MD05G1215600.v1.1

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003776
Physical Location & Seq
Reverse (-)
34656330 .. 34662729
6400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1215600.v1.1.491

Sequence Viewer

Length: 1815 bp
ATGTCCAGCAATGGCCGCAAGTACTCGTGCGAAACGGCGAGCCAGACCCTGGAATGGATCAACGCCATCGTCGACTTCATAAGACCCTACACTTTCCTAACCAACGCCCACGTCGTCAATTTCTTCAAGGACCGGCTGTGGGAAGCTGTCGACGAGGAATGGATGGACTGCTTGCGCAAAGAAGCAGTGGAGAATCTCCTGCAGATTCCTTGTGGAGTGGTCCAGGATCATTGGCCTGCTTCGCTTAAGAATTTTATTGTCACTTTGAGCTCTCTTGTGCTTCCTCGACAACAAGCAGACTTGCAGACGGTATTACCTGGCATGGACATGACTTCACTCAATAGCGTTCTCTCCACAGGCATGAATGCGAAGAAAAAACATGAAGTAGAAGTCCTTTCTGCGGTTGTTAATTCTATTGCTAGCAGTGTCAGGGCTAATGCAATAATTGATGTTGGCTCTGGTCAGGGGTATCTTGCACAAGTACTTTCCTTTCACTACCAACATGCAGTATTTGCAATTGATGCATGTTCTCATCATGGAAGGGTTACTGATGCACGTGCAGAACAAATCAAGAAATATTATGCTTCTCAAATCCGTAAATCTGTATCAGAAAACAGGAGTTTGACTGTGCCTAAGACAATCACATGCAATGTGATGTCTATTGACACGTTGAAAGCTTTGGCTGACATACCACTGGATGAAGATAATGTCTCTTCATTACAAACTTCCTGCAAGGGAGACAGTGGAACTTCACTGGTTCTTGCTGGCCTTCATGCTTGCGGAGATCTTTCAGTAACAATGCTCAAGACCTTTATGGAGTGCAAAGAAGTTAAAGCAGTTGTTAGCGTTGGCTGCTGTTACAACCTATTATCTGAAGAAGGTTCCAACCATGTGGGTTCTCAATGTGGTTTTCCAATGAGTCATGGTGTTAAATCTGCTGGTTTGTCACTTGGAAAAAGTTCACGTGACCTTGCATGTCAGAGTGCGGAGAGGTGGAGATGCTTGGAAAATGATGCTGGCGTCCATAACTTTGAGCTGCATGCTTTCCGTGCTGCTTTTCAGATGGTTCTCAGTGTATATTATCCCAAAGTGATGACTACAAGTCCATCAATTGGGCGTCAAGGAAAGGCTTTGCGTCGCCGACAGCAGAGGGTCGCCCCAGACTCCTCGCTGCATCATAAAGAAAGCACATGTTCATCCCTTTCCCAAATAAACTCTGCTATGAAAGGAAGCTGGTCTCACATAAAACCTACAGTACAGGAAGCAGATGGCAGTTTTGACACGGTGTTAGAGACAACGACCATAACTAATGAAAATTCCTTCAAGGCCACCCAATGTGGGAGGAGCAAGTTCATCGGCAAGTATCCACTTTTCCAGAAATATTGCTTATCTGGTTTATCTCGCCTTGGTCTTGAGCCTCTGAAGGAAATGGATGTTCATGGAATATGGAAGGAAGTTGAATCATCTGCTGAACTTATTGGACCTTATTGGTCTCTTCGAGCTGCTTTCGGGCCTCTTTTAGAAACTTTCCTTTTGCTTGATAGATTGTTGTTCCTCCAAGAGCAAGGGAGTTCAATTGAAGCAGAAATGCAACCAATTTTTAATCCTGCGTTGTCCCCAAGGAATGTGGCCATTATTGCTAAAAAGTTGACGACACAGATGAGGAGATCCAGATCCGACAACTTATGCAACGCAGTTGCAAGAACGTGTCGATGCATCATCATTACTGAAATTATTGCCAAAGGCAGAAGATGTGTTGAAATGTGCAGTCTCGGGAGAAGAACTCAAGGACTTGCTTGTACTGGATGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_32

Protein Analysis

605

Amino Acids

66.69

Weight (kDa)

8.45

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_32 PF13679 124 - 292 8.5e-27 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 176
AccB7I CCANNNNNTGG 2 cut(s) 49, 1112
AccI GTMKAC 2 cut(s) 72, 150
AciI CCGC 4 cut(s) 16, 401, 780, 986
