Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0004985

Physical Location & Seq

Genomic Coordinates Reverse (-)

36956460 .. 36961469

Gene Structure 5010 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1278700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2088 bp

ATGGGGGGTAGATGGAACCCTCCTGGGTTCCGGTTGCTATCTCTCCTGGTTTTGATTTTTGTTTCGAGGTTTCATGGTTGTTGGTGTCTCAATGATGAAGGATTGATTCTGTTGTCATTCTGCGAAAATATCAAGTTCGATCCTTTTGGTGCTTTGGAGAATTGGAGTCCTAATGATGCCGATCCTTGCCTGTGGAATGGTGTTCATTGTGTGGACGGTAATGTGCAGATGCTGAATCTGACGGATTTTTCTCTGGAAGGGACATTGGTACCTGAACTTGGGAAACTTAGTCACTTAAGATCTCTCGTGTTCTACAAGAACCGTTTATCTGGTGTAATTCCTAAAGAGATTGGGGGGCTCACAAGATTGGAACTGCTGGATTTGAGGAACAATAACTTGAGTGGGATGATTCCAGTTGAGTTAAGCAGTATGCTGTCACTGAAACGCTTGTTGCTTTGTGACAATAGATTCGAAGGCACCATTCCTCTTGAGCTCACTCGTCTCAGCTCGCTCTCTGAATTGCAATTTGATGATTACCTTACACCAAATGAAACCACTGGGTTTGGCTGTGTGAACAGAAAGTTTGGCCACTGCATCTGGCAGAGCAATTTAGAACGTGTGAATATGGTATTGTTTCTTAAAGGAGCTTTTAGGAACTATGTCAACTACTTGTCACTGCCACGGTTCCGTTACAAAAAGGAGTCTCTACGGATCGATGGAGATAATTGCTGTGATAATTTATCTAGTTTGTTTGAGTCGCACATGGCCTTTGCTCGCCGTAGACTACTTGCACAAGCCAGTAACCTACCAGCTTCACCTGTTACTGGCACAGAACCAACTGGACAGGTCATTGCTCTTCCAACTACCCGAAGTAGTGGCGCCTTCCCAGCTGTGCCAAAAGATAAAAGGAAAAATCCTCCTCCACCTGCACCTGCATCGCAGCCTTCACCCGAATCTCACGAGGCTTCAAAACATGATGGACAATCTTCCCAAAAATCAAATAGCAATATGTGGAAGTATCTCACTATTGCTTTCAGTGTGTTTATCTTGCTCCTTGTTCTTATTGCCATGGTCTTCATGTGTCGAACCCAAGTTGCTAAAACTGTAGGTCCCTGGAAGACTGGATTGAGTGGGCAGTTGCAGAAAGCATTTATAACAGGAGTTCCCAAGTTGAACCGACCAGAACTGGAAACCGCATGTGAAGATTTCAGTAACATTATTAGCACAATTGAAGGTTGCATTGTGTACAAGGGAACACTGTCGAGTGGAGTTGAAATTGCTGTGGCATCAACTACAATGTCGTCTTTGAAAGATTGGTCCAAGCATGCAGAGAATGCTTACCGGAAAAAGATTGACGTACTGTCACGCGTGAATCATAAGAACTTTGTCAATCTTATTGGCTACTGTGAGGAGGATGAACCTTTTACTAGGATGATGGTGTTTGAGTATGCTCCGAATGGAAATCTCTTTGAGCATCTGCATATTGAAGAAATGGAACACCTTGATTGGAATTCGAGGATTAGGATCGTTATGGGAACTGCTTATTGTCTTCAATACATGCACCATGAGCTAAACCCACCTGTGTCACATCCTAACCTGACCTCGGCTTCTATCTTTTTGACGGATGATTACGCTGCTAAGATTGCGGAGATCTGTTTCTGGGCAGAAGCAATACGAAAGCCTAAGAATTCCAGCGATGATGATAAGGAGCATTCGGTATTGCCACCCCTTGCTGATCCAGAAACAAACGTCTACAGTTTCGGAGTAATGCTACTTGAAATCATTTCCGGGAAGCTCCAAAACTCAGAAGAACATGGGTCCCTTCTATACTGGGCCTCTGCATATCTGAACGAGAATCGAAGCAACATGGTCGATCCAACCCTCAAGTCGTTCAAAAACGAAGAGCTGGATGTCCTGTGCGAGGTCATCAAAGATTGCATCCAAGAAGATCCAAGGCAGAGACCAACAATGAAGGATGTCACTAACAAACTGAGGGAAGTGATCCCCATCACTCCCAACCAAGCAGTCCCCAGACTCTCTCCCCTCTGGTGGGCGGAACTCGAGATCTTATCGGTCGAGGCGACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0021700 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042995 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071695 GO:0071704 GO:0071944 GO:0090406 GO:0090558 GO:0090627 GO:0099402 GO:0120025 GO:0140096 GO:1901564 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase_Tyr

Protein Analysis

696

Amino Acids

78.27

Weight (kDa)

5.92

Isoelectric Point (pI)

43.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 70	1.4e-10	Leucine rich repeat N-terminal domain
LRR_14	PF23598	77 - 176	4.2e-08	Leucine-rich repeat region
LRR_8	PF13855	97 - 157	7.5e-06	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	415 - 664	2.4e-20	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	415 - 662	5.8e-15	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18640 AT5G45840 AT5G45840
fragaria_vesca	FvH4_3g07660 FvH4_3g07660 FvH4_3g07660 FvH4_3g07660 FvH4_3g07660 FvH4_3g07660
malus_domestica	MD05G1299200.v1.1 MD10G1278700.v1.1
prunus_persica	Prupe.4G062600_v2.0.a1 Prupe.4G062600_v2.0.a1 Prupe.4G062600_v2.0.a1 Prupe.4G062600_v2.0.a1
pyrus_communis	pycom05g27710 pycom10g23270
rosa_chinensis	RchiOBHm_Chr5g0011021
rosa_laevigata	RLG00000031803
rosa_roxburghii	Rroxscaffold_1G00065380
rosa_rugosa	Rorug04G0454500 Rorug04G0454600 Rorug04G0454600 Rorug04G0454600
rosa_samantha	Rh5AG086800 Rh5BG081400 Rh5CG094200 Rh5DG081300
rosa_wichuraiana	Rw0G000120 Rw0G017830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1154
AarI	CACCTGC	2 cut(s)	934, 940
Acc36I	ACCTGC	2 cut(s)	934, 940
Acc65I	GGTACC	1 cut(s)	268
AccB1I	GGYRCC	3 cut(s)	268, 476, 878
AccI	GTMKAC	2 cut(s)	781, 1752
AccII	CGCG	1 cut(s)	1368
AciI	CCGC	3 cut(s)	1194, 1646, 2054
AclWI	GGATC	8 cut(s)	134, 176, 719, 1532, 1730, 1868, 1943, 1996
