Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0013190

Physical Location & Seq

Genomic Coordinates Forward (+)

36365552 .. 36368856

Gene Structure 3305 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1230200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGGTATTTTCAGCAAATGCAAGCCCTATAAACCCCAATCGTCGGTTTGCCACCACCACCCCACAACCACCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTATCTATCTTCTCTGCCTCCGTTTTTCTTCCTCCTCCTTTCTTCTTCTCCGATCCGTCTCCATGGAACCAGTAAACGCGTGGGGCAACTCCTCCCTGGAGAGCGTGGACCCCGAGATCCACGACCTCATCGAGAAGGAGAAGCGCCGCCAATGCCGAGGAATCGAGCTCATTGCCTCCGAAAACTTCACCTCCTTCGCCGTCATTGAGGCCCTCGGCAGCGCCCTCACCAACAAGTACTCCGAAGGCATGCCCGGCAACCGCTACTACGGAGGCAACGAGTTCATCGACGAGATCGAGAACATGTGCCGCTCGCGCGCCCTCCAGGCCTTCCACCTCGACGCCGCCAAATGGGGCGTCAACGTTCAGCCCTACTCCGGTTCTCCCGCCAACTTCGCCGCATACACCGCCGTCCTCGAGCCCCATGACCGCATCATGGGATTGGACCTTCCCTCTGGTGGTCACCTGACCCACGGGTACTACACTTCCGGAGGGAAGAAGATCTCGGCGACCTCGATTTACTTCGAGAGTCTGCCTTACAAGGTCAATTCCTCAACTGGGTTCATCGATTACGACAAGTTGGAGGAGAAGGCCTTGGATTTCAGGCCAAAATTGATAATTTGCGGCGGCAGTGCGTACCCCAGGGACTGGGATTACGCAAGGTTCCGCGCCGTTGCGGATAAGTGCGGTGCTCTTTTGCTCTGCGACATGGCTCACATTAGCGGCCTTGTTGCTGCTCAGGAAGCTGCAAATCCTTTCGAGTACTGTGACATTGTCACAACCACAACCCACAAGAGCTTGAGGGGTCCTAGGGCTGGTATGATCTTCTACAGGAAGGGACCAAAACCACCCAAGAAGGGCCAGCCCGAGGGTGCAGTTTACGACTTTGAAGACAAGGTCAACTTTGCTGTTTTTCCCTCCCTTCAAGGTGGTCCTCACAATCACCAAATCGGTGCCCTAGCTGTTGCTCTTAAGCAGGCCATGACACCCGGGTTCAAGGCATACGCCAAACAAGTCAAGGCCAACGCTGTTGCCCTTGGAAACTACCTGATGAGCAAGGATTACAAGCTTGTCACAGGAGGAACAGAGAACCACCTTGTTCTGTGGGATCTCAGGCCTCTTGGTTTGACAGGCAACAAGGTTGAGAAGCTTTGTGACCTGTGCAACATTACTGTGAACAAGAATGCTGTTTTTGGTGACAGCAGTGCCTTGGCTCCTGGCGGAGTAAGAATCGGTGCCCCGGCTATGACCTCGAGAGGTTTGGTTGAGAAGGATTTTGAGAAGATAGGGGAATTCCTTCACAGGGCCGTAACTCTCACCTTGAAAATCCAGAAGGAGCATGGAAAACTACTCAAGGACTTCAACAAGGGTTTGGTGAACAACAAGGACATTGAAGAACTCAAGGCCGATGTCGAGAAGTTCTCAGCCTCGTTTGACATGCCCGGCTTCTCCTTGGCCGACATGAAGTACAAGGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000741 GO:0003674 GO:0003824 GO:0004372 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006730 GO:0006996 GO:0006997 GO:0007275 GO:0007623 GO:0008150 GO:0008152 GO:0009506 GO:0009553 GO:0009555 GO:0009559 GO:0009561 GO:0009987 GO:0010035 GO:0010038 GO:0010197 GO:0016020 GO:0016043 GO:0016740 GO:0016741 GO:0016742 GO:0030054 GO:0030154 GO:0032501 GO:0032502 GO:0042221 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048229 GO:0048284 GO:0048511 GO:0048856 GO:0048869 GO:0050896 GO:0055044 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00260 ko00460 ko00630 ko00670 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko01523 map00260 map00460 map00630 map00670 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map01523

