MD05G1350700.v1.1

Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012902
Physical Location & Seq
Forward (+)
46804707 .. 46806786
2080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1350700.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGTTCAAATCTCCGACGTCGTAGCGTCTTCGTTTTCAATCCGCACCTTCTGGCGCTCCAGTCAGCAATGGCAACCGCGAGTCTTTAAGCTTCCACGCCAGATGCAATGGCAGTGTAATCGCAATTCGCAACCCGACAATCTGGAGAAGGCGGAGAGAGAAAACGAACCCGAAGGAGAGGAGCAACCAGGCATGGCCAGCTCCGCCGCAATCGACGGTGTCGCAGCGGCGGCGCTCCGCTCGGTGCTGCAGCGCATCCAGAAGGCAGCCGAAAAATCAGCGCGCAAAGCCCAAGACATCCGAGTGGTGGCGGTGAGCAAGACGAAACCAGTCTCCCTGCTCCGCCAAGTGTACGAGGCCGGCCATCGCTGTTTCGGCGAAAACTACGTCCAAGAAATCGTCGAGAAAGCTCCTCAGCTTCCGGAGGACATTGAGTGGCATTTCATTGGGAATTTGCAGAGCAATAAAGTAAAACCCCTTCTAACCGGAGTACCAAACCTTGCAATGGTAGAGAGTGTGGACGATGAGAAGATTGCTAATCGTCTTGATCACGTGGTTGGCAGCATCGGGAGAAAGCCTTTGAAGGTCTTAATCCAAGTGAACACCAGTGGAGAAGAATCAAAATTTGGTGTTGAGCCCTCTGGATGTGTGGAGCTCGCAAAGCATGTAACTTCGGGTTGTCCATACCTTGAGTTTTGTGGTCTAATGACAATTGGGATGCTAGATTATACTTCTACACCGGAAAACTTTAAGACGTTGGCGAACTGCAGAACTGAGGTTTGCAAGGCACTCGGAATACCAGAGGAGCAATGTGAGCTTTCAATGGGCATGTCTTCGGATTTTGAGCTAGCGATCGAAATGGGGAGCACAAACGTGAGAATTGGATCGACAATATTCGGGCCGAGAGAATATCCAAAGAAACAATCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ala_racemase_N

Protein Analysis

311

Amino Acids

34.42

Weight (kDa)