AcoI	YGGCCR	1 cut(s)	586
AcsI	RAATTY	2 cut(s)	1510, 1687
AcyI	GRCGYC	1 cut(s)	879
AfaI	GTAC	3 cut(s)	270, 1247, 1359
AfiI	CCNNNNNNNGG	6 cut(s)	278, 824, 1603, 1921, 2049, 2050
AflII	CTTAAG	1 cut(s)	295
AflIII	ACRYGT	2 cut(s)	616, 1366
AgsI	TTSAA	9 cut(s)	969, 1174, 1232, 1274, 1309, 1487, 1553, 1778, 1894
AhdI	GACNNNNNGTC	1 cut(s)	1360
AjnI	CCWGG	3 cut(s)	22, 45, 1112
AluBI	AGCT	8 cut(s)	493, 507, 647, 812, 890, 1570, 1795, 1906
AluI	AGCT	8 cut(s)	493, 507, 647, 812, 890, 1570, 1795, 1906
Alw21I	GWGCWC	1 cut(s)	495
Alw26I	GTCTC	4 cut(s)	92, 506, 708, 1954
AlwI	GGATC	8 cut(s)	134, 176, 719, 1532, 1730, 1868, 1943, 1996
AlwNI	CAGNNNCTG	1 cut(s)	232
Ama87I	CYCGRG	1 cut(s)	2060
AoxI	GGCC	3 cut(s)	586, 765, 1833
ApeKI	GCWGC	2 cut(s)	940, 1634
ApoI	RAATTY	2 cut(s)	1510, 1687
ArsI	GACNNNNNNTTYG	2 cut(s)	452, 484
Asp718I	GGTACC	1 cut(s)	268
AspLEI	GCGC	1 cut(s)	881
AspS9I	GGNCC	4 cut(s)	1109, 1317, 1818, 1833
AsuC2I	CCSGG	1 cut(s)	1789
AsuHPI	GGTGA	2 cut(s)	807, 939
AsuII	TTCGAA	1 cut(s)	471
AvaI	CYCGRG	1 cut(s)	2060
AvaII	GGWCC	3 cut(s)	1109, 1317, 1818
BaeI	ACNNNNGTAYC	2 cut(s)	252, 285
BalI	TGGCCA	1 cut(s)	588
BanI	GGYRCC	3 cut(s)	268, 476, 878
BanII	GRGCYC	2 cut(s)	360, 495
BauI	CACGAG	2 cut(s)	305, 959
BbsI	GAAGAC	3 cut(s)	1066, 1124, 1541
Bbv12I	GWGCWC	1 cut(s)	495
BbvI	GCAGC	2 cut(s)	952, 1621
BccI	CCATC	5 cut(s)	6, 710, 971, 1429, 2015
BceAI	ACGGC	1 cut(s)	762
BcgI	CGANNNNNNTGC	4 cut(s)	918, 952, 1852, 1886
BciT130I	CCWGG	3 cut(s)	24, 47, 1114
BcnI	CCSGG	1 cut(s)	1789
BcoDI	GTCTC	4 cut(s)	92, 506, 708, 1954
BfaI	CTAG	2 cut(s)	744, 1428
BfmI	CTRYAG	2 cut(s)	1104, 1753
BfoI	RGCGCY	1 cut(s)	882
BfrI	CTTAAG	1 cut(s)	295
BfuAI	ACCTGC	2 cut(s)	934, 940
BglII	AGATCT	3 cut(s)	299, 1650, 2064
BisI	GCNGC	2 cut(s)	941, 1635
BlsI	GCNGC	2 cut(s)	942, 1636
Bme1390I	CCNGG	4 cut(s)	24, 47, 1114, 1789
Bme18I	GGWCC	3 cut(s)	1109, 1317, 1818
BmeRI	GACNNNNNGTC	1 cut(s)	1360
BmeT110I	CYCGRG	1 cut(s)	2060
BmgT120I	GGNCC	4 cut(s)	1109, 1317, 1818, 1833
BmiI	GGNNCC	9 cut(s)	17, 29, 270, 478, 686, 880, 1111, 1819, 1820
BmrFI	CCNGG	4 cut(s)	24, 47, 1114, 1789
