Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004985

Physical Location & Seq

Genomic Coordinates Reverse (-)

43252854 .. 43257982

Gene Structure 5129 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1299200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGGGGATGGATGGAACCCTCTCGGGTTCCGGTTGCTATCTCTCCTGGTTTTGATTCTTGTTGTGAGTTTTCGGGGCTGTTGGTGTCTCAATGATGAAGGATTGATTCTGTTGTCATTCCGTGAAAATATCAAGTTCGATCCTTTTGGTGCTTTGGAGAATTGGAGTCCTAACGATGGCGATCCTTGCCTGTGGAATGGTGTTCATTGTGTGGACGGTAAAGTGCATATGCTGAATCTGACGGATTTTTCTCTGGAAGGGACATTGGTACCTGAACTTTGGAAACTTAATCACTTACGATCTCTTGTGTTATACAAGAATCGCTTATCTGGTGTAATTCCTAAAGAGATTGGGGGGCTCACAAGGTTGGAGCTACTGGATTTGAGGAACAATAACTTGAATGGGATGATTCCAGTTGAGTTAGGCAGTATGCTGTCACTGAAATGCTTGTTGCTTTGTGACAATAGATTCGAAGGCACCATTCCTCTAGAGCTCACTCGTCTCAGCTCGCTCTGCGAATTGCAATTTGATGATTACCTTTCATCTAGTGAAACCACTGGATTTGGCTGTGTGAACAGAAAGTTTGGCCATTGCATCTGGCAGAGCAATTTAGAACATGTGAATACTGTGGAATCCTTGGTATTATTTCTTAAAGGAGCTTTTAAGAACTATCTCAACTACTTGTCACTGCCTCGGTTCAGTTACAGAAAGGATCCTCTGCCAATTGATGGAGATAATTGCTGTGATAGTTTATCTAGTTGGTTTGAGTCGCACATGGCCTTTGCTCGCCGTAGACTACTTGCACAAGCCAGTAACCTACCAGCTGTACCCGTTACTGATACGGAACCAACTGGACAGGTCATTGCTCTTCCAACTACCCGAAGTAGTGGTGCTTTCCCAGCCGTGCCAAAAGATAAAAAGAAAAATTCTCCTCCGCCTGCACCTGCACCGCAGCCTTCGCCTGAACCTCATGAGGCTTCAAAACATGATGGACAATCTTCCCAAAAATTAATTGGCAATATGTGGAAGTATCTCATTATTGGTTTCAGTGTGCTTATCTTGCTCCTTGTTCTTATGGCCATGGTCTTCATGTGTCGAAGCCAAGTGGCTAAAACTGTAGGTCCCTGGAAGACTGGATTGAGTGGACAGTTGCAGAAAGCATTTATATCAGGAGTTCCCAAGTTGAACCGATCAGAACTGGAAACCGCATGTGAAGATTTCAGTAACATTATTAGCACACTTGAAGGTTGTATTGTGTACAAGGGAACACTGTCCAGTGGAGTTGAGATTGCTGTTGCATCAACTACAATATCGTCTTTGAAAGACTGGTCCAAGCTTGCAGAGAATTGTTACCGGAAAAAGATTGACGTACTGTCACGCGTGAATCATAAGAACTTTGTCAATCTTATTGGCTACTGTGAGGAGGATGAGCCTTTTACTAGGATGATGGTGTTTGAGTATGCTCCAAATGGAAATCTCTTTGAGCATCTGCATATTGAAGAAATGGAACATCTGGATTGGAATTCGAGGATTAGGATCGTTATGGGAACTGCTTATTGTCTTCAGTACATGCACCATGAGCTAAATCCACCTGTGTCACATCCTAACCTGACCTCGGCTTCTATCTTTTTGACGGATGATTACGCTGCTAAGATTGCGGAGATCTGTTTCTGGGCAGAATCAATACGAAAGCCTAAGAATTCCGGTGATGATGATAAGGAGCATTCGGTATTGCCACCCCTTGCTGATCCAGAAACAAACGTCTACAGTTTCGGAGTAATGCTGCTTGAAATCATCACCGGGAAGCTCCAAAACTCAGAAGAACATGGGTCCCTTCTATACTGGGCCTCTGCATATCTGAACGAGAATCGAAGCGACATGGTGGATCCAACCCTCAAGTCGTTCAAAAACGAAGAGCTGGATGTCCTGTGCGAGGTCATCAAAGATTGCATCCAAGAAGATCCAAGGCAGAGACCAACAATGAAGGATGTGACTAACAAACTGAGGGAAGTGATCCCCATCACTCCCAACCAAGCAGTCCCCAGACTCTCTCCCCTCTGGTGGGCGGAACTCGAGATCTTATCGGTCGAGGCGACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0021700 