Rroxscaffold_1G00069520

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011534
Physical Location & Seq
Forward (+)
90456334 .. 90461159
4826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00069520.1

Sequence Viewer

Length: 1917 bp
ATGCAGACGAGGTACATGGAGAGATCGAATAGTATGGCGAGGGAGAAGCGAGGTCTGGATGCCGCCGCAGCTGAAGAAGGCCAGCCTGATAGGAAGCGGCCAGCTCTGGCAAGTGTAATTGTTGAAGCTCTCAAGGTGGACAGTCTGCAAAAGCTTTGCTCCTCACTGGAGCCAATTCTACGTAGAGTTGTCAGTGAAGAGGTAGAGCGTGCTTTAGCAAAATTAGGCCCTGCTAAACTTACTGGAAGCAGGTCATCTCCTCCTAAACAAATTGAAGGACCTGATGGAAGAAACCTGCAGCTGCACTTCCAGTCCAAGTTGTCTCTTCCTCTCTTTACTGGTGGAAAAGTAGAAGGAGAGTGCGGTGCCGCAATCCATATTGTGTTGATTGATGCAAATACAAACCGTGTTGTCACATCAGGTCCAGAGTCAATGGTGAAACTAGATGTTATTGTTCTTGAAGGTGATTTTAACAATGAGGACGATGATAACTGGACTGAAGAAGAATTTGAGAGCCATGTGGTGAAAGAGCGTGAAGGAAAGAGGCCACTTCTCACTGGGGATCTTCAAGTGACACTGAAAGAAGGTGTAGGAACACTAGGGGAGCTGACATTTACCGATAATTCAAGCTGGATTAGGAGCAGGAAGTTCAGGCTAGGATTGAAGGTTGCCTCAGGCTTTTGCGATGGGATTCGTATACGTGAAGCGAAAACAGATGCCTTCACCGTTAAGGACCACCGTGGGGAATTATACAAGAAACACTACCCACCTGTGCTAACTGATGAGGTTTGGAGATTGGAGAAAATTGGAAAAGATGGGTCATTTCACAAGAGACTGAATAAGGCCGGAGTCTTTACTGTTGAAGACTTCCTACGACTTGTGAATAGAGACTCGCAGAGACTGCGGAATATTCTTGGGAGTGGCATGTCGAATAAGATGTGGGACGTCCTTATACAACATGCAAAGACCTGTCTTCTAGGTGGTAAACTCTATGTGTACTATCCTGAGGATGCAAGGAGTGTTGGTGTTGTTTTCAACAATATCTATGAGTTGAGTGGGTTAATCACCAATGAACAGTATATCTCCGCTGACTGTCTCTCAGACAGCCAGAAGGTCTATGTCGACGGTTTGGTGAAGAAGGCATATGAAAACTGGATGCATGTTATGGAGTATGATGGAAAGTCTCTTCTAAACTTCAAGCAGCATAAGGGTCCAGTTACCTCTCAACTTGAGGTTCCCATGGCTTCGCAGGAGTATACAAACTCATTTAACCAGCAGTTCACGCTACCTAGCCTGCCAGTTCCTTCAGAGCAGAATGTTATGGATCCTGGATTTATTGCGGGAGGTTATAATGATGGCATGGCAACCCGATTTTCAATACCTCCACAGAACGTAAATCTCAATCCTCCTGTTCAGTTTGATGGCTCTTCATTCCCTCTACAGAGTCAGTTGCCCAGCACTTCGCACCCACATGAAATCCTAAGAAGTGAAAATATGCGTGCCGTTGGTCCGCCACAATCATCCACATCAGGATTCCAGAATGTGGATGCATCCAATCTTGCTTCTTATAGGGGACTTGACTTTTTCCCAGAGGAAGAAATTCGTATGAGGAGCCATGAGTTACTTGAAAATGAAGATATGCAGCATCTACTTCGTATGTTTAGCATGGGTGGTCATGGTCAAGTTCACGGTCAGGGTGTCCATCCTTCCATAAATGCTACCGAAGACTATTCTTACTCCTCAGCATATATGCCCAGTCCATCGATAAACTACAATTTCGACGATGATCGCAGCCGTTCGTCTGGTAAGGCTGTTGTTGGTTGGCTCAAACTTAAGGCAGCCCTTAGATGGGGAATCTTCATTAGAAAGAAGGCTGCTGAGAGACGGGCACAACTGGTTGAGTTGGATGAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

638

Amino Acids

71.44

Weight (kDa)

