MD05G1325100.v1.1

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011534
Physical Location & Seq
Forward (+)
45145339 .. 45149859
4521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1325100.v1.1.491

Sequence Viewer

Length: 1914 bp
ATGCAGACGAGGTACATGGAGAGATCGAATAGCATGGCGAGGGAGAAACGAAGTTTGGATTCGTCTTCAGCTGAAGAAGGCCAGCCGGATAGGAAACGGCCTGCTCTCGCCAGTGTAATTGTTGAAGCTCTCAAGGTGGATAGTCTGCAGAAGCTTTGCTCGTCACTGGAGCCGATTCTACGCAGAGTTGTTAGTGAAGAGGTGGAGCGTGCTTTAGCAAAATTAGGCCCTGCCAAACTTACTGGAAGGTCTTCTCCTAAACAAATTGGAGGGCCCGATGGACGTGACTTGCAGCTGCACTTTAGGTCCAGGTTGTCCCTTCCTCTTTTTACTGGTGGAAAAGTAGAAGGGGAGTGGGGTTCTGCAATCCCTATTGTCTTGATTGATGCAAATACAAAGCATGTTGTGACATCAGGCCCGGAGTCAGCAGTGAAACTTGACGTTGTTGTGCTTGAAGGTGATTTTAACAACGAGGATGATGAGAACTGGACTGAAGAAGAATTTGAGAGCCATGTAGTAAAAGAGCGTGAAGGGAAGAGGCCACTTCTTACTGGCGATCTGCAAGTGACACTGAAGGAAGGTGTAGGAACACTAGGGGAATTGACATTTACCGATAATTCTAGCTGGATTAGGAGCAGGAAGTTTAGGCTAGGACTGAAAGTTGCCTCAGGCTATTGCGACAACATTCGTATACGCGAAGCAAAAACAGATGCCTTTACTGTTAAGGATCACCGTGGGGAATTATACAAGAAACACTACCCTCCTGCGTTAAATGATGAGGTCTGGAGATTGGAGAAAATTGGAAAAGATGGCTCATTCCACAAGAGACTGAATAAAGCTGGAATTGTTACAGTTGAAGACTTCCTGCGACTTGTGGTTAGAGACTCGCAGAGATTGCGGAATATTCTTGGAAGTGGCATGTCGAATAAGATGTGGGATGTACTCATACAGCATGCAAAAACCTGTCTTCTAGGCGGGAAACTCTATGTCTACTATCCTGAGGATGCAAGGAACGTTGGTGTTGTTTTTAACAATATCTACGAGTTGAGTGGCCTAATTACCAATGAACAATTTTACTCAGCTGATTCTCTCTCGGACGCTCAGAAGGTCTACGTGGACGGTTTGGTAACAAAGGCATATGAGAACTGGATGCATGTTATGGAGTACGATGGAAAGTCTCTTCTTAAGCAGGAGAAGAGTCCCGATGTTTCTCTATCTGAGGTCCCAACGGCCTCGCAGGATTATCCAAACTCATTTGATCAGCAGTTCACTCTACCTAGCCTGCCAGCTTCAGTTTCTTCAGAACCGCCTACTATGGATTCTGGCCTAAATGTGGGAGGTTATACTGACGGCATGGCTAACAGATTCTCGGTAGAGTCACAGAATCTAAATCTCAGTGCTCCCAGTCAGCTCGATGGCTTGCCATTCCCTCTACAAAATCAGCAGCCTATCACTTCACACCAGGCTCAGTTTCAAAGAAATGAAAACATGCTTGCCCTTGGTCCACCACAGTCGTCCACATCTGGGTTCCAGAATATTGGTACATCCAATCCGTCTTCTTATAGGGGAGCCGAGGATCTCTTCCCAGAGGAAGAAATTCGTATGAGGAGCCACGAGATTCTCGAAAATGAAGATATGCAGCGTCTACTTCATATCTTTAACATGGGCGGTCAGGGTCAAGGCCACGGTTATGGTCATGCCTCCATGAACATCACTGAAGACAATTATCCATATTCGTCACCATACGTGCCCACTCCACAAGTGAACTACAGTGTTGATGATGATCATAGCCGTTCGTCTGGGAAAGCTGTTGTTGGCTGGCTCAAGCTCAAGGCAGCCCTTAGATGGGGCATATTCATCAGAAAGAAGGCTGCTGAGAGACGGGCACAGCTTGTTGAGTTGGATGATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

638

Amino Acids

71.15

Weight (kDa)

5.74

Isoelectric Point (pI)