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042995 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071695 GO:0071704 GO:0071944 GO:0090406 GO:0090558 GO:0090627 GO:0099402 GO:0120025 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

700

Amino Acids

78.56

Weight (kDa)

5.5

Isoelectric Point (pI)

41.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 70	3.7e-11	Leucine rich repeat N-terminal domain
LRR_14	PF23598	77 - 182	9e-10	Leucine-rich repeat region
LRR_8	PF13855	98 - 156	3.8e-06	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	419 - 668	3.3e-19	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	420 - 666	4.3e-14	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18640 AT5G45840 AT5G45840
fragaria_vesca	FvH4_3g07660 FvH4_3g07660 FvH4_3g07660 FvH4_3g07660 FvH4_3g07660 FvH4_3g07660
malus_domestica	MD05G1299200.v1.1 MD10G1278700.v1.1
prunus_persica	Prupe.4G062600_v2.0.a1 Prupe.4G062600_v2.0.a1 Prupe.4G062600_v2.0.a1 Prupe.4G062600_v2.0.a1
pyrus_communis	pycom05g27710 pycom10g23270
rosa_chinensis	RchiOBHm_Chr5g0011021
rosa_laevigata	RLG00000031803
rosa_roxburghii	Rroxscaffold_1G00065380
rosa_rugosa	Rorug04G0454500 Rorug04G0454600 Rorug04G0454600 Rorug04G0454600
rosa_samantha	Rh5AG086800 Rh5BG081400 Rh5CG094200 Rh5DG081300
rosa_wichuraiana	Rw0G000120 Rw0G017830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	952
Acc36I	ACCTGC	1 cut(s)	952
Acc65I	GGTACC	1 cut(s)	268
AccB1I	GGYRCC	2 cut(s)	268, 476
AccB7I	CCANNNNNTGG	1 cut(s)	728
AccI	GTMKAC	2 cut(s)	793, 1764
AccII	CGCG	1 cut(s)	1380
AciI	CCGC	5 cut(s)	935, 950, 1206, 1658, 2066
AcoI	YGGCCR	2 cut(s)	586, 1077
AcsI	RAATTY	3 cut(s)	925, 1522, 1699
AcuI	CTGAAG	1 cut(s)	1547
AfaI	GTAC	5 cut(s)	270, 828, 1259, 1371, 1568
AfiI	CCNNNNNNNGG	6 cut(s)	176, 728, 1615, 1933, 2061, 2062
AflIII	ACRYGT	2 cut(s)	616, 1378
