Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0011452

Physical Location & Seq

Genomic Coordinates Forward (+)

5189935 .. 5194359

Gene Structure 4425 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1040100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGAATGAAGAGCAACGGTCACTCCTTCTGAGCTCCTCGTCTCGCTTCCCGCCTCCACAAGGAGTTAAACTATCTTATGGAACGGCGGGATTCAGAGCGGATGCATCAATCCTCCAATCCACGGTTTACAGGGTGGGAATTCTGGCGGCCCTAAGGGCCGTCAGCACCCAATCAGTGATCGGCCTCATGATCACAGCCTCTCACAACAAAGTCTCTGATAACGGAATCAAGGTTGCCGACCCGAGTGGCGGAATGCTCTCTCAAGATTGGGAGCCGTTCGCTGATACCCTCGCCAATGCTCGTGATCCGCAACAGCTTGTTCATTTGATAGCTGAATTTGTTGAAAACCAAAAAATCGCACTTGATGGAGCAAAATCCGTGGAGATATTGGTGGCAAGAGATACAAGACCGAGTGGAGGATCTCTGGTTGAAGCTGCTAAACAGGGAATCAGTTCAATTCTTGGAGCGGTTGCCCATGACTTGGGAATTTTAACCACTCCACAGCTACACTGGATGGTTCGGGCTAGAAATAAGGGTTTGAAAGTATCAGACATTGATTACTTCAAGCAGCTTTCAAGCTCGTTTAGGTGCTTGGTAGATTTGATTCCTGGTGGAATTCATCCCAGCGACGTGGATGACAAGTTGGTTGTGGATGGGGCAAATGGTGTTGGTGGAGAAAAACTCGAACTTTTGAAGACGATGTTGAATGGTTTGGTTATTGAGACACGGAACTCCGGAAAAGGAGGAGGTGGTGTACTTAATGAAGGAGTCGGTGCTGATTATGTGCAGAAAGAGAAGGTCGTTCCTTGTAGTTTTGGTCCACAGGATGTTGGGATAAGGTGTGCTAGTTTGGACGGTGATGCTGATCGGCTTGTATATTTTATTGTGCCATCTAGAAGTAGTAATAAGATTGAACTCGTTGATGGGGACAAGATTTTATCCTTGTTTGCTATATTCATCAAGGAGCAACTAAGCATTTTGAGTAAGGAAATAGATGTTAATGGAAATAATGATTATCAGTGTCGCCTTGGTATTGTGCAAACAGCTTATGCAAACGGAGCATCCACGGATTATCTCAAACAGTTAGGCCTAGAAGTCACTTTTACTCCTACAGGAGTGAAATACCTTCATGAGAAAGCTGCGGAGTATGATATCGGGATTTACTTTGAAGCAAATGGCCATGGCACCATTTTGTTCTCAGAACATTACTTACGGTGGTTAGAGACCAGGACTACTGTGCTTTCTGATGTAGCTGAAGGTTCAGAACAGCATAAGGCGGCTTTGAGACTCTTGGCTGTTAGTGAATTGATTAACCAGGCTGTCGGGGATGCGTTGAGTGGGGTACTCTTGGTTGAGGCCATTCTGAAACATATGGGATGGTCAATACAGAGGTGGAATGAGCTTTATCAGGATCTACCCAGTAGGCAGCTTAAGGTCAAGGTTATGGACAGAACTGCTGTTGTAACAGAAAATGCAGAAACTGTGGCCGTGACACCCCCTGGCATTCAAGCAGCCATCAACGCTGAAACCGTTAAATACCCTAGAGGCCGATGTTTTATACGCCCATCAGGAACAGAAGATGTCATACGCGTATATGCGGAGGCATCCACACAGAATGCAGCAGACAGCTTAGCAAACTCTGTAGCAAAACTAGTAGATCAGTTTCTTGGGTTTGGAAGCTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005829 GO:0005911 GO:0006040 GO:0006047 GO:0006048 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009987 GO:0018130 GO:0019438 GO:0030054 GO:0034641 GO:0034654 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046349 GO:0046483 GO:0055044 GO:0055086 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 ko01130 map00520 map01100 map01130