6.2

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 99 - 246 2.6e-63 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 259 - 324 2.4e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 329 - 391 2.9e-23 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1350
AatII GACGTC 1 cut(s) 948
Acc36I ACCTGC 2 cut(s) 240, 303
AccB1I GGYRCC 1 cut(s) 365
AccB7I CCANNNNNTGG 1 cut(s) 1543
AccI GTMKAC 3 cut(s) 697, 1122, 1256
AciI CCGC 9 cut(s) 63, 66, 97, 363, 369, 904, 1086, 1340, 1511
AclWI GGATC 3 cut(s) 570, 1319, 1332
AcoI YGGCCR 1 cut(s) 98
AcsI RAATTY 2 cut(s) 506, 1599
AcuI CTGAAG 3 cut(s) 93, 519, 1290
AcyI GRCGYC 1 cut(s) 945
AfaI GTAC 2 cut(s) 14, 998
AfiI CCNNNNNNNGG 4 cut(s) 742, 1543, 1848, 1849
AflII CTTAAG 1 cut(s) 1832
AjnI CCWGG 1 cut(s) 1327
AluBI AGCT 7 cut(s) 71, 104, 128, 154, 301, 607, 630
AluI AGCT 7 cut(s) 71, 104, 128, 154, 301, 607, 630
Alw26I GTCTC 7 cut(s) 327, 826, 882, 892, 1100, 1188, 1876
AlwI GGATC 3 cut(s) 570, 1319, 1332
AlwNI CAGNNNCTG 1 cut(s) 901
AoxI GGCC 5 cut(s) 79, 98, 226, 545, 843
ApeKI GCWGC 8 cut(s) 68, 298, 301, 1201, 1642, 1791, 1838, 1874
ApoI RAATTY 2 cut(s) 506, 1599
Asp700I GAANNNNTTC 1 cut(s) 1599
AspS9I GGNCC 6 cut(s) 227, 278, 422, 733, 1211, 1508
AsuHPI GGTGA 6 cut(s) 448, 476, 535, 715, 1057, 1144
AvaII GGWCC 5 cut(s) 278, 422, 733, 1211, 1508
AxyI CCTNAGG 2 cut(s) 673, 1005
BaeGI GKGCMC 1 cut(s) 1891
BamHI GGATCC 1 cut(s) 1324
BanI GGYRCC 1 cut(s) 365
BarI GAAGNNNNNNTAC 2 cut(s) 855, 887
BbsI GAAGAC 3 cut(s) 870, 965, 1731
BbvCI CCTCAGC 1 cut(s) 1741
BbvI GCAGC 8 cut(s) 80, 288, 310, 1213, 1654, 1803, 1850, 1861
BccI CCATC 9 cut(s) 278, 680, 809, 1169, 1349, 1415, 1710, 1768, 1842
BceAI ACGGC 2 cut(s) 1487, 1779
BcgI CGANNNNNNTGC 2 cut(s) 1634, 1668
BciT130I CCWGG 1 cut(s) 1329
BcoDI GTCTC 7 cut(s) 327, 826, 882, 892, 1100, 1188, 1876
BfaI CTAG 5 cut(s) 443, 599, 656, 977, 1290
BfmI CTRYAG 2 cut(s) 296, 1439
BfrI CTTAAG 1 cut(s) 1832
BfuAI ACCTGC 2 cut(s) 240, 303
Bme1390I CCNGG 1 cut(s) 1329
Bme18I GGWCC 5 cut(s) 278, 422, 733, 1211, 1508
BmgT120I GGNCC 6 cut(s) 227, 278, 422, 733, 1211, 1508
BmiI GGNNCC 6 cut(s) 171, 367, 1212, 1236, 1326, 1613
BmrFI CCNGG 1 cut(s) 1329
BmrI ACTGGG 2 cut(s) 567, 1749
BmsI GCATC 8 cut(s) 49, 382, 706, 1000, 1146, 1537, 1559, 1654
