RLG00000032976

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003776
Physical Location & Seq
Forward (+)
21611768 .. 21617986
6219 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032976

Sequence Viewer

Length: 1662 bp
ATGTGCAGCAATAGCAGCAACTACTCGTGCGAGACTGCCAGCCAGACCCTGGCATGGATCAACGCCATCATCGACTTCATTAAACCCTTCTCTTTCTTGACCAACGCTCACGTCGTCAATTTCTTCAAGGATAGGCTGTGGGAGGCGATTGATGAGGAATGGATGGATTGCTTACGGGAGGAAGCAGTCGAAAATCTGCTCCAAATTCCTGGCGGAGTGGTGCAGGATCACTGGCCGGCTTCGCTGAAGGAATTTATTGTTACTTTGAGGTCTCTTGCGCTTCCTCGACAGCAGGCCGACTTGCAGGCGGTATTGCCTGGAATGAGCATGACCTCACTCAACAGTGTTCTTTCGACGGGCATGAATTCGAAGAAGAAACATGAAGTAGAATTTCTTTCTGCTGTTGTCAATTCAGTTGCAAACAGCATCGGTGCTCACGCAATAATTGACGTTGGTGCTGGTCAGGGGTATCTTGCACAAGTACTTTCCTTCCACTACCAACATTCGGTAATTGCAATTGATGCCTGCTCTCACCATGGAAGGGTTACCAATGCACGTGCAGAGCAAATCAAGAAACATTATGCTGCTCAGATGCGTAAATCTGTAGCAGGAAACAGGAGTTTGAATGTGCCTAAGACAGTCACATGCAATGTGATGTCTATTGACATGTTGAAAGCTTTGGCTGATACATCACTGCATAAAGATAATGCCTCTCTACTACAATCTTCCTGCAATGCAGATTGTGAAACATCATTGGTTCTTGCTGGGCTTCATGCTTGTGGAGATCTTTCGGTGACGATGCTCAAGACCTTTATGGAGTGCGAAGAGGTTAAAGCAGTTGTTAGTGTTGGCTGCTGTTACAACCTATTGTCTGAAGAGGGTTTCAACCATGTGGGGTCTCAATGTGGTTTTCCCATGAGTTGTGGTGTCAAAGCTGCTGGCTTGTCACTTGGAAAAAGTTCACGAGACCTTGCTTGTCAAAGTGCAGAGAGATGGAGTTGTCTGGAAAATGATGCTGGTATCCATAATTTTGAGTTGCATACTTTCCGTGCTGCTTTTCAGATGGTTTTGAGTCTATATTATCCTGAAGTTGTGATAACCAGTCCATCCATTGGGCGTCAAGGGAAGGCTTTGCGTCGCCAGCAACGAAGGGGCATTCCAGACTCTGCGAATCCGAAAGAAAGCACATGTTCAACATTGCCTCAATTAGCTTGTAATATGAAAGGAAGTGGCTCTCACCTAAAAACCACAAAAATCGAAGAAGATGACAGTTTTGAGATGGAGTTCGAGACGACAGCCATAGGCAAAGGACATTCATCTAGTAAGGCCACACAATGTGAGGGGACTCCACACATTAGCAAATATCCACTTTTTAAGAAATACTGTTTGTCTGGATTATGTCGCCTGGGTCTTGAGCCTCCGAAGGATACAGATCTCCATGGAATATGGAAGAAAGTTCAGCCTTTTACTGAACTTATTGGACCTTATTGGACTCTTCGAGCTGCTTTTGGGCCTCTTTTGGAAACTTTACTTTTGCTTGATAGATTATTGTTCCTACAAGAGCAAGGCAGTTCAATTGAAGTAGTCATGTTACCTATTTTTGATCCAGCATTATCCCCAAGGAATGTGGCCATTATTGCTAAAAAGTTGACACAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_32

Protein Analysis

554

Amino Acids

60.62

Weight (kDa)