Pfam Domains

Protein Families

PGM_PMM_I PGM_PMM_III PGM_PMM_IV

Protein Analysis

562

Amino Acids

60.5

Weight (kDa)

5.52

Isoelectric Point (pI)

32.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PGM_PMM_I	PF02878	57 - 89	9.4e-07	Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
AMG1_III	PF21404	308 - 460	6.7e-53	Phosphoacetylglucosamine mutase AMG1, domain III
PGM_PMM_III	PF02880	308 - 398	1.7e-06	Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
PGM_PMM_IV	PF00408	479 - 550	5.9e-13	Phosphoglucomutase/phosphomannomutase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011452)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G18070
fragaria_vesca	FvH4_5g21180
malus_domestica	MD06G1040100.v1.1 MD16G1269200.v1.1
prunus_persica	Prupe.5G046300_v2.0.a1 Prupe.5G046300_v2.0.a1
pyrus_communis	pycom06g03350
rosa_chinensis	RchiOBHm_Chr7g0208321
rosa_laevigata	RLG00000003227
rosa_multiflora	Rmu_sc0000841.1_g000011 Rmu_sc0000841.1_g000016 Rmu_sc0007869.1_g000029
rosa_roxburghii	Rroxscaffold_3G00250570
rosa_rugosa	Rorug07G0103300 Rorug07G0103400
rosa_samantha	Rh7AG237700 Rh7BG233300 Rh7CG253400 Rh7DG243700
rosa_wichuraiana	Rw7G020260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1184
AccB7I	CCANNNNNTGG	1 cut(s)	481
AccBSI	CCGCTC	2 cut(s)	98, 467
AccII	CGCG	1 cut(s)	1590
AccIII	TCCGGA	1 cut(s)	734
AclWI	GGATC	3 cut(s)	299, 427, 1419
AcoI	YGGCCR	2 cut(s)	1177, 1485
AcsI	RAATTY	4 cut(s)	138, 335, 486, 615
AcuI	CTGAAG	1 cut(s)	1275
AfaI	GTAC	2 cut(s)	756, 1344
AfiI	CCNNNNNNNGG	5 cut(s)	59, 121, 248, 416, 481
AflII	CTTAAG	1 cut(s)	1430
AflIII	ACRYGT	1 cut(s)	1588
AhlI	ACTAGT	1 cut(s)	1651
AjiI	CACGTC	1 cut(s)	631
AjnI	CCWGG	4 cut(s)	607, 1226, 1314, 1498
Alw21I	GWGCWC	1 cut(s)	35
Alw26I	GTCTC	5 cut(s)	45, 217, 716, 1217, 1279
AlwI	GGATC	3 cut(s)	299, 427, 1419
AlwNI	CAGNNNCTG	1 cut(s)	1481
Ama87I	CYCGRG	1 cut(s)	241
Aor13HI	TCCGGA	1 cut(s)	734
AoxI	GGCC	8 cut(s)	147, 156, 181, 1087, 1177, 1356, 1485, 1546
ApeKI	GCWGC	6 cut(s)	434, 568, 1139, 1426, 1511, 1619
ApoI	RAATTY	4 cut(s)	138, 335, 486, 615
Asp700I	GAANNNNTTC	1 cut(s)	451
AspS9I	GGNCC	3 cut(s)	148, 156, 818
AsuHPI	GGTGA	1 cut(s)	869
AvaI	CYCGRG	1 cut(s)	241
AvaII	GGWCC	1 cut(s)	818
