254
BspACI CCGC 1 cut(s) 1443
BspANI GGCC 3 cut(s) 1787, 1872, 2300
BspCNI CTCAG 4 cut(s) 856, 1310, 1509, 1750
BspFNI CGCG 1 cut(s) 907
BspHI TCATGA 2 cut(s) 103, 637
BspLI GGNNCC 3 cut(s) 380, 1137, 1873
BspMAI CTGCAG 3 cut(s) 1006, 1654, 2050
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 3 cut(s) 245, 599, 1987
BspT104I TTCGAA 1 cut(s) 884
BspT107I GGYRCC 1 cut(s) 1135
BspTI CTTAAG 1 cut(s) 1205
BsrDI GCAATG 3 cut(s) 594, 1548, 1617
BsrI ACTGG 4 cut(s) 192, 320, 530, 1719
BssECI CCNNGG 1 cut(s) 2127
BssSI CACGAG 3 cut(s) 1551, 1620, 1689
Bst2BI CACGAG 3 cut(s) 1551, 1620, 1689
Bst2UI CCWGG 3 cut(s) 1133, 1478, 2007
Bst4CI ACNGT 4 cut(s) 7, 268, 724, 2207
Bst6I CTCTTC 3 cut(s) 1516, 1824, 1887
BstAFI CTTAAG 1 cut(s) 1205
BstAPI GCANNNNNTGC 3 cut(s) 1547, 1616, 1685
BstBAI YACGTR 2 cut(s) 1469, 1607
BstBI TTCGAA 1 cut(s) 884
BstC8I GCNNGC 4 cut(s) 1408, 1797, 2079, 2141
BstDEI CTNAG 6 cut(s) 864, 1297, 1496, 1631, 1737, 1791
BstF5I GGATG 4 cut(s) 110, 1026, 1982, 2224
BstFNI CGCG 1 cut(s) 907
BstMAI GTCTC 1 cut(s) 343
BstMWI GCNNNNNNNGC 6 cut(s) 913, 922, 1364, 1547, 1616, 1685
BstNI CCWGG 3 cut(s) 1133, 1478, 2007
BstNSI RCATGY 2 cut(s) 482, 2234
BstSCI CCNGG 6 cut(s) 707, 1131, 1476, 2005, 2126, 2127
BstSFI CTRYAG 5 cut(s) 951, 1002, 1650, 1699, 2046
BstSLI GKGCMC 1 cut(s) 1360
BstUI CGCG 1 cut(s) 907
BstV2I GAAGAC 2 cut(s) 475, 1466
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BsuI GTATCC 1 cut(s) 1139
BsuRI GGCC 3 cut(s) 1787, 1872, 2300
BtsCI GGATG 4 cut(s) 110, 1026, 1982, 2224
BtsIMutI CAGTG 4 cut(s) 185, 1027, 1726, 2212
BveI ACCTGC 1 cut(s) 1009
Cac8I GCNNGC 4 cut(s) 1408, 1797, 2079, 2141
CciI TCATGA 2 cut(s) 103, 637
Cfr13I GGNCC 4 cut(s) 379, 1871, 2009, 2298
Cfr9I CCCGGG 1 cut(s) 2127
Csp6I GTAC 3 cut(s) 746, 2180, 2269
CviAII CATG 8 cut(s) 104, 169, 479, 638, 1285, 1289, 2071, 2231
CviQI GTAC 3 cut(s) 746, 2180, 2269
DdeI CTNAG 6 cut(s) 864, 1297, 1496, 1631, 1737, 1791
DraI TTTAAA 2 cut(s) 673, 1564
DraIII CACNNNGTG 1 cut(s) 260
Eam1104I CTCTTC 3 cut(s) 1516, 1824, 1887
EarI CTCTTC 3 cut(s) 1516, 1824, 1887
Ecl136II GAGCTC 1 cut(s) 2252
Eco147I AGGCCT 1 cut(s) 1787
Eco24I GRGCYC 2 cut(s) 960, 2254
Eco32I GATATC 1 cut(s) 2117
Eco47I GGWCC 2 cut(s) 379, 2009
Eco53kI GAGCTC 1 cut(s) 2252
Eco57I CTGAAG 3 cut(s) 1445, 1497, 2174
Eco88I CYCGRG 2 cut(s) 180, 2127
EcoICRI GAGCTC 1 cut(s) 2252
