Rh5BG187500

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003776
Physical Location & Seq
Forward (+)
20577351 .. 20583705
6355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG187500.1

Sequence Viewer

Length: 1554 bp
ATGTGCAGCAATAGCAGCAACTACTCGTGCGAGACTGCCAGCCAGACCCTGGCATGGATCAACGCCATCGTCGACTTCATAAAACCCTTCTCTTTCTTGACCAACGCTCACGTCGTCAATTTCTTCAAGGATAGACTGTGGGAGGCGATTGATGAGGAATGGATGGATTGCTTACGGGAGGAAGCAGTCGAAAATCTGCTCCAAATTCCTTGCGGAGTGGTGCAGGATCACTGGCCGGCTTCGCTGAAGGAATTTATTGTTACTTTGAGGTCTCTTGCGCTTCCTCGACAGCAGGCCGACTTGCAGGCGGTATTGCCTGGAATGAGCATGACTTCACTCAACAGTGTTCTTTCGACGGGCATGAATTCGAAGAAGAAACATGAAGTAGAAGTTCTTTCTGCTGTTGTCAATTCAGTTGCAAACAGCATTGGTGCTCACGCAATAATTGATGTTGGTGCTGGTCAGGGGTATCTTGCACAAGTACTTTCCTTCCACTACCAACATTCGGTAATTGCAATTGATGCCTGCTCTCACCATGGAAGGGTTACCAATGCACGAGCAGAGCAAATCAAGAAACATTATGCTGCTCAGATGCGTAAATCTGTAGCAGGAAACAGGAGTTTGAATGTGCCTAAGGCAGTCACATGCAATGTGATGTCTATTGACATGTTGAAAGCTTTGGCTGATACATCACTGCATAAAGATAATGCCTCTCTACTACAATCTTCCTGCAATGCAGATTGTGAAACCTCATTGGTTCTTGCTGGGCTTCATGCTTGTGGAGATCTTTCGGTGACGATGCTCAAGACCTTTATGGAGTGCGAAGAGCTGGGCTTGTCACTTGGGAAAAGTTCGCGTGACCTTGCTTGTCAAAGTGCAGAGAGATGGAGTTGTCTGGAAAATGATGCTGGTCTCCATAATTTTGAGTTGCATACTTTCCGCGCTGCTTGTCAGATGGTTTTGAGTCTATATTATCCTGAAGTTGTGATAACCAGTCCATCCATTGGGCGTCAAGGGAAGGCTTTGCGTCGCCAGCAACGAAGGGGCATTCCAGACTCTGCGAATCCGAAAGAAAGCACATGTTCAACATTGCCTCAATTAGCTTGTAATATGAAAGAAAGTGGCTCTCACTTAAGAACCACAAAAATCGAAGAAGATGACAGTTTTGAGATGGAGTTCGAGACGACAGCCATAGGCAATGGACATTCATCTAGTAAGGCCACACAATGTGAGGGGACTCCACACATTAGCAAATATCCACTTTTTAGGAAATACTGTTTATCTGGATTATGTCACCTGGGTCTTGAGCCTCCGAAGGATACAGATCTCCATGGAATATGGAAGAAAGTTCAGCCTTTTGCTGAACTTATTGGACCTTATTGGACTCTTCGAGCTGCTTTCGGGCCTCTTTTGGAAACTTTACTTTTGCTTGATAGATTATTGTTCCTACAAGAGCAAGGCAGTTCAATGGAAGTAGTCATGTTACCTATTTTTGATCCAGCATTATCCCCAAGGAATGTGGCCATTATTGCTAAAAAGTTGACACAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_32

Protein Analysis

517

Amino Acids

56.95

Weight (kDa)