AxyI	CCTNAGG	1 cut(s)	152
BalI	TGGCCA	1 cut(s)	1179
BanI	GGYRCC	1 cut(s)	1184
BanII	GRGCYC	1 cut(s)	35
BarI	GAAGNNNNNNTAC	2 cut(s)	1569, 1601
BauI	CACGAG	1 cut(s)	300
BbsI	GAAGAC	1 cut(s)	701
Bbv12I	GWGCWC	1 cut(s)	35
BbvI	GCAGC	6 cut(s)	421, 580, 1126, 1438, 1523, 1631
BccI	CCATC	8 cut(s)	359, 508, 647, 898, 917, 1371, 1523, 1573
BceAI	ACGGC	4 cut(s)	99, 143, 259, 1472
BciT130I	CCWGG	4 cut(s)	609, 1228, 1316, 1500
BclI	TGATCA	1 cut(s)	189
BcoDI	GTCTC	5 cut(s)	45, 217, 716, 1217, 1279
BcuI	ACTAGT	1 cut(s)	1651
BfaI	CTAG	6 cut(s)	525, 846, 894, 1091, 1542, 1652
BfmI	CTRYAG	2 cut(s)	1110, 1641
BfrI	CTTAAG	1 cut(s)	1430
BglI	GCCNNNNNGGC	1 cut(s)	155
BisI	GCNGC	8 cut(s)	147, 435, 569, 1140, 1278, 1427, 1512, 1620
BlpI	GCTNAGC	1 cut(s)	1630
BlsI	GCNGC	8 cut(s)	148, 436, 570, 1141, 1279, 1428, 1513, 1621
Bme1390I	CCNGG	4 cut(s)	609, 1228, 1316, 1500
Bme18I	GGWCC	1 cut(s)	818
BmeT110I	CYCGRG	1 cut(s)	241
BmgBI	CACGTC	1 cut(s)	631
BmgT120I	GGNCC	3 cut(s)	148, 156, 818
BmiI	GGNNCC	2 cut(s)	273, 1186
BmrFI	CCNGG	4 cut(s)	609, 1228, 1316, 1500
BmrI	ACTGGG	1 cut(s)	1413
BmsI	GCATC	6 cut(s)	91, 113, 850, 1070, 1318, 1613
BmuI	ACTGGG	1 cut(s)	1413
BpiI	GAAGAC	1 cut(s)	701
BplI	GAGNNNNNCTC	2 cut(s)	666, 698
Bpu1102I	GCTNAGC	1 cut(s)	1630
BpuEI	CTTGAG	1 cut(s)	246
BsaBI	GATNNNNATC	1 cut(s)	864
BsaI	GGTCTC	1 cut(s)	1217
BsaJI	CCNNGG	6 cut(s)	120, 378, 1027, 1065, 1180, 1498
BsaWI	WCCGGW	1 cut(s)	734
BsaXI	ACNNNNNCTCC	6 cut(s)	6, 36, 738, 768, 1090, 1120
Bsc4I	CCNNNNNNNGG	5 cut(s)	59, 121, 248, 416, 481
Bse1I	ACTGG	2 cut(s)	515, 1419
Bse21I	CCTNAGG	1 cut(s)	152
Bse8I	GATNNNNATC	1 cut(s)	864
BseAI	TCCGGA	1 cut(s)	734
BseBI	CCWGG	4 cut(s)	609, 1228, 1316, 1500
BseDI	CCNNGG	6 cut(s)	120, 378, 1027, 1065, 1180, 1498
BseJI	GATNNNNATC	1 cut(s)	864
BseLI	CCNNNNNNNGG	5 cut(s)	59, 121, 248, 416, 481
BseMII	CTCAG	2 cut(s)	20, 1212
BseNI	ACTGG	2 cut(s)	515, 1419
BseRI	GAGGAG	2 cut(s)	25, 759
BseXI	GCAGC	6 cut(s)	421, 580, 1126, 1438, 1523, 1631
BseYI	CCCAGC	1 cut(s)	623
BsgI	GTGCAG	1 cut(s)	806
Bsh1236I	CGCG	1 cut(s)	1590
BshFI	GGCC	8 cut(s)	149, 158, 183, 1089, 1179, 1358, 1487, 1548
BshNI	GGYRCC	1 cut(s)	1184
BsiHKAI	GWGCWC	1 cut(s)	35
BsiHKCI	CYCGRG	1 cut(s)	241