6.53

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_32 PF13679 124 - 292 5.3e-27 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 49, 1112
AciI CCGC 2 cut(s) 213, 308
AclWI GGATC 3 cut(s) 65, 234, 1598
AcoI YGGCCR 2 cut(s) 233, 1629
AcsI RAATTY 5 cut(s) 204, 251, 364, 389, 1656
AcuI CTGAAG 3 cut(s) 266, 894, 1107
AcvI CACGTG 1 cut(s) 557
AcyI GRCGYC 1 cut(s) 1117
AdeI CACNNNGTG 1 cut(s) 1337
AfaI GTAC 1 cut(s) 483
AfiI CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1112
AflIII ACRYGT 2 cut(s) 666, 1187
AgsI TTSAA 7 cut(s) 127, 625, 673, 886, 1194, 1575, 1580
AjiI CACGTC 1 cut(s) 112
AjnI CCWGG 4 cut(s) 48, 208, 316, 1404
AluBI AGCT 4 cut(s) 677, 935, 1211, 1502
AluI AGCT 4 cut(s) 677, 935, 1211, 1502
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 5 cut(s) 26, 276, 903, 960, 1283
AlwI GGATC 3 cut(s) 65, 234, 1598
AlwNI CAGNNNCTG 2 cut(s) 49, 1166
AoxI GGCC 5 cut(s) 233, 294, 1326, 1511, 1629
ApeKI GCWGC 7 cut(s) 6, 15, 584, 852, 935, 1052, 1502
ApoI RAATTY 5 cut(s) 204, 251, 364, 389, 1656
Asp700I GAANNNNTTC 1 cut(s) 958
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 2 cut(s) 1481, 1511
AsuHPI GGTGA 3 cut(s) 524, 805, 1229
AsuII TTCGAA 1 cut(s) 368
AvaII GGWCC 1 cut(s) 1481
BalI TGGCCA 1 cut(s) 1631
BauI CACGAG 2 cut(s) 25, 963
BbrPI CACGTG 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 7 cut(s) 18, 27, 571, 839, 922, 1039, 1489
BccI CCATC 6 cut(s) 74, 157, 987, 1057, 1114, 1273
BciT130I CCWGG 4 cut(s) 50, 210, 318, 1406
BciVI GTATCC 2 cut(s) 1031, 1420
BcoDI GTCTC 5 cut(s) 26, 276, 903, 960, 1283
BfaI CTAG 1 cut(s) 1320
BfmI CTRYAG 1 cut(s) 603
BfuI GTATCC 2 cut(s) 1031, 1420
BglII AGATCT 2 cut(s) 784, 1432
BisI GCNGC 7 cut(s) 7, 16, 585, 853, 936, 1053, 1503
BlsI GCNGC 7 cut(s) 8, 17, 586, 854, 937, 1054, 1504
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 4 cut(s) 50, 210, 318, 1406
Bme18I GGWCC 1 cut(s) 1481
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 1481, 1511
BmrFI CCNGG 4 cut(s) 50, 210, 318, 1406
BmsI GCATC 5 cut(s) 435, 511, 582, 789, 1003
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 2 cut(s) 788, 1433
BsaAI YACGTR 1 cut(s) 557
BsaBI GATNNNNATC 1 cut(s) 1431
BsaHI GRCGYC 1 cut(s) 1117
BsaI GGTCTC 3 cut(s) 276, 903, 960
BsaJI CCNNGG 5 cut(s) 48, 535, 1405, 1438, 1619
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1112
Bse118I RCCGGY 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 236, 1101
Bse3DI GCAATG 3 cut(s) 655, 739, 1196
Bse8I GATNNNNATC 1 cut(s) 1431
BseBI CCWGG 4 cut(s) 50, 210, 318, 1406
BseDI CCNNGG 5 cut(s) 48, 535, 1405, 1438, 1619
BseGI GGATG 2 cut(s) 168, 1106
BseJI GATNNNNATC 1 cut(s) 1431
BseLI CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1112
BseMI GCAATG 3 cut(s) 655, 739, 1196