BmrI	ACTGGG	2 cut(s)	567, 1840
BmsI	GCATC	7 cut(s)	166, 219, 603, 944, 1295, 1483, 1947
BmuI	ACTGGG	2 cut(s)	567, 1840
BpiI	GAAGAC	3 cut(s)	1066, 1124, 1541
Bpu14I	TTCGAA	1 cut(s)	471
BpuEI	CTTGAG	3 cut(s)	418, 509, 1868
BpuMI	CCSGG	1 cut(s)	1789
Bsa29I	ATCGAT	1 cut(s)	714
BsaBI	GATNNNNATC	3 cut(s)	180, 1523, 2006
BsaHI	GRCGYC	1 cut(s)	879
BsaI	GGTCTC	1 cut(s)	1954
BsaJI	CCNNGG	6 cut(s)	23, 680, 1068, 1112, 1602, 1952
BsaWI	WCCGGW	2 cut(s)	30, 1341
Bsc4I	CCNNNNNNNGG	6 cut(s)	278, 824, 1603, 1921, 2049, 2050
Bse1I	ACTGG	8 cut(s)	413, 562, 798, 829, 844, 1126, 1191, 1835
Bse3DI	GCAATG	1 cut(s)	849
Bse8I	GATNNNNATC	3 cut(s)	180, 1523, 2006
BseBI	CCWGG	3 cut(s)	24, 47, 1114
BseCI	ATCGAT	1 cut(s)	714
BseDI	CCNNGG	6 cut(s)	23, 680, 1068, 1112, 1602, 1952
BseGI	GGATG	8 cut(s)	411, 1420, 1437, 1588, 1630, 1915, 1938, 1981
BseJI	GATNNNNATC	3 cut(s)	180, 1523, 2006
BseLI	CCNNNNNNNGG	6 cut(s)	278, 824, 1603, 1921, 2049, 2050
BseMI	GCAATG	1 cut(s)	849
BseMII	CTCAG	3 cut(s)	517, 1818, 1982
BseNI	ACTGG	8 cut(s)	413, 562, 798, 829, 844, 1126, 1191, 1835
BseRI	GAGGAG	2 cut(s)	909, 1424
BseXI	GCAGC	2 cut(s)	952, 1621
BseYI	CCCAGC	1 cut(s)	886
BsgI	GTGCAG	2 cut(s)	245, 912
Bsh1236I	CGCG	1 cut(s)	1368
Bsh1285I	CGRYCG	1 cut(s)	2076
BshFI	GGCC	3 cut(s)	588, 767, 1835
BshNI	GGYRCC	3 cut(s)	268, 476, 878
BshVI	ATCGAT	1 cut(s)	714
BsiEI	CGRYCG	1 cut(s)	2076
BsiHKAI	GWGCWC	1 cut(s)	495
BsiHKCI	CYCGRG	1 cut(s)	2060
BsiSI	CCGG	3 cut(s)	31, 1342, 1788
BslFI	GGGAC	4 cut(s)	274, 1095, 1804, 2012
BslI	CCNNNNNNNGG	6 cut(s)	278, 824, 1603, 1921, 2049, 2050
BsmAI	GTCTC	4 cut(s)	92, 506, 708, 1954
BsmBI	CGTCTC	1 cut(s)	506
BsmFI	GGGAC	4 cut(s)	274, 1095, 1804, 2012
BsmI	GAATGC	2 cut(s)	1339, 1711
BsnI	GGCC	3 cut(s)	588, 767, 1835
Bso31I	GGTCTC	1 cut(s)	1954
BsoBI	CYCGRG	1 cut(s)	2060
Bsp119I	TTCGAA	1 cut(s)	471
Bsp1286I	GDGCHC	2 cut(s)	360, 495
Bsp1407I	TGTACA	1 cut(s)	1245
Bsp19I	CCATGG	1 cut(s)	1068
BspACI	CCGC	3 cut(s)	1194, 1646, 2054
BspANI	GGCC	3 cut(s)	588, 767, 1835
BspCNI	CTCAG	3 cut(s)	516, 1817, 1983
BspDI	ATCGAT	1 cut(s)	714
BspFNI	CGCG	1 cut(s)	1368