Pfam Domains

Protein Families

SHMT

Protein Analysis

528

Amino Acids

57.79

Weight (kDa)

8.38

Isoelectric Point (pI)

41.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SHMT	PF00464	68 - 467	7.9e-188	Serine hydroxymethyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013190)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13890 AT4G13890 AT4G13930
fragaria_vesca	FvH4_4g13290 FvH4_4g13290
malus_domestica	MD05G1230200.v1.1
prunus_persica	Prupe.1G165100_v2.0.a1
pyrus_communis	pycom04g01360 pycom420g00990
rosa_chinensis	RchiOBHm_Chr4g0413581
rosa_laevigata	RLG00000008211
rosa_multiflora	Rmu_sc0008298.1_g000001
rosa_roxburghii	Rroxscaffold_5G00357460
rosa_rugosa	Rorug04G0121000
rosa_samantha	Rh4AG180800 Rh4BG180000 Rh4CG191900
rosa_wichuraiana	Rw4G015100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	1058, 1340
AccB7I	CCANNNNNTGG	1 cut(s)	753
AccBSI	CCGCTC	1 cut(s)	417
AccII	CGCG	4 cut(s)	185, 421, 423, 774
AccIII	TCCGGA	1 cut(s)	593
AclI	AACGTT	1 cut(s)	468
AclWI	GGATC	3 cut(s)	153, 217, 1221
AcoI	YGGCCR	1 cut(s)	1560
AcsI	RAATTY	1 cut(s)	1397
AcyI	GRCGYC	2 cut(s)	447, 462
AfaI	GTAC	5 cut(s)	344, 584, 743, 869, 1574
AflII	CTTAAG	1 cut(s)	1076
AflIII	ACRYGT	2 cut(s)	183, 408
AgsI	TTSAA	6 cut(s)	995, 1031, 1102, 1429, 1468, 1499
AjnI	CCWGG	4 cut(s)	201, 429, 746, 1321
AluBI	AGCT	6 cut(s)	274, 851, 903, 1067, 1174, 1255
AluI	AGCT	6 cut(s)	274, 851, 903, 1067, 1174, 1255
Alw21I	GWGCWC	2 cut(s)	276, 799
Alw26I	GTCTC	1 cut(s)	169
AlwI	GGATC	3 cut(s)	153, 217, 1221
AlwNI	CAGNNNCTG	1 cut(s)	753
Ama87I	CYCGRG	5 cut(s)	218, 521, 971, 1094, 1357
Aor13HI	TCCGGA	1 cut(s)	593
ApeKI	GCWGC	3 cut(s)	324, 839, 851
ApoI	RAATTY	1 cut(s)	1397
ArsI	GACNNNNNNTTYG	2 cut(s)	447, 479
Asp700I	GAANNNNTTC	1 cut(s)	1401
AspA2I	CCTAGG	1 cut(s)	914
AspLEI	GCGC	5 cut(s)	252, 329, 423, 425, 776
AspS9I	GGNCC	8 cut(s)	214, 316, 550, 911, 944, 964, 1037, 1410
AsuC2I	CCSGG	5 cut(s)	360, 1095, 1096, 1346, 1548
AsuHPI	GGTGA	7 cut(s)	286, 325, 560, 1040, 1313, 1414, 1492
AvaI	CYCGRG	5 cut(s)	218, 521, 971, 1094, 1357
AvaII	GGWCC	5 cut(s)	214, 550, 911, 944, 1037
AvrII	CCTAGG	1 cut(s)	914
BaeGI	GKGCMC	2 cut(s)	1063, 1345
BanI	GGYRCC	2 cut(s)	1058, 1340
BanII	GRGCYC	2 cut(s)	276, 528
