MD10G1302900.v1.1

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011534
Physical Location & Seq
Forward (+)
38945122 .. 38948920
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1302900.v1.1.491

Sequence Viewer

Length: 1911 bp
ATGCAGACGAGGTACATGGAGAGATCCAACAGTATGGCGAGGGAGAAGCGAAGTTTGGATTCGTCTTCAGCTGAAGAAGGCCAGCCGGATAGGAAACGTCCTGCTCTCGCCAGTGTAATCGTTGAAGCTCTCAAGGTGGATAGTCTGCAAAAGCTTTGCTCGTCACTGGAGCCGATTCTGCGTAGAGTTGTTAGCGAAGAGGTGGAGCGTGCTTTAGCAAAATTAGGCCCTGCCAAACTTACTGGAAGGTCTTCTCCTAAACAAATTGAAGGGCCCAATGGAAGAAACTTGCAGCTGCATTTTATATCCAAGTTGTCCCTTCCTCTTTTTACTGGCGGAAAAGTAGAAGGGGAGTGGGGTTCTGCAATCTCTATTGTCTTGATTGATGCAAATACAAAACACGTCGTGACATCAGGTCCGGAGTCTGCGGTGAAATTAGACATAGTTGTGCTTGAAGGTGATTTTAACAACGAGGATGATGAGAACTGGACTCAAGAAGAATTTGAGAGCCATGTGGTGAAAGAGCGTGAAGGAAAGAGGCCACTTCTTACTGGGGATGTGCAAGTTACACTGAAGGAAGGTGTAGGAACACTAGGGGAATTGACATTTACCGATAACTCTAGCTGGATTAGGAGCAGGAAGTTTAGGCTAGGACTGAAAGTTGCTTCAGGCAATTGCGACAACATTCGTATACGGGAAGCAAAAACAGAAGCCATCACTGTTAAGGATCACCGTGGGGAATTGTACAAGAAACACTACCCACCTTTGTTACATGATGAGGTCTGGAGATTAGAGAAAATTGGAAAAGATGGATCATTCCACAAGAGGCTGAATAAAGCTGGAATTGTTACAGTTGAAGACTTCCTACGACTTGTGGTTAGAGACTCGCAGAGACTGCGGAATATTCTTGGAAGTGGCATGTCAAATAAGATGTGGGATGGACTCATACAGCATGCAAAAACTTGTCTTCTTGGTGGGAAACACTATGTCTATTATCTTGAGGATGCAAGGAATGTTGGTGTTGTTTTTAACAATATCTACGAGTTGAGTGGCCTAATTACCGATGAACAATTTTTCTCTGCTGATTCTCTCTCGGAAACTCAGAAGGTCTGCGTGGACGGTTTGGTAAAAAAGGCATATGACAACTGGATGCATGTTATGGAGTATGATGGAAAATCTCTTCTAAGCCAGAAAAATCCAGATGTTTCTCTACCTGAGGTCCCAATGGCCTCGCAAGATTATCCAAACTCATGTGATCAGCAGTTCACCCTACCTAGCCTGCCAGCTTCAGTTTCTTCACAACCGCCTCCTATGGATTCCGGCCTAAACGTGGGAGGTTATAGTGATGGCATGGCTGCCAGATTCTCAATACAGTCACAGAATGGAAATCTGAATGCTCCAATTCAGCTCGATGGCTTGTCATTTCCTCAACAGAATCAGCTGCCTAGCATTTCGCACCAGTCGCATTTTCAAAGAAATGAAAACATGCTTTCCCTTGGTCCACTACAGTCGTCCACATCAGGGTTCCAGAATATCGGCACATCCAATCTTTCTTCTTATAGGGGAGTCGAGGACCTCTTCCCAGAGGAAGAAATTCGTATGAGGAGCAATGAGATTCTCGAAAATGAAGATATGCAGCATCTGCTTCGTGTCATTAACATGGGCGGTCAGGGTCAAGGCCACAGTTATGGTCATTCCTCCATGAACGTCACTGAAGACAATTATCCTTATTCGTCACCATACATCCCCACTCCACAAGCGAACTACAGTATTGATGATGATCAGAGCCGTTCACCTGGGAAAGCTGTTGTTGGCTGGCTCAAGCTCAAGGCAGCGCTTAGATGGGGCATATTCGTCAGAAAGAAGGCTGCTGAGAGACGGGCTCAGCTTGTTGAGTTGGATGATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Calmodulin_bind

Protein Analysis

637

Amino Acids

70.97

Weight (kDa)

5.99