AgsI	TTSAA	8 cut(s)	400, 981, 1186, 1244, 1321, 1499, 1790, 1906
AhdI	GACNNNNNGTC	1 cut(s)	1372
AjnI	CCWGG	2 cut(s)	45, 1124
AluBI	AGCT	9 cut(s)	373, 493, 507, 659, 824, 1336, 1582, 1807, 1918
AluI	AGCT	9 cut(s)	373, 493, 507, 659, 824, 1336, 1582, 1807, 1918
Alw21I	GWGCWC	1 cut(s)	495
Alw26I	GTCTC	3 cut(s)	92, 506, 1966
Ama87I	CYCGRG	2 cut(s)	23, 2072
AoxI	GGCC	4 cut(s)	586, 777, 1077, 1845
ApeKI	GCWGC	3 cut(s)	952, 1646, 1783
ApoI	RAATTY	3 cut(s)	925, 1522, 1699
ArsI	GACNNNNNNTTYG	2 cut(s)	452, 484
AseI	ATTAAT	1 cut(s)	1010
Asp718I	GGTACC	1 cut(s)	268
AspS9I	GGNCC	4 cut(s)	1121, 1329, 1830, 1845
AsuC2I	CCSGG	1 cut(s)	1801
AsuHPI	GGTGA	2 cut(s)	1718, 1789
AsuII	TTCGAA	1 cut(s)	471
AvaI	CYCGRG	2 cut(s)	23, 2072
AvaII	GGWCC	3 cut(s)	1121, 1329, 1830
BaeI	ACNNNNGTAYC	2 cut(s)	252, 285
BalI	TGGCCA	2 cut(s)	588, 1079
BamHI	GGATCC	2 cut(s)	712, 1885
BanI	GGYRCC	2 cut(s)	268, 476
BanII	GRGCYC	2 cut(s)	360, 495
BbsI	GAAGAC	3 cut(s)	1078, 1136, 1553
Bbv12I	GWGCWC	1 cut(s)	495
BbvI	GCAGC	3 cut(s)	964, 1633, 1770
BccI	CCATC	7 cut(s)	2, 6, 170, 722, 983, 1441, 2027
BceAI	ACGGC	2 cut(s)	774, 887
BciT130I	CCWGG	2 cut(s)	47, 1126
BcnI	CCSGG	1 cut(s)	1801
BcoDI	GTCTC	3 cut(s)	92, 506, 1966
BfaI	CTAG	4 cut(s)	488, 546, 756, 1440
BfmI	CTRYAG	2 cut(s)	1116, 1765
BfuAI	ACCTGC	1 cut(s)	952
BglII	AGATCT	2 cut(s)	1662, 2076
BisI	GCNGC	3 cut(s)	953, 1647, 1784
BlsI	GCNGC	3 cut(s)	954, 1648, 1785
Bme1390I	CCNGG	3 cut(s)	47, 1126, 1801
Bme18I	GGWCC	3 cut(s)	1121, 1329, 1830
BmeRI	GACNNNNNGTC	1 cut(s)	1372
BmeT110I	CYCGRG	2 cut(s)	23, 2072
BmgT120I	GGNCC	4 cut(s)	1121, 1329, 1830, 1845
BmrFI	CCNGG	3 cut(s)	47, 1126, 1801
BmrI	ACTGGG	1 cut(s)	1852
BmsI	GCATC	4 cut(s)	603, 1307, 1495, 1959
BmuI	ACTGGG	1 cut(s)	1852
BpiI	GAAGAC	3 cut(s)	1078, 1136, 1553
Bpu14I	TTCGAA	1 cut(s)	471
BpuEI	CTTGAG	1 cut(s)	1880
BpuMI	CCSGG	1 cut(s)	1801
BsaBI	GATNNNNATC	3 cut(s)	180, 1535, 2018
BsaI	GGTCTC	1 cut(s)	1966
BsaJI	CCNNGG	6 cut(s)	636, 692, 1080, 1124, 1614, 1964
BsaWI	WCCGGW	3 cut(s)	30, 1353, 1703
Bsc4I	CCNNNNNNNGG	6 cut(s)	176, 728, 1615, 1933, 2061, 2062
Bse3DI	GCAATG	2 cut(s)	589, 861
Bse8I	GATNNNNATC	3 cut(s)	180, 1535, 2018
BseBI	CCWGG	2 cut(s)	47, 1126