BsiSI	CCGG	1 cut(s)	735
BslFI	GGGAC	1 cut(s)	941
BslI	CCNNNNNNNGG	5 cut(s)	59, 121, 248, 416, 481
BsmAI	GTCTC	5 cut(s)	45, 217, 716, 1217, 1279
BsmBI	CGTCTC	1 cut(s)	45
BsmFI	GGGAC	1 cut(s)	941
BsmI	GAATGC	3 cut(s)	258, 1503, 1621
BsnI	GGCC	8 cut(s)	149, 158, 183, 1089, 1179, 1358, 1487, 1548
Bso31I	GGTCTC	1 cut(s)	1217
BsoBI	CYCGRG	1 cut(s)	241
Bsp1286I	GDGCHC	1 cut(s)	35
Bsp13I	TCCGGA	1 cut(s)	734
Bsp143I	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
Bsp1720I	GCTNAGC	1 cut(s)	1630
Bsp19I	CCATGG	1 cut(s)	1180
BspANI	GGCC	8 cut(s)	149, 158, 183, 1089, 1179, 1358, 1487, 1548
BspCNI	CTCAG	2 cut(s)	21, 1211
BspEI	TCCGGA	1 cut(s)	734
BspFNI	CGCG	1 cut(s)	1590
BspHI	TCATGA	2 cut(s)	186, 1129
BspLI	GGNNCC	2 cut(s)	273, 1186
BspPI	GGATC	3 cut(s)	299, 427, 1419
BspQI	GCTCTTC	1 cut(s)	3
BspT107I	GGYRCC	1 cut(s)	1184
BspTI	CTTAAG	1 cut(s)	1430
BspTNI	GGTCTC	1 cut(s)	1217
BsrBI	CCGCTC	2 cut(s)	98, 467
BsrI	ACTGG	2 cut(s)	515, 1419
BssECI	CCNNGG	6 cut(s)	120, 378, 1027, 1065, 1180, 1498
BssMI	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
BssSI	CACGAG	1 cut(s)	300
BssT1I	CCWWGG	2 cut(s)	1027, 1180
Bst2BI	CACGAG	1 cut(s)	300
Bst2UI	CCWGG	4 cut(s)	609, 1228, 1316, 1500
Bst4CI	ACNGT	8 cut(s)	18, 124, 857, 1083, 1215, 1237, 1483, 1531
Bst6I	CTCTTC	1 cut(s)	3
BstAFI	CTTAAG	1 cut(s)	1430
BstDEI	CTNAG	5 cut(s)	29, 152, 971, 1198, 1630
BstDSI	CCRYGG	4 cut(s)	120, 378, 1065, 1180
BstENI	CCTNNNNNAGG	1 cut(s)	57
BstFNI	CGCG	1 cut(s)	1590
BstKTI	GATC	7 cut(s)	180, 192, 307, 422, 868, 1414, 1660
BstMAI	GTCTC	5 cut(s)	45, 217, 716, 1217, 1279
BstMBI	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 1058, 1520
BstNI	CCWGG	4 cut(s)	609, 1228, 1316, 1500
BstSCI	CCNGG	4 cut(s)	607, 1226, 1314, 1498
BstSFI	CTRYAG	2 cut(s)	1110, 1641
BstUI	CGCG	1 cut(s)	1590
BstV1I	GCAGC	6 cut(s)	421, 580, 1126, 1438, 1523, 1631
BstV2I	GAAGAC	1 cut(s)	701
BstX2I	RGATCY	2 cut(s)	419, 1411
BstXI	CCANNNNNNTGG	1 cut(s)	631
BstYI	RGATCY	2 cut(s)	419, 1411
Bsu36I	CCTNAGG	1 cut(s)	152
BsuRI	GGCC	8 cut(s)	149, 158, 183, 1089, 1179, 1358, 1487, 1548
BtgI	CCRYGG	4 cut(s)	120, 378, 1065, 1180
BtrI	CACGTC	1 cut(s)	631
BtsIMutI	CAGTG	3 cut(s)	180, 508, 1025
CaiI	CAGNNNCTG	1 cut(s)	1481
CciI	TCATGA	2 cut(s)	186, 1129