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 97 - 244 4.8e-63 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 257 - 322 1.5e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 327 - 389 2.8e-21 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 691
AccIII TCCGGA 1 cut(s) 418
AciI CCGC 5 cut(s) 336, 428, 898, 1304, 1665
AclWI GGATC 3 cut(s) 18, 735, 820
AcsI RAATTY 2 cut(s) 500, 1593
AcuI CTGAAG 6 cut(s) 51, 93, 593, 651, 1272, 1734
AdeI CACNNNGTG 1 cut(s) 406
AfaI GTAC 2 cut(s) 14, 746
AfeI AGCGCT 1 cut(s) 1836
AfiI CCNNNNNNNGG 2 cut(s) 1330, 1521
AflIII ACRYGT 1 cut(s) 400
AgsI TTSAA 5 cut(s) 125, 269, 455, 857, 1472
AhdI GACNNNNNGTC 1 cut(s) 414
AjiI CACGTC 1 cut(s) 403
AjnI CCWGG 1 cut(s) 1795
AjuI GAANNNNNNNTTGG 2 cut(s) 38, 70
Alw26I GTCTC 3 cut(s) 876, 886, 1870
AlwI GGATC 3 cut(s) 18, 735, 820
AlwNI CAGNNNCTG 2 cut(s) 895, 1642
Aor13HI TCCGGA 1 cut(s) 418
Aor51HI AGCGCT 1 cut(s) 1836
AoxI GGCC 8 cut(s) 79, 226, 272, 539, 1051, 1227, 1321, 1678
ApaI GGGCCC 1 cut(s) 276
ApeKI GCWGC 7 cut(s) 292, 295, 1355, 1441, 1636, 1832, 1868
ApoI RAATTY 2 cut(s) 500, 1593
Asp700I GAANNNNTTC 2 cut(s) 250, 1593
AspLEI GCGC 1 cut(s) 1837
AspS9I GGNCC 7 cut(s) 227, 272, 273, 416, 1219, 1499, 1573
AsuHPI GGTGA 7 cut(s) 442, 470, 529, 722, 1258, 1728, 1785
AvaII GGWCC 4 cut(s) 416, 1219, 1499, 1573
AxyI CCTNAGG 1 cut(s) 1215
BaeGI GKGCMC 1 cut(s) 276
BanII GRGCYC 2 cut(s) 276, 1885
BarI GAAGNNNNNNTAC 2 cut(s) 849, 881
BbsI GAAGAC 5 cut(s) 57, 243, 864, 959, 1722
BbvI GCAGC 7 cut(s) 282, 304, 1342, 1428, 1648, 1844, 1855
BccI CCATC 7 cut(s) 722, 803, 932, 1163, 1340, 1406, 1836
BceAI ACGGC 1 cut(s) 1773
BcgI CGANNNNNNTGC 2 cut(s) 1628, 1662
BciT130I CCWGG 1 cut(s) 1797
BclI TGATCA 2 cut(s) 1255, 1780
BcoDI GTCTC 3 cut(s) 876, 886, 1870
BfaI CTAG 5 cut(s) 593, 621, 650, 1275, 1446
BfmI CTRYAG 2 cut(s) 1505, 1765
BfoI RGCGCY 1 cut(s) 1838
BisI GCNGC 7 cut(s) 293, 296, 1356, 1442, 1637, 1833, 1869
BlpI GCTNAGC 1 cut(s) 1884
BlsI GCNGC 7 cut(s) 294, 297, 1357, 1443, 1638, 1834, 1870
Bme1390I CCNGG 1 cut(s) 1797
Bme18I GGWCC 4 cut(s) 416, 1219, 1499, 1573
BmeRI GACNNNNNGTC 1 cut(s) 414
BmgBI CACGTC 1 cut(s) 403
BmgT120I GGNCC 7 cut(s) 227, 272, 273, 416, 1219, 1499, 1573
BmiI GGNNCC 4 cut(s) 171, 274, 1221, 1526
BmrFI CCNGG 1 cut(s) 1797
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 4 cut(s) 376, 994, 1140, 1648
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 5 cut(s) 57, 243, 864, 959, 1722
