MD05G1252900.v1.1

Cysteine-rich TM module stress tolerance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0016286
Physical Location & Seq
Reverse (-)
38424263 .. 38424991
729 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1252900.v1.1.491

Sequence Viewer

Length: 363 bp
ATGAGTCACTACAACCATGATCATCATCAGGCTCCGCCCACTTCATACCTTCCTCCACCGGCCGGTGCATATCAAGGGCATCTGCCGCCGTCGTACCCTCCACCACCAGAAGTTCAGCAAGGATACAGTACTATAACTCAAGGTGGTCCGTATGTTGTTGTTGCACCACCAGTTAATTACCCTATGAAAAGTGATGGACCTCCAGCTGGATACCCCCAAGAGACAGCTCCCTATCAGCAGCAGCAACAGCCACCAAAATTTCATCACCAACAGAGAGGTGGTGGATTTTGGGCGGGATGTTGTTCTGCCATGTTTTGCTGCTGCCTCTGCGACCTGTGCTGCTTGCCCTGCGCAGTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.01

Weight (kDa)

6.33

Isoelectric Point (pI)

70.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 78 - 114 5.6e-07 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016286)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12190
malus_domestica MD05G1252900.v1.1 MD10G1234000.v1.1
prunus_persica Prupe.4G110700_v2.0.a1 Prupe.4G110900_v2.0.a1 Prupe.4G111000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0019971
rosa_laevigata RLG00000032490
rosa_roxburghii Rroxscaffold_1G00058220
rosa_rugosa Rorug05G0055900
rosa_samantha Rh5AG146000 Rh5BG145300 Rh5CG157000 Rh5DG144800
rosa_wichuraiana Rw5G012890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 352
AciI CCGC 3 cut(s) 35, 86, 293
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 1 cut(s) 257
AfaI GTAC 2 cut(s) 95, 130
AfiI CCNNNNNNNGG 2 cut(s) 62, 206
AluBI AGCT 2 cut(s) 206, 227
AluI AGCT 2 cut(s) 206, 227
Alw26I GTCTC 1 cut(s) 215
AoxI GGCC 1 cut(s) 60
ApeKI GCWGC 5 cut(s) 238, 241, 318, 321, 339
ApoI RAATTY 1 cut(s) 257
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 2 cut(s) 146, 197
AsuHPI GGTGA 1 cut(s) 257
AvaII GGWCC 2 cut(s) 146, 197
BbvI GCAGC 5 cut(s) 250, 253, 305, 308, 326
BccI CCATC 1 cut(s) 188
BceAI ACGGC 1 cut(s) 73
BciVI GTATCC 2 cut(s) 116, 203
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 215
BfuI GTATCC 2 cut(s) 116, 203
BisI GCNGC 6 cut(s) 86, 239, 242, 319, 322, 340
BlsI GCNGC 6 cut(s) 87, 240, 243, 320, 323, 341
BmcAI AGTACT 1 cut(s) 130
Bme18I GGWCC 2 cut(s) 146, 197
BmgT120I GGNCC 2 cut(s) 146, 197
BmiI GGNNCC 1 cut(s) 33
BmsI GCATC 1 cut(s) 88
BpmI CTGGAG 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 24
Bsc4I CCNNNNNNNGG 2 cut(s) 62, 206
Bse118I RCCGGY 2 cut(s) 58, 62
Bse1I ACTGG 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 24
BseGI GGATG 1 cut(s) 302
BseJI GATNNNNATC 1 cut(s) 24
BseLI CCNNNNNNNGG 2 cut(s) 62, 206
BseNI ACTGG 1 cut(s) 170
BseX3I CGGCCG 1 cut(s) 60
BseXI GCAGC 5 cut(s) 250, 253, 305, 308, 326
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 1 cut(s) 62
BsiEI CGRYCG 1 cut(s) 63
BsiSI CCGG 2 cut(s) 59, 63
BslI CCNNNNNNNGG 2 cut(s) 62, 206
BsmAI GTCTC 1 cut(s) 215
BsnI GGCC 1 cut(s) 62
Bsp143I GATC 1 cut(s) 19
BspACI CCGC 3 cut(s) 35, 86, 293
BspANI GGCC 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 33
BsrFI RCCGGY 2 cut(s) 58, 62
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 2 cut(s) 58, 62
BssMI GATC 1 cut(s) 19
Bst4CI ACNGT 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 344
BstF5I GGATG 1 cut(s) 302
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 1 cut(s) 22
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 1 cut(s) 19
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 5 cut(s) 85, 247, 327, 336, 348