6.23

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_32 PF13679 124 - 273 7.3e-20 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 49, 1004
AccI GTMKAC 1 cut(s) 72
AccII CGCG 2 cut(s) 856, 942
AciI CCGC 3 cut(s) 213, 308, 940
AclWI GGATC 3 cut(s) 65, 234, 1490
AcoI YGGCCR 2 cut(s) 233, 1521
AcsI RAATTY 4 cut(s) 204, 251, 364, 1548
AcuI CTGAAG 2 cut(s) 266, 999
AcyI GRCGYC 1 cut(s) 1009
AdeI CACNNNGTG 1 cut(s) 1229
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1004
AflII CTTAAG 1 cut(s) 1132
AflIII ACRYGT 2 cut(s) 666, 1079
AgsI TTSAA 5 cut(s) 127, 625, 673, 1086, 1467
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 3 cut(s) 48, 316, 1296
AluBI AGCT 4 cut(s) 677, 829, 1103, 1394
AluI AGCT 4 cut(s) 677, 829, 1103, 1394
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 4 cut(s) 26, 276, 917, 1175
AlwI GGATC 3 cut(s) 65, 234, 1490
AlwNI CAGNNNCTG 2 cut(s) 49, 1058
AoxI GGCC 5 cut(s) 233, 294, 1218, 1403, 1521
ApeKI GCWGC 5 cut(s) 6, 15, 584, 944, 1394
ApoI RAATTY 4 cut(s) 204, 251, 364, 1548
AspLEI GCGC 2 cut(s) 280, 944
AspS9I GGNCC 2 cut(s) 1373, 1403
AsuHPI GGTGA 3 cut(s) 524, 805, 1286
AsuII TTCGAA 1 cut(s) 368
AvaII GGWCC 1 cut(s) 1373
AxyI CCTNAGG 1 cut(s) 633
BalI TGGCCA 1 cut(s) 1523
BauI CACGAG 2 cut(s) 25, 555
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 5 cut(s) 18, 27, 571, 931, 1381
BccI CCATC 6 cut(s) 74, 157, 879, 949, 1006, 1165
BciT130I CCWGG 3 cut(s) 50, 318, 1298
BciVI GTATCC 1 cut(s) 1312
BcoDI GTCTC 4 cut(s) 26, 276, 917, 1175
BfaI CTAG 1 cut(s) 1212
BfmI CTRYAG 1 cut(s) 603
BfrI CTTAAG 1 cut(s) 1132
BfuI GTATCC 1 cut(s) 1312
BglII AGATCT 2 cut(s) 784, 1324
BisI GCNGC 5 cut(s) 7, 16, 585, 945, 1395
BlsI GCNGC 5 cut(s) 8, 17, 586, 946, 1396
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 3 cut(s) 50, 318, 1298
Bme18I GGWCC 1 cut(s) 1373
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 1373, 1403
BmrFI CCNGG 3 cut(s) 50, 318, 1298
BmsI GCATC 4 cut(s) 511, 582, 789, 895
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 2 cut(s) 788, 1325
BsaBI GATNNNNATC 1 cut(s) 1323
BsaHI GRCGYC 1 cut(s) 1009
BsaI GGTCTC 2 cut(s) 276, 917
BsaJI CCNNGG 5 cut(s) 48, 535, 1297, 1330, 1511
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1004
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 236, 993
Bse21I CCTNAGG 1 cut(s) 633
Bse3DI GCAATG 4 cut(s) 655, 739, 1088, 1204
Bse8I GATNNNNATC 1 cut(s) 1323
BseBI CCWGG 3 cut(s) 50, 318, 1298
BseDI CCNNGG 5 cut(s) 48, 535, 1297, 1330, 1511
BseGI GGATG 2 cut(s) 168, 998
BseJI GATNNNNATC 1 cut(s) 1323
BseLI CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1004
BseMI GCAATG 4 cut(s) 655, 739, 1088, 1204
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 2 cut(s) 236, 993
BseXI GCAGC 5 cut(s) 18, 27, 571, 931, 1381