BplI GAGNNNNNCTC 2 cut(s) 1867, 1899
BpmI CTGGAG 2 cut(s) 188, 805
Bpu1102I GCTNAGC 1 cut(s) 1884
BpuEI CTTGAG 5 cut(s) 116, 477, 1019, 1805, 1811
BsaBI GATNNNNATC 1 cut(s) 1779
BsaJI CCNNGG 3 cut(s) 733, 1495, 1796
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 1330, 1521
Bse1I ACTGG 8 cut(s) 111, 171, 247, 337, 491, 556, 1151, 1459
Bse21I CCTNAGG 1 cut(s) 1215
Bse3DI GCAATG 1 cut(s) 1615
Bse8I GATNNNNATC 1 cut(s) 1779
BseAI TCCGGA 1 cut(s) 418
BseBI CCWGG 1 cut(s) 1797
BseDI CCNNGG 3 cut(s) 733, 1495, 1796
BseGI GGATG 8 cut(s) 481, 562, 943, 1009, 1155, 1541, 1743, 1906
BseJI GATNNNNATC 1 cut(s) 1779
BseLI CCNNNNNNNGG 2 cut(s) 1330, 1521
BseMI GCAATG 1 cut(s) 1615
BseMII CTCAG 4 cut(s) 1115, 1206, 1863, 1898
BseNI ACTGG 8 cut(s) 111, 171, 247, 337, 491, 556, 1151, 1459
BseRI GAGGAG 1 cut(s) 1618
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 7 cut(s) 282, 304, 1342, 1428, 1648, 1844, 1855
BshFI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1229, 1323, 1680
BsiSI CCGG 3 cut(s) 86, 419, 1320
BslFI GGGAC 2 cut(s) 301, 1205
BslI CCNNNNNNNGG 2 cut(s) 1330, 1521
BsmAI GTCTC 3 cut(s) 876, 886, 1870
BsmBI CGTCTC 1 cut(s) 1870
BsmFI GGGAC 2 cut(s) 301, 1205
BsmI GAATGC 1 cut(s) 1399
BsnI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1229, 1323, 1680
Bsp120I GGGCCC 1 cut(s) 272
Bsp1286I GDGCHC 2 cut(s) 276, 1885
Bsp13I TCCGGA 1 cut(s) 418
Bsp1407I TGTACA 1 cut(s) 744
Bsp143I GATC 5 cut(s) 23, 727, 812, 1255, 1780
Bsp1720I GCTNAGC 1 cut(s) 1884
BspACI CCGC 5 cut(s) 336, 428, 898, 1304, 1665
BspANI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1229, 1323, 1680
BspCNI CTCAG 4 cut(s) 1114, 1207, 1864, 1897
BspEI TCCGGA 1 cut(s) 418
BspLI GGNNCC 4 cut(s) 171, 274, 1221, 1526
BspPI GGATC 3 cut(s) 18, 735, 820
BsrDI GCAATG 1 cut(s) 1615
BsrGI TGTACA 1 cut(s) 744
BsrI ACTGG 8 cut(s) 111, 171, 247, 337, 491, 556, 1151, 1459
BssECI CCNNGG 3 cut(s) 733, 1495, 1796
BssMI GATC 5 cut(s) 23, 727, 812, 1255, 1780
BssNAI GTATAC 1 cut(s) 692
BssT1I CCWWGG 1 cut(s) 1495
Bst1107I GTATAC 1 cut(s) 692
Bst2UI CCWGG 1 cut(s) 1797
Bst4CI ACNGT 9 cut(s) 32, 721, 734, 853, 1121, 1374, 1509, 1685, 1769
Bst6I CTCTTC 3 cut(s) 192, 1185, 1583
BstAPI GCANNNNNTGC 2 cut(s) 895, 1642
BstAUI TGTACA 1 cut(s) 744
BstC8I GCNNGC 6 cut(s) 83, 210, 954, 1280, 1284, 1817
BstDEI CTNAG 6 cut(s) 1101, 1184, 1215, 1838, 1872, 1884