54.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 97 - 244 6.4e-63 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 256 - 322 3.4e-33 Calmodulin binding protein central domain
Calmod_bind_C PF20452 327 - 389 2.5e-22 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 691, 990, 1110, 1645
AccII CGCG 1 cut(s) 696
AciI CCGC 4 cut(s) 898, 975, 1307, 1668
AclI AACGTT 1 cut(s) 1014
AclWI GGATC 2 cut(s) 735, 1584
AcsI RAATTY 2 cut(s) 500, 1596
AcuI CTGAAG 7 cut(s) 51, 93, 513, 593, 1275, 1284, 1737
AfaI GTAC 4 cut(s) 14, 942, 1166, 1543
AfiI CCNNNNNNNGG 4 cut(s) 1333, 1524, 1845, 1846
AflII CTTAAG 1 cut(s) 1184
AgsI TTSAA 4 cut(s) 125, 455, 857, 1475
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 2 cut(s) 308, 1461
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
Alw21I GWGCWC 1 cut(s) 1402
Alw26I GTCTC 4 cut(s) 820, 876, 1182, 1873
AlwI GGATC 2 cut(s) 735, 1584
ApaI GGGCCC 1 cut(s) 276
ApeKI GCWGC 6 cut(s) 292, 295, 1444, 1639, 1835, 1871
ApoI RAATTY 2 cut(s) 500, 1596
Asp700I GAANNNNTTC 2 cut(s) 250, 1596
AspS9I GGNCC 7 cut(s) 227, 272, 273, 306, 416, 1222, 1502
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 3 cut(s) 470, 722, 1731
AvaII GGWCC 3 cut(s) 306, 1222, 1502
AxyI CCTNAGG 2 cut(s) 667, 999
BaeGI GKGCMC 3 cut(s) 276, 1752, 1888
BanII GRGCYC 1 cut(s) 276
BauI CACGAG 1 cut(s) 1613
BbsI GAAGAC 6 cut(s) 57, 243, 864, 959, 1548, 1725
Bbv12I GWGCWC 1 cut(s) 1402
BbvI GCAGC 6 cut(s) 282, 304, 1456, 1651, 1847, 1858
BccI CCATC 5 cut(s) 272, 803, 1163, 1409, 1839
BceAI ACGGC 4 cut(s) 113, 1245, 1366, 1776
BciT130I CCWGG 2 cut(s) 310, 1463
BclI TGATCA 2 cut(s) 1258, 1783
BcnI CCSGG 1 cut(s) 419
BcoDI GTCTC 4 cut(s) 820, 876, 1182, 1873
BfaI CTAG 5 cut(s) 593, 621, 650, 971, 1278
BfmI CTRYAG 2 cut(s) 146, 1768
BfrI CTTAAG 1 cut(s) 1184
BisI GCNGC 6 cut(s) 293, 296, 1445, 1640, 1836, 1872
BlsI GCNGC 6 cut(s) 294, 297, 1446, 1641, 1837, 1873
Bme1390I CCNGG 3 cut(s) 310, 419, 1463
Bme18I GGWCC 3 cut(s) 306, 1222, 1502
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 7 cut(s) 227, 272, 273, 306, 416, 1222, 1502
BmiI GGNNCC 6 cut(s) 171, 274, 1224, 1529, 1570, 1610
BmrFI CCNGG 3 cut(s) 310, 419, 1463
BmrI ACTGGG 1 cut(s) 1398
BmsI GCATC 4 cut(s) 376, 700, 994, 1140
BmuI ACTGGG 1 cut(s) 1398
BpiI GAAGAC 6 cut(s) 57, 243, 864, 959, 1548, 1725
BpmI CTGGAG 2 cut(s) 188, 805
BpuEI CTTGAG 3 cut(s) 116, 1808, 1814
