MD06G1052700.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017307
Physical Location & Seq
Forward (+)
7654553 .. 7656970
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1052700.v1.1.491

Sequence Viewer

Length: 789 bp
ATGCATGCCTTGATCTTCTTCATCCTTTTCTCCACTGCCATCTTTCTTGACACACAGGCATCCACTGAAAACTGCACAACTACTAAGTGCTCTCATGGCAAACCCGATATTAGGTTTCCTTTCCAAGTAAGAGGCCGGCACCCCCATCACTGTGGTCTCCCCGGTTTTGAAATCGACTGCAGCAACAACGCTTCCACAATTCACTTCCGATCTTATGGTGAACTGGGCATCAAATCCATCTCGTACGATGCCAGAAGACTAGATCTTCTCGACCCCAAGGGTTGCGTCCATGAAGTCTTTCTTAATCTCAATCTTTCCCTTACCCCGTTTCGATATTACTATGTTCTGAAAGAGTACTCGTATCTCAACTGTTCGGTTACGCTTTCGCCTTCTTTCTCTCAAGTTCCCTGCCTAAGTGGCTCCGGCTATCATGTTTACACTGTGGAGCCTTCCATGGCTGTGCCGAACTTTTGCCGGGTTGTGAAAACTGTTCCCATTCCCTTCGAGTACAGTCCTTATCTTTCTGACAATAGCTTCGGTCTTGGGTTAACTTGGGGCTCAGTTGGGAATCAAGAAACAGGATGTTTCACCATGGCACATGTTAAAGTGGCGTGTATTGCCATTTTAATTTTGGTGCTGGCAGTGCTGGCAAGAATAAGCATGCATCGGTCCAACAAAACACAATGTCGGAGAGAAGGGGACGATCGAATTCAGGTTGAGAAACTACTCGGAGACTACAAAAGCCTGAAGCTTGAAAGGTGTGAAATAAGGCAACTCTACTCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

263

Amino Acids

29.56

Weight (kDa)

8.41

Isoelectric Point (pI)

