Rh5AG189900

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003776
Physical Location & Seq
Forward (+)
21878086 .. 21885642
7557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG189900.1

Sequence Viewer

Length: 1653 bp
ATGTGCAGCAACTACTCGTGCGAGGCTGCCAGCCAGACCCTGGCATGGATCAACGCCATCATCGACTTCATAAAACCCTTCTCTTTCTTGACCAACGCTCACGTCGTCAATTTCTTCAAGGATAGACTGTGGGAGGCGATTGATGAGGAATGGATGGATTGCTTACGGGAGGAAGCAGTCGAAAATCTGCTCCAAATTCCTCGCGGAGCGGTGCAGGATCACTGGCCGGCTTCGCTGAAGGAATTTATTGTTACTTTGAGGTCTCTTGCGCTTCCTCGACAGCAGGCCGACTTGCAGGCGGTATTGCCTGGAATGAGCATGACTTCACTCAACAGTGTTCTTTCGACGGGCATGAATTCGAAGAAGAAACATGAAGTAGAAGTTTTTTCTGCTGTTGTCAGTTCAGTTGCAAACAGCATCGGTGCTCACGCAATAATTGATGTTGGTGCTGGTCAGGGGTATCTTGCACAAGTACTTTCCTTCCACTACCAACATTCGGTAATTGCAATTGATGCCTGCTCTCACCATGGAAGGGTTACCAATGCACGAGCAGAGCAAATCAAGAAACATTATGCTGCTCAGATGCGTAAATCTGTAGCAGGAAACAGGAGTTTGAATGTGCCTAAGACAGTCACATGCAATGTGATGTCTATTGACATGTTGAAAGCTTTGGCTGATACATCACTGCATAAAGATAATGCCTCTCTACTACAATCTTCCTGCAATGCAGATTGTGAAACATCATTGGTTCTTGCTGGGCTTCATGCTTGTGGAGATCTTTCGGTGACGATGCTCAAGACCTTTATGGAGTGCGAAGAGGTTAAAGCAGTTGTTAGTGTTGGCTGCTGTTACAACCTATTGTCTGAAGAGGGTTTCAACCATGTGGGGTCTCAATGTGGTTTTCCCATGAGTTGTGGTGTTAAAGCTGCTGGCTTGTCACTTGGGAAAAGTTCGCGTGACCTTGCTTGTCAAAGTGCAGAGAGATGGAGTTGTCTGGAAAATGATGCCGGTCTCCATAATTTTGAGTTGCATACTTTCCGCGCTGCTTTTCAGATGGTTTTGAGTCTATATTATCCTGAAGTTGTGATAACCAGTCCATCCATTGGGCGTCAAGGGAAGGCATTGCGTCGCCAGCAACGAAGGGGCATTCCAGACTCTGCGAATCCGAAAGAAAGCACATGTTCAACATTGCCTCAATTAGCTTGTAATATGAAAGAAAGTGGCTCTCACCTAAGAACCACAAAAATCGAAGAAGATGACAGTTTTGAGATGGAGTTCGAGACGACAGCCATAGGCAATGGACATTCATCTAGTAAGGCCACACAATGTGAGAGGACTCCACACATTAGCAAATATCCACTTTTTAAGAAATACTGTTTATCTGGATTATGTCGCCTGGGTCTTGAGCCTCCGAAGGATACAGATCTCCATGGAATATGGAAGAAAGTTCAGCCTTTTGCTGAACTTATTGGACCTTATTGGACTCTTCGAGCTGCTTTCGGGCCTCTTTTGGAAACTTTACTTTTGCTTGATAGATTATTGTTCCTACAAGAGCAAGGCAGTTCAATTGAAGTAGTCATGTTACCTATTTTTGATCCAGCATTATCCCCAAGGAATGTGGCCATTATTGCTAAAAAGTTGACACAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_32

Protein Analysis

550

Amino Acids

60.49

Weight (kDa)