BpuMI CCSGG 1 cut(s) 419
BsaAI YACGTR 2 cut(s) 1114, 1747
BsaBI GATNNNNATC 1 cut(s) 1782
BsaJI CCNNGG 4 cut(s) 733, 1498, 1572, 1684
Bsc4I CCNNNNNNNGG 4 cut(s) 1333, 1524, 1845, 1846
Bse1I ACTGG 8 cut(s) 111, 171, 247, 337, 491, 556, 1151, 1404
Bse21I CCTNAGG 2 cut(s) 667, 999
Bse8I GATNNNNATC 1 cut(s) 1782
BseBI CCWGG 2 cut(s) 310, 1463
BseDI CCNNGG 4 cut(s) 733, 1498, 1572, 1684
BseGI GGATG 6 cut(s) 481, 943, 1009, 1155, 1544, 1909
BseJI GATNNNNATC 1 cut(s) 1782
BseLI CCNNNNNNNGG 4 cut(s) 1333, 1524, 1845, 1846
BseMII CTCAG 8 cut(s) 681, 990, 1092, 1115, 1209, 1408, 1481, 1866
BseNI ACTGG 8 cut(s) 111, 171, 247, 337, 491, 556, 1151, 1404
BseRI GAGGAG 1 cut(s) 1621
BseSI GKGCMC 3 cut(s) 276, 1752, 1888
BseXI GCAGC 6 cut(s) 282, 304, 1456, 1651, 1847, 1858
BsgI GTGCAG 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 1402
BsiSI CCGG 2 cut(s) 86, 419
BslFI GGGAC 3 cut(s) 301, 1185, 1208
BslI CCNNNNNNNGG 4 cut(s) 1333, 1524, 1845, 1846
BsmAI GTCTC 4 cut(s) 820, 876, 1182, 1873
BsmBI CGTCTC 1 cut(s) 1873
BsmFI GGGAC 3 cut(s) 301, 1185, 1208
Bsp120I GGGCCC 1 cut(s) 272
Bsp1286I GDGCHC 4 cut(s) 276, 1402, 1752, 1888
Bsp143I GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
BspACI CCGC 4 cut(s) 898, 975, 1307, 1668
BspCNI CTCAG 8 cut(s) 680, 991, 1091, 1114, 1210, 1407, 1480, 1867
BspFNI CGCG 1 cut(s) 696
BspLI GGNNCC 6 cut(s) 171, 274, 1224, 1529, 1570, 1610
BspMAI CTGCAG 1 cut(s) 150
BspPI GGATC 2 cut(s) 735, 1584
BspTI CTTAAG 1 cut(s) 1184
BsrI ACTGG 8 cut(s) 111, 171, 247, 337, 491, 556, 1151, 1404
BssECI CCNNGG 4 cut(s) 733, 1498, 1572, 1684
BssMI GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
BssNAI GTATAC 1 cut(s) 692
BssSI CACGAG 1 cut(s) 1613
BssT1I CCWWGG 1 cut(s) 1498
Bst1107I GTATAC 1 cut(s) 692
Bst2BI CACGAG 1 cut(s) 1613
Bst2UI CCWGG 2 cut(s) 310, 1463
Bst4CI ACNGT 7 cut(s) 721, 734, 853, 1121, 1512, 1688, 1772
Bst6I CTCTTC 5 cut(s) 192, 530, 1185, 1190, 1586
BstAFI CTTAAG 1 cut(s) 1184
BstAPI GCANNNNNTGC 1 cut(s) 895
BstBAI YACGTR 2 cut(s) 1114, 1747
BstC8I GCNNGC 9 cut(s) 83, 102, 210, 954, 1283, 1287, 1421, 1494, 1820
BstDEI CTNAG 9 cut(s) 667, 999, 1078, 1101, 1218, 1394, 1467, 1841, 1875
BstDSI CCRYGG 2 cut(s) 733, 1684
BstF5I GGATG 6 cut(s) 481, 943, 1009, 1155, 1544, 1909
BstFNI CGCG 1 cut(s) 696
BstKTI GATC 6 cut(s) 26, 559, 730, 1261, 1579, 1786