BmuI ACTGGG 2 cut(s) 567, 1749
BpiI GAAGAC 3 cut(s) 870, 965, 1731
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 1741
BpuEI CTTGAG 2 cut(s) 116, 1250
Bsa29I ATCGAT 1 cut(s) 1763
BsaAI YACGTR 2 cut(s) 182, 701
BsaHI GRCGYC 1 cut(s) 945
BsaJI CCNNGG 2 cut(s) 739, 1239
BsaXI ACNNNNNCTCC 2 cut(s) 1009, 1039
Bsc4I CCNNNNNNNGG 4 cut(s) 742, 1543, 1848, 1849
Bse21I CCTNAGG 2 cut(s) 673, 1005
BseBI CCWGG 1 cut(s) 1329
BseCI ATCGAT 1 cut(s) 1763
BseDI CCNNGG 2 cut(s) 739, 1239
BseGI GGATG 8 cut(s) 64, 1015, 1161, 1520, 1550, 1552, 1702, 1912
BseLI CCNNNNNNNGG 4 cut(s) 742, 1543, 1848, 1849
BseMII CTCAG 5 cut(s) 687, 996, 1113, 1755, 1869
BseRI GAGGAG 4 cut(s) 151, 249, 1624, 1729
BseSI GKGCMC 1 cut(s) 1891
BseXI GCAGC 8 cut(s) 80, 288, 310, 1213, 1654, 1803, 1850, 1861
BseYI CCCAGC 1 cut(s) 1454
BsgI GTGCAG 1 cut(s) 287
BshFI GGCC 5 cut(s) 81, 100, 228, 547, 845
BshNI GGYRCC 1 cut(s) 365
BshVI ATCGAT 1 cut(s) 1763
BsiSI CCGG 1 cut(s) 846
BslFI GGGAC 2 cut(s) 956, 1587
BslI CCNNNNNNNGG 4 cut(s) 742, 1543, 1848, 1849
BsmAI GTCTC 7 cut(s) 327, 826, 882, 892, 1100, 1188, 1876
BsmBI CGTCTC 1 cut(s) 1876
BsmFI GGGAC 2 cut(s) 956, 1587
BsnI GGCC 5 cut(s) 81, 100, 228, 547, 845
Bsp1286I GDGCHC 1 cut(s) 1891
Bsp143I GATC 4 cut(s) 23, 562, 1324, 1786
Bsp19I CCATGG 1 cut(s) 1239
BspACI CCGC 9 cut(s) 63, 66, 97, 363, 369, 904, 1086, 1340, 1511
BspANI GGCC 5 cut(s) 81, 100, 228, 547, 845
BspCNI CTCAG 5 cut(s) 686, 997, 1112, 1754, 1870
BspDI ATCGAT 1 cut(s) 1763
BspLI GGNNCC 6 cut(s) 171, 367, 1212, 1236, 1326, 1613
BspMAI CTGCAG 1 cut(s) 300
BspMI ACCTGC 2 cut(s) 240, 303
BspPI GGATC 3 cut(s) 570, 1319, 1332
BspQI GCTCTTC 1 cut(s) 1432
BspT107I GGYRCC 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 1832
BssECI CCNNGG 2 cut(s) 739, 1239
BssMI GATC 4 cut(s) 23, 562, 1324, 1786
BssNAI GTATAC 2 cut(s) 698, 1257
BssNI GRCGYC 1 cut(s) 945
BssT1I CCWWGG 1 cut(s) 1239
Bst1107I GTATAC 2 cut(s) 698, 1257
Bst2UI CCWGG 1 cut(s) 1329
Bst4CI ACNGT 9 cut(s) 143, 407, 727, 740, 859, 1077, 1094, 1127, 1691
Bst6I CTCTTC 4 cut(s) 192, 330, 1191, 1432
BstACI GRCGYC 1 cut(s) 945
BstAFI CTTAAG 1 cut(s) 1832
BstAPI GCANNNNNTGC 1 cut(s) 901
BstBAI YACGTR 2 cut(s) 182, 701
BstC8I GCNNGC 5 cut(s) 83, 102, 210, 1295, 1500