6.53

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_32 PF13679 121 - 289 2.1e-27 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 40, 1103
AccBSI CCGCTC 1 cut(s) 209
AccII CGCG 3 cut(s) 204, 955, 1041
AciI CCGC 4 cut(s) 204, 209, 299, 1039
AclWI GGATC 3 cut(s) 56, 225, 1589
AcoI YGGCCR 2 cut(s) 224, 1620
AcsI RAATTY 4 cut(s) 195, 242, 355, 1647
AcuI CTGAAG 3 cut(s) 257, 885, 1098
AcyI GRCGYC 1 cut(s) 1108
AdeI CACNNNGTG 1 cut(s) 1328
AfaI GTAC 1 cut(s) 474
AfiI CCNNNNNNNGG 5 cut(s) 40, 45, 496, 532, 1103
AflIII ACRYGT 2 cut(s) 657, 1178
AgsI TTSAA 7 cut(s) 118, 616, 664, 877, 1185, 1566, 1571
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 3 cut(s) 39, 307, 1395
AluBI AGCT 4 cut(s) 668, 926, 1202, 1493
AluI AGCT 4 cut(s) 668, 926, 1202, 1493
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 4 cut(s) 267, 894, 1016, 1274
AlwI GGATC 3 cut(s) 56, 225, 1589
AlwNI CAGNNNCTG 2 cut(s) 40, 1157
AoxI GGCC 5 cut(s) 224, 285, 1317, 1502, 1620
ApeKI GCWGC 7 cut(s) 6, 26, 575, 843, 926, 1043, 1493
ApoI RAATTY 4 cut(s) 195, 242, 355, 1647
AspLEI GCGC 2 cut(s) 271, 1043
AspS9I GGNCC 2 cut(s) 1472, 1502
AsuHPI GGTGA 3 cut(s) 515, 796, 1220
AsuII TTCGAA 1 cut(s) 359
AvaII GGWCC 1 cut(s) 1472
BalI TGGCCA 1 cut(s) 1622
BauI CACGAG 2 cut(s) 16, 546
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 7 cut(s) 13, 18, 562, 830, 913, 1030, 1480
BccI CCATC 6 cut(s) 65, 148, 978, 1048, 1105, 1264
BciT130I CCWGG 3 cut(s) 41, 309, 1397
BciVI GTATCC 1 cut(s) 1411
BcoDI GTCTC 4 cut(s) 267, 894, 1016, 1274
BfaI CTAG 1 cut(s) 1311
BfmI CTRYAG 1 cut(s) 594
BfuI GTATCC 1 cut(s) 1411
BglII AGATCT 2 cut(s) 775, 1423
BisI GCNGC 7 cut(s) 7, 27, 576, 844, 927, 1044, 1494
BlsI GCNGC 7 cut(s) 8, 28, 577, 845, 928, 1045, 1495
BmcAI AGTACT 1 cut(s) 474
Bme1390I CCNGG 3 cut(s) 41, 309, 1397
Bme18I GGWCC 1 cut(s) 1472
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 1472, 1502
BmrFI CCNGG 3 cut(s) 41, 309, 1397
BmsI GCATC 5 cut(s) 426, 502, 573, 780, 994
Bpu14I TTCGAA 1 cut(s) 359
BpuEI CTTGAG 2 cut(s) 779, 1424
BsaBI GATNNNNATC 1 cut(s) 1422
BsaHI GRCGYC 1 cut(s) 1108
BsaI GGTCTC 3 cut(s) 267, 894, 1016
BsaJI CCNNGG 5 cut(s) 39, 526, 1396, 1429, 1610
Bsc4I CCNNNNNNNGG 5 cut(s) 40, 45, 496, 532, 1103
Bse118I RCCGGY 2 cut(s) 226, 1007
Bse1I ACTGG 2 cut(s) 227, 1092
Bse3DI GCAATG 5 cut(s) 646, 730, 1121, 1187, 1303
Bse8I GATNNNNATC 1 cut(s) 1422
BseBI CCWGG 3 cut(s) 41, 309, 1397
BseDI CCNNGG 5 cut(s) 39, 526, 1396, 1429, 1610
BseGI GGATG 2 cut(s) 159, 1097
BseJI GATNNNNATC 1 cut(s) 1422
BseLI CCNNNNNNNGG 5 cut(s) 40, 45, 496, 532, 1103
BseMI GCAATG 5 cut(s) 646, 730, 1121, 1187, 1303
BseMII CTCAG 1 cut(s) 593
BseNI ACTGG 2 cut(s) 227, 1092