Cfr13I	GGNCC	3 cut(s)	148, 156, 818
Csp6I	GTAC	2 cut(s)	755, 1343
CspCI	CAANNNNNGTGG	2 cut(s)	360, 395
CviAII	CATG	4 cut(s)	187, 476, 1130, 1181
CviQI	GTAC	2 cut(s)	755, 1343
DdeI	CTNAG	5 cut(s)	29, 152, 971, 1198, 1630
DpnI	GATC	7 cut(s)	179, 191, 306, 421, 867, 1413, 1659
DpnII	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
EaeI	YGGCCR	2 cut(s)	1177, 1485
Eam1104I	CTCTTC	1 cut(s)	3
EarI	CTCTTC	1 cut(s)	3
EciI	GGCGGA	1 cut(s)	264
Ecl136II	GAGCTC	1 cut(s)	33
Eco130I	CCWWGG	2 cut(s)	1027, 1180
Eco147I	AGGCCT	1 cut(s)	1089
Eco24I	GRGCYC	1 cut(s)	35
Eco31I	GGTCTC	1 cut(s)	1217
Eco32I	GATATC	1 cut(s)	1153
Eco47I	GGWCC	1 cut(s)	818
Eco53kI	GAGCTC	1 cut(s)	33
Eco57I	CTGAAG	1 cut(s)	1275
Eco81I	CCTNAGG	1 cut(s)	152
Eco88I	CYCGRG	1 cut(s)	241
EcoICRI	GAGCTC	1 cut(s)	33
EcoNI	CCTNNNNNAGG	1 cut(s)	57
EcoRI	GAATTC	2 cut(s)	138, 615
EcoRII	CCWGG	4 cut(s)	607, 1226, 1314, 1498
EcoRV	GATATC	1 cut(s)	1153
EcoT14I	CCWWGG	2 cut(s)	1027, 1180
EcoT22I	ATGCAT	1 cut(s)	106
EcoT38I	GRGCYC	1 cut(s)	35
ErhI	CCWWGG	2 cut(s)	1027, 1180
Esp3I	CGTCTC	1 cut(s)	45
FaeI	CATG	4 cut(s)	190, 479, 1133, 1184
FaqI	GGGAC	1 cut(s)	941
FatI	CATG	4 cut(s)	186, 475, 1129, 1180
FauI	CCCGC	2 cut(s)	57, 79
FauNDI	CATATG	1 cut(s)	1371
FbaI	TGATCA	1 cut(s)	189
Fnu4HI	GCNGC	8 cut(s)	147, 435, 569, 1140, 1278, 1427, 1512, 1620
FriOI	GRGCYC	1 cut(s)	35
Fsp4HI	GCNGC	8 cut(s)	147, 435, 569, 1140, 1278, 1427, 1512, 1620
FspBI	CTAG	6 cut(s)	525, 846, 894, 1091, 1542, 1652
GluI	GCNGC	8 cut(s)	147, 435, 569, 1140, 1278, 1427, 1512, 1620
GsaI	CCCAGC	1 cut(s)	627
HaeIII	GGCC	8 cut(s)	149, 158, 183, 1089, 1179, 1358, 1487, 1548
HapII	CCGG	1 cut(s)	735
Hin1II	CATG	4 cut(s)	190, 479, 1133, 1184
HinfI	GANTC	6 cut(s)	90, 225, 447, 604, 768, 1287
HpaII	CCGG	1 cut(s)	735
HphI	GGTGA	1 cut(s)	869
Hpy166II	GTNNAC	3 cut(s)	127, 755, 821
Hpy188I	TCNGA	8 cut(s)	30, 95, 217, 550, 1201, 1246, 1264, 1365
Hpy188III	TCNNGA	9 cut(s)	187, 263, 302, 735, 894, 1130, 1156, 1409, 1569
Hpy8I	GTNNAC	3 cut(s)	127, 755, 821
Hpy99I	CGWCG	1 cut(s)	632
HpyAV	CCTTC	5 cut(s)	35, 758, 790, 1136, 1250
HpyCH4III	ACNGT	8 cut(s)	18, 124, 857, 1083, 1215, 1237, 1483, 1531
HpyCH4IV	ACGT	1 cut(s)	630