BspLI	GGNNCC	9 cut(s)	17, 29, 270, 478, 686, 880, 1111, 1819, 1820
BspMI	ACCTGC	2 cut(s)	934, 940
BspPI	GGATC	8 cut(s)	134, 176, 719, 1532, 1730, 1868, 1943, 1996
BspQI	GCTCTTC	2 cut(s)	861, 1896
BspT104I	TTCGAA	1 cut(s)	471
BspT107I	GGYRCC	3 cut(s)	268, 476, 878
BspTI	CTTAAG	1 cut(s)	295
BspTNI	GGTCTC	1 cut(s)	1954
BsrDI	GCAATG	1 cut(s)	849
BsrGI	TGTACA	1 cut(s)	1245
BsrI	ACTGG	8 cut(s)	413, 562, 798, 829, 844, 1126, 1191, 1835
BssECI	CCNNGG	6 cut(s)	23, 680, 1068, 1112, 1602, 1952
BssNI	GRCGYC	1 cut(s)	879
BssSI	CACGAG	2 cut(s)	305, 959
BssT1I	CCWWGG	2 cut(s)	1068, 1952
Bst2BI	CACGAG	2 cut(s)	305, 959
Bst2UI	CCWGG	3 cut(s)	24, 47, 1114
Bst4CI	ACNGT	8 cut(s)	218, 323, 684, 1105, 1260, 1362, 1406, 1757
Bst6I	CTCTTC	2 cut(s)	861, 1896
BstACI	GRCGYC	1 cut(s)	879
BstAFI	CTTAAG	1 cut(s)	295
BstAPI	GCANNNNNTGC	1 cut(s)	1334
BstAUI	TGTACA	1 cut(s)	1245
BstBI	TTCGAA	1 cut(s)	471
BstC8I	GCNNGC	3 cut(s)	509, 775, 1326
BstDEI	CTNAG	6 cut(s)	287, 503, 1638, 1683, 1804, 1991
BstDSI	CCRYGG	2 cut(s)	680, 1068
BstENI	CCTNNNNNAGG	1 cut(s)	1919
BstF5I	GGATG	8 cut(s)	411, 1420, 1437, 1588, 1630, 1915, 1938, 1981
BstFNI	CGCG	1 cut(s)	1368
BstH2I	RGCGCY	1 cut(s)	882
BstHHI	GCGC	1 cut(s)	881
BstMAI	GTCTC	4 cut(s)	92, 506, 708, 1954
BstMCI	CGRYCG	1 cut(s)	2076
BstMWI	GCNNNNNNNGC	4 cut(s)	887, 1334, 1567, 1643
BstNI	CCWGG	3 cut(s)	24, 47, 1114
BstNSI	RCATGY	3 cut(s)	1200, 1328, 1561
BstSCI	CCNGG	4 cut(s)	22, 45, 1112, 1787
BstSFI	CTRYAG	2 cut(s)	1104, 1753
BstUI	CGCG	1 cut(s)	1368
BstV1I	GCAGC	2 cut(s)	952, 1621
BstV2I	GAAGAC	3 cut(s)	1066, 1124, 1541
BstX2I	RGATCY	4 cut(s)	299, 1650, 1948, 2064
BstYI	RGATCY	4 cut(s)	299, 1650, 1948, 2064
Bsu15I	ATCGAT	1 cut(s)	714
BsuRI	GGCC	3 cut(s)	588, 767, 1835
BsuTUI	ATCGAT	1 cut(s)	714
BtgI	CCRYGG	2 cut(s)	680, 1068
BtgZI	GCGATG	2 cut(s)	921, 1710
BtsCI	GGATG	8 cut(s)	411, 1420, 1437, 1588, 1630, 1915, 1938, 1981
BtsI	GCAGTG	2 cut(s)	589, 674
BtsIMutI	CAGTG	6 cut(s)	437, 555, 589, 674, 1042, 1256
BveI	ACCTGC	2 cut(s)	934, 940
Cac8I	GCNNGC	3 cut(s)	509, 775, 1326
CaiI	CAGNNNCTG	1 cut(s)	232
CfoI	GCGC	1 cut(s)	881