BbsI	GAAGAC	1 cut(s)	1002
Bbv12I	GWGCWC	2 cut(s)	276, 799
BbvI	GCAGC	3 cut(s)	336, 826, 838
BceAI	ACGGC	4 cut(s)	290, 500, 761, 1397
BcgI	CGANNNNNNTGC	2 cut(s)	260, 294
BciT130I	CCWGG	4 cut(s)	203, 431, 748, 1323
BcnI	CCSGG	5 cut(s)	360, 1095, 1096, 1346, 1548
BcoDI	GTCTC	1 cut(s)	169
BfaI	CTAG	2 cut(s)	915, 1064
BfmI	CTRYAG	1 cut(s)	934
BfoI	RGCGCY	2 cut(s)	253, 330
BfrI	CTTAAG	1 cut(s)	1076
BglI	GCCNNNNNGGC	1 cut(s)	431
BglII	AGATCT	1 cut(s)	606
BlnI	CCTAGG	1 cut(s)	914
BmcAI	AGTACT	2 cut(s)	344, 869
Bme1390I	CCNGG	9 cut(s)	203, 360, 431, 748, 1095, 1096, 1323, 1346, 1548
Bme18I	GGWCC	5 cut(s)	214, 550, 911, 944, 1037
BmeT110I	CYCGRG	5 cut(s)	218, 521, 971, 1094, 1357
BmgT120I	GGNCC	8 cut(s)	214, 316, 550, 911, 944, 964, 1037, 1410
BmiI	GGNNCC	8 cut(s)	174, 216, 770, 912, 945, 1060, 1320, 1342
BmrFI	CCNGG	9 cut(s)	203, 360, 431, 748, 1095, 1096, 1323, 1346, 1548
BmrI	ACTGGG	2 cut(s)	672, 763
BmsI	GCATC	1 cut(s)	546
BmuI	ACTGGG	2 cut(s)	672, 763
BpiI	GAAGAC	1 cut(s)	1002
BplI	GAGNNNNNCTC	2 cut(s)	1511, 1543
BpmI	CTGGAG	2 cut(s)	224, 413
Bpu10I	CCTNAGC	1 cut(s)	843
BpuEI	CTTGAG	3 cut(s)	925, 1442, 1490
BpuMI	CCSGG	5 cut(s)	360, 1095, 1096, 1346, 1548
Bsa29I	ATCGAT	1 cut(s)	672
BsaHI	GRCGYC	2 cut(s)	447, 462
BsaWI	WCCGGW	2 cut(s)	482, 593
BsaXI	ACNNNNNCTCC	4 cut(s)	281, 311, 329, 359
Bse1I	ACTGG	3 cut(s)	176, 667, 758
Bse3DI	GCAATG	1 cut(s)	276
BseAI	TCCGGA	1 cut(s)	593
BseBI	CCWGG	4 cut(s)	203, 431, 748, 1323
BseCI	ATCGAT	1 cut(s)	672
BseMI	GCAATG	1 cut(s)	276
BseMII	CTCAG	3 cut(s)	857, 1231, 1542
BseNI	ACTGG	3 cut(s)	176, 667, 758
BsePI	GCGCGC	1 cut(s)	421
BseRI	GAGGAG	3 cut(s)	130, 187, 704
BseSI	GKGCMC	2 cut(s)	1063, 1345
BseXI	GCAGC	3 cut(s)	336, 826, 838
BsgI	GTGCAG	1 cut(s)	999
Bsh1236I	CGCG	4 cut(s)	185, 421, 423, 774
BshNI	GGYRCC	2 cut(s)	1058, 1340
BshVI	ATCGAT	1 cut(s)	672
BsiHKAI	GWGCWC	2 cut(s)	276, 799
BsiHKCI	CYCGRG	5 cut(s)	218, 521, 971, 1094, 1357
BsiSI	CCGG	6 cut(s)	360, 483, 594, 1095, 1346, 1548
BslFI	GGGAC	2 cut(s)	764, 957
BsmAI	GTCTC	1 cut(s)	169
BsmBI	CGTCTC	1 cut(s)	169
BsmFI	GGGAC	2 cut(s)	764, 957
BsmI	GAATGC	1 cut(s)	1294
BsoBI	CYCGRG	5 cut(s)	218, 521, 971, 1094, 1357
Bsp1286I	GDGCHC	5 cut(s)	276, 528, 799, 1063, 1345
Bsp13I	TCCGGA	1 cut(s)	593