HpyCH4V	TGCA	6 cut(s)	104, 787, 1039, 1052, 1475, 1619
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 1058, 1520
HpyF3I	CTNAG	5 cut(s)	29, 152, 971, 1198, 1630
HpySE526I	ACGT	1 cut(s)	630
Hsp92II	CATG	4 cut(s)	190, 479, 1133, 1184
Kpn2I	TCCGGA	1 cut(s)	734
Ksp22I	TGATCA	1 cut(s)	189
Kzo9I	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
LguI	GCTCTTC	1 cut(s)	3
LmnI	GCTCC	6 cut(s)	38, 271, 368, 464, 964, 1058
Lsp1109I	GCAGC	6 cut(s)	421, 580, 1126, 1438, 1523, 1631
LweI	GCATC	6 cut(s)	91, 113, 850, 1070, 1318, 1613
MaeI	CTAG	6 cut(s)	525, 846, 894, 1091, 1542, 1652
MaeII	ACGT	1 cut(s)	630
MaeIII	GTNAC	4 cut(s)	18, 1096, 1462, 1489
MalI	GATC	7 cut(s)	179, 191, 306, 421, 867, 1413, 1659
MbiI	CCGCTC	2 cut(s)	98, 467
MboI	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
MboII	GAAGA	3 cut(s)	20, 706, 1589
MflI	RGATCY	2 cut(s)	419, 1411
MhlI	GDGCHC	1 cut(s)	35
MlsI	TGGCCA	1 cut(s)	1179
MluCI	AATT	6 cut(s)	138, 335, 456, 486, 615, 1304
MluI	ACGCGT	1 cut(s)	1588
MluNI	TGGCCA	1 cut(s)	1179
MlyI	GAGTC	2 cut(s)	777, 1281
Mox20I	TGGCCA	1 cut(s)	1179
Mph1103I	ATGCAT	1 cut(s)	106
MroI	TCCGGA	1 cut(s)	734
MroXI	GAANNNNTTC	1 cut(s)	451
MscI	TGGCCA	1 cut(s)	1179
MseI	TTAA	7 cut(s)	66, 491, 759, 999, 1311, 1431, 1533
Msp20I	TGGCCA	1 cut(s)	1179
MspCI	CTTAAG	1 cut(s)	1430
MspI	CCGG	1 cut(s)	735
MspR9I	CCNGG	4 cut(s)	609, 1228, 1316, 1500
Mva1269I	GAATGC	3 cut(s)	258, 1503, 1621
MvaI	CCWGG	4 cut(s)	609, 1228, 1316, 1500
MvnI	CGCG	1 cut(s)	1590
MwoI	GCNNNNNNNGC	3 cut(s)	155, 1058, 1520
NcoI	CCATGG	1 cut(s)	1180
NdeI	CATATG	1 cut(s)	1371
NdeII	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
NlaIII	CATG	4 cut(s)	190, 479, 1133, 1184
NlaIV	GGNNCC	2 cut(s)	273, 1186
NmuCI	GTSAC	3 cut(s)	18, 1096, 1489
NsiI	ATGCAT	1 cut(s)	106
PagI	TCATGA	2 cut(s)	186, 1129
PceI	AGGCCT	1 cut(s)	1089
PciSI	GCTCTTC	1 cut(s)	3
PcsI	WCGNNNNNNNCGW	1 cut(s)	1527
PctI	GAATGC	3 cut(s)	258, 1503, 1621
PdmI	GAANNNNTTC	1 cut(s)	451
PfeI	GAWTC	4 cut(s)	90, 225, 447, 604
PflMI	CCANNNNNTGG	1 cut(s)	481
PkrI	GCNGC	8 cut(s)	148, 436, 570, 1141, 1279, 1428, 1513, 1621
PleI	GAGTC	2 cut(s)	776, 1281
PpsI	GAGTC	2 cut(s)	776, 1281
Psp124BI	GAGCTC	1 cut(s)	35
Psp6I	CCWGG	4 cut(s)	607, 1226, 1314, 1498
PspFI	CCCAGC	1 cut(s)	623