BseDI	CCNNGG	6 cut(s)	636, 692, 1080, 1124, 1614, 1964
BseJI	GATNNNNATC	3 cut(s)	180, 1535, 2018
BseLI	CCNNNNNNNGG	6 cut(s)	176, 728, 1615, 1933, 2061, 2062
BseMI	GCAATG	2 cut(s)	589, 861
BseMII	CTCAG	3 cut(s)	517, 1830, 1994
BseRI	GAGGAG	2 cut(s)	921, 1436
BseXI	GCAGC	3 cut(s)	964, 1633, 1770
BseYI	CCCAGC	1 cut(s)	898
BsgI	GTGCAG	2 cut(s)	924, 930
Bsh1236I	CGCG	1 cut(s)	1380
Bsh1285I	CGRYCG	1 cut(s)	2088
BshFI	GGCC	4 cut(s)	588, 779, 1079, 1847
BshNI	GGYRCC	2 cut(s)	268, 476
BsiEI	CGRYCG	1 cut(s)	2088
BsiHKAI	GWGCWC	1 cut(s)	495
BsiHKCI	CYCGRG	2 cut(s)	23, 2072
BsiSI	CCGG	4 cut(s)	31, 1354, 1704, 1800
BslFI	GGGAC	4 cut(s)	274, 1107, 1816, 2024
BslI	CCNNNNNNNGG	6 cut(s)	176, 728, 1615, 1933, 2061, 2062
BsmAI	GTCTC	3 cut(s)	92, 506, 1966
BsmBI	CGTCTC	1 cut(s)	506
BsmFI	GGGAC	4 cut(s)	274, 1107, 1816, 2024
BsmI	GAATGC	1 cut(s)	1723
BsnI	GGCC	4 cut(s)	588, 779, 1079, 1847
Bso31I	GGTCTC	1 cut(s)	1966
BsoBI	CYCGRG	2 cut(s)	23, 2072
Bsp119I	TTCGAA	1 cut(s)	471
Bsp1286I	GDGCHC	2 cut(s)	360, 495
Bsp1407I	TGTACA	1 cut(s)	1257
Bsp19I	CCATGG	1 cut(s)	1080
BspACI	CCGC	5 cut(s)	935, 950, 1206, 1658, 2066
BspANI	GGCC	4 cut(s)	588, 779, 1079, 1847
BspCNI	CTCAG	3 cut(s)	516, 1829, 1995
BspFNI	CGCG	1 cut(s)	1380
BspHI	TCATGA	1 cut(s)	970
BspMI	ACCTGC	1 cut(s)	952
BspQI	GCTCTTC	2 cut(s)	873, 1908
BspT104I	TTCGAA	1 cut(s)	471
BspT107I	GGYRCC	2 cut(s)	268, 476
BspTNI	GGTCTC	1 cut(s)	1966
BsrDI	GCAATG	2 cut(s)	589, 861
BsrGI	TGTACA	1 cut(s)	1257
BssECI	CCNNGG	6 cut(s)	636, 692, 1080, 1124, 1614, 1964
BssT1I	CCWWGG	3 cut(s)	636, 1080, 1964
Bst2UI	CCWGG	2 cut(s)	47, 1126
Bst4CI	ACNGT	8 cut(s)	218, 628, 1117, 1149, 1272, 1374, 1418, 1769
Bst6I	CTCTTC	2 cut(s)	873, 1908
BstAUI	TGTACA	1 cut(s)	1257
BstBI	TTCGAA	1 cut(s)	471
BstC8I	GCNNGC	4 cut(s)	509, 787, 939, 1338
BstDEI	CTNAG	5 cut(s)	503, 1650, 1695, 1816, 2003
BstDSI	CCRYGG	1 cut(s)	1080
BstENI	CCTNNNNNAGG	1 cut(s)	1931
BstFNI	CGCG	1 cut(s)	1380
BstMAI	GTCTC	3 cut(s)	92, 506, 1966
BstMCI	CGRYCG	1 cut(s)	2088
BstMWI	GCNNNNNNNGC	7 cut(s)	186, 513, 899, 958, 1060, 1579, 1655
BstNI	CCWGG	2 cut(s)	47, 1126
BstNSI	RCATGY	3 cut(s)	620, 1212, 1573
BstSCI	CCNGG	3 cut(s)	45, 1124, 1799