Cfr13I	GGNCC	4 cut(s)	1109, 1317, 1818, 1833
ClaI	ATCGAT	1 cut(s)	714
Csp6I	GTAC	3 cut(s)	269, 1246, 1358
CspCI	CAANNNNNGTGG	2 cut(s)	544, 579
CviQI	GTAC	3 cut(s)	269, 1246, 1358
DdeI	CTNAG	6 cut(s)	287, 503, 1638, 1683, 1804, 1991
DinI	GGCGCC	1 cut(s)	880
DriI	GACNNNNNGTC	1 cut(s)	1360
EaeI	YGGCCR	1 cut(s)	586
Eam1104I	CTCTTC	2 cut(s)	861, 1896
Eam1105I	GACNNNNNGTC	1 cut(s)	1360
EarI	CTCTTC	2 cut(s)	861, 1896
EciI	GGCGGA	1 cut(s)	2069
Ecl136II	GAGCTC	1 cut(s)	493
Eco130I	CCWWGG	2 cut(s)	1068, 1952
Eco24I	GRGCYC	2 cut(s)	360, 495
Eco31I	GGTCTC	1 cut(s)	1954
Eco47I	GGWCC	3 cut(s)	1109, 1317, 1818
Eco53kI	GAGCTC	1 cut(s)	493
Eco88I	CYCGRG	1 cut(s)	2060
EcoICRI	GAGCTC	1 cut(s)	493
EcoNI	CCTNNNNNAGG	1 cut(s)	1919
EcoO109I	RGGNCCY	2 cut(s)	1109, 1818
EcoRI	GAATTC	2 cut(s)	1510, 1687
EcoRII	CCWGG	3 cut(s)	22, 45, 1112
EcoT14I	CCWWGG	2 cut(s)	1068, 1952
EcoT38I	GRGCYC	2 cut(s)	360, 495
EgeI	GGCGCC	1 cut(s)	880
EheI	GGCGCC	1 cut(s)	880
ErhI	CCWWGG	2 cut(s)	1068, 1952
Esp3I	CGTCTC	1 cut(s)	506
FaqI	GGGAC	4 cut(s)	274, 1095, 1804, 2012
FblI	GTMKAC	2 cut(s)	781, 1752
Fnu4HI	GCNGC	2 cut(s)	941, 1635
FokI	GGATG	8 cut(s)	418, 1427, 1444, 1575, 1637, 1922, 1925, 1988
FriOI	GRGCYC	2 cut(s)	360, 495
Fsp4HI	GCNGC	2 cut(s)	941, 1635
FspBI	CTAG	2 cut(s)	744, 1428
GlaI	GCGC	1 cut(s)	880
GluI	GCNGC	2 cut(s)	941, 1635
GsaI	CCCAGC	1 cut(s)	890
HaeII	RGCGCY	1 cut(s)	882
HaeIII	GGCC	3 cut(s)	588, 767, 1835
HapII	CCGG	3 cut(s)	31, 1342, 1788
HhaI	GCGC	1 cut(s)	881
Hin1I	GRCGYC	1 cut(s)	879
Hin6I	GCGC	1 cut(s)	879
HinP1I	GCGC	1 cut(s)	879
HincII	GTYRAC	1 cut(s)	664
HindII	GTYRAC	1 cut(s)	664
HpaII	CCGG	3 cut(s)	31, 1342, 1788
HphI	GGTGA	2 cut(s)	807, 939
Hpy166II	GTNNAC	6 cut(s)	214, 574, 664, 782, 1246, 1753
Hpy188I	TCNGA	6 cut(s)	240, 517, 1455, 1763, 1807, 1848
Hpy188III	TCNNGA	5 cut(s)	254, 488, 959, 1739, 2062
Hpy8I	GTNNAC	6 cut(s)	214, 574, 664, 782, 1246, 1753
HpyAV	CCTTC	8 cut(s)	92, 251, 467, 892, 954, 1226, 1832, 1966
HpyCH4III	ACNGT	8 cut(s)	218, 323, 684, 1105, 1260, 1362, 1406, 1757
HpyCH4IV	ACGT	3 cut(s)	616, 1356, 1749
HpyF10VI	GCNNNNNNNGC	4 cut(s)	887, 1334, 1567, 1643