BstDEI CTNAG 7 cut(s) 673, 1005, 1099, 1481, 1741, 1844, 1878
BstDSI CCRYGG 2 cut(s) 739, 1239
BstF5I GGATG 8 cut(s) 64, 1015, 1161, 1520, 1550, 1552, 1702, 1912
BstKTI GATC 4 cut(s) 26, 565, 1327, 1789
BstMAI GTCTC 7 cut(s) 327, 826, 882, 892, 1100, 1188, 1876
BstMBI GATC 4 cut(s) 23, 562, 1324, 1786
BstMWI GCNNNNNNNGC 3 cut(s) 68, 901, 1282
BstNI CCWGG 1 cut(s) 1329
BstNSI RCATGY 3 cut(s) 928, 962, 1163
BstSCI CCNGG 1 cut(s) 1327
BstSFI CTRYAG 2 cut(s) 296, 1439
BstSLI GKGCMC 1 cut(s) 1891
BstSNI TACGTA 1 cut(s) 182
BstV1I GCAGC 8 cut(s) 80, 288, 310, 1213, 1654, 1803, 1850, 1861
BstV2I GAAGAC 3 cut(s) 870, 965, 1731
BstX2I RGATCY 2 cut(s) 562, 1324
BstYI RGATCY 2 cut(s) 562, 1324
BstZ17I GTATAC 2 cut(s) 698, 1257
Bsu15I ATCGAT 1 cut(s) 1763
Bsu36I CCTNAGG 2 cut(s) 673, 1005
BsuRI GGCC 5 cut(s) 81, 100, 228, 547, 845
BsuTUI ATCGAT 1 cut(s) 1763
BtgI CCRYGG 2 cut(s) 739, 1239
BtgZI GCGATG 1 cut(s) 699
BtsCI GGATG 8 cut(s) 64, 1015, 1161, 1520, 1550, 1552, 1702, 1912
BtsIMutI CAGTG 4 cut(s) 164, 199, 555, 575
BveI ACCTGC 2 cut(s) 240, 303
Cac8I GCNNGC 5 cut(s) 83, 102, 210, 1295, 1500
CaiI CAGNNNCTG 1 cut(s) 901
Cfr13I GGNCC 6 cut(s) 227, 278, 422, 733, 1211, 1508
ClaI ATCGAT 1 cut(s) 1763
Csp6I GTAC 2 cut(s) 13, 997
CviQI GTAC 2 cut(s) 13, 997
DdeI CTNAG 7 cut(s) 673, 1005, 1099, 1481, 1741, 1844, 1878
DpnI GATC 4 cut(s) 25, 564, 1326, 1788
DpnII GATC 4 cut(s) 23, 562, 1324, 1786
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 4 cut(s) 192, 330, 1191, 1432
EarI CTCTTC 4 cut(s) 192, 330, 1191, 1432
EciI GGCGGA 1 cut(s) 1500
Eco105I TACGTA 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 1239
Eco47I GGWCC 5 cut(s) 278, 422, 733, 1211, 1508
Eco57I CTGAAG 3 cut(s) 93, 519, 1290
Eco81I CCTNAGG 2 cut(s) 673, 1005
EcoO109I RGGNCCY 2 cut(s) 227, 278
EcoRII CCWGG 1 cut(s) 1327
EcoT14I CCWWGG 1 cut(s) 1239
EcoT22I ATGCAT 2 cut(s) 1161, 1552
ErhI CCWWGG 1 cut(s) 1239
Esp3I CGTCTC 1 cut(s) 1876
FaqI GGGAC 2 cut(s) 956, 1587
FauI CCCGC 1 cut(s) 1333
FauNDI CATATG 1 cut(s) 1144
FblI GTMKAC 3 cut(s) 697, 1122, 1256
FokI GGATG 7 cut(s) 71, 1022, 1168, 1507, 1537, 1559, 1689
FspBI CTAG 5 cut(s) 443, 599, 656, 977, 1290
GsaI CCCAGC 1 cut(s) 1458
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 5 cut(s) 81, 100, 228, 547, 845