AclWI GGATC 4 cut(s) 65, 234, 1662, 1668
AcoI YGGCCR 2 cut(s) 13, 1629
AcsI RAATTY 2 cut(s) 250, 1315
AcuI CTGAAG 2 cut(s) 894, 1442
AcvI CACGTG 2 cut(s) 557, 965
AcyI GRCGYC 2 cut(s) 1020, 1117
AfaI GTAC 4 cut(s) 23, 483, 1257, 1801
AfiI CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1338
AflII CTTAAG 1 cut(s) 245
AflIII ACRYGT 3 cut(s) 666, 1190, 1706
AgsI TTSAA 7 cut(s) 127, 673, 1324, 1460, 1575, 1580, 1760
AhdI GACNNNNNGTC 1 cut(s) 1101
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 3 cut(s) 48, 222, 316
AluBI AGCT 6 cut(s) 146, 270, 677, 1036, 1233, 1502
AluI AGCT 6 cut(s) 146, 270, 677, 1036, 1233, 1502
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 6 cut(s) 715, 732, 1242, 1286, 1497, 1775
AlwI GGATC 4 cut(s) 65, 234, 1662, 1668
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 1772
AoxI GGCC 6 cut(s) 13, 233, 766, 1326, 1511, 1629
ApeKI GCWGC 5 cut(s) 852, 1036, 1052, 1171, 1502
ApoI RAATTY 2 cut(s) 250, 1315
Asp700I GAANNNNTTC 1 cut(s) 958
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 4 cut(s) 130, 220, 1481, 1511
AsuNHI GCTAGC 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 1772
AvaII GGWCC 3 cut(s) 130, 220, 1481
BalI TGGCCA 1 cut(s) 1631
BanII GRGCYC 1 cut(s) 272
BauI CACGAG 1 cut(s) 25
BbrPI CACGTG 2 cut(s) 557, 965
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 5 cut(s) 839, 1023, 1039, 1158, 1489
BccI CCATC 6 cut(s) 74, 157, 1057, 1114, 1262, 1800
BceAI ACGGC 1 cut(s) 51
BcgI CGANNNNNNTGC 2 cut(s) 1336, 1370
BciT130I CCWGG 3 cut(s) 50, 224, 318
BciVI GTATCC 1 cut(s) 1374
BcoDI GTCTC 6 cut(s) 715, 732, 1242, 1286, 1497, 1775
BfaI CTAG 1 cut(s) 420
BfmI CTRYAG 2 cut(s) 200, 1251
BfrI CTTAAG 1 cut(s) 245
BfuI GTATCC 1 cut(s) 1374
BglII AGATCT 1 cut(s) 784
BisI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1503
BlsI GCNGC 6 cut(s) 17, 854, 1038, 1054, 1173, 1504
BmcAI AGTACT 2 cut(s) 23, 483
Bme1390I CCNGG 3 cut(s) 50, 224, 318
Bme18I GGWCC 3 cut(s) 130, 220, 1481
BmeRI GACNNNNNGTC 1 cut(s) 1101
BmeT110I CYCGRG 1 cut(s) 1772
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 130, 220, 1481, 1511
BmiI GGNNCC 1 cut(s) 883
BmrFI CCNGG 3 cut(s) 50, 224, 318
BmsI GCATC 7 cut(s) 511, 541, 989, 1003, 1183, 1703, 1725
BmtI GCTAGC 1 cut(s) 423
BplI GAGNNNNNCTC 2 cut(s) 1768, 1800
BpuEI CTTGAG 3 cut(s) 788, 1433, 1770
BsaAI YACGTR 2 cut(s) 557, 965
BsaBI GATNNNNATC 1 cut(s) 1672
BsaHI GRCGYC 2 cut(s) 1020, 1117