Bsp143I	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
Bsp19I	CCATGG	1 cut(s)	168
BspCNI	CTCAG	3 cut(s)	856, 1230, 1541
BspDI	ATCGAT	1 cut(s)	672
BspEI	TCCGGA	1 cut(s)	593
BspFNI	CGCG	4 cut(s)	185, 421, 423, 774
BspLI	GGNNCC	8 cut(s)	174, 216, 770, 912, 945, 1060, 1320, 1342
BspPI	GGATC	3 cut(s)	153, 217, 1221
BspT107I	GGYRCC	2 cut(s)	1058, 1340
BspTI	CTTAAG	1 cut(s)	1076
BsrBI	CCGCTC	1 cut(s)	417
BsrDI	GCAATG	1 cut(s)	276
BsrI	ACTGG	3 cut(s)	176, 667, 758
BssHII	GCGCGC	1 cut(s)	421
BssMI	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
BssNI	GRCGYC	2 cut(s)	447, 462
BssT1I	CCWWGG	6 cut(s)	168, 699, 914, 1141, 1314, 1557
Bst2UI	CCWGG	4 cut(s)	203, 431, 748, 1323
Bst4CI	ACNGT	2 cut(s)	872, 1279
BstACI	GRCGYC	2 cut(s)	447, 462
BstAFI	CTTAAG	1 cut(s)	1076
BstC8I	GCNNGC	6 cut(s)	22, 356, 419, 423, 968, 1083
BstDEI	CTNAG	3 cut(s)	843, 1217, 1528
BstDSI	CCRYGG	2 cut(s)	168, 577
BstEII	GGTNACC	1 cut(s)	566
BstFNI	CGCG	4 cut(s)	185, 421, 423, 774
BstH2I	RGCGCY	2 cut(s)	253, 330
BstHHI	GCGC	5 cut(s)	252, 329, 423, 425, 776
BstKTI	GATC	6 cut(s)	161, 225, 402, 609, 930, 1216
BstMAI	GTCTC	1 cut(s)	169
BstMBI	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
BstMWI	GCNNNNNNNGC	7 cut(s)	258, 360, 420, 431, 500, 512, 848
BstNI	CCWGG	4 cut(s)	203, 431, 748, 1323
BstNSI	RCATGY	3 cut(s)	358, 412, 1546
BstPI	GGTNACC	1 cut(s)	566
BstSCI	CCNGG	9 cut(s)	201, 358, 429, 746, 1093, 1094, 1321, 1344, 1546
BstSFI	CTRYAG	1 cut(s)	934
BstSLI	GKGCMC	2 cut(s)	1063, 1345
BstUI	CGCG	4 cut(s)	185, 421, 423, 774
BstV1I	GCAGC	3 cut(s)	336, 826, 838
BstV2I	GAAGAC	1 cut(s)	1002
BstX2I	RGATCY	3 cut(s)	222, 606, 1213
BstYI	RGATCY	3 cut(s)	222, 606, 1213
Bsu15I	ATCGAT	1 cut(s)	672
BsuTUI	ATCGAT	1 cut(s)	672
BtgI	CCRYGG	2 cut(s)	168, 577
BtsI	GCAGTG	2 cut(s)	742, 1315
BtsIMutI	CAGTG	2 cut(s)	742, 1315
Cac8I	GCNNGC	6 cut(s)	22, 356, 419, 423, 968, 1083
CaiI	CAGNNNCTG	1 cut(s)	753
CfoI	GCGC	5 cut(s)	252, 329, 423, 425, 776
Cfr13I	GGNCC	8 cut(s)	214, 316, 550, 911, 944, 964, 1037, 1410
Cfr9I	CCCGGG	1 cut(s)	1094
ClaI	ATCGAT	1 cut(s)	672
CseI	GACGC	2 cut(s)	451, 455
Csp6I	GTAC	5 cut(s)	343, 583, 742, 868, 1573
CviQI	GTAC	5 cut(s)	343, 583, 742, 868, 1573
DdeI	CTNAG	3 cut(s)	843, 1217, 1528