BstDSI CCRYGG 1 cut(s) 733
BstF5I GGATG 8 cut(s) 481, 562, 943, 1009, 1155, 1541, 1743, 1906
BstH2I RGCGCY 1 cut(s) 1838
BstHHI GCGC 1 cut(s) 1837
BstKTI GATC 5 cut(s) 26, 730, 815, 1258, 1783
BstMAI GTCTC 3 cut(s) 876, 886, 1870
BstMBI GATC 5 cut(s) 23, 727, 812, 1255, 1780
BstMWI GCNNNNNNNGC 4 cut(s) 178, 895, 1462, 1642
BstNI CCWGG 1 cut(s) 1797
BstNSI RCATGY 4 cut(s) 922, 956, 1157, 1489
BstSCI CCNGG 1 cut(s) 1795
BstSFI CTRYAG 2 cut(s) 1505, 1765
BstSLI GKGCMC 1 cut(s) 276
BstV1I GCAGC 7 cut(s) 282, 304, 1342, 1428, 1648, 1844, 1855
BstV2I GAAGAC 5 cut(s) 57, 243, 864, 959, 1722
BstX2I RGATCY 1 cut(s) 23
BstXI CCANNNNNNTGG 2 cut(s) 34, 1688
BstYI RGATCY 1 cut(s) 23
BstZ17I GTATAC 1 cut(s) 692
Bsu36I CCTNAGG 1 cut(s) 1215
BsuRI GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1229, 1323, 1680
BtgI CCRYGG 1 cut(s) 733
BtrI CACGTC 1 cut(s) 403
BtsCI GGATG 8 cut(s) 481, 562, 943, 1009, 1155, 1541, 1743, 1906
BtsIMutI CAGTG 5 cut(s) 118, 164, 569, 717, 1710
Cac8I GCNNGC 6 cut(s) 83, 210, 954, 1280, 1284, 1817
CaiI CAGNNNCTG 2 cut(s) 895, 1642
CfoI GCGC 1 cut(s) 1837
Cfr13I GGNCC 7 cut(s) 227, 272, 273, 416, 1219, 1499, 1573
Csp6I GTAC 2 cut(s) 13, 745
CviQI GTAC 2 cut(s) 13, 745
DdeI CTNAG 6 cut(s) 1101, 1184, 1215, 1838, 1872, 1884
DpnI GATC 5 cut(s) 25, 729, 814, 1257, 1782
DpnII GATC 5 cut(s) 23, 727, 812, 1255, 1780
DraIII CACNNNGTG 1 cut(s) 406
DriI GACNNNNNGTC 1 cut(s) 414
Eam1104I CTCTTC 3 cut(s) 192, 1185, 1583
Eam1105I GACNNNNNGTC 1 cut(s) 414
EarI CTCTTC 3 cut(s) 192, 1185, 1583
EciI GGCGGA 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 1495
Eco24I GRGCYC 2 cut(s) 276, 1885
Eco47I GGWCC 4 cut(s) 416, 1219, 1499, 1573
Eco47III AGCGCT 1 cut(s) 1836
Eco57I CTGAAG 6 cut(s) 51, 93, 593, 651, 1272, 1734
Eco81I CCTNAGG 1 cut(s) 1215
EcoO109I RGGNCCY 4 cut(s) 227, 272, 1219, 1573
EcoRII CCWGG 1 cut(s) 1795
EcoT14I CCWWGG 1 cut(s) 1495
EcoT22I ATGCAT 1 cut(s) 1155
EcoT38I GRGCYC 2 cut(s) 276, 1885
ErhI CCWWGG 1 cut(s) 1495
Esp3I CGTCTC 1 cut(s) 1870
FaqI GGGAC 2 cut(s) 301, 1205
FauNDI CATATG 1 cut(s) 1138
FbaI TGATCA 2 cut(s) 1255, 1780
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 7 cut(s) 293, 296, 1356, 1442, 1637, 1833, 1869
FokI GGATG 7 cut(s) 488, 569, 950, 1016, 1162, 1528, 1730
FriOI GRGCYC 2 cut(s) 276, 1885
Fsp4HI GCNGC 7 cut(s) 293, 296, 1356, 1442, 1637, 1833, 1869
FspBI CTAG 5 cut(s) 593, 621, 650, 1275, 1446