BseYI CCCAGC 2 cut(s) 764, 829
BsgI GTGCAG 3 cut(s) 25, 242, 897
Bsh1236I CGCG 2 cut(s) 856, 942
BshFI GGCC 5 cut(s) 235, 296, 1220, 1405, 1523
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 1249
BslI CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1004
BsmAI GTCTC 4 cut(s) 26, 276, 917, 1175
BsmBI CGTCTC 1 cut(s) 1175
BsmFI GGGAC 1 cut(s) 1249
BsmI GAATGC 1 cut(s) 1047
BsnI GGCC 5 cut(s) 235, 296, 1220, 1405, 1523
Bso31I GGTCTC 2 cut(s) 276, 917
Bsp119I TTCGAA 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 5 cut(s) 57, 226, 784, 1324, 1495
Bsp19I CCATGG 2 cut(s) 535, 1330
BspACI CCGC 3 cut(s) 213, 308, 940
BspANI GGCC 5 cut(s) 235, 296, 1220, 1405, 1523
BspCNI CTCAG 1 cut(s) 601
BspFNI CGCG 2 cut(s) 856, 942
BspPI GGATC 3 cut(s) 65, 234, 1490
BspQI GCTCTTC 1 cut(s) 819
BspT104I TTCGAA 1 cut(s) 368
BspTI CTTAAG 1 cut(s) 1132
BspTNI GGTCTC 2 cut(s) 276, 917
BsrDI GCAATG 4 cut(s) 655, 739, 1088, 1204
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 2 cut(s) 236, 993
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 5 cut(s) 48, 535, 1297, 1330, 1511
BssMI GATC 5 cut(s) 57, 226, 784, 1324, 1495
BssNI GRCGYC 1 cut(s) 1009
BssSI CACGAG 2 cut(s) 25, 555
BssT1I CCWWGG 3 cut(s) 535, 1330, 1511
Bst2BI CACGAG 2 cut(s) 25, 555
Bst2UI CCWGG 3 cut(s) 50, 318, 1298
Bst4CI ACNGT 4 cut(s) 138, 344, 1163, 1277
Bst6I CTCTTC 2 cut(s) 819, 1392
BstACI GRCGYC 1 cut(s) 1009
BstAFI CTTAAG 1 cut(s) 1132
BstAPI GCANNNNNTGC 1 cut(s) 521
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 6 cut(s) 40, 237, 294, 306, 526, 1034
BstDEI CTNAG 2 cut(s) 588, 633
BstDSI CCRYGG 2 cut(s) 535, 1330
BstEII GGTNACC 1 cut(s) 544
BstF5I GGATG 2 cut(s) 168, 998
BstFNI CGCG 2 cut(s) 856, 942
BstHHI GCGC 2 cut(s) 280, 944
BstKTI GATC 5 cut(s) 60, 229, 787, 1327, 1498
BstMAI GTCTC 4 cut(s) 26, 276, 917, 1175
BstMBI GATC 5 cut(s) 57, 226, 784, 1324, 1495
BstMWI GCNNNNNNNGC 6 cut(s) 12, 15, 241, 521, 1033, 1529
BstNI CCWGG 3 cut(s) 50, 318, 1298
BstNSI RCATGY 3 cut(s) 648, 670, 1083
BstPI GGTNACC 1 cut(s) 544
BstSCI CCNGG 3 cut(s) 48, 316, 1296
BstSFI CTRYAG 1 cut(s) 603
BstUI CGCG 2 cut(s) 856, 942
BstV1I GCAGC 5 cut(s) 18, 27, 571, 931, 1381
BstX2I RGATCY 2 cut(s) 784, 1324
BstYI RGATCY 2 cut(s) 784, 1324
Bsu36I CCTNAGG 1 cut(s) 633
BsuI GTATCC 1 cut(s) 1312
BsuRI GGCC 5 cut(s) 235, 296, 1220, 1405, 1523
BtgI CCRYGG 2 cut(s) 535, 1330
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 2 cut(s) 168, 998
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 3 cut(s) 229, 349, 692
Cac8I GCNNGC 6 cut(s) 40, 237, 294, 306, 526, 1034
CaiI CAGNNNCTG 2 cut(s) 49, 1058
CfoI GCGC 2 cut(s) 280, 944
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 1373, 1403
CseI GACGC 2 cut(s) 998, 1016
Csp6I GTAC 1 cut(s) 482
CspCI CAANNNNNGTGG 2 cut(s) 1500, 1535