BstSFI	CTRYAG	2 cut(s)	1116, 1765
BstUI	CGCG	1 cut(s)	1380
BstV1I	GCAGC	3 cut(s)	964, 1633, 1770
BstV2I	GAAGAC	3 cut(s)	1078, 1136, 1553
BstX2I	RGATCY	5 cut(s)	712, 1662, 1885, 1960, 2076
BstYI	RGATCY	5 cut(s)	712, 1662, 1885, 1960, 2076
BsuRI	GGCC	4 cut(s)	588, 779, 1079, 1847
BtgI	CCRYGG	1 cut(s)	1080
BtsI	GCAGTG	1 cut(s)	686
BtsIMutI	CAGTG	6 cut(s)	437, 555, 686, 1054, 1268, 1282
BveI	ACCTGC	1 cut(s)	952
Cac8I	GCNNGC	4 cut(s)	509, 787, 939, 1338
CciI	TCATGA	1 cut(s)	970
Cfr13I	GGNCC	4 cut(s)	1121, 1329, 1830, 1845
Csp6I	GTAC	5 cut(s)	269, 827, 1258, 1370, 1567
CspCI	CAANNNNNGTGG	2 cut(s)	544, 579
CviQI	GTAC	5 cut(s)	269, 827, 1258, 1370, 1567
DdeI	CTNAG	5 cut(s)	503, 1650, 1695, 1816, 2003
DriI	GACNNNNNGTC	1 cut(s)	1372
EaeI	YGGCCR	2 cut(s)	586, 1077
Eam1104I	CTCTTC	2 cut(s)	873, 1908
Eam1105I	GACNNNNNGTC	1 cut(s)	1372
EarI	CTCTTC	2 cut(s)	873, 1908
EciI	GGCGGA	2 cut(s)	924, 2081
Ecl136II	GAGCTC	1 cut(s)	493
Eco130I	CCWWGG	3 cut(s)	636, 1080, 1964
Eco24I	GRGCYC	2 cut(s)	360, 495
Eco31I	GGTCTC	1 cut(s)	1966
Eco47I	GGWCC	3 cut(s)	1121, 1329, 1830
Eco53kI	GAGCTC	1 cut(s)	493
Eco57I	CTGAAG	1 cut(s)	1547
Eco88I	CYCGRG	2 cut(s)	23, 2072
EcoICRI	GAGCTC	1 cut(s)	493
EcoNI	CCTNNNNNAGG	1 cut(s)	1931
EcoO109I	RGGNCCY	2 cut(s)	1121, 1830
EcoRI	GAATTC	2 cut(s)	1522, 1699
EcoRII	CCWGG	2 cut(s)	45, 1124
EcoT14I	CCWWGG	3 cut(s)	636, 1080, 1964
EcoT38I	GRGCYC	2 cut(s)	360, 495
ErhI	CCWWGG	3 cut(s)	636, 1080, 1964
Esp3I	CGTCTC	1 cut(s)	506
FalI	AAGNNNNNCTT	2 cut(s)	308, 340
FaqI	GGGAC	4 cut(s)	274, 1107, 1816, 2024
FauNDI	CATATG	1 cut(s)	228
FblI	GTMKAC	2 cut(s)	793, 1764
Fnu4HI	GCNGC	3 cut(s)	953, 1647, 1784
FriOI	GRGCYC	2 cut(s)	360, 495
Fsp4HI	GCNGC	3 cut(s)	953, 1647, 1784
FspBI	CTAG	4 cut(s)	488, 546, 756, 1440
GluI	GCNGC	3 cut(s)	953, 1647, 1784
GsaI	CCCAGC	1 cut(s)	902
HaeIII	GGCC	4 cut(s)	588, 779, 1079, 1847
HapII	CCGG	4 cut(s)	31, 1354, 1704, 1800
HindIII	AAGCTT	1 cut(s)	1334
HpaII	CCGG	4 cut(s)	31, 1354, 1704, 1800
HphI	GGTGA	2 cut(s)	1718, 1789
Hpy166II	GTNNAC	6 cut(s)	214, 574, 794, 1145, 1258, 1765
Hpy188I	TCNGA	5 cut(s)	240, 1195, 1775, 1819, 1860
Hpy188III	TCNNGA	7 cut(s)	254, 488, 971, 1170, 1514, 1751, 2074