5.95

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ala_racemase_N PF01168 83 - 301 2.5e-15 Alanine racemase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11930 AT1G11930
fragaria_vesca FvH4_3g01630 FvH4_3g01630 FvH4_3g01630
malus_domestica MD05G1350700.v1.1 MD10G1325400.v1.1
prunus_persica Prupe.4G016700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002421
rosa_laevigata RLG00000031031
rosa_multiflora Rmu_sc0001717.1_g000005
rosa_roxburghii Rroxscaffold_1G00073430
rosa_rugosa Rorug04G0396100
rosa_samantha Rh5AG019000 Rh5BG021900 Rh5CG020900 Rh5DG020000
rosa_wichuraiana Rw5G001780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 21
AccBSI CCGCTC 1 cut(s) 240
AccII CGCG 2 cut(s) 79, 283
AccIII TCCGGA 1 cut(s) 421
AclWI GGATC 1 cut(s) 892
AcoI YGGCCR 2 cut(s) 195, 361
AcsI RAATTY 2 cut(s) 451, 623
AcvI CACGTG 1 cut(s) 553
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 2 cut(s) 353, 492
AfiI CCNNNNNNNGG 2 cut(s) 307, 505
AgsI TTSAA 4 cut(s) 8, 39, 583, 822
AjnI CCWGG 1 cut(s) 187
AjuI GAANNNNNNNTTGG 2 cut(s) 609, 641
AleI CACNNNNGTG 1 cut(s) 872
AluBI AGCT 7 cut(s) 91, 201, 410, 418, 655, 817, 847
AluI AGCT 7 cut(s) 91, 201, 410, 418, 655, 817, 847
Alw21I GWGCWC 2 cut(s) 657, 869
Alw26I GTCTC 1 cut(s) 337
AlwI GGATC 1 cut(s) 892
Aor13HI TCCGGA 1 cut(s) 421
AoxI GGCC 4 cut(s) 195, 357, 361, 899
ApeKI GCWGC 5 cut(s) 224, 247, 250, 266, 561
ApoI RAATTY 2 cut(s) 451, 623
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
AspLEI GCGC 5 cut(s) 57, 235, 255, 283, 285
AspS9I GGNCC 1 cut(s) 899
AsuHPI GGTGA 1 cut(s) 325
AsuNHI GCTAGC 1 cut(s) 847
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BalI TGGCCA 1 cut(s) 197
BanII GRGCYC 2 cut(s) 639, 657
BarI GAAGNNNNNNTAC 2 cut(s) 462, 494
BbrPI CACGTG 1 cut(s) 553
BbsI GAAGAC 2 cut(s) 21, 825
Bbv12I GWGCWC 2 cut(s) 657, 869
BbvCI CCTCAGC 1 cut(s) 414
BbvI GCAGC 5 cut(s) 234, 236, 262, 278, 573
BccI CCATC 1 cut(s) 372
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 772, 806
BciT130I CCWGG 1 cut(s) 189
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 2 cut(s) 722, 848
BfmI CTRYAG 2 cut(s) 248, 766
BfoI RGCGCY 2 cut(s) 58, 236
BisI GCNGC 8 cut(s) 207, 225, 228, 231, 248, 251, 267, 562
BlsI GCNGC 8 cut(s) 208, 226, 229, 232, 249, 252, 268, 563
Bme1390I CCNGG 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 899
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 4 cut(s) 93, 264, 573, 708
BmtI GCTAGC 1 cut(s) 851
BpiI GAAGAC 2 cut(s) 21, 825
BpmI CTGGAG 2 cut(s) 43, 164
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 710
BsaAI YACGTR 1 cut(s) 553
BsaHI GRCGYC 1 cut(s) 18
BsaWI WCCGGW 3 cut(s) 421, 485, 739
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 505
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 60, 329, 606
Bse3DI GCAATG 4 cut(s) 74, 113, 510, 815
BseAI TCCGGA 1 cut(s) 421
BseBI CCWGG 1 cut(s) 189
BseGI GGATG 4 cut(s) 255, 297, 650, 723
BseLI CCNNNNNNNGG 2 cut(s) 307, 505
BseMI GCAATG 4 cut(s) 74, 113, 510, 815
BseMII CTCAG 2 cut(s) 428, 765
BseNI ACTGG 3 cut(s) 60, 329, 606
BsePI GCGCGC 1 cut(s) 281
BseRI GAGGAG 3 cut(s) 194, 402, 818
BseXI GCAGC 5 cut(s) 234, 236, 262, 278, 573
Bsh1236I CGCG 2 cut(s) 79, 283
Bsh1285I CGRYCG 1 cut(s) 855
BshFI GGCC 4 cut(s) 197, 359, 363, 901
BsiEI CGRYCG 1 cut(s) 855
BsiHKAI GWGCWC 2 cut(s) 657, 869
BsiSI CCGG 4 cut(s) 360, 422, 486, 740
BslI CCNNNNNNNGG 2 cut(s) 307, 505
BsmAI GTCTC 1 cut(s) 337
BsnI GGCC 4 cut(s) 197, 359, 363, 901
Bsp1286I GDGCHC 3 cut(s) 639, 657, 869