BseMII CTCAG 1 cut(s) 602
BseNI ACTGG 2 cut(s) 236, 1101
BseXI GCAGC 7 cut(s) 18, 27, 571, 839, 922, 1039, 1489
BseYI CCCAGC 1 cut(s) 764
BsgI GTGCAG 4 cut(s) 25, 242, 579, 1005
BshFI GGCC 5 cut(s) 235, 296, 1328, 1513, 1631
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 1357
BslI CCNNNNNNNGG 5 cut(s) 49, 54, 505, 541, 1112
BsmAI GTCTC 5 cut(s) 26, 276, 903, 960, 1283
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 1 cut(s) 1357
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 5 cut(s) 235, 296, 1328, 1513, 1631
Bso31I GGTCTC 3 cut(s) 276, 903, 960
Bsp119I TTCGAA 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 5 cut(s) 57, 226, 784, 1432, 1603
Bsp19I CCATGG 2 cut(s) 535, 1438
BspACI CCGC 2 cut(s) 213, 308
BspANI GGCC 5 cut(s) 235, 296, 1328, 1513, 1631
BspCNI CTCAG 1 cut(s) 601
BspPI GGATC 3 cut(s) 65, 234, 1598
BspT104I TTCGAA 1 cut(s) 368
BspTNI GGTCTC 3 cut(s) 276, 903, 960
BsrDI GCAATG 3 cut(s) 655, 739, 1196
BsrFI RCCGGY 1 cut(s) 235
BsrI ACTGG 2 cut(s) 236, 1101
BssAI RCCGGY 1 cut(s) 235
BssECI CCNNGG 5 cut(s) 48, 535, 1405, 1438, 1619
BssMI GATC 5 cut(s) 57, 226, 784, 1432, 1603
BssNI GRCGYC 1 cut(s) 1117
BssSI CACGAG 2 cut(s) 25, 963
BssT1I CCWWGG 3 cut(s) 535, 1438, 1619
Bst2BI CACGAG 2 cut(s) 25, 963
Bst2UI CCWGG 4 cut(s) 50, 210, 318, 1406
Bst4CI ACNGT 4 cut(s) 344, 640, 1271, 1385
Bst6I CTCTTC 3 cut(s) 819, 870, 1500
BstACI GRCGYC 1 cut(s) 1117
BstAPI GCANNNNNTGC 1 cut(s) 521
BstBAI YACGTR 1 cut(s) 557
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 7 cut(s) 40, 237, 294, 306, 526, 940, 1142
BstDEI CTNAG 2 cut(s) 588, 633
BstDSI CCRYGG 2 cut(s) 535, 1438
BstEII GGTNACC 1 cut(s) 544
BstF5I GGATG 2 cut(s) 168, 1106
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 5 cut(s) 60, 229, 787, 1435, 1606
BstMAI GTCTC 5 cut(s) 26, 276, 903, 960, 1283
BstMBI GATC 5 cut(s) 57, 226, 784, 1432, 1603
BstMWI GCNNNNNNNGC 6 cut(s) 12, 15, 241, 521, 1141, 1637
BstNI CCWGG 4 cut(s) 50, 210, 318, 1406
BstNSI RCATGY 3 cut(s) 648, 670, 1191
BstPI GGTNACC 1 cut(s) 544
BstSCI CCNGG 4 cut(s) 48, 208, 316, 1404
BstSFI CTRYAG 1 cut(s) 603
BstV1I GCAGC 7 cut(s) 18, 27, 571, 839, 922, 1039, 1489
BstX2I RGATCY 2 cut(s) 784, 1432
BstXI CCANNNNNNTGG 1 cut(s) 209
BstYI RGATCY 2 cut(s) 784, 1432
BsuI GTATCC 2 cut(s) 1031, 1420
BsuRI GGCC 5 cut(s) 235, 296, 1328, 1513, 1631
BtgI CCRYGG 2 cut(s) 535, 1438
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 2 cut(s) 168, 1106
BtsI GCAGTG 1 cut(s) 692
BtsIMutI CAGTG 3 cut(s) 229, 349, 692
Cac8I GCNNGC 7 cut(s) 40, 237, 294, 306, 526, 940, 1142
CaiI CAGNNNCTG 2 cut(s) 49, 1166
CfoI GCGC 1 cut(s) 280
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 2 cut(s) 1481, 1511
CseI GACGC 2 cut(s) 1106, 1124
Csp6I GTAC 1 cut(s) 482
CspCI CAANNNNNGTGG 2 cut(s) 1608, 1643