Hpy8I	GTNNAC	6 cut(s)	214, 574, 794, 1145, 1258, 1765
Hpy99I	CGWCG	1 cut(s)	2098
HpyAV	CCTTC	7 cut(s)	92, 251, 467, 966, 1238, 1844, 1978
HpyCH4III	ACNGT	8 cut(s)	218, 628, 1117, 1149, 1272, 1374, 1418, 1769
HpyCH4IV	ACGT	3 cut(s)	1368, 1761, 2096
HpyF10VI	GCNNNNNNNGC	7 cut(s)	186, 513, 899, 958, 1060, 1579, 1655
HpyF3I	CTNAG	5 cut(s)	503, 1650, 1695, 1816, 2003
HpySE526I	ACGT	3 cut(s)	1368, 1761, 2096
KflI	GGGWCCC	1 cut(s)	1830
KpnI	GGTACC	1 cut(s)	272
LguI	GCTCTTC	2 cut(s)	873, 1908
LmnI	GCTCC	6 cut(s)	370, 656, 1068, 1468, 1720, 1812
Lsp1109I	GCAGC	3 cut(s)	964, 1633, 1770
LweI	GCATC	4 cut(s)	603, 1307, 1495, 1959
MaeI	CTAG	4 cut(s)	488, 546, 756, 1440
MaeII	ACGT	3 cut(s)	1368, 1761, 2096
MfeI	CAATTG	1 cut(s)	723
MflI	RGATCY	5 cut(s)	712, 1662, 1885, 1960, 2076
MhlI	GDGCHC	2 cut(s)	360, 495
MlsI	TGGCCA	2 cut(s)	588, 1079
MluI	ACGCGT	1 cut(s)	1378
MluNI	TGGCCA	2 cut(s)	588, 1079
MlyI	GAGTC	3 cut(s)	175, 776, 2040
MmeI	TCCRAC	3 cut(s)	348, 896, 1913
Mox20I	TGGCCA	2 cut(s)	588, 1079
MscI	TGGCCA	2 cut(s)	588, 1079
MseI	TTAA	4 cut(s)	288, 651, 663, 1010
MslI	CAYNNNNRTG	1 cut(s)	442
Msp20I	TGGCCA	2 cut(s)	588, 1079
MspA1I	CMGCKG	1 cut(s)	824
MspI	CCGG	4 cut(s)	31, 1354, 1704, 1800
MspR9I	CCNGG	3 cut(s)	47, 1126, 1801
MunI	CAATTG	1 cut(s)	723
Mva1269I	GAATGC	1 cut(s)	1723
MvaI	CCWGG	2 cut(s)	47, 1126
MvnI	CGCG	1 cut(s)	1380
MwoI	GCNNNNNNNGC	7 cut(s)	186, 513, 899, 958, 1060, 1579, 1655
NciI	CCSGG	1 cut(s)	1801
NcoI	CCATGG	1 cut(s)	1080
NdeI	CATATG	1 cut(s)	228
NmeAIII	GCCGAG	1 cut(s)	1595
NmuCI	GTSAC	6 cut(s)	435, 458, 684, 1374, 1596, 1990
NspI	RCATGY	3 cut(s)	620, 1212, 1573
NspV	TTCGAA	1 cut(s)	471
PaeR7I	CTCGAG	1 cut(s)	2072
PagI	TCATGA	1 cut(s)	970
PaqCI	CACCTGC	1 cut(s)	952
PciI	ACATGT	1 cut(s)	616
PciSI	GCTCTTC	2 cut(s)	873, 1908
PcsI	WCGNNNNNNNCGW	1 cut(s)	2090
PctI	GAATGC	1 cut(s)	1723
PflMI	CCANNNNNTGG	1 cut(s)	728
PkrI	GCNGC	3 cut(s)	954, 1648, 1785
PleI	GAGTC	3 cut(s)	174, 775, 2040
PpsI	GAGTC	3 cut(s)	174, 775, 2040
PpuMI	RGGWCCY	2 cut(s)	1121, 1830
PscI	ACATGT	1 cut(s)	616
PshBI	ATTAAT	1 cut(s)	1010
Psp124BI	GAGCTC	1 cut(s)	495
Psp5II	RGGWCCY	2 cut(s)	1121, 1830
Psp6I	CCWGG	2 cut(s)	45, 1124
PspFI	CCCAGC	1 cut(s)	898
