RLG00000031031

Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012902
Physical Location & Seq
Reverse (-)
1480290 .. 1482187
1898 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031031

Sequence Viewer

Length: 741 bp
ATGTCCAGCTCCGCCGCAGTCGACGGCGTAGCAGCCACCGCGCTCCGCTCAGTTCTCCACCGCGTCCAGCTGGCAGCCGAAAAGTCCTTCCGCCGCGCCGACGAGATCCGAGTGGTGGCGGTGAGCAAGACGAAGCCGGCGGCCGTCCTCCGCCAAGTCTACGACGCCGGCCACCGCTGCTTCGGCGAGAACTACGTCCAGGAAATCGTCGAGAAAGCTCCTCAGCTTCCTGAAGATATAGAGTGGCATTTTATTGGGAATTTGCAGAGCAATAAAGTAAAGCCTCTTCTAAGTGCTATTCCCAACCTTGCAATGGTAGAGAGTGTGGATGATGAGAAGATTGCAAATCGTCTTAATACTGTTGTTTCTAGCATTGGGAGAACGCCTTTGAAGGTCTTGCTCCAAGTGAATACTAGTGGAGAAGAATCAAAATCTGGTGTTGAGCCCTCTGGATGTGTGGAGCTCGCAAAGCATGTGAGTTTGGATTGTCCAAACCTTGAGTTTTGTGGTCTAATGACCATTGGTATGTTGGATTATTCATCTACACCAGAAAACTTTAAGACATTAGCCAACTGCAGAGCGGAGGTCTGCCAGGCACTTGGAATTCCAGAAGAGAAATGTGAGCTATCAATGGGCATGTCTGCGGACTTTGACCTAGCTATTGAAATGGGAAGCACAAATGTGAGAATTGGATCGACAATTTTTGGGCCAAGAGAATATCAAAAGAAACAATCGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ala_racemase_N

Protein Analysis

247

Amino Acids

26.75

Weight (kDa)