CviQI GTAC 1 cut(s) 482
DdeI CTNAG 2 cut(s) 588, 633
DpnI GATC 5 cut(s) 59, 228, 786, 1434, 1605
DpnII GATC 5 cut(s) 57, 226, 784, 1432, 1603
DraIII CACNNNGTG 1 cut(s) 1337
EaeI YGGCCR 2 cut(s) 233, 1629
Eam1104I CTCTTC 3 cut(s) 819, 870, 1500
EarI CTCTTC 3 cut(s) 819, 870, 1500
EciI GGCGGA 1 cut(s) 228
Eco130I CCWWGG 3 cut(s) 535, 1438, 1619
Eco31I GGTCTC 3 cut(s) 276, 903, 960
Eco47I GGWCC 1 cut(s) 1481
Eco57I CTGAAG 3 cut(s) 266, 894, 1107
Eco72I CACGTG 1 cut(s) 557
Eco91I GGTNACC 1 cut(s) 544
EcoO65I GGTNACC 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 4 cut(s) 48, 208, 316, 1404
EcoT14I CCWWGG 3 cut(s) 535, 1438, 1619
ErhI CCWWGG 3 cut(s) 535, 1438, 1619
Esp3I CGTCTC 1 cut(s) 1283
FaqI GGGAC 1 cut(s) 1357
Fnu4HI GCNGC 7 cut(s) 7, 16, 585, 853, 936, 1053, 1503
FokI GGATG 2 cut(s) 175, 1093
Fsp4HI GCNGC 7 cut(s) 7, 16, 585, 853, 936, 1053, 1503
FspBI CTAG 1 cut(s) 1320
GlaI GCGC 1 cut(s) 279
GluI GCNGC 7 cut(s) 7, 16, 585, 853, 936, 1053, 1503
GsaI CCCAGC 1 cut(s) 768
HaeIII GGCC 5 cut(s) 235, 296, 1328, 1513, 1631
HapII CCGG 1 cut(s) 236
HgaI GACGC 2 cut(s) 1106, 1124
HhaI GCGC 1 cut(s) 280
Hin1I GRCGYC 1 cut(s) 1117
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 1 cut(s) 1650
HindII GTYRAC 1 cut(s) 1650
HindIII AAGCTT 1 cut(s) 675
HinfI GANTC 5 cut(s) 1072, 1163, 1171, 1345, 1492
HpaII CCGG 1 cut(s) 236
HphI GGTGA 3 cut(s) 524, 805, 1229
Hpy166II GTNNAC 2 cut(s) 962, 1650
Hpy188I TCNGA 5 cut(s) 591, 874, 1062, 1176, 1422
Hpy8I GTNNAC 2 cut(s) 962, 1650
Hpy99I CGWCG 3 cut(s) 116, 358, 1140
HpyAV CCTTC 7 cut(s) 97, 241, 499, 534, 1120, 1143, 1417
HpyCH4III ACNGT 4 cut(s) 344, 640, 1271, 1385
HpyCH4IV ACGT 3 cut(s) 111, 450, 556
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 15, 241, 521, 1141, 1637
HpyF3I CTNAG 2 cut(s) 588, 633
HpySE526I ACGT 3 cut(s) 111, 450, 556
Hsp92I GRCGYC 1 cut(s) 1117
HspAI GCGC 1 cut(s) 278
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Kzo9I GATC 5 cut(s) 57, 226, 784, 1432, 1603
LmnI GCTCC 1 cut(s) 204
Lsp1109I GCAGC 7 cut(s) 18, 27, 571, 839, 922, 1039, 1489
LweI GCATC 5 cut(s) 435, 511, 582, 789, 1003
MaeI CTAG 1 cut(s) 1320
MaeII ACGT 3 cut(s) 111, 450, 556
MaeIII GTNAC 7 cut(s) 259, 544, 640, 793, 857, 945, 1590
MalI GATC 5 cut(s) 59, 228, 786, 1434, 1605
MboI GATC 5 cut(s) 57, 226, 784, 1432, 1603
MfeI CAATTG 2 cut(s) 516, 1575
MflI RGATCY 2 cut(s) 784, 1432
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 1631
MluNI TGGCCA 1 cut(s) 1631
MlyI GAGTC 4 cut(s) 1081, 1157, 1339, 1486
Mox20I TGGCCA 1 cut(s) 1631
MroNI GCCGGC 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 958
MscI TGGCCA 1 cut(s) 1631
MseI TTAA 3 cut(s) 81, 831, 1374
MslI CAYNNNNRTG 1 cut(s) 777
Msp20I TGGCCA 1 cut(s) 1631
MspI CCGG 1 cut(s) 236