BseXI GCAGC 7 cut(s) 13, 18, 562, 830, 913, 1030, 1480
BseYI CCCAGC 1 cut(s) 755
BsgI GTGCAG 3 cut(s) 25, 233, 996
Bsh1236I CGCG 3 cut(s) 204, 955, 1041
BshFI GGCC 5 cut(s) 226, 287, 1319, 1504, 1622
BsiHKAI GWGCWC 1 cut(s) 427
BsiSI CCGG 2 cut(s) 227, 1008
BslI CCNNNNNNNGG 5 cut(s) 40, 45, 496, 532, 1103
BsmAI GTCTC 4 cut(s) 267, 894, 1016, 1274
BsmBI CGTCTC 1 cut(s) 1274
BsmI GAATGC 1 cut(s) 1146
BsnI GGCC 5 cut(s) 226, 287, 1319, 1504, 1622
Bso31I GGTCTC 3 cut(s) 267, 894, 1016
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 5 cut(s) 48, 217, 775, 1423, 1594
Bsp19I CCATGG 2 cut(s) 526, 1429
BspACI CCGC 4 cut(s) 204, 209, 299, 1039
BspANI GGCC 5 cut(s) 226, 287, 1319, 1504, 1622
BspCNI CTCAG 1 cut(s) 592
BspFNI CGCG 3 cut(s) 204, 955, 1041
BspPI GGATC 3 cut(s) 56, 225, 1589
BspT104I TTCGAA 1 cut(s) 359
BspTNI GGTCTC 3 cut(s) 267, 894, 1016
BsrBI CCGCTC 1 cut(s) 209
BsrDI GCAATG 5 cut(s) 646, 730, 1121, 1187, 1303
BsrFI RCCGGY 2 cut(s) 226, 1007
BsrI ACTGG 2 cut(s) 227, 1092
BssAI RCCGGY 2 cut(s) 226, 1007
BssECI CCNNGG 5 cut(s) 39, 526, 1396, 1429, 1610
BssMI GATC 5 cut(s) 48, 217, 775, 1423, 1594
BssNI GRCGYC 1 cut(s) 1108
BssSI CACGAG 2 cut(s) 16, 546
BssT1I CCWWGG 3 cut(s) 526, 1429, 1610
Bst2BI CACGAG 2 cut(s) 16, 546
Bst2UI CCWGG 3 cut(s) 41, 309, 1397
Bst4CI ACNGT 5 cut(s) 129, 335, 631, 1262, 1376
Bst6I CTCTTC 3 cut(s) 810, 861, 1491
BstACI GRCGYC 1 cut(s) 1108
BstAPI GCANNNNNTGC 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 7 cut(s) 31, 228, 285, 297, 517, 931, 1133
BstDEI CTNAG 3 cut(s) 579, 624, 1232
BstDSI CCRYGG 2 cut(s) 526, 1429
BstEII GGTNACC 1 cut(s) 535
BstF5I GGATG 2 cut(s) 159, 1097
BstFNI CGCG 3 cut(s) 204, 955, 1041
BstHHI GCGC 2 cut(s) 271, 1043
BstKTI GATC 5 cut(s) 51, 220, 778, 1426, 1597
BstMAI GTCTC 4 cut(s) 267, 894, 1016, 1274
BstMBI GATC 5 cut(s) 48, 217, 775, 1423, 1594
BstMWI GCNNNNNNNGC 4 cut(s) 232, 512, 1132, 1628
BstNI CCWGG 3 cut(s) 41, 309, 1397
BstNSI RCATGY 3 cut(s) 639, 661, 1182
BstPI GGTNACC 1 cut(s) 535
BstSCI CCNGG 3 cut(s) 39, 307, 1395
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 3 cut(s) 204, 955, 1041
BstV1I GCAGC 7 cut(s) 13, 18, 562, 830, 913, 1030, 1480
BstX2I RGATCY 2 cut(s) 775, 1423
BstYI RGATCY 2 cut(s) 775, 1423
BsuI GTATCC 1 cut(s) 1411
BsuRI GGCC 5 cut(s) 226, 287, 1319, 1504, 1622
BtgI CCRYGG 2 cut(s) 526, 1429
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 2 cut(s) 159, 1097
BtsI GCAGTG 1 cut(s) 683
BtsIMutI CAGTG 3 cut(s) 220, 340, 683
Cac8I GCNNGC 7 cut(s) 31, 228, 285, 297, 517, 931, 1133
CaiI CAGNNNCTG 2 cut(s) 40, 1157
CfoI GCGC 2 cut(s) 271, 1043
Cfr10I RCCGGY 2 cut(s) 226, 1007
Cfr13I GGNCC 2 cut(s) 1472, 1502
CseI GACGC 2 cut(s) 1097, 1115
Csp6I GTAC 1 cut(s) 473