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 3 cut(s) 547, 852, 884
BspANI GGCC 4 cut(s) 197, 359, 363, 901
BspCNI CTCAG 2 cut(s) 427, 766
BspEI TCCGGA 1 cut(s) 421
BspFNI CGCG 2 cut(s) 79, 283
BspMAI CTGCAG 2 cut(s) 252, 770
BspOI GCTAGC 1 cut(s) 851
BspPI GGATC 1 cut(s) 892
BsrBI CCGCTC 1 cut(s) 240
BsrDI GCAATG 4 cut(s) 74, 113, 510, 815
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 3 cut(s) 60, 329, 606
BssAI RCCGGY 1 cut(s) 359
BssHII GCGCGC 1 cut(s) 281
BssMI GATC 3 cut(s) 547, 852, 884
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 218
BstACI GRCGYC 1 cut(s) 18
BstBAI YACGTR 1 cut(s) 553
BstC8I GCNNGC 5 cut(s) 199, 283, 361, 657, 849
BstDEI CTNAG 2 cut(s) 414, 774
BstF5I GGATG 4 cut(s) 255, 297, 650, 723
BstFNI CGCG 2 cut(s) 79, 283
BstH2I RGCGCY 2 cut(s) 58, 236
BstHHI GCGC 5 cut(s) 57, 235, 255, 283, 285
BstKTI GATC 3 cut(s) 550, 855, 887
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 3 cut(s) 547, 852, 884
BstMCI CGRYCG 1 cut(s) 855
BstMWI GCNNNNNNNGC 7 cut(s) 198, 203, 230, 287, 375, 661, 814
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 2 cut(s) 668, 832
BstSCI CCNGG 1 cut(s) 187
BstSFI CTRYAG 2 cut(s) 248, 766
BstUI CGCG 2 cut(s) 79, 283
BstV1I GCAGC 5 cut(s) 234, 236, 262, 278, 573
BstV2I GAAGAC 2 cut(s) 21, 825
BsuRI GGCC 4 cut(s) 197, 359, 363, 901
BtgZI GCGATG 1 cut(s) 350
BtsCI GGATG 4 cut(s) 255, 297, 650, 723
BtsI GCAGTG 1 cut(s) 119
BtsIMutI CAGTG 2 cut(s) 119, 613
Cac8I GCNNGC 5 cut(s) 199, 283, 361, 657, 849
CfoI GCGC 5 cut(s) 57, 235, 255, 283, 285
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 899
CseI GACGC 1 cut(s) 15
Csp6I GTAC 2 cut(s) 352, 491
CviAII CATG 3 cut(s) 193, 665, 829
CviQI GTAC 2 cut(s) 352, 491
DdeI CTNAG 2 cut(s) 414, 774
DpnI GATC 3 cut(s) 549, 854, 886
DpnII GATC 3 cut(s) 547, 852, 884
EaeI YGGCCR 2 cut(s) 195, 361
EciI GGCGGA 3 cut(s) 167, 193, 332
Ecl136II GAGCTC 1 cut(s) 655
Eco24I GRGCYC 2 cut(s) 639, 657
Eco53kI GAGCTC 1 cut(s) 655
Eco72I CACGTG 1 cut(s) 553
EcoICRI GAGCTC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 187
EcoT38I GRGCYC 2 cut(s) 639, 657
FaeI CATG 3 cut(s) 196, 668, 832
FaiI YATR 5 cut(s) 194, 666, 685, 729, 830
FatI CATG 3 cut(s) 192, 664, 828
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 8 cut(s) 207, 225, 228, 231, 248, 251, 267, 562
FokI GGATG 4 cut(s) 242, 284, 657, 730
FriOI GRGCYC 2 cut(s) 639, 657
FseI GGCCGGCC 1 cut(s) 363
Fsp4HI GCNGC 8 cut(s) 207, 225, 228, 231, 248, 251, 267, 562
FspBI CTAG 2 cut(s) 722, 848
GlaI GCGC 5 cut(s) 56, 234, 254, 282, 284
GluI GCNGC 8 cut(s) 207, 225, 228, 231, 248, 251, 267, 562
GsuI CTGGAG 2 cut(s) 43, 164
HaeII RGCGCY 2 cut(s) 58, 236
HaeIII GGCC 4 cut(s) 197, 359, 363, 901
HapII CCGG 4 cut(s) 360, 422, 486, 740
HgaI GACGC 1 cut(s) 15
HhaI GCGC 5 cut(s) 57, 235, 255, 283, 285
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 3 cut(s) 196, 668, 832
Hin6I GCGC 5 cut(s) 55, 233, 253, 281, 283
HinP1I GCGC 5 cut(s) 55, 233, 253, 281, 283
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 81, 617
HpaII CCGG 4 cut(s) 360, 422, 486, 740
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 3 cut(s) 352, 520, 601
Hpy188I TCNGA 4 cut(s) 16, 302, 794, 838
Hpy188III TCNNGA 7 cut(s) 143, 259, 403, 422, 545, 568, 642
Hpy8I GTNNAC 3 cut(s) 352, 520, 601
Hpy99I CGWCG 4 cut(s) 20, 23, 218, 404
HpyAV CCTTC 6 cut(s) 58, 142, 167, 256, 488, 577
HpyCH4III ACNGT 1 cut(s) 218
HpyCH4IV ACGT 5 cut(s) 18, 387, 552, 755, 873
HpyCH4V TGCA 6 cut(s) 106, 250, 457, 503, 768, 783
HpyF10VI GCNNNNNNNGC 7 cut(s) 198, 203, 230, 287, 375, 661, 814