BstV1I GCAGC 5 cut(s) 250, 253, 305, 308, 326
BstZI CGGCCG 1 cut(s) 60
BsuI GTATCC 2 cut(s) 116, 203
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 344
CfoI GCGC 1 cut(s) 353
Cfr10I RCCGGY 2 cut(s) 58, 62
Cfr13I GGNCC 2 cut(s) 146, 197
Csp6I GTAC 2 cut(s) 94, 129
CviAII CATG 2 cut(s) 17, 310
CviJI RGCY 5 cut(s) 32, 62, 206, 227, 250
CviKI_1 RGCY 5 cut(s) 32, 62, 206, 227, 250
CviQI GTAC 2 cut(s) 94, 129
DpnI GATC 1 cut(s) 21
DpnII GATC 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 60
EagI CGGCCG 1 cut(s) 60
EciI GGCGGA 1 cut(s) 24
EclXI CGGCCG 1 cut(s) 60
Eco47I GGWCC 2 cut(s) 146, 197
Eco52I CGGCCG 1 cut(s) 60
FaeI CATG 2 cut(s) 20, 313
FaiI YATR 7 cut(s) 18, 46, 70, 134, 153, 185, 311
FatI CATG 2 cut(s) 16, 309
FauI CCCGC 1 cut(s) 286
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 6 cut(s) 86, 239, 242, 319, 322, 340
FokI GGATG 1 cut(s) 309
Fsp4HI GCNGC 6 cut(s) 86, 239, 242, 319, 322, 340
FspI TGCGCA 1 cut(s) 352
GlaI GCGC 1 cut(s) 352
GluI GCNGC 6 cut(s) 86, 239, 242, 319, 322, 340
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 1 cut(s) 62
HapII CCGG 2 cut(s) 59, 63
HhaI GCGC 1 cut(s) 353
Hin1II CATG 2 cut(s) 20, 313
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HinfI GANTC 1 cut(s) 4
HpaII CCGG 2 cut(s) 59, 63
HphI GGTGA 1 cut(s) 257
Hpy99I CGWCG 1 cut(s) 94
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 1 cut(s) 128
HpyCH4V TGCA 2 cut(s) 68, 164
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 247, 327, 336, 348
Hsp92II CATG 2 cut(s) 20, 313
HspAI GCGC 1 cut(s) 351
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 1 cut(s) 19
LmnI GCTCC 2 cut(s) 37, 232
LpnPI CCDG 8 cut(s) 14, 72, 76, 120, 183, 192, 216, 347
Lsp1109I GCAGC 5 cut(s) 250, 253, 305, 308, 326
LweI GCATC 1 cut(s) 88
MaeIII GTNAC 1 cut(s) 5
MalI GATC 1 cut(s) 21
MboI GATC 1 cut(s) 19
MluCI AATT 2 cut(s) 175, 257
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 5 cut(s) 63, 108, 210, 269, 335
MseI TTAA 2 cut(s) 174, 361
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 2 cut(s) 59, 63
MwoI GCNNNNNNNGC 5 cut(s) 85, 247, 327, 336, 348
NdeII GATC 1 cut(s) 19
NlaIII CATG 2 cut(s) 20, 313
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 5
NsbI TGCGCA 1 cut(s) 352
PkrI GCNGC 6 cut(s) 87, 240, 243, 320, 323, 341
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 2 cut(s) 146, 197
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 2 cut(s) 95, 130
RsaNI GTAC 2 cut(s) 94, 129
SaqAI TTAA 2 cut(s) 174, 361
SatI GCNGC 6 cut(s) 86, 239, 242, 319, 322, 340
Sau3AI GATC 1 cut(s) 19
Sau96I GGNCC 2 cut(s) 146, 197
ScaI AGTACT 1 cut(s) 130
SchI GAGTC 1 cut(s) 13
SetI ASST 7 cut(s) 51, 145, 202, 208, 229, 280, 336
SfaNI GCATC 1 cut(s) 88
SinI GGWCC 2 cut(s) 146, 197
SmlI CTYRAG 1 cut(s) 138
SmoI CTYRAG 1 cut(s) 138
Sse9I AATT 2 cut(s) 175, 257
SsiI CCGC 3 cut(s) 35, 86, 293
TaaI ACNGT 1 cut(s) 128
TasI AATT 2 cut(s) 175, 257
TatI WGTACW 1 cut(s) 128
TauI GCSGC 1 cut(s) 88
Tru1I TTAA 2 cut(s) 174, 361
Tru9I TTAA 2 cut(s) 174, 361
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 5 cut(s) 238, 241, 318, 321, 339
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 3 cut(s) 33, 200, 251
TspGWI ACGGA 1 cut(s) 138
VpaK11BI GGWCC 2 cut(s) 146, 197
XapI RAATTY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
ZrmI AGTACT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.