BsaI GGTCTC 2 cut(s) 1242, 1497
BsaJI CCNNGG 3 cut(s) 48, 1405, 1619
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1338
Bse118I RCCGGY 1 cut(s) 132
Bse1I ACTGG 3 cut(s) 699, 759, 1807
Bse3DI GCAATG 2 cut(s) 16, 655
Bse8I GATNNNNATC 1 cut(s) 1672
BseBI CCWGG 3 cut(s) 50, 224, 318
BseDI CCNNGG 3 cut(s) 48, 1405, 1619
BseGI GGATG 6 cut(s) 168, 703, 1196, 1438, 1811, 1815
BseJI GATNNNNATC 1 cut(s) 1672
BseLI CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1338
BseMI GCAATG 2 cut(s) 16, 655
BseMII CTCAG 1 cut(s) 1084
BseNI ACTGG 3 cut(s) 699, 759, 1807
BseRI GAGGAG 3 cut(s) 1156, 1357, 1678
BseXI GCAGC 5 cut(s) 839, 1023, 1039, 1158, 1489
BsgI GTGCAG 2 cut(s) 579, 1786
BshFI GGCC 6 cut(s) 15, 235, 768, 1328, 1513, 1631
BsiHKAI GWGCWC 1 cut(s) 272
BsiHKCI CYCGRG 1 cut(s) 1772
BsiSI CCGG 1 cut(s) 133
BslFI GGGAC 1 cut(s) 1600
BslI CCNNNNNNNGG 6 cut(s) 49, 54, 139, 400, 1112, 1338
BsmAI GTCTC 6 cut(s) 715, 732, 1242, 1286, 1497, 1775
BsmFI GGGAC 1 cut(s) 1600
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 6 cut(s) 15, 235, 768, 1328, 1513, 1631
Bso31I GGTCTC 2 cut(s) 1242, 1497
BsoBI CYCGRG 1 cut(s) 1772
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 5 cut(s) 57, 226, 784, 1667, 1673
BspACI CCGC 4 cut(s) 16, 401, 780, 986
BspANI GGCC 6 cut(s) 15, 235, 768, 1328, 1513, 1631
BspCNI CTCAG 1 cut(s) 1083
BspLI GGNNCC 1 cut(s) 883
BspMAI CTGCAG 1 cut(s) 204
BspOI GCTAGC 1 cut(s) 423
BspPI GGATC 4 cut(s) 65, 234, 1662, 1668
BspTI CTTAAG 1 cut(s) 245
BspTNI GGTCTC 2 cut(s) 1242, 1497
BsrDI GCAATG 2 cut(s) 16, 655
BsrFI RCCGGY 1 cut(s) 132
BsrI ACTGG 3 cut(s) 699, 759, 1807
BssAI RCCGGY 1 cut(s) 132
BssECI CCNNGG 3 cut(s) 48, 1405, 1619
BssMI GATC 5 cut(s) 57, 226, 784, 1667, 1673
BssNI GRCGYC 2 cut(s) 1020, 1117
BssSI CACGAG 1 cut(s) 25
BssT1I CCWWGG 2 cut(s) 1405, 1619
Bst2BI CACGAG 1 cut(s) 25
Bst2UI CCWGG 3 cut(s) 50, 224, 318
Bst4CI ACNGT 5 cut(s) 310, 628, 743, 1255, 1285
Bst6I CTCTTC 2 cut(s) 718, 1500
BstACI GRCGYC 2 cut(s) 1020, 1117
BstAFI CTTAAG 1 cut(s) 245
BstAPI GCANNNNNTGC 2 cut(s) 512, 521
BstBAI YACGTR 2 cut(s) 557, 965
BstC8I GCNNGC 8 cut(s) 40, 173, 237, 421, 766, 778, 1018, 1041
BstDEI CTNAG 2 cut(s) 633, 1070
BstF5I GGATG 6 cut(s) 168, 703, 1196, 1438, 1811, 1815
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 5 cut(s) 60, 229, 787, 1670, 1676
BstMAI GTCTC 6 cut(s) 715, 732, 1242, 1286, 1497, 1775