PspGI	CCWGG	4 cut(s)	607, 1226, 1314, 1498
PspN4I	GGNNCC	2 cut(s)	273, 1186
PspPI	GGNCC	3 cut(s)	148, 156, 818
PsrI	GAACNNNNNNTAC	2 cut(s)	1194, 1226
PstNI	CAGNNNCTG	1 cut(s)	1481
PsuI	RGATCY	2 cut(s)	419, 1411
RsaI	GTAC	2 cut(s)	756, 1344
RsaNI	GTAC	2 cut(s)	755, 1343
SacI	GAGCTC	1 cut(s)	35
SapI	GCTCTTC	1 cut(s)	3
SaqAI	TTAA	7 cut(s)	66, 491, 759, 999, 1311, 1431, 1533
SatI	GCNGC	8 cut(s)	147, 435, 569, 1140, 1278, 1427, 1512, 1620
Sau3AI	GATC	7 cut(s)	177, 189, 304, 419, 865, 1411, 1657
Sau96I	GGNCC	3 cut(s)	148, 156, 818
SchI	GAGTC	2 cut(s)	777, 1281
ScrFI	CCNGG	4 cut(s)	609, 1228, 1316, 1500
SduI	GDGCHC	1 cut(s)	35
SfaNI	GCATC	6 cut(s)	91, 113, 850, 1070, 1318, 1613
SfcI	CTRYAG	2 cut(s)	1110, 1641
SfiI	GGCCNNNNNGGCC	1 cut(s)	155
SinI	GGWCC	1 cut(s)	818
SmlI	CTYRAG	2 cut(s)	261, 1430
SmoI	CTYRAG	2 cut(s)	261, 1430
SpeI	ACTAGT	1 cut(s)	1651
Sse9I	AATT	6 cut(s)	138, 335, 456, 486, 615, 1304
SseBI	AGGCCT	1 cut(s)	1089
SspMI	CTAG	6 cut(s)	525, 846, 894, 1091, 1542, 1652
SstI	GAGCTC	1 cut(s)	35
StuI	AGGCCT	1 cut(s)	1089
StyD4I	CCNGG	4 cut(s)	607, 1226, 1314, 1498
StyI	CCWWGG	2 cut(s)	1027, 1180
TaaI	ACNGT	8 cut(s)	18, 124, 857, 1083, 1215, 1237, 1483, 1531
TaiI	ACGT	1 cut(s)	633
TaqI	TCGA	1 cut(s)	684
TaqII	GACCGA	1 cut(s)	424
TasI	AATT	6 cut(s)	138, 335, 456, 486, 615, 1304
TatI	WGTACW	1 cut(s)	754
TauI	GCSGC	2 cut(s)	149, 1280
TfiI	GAWTC	4 cut(s)	90, 225, 447, 604
Tru1I	TTAA	7 cut(s)	66, 491, 759, 999, 1311, 1431, 1533
Tru9I	TTAA	7 cut(s)	66, 491, 759, 999, 1311, 1431, 1533
TscAI	CASTG	3 cut(s)	180, 515, 1025
TseFI	GTSAC	3 cut(s)	18, 1096, 1489
TseI	GCWGC	6 cut(s)	434, 568, 1139, 1426, 1511, 1619
Tsp45I	GTSAC	3 cut(s)	18, 1096, 1489
TspDTI	ATGAA	7 cut(s)	17, 21, 311, 608, 777, 946, 1118
TspGWI	ACGGA	5 cut(s)	237, 367, 742, 1071, 1082
TspRI	CASTG	3 cut(s)	180, 515, 1025
Van91I	CCANNNNNTGG	1 cut(s)	481
Vha464I	CTTAAG	1 cut(s)	1430
VpaK11BI	GGWCC	1 cut(s)	818
XagI	CCTNNNNNAGG	1 cut(s)	57
XapI	RAATTY	4 cut(s)	138, 335, 486, 615
XbaI	TCTAGA	1 cut(s)	893
XcmI	CCANNNNNNNNNTGG	1 cut(s)	507
XmnI	GAANNNNTTC	1 cut(s)	451
XspI	CTAG	6 cut(s)	525, 846, 894, 1091, 1542, 1652
Zsp2I	ATGCAT	1 cut(s)	106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.