CspCI CAANNNNNGTGG 2 cut(s) 1599, 1634
CviQI GTAC 1 cut(s) 473
DdeI CTNAG 3 cut(s) 579, 624, 1232
DpnI GATC 5 cut(s) 50, 219, 777, 1425, 1596
DpnII GATC 5 cut(s) 48, 217, 775, 1423, 1594
DraIII CACNNNGTG 1 cut(s) 1328
EaeI YGGCCR 2 cut(s) 224, 1620
Eam1104I CTCTTC 3 cut(s) 810, 861, 1491
EarI CTCTTC 3 cut(s) 810, 861, 1491
Eco130I CCWWGG 3 cut(s) 526, 1429, 1610
Eco31I GGTCTC 3 cut(s) 267, 894, 1016
Eco47I GGWCC 1 cut(s) 1472
Eco57I CTGAAG 3 cut(s) 257, 885, 1098
Eco91I GGTNACC 1 cut(s) 535
EcoO65I GGTNACC 1 cut(s) 535
EcoRI GAATTC 1 cut(s) 355
EcoRII CCWGG 3 cut(s) 39, 307, 1395
EcoT14I CCWWGG 3 cut(s) 526, 1429, 1610
ErhI CCWWGG 3 cut(s) 526, 1429, 1610
Esp3I CGTCTC 1 cut(s) 1274
Fnu4HI GCNGC 7 cut(s) 7, 27, 576, 844, 927, 1044, 1494
FokI GGATG 2 cut(s) 166, 1084
Fsp4HI GCNGC 7 cut(s) 7, 27, 576, 844, 927, 1044, 1494
FspBI CTAG 1 cut(s) 1311
GlaI GCGC 2 cut(s) 270, 1042
GluI GCNGC 7 cut(s) 7, 27, 576, 844, 927, 1044, 1494
GsaI CCCAGC 1 cut(s) 759
HaeIII GGCC 5 cut(s) 226, 287, 1319, 1504, 1622
HapII CCGG 2 cut(s) 227, 1008
HgaI GACGC 2 cut(s) 1097, 1115
HhaI GCGC 2 cut(s) 271, 1043
Hin1I GRCGYC 1 cut(s) 1108
Hin6I GCGC 2 cut(s) 269, 1041
HinP1I GCGC 2 cut(s) 269, 1041
HincII GTYRAC 1 cut(s) 1641
HindII GTYRAC 1 cut(s) 1641
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 5 cut(s) 1063, 1154, 1162, 1336, 1483
HpaII CCGG 2 cut(s) 227, 1008
HphI GGTGA 3 cut(s) 515, 796, 1220
Hpy166II GTNNAC 1 cut(s) 1641
Hpy188I TCNGA 5 cut(s) 582, 865, 1053, 1167, 1413
Hpy188III TCNNGA 9 cut(s) 88, 562, 796, 995, 1076, 1151, 1279, 1383, 1403
Hpy8I GTNNAC 1 cut(s) 1641
Hpy99I CGWCG 3 cut(s) 107, 349, 1131
HpyAV CCTTC 7 cut(s) 88, 232, 490, 525, 1111, 1134, 1408
HpyCH4III ACNGT 5 cut(s) 129, 335, 631, 1262, 1376
HpyCH4IV ACGT 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 4 cut(s) 232, 512, 1132, 1628
HpyF3I CTNAG 3 cut(s) 579, 624, 1232
HpySE526I ACGT 1 cut(s) 102
Hsp92I GRCGYC 1 cut(s) 1108
HspAI GCGC 2 cut(s) 269, 1041
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
Kzo9I GATC 5 cut(s) 48, 217, 775, 1423, 1594
LmnI GCTCC 2 cut(s) 195, 206
Lsp1109I GCAGC 7 cut(s) 13, 18, 562, 830, 913, 1030, 1480
LweI GCATC 5 cut(s) 426, 502, 573, 780, 994
MaeI CTAG 1 cut(s) 1311
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 8 cut(s) 250, 535, 631, 784, 848, 936, 956, 1581
MalI GATC 5 cut(s) 50, 219, 777, 1425, 1596
MbiI CCGCTC 1 cut(s) 209
MboI GATC 5 cut(s) 48, 217, 775, 1423, 1594
MfeI CAATTG 2 cut(s) 507, 1566
MflI RGATCY 2 cut(s) 775, 1423
MhlI GDGCHC 1 cut(s) 427
MlsI TGGCCA 1 cut(s) 1622
MluNI TGGCCA 1 cut(s) 1622
MlyI GAGTC 4 cut(s) 1072, 1148, 1330, 1477
Mox20I TGGCCA 1 cut(s) 1622
MroNI GCCGGC 1 cut(s) 226
MscI TGGCCA 1 cut(s) 1622
MseI TTAA 3 cut(s) 822, 921, 1365