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 2 cut(s) 588, 633
DpnI GATC 5 cut(s) 59, 228, 786, 1326, 1497
DpnII GATC 5 cut(s) 57, 226, 784, 1324, 1495
DraIII CACNNNGTG 1 cut(s) 1229
EaeI YGGCCR 2 cut(s) 233, 1521
Eam1104I CTCTTC 2 cut(s) 819, 1392
EarI CTCTTC 2 cut(s) 819, 1392
Eco130I CCWWGG 3 cut(s) 535, 1330, 1511
Eco31I GGTCTC 2 cut(s) 276, 917
Eco47I GGWCC 1 cut(s) 1373
Eco57I CTGAAG 2 cut(s) 266, 999
Eco81I CCTNAGG 1 cut(s) 633
Eco91I GGTNACC 1 cut(s) 544
EcoO65I GGTNACC 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 3 cut(s) 48, 316, 1296
EcoT14I CCWWGG 3 cut(s) 535, 1330, 1511
ErhI CCWWGG 3 cut(s) 535, 1330, 1511
Esp3I CGTCTC 1 cut(s) 1175
FaqI GGGAC 1 cut(s) 1249
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 7, 16, 585, 945, 1395
FokI GGATG 2 cut(s) 175, 985
Fsp4HI GCNGC 5 cut(s) 7, 16, 585, 945, 1395
FspBI CTAG 1 cut(s) 1212
GlaI GCGC 2 cut(s) 279, 943
GluI GCNGC 5 cut(s) 7, 16, 585, 945, 1395
GsaI CCCAGC 2 cut(s) 768, 833
HaeIII GGCC 5 cut(s) 235, 296, 1220, 1405, 1523
HapII CCGG 1 cut(s) 236
HgaI GACGC 2 cut(s) 998, 1016
HhaI GCGC 2 cut(s) 280, 944
Hin1I GRCGYC 1 cut(s) 1009
Hin6I GCGC 2 cut(s) 278, 942
HinP1I GCGC 2 cut(s) 278, 942
HincII GTYRAC 2 cut(s) 73, 1542
HindII GTYRAC 2 cut(s) 73, 1542
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 5 cut(s) 964, 1055, 1063, 1237, 1384
HpaII CCGG 1 cut(s) 236
HphI GGTGA 3 cut(s) 524, 805, 1286
Hpy166II GTNNAC 2 cut(s) 73, 1542
Hpy188I TCNGA 4 cut(s) 591, 954, 1068, 1314
Hpy188III TCNNGA 9 cut(s) 97, 571, 805, 896, 977, 1052, 1180, 1284, 1304
Hpy8I GTNNAC 2 cut(s) 73, 1542
Hpy99I CGWCG 4 cut(s) 74, 116, 358, 1032
HpyAV CCTTC 7 cut(s) 97, 241, 499, 534, 1012, 1035, 1309
HpyCH4III ACNGT 4 cut(s) 138, 344, 1163, 1277
HpyCH4IV ACGT 1 cut(s) 111
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 15, 241, 521, 1033, 1529
HpyF3I CTNAG 2 cut(s) 588, 633
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 1 cut(s) 1009
HspAI GCGC 2 cut(s) 278, 942
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Kzo9I GATC 5 cut(s) 57, 226, 784, 1324, 1495
LguI GCTCTTC 1 cut(s) 819
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 5 cut(s) 18, 27, 571, 931, 1381
LweI GCATC 4 cut(s) 511, 582, 789, 895
MaeI CTAG 1 cut(s) 1212
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 8 cut(s) 259, 544, 640, 793, 837, 857, 1292, 1482
MalI GATC 5 cut(s) 59, 228, 786, 1326, 1497
MboI GATC 5 cut(s) 57, 226, 784, 1324, 1495
MboII GAAGA 9 cut(s) 115, 382, 385, 717, 836, 1163, 1166, 1354, 1379
MfeI CAATTG 1 cut(s) 516
MflI RGATCY 2 cut(s) 784, 1324
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 1523
MluNI TGGCCA 1 cut(s) 1523
MlyI GAGTC 4 cut(s) 973, 1049, 1231, 1378
Mox20I TGGCCA 1 cut(s) 1523
MroNI GCCGGC 1 cut(s) 235
MscI TGGCCA 1 cut(s) 1523
MseI TTAA 1 cut(s) 1133
