Rh7AG169800

Actin-depolymerizing factor 7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0016486
Physical Location & Seq
Reverse (-)
14603324 .. 14605588
2265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG169800.1

Sequence Viewer

Length: 468 bp
ATGTTTGACAATCGATCAATCGGAATCCAAATGATTAAGCTTTCTGATGTGGTACAGGCCAATGCAGCTTCTGGAATGGCTGTGCACGATGACTGCAAGCTGAGGTTCTTGGAGCTAAAGGCAAAGAGGAGCTATCGTTTCATTTTGTTCAAGATTGAGCAACAGCAAATTGTGGTTGATACGCTCGGGGAACCCAATGCAAGTTACGACGATTTCACCTCCAGTTTTCCTGCTGATGAGTGTCGTTATGCTGTCTATGATTTCGATTTCACTACCGAAGAGAACTGCCAGAAAAGCAAGATTTTCTTCATCGCATGGTCACCGGATACATCAAAGGTGAGGATGAAGATGGTGTATGCAAGTTCCAAGGACAGATTCAAAAGAGAATTGGACGGCATTTCATTTGAATTGCAAGCAACAGATCCAAGTGAGATGAGCTTGGACATAGTGAAAGGGCGAGCCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04360 ko04666 ko04810 ko05133 map04360 map04666 map04810 map05133
Pfam Domains
Protein Families
Cofilin_ADF

Protein Analysis

155

Amino Acids

17.79

Weight (kDa)

5.24

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 25 - 153 8.3e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016486)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g02051 FvH4_6g08480
malus_domestica MD06G1079800.v1.1 MD14G1101700.v1.1
pyrus_communis pycom06g07660 pycom14g09820
rosa_chinensis RchiOBHm_Chr7g0198251
rosa_laevigata RLG00000003880
rosa_roxburghii Rroxscaffold_3G00257890
rosa_rugosa Rorug07G0046200
rosa_samantha Rh7AG169800 Rh7BG173000 Rh7CG179500
rosa_wichuraiana Rw7G014850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 416
AfaI GTAC 1 cut(s) 54
AgsI TTSAA 3 cut(s) 151, 379, 407
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 6 cut(s) 40, 68, 100, 115, 132, 438
AluI AGCT 6 cut(s) 40, 68, 100, 115, 132, 438
Alw21I GWGCWC 1 cut(s) 87
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 1 cut(s) 416
AlwNI CAGNNNCTG 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 1 cut(s) 57
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 65
AsuHPI GGTGA 3 cut(s) 208, 312, 349
AvaI CYCGRG 1 cut(s) 185
BaeGI GKGCMC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 77
BccI CCATC 1 cut(s) 343
BceAI ACGGC 1 cut(s) 409
BciVI GTATCC 1 cut(s) 319
BfuI GTATCC 1 cut(s) 319
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 205
Bpu10I CCTNAGC 1 cut(s) 101
Bsa29I ATCGAT 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 366
BsaWI WCCGGW 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bse1I ACTGG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 1 cut(s) 348
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 222
BseRI GAGGAG 1 cut(s) 142
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 1 cut(s) 77
BshFI GGCC 1 cut(s) 59
BshVI ATCGAT 1 cut(s) 13
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 1 cut(s) 323
BsnI GGCC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 2 cut(s) 14, 421
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 1 cut(s) 93
BspDI ATCGAT 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 192
BspPI GGATC 1 cut(s) 416
BsrI ACTGG 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 2 cut(s) 14, 421
BssT1I CCWWGG 1 cut(s) 366
Bst6I CTCTTC 1 cut(s) 273
BstC8I GCNNGC 4 cut(s) 98, 414, 459, 463
BstDEI CTNAG 1 cut(s) 101
BstEII GGTNACC 1 cut(s) 318
BstF5I GGATG 1 cut(s) 348
BstKTI GATC 2 cut(s) 17, 424
BstMBI GATC 2 cut(s) 14, 421
BstMWI GCNNNNNNNGC 2 cut(s) 65, 294
BstPI GGTNACC 1 cut(s) 318
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 13
BsuI GTATCC 1 cut(s) 319
BsuRI GGCC 1 cut(s) 59
BsuTUI ATCGAT 1 cut(s) 13
BtgZI GCGATG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 348
Cac8I GCNNGC 4 cut(s) 98, 414, 459, 463
CaiI CAGNNNCTG 1 cut(s) 71
ClaI ATCGAT 1 cut(s) 13
Csp6I GTAC 1 cut(s) 53
CviAII CATG 1 cut(s) 315
CviJI RGCY 9 cut(s) 40, 59, 68, 80, 100, 115, 132, 438, 461
CviKI_1 RGCY 9 cut(s) 40, 59, 68, 80, 100, 115, 132, 438, 461
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 1 cut(s) 101
DpnI GATC 2 cut(s) 16, 423
DpnII GATC 2 cut(s) 14, 421
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 366
Eco88I CYCGRG 1 cut(s) 185
Eco91I GGTNACC 1 cut(s) 318
EcoO65I GGTNACC 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 1 cut(s) 318
FaiI YATR 5 cut(s) 249, 258, 316, 357, 446
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 1 cut(s) 314
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 1 cut(s) 355
Fsp4HI GCNGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 205
HaeIII GGCC 1 cut(s) 59
HapII CCGG 1 cut(s) 323
Hin1II CATG 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 2 cut(s) 24, 375
HpaII CCGG 1 cut(s) 323
HphI GGTGA 3 cut(s) 208, 312, 349
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 2 cut(s) 23, 46
Hpy188III TCNNGA 2 cut(s) 72, 151
Hpy8I GTNNAC 1 cut(s) 85
Hpy99I CGWCG 1 cut(s) 212
HpyCH4V TGCA 6 cut(s) 65, 85, 96, 200, 359, 412
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 294
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 1 cut(s) 318
Kzo9I GATC 2 cut(s) 14, 421
LmnI GCTCC 2 cut(s) 112, 129
LpnPI CCDG 6 cut(s) 41, 57, 235, 243, 302, 336
Lsp1109I GCAGC 1 cut(s) 77
MaeIII GTNAC 2 cut(s) 203, 318
MalI GATC 2 cut(s) 16, 423
MboI GATC 2 cut(s) 14, 421
MboII GAAGA 3 cut(s) 290, 298, 358
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 3 cut(s) 168, 386, 407
MnlI CCTC 4 cut(s) 96, 120, 229, 333
MseI TTAA 1 cut(s) 36
MspI CCGG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 65, 294
NdeII GATC 2 cut(s) 14, 421
NlaIII CATG 1 cut(s) 318
NlaIV GGNNCC 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 318
PfeI GAWTC 2 cut(s) 24, 375
PkrI GCNGC 1 cut(s) 67
PspEI GGTNACC 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 192
PstNI CAGNNNCTG 1 cut(s) 71
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 2 cut(s) 14, 421
SduI GDGCHC 1 cut(s) 87
SetI ASST 9 cut(s) 42, 70, 102, 107, 117, 134, 221, 339, 440
Sse9I AATT 3 cut(s) 168, 386, 407
StyI CCWWGG 1 cut(s) 366
TaqI TCGA 2 cut(s) 13, 264
TasI AATT 3 cut(s) 168, 386, 407
TfiI GAWTC 2 cut(s) 24, 375
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 4 cut(s) 130, 298, 359, 390
VneI GTGCAC 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.