Rh5BG145300

Cysteine-rich TM module stress tolerance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016286
Physical Location & Seq
Forward (+)
15050304 .. 15051680
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG145300.1

Sequence Viewer

Length: 336 bp
ATGAGTCACTACAACCAGCATCACCAGGCTGCAGGAGTATATCCACCACCACCAACCTCATACCCTCCACCGCCAGCTGGGGCATATCCTCCTCCAGGGCACGCTTATCCTCCGCCACCGGTTCAAGGATACAATCAAGGTCCTTATGTTGTTCCACCACCAGTTTCATACCCCATGAAAAATAATGGACCAGCTGAGGGATACCCCCAAGGTCCTCAACAATCAGCTCCCTATAGGCACAAGGGCAAAGGCGGCGGATTTTGGACCGGATGTTGTTCTGCCATGTTTTGCTGCTGCCTCTGCGATTTGTGCTGCTTGCCTTGCTCAATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

111

Amino Acids

11.83

Weight (kDa)

8.08

Isoelectric Point (pI)

66.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 67 - 105 3e-09 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016286)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g12190
malus_domestica MD05G1252900.v1.1 MD10G1234000.v1.1
prunus_persica Prupe.4G110700_v2.0.a1 Prupe.4G110900_v2.0.a1 Prupe.4G111000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0019971
rosa_laevigata RLG00000032490
rosa_roxburghii Rroxscaffold_1G00058220
rosa_rugosa Rorug05G0055900
rosa_samantha Rh5AG146000 Rh5BG145300 Rh5CG157000 Rh5DG144800
rosa_wichuraiana Rw5G012890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 71, 113, 252, 255
AfiI CCNNNNNNNGG 4 cut(s) 77, 95, 125, 197
AgeI ACCGGT 1 cut(s) 118
AgsI TTSAA 1 cut(s) 125
AjnI CCWGG 2 cut(s) 24, 94
AluBI AGCT 3 cut(s) 77, 194, 227
AluI AGCT 3 cut(s) 77, 194, 227
ApeKI GCWGC 4 cut(s) 29, 291, 294, 312
AsiGI ACCGGT 1 cut(s) 118
AspS9I GGNCC 4 cut(s) 140, 188, 212, 264
AsuHPI GGTGA 1 cut(s) 14
AvaII GGWCC 4 cut(s) 140, 188, 212, 264
BaeGI GKGCMC 1 cut(s) 102
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 4 cut(s) 16, 278, 281, 299
BciT130I CCWGG 2 cut(s) 26, 96
BciVI GTATCC 2 cut(s) 122, 194
BfmI CTRYAG 2 cut(s) 30, 232
BfuI GTATCC 2 cut(s) 122, 194
BisI GCNGC 5 cut(s) 30, 253, 292, 295, 313
BlsI GCNGC 5 cut(s) 31, 254, 293, 296, 314
Bme1390I CCNGG 2 cut(s) 26, 96
Bme18I GGWCC 4 cut(s) 140, 188, 212, 264
BmgT120I GGNCC 4 cut(s) 140, 188, 212, 264
BmrFI CCNGG 2 cut(s) 26, 96
BmsI GCATC 1 cut(s) 28
BpmI CTGGAG 1 cut(s) 78
Bpu10I CCTNAGC 1 cut(s) 195
BsaJI CCNNGG 2 cut(s) 95, 208
BsaWI WCCGGW 2 cut(s) 118, 266
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 95, 125, 197
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 1 cut(s) 161
BseBI CCWGG 2 cut(s) 26, 96
BseDI CCNNGG 2 cut(s) 95, 208
BseGI GGATG 1 cut(s) 275
BseLI CCNNNNNNNGG 4 cut(s) 77, 95, 125, 197
BseMII CTCAG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 161
BseRI GAGGAG 1 cut(s) 81
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 4 cut(s) 16, 278, 281, 299
BseYI CCCAGC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 118
BsiSI CCGG 2 cut(s) 119, 267
BslI CCNNNNNNNGG 4 cut(s) 77, 95, 125, 197
Bsp1286I GDGCHC 1 cut(s) 102
BspACI CCGC 4 cut(s) 71, 113, 252, 255
BspCNI CTCAG 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 34
BsrFI RCCGGY 1 cut(s) 118
BsrI ACTGG 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 95, 208
BssT1I CCWWGG 1 cut(s) 208
Bst2UI CCWGG 2 cut(s) 26, 96