HpyF3I CTNAG 2 cut(s) 414, 774
HpySE526I ACGT 5 cut(s) 18, 387, 552, 755, 873
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 3 cut(s) 196, 668, 832
HspAI GCGC 5 cut(s) 55, 233, 253, 281, 283
Kpn2I TCCGGA 1 cut(s) 421
KroI GCCGGC 1 cut(s) 359
KroNI GCCGGC 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 3 cut(s) 547, 852, 884
LmnI GCTCC 9 cut(s) 62, 181, 206, 240, 345, 415, 652, 805, 864
Lsp1109I GCAGC 5 cut(s) 234, 236, 262, 278, 573
LweI GCATC 4 cut(s) 93, 264, 573, 708
MaeI CTAG 2 cut(s) 722, 848
MaeII ACGT 5 cut(s) 18, 387, 552, 755, 873
MaeIII GTNAC 1 cut(s) 667
MalI GATC 3 cut(s) 549, 854, 886
MbiI CCGCTC 1 cut(s) 240
MboI GATC 3 cut(s) 547, 852, 884
MboII GAAGA 4 cut(s) 21, 541, 626, 825
MfeI CAATTG 1 cut(s) 711
MhlI GDGCHC 3 cut(s) 639, 657, 869
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 6 cut(s) 124, 451, 623, 711, 879, 928
MluNI TGGCCA 1 cut(s) 197
MlyI GAGTC 1 cut(s) 90
MmeI TCCRAC 1 cut(s) 39
MnlI CCTC 7 cut(s) 172, 349, 418, 423, 649, 769, 796
Mox20I TGGCCA 1 cut(s) 197
MroI TCCGGA 1 cut(s) 421
MroNI GCCGGC 1 cut(s) 359
MscI TGGCCA 1 cut(s) 197
MseI TTAA 3 cut(s) 87, 590, 750
MslI CAYNNNNRTG 2 cut(s) 302, 872
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 4 cut(s) 360, 422, 486, 740
MspR9I CCNGG 1 cut(s) 189
MunI CAATTG 1 cut(s) 711
MvaI CCWGG 1 cut(s) 189
MvnI CGCG 2 cut(s) 79, 283
MwoI GCNNNNNNNGC 7 cut(s) 198, 203, 230, 287, 375, 661, 814
NaeI GCCGGC 1 cut(s) 361
NdeII GATC 3 cut(s) 547, 852, 884
NgoMIV GCCGGC 1 cut(s) 359
NheI GCTAGC 1 cut(s) 847
NlaIII CATG 3 cut(s) 196, 668, 832
NmeAIII GCCGAG 1 cut(s) 927
NspI RCATGY 2 cut(s) 668, 832
OliI CACNNNNGTG 1 cut(s) 872
PauI GCGCGC 1 cut(s) 281
PdiI GCCGGC 1 cut(s) 361
PfeI GAWTC 1 cut(s) 617
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 8 cut(s) 208, 226, 229, 232, 249, 252, 268, 563
Ple19I CGATCG 1 cut(s) 855
PleI GAGTC 1 cut(s) 89
PmaCI CACGTG 1 cut(s) 553
PmlI CACGTG 1 cut(s) 553
PpsI GAGTC 1 cut(s) 89
Ppu21I YACGTR 1 cut(s) 553
Psp124BI GAGCTC 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 187
PspCI CACGTG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 187
PspPI GGNCC 1 cut(s) 899
PstI CTGCAG 2 cut(s) 252, 770
PsyI GACNNNGTC 1 cut(s) 218
PteI GCGCGC 1 cut(s) 281
PvuI CGATCG 1 cut(s) 855
RigI GGCCGGCC 1 cut(s) 363
RsaI GTAC 2 cut(s) 353, 492
RsaNI GTAC 2 cut(s) 352, 491
RseI CAYNNNNRTG 2 cut(s) 302, 872
SacI GAGCTC 1 cut(s) 657
SaqAI TTAA 3 cut(s) 87, 590, 750
SatI GCNGC 8 cut(s) 207, 225, 228, 231, 248, 251, 267, 562
Sau3AI GATC 3 cut(s) 547, 852, 884
Sau96I GGNCC 1 cut(s) 899
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 3 cut(s) 639, 657, 869
SfaNI GCATC 4 cut(s) 93, 264, 573, 708
SfcI CTRYAG 2 cut(s) 248, 766
SmiMI CAYNNNNRTG 2 cut(s) 302, 872
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 6 cut(s) 124, 451, 623, 711, 879, 928
SspI AATATT 1 cut(s) 894
SspMI CTAG 2 cut(s) 722, 848
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 1 cut(s) 187
TaaI ACNGT 1 cut(s) 218
TaiI ACGT 5 cut(s) 21, 390, 555, 758, 876
TaqI TCGA 4 cut(s) 213, 402, 855, 887
TasI AATT 6 cut(s) 124, 451, 623, 711, 879, 928
TauI GCSGC 3 cut(s) 209, 230, 233
TfiI GAWTC 1 cut(s) 617
Tru1I TTAA 3 cut(s) 87, 590, 750
Tru9I TTAA 3 cut(s) 87, 590, 750
TscAI CASTG 2 cut(s) 119, 613
TseI GCWGC 5 cut(s) 224, 247, 250, 266, 561
TspDTI ATGAA 1 cut(s) 433
TspRI CASTG 2 cut(s) 119, 613
Tth111I GACNNNGTC 1 cut(s) 218
XapI RAATTY 2 cut(s) 451, 623
XceI RCATGY 2 cut(s) 668, 832
XspI CTAG 2 cut(s) 722, 848
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.