HpyF3I	CTNAG	6 cut(s)	287, 503, 1638, 1683, 1804, 1991
HpySE526I	ACGT	3 cut(s)	616, 1356, 1749
Hsp92I	GRCGYC	1 cut(s)	879
HspAI	GCGC	1 cut(s)	879
KasI	GGCGCC	1 cut(s)	878
KflI	GGGWCCC	1 cut(s)	1818
KpnI	GGTACC	1 cut(s)	272
LguI	GCTCTTC	2 cut(s)	861, 1896
LmnI	GCTCC	5 cut(s)	644, 1056, 1456, 1708, 1800
Lsp1109I	GCAGC	2 cut(s)	952, 1621
LweI	GCATC	7 cut(s)	166, 219, 603, 944, 1295, 1483, 1947
MaeI	CTAG	2 cut(s)	744, 1428
MaeII	ACGT	3 cut(s)	616, 1356, 1749
MfeI	CAATTG	1 cut(s)	1227
MflI	RGATCY	4 cut(s)	299, 1650, 1948, 2064
MhlI	GDGCHC	2 cut(s)	360, 495
MlsI	TGGCCA	1 cut(s)	588
MluI	ACGCGT	1 cut(s)	1366
MluNI	TGGCCA	1 cut(s)	588
Mly113I	GGCGCC	1 cut(s)	879
MlyI	GAGTC	4 cut(s)	175, 710, 764, 2028
MmeI	TCCRAC	2 cut(s)	884, 1901
Mox20I	TGGCCA	1 cut(s)	588
MscI	TGGCCA	1 cut(s)	588
MseI	TTAA	3 cut(s)	296, 422, 639
Msp20I	TGGCCA	1 cut(s)	588
MspA1I	CMGCKG	1 cut(s)	890
MspCI	CTTAAG	1 cut(s)	295
MspI	CCGG	3 cut(s)	31, 1342, 1788
MspR9I	CCNGG	4 cut(s)	24, 47, 1114, 1789
MunI	CAATTG	1 cut(s)	1227
Mva1269I	GAATGC	2 cut(s)	1339, 1711
MvaI	CCWGG	3 cut(s)	24, 47, 1114
MvnI	CGCG	1 cut(s)	1368
MwoI	GCNNNNNNNGC	4 cut(s)	887, 1334, 1567, 1643
NarI	GGCGCC	1 cut(s)	879
NciI	CCSGG	1 cut(s)	1789
NcoI	CCATGG	1 cut(s)	1068
NlaIV	GGNNCC	9 cut(s)	17, 29, 270, 478, 686, 880, 1111, 1819, 1820
NmeAIII	GCCGAG	1 cut(s)	1583
NmuCI	GTSAC	7 cut(s)	290, 435, 458, 672, 1362, 1584, 1978
NspI	RCATGY	3 cut(s)	1200, 1328, 1561
NspV	TTCGAA	1 cut(s)	471
PaeI	GCATGC	1 cut(s)	1328
PaeR7I	CTCGAG	1 cut(s)	2060
PaqCI	CACCTGC	2 cut(s)	934, 940
PciSI	GCTCTTC	2 cut(s)	861, 1896
PcsI	WCGNNNNNNNCGW	1 cut(s)	2078
PctI	GAATGC	2 cut(s)	1339, 1711
PfeI	GAWTC	7 cut(s)	106, 235, 409, 468, 953, 1372, 1855
PfoI	TCCNGGA	1 cut(s)	1787
PkrI	GCNGC	2 cut(s)	942, 1636
PleI	GAGTC	4 cut(s)	174, 709, 763, 2028
PluTI	GGCGCC	1 cut(s)	882
PpsI	GAGTC	4 cut(s)	174, 709, 763, 2028
PpuMI	RGGWCCY	2 cut(s)	1109, 1818
PsiI	TTATAA	1 cut(s)	1154
Psp124BI	GAGCTC	1 cut(s)	495
Psp5II	RGGWCCY	2 cut(s)	1109, 1818
Psp6I	CCWGG	3 cut(s)	22, 45, 1112
PspFI	CCCAGC	1 cut(s)	886
PspGI	CCWGG	3 cut(s)	22, 45, 1112