35.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 89 1.7e-13 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017307)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g19680
malus_domestica MD06G1052700.v1.1
prunus_persica Prupe.5G063400_v2.0.a1
pyrus_communis pycom06g04410
rosa_laevigata RLG00000003465
rosa_multiflora Rmu_sc0002918.1_g000005
rosa_roxburghii Rroxscaffold_3G00253140
rosa_rugosa Rorug07G0083600
rosa_samantha Rh7AG213400 Rh7BG211300 Rh7CG226300 Rh7DG220600
rosa_wichuraiana Rw7G018490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 138
AcsI RAATTY 1 cut(s) 708
AcuI CTGAAG 1 cut(s) 767
AfaI GTAC 3 cut(s) 245, 356, 509
AfiI CCNNNNNNNGG 1 cut(s) 111
AflIII ACRYGT 1 cut(s) 598
AgsI TTSAA 2 cut(s) 170, 755
AluBI AGCT 2 cut(s) 534, 751
AluI AGCT 2 cut(s) 534, 751
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 2 cut(s) 161, 726
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 1 cut(s) 708
ArsI GACNNNNNNTTYG 2 cut(s) 518, 550
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 669
AsuC2I CCSGG 2 cut(s) 162, 476
AsuHPI GGTGA 2 cut(s) 230, 580
AvaII GGWCC 1 cut(s) 669
BanI GGYRCC 1 cut(s) 138
BanII GRGCYC 1 cut(s) 560
BbsI GAAGAC 1 cut(s) 262
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 1 cut(s) 192
BccI CCATC 3 cut(s) 47, 153, 245
BcnI CCSGG 2 cut(s) 162, 476
BcoDI GTCTC 2 cut(s) 161, 726
BfaI CTAG 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 178
BglI GCCNNNNNGGC 1 cut(s) 417
BglII AGATCT 1 cut(s) 262
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 162, 476
Bme18I GGWCC 1 cut(s) 669
BmgT120I GGNCC 1 cut(s) 669
BmiI GGNNCC 3 cut(s) 140, 421, 447
BmrFI CCNGG 2 cut(s) 162, 476
BmrI ACTGGG 1 cut(s) 233
BmsI GCATC 4 cut(s) 68, 237, 238, 673
BmuI ACTGGG 1 cut(s) 233
BpiI GAAGAC 1 cut(s) 262
BpuEI CTTGAG 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 162, 476
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 4 cut(s) 160, 276, 453, 591
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse118I RCCGGY 1 cut(s) 135
Bse1I ACTGG 1 cut(s) 228
BseDI CCNNGG 4 cut(s) 160, 276, 453, 591
BseGI GGATG 3 cut(s) 21, 59, 587
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 228
BseXI GCAGC 1 cut(s) 192
BsgI GTGCAG 1 cut(s) 58
Bsh1285I CGRYCG 1 cut(s) 706
BshFI GGCC 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 138
BsiEI CGRYCG 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 92
BsiSI CCGG 4 cut(s) 136, 162, 423, 475
BsiWI CGTACG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 161, 726
BsmFI GGGAC 1 cut(s) 713
BsnI GGCC 1 cut(s) 135
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 2 cut(s) 92, 560
Bsp143I GATC 4 cut(s) 12, 209, 262, 703
Bsp19I CCATGG 2 cut(s) 453, 591
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 1 cut(s) 572
BspLI GGNNCC 3 cut(s) 140, 421, 447
BspMAI CTGCAG 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 161
BsrFI RCCGGY 1 cut(s) 135
BsrI ACTGG 1 cut(s) 228
BssAI RCCGGY 1 cut(s) 135
BssECI CCNNGG 4 cut(s) 160, 276, 453, 591
BssMI GATC 4 cut(s) 12, 209, 262, 703
BssT1I CCWWGG 3 cut(s) 276, 453, 591
Bst4CI ACNGT 5 cut(s) 152, 371, 442, 490, 512
BstC8I GCNNGC 5 cut(s) 6, 137, 639, 648, 662
BstDEI CTNAG 4 cut(s) 84, 413, 559, 781
BstDSI CCRYGG 2 cut(s) 453, 591
BstF5I GGATG 3 cut(s) 21, 59, 587
BstKTI GATC 4 cut(s) 15, 212, 265, 706
BstMAI GTCTC 2 cut(s) 161, 726
BstMBI GATC 4 cut(s) 12, 209, 262, 703
BstMCI CGRYCG 1 cut(s) 706
BstMWI GCNNNNNNNGC 5 cut(s) 96, 417, 617, 643, 647
BstNSI RCATGY 3 cut(s) 8, 602, 664
BstSCI CCNGG 2 cut(s) 160, 474
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 1 cut(s) 192
BstV2I GAAGAC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 152
BstYI RGATCY 1 cut(s) 262
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 2 cut(s) 453, 591
BtsCI GGATG 3 cut(s) 21, 59, 587
BtsI GCAGTG 2 cut(s) 33, 648
BtsIMutI CAGTG 5 cut(s) 33, 63, 148, 438, 648
Cac8I GCNNGC 5 cut(s) 6, 137, 639, 648, 662
Cfr10I RCCGGY 1 cut(s) 135
Cfr13I GGNCC 1 cut(s) 669
CseI GACGC 1 cut(s) 274
Csp6I GTAC 3 cut(s) 244, 355, 508
CviAII CATG 8 cut(s) 5, 95, 290, 431, 454, 592, 599, 661
CviJI RGCY 9 cut(s) 135, 420, 426, 448, 458, 534, 558, 744, 751
CviKI_1 RGCY 9 cut(s) 135, 420, 426, 448, 458, 534, 558, 744, 751
CviQI GTAC 3 cut(s) 244, 355, 508
DdeI CTNAG 4 cut(s) 84, 413, 559, 781
DpnI GATC 4 cut(s) 14, 211, 264, 705
DpnII GATC 4 cut(s) 12, 209, 262, 703