5.51

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ala_racemase_N PF01168 20 - 237 9.8e-16 Alanine racemase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012902)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11930 AT1G11930
fragaria_vesca FvH4_3g01630 FvH4_3g01630 FvH4_3g01630
malus_domestica MD05G1350700.v1.1 MD10G1325400.v1.1
prunus_persica Prupe.4G016700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0002421
rosa_laevigata RLG00000031031
rosa_multiflora Rmu_sc0001717.1_g000005
rosa_roxburghii Rroxscaffold_1G00073430
rosa_rugosa Rorug04G0396100
rosa_samantha Rh5AG019000 Rh5BG021900 Rh5CG020900 Rh5DG020000
rosa_wichuraiana Rw5G001780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 48, 581
AccI GTMKAC 2 cut(s) 21, 159
AccII CGCG 3 cut(s) 41, 63, 96
AclWI GGATC 2 cut(s) 100, 700
AcoI YGGCCR 2 cut(s) 141, 169
AcsI RAATTY 2 cut(s) 259, 603
AcuI CTGAAG 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 115, 313
AgsI TTSAA 2 cut(s) 391, 665
AhlI ACTAGT 1 cut(s) 413
AjnI CCWGG 2 cut(s) 198, 591
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 7 cut(s) 9, 70, 218, 226, 463, 625, 659
AluI AGCT 7 cut(s) 9, 70, 218, 226, 463, 625, 659
Alw21I GWGCWC 1 cut(s) 465
AlwI GGATC 2 cut(s) 100, 700
AoxI GGCC 3 cut(s) 141, 169, 707
ApeKI GCWGC 3 cut(s) 32, 74, 177
ApoI RAATTY 2 cut(s) 259, 603
AspLEI GCGC 2 cut(s) 43, 98
AspS9I GGNCC 1 cut(s) 707
AsuHPI GGTGA 1 cut(s) 133
BanII GRGCYC 2 cut(s) 447, 465
BarI GAAGNNNNNNTAC 2 cut(s) 270, 302
Bbv12I GWGCWC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 44, 86, 164
BceAI ACGGC 2 cut(s) 40, 128
BciT130I CCWGG 2 cut(s) 200, 593
BcuI ACTAGT 1 cut(s) 413
BfaI CTAG 3 cut(s) 369, 414, 656
BfmI CTRYAG 1 cut(s) 574
BisI GCNGC 6 cut(s) 15, 33, 75, 94, 141, 178
BlsI GCNGC 6 cut(s) 16, 34, 76, 95, 142, 179
Bme1390I CCNGG 2 cut(s) 200, 593
BmgT120I GGNCC 1 cut(s) 707
BmrFI CCNGG 2 cut(s) 200, 593
Bpu10I CCTNAGC 1 cut(s) 222
BpuEI CTTGAG 1 cut(s) 518
BsaHI GRCGYC 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 313
Bse118I RCCGGY 2 cut(s) 136, 167
Bse3DI GCAATG 1 cut(s) 318
BseBI CCWGG 2 cut(s) 200, 593
BseGI GGATG 2 cut(s) 334, 458
BseLI CCNNNNNNNGG 2 cut(s) 115, 313
BseMI GCAATG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 63, 236
BseRI GAGGAG 1 cut(s) 210
BseX3I CGGCCG 1 cut(s) 141
BseXI GCAGC 3 cut(s) 44, 86, 164
Bsh1236I CGCG 3 cut(s) 41, 63, 96
Bsh1285I CGRYCG 1 cut(s) 144
BshFI GGCC 3 cut(s) 143, 171, 709
BsiEI CGRYCG 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 465
BsiSI CCGG 2 cut(s) 137, 168
BslI CCNNNNNNNGG 2 cut(s) 115, 313
BsnI GGCC 3 cut(s) 143, 171, 709
Bsp1286I GDGCHC 2 cut(s) 447, 465
Bsp143I GATC 2 cut(s) 105, 692
BspANI GGCC 3 cut(s) 143, 171, 709
BspCNI CTCAG 2 cut(s) 62, 235
BspFNI CGCG 3 cut(s) 41, 63, 96
BspMAI CTGCAG 1 cut(s) 578
BspPI GGATC 2 cut(s) 100, 700
BsrBI CCGCTC 2 cut(s) 48, 581
BsrDI GCAATG 1 cut(s) 318
BsrFI RCCGGY 2 cut(s) 136, 167
BssAI RCCGGY 2 cut(s) 136, 167
BssMI GATC 2 cut(s) 105, 692
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 200, 593
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 2 cut(s) 291, 606
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 4 cut(s) 72, 138, 169, 465
BstDEI CTNAG 4 cut(s) 49, 222, 290, 738
BstF5I GGATG 2 cut(s) 334, 458
BstFNI CGCG 3 cut(s) 41, 63, 96
BstHHI GCGC 2 cut(s) 43, 98
BstKTI GATC 2 cut(s) 108, 695
BstMBI GATC 2 cut(s) 105, 692
BstMCI CGRYCG 1 cut(s) 144
BstMWI GCNNNNNNNGC 4 cut(s) 38, 177, 183, 469
BstNI CCWGG 2 cut(s) 200, 593
BstNSI RCATGY 2 cut(s) 476, 640
BstSCI CCNGG 2 cut(s) 198, 591
BstSFI CTRYAG 1 cut(s) 574
BstUI CGCG 3 cut(s) 41, 63, 96
BstV1I GCAGC 3 cut(s) 44, 86, 164
BstX2I RGATCY 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 1 cut(s) 105
BstZI CGGCCG 1 cut(s) 141
BsuRI GGCC 3 cut(s) 143, 171, 709
BtsCI GGATG 2 cut(s) 334, 458
Cac8I GCNNGC 4 cut(s) 72, 138, 169, 465
CfoI GCGC 2 cut(s) 43, 98
Cfr10I RCCGGY 2 cut(s) 136, 167
Cfr13I GGNCC 1 cut(s) 707
CseI GACGC 2 cut(s) 52, 173
CviAII CATG 2 cut(s) 473, 637
DdeI CTNAG 4 cut(s) 49, 222, 290, 738
DpnI GATC 2 cut(s) 107, 694
DpnII GATC 2 cut(s) 105, 692
EaeI YGGCCR 2 cut(s) 141, 169