DpnI	GATC	6 cut(s)	160, 224, 401, 608, 929, 1215
DpnII	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
EaeI	YGGCCR	1 cut(s)	1560
EciI	GGCGGA	1 cut(s)	1341
Ecl136II	GAGCTC	1 cut(s)	274
Eco130I	CCWWGG	6 cut(s)	168, 699, 914, 1141, 1314, 1557
Eco147I	AGGCCT	3 cut(s)	434, 698, 1222
Eco24I	GRGCYC	2 cut(s)	276, 528
Eco47I	GGWCC	5 cut(s)	214, 550, 911, 944, 1037
Eco53kI	GAGCTC	1 cut(s)	274
Eco88I	CYCGRG	5 cut(s)	218, 521, 971, 1094, 1357
Eco91I	GGTNACC	1 cut(s)	566
EcoICRI	GAGCTC	1 cut(s)	274
EcoO109I	RGGNCCY	2 cut(s)	316, 911
EcoO65I	GGTNACC	1 cut(s)	566
EcoRI	GAATTC	1 cut(s)	1397
EcoRII	CCWGG	4 cut(s)	201, 429, 746, 1321
EcoT14I	CCWWGG	6 cut(s)	168, 699, 914, 1141, 1314, 1557
EcoT38I	GRGCYC	2 cut(s)	276, 528
ErhI	CCWWGG	6 cut(s)	168, 699, 914, 1141, 1314, 1557
Esp3I	CGTCTC	1 cut(s)	169
FalI	AAGNNNNNCTT	2 cut(s)	992, 1024
FaqI	GGGAC	2 cut(s)	764, 957
FauI	CCCGC	1 cut(s)	499
FriOI	GRGCYC	2 cut(s)	276, 528
FspBI	CTAG	2 cut(s)	915, 1064
GlaI	GCGC	5 cut(s)	251, 328, 422, 424, 775
GsuI	CTGGAG	2 cut(s)	224, 413
HaeII	RGCGCY	2 cut(s)	253, 330
HapII	CCGG	6 cut(s)	360, 483, 594, 1095, 1346, 1548
HgaI	GACGC	2 cut(s)	451, 455
HhaI	GCGC	5 cut(s)	252, 329, 423, 425, 776
Hin1I	GRCGYC	2 cut(s)	447, 462
Hin6I	GCGC	5 cut(s)	250, 327, 421, 423, 774
HinP1I	GCGC	5 cut(s)	250, 327, 421, 423, 774
HincII	GTYRAC	2 cut(s)	466, 1006
HindII	GTYRAC	2 cut(s)	466, 1006
HindIII	AAGCTT	2 cut(s)	1172, 1253
HinfI	GANTC	3 cut(s)	267, 634, 1335
HpaII	CCGG	6 cut(s)	360, 483, 594, 1095, 1346, 1548
HphI	GGTGA	7 cut(s)	286, 325, 560, 1040, 1313, 1414, 1492
Hpy166II	GTNNAC	7 cut(s)	181, 214, 466, 985, 1006, 1282, 1483
Hpy188I	TCNGA	3 cut(s)	158, 286, 349
Hpy188III	TCNNGA	8 cut(s)	238, 403, 594, 631, 845, 1359, 1435, 1519
Hpy8I	GTNNAC	7 cut(s)	181, 214, 466, 985, 1006, 1282, 1483
Hpy99I	CGWCG	3 cut(s)	45, 398, 449
HpyCH4III	ACNGT	2 cut(s)	872, 1279
HpyCH4IV	ACGT	1 cut(s)	468
HpyCH4V	TGCA	4 cut(s)	20, 854, 980, 1269
HpyF10VI	GCNNNNNNNGC	7 cut(s)	258, 360, 420, 431, 500, 512, 848
HpyF3I	CTNAG	3 cut(s)	843, 1217, 1528
HpySE526I	ACGT	1 cut(s)	468
Hsp92I	GRCGYC	2 cut(s)	447, 462
HspAI	GCGC	5 cut(s)	250, 327, 421, 423, 774
Kpn2I	TCCGGA	1 cut(s)	593
Kzo9I	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