BstMAI GTCTC 4 cut(s) 820, 876, 1182, 1873
BstMBI GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
BstMWI GCNNNNNNNGC 1 cut(s) 895
BstNI CCWGG 2 cut(s) 310, 1463
BstNSI RCATGY 5 cut(s) 404, 922, 956, 1157, 1492
BstSCI CCNGG 3 cut(s) 308, 417, 1461
BstSFI CTRYAG 2 cut(s) 146, 1768
BstSLI GKGCMC 3 cut(s) 276, 1752, 1888
BstUI CGCG 1 cut(s) 696
BstV1I GCAGC 6 cut(s) 282, 304, 1456, 1651, 1847, 1858
BstV2I GAAGAC 6 cut(s) 57, 243, 864, 959, 1548, 1725
BstX2I RGATCY 1 cut(s) 1576
BstXI CCANNNNNNTGG 2 cut(s) 1538, 1691
BstYI RGATCY 1 cut(s) 1576
BstZ17I GTATAC 1 cut(s) 692
Bsu36I CCTNAGG 2 cut(s) 667, 999
BtgI CCRYGG 2 cut(s) 733, 1684
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 6 cut(s) 481, 943, 1009, 1155, 1544, 1909
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 7 cut(s) 118, 164, 435, 569, 1402, 1713, 1777
Cac8I GCNNGC 9 cut(s) 83, 102, 210, 954, 1283, 1287, 1421, 1494, 1820
Cfr13I GGNCC 7 cut(s) 227, 272, 273, 306, 416, 1222, 1502
CseI GACGC 2 cut(s) 1106, 1631
Csp6I GTAC 4 cut(s) 13, 941, 1165, 1542
CviQI GTAC 4 cut(s) 13, 941, 1165, 1542
DdeI CTNAG 9 cut(s) 667, 999, 1078, 1101, 1218, 1394, 1467, 1841, 1875
DpnI GATC 6 cut(s) 25, 558, 729, 1260, 1578, 1785
DpnII GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
Eam1104I CTCTTC 5 cut(s) 192, 530, 1185, 1190, 1586
EarI CTCTTC 5 cut(s) 192, 530, 1185, 1190, 1586
Eco130I CCWWGG 1 cut(s) 1498
Eco24I GRGCYC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 306, 1222, 1502
Eco57I CTGAAG 7 cut(s) 51, 93, 513, 593, 1275, 1284, 1737
Eco81I CCTNAGG 2 cut(s) 667, 999
EcoO109I RGGNCCY 3 cut(s) 227, 272, 1222
EcoRII CCWGG 2 cut(s) 308, 1461
EcoT14I CCWWGG 1 cut(s) 1498
EcoT22I ATGCAT 1 cut(s) 1155
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 1498
Esp3I CGTCTC 1 cut(s) 1873
FaqI GGGAC 3 cut(s) 301, 1185, 1208
FauI CCCGC 1 cut(s) 968
FauNDI CATATG 1 cut(s) 1138
FbaI TGATCA 2 cut(s) 1258, 1783
FblI GTMKAC 4 cut(s) 691, 990, 1110, 1645
Fnu4HI GCNGC 6 cut(s) 293, 296, 1445, 1640, 1836, 1872
FokI GGATG 5 cut(s) 488, 950, 1016, 1162, 1531
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 6 cut(s) 293, 296, 1445, 1640, 1836, 1872
FspBI CTAG 5 cut(s) 593, 621, 650, 971, 1278
GluI GCNGC 6 cut(s) 293, 296, 1445, 1640, 1836, 1872
GsuI CTGGAG 2 cut(s) 188, 805
HapII CCGG 2 cut(s) 86, 419