HapII CCGG 1 cut(s) 846
Hin1I GRCGYC 1 cut(s) 945
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 8 cut(s) 428, 691, 849, 890, 1444, 1533, 1854, 1910
HpaII CCGG 1 cut(s) 846
HphI GGTGA 6 cut(s) 448, 476, 535, 715, 1057, 1144
Hpy166II GTNNAC 8 cut(s) 139, 698, 986, 997, 1123, 1257, 1281, 1687
Hpy188I TCNGA 2 cut(s) 1102, 1309
Hpy188III TCNNGA 6 cut(s) 56, 425, 458, 1004, 1530, 1537
Hpy8I GTNNAC 8 cut(s) 139, 698, 986, 997, 1123, 1257, 1281, 1687
Hpy99I CGWCG 2 cut(s) 1127, 1784
HpyCH4III ACNGT 9 cut(s) 143, 407, 727, 740, 859, 1077, 1094, 1127, 1691
HpyCH4IV ACGT 4 cut(s) 181, 700, 945, 1392
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 901, 1282
HpyF3I CTNAG 7 cut(s) 673, 1005, 1099, 1481, 1741, 1844, 1878
HpySE526I ACGT 4 cut(s) 181, 700, 945, 1392
Hsp92I GRCGYC 1 cut(s) 945
Kzo9I GATC 4 cut(s) 23, 562, 1324, 1786
LguI GCTCTTC 1 cut(s) 1432
LmnI GCTCC 5 cut(s) 164, 169, 604, 639, 1611
Lsp1109I GCAGC 8 cut(s) 80, 288, 310, 1213, 1654, 1803, 1850, 1861
LweI GCATC 8 cut(s) 49, 382, 706, 1000, 1146, 1537, 1559, 1654
MaeI CTAG 5 cut(s) 443, 599, 656, 977, 1290
MaeII ACGT 4 cut(s) 181, 700, 945, 1392
MaeIII GTNAC 4 cut(s) 412, 571, 1216, 1620
MalI GATC 4 cut(s) 25, 564, 1326, 1788
MboI GATC 4 cut(s) 23, 562, 1324, 1786
MflI RGATCY 2 cut(s) 562, 1324
MhlI GDGCHC 1 cut(s) 1891
MlyI GAGTC 4 cut(s) 437, 858, 884, 1453
MmeI TCCRAC 1 cut(s) 1884
Mph1103I ATGCAT 2 cut(s) 1161, 1552
MroXI GAANNNNTTC 1 cut(s) 1599
MseI TTAA 5 cut(s) 471, 729, 1061, 1269, 1833
MslI CAYNNNNRTG 1 cut(s) 1470
MspA1I CMGCKG 3 cut(s) 71, 301, 1088
MspCI CTTAAG 1 cut(s) 1832
MspI CCGG 1 cut(s) 846
MspR9I CCNGG 1 cut(s) 1329
MvaI CCWGG 1 cut(s) 1329
MwoI GCNNNNNNNGC 3 cut(s) 68, 901, 1282
NcoI CCATGG 1 cut(s) 1239
NdeI CATATG 1 cut(s) 1144
NdeII GATC 4 cut(s) 23, 562, 1324, 1786
NlaIV GGNNCC 6 cut(s) 171, 367, 1212, 1236, 1326, 1613
NmuCI GTSAC 2 cut(s) 412, 571
NsiI ATGCAT 2 cut(s) 1161, 1552
NspI RCATGY 3 cut(s) 928, 962, 1163
PciSI GCTCTTC 1 cut(s) 1432
PdmI GAANNNNTTC 1 cut(s) 1599
PfeI GAWTC 4 cut(s) 691, 1533, 1854, 1910
PflMI CCANNNNNTGG 1 cut(s) 1543
PfoI TCCNGGA 1 cut(s) 1327
PleI GAGTC 4 cut(s) 436, 857, 884, 1452
PpsI GAGTC 4 cut(s) 436, 857, 884, 1452
Ppu21I YACGTR 2 cut(s) 182, 701
PpuMI RGGWCCY 1 cut(s) 278