MslI CAYNNNNRTG 1 cut(s) 777
Msp20I TGGCCA 1 cut(s) 1523
MspCI CTTAAG 1 cut(s) 1132
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 3 cut(s) 50, 318, 1298
MunI CAATTG 1 cut(s) 516
Mva1269I GAATGC 1 cut(s) 1047
MvaI CCWGG 3 cut(s) 50, 318, 1298
MvnI CGCG 2 cut(s) 856, 942
MwoI GCNNNNNNNGC 6 cut(s) 12, 15, 241, 521, 1033, 1529
NaeI GCCGGC 1 cut(s) 237
NcoI CCATGG 2 cut(s) 535, 1330
NdeII GATC 5 cut(s) 57, 226, 784, 1324, 1495
NgoMIV GCCGGC 1 cut(s) 235
NmuCI GTSAC 5 cut(s) 640, 793, 837, 857, 1292
NspI RCATGY 3 cut(s) 648, 670, 1083
NspV TTCGAA 1 cut(s) 368
PciI ACATGT 2 cut(s) 666, 1079
PciSI GCTCTTC 1 cut(s) 819
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 111, 1036
PctI GAATGC 1 cut(s) 1047
PdiI GCCGGC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 1063
PflMI CCANNNNNTGG 2 cut(s) 49, 1004
PkrI GCNGC 5 cut(s) 8, 17, 586, 946, 1396
PleI GAGTC 4 cut(s) 972, 1049, 1231, 1378
PpsI GAGTC 4 cut(s) 972, 1049, 1231, 1378
PscI ACATGT 2 cut(s) 666, 1079
Psp6I CCWGG 3 cut(s) 48, 316, 1296
PspEI GGTNACC 1 cut(s) 544
PspFI CCCAGC 2 cut(s) 764, 829
PspGI CCWGG 3 cut(s) 48, 316, 1296
PspPI GGNCC 2 cut(s) 1373, 1403
PstNI CAGNNNCTG 2 cut(s) 49, 1058
PsuI RGATCY 2 cut(s) 784, 1324
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 777
SalI GTCGAC 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 819
SaqAI TTAA 1 cut(s) 1133
SatI GCNGC 5 cut(s) 7, 16, 585, 945, 1395
Sau3AI GATC 5 cut(s) 57, 226, 784, 1324, 1495
Sau96I GGNCC 2 cut(s) 1373, 1403
ScaI AGTACT 1 cut(s) 483
SchI GAGTC 4 cut(s) 973, 1049, 1231, 1378
ScrFI CCNGG 3 cut(s) 50, 318, 1298
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 4 cut(s) 511, 582, 789, 895
SfcI CTRYAG 1 cut(s) 603
SfuI TTCGAA 1 cut(s) 368
SinI GGWCC 1 cut(s) 1373
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 3 cut(s) 803, 1132, 1304
SmoI CTYRAG 3 cut(s) 803, 1132, 1304
SsiI CCGC 3 cut(s) 213, 308, 940
SspMI CTAG 1 cut(s) 1212
StyD4I CCNGG 3 cut(s) 48, 316, 1296
StyI CCWWGG 3 cut(s) 535, 1330, 1511
TaaI ACNGT 4 cut(s) 138, 344, 1163, 1277
TaiI ACGT 1 cut(s) 114
TaqI TCGA 8 cut(s) 72, 189, 286, 353, 368, 1149, 1179, 1390
TatI WGTACW 1 cut(s) 481
TfiI GAWTC 1 cut(s) 1063
Tru1I TTAA 1 cut(s) 1133
Tru9I TTAA 1 cut(s) 1133
TscAI CASTG 3 cut(s) 236, 349, 699
TseFI GTSAC 5 cut(s) 640, 793, 837, 857, 1292
TseI GCWGC 5 cut(s) 6, 15, 584, 944, 1394
Tsp45I GTSAC 5 cut(s) 640, 793, 837, 857, 1292
TspDTI ATGAA 6 cut(s) 67, 377, 396, 761, 1127, 1197
TspRI CASTG 3 cut(s) 236, 349, 699
Van91I CCANNNNNTGG 2 cut(s) 49, 1004
Vha464I CTTAAG 1 cut(s) 1132
VpaK11BI GGWCC 1 cut(s) 1373
XapI RAATTY 4 cut(s) 204, 251, 364, 1548
XceI RCATGY 3 cut(s) 648, 670, 1083
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 1 cut(s) 1212
ZrmI AGTACT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.