BstMBI GATC 5 cut(s) 57, 226, 784, 1667, 1673
BstMWI GCNNNNNNNGC 7 cut(s) 15, 241, 512, 521, 852, 1049, 1637
BstNI CCWGG 3 cut(s) 50, 224, 318
BstNSI RCATGY 6 cut(s) 506, 528, 648, 978, 1043, 1194
BstSCI CCNGG 3 cut(s) 48, 222, 316
BstSFI CTRYAG 2 cut(s) 200, 1251
BstV1I GCAGC 5 cut(s) 839, 1023, 1039, 1158, 1489
BstX2I RGATCY 3 cut(s) 784, 1667, 1673
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 3 cut(s) 784, 1667, 1673
BsuI GTATCC 1 cut(s) 1374
BsuRI GGCC 6 cut(s) 15, 235, 768, 1328, 1513, 1631
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 6 cut(s) 168, 703, 1196, 1438, 1811, 1815
BtsI GCAGTG 2 cut(s) 192, 430
BtsIMutI CAGTG 6 cut(s) 192, 430, 692, 748, 752, 1078
Cac8I GCNNGC 8 cut(s) 40, 173, 237, 421, 766, 778, 1018, 1041
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 132
Cfr13I GGNCC 4 cut(s) 130, 220, 1481, 1511
CseI GACGC 3 cut(s) 1009, 1106, 1124
Csp6I GTAC 4 cut(s) 22, 482, 1256, 1800
CspCI CAANNNNNGTGG 2 cut(s) 1608, 1643
CviQI GTAC 4 cut(s) 22, 482, 1256, 1800
DdeI CTNAG 2 cut(s) 633, 1070
DpnI GATC 5 cut(s) 59, 228, 786, 1669, 1675
DpnII GATC 5 cut(s) 57, 226, 784, 1667, 1673
DriI GACNNNNNGTC 1 cut(s) 1101
EaeI YGGCCR 2 cut(s) 13, 1629
Eam1104I CTCTTC 2 cut(s) 718, 1500
Eam1105I GACNNNNNGTC 1 cut(s) 1101
EarI CTCTTC 2 cut(s) 718, 1500
Ecl136II GAGCTC 1 cut(s) 270
Eco130I CCWWGG 2 cut(s) 1405, 1619
Eco24I GRGCYC 1 cut(s) 272
Eco31I GGTCTC 2 cut(s) 1242, 1497
Eco47I GGWCC 3 cut(s) 130, 220, 1481
Eco53kI GAGCTC 1 cut(s) 270
Eco57I CTGAAG 2 cut(s) 894, 1442
Eco72I CACGTG 2 cut(s) 557, 965
Eco88I CYCGRG 1 cut(s) 1772
EcoICRI GAGCTC 1 cut(s) 270
EcoRII CCWGG 3 cut(s) 48, 222, 316
EcoT14I CCWWGG 2 cut(s) 1405, 1619
EcoT22I ATGCAT 2 cut(s) 526, 1718
EcoT38I GRGCYC 1 cut(s) 272
ErhI CCWWGG 2 cut(s) 1405, 1619
FaqI GGGAC 1 cut(s) 1600
FblI GTMKAC 2 cut(s) 72, 150
Fnu4HI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1503
FokI GGATG 4 cut(s) 175, 710, 1183, 1445
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1503
FspBI CTAG 1 cut(s) 420
FspI TGCGCA 1 cut(s) 176
GlaI GCGC 1 cut(s) 176
GluI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1503
HaeIII GGCC 6 cut(s) 15, 235, 768, 1328, 1513, 1631
HapII CCGG 1 cut(s) 133
HgaI GACGC 3 cut(s) 1009, 1106, 1124
HhaI GCGC 1 cut(s) 177
Hin1I GRCGYC 2 cut(s) 1020, 1117
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HincII GTYRAC 3 cut(s) 73, 151, 1650
HindII GTYRAC 3 cut(s) 73, 151, 1650