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 3 cut(s) 1131, 1476, 2005
EcoRV GATATC 1 cut(s) 2117
EcoT22I ATGCAT 1 cut(s) 1290
EcoT38I GRGCYC 2 cut(s) 960, 2254
FaeI CATG 8 cut(s) 107, 172, 482, 641, 1288, 1292, 2074, 2234
FalI AAGNNNNNCTT 4 cut(s) 467, 499, 2276, 2308
FatI CATG 8 cut(s) 103, 168, 478, 637, 1284, 1288, 2070, 2230
FbaI TGATCA 1 cut(s) 127
FokI GGATG 4 cut(s) 97, 1013, 1969, 2231
FriOI GRGCYC 2 cut(s) 960, 2254
FspBI CTAG 3 cut(s) 357, 563, 2120
GsuI CTGGAG 1 cut(s) 547
HaeIII GGCC 3 cut(s) 1787, 1872, 2300
HapII CCGG 3 cut(s) 709, 857, 2128
Hin1II CATG 8 cut(s) 107, 172, 482, 641, 1288, 1292, 2074, 2234
HincII GTYRAC 1 cut(s) 1899
HindII GTYRAC 1 cut(s) 1899
HindIII AAGCTT 2 cut(s) 403, 656
HinfI GANTC 9 cut(s) 107, 1083, 1309, 1847, 1895, 1910, 1951, 2038, 2195
HpaII CCGG 3 cut(s) 709, 857, 2128
HphI GGTGA 2 cut(s) 712, 1318
Hpy166II GTNNAC 5 cut(s) 1358, 1472, 1604, 1899, 2269
Hpy8I GTNNAC 5 cut(s) 1358, 1472, 1604, 1899, 2269
HpyAV CCTTC 2 cut(s) 1297, 2059
HpyCH4III ACNGT 4 cut(s) 7, 268, 724, 2207
HpyCH4IV ACGT 4 cut(s) 1039, 1468, 1573, 1606
HpyF10VI GCNNNNNNNGC 6 cut(s) 913, 922, 1364, 1547, 1616, 1685
HpyF3I CTNAG 6 cut(s) 864, 1297, 1496, 1631, 1737, 1791
HpySE526I ACGT 4 cut(s) 1039, 1468, 1573, 1606
Hsp92II CATG 8 cut(s) 107, 172, 482, 641, 1288, 1292, 2074, 2234
Ksp22I TGATCA 1 cut(s) 127
LmnI GCTCC 1 cut(s) 528
LweI GCATC 4 cut(s) 184, 1258, 2011, 2283
MaeI CTAG 3 cut(s) 357, 563, 2120
MaeII ACGT 4 cut(s) 1039, 1468, 1573, 1606
MaeIII GTNAC 2 cut(s) 1333, 1585
MfeI CAATTG 4 cut(s) 305, 342, 929, 1680
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 4 cut(s) 187, 960, 1360, 2254
MlyI GAGTC 5 cut(s) 1856, 1904, 1919, 1960, 2189
MmeI TCCRAC 2 cut(s) 1824, 1887
Mph1103I ATGCAT 1 cut(s) 1290
MspA1I CMGCKG 1 cut(s) 1445
MspCI CTTAAG 1 cut(s) 1205
MspI CCGG 3 cut(s) 709, 857, 2128
MspR9I CCNGG 6 cut(s) 709, 1133, 1478, 2007, 2128, 2129
MunI CAATTG 4 cut(s) 305, 342, 929, 1680
MvaI CCWGG 3 cut(s) 1133, 1478, 2007
MvnI CGCG 1 cut(s) 907
MwoI GCNNNNNNNGC 6 cut(s) 913, 922, 1364, 1547, 1616, 1685
NciI CCSGG 3 cut(s) 709, 2128, 2129
NlaIII CATG 8 cut(s) 107, 172, 482, 641, 1288, 1292, 2074, 2234
NlaIV GGNNCC 3 cut(s) 380, 1137, 1873
NsiI ATGCAT 1 cut(s) 1290
NspI RCATGY 2 cut(s) 482, 2234
NspV TTCGAA 1 cut(s) 884
PaeR7I CTCGAG 1 cut(s) 180
PagI TCATGA 2 cut(s) 103, 637