MspR9I CCNGG 4 cut(s) 50, 210, 318, 1406
MunI CAATTG 2 cut(s) 516, 1575
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 4 cut(s) 50, 210, 318, 1406
MwoI GCNNNNNNNGC 6 cut(s) 12, 15, 241, 521, 1141, 1637
NaeI GCCGGC 1 cut(s) 237
NcoI CCATGG 2 cut(s) 535, 1438
NdeII GATC 5 cut(s) 57, 226, 784, 1432, 1603
NgoMIV GCCGGC 1 cut(s) 235
NmuCI GTSAC 3 cut(s) 640, 793, 945
NspI RCATGY 3 cut(s) 648, 670, 1191
NspV TTCGAA 1 cut(s) 368
PciI ACATGT 2 cut(s) 666, 1187
PcsI WCGNNNNNNNCGW 3 cut(s) 69, 111, 1144
PctI GAATGC 1 cut(s) 1155
PdiI GCCGGC 1 cut(s) 237
PdmI GAANNNNTTC 1 cut(s) 958
PfeI GAWTC 1 cut(s) 1171
PflMI CCANNNNNTGG 2 cut(s) 49, 1112
PkrI GCNGC 7 cut(s) 8, 17, 586, 854, 937, 1054, 1504
PleI GAGTC 4 cut(s) 1080, 1157, 1339, 1486
PmaCI CACGTG 1 cut(s) 557
PmlI CACGTG 1 cut(s) 557
PpsI GAGTC 4 cut(s) 1080, 1157, 1339, 1486
Ppu21I YACGTR 1 cut(s) 557
PscI ACATGT 2 cut(s) 666, 1187
Psp6I CCWGG 4 cut(s) 48, 208, 316, 1404
PspCI CACGTG 1 cut(s) 557
PspEI GGTNACC 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 764
PspGI CCWGG 4 cut(s) 48, 208, 316, 1404
PspPI GGNCC 2 cut(s) 1481, 1511
PstNI CAGNNNCTG 2 cut(s) 49, 1166
PsuI RGATCY 2 cut(s) 784, 1432
RsaI GTAC 1 cut(s) 483
RsaNI GTAC 1 cut(s) 482
RseI CAYNNNNRTG 1 cut(s) 777
SaqAI TTAA 3 cut(s) 81, 831, 1374
SatI GCNGC 7 cut(s) 7, 16, 585, 853, 936, 1053, 1503
Sau3AI GATC 5 cut(s) 57, 226, 784, 1432, 1603
Sau96I GGNCC 2 cut(s) 1481, 1511
ScaI AGTACT 1 cut(s) 483
SchI GAGTC 4 cut(s) 1081, 1157, 1339, 1486
ScrFI CCNGG 4 cut(s) 50, 210, 318, 1406
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 5 cut(s) 435, 511, 582, 789, 1003
SfcI CTRYAG 1 cut(s) 603
SfuI TTCGAA 1 cut(s) 368
SinI GGWCC 1 cut(s) 1481
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 2 cut(s) 803, 1412
SmoI CTYRAG 2 cut(s) 803, 1412
SsiI CCGC 2 cut(s) 213, 308
SspMI CTAG 1 cut(s) 1320
StyD4I CCNGG 4 cut(s) 48, 208, 316, 1404
StyI CCWWGG 3 cut(s) 535, 1438, 1619
TaaI ACNGT 4 cut(s) 344, 640, 1271, 1385
TaiI ACGT 3 cut(s) 114, 453, 559
TaqI TCGA 8 cut(s) 72, 189, 286, 353, 368, 1257, 1287, 1498
TatI WGTACW 1 cut(s) 481
TfiI GAWTC 1 cut(s) 1171
Tru1I TTAA 3 cut(s) 81, 831, 1374
Tru9I TTAA 3 cut(s) 81, 831, 1374
TscAI CASTG 3 cut(s) 236, 349, 699
TseFI GTSAC 3 cut(s) 640, 793, 945
TseI GCWGC 7 cut(s) 6, 15, 584, 852, 935, 1052, 1502
Tsp45I GTSAC 3 cut(s) 640, 793, 945
TspDTI ATGAA 6 cut(s) 67, 377, 396, 761, 1235, 1305
TspGWI ACGGA 1 cut(s) 1037
TspRI CASTG 3 cut(s) 236, 349, 699
Van91I CCANNNNNTGG 2 cut(s) 49, 1112
VpaK11BI GGWCC 1 cut(s) 1481
XapI RAATTY 5 cut(s) 204, 251, 364, 389, 1656
XceI RCATGY 3 cut(s) 648, 670, 1191
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmnI GAANNNNTTC 1 cut(s) 958
XspI CTAG 1 cut(s) 1320
ZrmI AGTACT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.