HgaI GACGC 2 cut(s) 1106, 1631
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 2 cut(s) 86, 419
HphI GGTGA 3 cut(s) 470, 722, 1731
Hpy166II GTNNAC 9 cut(s) 692, 991, 1111, 1117, 1269, 1505, 1518, 1646, 1765
Hpy188I TCNGA 5 cut(s) 1096, 1104, 1219, 1303, 1862
Hpy188III TCNNGA 6 cut(s) 379, 784, 998, 1202, 1531, 1622
Hpy8I GTNNAC 9 cut(s) 692, 991, 1111, 1117, 1269, 1505, 1518, 1646, 1765
HpyCH4III ACNGT 7 cut(s) 721, 734, 853, 1121, 1512, 1688, 1772
HpyCH4IV ACGT 5 cut(s) 283, 441, 1014, 1113, 1746
HpyF10VI GCNNNNNNNGC 1 cut(s) 895
HpyF3I CTNAG 9 cut(s) 667, 999, 1078, 1101, 1218, 1394, 1467, 1841, 1875
HpySE526I ACGT 5 cut(s) 283, 441, 1014, 1113, 1746
Ksp22I TGATCA 2 cut(s) 1258, 1783
Kzo9I GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
LmnI GCTCC 6 cut(s) 169, 205, 633, 1405, 1568, 1608
Lsp1109I GCAGC 6 cut(s) 282, 304, 1456, 1651, 1847, 1858
LweI GCATC 4 cut(s) 376, 700, 994, 1140
MaeI CTAG 5 cut(s) 593, 621, 650, 971, 1278
MaeII ACGT 5 cut(s) 283, 441, 1014, 1113, 1746
MaeIII GTNAC 8 cut(s) 162, 284, 406, 565, 847, 1126, 1377, 1737
MalI GATC 6 cut(s) 25, 558, 729, 1260, 1578, 1785
MboI GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
MflI RGATCY 1 cut(s) 1576
MhlI GDGCHC 4 cut(s) 276, 1402, 1752, 1888
MlyI GAGTC 4 cut(s) 431, 878, 1207, 1385
MmeI TCCRAC 1 cut(s) 1881
Mph1103I ATGCAT 1 cut(s) 1155
MroXI GAANNNNTTC 2 cut(s) 250, 1596
MseI TTAA 6 cut(s) 465, 723, 770, 1029, 1185, 1659
MslI CAYNNNNRTG 1 cut(s) 1689
MspA1I CMGCKG 3 cut(s) 71, 295, 1082
MspCI CTTAAG 1 cut(s) 1184
MspI CCGG 2 cut(s) 86, 419
MspR9I CCNGG 3 cut(s) 310, 419, 1463
MvaI CCWGG 2 cut(s) 310, 1463
MvnI CGCG 1 cut(s) 696
MwoI GCNNNNNNNGC 1 cut(s) 895
NciI CCSGG 1 cut(s) 419
NdeI CATATG 1 cut(s) 1138
NdeII GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
NlaIV GGNNCC 6 cut(s) 171, 274, 1224, 1529, 1570, 1610
NmeAIII GCCGAG 1 cut(s) 1597
NmuCI GTSAC 6 cut(s) 162, 284, 406, 565, 1377, 1737
NsiI ATGCAT 1 cut(s) 1155
NspI RCATGY 5 cut(s) 404, 922, 956, 1157, 1492
PaeI GCATGC 1 cut(s) 956
PcsI WCGNNNNNNNCGW 1 cut(s) 1620
PdmI GAANNNNTTC 2 cut(s) 250, 1596
PfeI GAWTC 8 cut(s) 59, 175, 1085, 1319, 1365, 1384, 1618, 1907
PkrI GCNGC 6 cut(s) 294, 297, 1446, 1641, 1837, 1873
PleI GAGTC 4 cut(s) 430, 878, 1206, 1384
PpsI GAGTC 4 cut(s) 430, 878, 1206, 1384
Ppu21I YACGTR 2 cut(s) 1114, 1747