PsiI TTATAA 1 cut(s) 1350
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 1 cut(s) 1327
PspFI CCCAGC 1 cut(s) 1454
PspGI CCWGG 1 cut(s) 1327
PspN4I GGNNCC 6 cut(s) 171, 367, 1212, 1236, 1326, 1613
PspPI GGNCC 6 cut(s) 227, 278, 422, 733, 1211, 1508
PspPPI RGGWCCY 1 cut(s) 278
PstI CTGCAG 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 901
PsuI RGATCY 2 cut(s) 562, 1324
PvuII CAGCTG 2 cut(s) 71, 301
RsaI GTAC 2 cut(s) 14, 998
RsaNI GTAC 2 cut(s) 13, 997
RseI CAYNNNNRTG 1 cut(s) 1470
SalI GTCGAC 1 cut(s) 1121
SapI GCTCTTC 1 cut(s) 1432
SaqAI TTAA 5 cut(s) 471, 729, 1061, 1269, 1833
Sau3AI GATC 4 cut(s) 23, 562, 1324, 1786
Sau96I GGNCC 6 cut(s) 227, 278, 422, 733, 1211, 1508
SchI GAGTC 4 cut(s) 437, 858, 884, 1453
ScrFI CCNGG 1 cut(s) 1329
SduI GDGCHC 1 cut(s) 1891
SfaNI GCATC 8 cut(s) 49, 382, 706, 1000, 1146, 1537, 1559, 1654
SfcI CTRYAG 2 cut(s) 296, 1439
SinI GGWCC 5 cut(s) 278, 422, 733, 1211, 1508
SmiMI CAYNNNNRTG 1 cut(s) 1470
SmlI CTYRAG 3 cut(s) 131, 1229, 1832
SmoI CTYRAG 3 cut(s) 131, 1229, 1832
SnaBI TACGTA 1 cut(s) 182
SsiI CCGC 9 cut(s) 63, 66, 97, 363, 369, 904, 1086, 1340, 1511
SspI AATATT 1 cut(s) 910
SspMI CTAG 5 cut(s) 443, 599, 656, 977, 1290
StyD4I CCNGG 1 cut(s) 1327
StyI CCWWGG 1 cut(s) 1239
TaaI ACNGT 9 cut(s) 143, 407, 727, 740, 859, 1077, 1094, 1127, 1691
TaiI ACGT 4 cut(s) 184, 703, 948, 1395
TaqI TCGA 5 cut(s) 26, 929, 1122, 1763, 1779
TatI WGTACW 1 cut(s) 996
TauI GCSGC 4 cut(s) 65, 68, 100, 371
TfiI GAWTC 4 cut(s) 691, 1533, 1854, 1910
Tru1I TTAA 5 cut(s) 471, 729, 1061, 1269, 1833
Tru9I TTAA 5 cut(s) 471, 729, 1061, 1269, 1833
TscAI CASTG 4 cut(s) 171, 199, 562, 582
TseFI GTSAC 2 cut(s) 412, 571
TseI GCWGC 8 cut(s) 68, 298, 301, 1201, 1642, 1791, 1838, 1874
Tsp45I GTSAC 2 cut(s) 412, 571
TspDTI ATGAA 6 cut(s) 1086, 1161, 1419, 1488, 1647, 1849
TspRI CASTG 4 cut(s) 171, 199, 562, 582
Van91I CCANNNNNTGG 1 cut(s) 1543
Vha464I CTTAAG 1 cut(s) 1832
VpaK11BI GGWCC 5 cut(s) 278, 422, 733, 1211, 1508
XapI RAATTY 2 cut(s) 506, 1599
XceI RCATGY 3 cut(s) 928, 962, 1163
XmiI GTMKAC 3 cut(s) 697, 1122, 1256
XmnI GAANNNNTTC 1 cut(s) 1599
XspI CTAG 5 cut(s) 443, 599, 656, 977, 1290
ZraI GACGTC 1 cut(s) 946
Zsp2I ATGCAT 2 cut(s) 1161, 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.