LmnI	GCTCC	2 cut(s)	1324, 1441
Lsp1109I	GCAGC	3 cut(s)	336, 826, 838
LweI	GCATC	1 cut(s)	546
MaeI	CTAG	2 cut(s)	915, 1064
MaeII	ACGT	1 cut(s)	468
MaeIII	GTNAC	7 cut(s)	566, 872, 880, 1177, 1259, 1301, 1414
MalI	GATC	6 cut(s)	160, 224, 401, 608, 929, 1215
MbiI	CCGCTC	1 cut(s)	417
MboI	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
MflI	RGATCY	3 cut(s)	222, 606, 1213
MhlI	GDGCHC	5 cut(s)	276, 528, 799, 1063, 1345
MluCI	AATT	4 cut(s)	652, 716, 723, 1397
MluI	ACGCGT	1 cut(s)	183
MlyI	GAGTC	1 cut(s)	643
MmeI	TCCRAC	1 cut(s)	666
MroI	TCCGGA	1 cut(s)	593
MroXI	GAANNNNTTC	1 cut(s)	1401
MseI	TTAA	2 cut(s)	1077, 1582
MslI	CAYNNNNRTG	1 cut(s)	1277
MspCI	CTTAAG	1 cut(s)	1076
MspI	CCGG	6 cut(s)	360, 483, 594, 1095, 1346, 1548
MspR9I	CCNGG	9 cut(s)	203, 360, 431, 748, 1095, 1096, 1323, 1346, 1548
Mva1269I	GAATGC	1 cut(s)	1294
MvaI	CCWGG	4 cut(s)	203, 431, 748, 1323
MvnI	CGCG	4 cut(s)	185, 421, 423, 774
MwoI	GCNNNNNNNGC	7 cut(s)	258, 360, 420, 431, 500, 512, 848
NciI	CCSGG	5 cut(s)	360, 1095, 1096, 1346, 1548
NcoI	CCATGG	1 cut(s)	168
NdeII	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
NlaIV	GGNNCC	8 cut(s)	174, 216, 770, 912, 945, 1060, 1320, 1342
NmeAIII	GCCGAG	3 cut(s)	287, 300, 590
NmuCI	GTSAC	6 cut(s)	566, 872, 880, 1177, 1259, 1301
NspI	RCATGY	3 cut(s)	358, 412, 1546
PaeI	GCATGC	1 cut(s)	358
PaeR7I	CTCGAG	2 cut(s)	521, 1357
PasI	CCCWGGG	1 cut(s)	747
PauI	GCGCGC	1 cut(s)	421
PceI	AGGCCT	3 cut(s)	434, 698, 1222
PciI	ACATGT	1 cut(s)	408
PcsI	WCGNNNNNNNCGW	5 cut(s)	234, 381, 390, 399, 617
PctI	GAATGC	1 cut(s)	1294
PdmI	GAANNNNTTC	1 cut(s)	1401
PfeI	GAWTC	2 cut(s)	267, 1335
PflFI	GACNNNGTC	2 cut(s)	878, 1001
PflMI	CCANNNNNTGG	1 cut(s)	753
PleI	GAGTC	1 cut(s)	642
PpsI	GAGTC	1 cut(s)	642
PpuMI	RGGWCCY	1 cut(s)	911
PscI	ACATGT	1 cut(s)	408
Psp124BI	GAGCTC	1 cut(s)	276
Psp1406I	AACGTT	1 cut(s)	468
Psp5II	RGGWCCY	1 cut(s)	911
Psp6I	CCWGG	4 cut(s)	201, 429, 746, 1321
PspEI	GGTNACC	1 cut(s)	566
PspGI	CCWGG	4 cut(s)	201, 429, 746, 1321
PspN4I	GGNNCC	8 cut(s)	174, 216, 770, 912, 945, 1060, 1320, 1342
PspPI	GGNCC	8 cut(s)	214, 316, 550, 911, 944, 964, 1037, 1410
PspPPI	RGGWCCY	1 cut(s)	911
PspXI	VCTCGAGB	1 cut(s)	521
PstNI	CAGNNNCTG	1 cut(s)	753
PsuI	RGATCY	3 cut(s)	222, 606, 1213