PpuMI RGGWCCY 1 cut(s) 1222
Psp1406I AACGTT 1 cut(s) 1014
Psp5II RGGWCCY 1 cut(s) 1222
Psp6I CCWGG 2 cut(s) 308, 1461
PspGI CCWGG 2 cut(s) 308, 1461
PspN4I GGNNCC 6 cut(s) 171, 274, 1224, 1529, 1570, 1610
PspOMI GGGCCC 1 cut(s) 272
PspPI GGNCC 7 cut(s) 227, 272, 273, 306, 416, 1222, 1502
PspPPI RGGWCCY 1 cut(s) 1222
PstI CTGCAG 1 cut(s) 150
PsuI RGATCY 1 cut(s) 1576
PvuII CAGCTG 3 cut(s) 71, 295, 1082
RsaI GTAC 4 cut(s) 14, 942, 1166, 1543
RsaNI GTAC 4 cut(s) 13, 941, 1165, 1542
RseI CAYNNNNRTG 1 cut(s) 1689
SaqAI TTAA 6 cut(s) 465, 723, 770, 1029, 1185, 1659
SatI GCNGC 6 cut(s) 293, 296, 1445, 1640, 1836, 1872
Sau3AI GATC 6 cut(s) 23, 556, 727, 1258, 1576, 1783
Sau96I GGNCC 7 cut(s) 227, 272, 273, 306, 416, 1222, 1502
SchI GAGTC 4 cut(s) 431, 878, 1207, 1385
ScrFI CCNGG 3 cut(s) 310, 419, 1463
SduI GDGCHC 4 cut(s) 276, 1402, 1752, 1888
SfaNI GCATC 4 cut(s) 376, 700, 994, 1140
SfcI CTRYAG 2 cut(s) 146, 1768
SinI GGWCC 3 cut(s) 306, 1222, 1502
SmiMI CAYNNNNRTG 1 cut(s) 1689
SmlI CTYRAG 4 cut(s) 131, 1184, 1823, 1829
SmoI CTYRAG 4 cut(s) 131, 1184, 1823, 1829
SphI GCATGC 1 cut(s) 956
SsiI CCGC 4 cut(s) 898, 975, 1307, 1668
SspI AATATT 2 cut(s) 904, 1537
SspMI CTAG 5 cut(s) 593, 621, 650, 971, 1278
StyD4I CCNGG 3 cut(s) 308, 417, 1461
StyI CCWWGG 1 cut(s) 1498
TaaI ACNGT 7 cut(s) 721, 734, 853, 1121, 1512, 1688, 1772
TaiI ACGT 5 cut(s) 286, 444, 1017, 1116, 1749
TaqI TCGA 4 cut(s) 26, 923, 1413, 1623
TatI WGTACW 1 cut(s) 940
TfiI GAWTC 8 cut(s) 59, 175, 1085, 1319, 1365, 1384, 1618, 1907
Tru1I TTAA 6 cut(s) 465, 723, 770, 1029, 1185, 1659
Tru9I TTAA 6 cut(s) 465, 723, 770, 1029, 1185, 1659
TscAI CASTG 7 cut(s) 118, 171, 435, 576, 1402, 1720, 1777
TseFI GTSAC 6 cut(s) 162, 284, 406, 565, 1377, 1737
TseI GCWGC 6 cut(s) 292, 295, 1444, 1639, 1835, 1871
Tsp45I GTSAC 6 cut(s) 162, 284, 406, 565, 1377, 1737
TspDTI ATGAA 7 cut(s) 1080, 1497, 1640, 1644, 1721, 1846, 1899
TspGWI ACGGA 1 cut(s) 1542
TspRI CASTG 7 cut(s) 118, 171, 435, 576, 1402, 1720, 1777
Vha464I CTTAAG 1 cut(s) 1184
VpaK11BI GGWCC 3 cut(s) 306, 1222, 1502
XapI RAATTY 2 cut(s) 500, 1596
XceI RCATGY 5 cut(s) 404, 922, 956, 1157, 1492
XmiI GTMKAC 4 cut(s) 691, 990, 1110, 1645
XmnI GAANNNNTTC 2 cut(s) 250, 1596
XspI CTAG 5 cut(s) 593, 621, 650, 971, 1278
Zsp2I ATGCAT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.