EagI CGGCCG 1 cut(s) 141
Eam1104I CTCTTC 2 cut(s) 291, 606
EarI CTCTTC 2 cut(s) 291, 606
EciI GGCGGA 2 cut(s) 80, 140
Ecl136II GAGCTC 1 cut(s) 463
EclXI CGGCCG 1 cut(s) 141
Eco24I GRGCYC 2 cut(s) 447, 465
Eco52I CGGCCG 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 463
EcoRI GAATTC 1 cut(s) 603
EcoRII CCWGG 2 cut(s) 198, 591
EcoT38I GRGCYC 2 cut(s) 447, 465
FaeI CATG 2 cut(s) 476, 640
FaiI YATR 4 cut(s) 239, 474, 527, 638
FatI CATG 2 cut(s) 472, 636
FblI GTMKAC 2 cut(s) 21, 159
Fnu4HI GCNGC 6 cut(s) 15, 33, 75, 94, 141, 178
FokI GGATG 2 cut(s) 341, 465
FriOI GRGCYC 2 cut(s) 447, 465
Fsp4HI GCNGC 6 cut(s) 15, 33, 75, 94, 141, 178
FspBI CTAG 3 cut(s) 369, 414, 656
GlaI GCGC 2 cut(s) 42, 97
GluI GCNGC 6 cut(s) 15, 33, 75, 94, 141, 178
HaeIII GGCC 3 cut(s) 143, 171, 709
HapII CCGG 2 cut(s) 137, 168
HgaI GACGC 2 cut(s) 52, 173
HhaI GCGC 2 cut(s) 43, 98
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 2 cut(s) 476, 640
Hin6I GCGC 2 cut(s) 41, 96
HinP1I GCGC 2 cut(s) 41, 96
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 1 cut(s) 425
HpaII CCGG 2 cut(s) 137, 168
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 22, 160
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 4 cut(s) 211, 230, 450, 608
Hpy8I GTNNAC 2 cut(s) 22, 160
Hpy99I CGWCG 4 cut(s) 26, 104, 167, 212
HpyAV CCTTC 2 cut(s) 97, 385
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 4 cut(s) 265, 311, 344, 576
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 177, 183, 469
HpyF3I CTNAG 4 cut(s) 49, 222, 290, 738
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 2 cut(s) 476, 640
HspAI GCGC 2 cut(s) 41, 96
KroI GCCGGC 2 cut(s) 136, 167
KroNI GCCGGC 2 cut(s) 138, 169
Kzo9I GATC 2 cut(s) 105, 692
LmnI GCTCC 5 cut(s) 14, 48, 223, 405, 460
Lsp1109I GCAGC 3 cut(s) 44, 86, 164
MaeI CTAG 3 cut(s) 369, 414, 656
MaeII ACGT 1 cut(s) 195
MalI GATC 2 cut(s) 107, 694
MbiI CCGCTC 2 cut(s) 48, 581
MboI GATC 2 cut(s) 105, 692
MboII GAAGA 5 cut(s) 245, 278, 349, 434, 623
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 2 cut(s) 447, 465
MluCI AATT 4 cut(s) 259, 603, 687, 699
MmeI TCCRAC 1 cut(s) 510
MnlI CCTC 5 cut(s) 158, 231, 294, 457, 577
MroNI GCCGGC 2 cut(s) 136, 167
MseI TTAA 2 cut(s) 354, 558
MslI CAYNNNNRTG 2 cut(s) 524, 680
MspA1I CMGCKG 2 cut(s) 70, 177
MspI CCGG 2 cut(s) 137, 168
MspR9I CCNGG 2 cut(s) 200, 593
MvaI CCWGG 2 cut(s) 200, 593
MvnI CGCG 3 cut(s) 41, 63, 96
MwoI GCNNNNNNNGC 4 cut(s) 38, 177, 183, 469
NaeI GCCGGC 2 cut(s) 138, 169
NdeII GATC 2 cut(s) 105, 692
NgoMIV GCCGGC 2 cut(s) 136, 167
NlaIII CATG 2 cut(s) 476, 640
NspI RCATGY 2 cut(s) 476, 640
OliI CACNNNNGTG 1 cut(s) 680
PdiI GCCGGC 2 cut(s) 138, 169
PfeI GAWTC 1 cut(s) 425
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 6 cut(s) 16, 34, 76, 95, 142, 179
Psp124BI GAGCTC 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 198, 591
PspGI CCWGG 2 cut(s) 198, 591
PspPI GGNCC 1 cut(s) 707
PstI CTGCAG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 105
PvuII CAGCTG 1 cut(s) 70
RseI CAYNNNNRTG 2 cut(s) 524, 680
SacI GAGCTC 1 cut(s) 465
SalI GTCGAC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 354, 558
SatI GCNGC 6 cut(s) 15, 33, 75, 94, 141, 178
Sau3AI GATC 2 cut(s) 105, 692
Sau96I GGNCC 1 cut(s) 707
ScrFI CCNGG 2 cut(s) 200, 593
SduI GDGCHC 2 cut(s) 447, 465
SfcI CTRYAG 1 cut(s) 574
SmiMI CAYNNNNRTG 2 cut(s) 524, 680
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SpeI ACTAGT 1 cut(s) 413
Sse9I AATT 4 cut(s) 259, 603, 687, 699
SspMI CTAG 3 cut(s) 369, 414, 656
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 2 cut(s) 198, 591
TaaI ACNGT 1 cut(s) 361
TaiI ACGT 1 cut(s) 198
TaqI TCGA 4 cut(s) 21, 210, 695, 734
TasI AATT 4 cut(s) 259, 603, 687, 699
TauI GCSGC 3 cut(s) 17, 96, 143
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 2 cut(s) 354, 558
Tru9I TTAA 2 cut(s) 354, 558
TseI GCWGC 3 cut(s) 32, 74, 177
TspDTI ATGAA 1 cut(s) 528
XapI RAATTY 2 cut(s) 259, 603
XceI RCATGY 2 cut(s) 476, 640
XcmI CCANNNNNNNNNTGG 2 cut(s) 310, 526
XmiI GTMKAC 2 cut(s) 21, 159
XspI CTAG 3 cut(s) 369, 414, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.