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 5 cut(s) 193, 205, 919, 1163, 1460
HpaII CCGG 1 cut(s) 133
Hpy166II GTNNAC 4 cut(s) 73, 151, 962, 1650
Hpy188I TCNGA 6 cut(s) 610, 874, 981, 1062, 1422, 1678
Hpy188III TCNNGA 6 cut(s) 571, 805, 1375, 1412, 1671, 1774
Hpy8I GTNNAC 4 cut(s) 73, 151, 962, 1650
Hpy99I CGWCG 4 cut(s) 74, 116, 155, 1140
HpyAV CCTTC 6 cut(s) 534, 779, 872, 1330, 1417, 1444
HpyCH4III ACNGT 5 cut(s) 310, 628, 743, 1255, 1285
HpyCH4IV ACGT 5 cut(s) 111, 556, 668, 964, 1706
HpyF10VI GCNNNNNNNGC 7 cut(s) 15, 241, 512, 521, 852, 1049, 1637
HpyF3I CTNAG 2 cut(s) 633, 1070
HpySE526I ACGT 5 cut(s) 111, 556, 668, 964, 1706
Hsp92I GRCGYC 2 cut(s) 1020, 1117
HspAI GCGC 1 cut(s) 175
Kzo9I GATC 5 cut(s) 57, 226, 784, 1667, 1673
LmnI GCTCC 1 cut(s) 1344
Lsp1109I GCAGC 5 cut(s) 839, 1023, 1039, 1158, 1489
LweI GCATC 7 cut(s) 511, 541, 989, 1003, 1183, 1703, 1725
MaeI CTAG 1 cut(s) 420
MaeII ACGT 5 cut(s) 111, 556, 668, 964, 1706
MaeIII GTNAC 6 cut(s) 259, 544, 793, 857, 945, 965
MalI GATC 5 cut(s) 59, 228, 786, 1669, 1675
MboI GATC 5 cut(s) 57, 226, 784, 1667, 1673
MboII GAAGA 8 cut(s) 115, 382, 705, 713, 887, 1487, 1761, 1791
MfeI CAATTG 3 cut(s) 516, 1110, 1575
MflI RGATCY 3 cut(s) 784, 1667, 1673
MhlI GDGCHC 1 cut(s) 272
MlsI TGGCCA 1 cut(s) 1631
MluNI TGGCCA 1 cut(s) 1631
MlyI GAGTC 2 cut(s) 928, 1157
MmeI TCCRAC 2 cut(s) 909, 1701
Mox20I TGGCCA 1 cut(s) 1631
Mph1103I ATGCAT 2 cut(s) 526, 1718
MroXI GAANNNNTTC 1 cut(s) 958
MscI TGGCCA 1 cut(s) 1631
MseI TTAA 5 cut(s) 246, 408, 831, 930, 1602
MslI CAYNNNNRTG 2 cut(s) 326, 359
Msp20I TGGCCA 1 cut(s) 1631
MspCI CTTAAG 1 cut(s) 245
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 3 cut(s) 50, 224, 318
MunI CAATTG 3 cut(s) 516, 1110, 1575
Mva1269I GAATGC 1 cut(s) 370
MvaI CCWGG 3 cut(s) 50, 224, 318
MwoI GCNNNNNNNGC 7 cut(s) 15, 241, 512, 521, 852, 1049, 1637
NdeII GATC 5 cut(s) 57, 226, 784, 1667, 1673
NheI GCTAGC 1 cut(s) 419
NlaIV GGNNCC 1 cut(s) 883
NmuCI GTSAC 3 cut(s) 259, 945, 965
NsbI TGCGCA 1 cut(s) 176
NsiI ATGCAT 2 cut(s) 526, 1718
NspI RCATGY 6 cut(s) 506, 528, 648, 978, 1043, 1194
PaeI GCATGC 1 cut(s) 1043
PciI ACATGT 1 cut(s) 1190
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 111
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 3 cut(s) 193, 205, 1460
PflMI CCANNNNNTGG 2 cut(s) 49, 1112
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 6 cut(s) 17, 854, 1038, 1054, 1173, 1504