PspGI	CCWGG	2 cut(s)	45, 1124
PspPI	GGNCC	4 cut(s)	1121, 1329, 1830, 1845
PspPPI	RGGWCCY	2 cut(s)	1121, 1830
PsuI	RGATCY	5 cut(s)	712, 1662, 1885, 1960, 2076
PvuII	CAGCTG	1 cut(s)	824
RsaI	GTAC	5 cut(s)	270, 828, 1259, 1371, 1568
RsaNI	GTAC	5 cut(s)	269, 827, 1258, 1370, 1567
RseI	CAYNNNNRTG	1 cut(s)	442
SacI	GAGCTC	1 cut(s)	495
SapI	GCTCTTC	2 cut(s)	873, 1908
SaqAI	TTAA	4 cut(s)	288, 651, 663, 1010
SatI	GCNGC	3 cut(s)	953, 1647, 1784
Sau96I	GGNCC	4 cut(s)	1121, 1329, 1830, 1845
SchI	GAGTC	3 cut(s)	175, 776, 2040
ScrFI	CCNGG	3 cut(s)	47, 1126, 1801
SduI	GDGCHC	2 cut(s)	360, 495
SfaNI	GCATC	4 cut(s)	603, 1307, 1495, 1959
SfcI	CTRYAG	2 cut(s)	1116, 1765
Sfr274I	CTCGAG	1 cut(s)	2072
SfuI	TTCGAA	1 cut(s)	471
SinI	GGWCC	3 cut(s)	1121, 1329, 1830
SlaI	CTCGAG	1 cut(s)	2072
SmiMI	CAYNNNNRTG	1 cut(s)	442
SmlI	CTYRAG	2 cut(s)	1895, 2072
SmoI	CTYRAG	2 cut(s)	1895, 2072
SsiI	CCGC	5 cut(s)	935, 950, 1206, 1658, 2066
SspMI	CTAG	4 cut(s)	488, 546, 756, 1440
SstI	GAGCTC	1 cut(s)	495
StyD4I	CCNGG	3 cut(s)	45, 1124, 1799
StyI	CCWWGG	3 cut(s)	636, 1080, 1964
TaaI	ACNGT	8 cut(s)	218, 628, 1117, 1149, 1272, 1374, 1418, 1769
TaiI	ACGT	3 cut(s)	1371, 1764, 2099
TaqI	TCGA	7 cut(s)	138, 471, 1096, 1526, 1870, 2073, 2088
TaqII	GACCGA	1 cut(s)	2074
TatI	WGTACW	2 cut(s)	1257, 1566
Tru1I	TTAA	4 cut(s)	288, 651, 663, 1010
Tru9I	TTAA	4 cut(s)	288, 651, 663, 1010
TscAI	CASTG	6 cut(s)	444, 562, 693, 1054, 1275, 1282
TseFI	GTSAC	6 cut(s)	435, 458, 684, 1374, 1596, 1990
TseI	GCWGC	3 cut(s)	952, 1646, 1783
Tsp45I	GTSAC	6 cut(s)	435, 458, 684, 1374, 1596, 1990
TspDTI	ATGAA	5 cut(s)	111, 194, 531, 1078, 1997
TspGWI	ACGGA	4 cut(s)	110, 257, 857, 1649
TspRI	CASTG	6 cut(s)	444, 562, 693, 1054, 1275, 1282
Van91I	CCANNNNNTGG	1 cut(s)	728
VpaK11BI	GGWCC	3 cut(s)	1121, 1329, 1830
VspI	ATTAAT	1 cut(s)	1010
XagI	CCTNNNNNAGG	1 cut(s)	1931
XapI	RAATTY	3 cut(s)	925, 1522, 1699
XbaI	TCTAGA	1 cut(s)	487
XceI	RCATGY	3 cut(s)	620, 1212, 1573
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1010
XhoI	CTCGAG	1 cut(s)	2072
XmiI	GTMKAC	2 cut(s)	793, 1764
XspI	CTAG	4 cut(s)	488, 546, 756, 1440

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.