PspN4I	GGNNCC	9 cut(s)	17, 29, 270, 478, 686, 880, 1111, 1819, 1820
PspPI	GGNCC	4 cut(s)	1109, 1317, 1818, 1833
PspPPI	RGGWCCY	2 cut(s)	1109, 1818
PstNI	CAGNNNCTG	1 cut(s)	232
PsuI	RGATCY	4 cut(s)	299, 1650, 1948, 2064
PvuII	CAGCTG	1 cut(s)	890
RsaI	GTAC	3 cut(s)	270, 1247, 1359
RsaNI	GTAC	3 cut(s)	269, 1246, 1358
SacI	GAGCTC	1 cut(s)	495
SapI	GCTCTTC	2 cut(s)	861, 1896
SaqAI	TTAA	3 cut(s)	296, 422, 639
SatI	GCNGC	2 cut(s)	941, 1635
Sau96I	GGNCC	4 cut(s)	1109, 1317, 1818, 1833
SchI	GAGTC	4 cut(s)	175, 710, 764, 2028
ScrFI	CCNGG	4 cut(s)	24, 47, 1114, 1789
SduI	GDGCHC	2 cut(s)	360, 495
SfaNI	GCATC	7 cut(s)	166, 219, 603, 944, 1295, 1483, 1947
SfcI	CTRYAG	2 cut(s)	1104, 1753
SfoI	GGCGCC	1 cut(s)	880
Sfr274I	CTCGAG	1 cut(s)	2060
SfuI	TTCGAA	1 cut(s)	471
SinI	GGWCC	3 cut(s)	1109, 1317, 1818
SlaI	CTCGAG	1 cut(s)	2060
SmlI	CTYRAG	5 cut(s)	295, 397, 488, 1883, 2060
SmoI	CTYRAG	5 cut(s)	295, 397, 488, 1883, 2060
SphI	GCATGC	1 cut(s)	1328
SsiI	CCGC	3 cut(s)	1194, 1646, 2054
SspDI	GGCGCC	1 cut(s)	878
SspMI	CTAG	2 cut(s)	744, 1428
SstI	GAGCTC	1 cut(s)	495
StyD4I	CCNGG	4 cut(s)	22, 45, 1112, 1787
StyI	CCWWGG	2 cut(s)	1068, 1952
TaaI	ACNGT	8 cut(s)	218, 323, 684, 1105, 1260, 1362, 1406, 1757
TaiI	ACGT	3 cut(s)	619, 1359, 1752
TaqII	GACCGA	1 cut(s)	2062
TatI	WGTACW	1 cut(s)	1245
TfiI	GAWTC	7 cut(s)	106, 235, 409, 468, 953, 1372, 1855
Tru1I	TTAA	3 cut(s)	296, 422, 639
Tru9I	TTAA	3 cut(s)	296, 422, 639
TscAI	CASTG	6 cut(s)	444, 562, 596, 681, 1042, 1263
TseFI	GTSAC	7 cut(s)	290, 435, 458, 672, 1362, 1584, 1978
TseI	GCWGC	2 cut(s)	940, 1634
Tsp45I	GTSAC	7 cut(s)	290, 435, 458, 672, 1362, 1584, 1978
TspDTI	ATGAA	7 cut(s)	62, 111, 194, 564, 1066, 1431, 1985
TspGWI	ACGGA	4 cut(s)	257, 677, 724, 1637
TspRI	CASTG	6 cut(s)	444, 562, 596, 681, 1042, 1263
Vha464I	CTTAAG	1 cut(s)	295
VpaK11BI	GGWCC	3 cut(s)	1109, 1317, 1818
XagI	CCTNNNNNAGG	1 cut(s)	1919
XapI	RAATTY	2 cut(s)	1510, 1687
XceI	RCATGY	3 cut(s)	1200, 1328, 1561
XhoI	CTCGAG	1 cut(s)	2060
XmiI	GTMKAC	2 cut(s)	781, 1752
XspI	CTAG	2 cut(s)	744, 1428

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.