PsyI	GACNNNGTC	2 cut(s)	878, 1001
PteI	GCGCGC	1 cut(s)	421
RsaI	GTAC	5 cut(s)	344, 584, 743, 869, 1574
RsaNI	GTAC	5 cut(s)	343, 583, 742, 868, 1573
RseI	CAYNNNNRTG	1 cut(s)	1277
SacI	GAGCTC	1 cut(s)	276
SaqAI	TTAA	2 cut(s)	1077, 1582
Sau3AI	GATC	6 cut(s)	158, 222, 399, 606, 927, 1213
Sau96I	GGNCC	8 cut(s)	214, 316, 550, 911, 944, 964, 1037, 1410
ScaI	AGTACT	2 cut(s)	344, 869
SchI	GAGTC	1 cut(s)	643
ScrFI	CCNGG	9 cut(s)	203, 360, 431, 748, 1095, 1096, 1323, 1346, 1548
SduI	GDGCHC	5 cut(s)	276, 528, 799, 1063, 1345
SfaNI	GCATC	1 cut(s)	546
SfcI	CTRYAG	1 cut(s)	934
Sfr274I	CTCGAG	2 cut(s)	521, 1357
SinI	GGWCC	5 cut(s)	214, 550, 911, 944, 1037
SlaI	CTCGAG	2 cut(s)	521, 1357
SmaI	CCCGGG	1 cut(s)	1096
SmiMI	CAYNNNNRTG	1 cut(s)	1277
SmlI	CTYRAG	6 cut(s)	521, 904, 1076, 1357, 1457, 1505
SmoI	CTYRAG	6 cut(s)	521, 904, 1076, 1357, 1457, 1505
SphI	GCATGC	1 cut(s)	358
Sse9I	AATT	4 cut(s)	652, 716, 723, 1397
SseBI	AGGCCT	3 cut(s)	434, 698, 1222
SspMI	CTAG	2 cut(s)	915, 1064
SstI	GAGCTC	1 cut(s)	276
StuI	AGGCCT	3 cut(s)	434, 698, 1222
StyD4I	CCNGG	9 cut(s)	201, 358, 429, 746, 1093, 1094, 1321, 1344, 1546
StyI	CCWWGG	6 cut(s)	168, 699, 914, 1141, 1314, 1557
TaaI	ACNGT	2 cut(s)	872, 1279
TaiI	ACGT	1 cut(s)	471
TasI	AATT	4 cut(s)	652, 716, 723, 1397
TatI	WGTACW	3 cut(s)	342, 867, 1572
TauI	GCSGC	7 cut(s)	255, 417, 452, 506, 732, 735, 831
TfiI	GAWTC	2 cut(s)	267, 1335
Tru1I	TTAA	2 cut(s)	1077, 1582
Tru9I	TTAA	2 cut(s)	1077, 1582
TscAI	CASTG	2 cut(s)	742, 1315
TseFI	GTSAC	6 cut(s)	566, 872, 880, 1177, 1259, 1301
TseI	GCWGC	3 cut(s)	324, 839, 851
Tsp45I	GTSAC	6 cut(s)	566, 872, 880, 1177, 1259, 1301
TspDTI	ATGAA	3 cut(s)	379, 658, 1583
TspGWI	ACGGA	3 cut(s)	116, 151, 390
TspMI	CCCGGG	1 cut(s)	1094
TspRI	CASTG	2 cut(s)	742, 1315
Tth111I	GACNNNGTC	2 cut(s)	878, 1001
Van91I	CCANNNNNTGG	1 cut(s)	753
Vha464I	CTTAAG	1 cut(s)	1076
VpaK11BI	GGWCC	5 cut(s)	214, 550, 911, 944, 1037
XapI	RAATTY	1 cut(s)	1397
XceI	RCATGY	3 cut(s)	358, 412, 1546
XcmI	CCANNNNNNNNNTGG	2 cut(s)	183, 1442
XhoI	CTCGAG	2 cut(s)	521, 1357
XmaI	CCCGGG	1 cut(s)	1094
XmaJI	CCTAGG	1 cut(s)	914
XmnI	GAANNNNTTC	1 cut(s)	1401
XspI	CTAG	2 cut(s)	915, 1064
ZrmI	AGTACT	2 cut(s)	344, 869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.