GlaI GCGC 1 cut(s) 1836
GluI GCNGC 7 cut(s) 293, 296, 1356, 1442, 1637, 1833, 1869
GsuI CTGGAG 2 cut(s) 188, 805
HaeII RGCGCY 1 cut(s) 1838
HaeIII GGCC 8 cut(s) 81, 228, 274, 541, 1053, 1229, 1323, 1680
HapII CCGG 3 cut(s) 86, 419, 1320
HhaI GCGC 1 cut(s) 1837
Hin6I GCGC 1 cut(s) 1835
HinP1I GCGC 1 cut(s) 1835
HindIII AAGCTT 1 cut(s) 152
HpaII CCGG 3 cut(s) 86, 419, 1320
HphI GGTGA 7 cut(s) 442, 470, 529, 722, 1258, 1728, 1785
Hpy166II GTNNAC 6 cut(s) 692, 1117, 1266, 1502, 1515, 1793
Hpy188I TCNGA 5 cut(s) 1096, 1104, 1392, 1785, 1859
Hpy188III TCNNGA 9 cut(s) 379, 406, 419, 494, 784, 998, 1199, 1528, 1619
Hpy8I GTNNAC 6 cut(s) 692, 1117, 1266, 1502, 1515, 1793
Hpy99I CGWCG 1 cut(s) 407
HpyCH4III ACNGT 9 cut(s) 32, 721, 734, 853, 1121, 1374, 1509, 1685, 1769
HpyCH4IV ACGT 4 cut(s) 97, 402, 1329, 1707
HpyF10VI GCNNNNNNNGC 4 cut(s) 178, 895, 1462, 1642
HpyF3I CTNAG 6 cut(s) 1101, 1184, 1215, 1838, 1872, 1884
HpySE526I ACGT 4 cut(s) 97, 402, 1329, 1707
HspAI GCGC 1 cut(s) 1835
Kpn2I TCCGGA 1 cut(s) 418
Ksp22I TGATCA 2 cut(s) 1255, 1780
Kzo9I GATC 5 cut(s) 23, 727, 812, 1255, 1780
LmnI GCTCC 5 cut(s) 169, 205, 633, 1402, 1605
Lsp1109I GCAGC 7 cut(s) 282, 304, 1342, 1428, 1648, 1844, 1855
LweI GCATC 4 cut(s) 376, 994, 1140, 1648
MaeI CTAG 5 cut(s) 593, 621, 650, 1275, 1446
MaeII ACGT 4 cut(s) 97, 402, 1329, 1707
MaeIII GTNAC 8 cut(s) 162, 406, 565, 768, 847, 1374, 1708, 1734
MalI GATC 5 cut(s) 25, 729, 814, 1257, 1782
MboI GATC 5 cut(s) 23, 727, 812, 1255, 1780
MfeI CAATTG 1 cut(s) 673
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 2 cut(s) 276, 1885
MlyI GAGTC 5 cut(s) 431, 484, 878, 936, 1575
MmeI TCCRAC 2 cut(s) 51, 1878
Mph1103I ATGCAT 1 cut(s) 1155
MroI TCCGGA 1 cut(s) 418
MroXI GAANNNNTTC 2 cut(s) 250, 1593
MseI TTAA 4 cut(s) 465, 723, 1029, 1656
MslI CAYNNNNRTG 3 cut(s) 446, 1658, 1686
MspA1I CMGCKG 3 cut(s) 71, 295, 1441
MspI CCGG 3 cut(s) 86, 419, 1320
MspR9I CCNGG 1 cut(s) 1797
MunI CAATTG 1 cut(s) 673
Mva1269I GAATGC 1 cut(s) 1399
MvaI CCWGG 1 cut(s) 1797
MwoI GCNNNNNNNGC 4 cut(s) 178, 895, 1462, 1642
NdeI CATATG 1 cut(s) 1138
NdeII GATC 5 cut(s) 23, 727, 812, 1255, 1780
NlaIV GGNNCC 4 cut(s) 171, 274, 1221, 1526
NmuCI GTSAC 5 cut(s) 162, 406, 1374, 1708, 1734
NsiI ATGCAT 1 cut(s) 1155
NspI RCATGY 4 cut(s) 922, 956, 1157, 1489
PaeI GCATGC 1 cut(s) 956
PctI GAATGC 1 cut(s) 1399
PdmI GAANNNNTTC 2 cut(s) 250, 1593