BstC8I GCNNGC 3 cut(s) 75, 102, 317
BstDEI CTNAG 1 cut(s) 195
BstENI CCTNNNNNAGG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 275
BstMWI GCNNNNNNNGC 4 cut(s) 252, 300, 309, 321
BstNI CCWGG 2 cut(s) 26, 96
BstSCI CCNGG 2 cut(s) 24, 94
BstSFI CTRYAG 2 cut(s) 30, 232
BstSLI GKGCMC 1 cut(s) 102
BstV1I GCAGC 4 cut(s) 16, 278, 281, 299
BsuI GTATCC 2 cut(s) 122, 194
BtsCI GGATG 1 cut(s) 275
Cac8I GCNNGC 3 cut(s) 75, 102, 317
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 4 cut(s) 140, 188, 212, 264
CspAI ACCGGT 1 cut(s) 118
CviAII CATG 2 cut(s) 175, 283
CviJI RGCY 4 cut(s) 29, 77, 194, 227
CviKI_1 RGCY 4 cut(s) 29, 77, 194, 227
DdeI CTNAG 1 cut(s) 195
EciI GGCGGA 2 cut(s) 102, 270
Eco130I CCWWGG 1 cut(s) 208
Eco47I GGWCC 4 cut(s) 140, 188, 212, 264
EcoNI CCTNNNNNAGG 1 cut(s) 93
EcoO109I RGGNCCY 2 cut(s) 140, 212
EcoRII CCWGG 2 cut(s) 24, 94
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 2 cut(s) 178, 286
FaiI YATR 8 cut(s) 40, 61, 85, 147, 169, 176, 234, 284
FatI CATG 2 cut(s) 174, 282
Fnu4HI GCNGC 5 cut(s) 30, 253, 292, 295, 313
FokI GGATG 1 cut(s) 282
Fsp4HI GCNGC 5 cut(s) 30, 253, 292, 295, 313
GluI GCNGC 5 cut(s) 30, 253, 292, 295, 313
GsaI CCCAGC 1 cut(s) 81
GsuI CTGGAG 1 cut(s) 78
HapII CCGG 2 cut(s) 119, 267
Hin1II CATG 2 cut(s) 178, 286
HinfI GANTC 1 cut(s) 4
HpaII CCGG 2 cut(s) 119, 267
HphI GGTGA 1 cut(s) 14
HpyCH4V TGCA 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 4 cut(s) 252, 300, 309, 321
HpyF3I CTNAG 1 cut(s) 195
Hsp92II CATG 2 cut(s) 178, 286
LmnI GCTCC 1 cut(s) 232
Lsp1109I GCAGC 4 cut(s) 16, 278, 281, 299
LweI GCATC 1 cut(s) 28
MaeIII GTNAC 1 cut(s) 5
MhlI GDGCHC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 13
MnlI CCTC 8 cut(s) 67, 75, 99, 102, 120, 190, 225, 308
MseI TTAA 1 cut(s) 334
MspA1I CMGCKG 2 cut(s) 77, 194
MspI CCGG 2 cut(s) 119, 267
MspR9I CCNGG 2 cut(s) 26, 96
MvaI CCWGG 2 cut(s) 26, 96
MwoI GCNNNNNNNGC 4 cut(s) 252, 300, 309, 321
NlaIII CATG 2 cut(s) 178, 286
NmuCI GTSAC 1 cut(s) 5
PinAI ACCGGT 1 cut(s) 118
PkrI GCNGC 5 cut(s) 31, 254, 293, 296, 314
PleI GAGTC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 12
PpuMI RGGWCCY 2 cut(s) 140, 212
Psp5II RGGWCCY 2 cut(s) 140, 212
Psp6I CCWGG 2 cut(s) 24, 94
PspFI CCCAGC 1 cut(s) 77
PspGI CCWGG 2 cut(s) 24, 94
PspPI GGNCC 4 cut(s) 140, 188, 212, 264
PspPPI RGGWCCY 2 cut(s) 140, 212
PstI CTGCAG 1 cut(s) 34
PvuII CAGCTG 2 cut(s) 77, 194
SaqAI TTAA 1 cut(s) 334
SatI GCNGC 5 cut(s) 30, 253, 292, 295, 313
Sau96I GGNCC 4 cut(s) 140, 188, 212, 264
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 26, 96
SduI GDGCHC 1 cut(s) 102
SetI ASST 6 cut(s) 59, 79, 142, 196, 214, 229
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 2 cut(s) 30, 232
SinI GGWCC 4 cut(s) 140, 188, 212, 264
SsiI CCGC 4 cut(s) 71, 113, 252, 255
StyD4I CCNGG 2 cut(s) 24, 94
StyI CCWWGG 1 cut(s) 208
TauI GCSGC 1 cut(s) 255
Tru1I TTAA 1 cut(s) 334
Tru9I TTAA 1 cut(s) 334
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 4 cut(s) 29, 291, 294, 312
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 2 cut(s) 156, 191
VpaK11BI GGWCC 4 cut(s) 140, 188, 212, 264
XagI CCTNNNNNAGG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.