PceI AGGCCT 1 cut(s) 1787
PfeI GAWTC 4 cut(s) 107, 1083, 1309, 2038
PflFI GACNNNGTC 2 cut(s) 709, 1777
PleI GAGTC 5 cut(s) 1855, 1903, 1918, 1959, 2189
PpsI GAGTC 5 cut(s) 1855, 1903, 1918, 1959, 2189
Ppu21I YACGTR 2 cut(s) 1469, 1607
PpuMI RGGWCCY 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 2254
Psp1406I AACGTT 1 cut(s) 1573
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 3 cut(s) 1131, 1476, 2005
PspGI CCWGG 3 cut(s) 1131, 1476, 2005
PspN4I GGNNCC 3 cut(s) 380, 1137, 1873
PspPI GGNCC 4 cut(s) 379, 1871, 2009, 2298
PspPPI RGGWCCY 1 cut(s) 379
PspXI VCTCGAGB 1 cut(s) 180
PsrI GAACNNNNNNTAC 2 cut(s) 1318, 1350
PstI CTGCAG 3 cut(s) 1006, 1654, 2050
PsuI RGATCY 1 cut(s) 565
PsyI GACNNNGTC 2 cut(s) 709, 1777
RsaI GTAC 3 cut(s) 747, 2181, 2270
RsaNI GTAC 3 cut(s) 746, 2180, 2269
SacI GAGCTC 1 cut(s) 2254
Sau96I GGNCC 4 cut(s) 379, 1871, 2009, 2298
SchI GAGTC 5 cut(s) 1856, 1904, 1919, 1960, 2189
ScrFI CCNGG 6 cut(s) 709, 1133, 1478, 2007, 2128, 2129
SduI GDGCHC 4 cut(s) 187, 960, 1360, 2254
SfaNI GCATC 4 cut(s) 184, 1258, 2011, 2283
SfcI CTRYAG 5 cut(s) 951, 1002, 1650, 1699, 2046
Sfr274I CTCGAG 1 cut(s) 180
SfuI TTCGAA 1 cut(s) 884
SinI GGWCC 2 cut(s) 379, 2009
SlaI CTCGAG 1 cut(s) 180
SmaI CCCGGG 1 cut(s) 2129
SmlI CTYRAG 2 cut(s) 180, 1205
SmoI CTYRAG 2 cut(s) 180, 1205
SseBI AGGCCT 1 cut(s) 1787
SsiI CCGC 1 cut(s) 1443
SspI AATATT 2 cut(s) 79, 2263
SspMI CTAG 3 cut(s) 357, 563, 2120
SstI GAGCTC 1 cut(s) 2254
StuI AGGCCT 1 cut(s) 1787
StyD4I CCNGG 6 cut(s) 707, 1131, 1476, 2005, 2126, 2127
TaaI ACNGT 4 cut(s) 7, 268, 724, 2207
TaiI ACGT 4 cut(s) 1042, 1471, 1576, 1609
TaqI TCGA 5 cut(s) 181, 805, 884, 2055, 2235
TatI WGTACW 3 cut(s) 745, 2179, 2268
TauI GCSGC 1 cut(s) 1445
TfiI GAWTC 4 cut(s) 107, 1083, 1309, 2038
TscAI CASTG 4 cut(s) 192, 1027, 1726, 2212
TspGWI ACGGA 1 cut(s) 103
TspMI CCCGGG 1 cut(s) 2127
TspRI CASTG 4 cut(s) 192, 1027, 1726, 2212
Tth111I GACNNNGTC 2 cut(s) 709, 1777
Vha464I CTTAAG 1 cut(s) 1205
VneI GTGCAC 1 cut(s) 1356
VpaK11BI GGWCC 2 cut(s) 379, 2009
VspI ATTAAT 1 cut(s) 438
XapI RAATTY 6 cut(s) 539, 946, 1099, 1109, 1452, 2059
XbaI TCTAGA 1 cut(s) 2119
XceI RCATGY 2 cut(s) 482, 2234
XcmI CCANNNNNNNNNTGG 1 cut(s) 2003
XhoI CTCGAG 1 cut(s) 180
XmaI CCCGGG 1 cut(s) 2127
XspI CTAG 3 cut(s) 357, 563, 2120
Zsp2I ATGCAT 1 cut(s) 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.