Eco130I CCWWGG 3 cut(s) 276, 453, 591
Eco24I GRGCYC 1 cut(s) 560
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 767
EcoRI GAATTC 1 cut(s) 708
EcoT14I CCWWGG 3 cut(s) 276, 453, 591
EcoT22I ATGCAT 2 cut(s) 6, 666
EcoT38I GRGCYC 1 cut(s) 560
ErhI CCWWGG 3 cut(s) 276, 453, 591
FaeI CATG 8 cut(s) 8, 98, 293, 434, 457, 595, 602, 664
FalI AAGNNNNNCTT 2 cut(s) 285, 317
FaqI GGGAC 1 cut(s) 713
FatI CATG 8 cut(s) 4, 94, 289, 430, 453, 591, 598, 660
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 3 cut(s) 8, 46, 594
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 1 cut(s) 260
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 135
HapII CCGG 4 cut(s) 136, 162, 423, 475
HgaI GACGC 1 cut(s) 274
Hin1II CATG 8 cut(s) 8, 98, 293, 434, 457, 595, 602, 664
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HindIII AAGCTT 1 cut(s) 749
HinfI GANTC 1 cut(s) 568
HpaI GTTAAC 1 cut(s) 549
HpaII CCGG 4 cut(s) 136, 162, 423, 475
HphI GGTGA 2 cut(s) 230, 580
Hpy166II GTNNAC 3 cut(s) 221, 436, 549
Hpy188I TCNGA 6 cut(s) 209, 348, 526, 690, 731, 788
Hpy188III TCNNGA 3 cut(s) 47, 269, 572
Hpy8I GTNNAC 3 cut(s) 221, 436, 549
HpyAV CCTTC 4 cut(s) 399, 459, 511, 689
HpyCH4III ACNGT 5 cut(s) 152, 371, 442, 490, 512
HpyCH4V TGCA 4 cut(s) 4, 75, 180, 664
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 417, 617, 643, 647
HpyF3I CTNAG 4 cut(s) 84, 413, 559, 781
Hsp92II CATG 8 cut(s) 8, 98, 293, 434, 457, 595, 602, 664
KroI GCCGGC 1 cut(s) 135
KroNI GCCGGC 1 cut(s) 137
KspAI GTTAAC 1 cut(s) 549
Kzo9I GATC 4 cut(s) 12, 209, 262, 703
LmnI GCTCC 2 cut(s) 425, 445
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 4 cut(s) 68, 237, 238, 673
MaeI CTAG 1 cut(s) 260
MaeIII GTNAC 1 cut(s) 376
MalI GATC 4 cut(s) 14, 211, 264, 705
MboI GATC 4 cut(s) 12, 209, 262, 703
MboII GAAGA 4 cut(s) 7, 10, 257, 267
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 92, 560
MluCI AATT 3 cut(s) 198, 627, 708
MmeI TCCRAC 2 cut(s) 668, 696
MnlI CCTC 1 cut(s) 125
Mph1103I ATGCAT 2 cut(s) 6, 666
MroNI GCCGGC 1 cut(s) 135
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 4 cut(s) 303, 548, 603, 626
MslI CAYNNNNRTG 2 cut(s) 150, 458
MspI CCGG 4 cut(s) 136, 162, 423, 475
MspR9I CCNGG 2 cut(s) 162, 476
MwoI GCNNNNNNNGC 5 cut(s) 96, 417, 617, 643, 647
NaeI GCCGGC 1 cut(s) 137
NciI CCSGG 2 cut(s) 162, 476
NcoI CCATGG 2 cut(s) 453, 591
NdeII GATC 4 cut(s) 12, 209, 262, 703
NgoMIV GCCGGC 1 cut(s) 135
NlaIII CATG 8 cut(s) 8, 98, 293, 434, 457, 595, 602, 664
NlaIV GGNNCC 3 cut(s) 140, 421, 447
NsiI ATGCAT 2 cut(s) 6, 666
NspI RCATGY 3 cut(s) 8, 602, 664
PaeI GCATGC 2 cut(s) 8, 664
PciI ACATGT 1 cut(s) 598
PdiI GCCGGC 1 cut(s) 137
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 568
Pfl23II CGTACG 1 cut(s) 243
PkrI GCNGC 1 cut(s) 182
Ple19I CGATCG 1 cut(s) 706
PscI ACATGT 1 cut(s) 598
PspLI CGTACG 1 cut(s) 243
PspN4I GGNNCC 3 cut(s) 140, 421, 447
PspPI GGNCC 1 cut(s) 669
PstI CTGCAG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 262
PvuI CGATCG 1 cut(s) 706
RsaI GTAC 3 cut(s) 245, 356, 509
RsaNI GTAC 3 cut(s) 244, 355, 508
RseI CAYNNNNRTG 2 cut(s) 150, 458
SaqAI TTAA 4 cut(s) 303, 548, 603, 626
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 4 cut(s) 12, 209, 262, 703
Sau96I GGNCC 1 cut(s) 669
ScaI AGTACT 1 cut(s) 356
ScrFI CCNGG 2 cut(s) 162, 476
SduI GDGCHC 2 cut(s) 92, 560
SetI ASST 5 cut(s) 116, 536, 717, 753, 761
SfaNI GCATC 4 cut(s) 68, 237, 238, 673
SfcI CTRYAG 1 cut(s) 178
SinI GGWCC 1 cut(s) 669
SmiMI CAYNNNNRTG 2 cut(s) 150, 458
SmlI CTYRAG 1 cut(s) 399
SmoI CTYRAG 1 cut(s) 399
SphI GCATGC 2 cut(s) 8, 664
Sse9I AATT 3 cut(s) 198, 627, 708
SspMI CTAG 1 cut(s) 260
StyD4I CCNGG 2 cut(s) 160, 474
StyI CCWWGG 3 cut(s) 276, 453, 591
TaaI ACNGT 5 cut(s) 152, 371, 442, 490, 512
TaqI TCGA 5 cut(s) 174, 270, 331, 504, 706
TaqII GACCGA 2 cut(s) 527, 657
TasI AATT 3 cut(s) 198, 627, 708
TatI WGTACW 2 cut(s) 354, 507
TfiI GAWTC 1 cut(s) 568
Tru1I TTAA 4 cut(s) 303, 548, 603, 626
Tru9I TTAA 4 cut(s) 303, 548, 603, 626
TscAI CASTG 5 cut(s) 40, 70, 155, 445, 648
TseI GCWGC 1 cut(s) 180
TspDTI ATGAA 2 cut(s) 10, 306
TspRI CASTG 5 cut(s) 40, 70, 155, 445, 648
VpaK11BI GGWCC 1 cut(s) 669
XapI RAATTY 1 cut(s) 708
XceI RCATGY 3 cut(s) 8, 602, 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 1 cut(s) 260
ZrmI AGTACT 1 cut(s) 356
Zsp2I ATGCAT 2 cut(s) 6, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.