PfeI GAWTC 7 cut(s) 59, 175, 1085, 1316, 1362, 1435, 1615
PkrI GCNGC 7 cut(s) 294, 297, 1357, 1443, 1638, 1834, 1870
PleI GAGTC 5 cut(s) 430, 484, 878, 936, 1574
PpsI GAGTC 5 cut(s) 430, 484, 878, 936, 1574
PpuMI RGGWCCY 2 cut(s) 1219, 1573
Psp5II RGGWCCY 2 cut(s) 1219, 1573
Psp6I CCWGG 1 cut(s) 1795
PspGI CCWGG 1 cut(s) 1795
PspN4I GGNNCC 4 cut(s) 171, 274, 1221, 1526
PspOMI GGGCCC 1 cut(s) 272
PspPI GGNCC 7 cut(s) 227, 272, 273, 416, 1219, 1499, 1573
PspPPI RGGWCCY 2 cut(s) 1219, 1573
PstNI CAGNNNCTG 2 cut(s) 895, 1642
PsuI RGATCY 1 cut(s) 23
PvuII CAGCTG 3 cut(s) 71, 295, 1441
RsaI GTAC 2 cut(s) 14, 746
RsaNI GTAC 2 cut(s) 13, 745
RseI CAYNNNNRTG 3 cut(s) 446, 1658, 1686
SaqAI TTAA 4 cut(s) 465, 723, 1029, 1656
SatI GCNGC 7 cut(s) 293, 296, 1356, 1442, 1637, 1833, 1869
Sau3AI GATC 5 cut(s) 23, 727, 812, 1255, 1780
Sau96I GGNCC 7 cut(s) 227, 272, 273, 416, 1219, 1499, 1573
SchI GAGTC 5 cut(s) 431, 484, 878, 936, 1575
ScrFI CCNGG 1 cut(s) 1797
SduI GDGCHC 2 cut(s) 276, 1885
SfaNI GCATC 4 cut(s) 376, 994, 1140, 1648
SfcI CTRYAG 2 cut(s) 1505, 1765
SinI GGWCC 4 cut(s) 416, 1219, 1499, 1573
SmiMI CAYNNNNRTG 3 cut(s) 446, 1658, 1686
SmlI CTYRAG 5 cut(s) 131, 492, 998, 1820, 1826
SmoI CTYRAG 5 cut(s) 131, 492, 998, 1820, 1826
SphI GCATGC 1 cut(s) 956
SsiI CCGC 5 cut(s) 336, 428, 898, 1304, 1665
SspI AATATT 1 cut(s) 904
SspMI CTAG 5 cut(s) 593, 621, 650, 1275, 1446
StyD4I CCNGG 1 cut(s) 1795
StyI CCWWGG 1 cut(s) 1495
TaaI ACNGT 9 cut(s) 32, 721, 734, 853, 1121, 1374, 1509, 1685, 1769
TaiI ACGT 4 cut(s) 100, 405, 1332, 1710
TaqI TCGA 3 cut(s) 1410, 1569, 1620
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 7 cut(s) 59, 175, 1085, 1316, 1362, 1435, 1615
Tru1I TTAA 4 cut(s) 465, 723, 1029, 1656
Tru9I TTAA 4 cut(s) 465, 723, 1029, 1656
TscAI CASTG 5 cut(s) 118, 171, 576, 724, 1717
TseFI GTSAC 5 cut(s) 162, 406, 1374, 1708, 1734
TseI GCWGC 7 cut(s) 292, 295, 1355, 1441, 1636, 1832, 1868
Tsp45I GTSAC 5 cut(s) 162, 406, 1374, 1708, 1734
TspDTI ATGAA 4 cut(s) 1080, 1494, 1641, 1718
TspRI CASTG 5 cut(s) 118, 171, 576, 724, 1717
VpaK11BI GGWCC 4 cut(s) 416, 1219, 1499, 1573
XapI RAATTY 2 cut(s) 500, 1593
XceI RCATGY 4 cut(s) 922, 956, 1157, 1489
XmiI GTMKAC 1 cut(s) 691
XmnI GAANNNNTTC 2 cut(s) 250, 1593
XspI CTAG 5 cut(s) 593, 621, 650, 1275, 1446
Zsp2I ATGCAT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.