MslI CAYNNNNRTG 1 cut(s) 768
Msp20I TGGCCA 1 cut(s) 1622
MspI CCGG 2 cut(s) 227, 1008
MspR9I CCNGG 3 cut(s) 41, 309, 1397
MunI CAATTG 2 cut(s) 507, 1566
Mva1269I GAATGC 1 cut(s) 1146
MvaI CCWGG 3 cut(s) 41, 309, 1397
MvnI CGCG 3 cut(s) 204, 955, 1041
MwoI GCNNNNNNNGC 4 cut(s) 232, 512, 1132, 1628
NaeI GCCGGC 1 cut(s) 228
NcoI CCATGG 2 cut(s) 526, 1429
NdeII GATC 5 cut(s) 48, 217, 775, 1423, 1594
NgoMIV GCCGGC 1 cut(s) 226
NmuCI GTSAC 4 cut(s) 631, 784, 936, 956
NspI RCATGY 3 cut(s) 639, 661, 1182
NspV TTCGAA 1 cut(s) 359
PciI ACATGT 2 cut(s) 657, 1178
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 102, 1135
PctI GAATGC 1 cut(s) 1146
PdiI GCCGGC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 1162
PflMI CCANNNNNTGG 2 cut(s) 40, 1103
PkrI GCNGC 7 cut(s) 8, 28, 577, 845, 928, 1045, 1495
PleI GAGTC 4 cut(s) 1071, 1148, 1330, 1477
PpsI GAGTC 4 cut(s) 1071, 1148, 1330, 1477
PscI ACATGT 2 cut(s) 657, 1178
Psp6I CCWGG 3 cut(s) 39, 307, 1395
PspEI GGTNACC 1 cut(s) 535
PspFI CCCAGC 1 cut(s) 755
PspGI CCWGG 3 cut(s) 39, 307, 1395
PspPI GGNCC 2 cut(s) 1472, 1502
PstNI CAGNNNCTG 2 cut(s) 40, 1157
PsuI RGATCY 2 cut(s) 775, 1423
RsaI GTAC 1 cut(s) 474
RsaNI GTAC 1 cut(s) 473
RseI CAYNNNNRTG 1 cut(s) 768
SaqAI TTAA 3 cut(s) 822, 921, 1365
SatI GCNGC 7 cut(s) 7, 27, 576, 844, 927, 1044, 1494
Sau3AI GATC 5 cut(s) 48, 217, 775, 1423, 1594
Sau96I GGNCC 2 cut(s) 1472, 1502
ScaI AGTACT 1 cut(s) 474
SchI GAGTC 4 cut(s) 1072, 1148, 1330, 1477
ScrFI CCNGG 3 cut(s) 41, 309, 1397
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 5 cut(s) 426, 502, 573, 780, 994
SfcI CTRYAG 1 cut(s) 594
SfuI TTCGAA 1 cut(s) 359
SinI GGWCC 1 cut(s) 1472
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 2 cut(s) 794, 1403
SmoI CTYRAG 2 cut(s) 794, 1403
SsiI CCGC 4 cut(s) 204, 209, 299, 1039
SspMI CTAG 1 cut(s) 1311
StyD4I CCNGG 3 cut(s) 39, 307, 1395
StyI CCWWGG 3 cut(s) 526, 1429, 1610
TaaI ACNGT 5 cut(s) 129, 335, 631, 1262, 1376
TaiI ACGT 1 cut(s) 105
TaqI TCGA 8 cut(s) 63, 180, 277, 344, 359, 1248, 1278, 1489
TatI WGTACW 1 cut(s) 472
TfiI GAWTC 1 cut(s) 1162
Tru1I TTAA 3 cut(s) 822, 921, 1365
Tru9I TTAA 3 cut(s) 822, 921, 1365
TscAI CASTG 3 cut(s) 227, 340, 690
TseFI GTSAC 4 cut(s) 631, 784, 936, 956
TseI GCWGC 7 cut(s) 6, 26, 575, 843, 926, 1043, 1493
Tsp45I GTSAC 4 cut(s) 631, 784, 936, 956
TspDTI ATGAA 6 cut(s) 58, 368, 387, 752, 1226, 1296
TspRI CASTG 3 cut(s) 227, 340, 690
Van91I CCANNNNNTGG 2 cut(s) 40, 1103
VpaK11BI GGWCC 1 cut(s) 1472
XapI RAATTY 4 cut(s) 195, 242, 355, 1647
XceI RCATGY 3 cut(s) 639, 661, 1182
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XspI CTAG 1 cut(s) 1311
ZrmI AGTACT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.