MD06G1079800.v1.1

Actin-depolymerizing factor 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016486
Physical Location & Seq
Reverse (-)
19564125 .. 19565794
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1079800.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGCCAATGCAGCTTCTGGAATGGCTGTGCACGATGACTGCAAACTGAGGTTCAAGGAGCTAAAAGCAAAGAGGAGCTATCGCTTCATTCTGTTCAAGATTGAGCAACAACAAATTGTGGTTGATAAGCTCGGGGAACCTAGTGAGAGCTATGATGATTTCACCTCCAGTTTTCCTGCTGATGAGTGTCGATATGCTGTCTATGATTTCGATTTCACAACCGAAGAGAACTGCCAGAAAAGCAAAATTTTCTTCATTGCATGGTCGCCGGATTCATCAAAGGTGAGGATGAAGATGGTGTATGCTAGTTCCAAGGACAGATTCAAGAGAGAATTGGACGGCATTTCATTTGAATTGCAAGCAACAGATCCAAGTGAGATGAGCTTGGACATAGTGAAAGGGCGAGCCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

138

Amino Acids

15.8

Weight (kDa)

5.5

Isoelectric Point (pI)

57.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 135 2.8e-42 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016486)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02051 FvH4_6g08480
malus_domestica MD06G1079800.v1.1 MD14G1101700.v1.1
pyrus_communis pycom06g07660 pycom14g09820
rosa_chinensis RchiOBHm_Chr7g0198251
rosa_laevigata RLG00000003880
rosa_roxburghii Rroxscaffold_3G00257890
rosa_rugosa Rorug07G0046200
rosa_samantha Rh7AG169800 Rh7BG173000 Rh7CG179500
rosa_wichuraiana Rw7G014850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 246
AgsI TTSAA 4 cut(s) 55, 97, 325, 353
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 6 cut(s) 14, 61, 78, 130, 150, 384
AluI AGCT 6 cut(s) 14, 61, 78, 130, 150, 384
Alw21I GWGCWC 1 cut(s) 33
Alw44I GTGCAC 1 cut(s) 29
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 1 cut(s) 246
AsuHPI GGTGA 2 cut(s) 154, 295
AvaI CYCGRG 1 cut(s) 131
BaeGI GKGCMC 1 cut(s) 33
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 23
BccI CCATC 1 cut(s) 289
BceAI ACGGC 1 cut(s) 355
BfaI CTAG 2 cut(s) 141, 306
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 138
BpmI CTGGAG 1 cut(s) 151
BsaJI CCNNGG 1 cut(s) 312
Bse1I ACTGG 1 cut(s) 168
Bse3DI GCAATG 1 cut(s) 255
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 1 cut(s) 294
BseMI GCAATG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 33
BseXI GCAGC 1 cut(s) 23
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 1 cut(s) 269
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 1 cut(s) 367
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 39
BspLI GGNNCC 1 cut(s) 138
BspPI GGATC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 1 cut(s) 168
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 1 cut(s) 367
BssT1I CCWWGG 1 cut(s) 312
Bst6I CTCTTC 1 cut(s) 219
BstC8I GCNNGC 3 cut(s) 360, 405, 409
BstDEI CTNAG 1 cut(s) 47
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 1 cut(s) 370
BstMBI GATC 1 cut(s) 367
BstMWI GCNNNNNNNGC 2 cut(s) 11, 240
BstSLI GKGCMC 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 294
Cac8I GCNNGC 3 cut(s) 360, 405, 409
CaiI CAGNNNCTG 1 cut(s) 17
CviAII CATG 1 cut(s) 261
CviJI RGCY 9 cut(s) 5, 14, 26, 61, 78, 130, 150, 384, 407
CviKI_1 RGCY 9 cut(s) 5, 14, 26, 61, 78, 130, 150, 384, 407
DdeI CTNAG 1 cut(s) 47
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 131
EcoT14I CCWWGG 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 1 cut(s) 264
FaiI YATR 6 cut(s) 153, 195, 204, 262, 303, 392
FatI CATG 1 cut(s) 260
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 301
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 2 cut(s) 141, 306
GluI GCNGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 151
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 269
Hin1II CATG 1 cut(s) 264
HinfI GANTC 2 cut(s) 272, 321
HpaII CCGG 1 cut(s) 269
HphI GGTGA 2 cut(s) 154, 295
Hpy166II GTNNAC 1 cut(s) 31
Hpy188III TCNNGA 3 cut(s) 18, 97, 325
Hpy8I GTNNAC 1 cut(s) 31
HpyCH4V TGCA 5 cut(s) 11, 31, 42, 260, 358
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 240
HpyF3I CTNAG 1 cut(s) 47
Hsp92II CATG 1 cut(s) 264
Kzo9I GATC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 58, 75
LpnPI CCDG 5 cut(s) 3, 181, 189, 248, 282
Lsp1109I GCAGC 1 cut(s) 23
MaeI CTAG 2 cut(s) 141, 306
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 3 cut(s) 236, 244, 304
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 114, 246, 332, 353
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 42, 66, 175, 279
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 11, 240
NdeII GATC 1 cut(s) 367
NlaIII CATG 1 cut(s) 264
NlaIV GGNNCC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 272, 321
PkrI GCNGC 1 cut(s) 13
PspN4I GGNNCC 1 cut(s) 138
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 1 cut(s) 367
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 367
SduI GDGCHC 1 cut(s) 33
Sse9I AATT 4 cut(s) 114, 246, 332, 353
SspMI CTAG 2 cut(s) 141, 306
StyI CCWWGG 1 cut(s) 312
TaqI TCGA 2 cut(s) 190, 210
TasI AATT 4 cut(s) 114, 246, 332, 353
TfiI GAWTC 2 cut(s) 272, 321
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 5 cut(s) 76, 244, 264, 305, 336
VneI GTGCAC 1 cut(s) 29
XapI RAATTY 1 cut(s) 246
XspI CTAG 2 cut(s) 141, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.