PleI GAGTC 2 cut(s) 927, 1157
PmaCI CACGTG 2 cut(s) 557, 965
PmlI CACGTG 2 cut(s) 557, 965
PpsI GAGTC 2 cut(s) 927, 1157
Ppu21I YACGTR 2 cut(s) 557, 965
PscI ACATGT 1 cut(s) 1190
Psp124BI GAGCTC 1 cut(s) 272
Psp6I CCWGG 3 cut(s) 48, 222, 316
PspCI CACGTG 2 cut(s) 557, 965
PspGI CCWGG 3 cut(s) 48, 222, 316
PspN4I GGNNCC 1 cut(s) 883
PspPI GGNCC 4 cut(s) 130, 220, 1481, 1511
PstI CTGCAG 1 cut(s) 204
PstNI CAGNNNCTG 1 cut(s) 49
PsuI RGATCY 3 cut(s) 784, 1667, 1673
RsaI GTAC 4 cut(s) 23, 483, 1257, 1801
RsaNI GTAC 4 cut(s) 22, 482, 1256, 1800
RseI CAYNNNNRTG 2 cut(s) 326, 359
SacI GAGCTC 1 cut(s) 272
SalI GTCGAC 2 cut(s) 71, 149
SaqAI TTAA 5 cut(s) 246, 408, 831, 930, 1602
SatI GCNGC 6 cut(s) 16, 853, 1037, 1053, 1172, 1503
Sau3AI GATC 5 cut(s) 57, 226, 784, 1667, 1673
Sau96I GGNCC 4 cut(s) 130, 220, 1481, 1511
ScaI AGTACT 2 cut(s) 23, 483
SchI GAGTC 2 cut(s) 928, 1157
ScrFI CCNGG 3 cut(s) 50, 224, 318
SduI GDGCHC 1 cut(s) 272
SfaNI GCATC 7 cut(s) 511, 541, 989, 1003, 1183, 1703, 1725
SfcI CTRYAG 2 cut(s) 200, 1251
SinI GGWCC 3 cut(s) 130, 220, 1481
SmiMI CAYNNNNRTG 2 cut(s) 326, 359
SmlI CTYRAG 4 cut(s) 245, 803, 1412, 1785
SmoI CTYRAG 4 cut(s) 245, 803, 1412, 1785
SphI GCATGC 1 cut(s) 1043
SsiI CCGC 4 cut(s) 16, 401, 780, 986
SspI AATATT 2 cut(s) 578, 1382
SspMI CTAG 1 cut(s) 420
SstI GAGCTC 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 48, 222, 316
StyI CCWWGG 2 cut(s) 1405, 1619
TaaI ACNGT 5 cut(s) 310, 628, 743, 1255, 1285
TaiI ACGT 5 cut(s) 114, 559, 671, 967, 1709
TaqI TCGA 5 cut(s) 72, 150, 286, 1498, 1711
TatI WGTACW 4 cut(s) 21, 481, 1255, 1799
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 3 cut(s) 193, 205, 1460
Tru1I TTAA 5 cut(s) 246, 408, 831, 930, 1602
Tru9I TTAA 5 cut(s) 246, 408, 831, 930, 1602
TscAI CASTG 6 cut(s) 192, 430, 699, 748, 759, 1078
TseFI GTSAC 3 cut(s) 259, 945, 965
TseI GCWGC 5 cut(s) 852, 1036, 1052, 1171, 1502
Tsp45I GTSAC 3 cut(s) 259, 945, 965
TspGWI ACGGA 2 cut(s) 584, 1037
TspRI CASTG 6 cut(s) 192, 430, 699, 748, 759, 1078
Van91I CCANNNNNTGG 2 cut(s) 49, 1112
Vha464I CTTAAG 1 cut(s) 245
VpaK11BI GGWCC 3 cut(s) 130, 220, 1481
XapI RAATTY 2 cut(s) 250, 1315
XceI RCATGY 6 cut(s) 506, 528, 648, 978, 1043, 1194
XmiI GTMKAC 2 cut(s) 72, 150
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 1 cut(s) 420
ZrmI AGTACT 2 cut(s) 23, 483
Zsp2I ATGCAT 2 cut(s) 526, 1718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.